Hitachi Ratio Beam

Spectrophotometer
1
U-1900
Model 101
Model 100
Model U-1000
Model U-1500
Model U-1800
Basic model configured by condensing a
great variety of measurement functions into
a compact and simple-to-operate design.
The spectrophotometer is indispensable for quantitative analysis in extensive fields including
biotechnology, new material development and quality control.
Therefore, its measurement accuracy, reliability and other basic performance factors must be absolute.
Hitachi Model U-1900 ratio beam spectrophotometer upgrades the basic performance, and also
simplifies the operating procedure.
Adoption of highly accurate ratio beam optics.
Standard incorporation of validation function
and self-diagnostic function.
A rich variety of functions such as DNA
quantitation, multi-point quantitation
and multi-wavelength measurement
are standard with this model.
Measurement conditions
can be saved.
Installation of the UV
Solutions program*
enables a personal
computer to control
this instrument.
*: Available at option
Measurement methods savable
A maximum of 5 measurement methods can be saved.
This ensures exact and easy re-measurement.
*: Because selection is allowed among plural methods, an analysts burden
can be reduced and necessary time can be shortened substantially.
2
Adoption of ratio beam optics
Ratio beam is a scheme in which part of the beam is branched with a half mirror
and its light quantity is detected with a detector to obtain a signal reference.
Thanks to the ratio beam scheme, an energy change in the light source, a tem-
poral change of optical elements, etc. can be compensated. Therefore, highly
stable photometric values are obtainable. This always ensures reliable results
even in long-time measurement.
Incorporation of stigmatic concave diffraction grating
The optics of this instrument adopts the Seya-Namioka monochromator wide-
spread as a representative concave diffraction grating monochromator.
Because a concave diffraction grating has both beam condensing and dispersing
functions, an optical system can be configured with fewer mirrors. In a spec-
trophotometer, use of fewer mirrors signifies a shorter optical path, thus giving
rise to an aberration-free bright optics.
For elimination of the aberrations which were essentially unavoidable in the past,
a stigmatic concave diffraction grating has been developed by applying Hitachis
original technology. As a result, a higher resolution has been realized.
Detector
Mirror
D2 lamp
WI lamp
Concave diffraction
grating
Entrance
slit
Exit slit
Filter
Mirror
Mirror
Half
mirror
Detector
Sample
Monochromator Sample
compartment
Lens
Large LCD and user-friendly keypad
Display characters are larger than those of the preceding instrument (U-1800).
Spectrum and working curve are easier to see.
In addition, a keypad featuring an easy-to-use key layout has been employed, so condi-
tion setting and measurement can be completed with the briefest possible key operation.
Personal computer-based instrument
control and data processing
High-level data processing can be carried out by controlling the Model
U-1900 from a PC when the optional UV Solutions program is installed.
Also, report generation will be supported through data pasting, transfer,
etc. to another application program.
For connection with a PC, an RS-232C communication cable is
required.
3 4
Basic performance of U-1900
Spectrophotometer U-1900 answering a broad range of measurement needs.
Measured data excellent in accuracy and reliability is assured by stable basic performance.
A large assortment of measurements selectable
including photometry and wavelength scan.
Diverse measurement techniques such as Photometry, Wavelength scan, Time scan, Ratio
Measurement and Multi-wavelength measurement can be used.
Superiority of ratio beam optics
Shown here is a difference in lamp stability
between single beam and ratio beam optics.
In single beam optics, absorbance signal
becomes unstable with the lapse of time. By
contrast, absorbance signal is stable for a
long time in the ratio beam optics where
change in the light source is compensated.
0.000
0.002
0.004
0.006
0.008
0.010
4,000 3,000 2,000 1,000 0 4,000
time (s)
Single beam Ratio beam
A
b
s
o
r
b
a
n
c
e
3,000 2,000 1,000 0
Main menu
Main menu contains the following items for selection of the
respective modes.
Photometry (sample concentration measurement)
Wavelength scan (spectrum measurement)
Time scan (measurement of change with time)
System (instruments self-diagnosis and environment setup)
Data display (expanded display in real time)
Photometry
Absorbance/transmittance measurement mode, ratio mode
and concentration measurement mode are selectable.
Concentration is measured by generating a working curve
from plural standard samples (max. 20 samples) or via fac-
tor input.
Wavelength scan
A spectrum resulting from scanning over a desired wave-
length range from 190 to 1,100nm can be displayed and fol-
lowed by qualitative analysis.
After measurement, peak search, smoothing and other data
processing can be effected.
Time scan
A spectrum can be displayed by scanning of photometric
value (absorbance/transmittance) at a fixed wavelength in a
desired measurement time from 60 to 99.999 sec.
After measurement, peak search, smoothing and other data
processing can be effected.
Data display
Effective for measuring absorbance or
transmittance at a single wavelength.
Wavelength value and measured data
can be displayed in large size.
Ratio calculation
Purity of nucleic acids can be deter-
mined by calculating the ratio at two
wavelengths (A260/A280).
Validation function
The following performance factors can be checked.
Wavelength accuracy
Wavelength setting repeatability
Bandpass
Baseline flatness
Baseline stability
Noise level
Great effect of 4 nm spectral bandpass
Benzene was measured as a sample at spectral bandpasses of
4nm and 5nm, and the respective spectra are compared here. It is
evident that the spectrum at 4nm bandpass is higher in resolution.
Notice the difference at 243nm. A peak was detected at 4nm band-
pass, while it was not detected at 5nm. Thus, a mere 1nm differ-
ence in spectral bandpass will affect analysis significantly.
Example of application
Quantitation of hexavalent chromium
An example of quantitative analysis of hexavalent chromium by
diphenylcarbazide absorptiometry is introduced here.
As a result of creating a working curve within 0 to 0.8g/mL, a corre-
lation coefficient of 0.9999 was obtained, proving an excellent cali-
bration relationship.
Measuring wavelength: 540nm
Concentration of standard solution: 0, 0.04, 0.1, 0.2, 0.5, 0.8g/mL
Reference: Raw data
Conc 0.0 0.04 0.1 0.2 0.5 0.8
Abs 0.000 0.031 0.081 0.162 0.408 0.646
a working curve
Correlation coefficient: 0.9999
5
Various options tailored for specific applica-
tions such as analysis for quality control.
UV Solutions program (P/N 2J1-0310)
The UV Solutions program enables a PC to control the U-1900.
When this program is installed, the following programs are usable.
(A Windows

XP compatible PC needs to be prepared separately.)

Option package program (P/N 2J1-0311)
Capable of performing color calculation and color difference calculation.
Nucleic acid measurement program (P/N 2J1-0316)
Usable for checking extraction and refinement of nucleic acids such as DNA/RNA essential for genetic research.
Data (photometric values at 230, 260 and 280nm), and calculation results (260/280, nucleic acid concentration, pro-
tein concentration, molar concentration) can be displayed collectively on the screen. This program is also intended
for labor saving in refinement work.
GLP/GMP program (P/N 2J1-0317)
The burden of optical instrument performance check can be alleviated.
Item-dependent measurement mode and automatic measurement mode (when no sample is necessary) have also
been prepared. In addition, a judgment tolerance range is settable so as to meet each analytical purpose.
Report generator program (P/N 2J1-0312)
Developed to enable freely customizing the report output format of measurement results. Microsoft

Excel is used for

report output.
UV Navigation program (P/N 2J1-0313)
This software supports automation of users analytical process ranging from measurement to data processing.
Report output to Microsoft

Excel is also supported and reports can be printed in various ways through combination
with the Report Generator.
Windows

XP and Microsoft

Excel are trademarks of Microsoft Corporation, U.S.A.

Microsoft

and Windows

are registered trademarks of U.S. Microsoft Corporation in USA

and other countries.
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Auto sipper
(P/N 3J0-0100)
Effectively usable for quick analysis of multiple samples in
quality control and clinical chemistry. Sipping a sample
from sample tube automatically, this unit assists full
automation of absorbance measurement.
Specifications
Minimum sample volume 0.6mL
Carryover 1% or less
Cell capacity About 50mL
* This sipper is not capable of regulating cell temperature nor connecting
an autosampler.
Micro cell holder
(P/N 3J0-0102)
Specifications
Applicable cell 10mm rectangular cell
Temperature control By circulating water
* Circulating thermostatic oven and cell are not included in this product.
Communication cable
(P/N 121-1521)
Suitable for measurement of trace samples in medical and
biochemical fields.
Cells compatible with this holder are listed below.
Rectangular long path cell holder
(P/N 3J0-0105)
The optical path length of usable rectangular cell is 10, 20,
30, 40, 50 or 100mm.
This holder is mounted to a 4-cell changer for use.
The use of a long path cell is helpful for high-sensitivity
measurement of low-concentration samples.
Water circulated cell holder
(P/N 210-2111)
Water from an externally installed thermostatic oven is cir-
culated through this cell holder to maintain a rectangular cell
at a constant temperature.
A computer and the spectrophotometer main unit need to
be connected with this cable for computer control of mea-
surement using the UV Solutions program.
Specifications
Wavelength range 220 to 950nm
Cell mounting
/demounting repeatability
Within 0.3%T
Noise level Within 0.004Abs (50mL micro-volume cell used)
Cell
Specifications
Part No. Part name Capacity Optical path length
130-0622 50mL micro cell 50mL 10mm
130-0623 25mL micro cell 25mL 5mm
130-0621 5mL micro cell 5mL 0.5mm
NOTICE: For proper operation, follow the instruction manual when using the instrument.
Specifications in this catalog are subject to change with/or without notice, as Hitachi High-Technologies Corporation continues to develop the latest
technologies and products for our customers.
Tokyo, Japan
http://www.hitachi-hitec.com/science/
24-14 Nishi-Shimbashi 1-chome, Minato-ku, Tokyo, 105-8717, Japan
Tel: +81-3-3504-7211 Fax: +81-3-3504-7302
Printed in Japan (H) HTB-E035 2007.1
_ Ij 7
Printed with soy ink on 100% recycled paper.
Specifications Software functions
Optics Seya-Namioka mount monochromator, ratio beam
Wavelength range 190 to 1,100nm
Spectral bandpass 4nm
Stray light 0.05% or less (220nm for NaI, 340nm for NaNO2)
Wavelength accuracy 0.5nm (at 656.1, 486.0nm)
Wavelength setting repeatability 0.3nm
3 to 3Abs
Photometric range 0 to 300%T
Conc: 0.000 to 9.999
Photometric accuracy
0.002Abs (0 to 0.5Abs)
(certified according to 0.004Abs (0.5 to 1.0Abs)
NIST SRM 930)
0.3%T
Photometric repeatability
0.001Abs (0 to 0.5Abs)
(certified according to 0.002Abs (0.5 to 1.0Abs)
NIST SRM 930)
0.15%T
Wavelength scan speed 10, 100, 200, 400, 800, 1,200, 2,400, 3,600nm/min
Baseline stability 0.0004Abs/h (at 500nm)
Noise level 0.00015Abs (at 500nm)
Baseline flatness 0.002Abs (within 200 to 950nm)
Light source WI and D2 lamps
Light source changeover Auto (at 340.0nm)
Detector Silicon photodiode
Display LCD with backlight (12.2cm)
Printer I/F Centronics interface
Serial I/F RS-232C (exclusive for UV Solutions program)
Size (main unit) 380 (W) 643 (D) 311 (H) mm
Weight (main unit) About 27kg
Power supply 100, 115, 220, 230 or 240V, 50/60Hz
Power consumption 300VA
Measurement mode
Photometry
Wavelength scan
Time scan
Multiple-wavelength
Ratio (260/280)
Working curve type
Linear
K factor input
Calculation of correlation coefficient
Spectrum and working curve printout
Condition printout
Spectrum display
Peak/valley detection
Scale expansion/contraction
Smoothing
Validation function
Automatic wavelength calibration
Lamp ignition time