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    Onion Cell Lab - Y9

    Uploaded by

    Victoria Lowman
    This document provides the procedure and results for a lab experiment examining onion cells under a microscope. The procedure involves preparing wet mount slides of onion skin with and without iodine staining to view cell structures. Students are instructed to make observations and diagrams of onion cells at different magnifications, and to answer questions about cell shapes, structures visible with and without staining, the function of cell walls, and differences observed at different magnifications.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd

    Onion Cell Lab - Y9

    Uploaded by

    Victoria Lowman
    4K views4 pages
    This document provides the procedure and results for a lab experiment examining onion cells under a microscope. The procedure involves preparing wet mount slides of onion skin with and without iodine staining to view cell structures. Students are instructed to make observations and diagrams of onion cells at different magnifications, and to answer questions about cell shapes, structures visible with and without staining, the function of cell walls, and differences observed at different magnifications.

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    Onion Cell Lab - Y9

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    This document provides the procedure and results for a lab experiment examining onion cells under a microscope. The procedure involves preparing wet mount slides of onion skin with and without iodine staining to view cell structures. Students are instructed to make observations and diagrams of onion cells at different magnifications, and to answer questions about cell shapes, structures visible with and without staining, the function of cell walls, and differences observed at different magnifications.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    4K views4 pages

    Onion Cell Lab - Y9

    Uploaded by

    Victoria Lowman
    This document provides the procedure and results for a lab experiment examining onion cells under a microscope. The procedure involves preparing wet mount slides of onion skin with and without iodine staining to view cell structures. Students are instructed to make observations and diagrams of onion cells at different magnifications, and to answer questions about cell shapes, structures visible with and without staining, the function of cell walls, and differences observed at different magnifications.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
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    At a glance
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    The experiment aims to observe plant cell structures by preparing and staining onion skin cells. Key structures that can be observed include the cell wall, cell membrane, cytoplasm, and nucleus.

    The aim of the experiment is to observe plant cell structures by preparing and staining onion skin cells.

    The steps involved in preparing the onion skin cells include breaking and flattening the onion slice, placing it on a slide, adding water and covering with a cover slip. For staining, iodine is added and structures within the cells can then be observed.

    Name: _____________________

    Date:
    /
    /

    The Onion Cell: Lab


    Aim (What is it that you want to find out?)

    Materials (What exactly do you need to do this experiment?)


    Whole white onion
    Iodine
    Microscopes
    Dropper
    Microscope slides
    Cover slips
    Method/Procedure (A list of all the steps that you take to do this
    experiment)
    Preparing the wet mount slide of
    onion skin
    1. Break an onion slice in two.
    2. Carefully pull the slice apart.
    3. Use tweezers to pull off a very thin
    piece of onion skin (the thinner, the
    better)
    4. Place the skin in the center of the slide.
    Try to keep it from folding; get it as flat
    as possible.
    5. Add a drop of water to the onion skin
    and cover with a cover slip.
    6. Press the cover slip down carefully to
    remove any air bubbles.
    7. Place the slide on the stage of the
    microscope, set it to low power, adjust
    the focus so the onion slice is clear.
    Draw four or five cells as seen. Label
    the cell walls.
    8. Switch to higher power and try to
    identify the cell membrane, nucleus,
    and cytoplasm.
    Staining the onion cells
    1. Lift up the cover slip and add one or
    two drops of iodine to the slide.
    2. Lower the cover slip and examine the
    cells on higher power.
    3. Iodine stains different parts of the cells

    so that different organelles can be


    seen. Draw and label the cell wall, cell
    membrane, cytoplasm, and nucleus.
    Observations (What do you see?)

    Onion Cell Drawings


    Name: ___________

    Remember:
    Use pencil only
    No sketchy lines
    No shading
    Include magnification

    Conclusions & Discussion


    1. What is the general shape of the onion cells?

    2. Describe what you saw without the stain.

    3. Why is it easier to see the onion cells after they are stained?

    4. All plant cells have cell walls. What is the function of the cell wall?

    5. Count the number of cells that are seen in the field of view under low
    power magnification and also under higher magnification. Compare the
    number of cells observed in each field of view. What difference do you
    see?

    6. What is the job of the nucleus? Were you able to see the cell nuclei?

    7. What would you do differently if you were to do this experiment again?


    What mistakes will you make?

    Scientist: _____________________
    Class: _______________________
    1)
    2)

    Overall
    Report includes name & date
    Report is neat
    Report was handed in on time

    Aim
    Is complete

    3)

    4)
    5)

    Overall
    Title page includes name & date
    Report is neat
    Report was handed in on time

    Aim
    Is complete

    6)

    Observations
    Diagrams neatly completed
    Diagrams in pencil
    Diagrams are accurately labelled
    Observations are clear and detailed
    Conclusion
    Question 1 is correct
    Question 2 is correct
    Question 3 is correct
    Question 4 is correct
    Question 5 is correct
    Question 6 is correct
    Question 7 is correct

    Scientist: _____________________
    Class: _______________________

    ___
    15

    Observations
    Diagrams neatly completed
    Diagrams in pencil
    Diagrams are accurately labelled
    Observations are clear and detailed
    Conclusion
    Question 1 is correct
    Question 2 is correct
    Question 3 is correct
    Question 4 is correct
    Question 5 is correct
    Question 6 is correct
    Question 7 is correct

    ___
    15

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