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    32 20 01

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    acetonaflores
    This document outlines the procedure for determining insoluble dietary fiber content in foods, food products, and feedstuffs. It involves extracting samples with neutral detergent solution and sodium sulfite at boiling temperature for 60 minutes. The residue is then treated with alpha-amylase enzyme solution at 37°C overnight to remove starch. The remaining insoluble dietary fiber is dried, weighed, and reported as a percentage of the original sample weight. The method has been applied and validated for various plant-based foods including legumes, cereals, and their products.

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    32 20 01

    Uploaded by

    acetonaflores
    175 views2 pages
    This document outlines the procedure for determining insoluble dietary fiber content in foods, food products, and feedstuffs. It involves extracting samples with neutral detergent solution and sodium sulfite at boiling temperature for 60 minutes. The residue is then treated with alpha-amylase enzyme solution at 37°C overnight to remove starch. The remaining insoluble dietary fiber is dried, weighed, and reported as a percentage of the original sample weight. The method has been applied and validated for various plant-based foods including legumes, cereals, and their products.

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    32-20-01

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    This document outlines the procedure for determining insoluble dietary fiber content in foods, food products, and feedstuffs. It involves extracting samples with neutral detergent solution and sodium sulfite at boiling temperature for 60 minutes. The residue is then treated with alpha-amylase enzyme solution at 37°C overnight to remove starch. The remaining insoluble dietary fiber is dried, weighed, and reported as a percentage of the original sample weight. The method has been applied and validated for various plant-based foods including legumes, cereals, and their products.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    175 views2 pages

    32 20 01

    Uploaded by

    acetonaflores
    This document outlines the procedure for determining insoluble dietary fiber content in foods, food products, and feedstuffs. It involves extracting samples with neutral detergent solution and sodium sulfite at boiling temperature for 60 minutes. The residue is then treated with alpha-amylase enzyme solution at 37°C overnight to remove starch. The remaining insoluble dietary fiber is dried, weighed, and reported as a percentage of the original sample weight. The method has been applied and validated for various plant-based foods including legumes, cereals, and their products.

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    of 2

    Fiber

    AACC International Method

    32-20.01
    Page 1 of 2

    Insoluble Dietary Fiber


    Final approval October 27, 1982; Reapproval November 3, 1999

    Definition
    To determine the neutral detergent-insoluble dietary fiber of food, food products, and feedstuffs.
    Apparatus
    1. Extraction apparatus with condenser to fit 600-ml tall-form lipless beaker
    and hot plate adjustable to temperature that will bring 200 ml water from 25 to
    rolling boil in 510 min.
    2. Tall-form lipless beakers, 600-ml.
    3. Coarse glass-frit (ASTM 4060 m) 60-ml filter funnels or crucibles.
    4. Air oven maintained at 110130.
    5. Air oven or incubator maintained at 37 2.
    6. Desiccator with efficient desiccant.
    7. Filtering device; suction flask with suitable holder for filtering funnel or
    crucible.
    8. Fine glass wool.
    9. Lyophilizer (if necessary).
    10. Centrifuge.
    11. Grinding apparatus capable of reducing material to 2030 mesh particles.
    Reagents
    1. Sodium lauryl sulfate, U.S. Pharmacopeia.
    2. Disodium ethylenediaminetetraacetate dihydrate (disodium EDTA), reagent
    grade.
    3. Sodium tetraborate, decahydrate (borax), (Na2B4O710H2O), ACS grade.
    4. Disodium phosphate, anhydrous, reagent grade.
    5. Triethylene glycol.
    6. Phosphoric acid, ACS grade.
    7. Sodium sulfite, anhydrous, reagent grade.
    8. Petroleum ether; boiling range 3560.
    9. Sodium dihydrogen phosphate, anhydrous, reagent grade.
    10. Acetone, laboratory grade.
    11. -Amylase.
    12. Toluene, reagent grade.
    Procedure
    1. Prepare neutral detergent solution: Mix 18.61 g disodium EDTA and 6.81 g
    Na2B4O710H2O with 150 ml water and heat until dissolved. Dissolve sodium
    lauryl sulfate (30 g) and 10 ml 2-ethoxyethanol in 700 ml hot water and add to
    first solution. Dissolve 4.56 g Na2HPO4 in 150 ml hot water and add to first
    doi: 10.1094/AACCIntMethod-32-20.01

    Fiber

    AACC International Method

    32-20.01
    Page 2 of 2

    Insoluble Dietary Fiber (continued)


    solution. Adjust pH to 6.97.1 with H3PO4 if necessary. Note: If precipitate
    forms during storage, warm to 60 until precipitate dissolves.
    2. Weigh sample. Air-dry at room temperature or lyophilize if necessary for
    grinding. Extract three times with 25 ml petroleum ether per g of sample if fat
    content is >10%. Prepare samples by grinding to pass 2030 mesh screen (1 mm).
    3. Weigh equivalent of 1.0 g of original sample into beaker. Add in following
    order: a) 100 ml neutral detergent solution (at room temperature); b) 0.50 g
    Na2SO3.
    4. Heat to boiling in 510 min, then reduce heat to maintain even boiling for
    60 min, timed from onset of boiling.
    5. Add 13 g pad of fine glass wool, shaped to form cup, to coarse glass frit
    filter. Dry at 110 at least 4 hr, cool to constant weight in desiccator, tare, and
    pour neutral-detergent fiber residue into center of glass wool while filtering with
    suction. Wash with at least 300 ml 100 water.
    6. With 0.1M Na2HPO4 and 0.1M NaH2PO4, prepare 0.1M sodium phosphate
    buffer. Suspend 2.5% (w/v) -amylase in 0.1M phosphate buffer (pH 7). Centrifuge, decant, and filter through coarse glass-frit filter.
    7. Add enough enzyme solution to filtered neutral-detergent fiber residue
    (from step 5) to more than cover sample (about 50 ml). Allow about 10 ml to
    pass through to displace wash water.
    8. Plug bottom of filter and add several drops of toluene.
    9. Hold at 37 for 18 hr (overnight).
    10. Remove plug, filter residue with suction, and wash with at least 500 ml
    water. Rinse with about 7580 ml acetone, dry at 110 overnight, and cool in
    desiccator to constant weight (24 hr).
    11. Report as percent insoluble dietary fiber:
    (weight of filter + residue) weight of filter
    100
    weight of original sample

    Notes
    1. -Amylase should be chosen with care since some have been shown to
    exhibit residual hemicellulase activity. Amylase may be assayed for activity by
    the method of Sumner as described in Methods of Enzymology, S. P. Colowick
    and N. O. Kaplan, eds., 1955, p. 149.
    2. This method has been applied to a number of food and feed systems
    including materials containing legumes (soy, pea) and cereal grains (oats,
    wheat, corn, and rye). It has been studied collaboratively using bran and flour
    from corn, wheat, soy, and oats.
    Reference
    Goering, H. K., and Van Soest, P. J. 1970. Forage Fiber Analyses. Agriculture Handbook 379. U.S.
    Dept. Agric.

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