Operating Manual DRS2000 (ENG)

Tissue-Tek DRS 2000
Automatic Slide Stainer
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IMPORTANT NOTICE
If this equipment is used in a manner not
specified by the manufacturer, the protection
provided by the equipment may be impaired.
20 Sakura Finetek USA, Inc.

All Rights Reserved
Printed in U.S.A.
Manufactured for:
Sakura Finetek U.S.A., Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Japan Co., Ltd., Tokyo, 103-0023, Japan
Sakura Finetek Europe B.V., !6!LPHENAANDEN2IJN.,
Made in Japan
0000030-01 5HY(
TABLE OF CONTENTS
Section
Page
INTRODUCTION
General Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1
Physical Characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2
Principles of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.6
Safety Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.6
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.7
INSTALLATION
General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1
Environmental Factors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.3
Instrument Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.5
External Connection Terminals and Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.7
Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.8
EXPLANATION OF SOFTWARE AND CUSTOMIZATION OF SETTINGS

Station Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.2
Setting a Start, End, or Wash Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.2
Setting a Programmed End Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.3
Converting a Wash Station to/from a Solution Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.3
Adding a Solution or Staining Method Name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.4
Deleting a Solution or Staining Method Name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.4
Assigning Station Positions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.5
Editing a Staining Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.6
Explanation of Function Keys (for the Edit Screen) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.7
Copying a Staining Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.7
Utility Operations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.8
Error Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.9
ROUTINE OPERATION
Starting a Staining Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.1
Starting a Staining Program in the Continuous Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.2
Starting a Staining Program in Batch Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.2
Operations During Staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.3
Adding Basket Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.3
Monitoring a Staining Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.3
Interrupting or Canceling a Staining Process. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4
Checking the Endtime for a Staining Run in Progress . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.5
End of a Staining Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.5
Revised 3/30/2010
iii
Section
5
TROUBLESHOOTING
Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.2
CARE OF INSTRUMENT
General Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Daily Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Exterior/Interior Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Bi-weekly Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Replacement of the Activated Carbon Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Monthly Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Drying Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Solution Reservoirs and Slide Baskets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Reservoir Trays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.2
Water Supply Strainer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.2
SERVICE AND REPLACEMENT PARTS

Service Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1
When You Have a Problem. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1
Where to Call for Service. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1
Accessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.2
Appendix A
iv
Page
STAINING RECORD
Sample Staining Record. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A.3
Revised 3/30/2010
INTRODUCTION
Section 1
General Description
The DRS 2000 consists of four major components:
The Tissue-Tek DRS 2000 Automatic Slide Stainer is

an automated, random-access stainer which carries out
multiple staining protocols simultaneously . The DRS 2000
(Fig 1-1) features a two-level design which ef ficiently moves
slides to various solutions for optimal performance.
The DRS 2000 software is programmable for up to twenty
different staining methods, accommodating routine H & E,
gyn, non-gyn, and a variety of special stains or any other
user-defined protocol. The operator can program the
instrument to begin staining in either a batch or continuous
mode. The batch mode does not allow another process run
to start until the previous run has finished. In continuous
operation, the user may add basket sets in a random-access
mode, maximizing efficiency and throughput.
the control panel, through which all operations are

controlled
two-levels of solution reservoirs including wash stations
and one dryer station located on the lower level
the robotic arm, which is the transport mechanism for the
slide baskets
fume control system, which uses an activated carbon
filter, for ef fectively removing harmful vapors from inside
the instrument
To begin a staining process, the operator places up to two
slide baskets into a designated start station located on the
lower portion of the instrument. Either a batch or continuous
mode and the desired method may be selected. The robotic
arm picks up the basket set and sequentially moves the
baskets to the programmed stations. In the continuous
mode, as new baskets are added, the robotic arm will pick up
these additional baskets and move them to their
programmed stations accordingly . The closed-system
design, along with the ventilation system, ensure an
essentially fume-free environment outside of the DRS 2000.
Figure 1-1
Front view of the DRS 2000
(cover closed)
Revised 3/30/2010
1.1
INTRODUCTION
Physical Characteristics
Control Panel (Figure 1-2)

The control panel contains the screen display and the
keypad which the operator uses to communicate with the
instrument software. The keypad consists of five function
keys , numeric keys , arrow keys , and a power ON
indicator . The online display of fers the operator visual
information about the status of current operations.

Figure 1-2
1.2
Reagent Reservoir Compartment (Figure 1-3)
Figure 1-3
The reagent reservoirs are arranged on a two-level design.

There are thirteen reservoirs positioned on the upper level
and fourteen on the lower level , plus one drying
station . The lower level accommodates all wash, start,
and end stations. A maximum of five and a minimum of two
wash stations can be used at one time, depending on the
desired set-up. One or two start stations and up to three end
stations may be set as desired. Each reservoir has an
individual lid and handle for ease of filling and transporting.
The reagent capacity is 650 mL; however , two lines molded
on the inside of the reservoirs designate either 650 mL or
620 mL. The minimum volume of solution needed for two full
slide baskets is 620 mL. Numbers located on the platform
dividing the levels identify the appropriate stations .
Revised 3/30/2010
Robotic Arm (Figure 1-4)

The robotic arm can accommodate up to two baskets
of
twenty slides each, at one time. Each basket is secured on
an adapter by a metal hook . The function of the
robotic arm is to move slide baskets to specific stations
based on the user-defined programs. The arm moves on an
x and z axis and ef ficiently carries baskets to the
programmed stations.
Figure 1-4
Fume Control (Figure 1-5)
Figure 1-6
Revised 3/30/2010
The activated carbon filter is part of an ef fective fume

control system . As air circulates throughout the inside of
the instrument, fumes generated from various solutions are
captured and extracted by the carbon filter . The fume
control housing can be accessed through the lower level of
the instrument, behind the reagent reservoirs. A gasket and
protective cover secures the filter and provides adequate
venting of fumes. It is recommended that the carbon filter
be replaced after two weeks with routine operation of the
stainer.
Figure 1-5
1.3
INTRODUCTION
Leveling Feet/Casters (Figure 1-6)
Figure 1-6
The DRS 2000 has four adjustable feet at each corner of

the instrument to ensure proper leveling. Using two people,
gently tilt the instrument onto one side and rotate the
leveling caster clockwise to decrease the height of the
instrument and counterclockwise to increase the height.
Check all leveling casters to ensure proper positioning of
the instrument.
2
Power Switch (Figure 1-6, Figure 1-7)

The power switch is located on the lower , right side of
the instrument. When the switch is moved to the ON
position the green light on the display panel is illuminated.
Brightness/Contrast Dial (Figure 1-6, Figure 1-7)

This dial controls the brightness and the contrast of the

control panel screen.
Figure 1-7
External Connection Terminals (Figure 1-8)

The DRS 2000 has the ability to be connected to an
uninterruptible power supply unit (UPS) and/or an
external alarm device (Not supplied with the instrument).
See page 2.7 for a description of installation procedures.

1.4
Figure 1-8

Revised 3/30/2010
Rear of Instrument (Figure 1-9, Figure 1-10)

The rear of the DRS 2000 shows the inlet for the power
cord on the bottom, right-hand side of the instrument (19). The other end of the cord attaches to an appropriate AC
electrical outlet. The water supply port and the water
supply adjustment valve are both located on the lefthand side of the instrument (Fig. 1-10). The adjustment
valve is used to increase or decrease the pressure of the
water flowing to the instrument. The drain hose port
allows for suf ficient draining of the DRS. A water supply
hose and drain hose are provided in the accessories for
initial setup.
Figure 1-9
Revised 3/30/2010
Figure 1-10
1.5
INTRODUCTION
Principles of Operation
The DRS 2000 is an automated slide stainer designed
for use in both the histology and cytology laboratories. Its
operations include staining of tissue sections mounted on
glass slides, frozen specimens, and cellular specimens,
either non-gynecological or gynecological.
A microprocessor is responsible for controlling the
movement of the robotic arm. It carries the slide baskets to
the appropriate stations, designated by the programs saved
in memory.
Twenty user-defined programs, performing up to fifty
steps each, can be set and stored in memory. The operator
also has the flexibility of creating user-defined names for
both solution and stain methods to personalize all staining
operations.
The staining operations can be performed in either a
batch or continuous mode.
The solution, wash, start, and end stations can be

configured in various arrangements. However , start, end,
and wash stations may only be set on the lower level of the
instrument. A dryer , fixed at the lower , right corner of the
instrument, can be set at the desired temperature in order
to dry slides and remove residual paraf fin prior to staining.
Upon completion of staining methods, the operator is
alerted by an audible alarm to remove the baskets from the
end station(s).
Safety Features
z
In a continuous mode of operation, the user has the

ability to program one of three options at each station for
optimum accuracy.
Exact [z]:
Staining time for that step will be performed

exactly as programmed.
Delta [S]:
Staining time may be extended to some

degree but no more than 20% longer than the
programmed time.
Infinite [ ]: Staining time for that step may be extended

indefinitely.
The above accuracy settings allow the software to
determine time critical steps and move baskets as
efficiently as possible. Consistent stain quality results from
this flexibility. Each station can also be individually set for
agitation. Agitation is referred to as a gentle up and down
movement of the robotic arm in a reagent reservoir . (Under
the utilities function, speed, frequency , and amplitude of
agitation can be selected). The [MIX+] key enables the
operator to set the agitation ON or OFF for each station.
The user can also choose an enhanced wash option at any
station. An enhanced wash can be set to avoid excess
staining after a high concentration solution is used. These
key features enable the user to edit a program to best meet
their individual staining needs.
1.6
The DRS 2000 is designed to ef fectively remove fumes

generated from harmful solutions. It uses an activated
carbon filter to purify the air that it releases from within
the instrument.
Access to the upper level of the stainer is prohibited
during staining by the instrument cover . If the cover is
opened during operation, the audible alarm sounds and
a message appears to close the cover. This protects the
operator and the robotic arm from potential harm.
The instrument contains an alarm that sounds whenever
there is an error condition that stops or interrupts
operation.
The instrument will alert the operator with an audible
alarm when any of the end stations are full and the next
basket is waiting to complete its run.
The DRS 2000 software contains various messages that
inform the user of its current status.
If a solution has exceeded its use limit, a message will
appear to inform the user to change the solution(s) after
all staining runs are completed.
Revised 3/30/2010
Specifications
Continuous Operation:
Basket sets can be continually loaded on demand as the
start station(s) are available for loading.
Power Required:
North America
Model Number: 4929
Europe
Model Number: 4930
4931
Programs/Methods:
115 VAC 10%, 5.0 amps,
60 Hz
Up to 20 programs can be stored in memory

Up to 50 steps can be programmed for each method
Solution Names:
220/240 VAC 10%, 0.3 amps,
50/60 Hz
220/240 VAC 10%, 2.5 amps,
50/60 Hz (with dryer)
Dimensions:
Width115.0 cm (45 in.)
Height 66.0 cm (26 in.)
Depth 49.0 cm (19 in.)
Weight:
101 kgs (223 lbs)
Operating Conditions:
Temperature 10 C to 40 C (50 F to 104 F)
Relative Humidity 30% to 85% (noncondensing)
Atmospheric Pressure 70 to 106 KPa
(10.15 to 15.37 psi)
Capacity/Fill Volumes:
Solution Reservoirs (total) (26)
Wash Reservoirs (5)
Dryer Stations (1)
Fill level/solution reservoirs 620 - 650 mL
Up to 100 solution names can be stored in memory
Staining Method Names:

Up to 50 staining method names can be stored in memory
Facilities:
Water Supply: Tap water
Water Pressure:
Dynamic Pressure: 0.098 to 0.441 MPa
(approx 1.0 to 4.5 kgf/ cm2)
Max. Static Pressure: 0.833 MPa
(8.5 kgf/ cm2)
Water Temperature:
Less than 30C (86 F) [not freezing]
Drain Type:
Method Gravity drain
Capacity More than 15 liters (3.96 gal)/minute
Safety Standards:
Complies with: UL 3101-1, 1st ed.
CAN/CSA-C22.2 No. 1010.1-92
EN 61010-1:93- A1:92 + A2:95
European standards for CE Marking.
Batch Operation:
Up to 40 glass slides in two baskets
(2 baskets = 1 basket set)
Revised 3/30/2010
1.7
Section 2
INSTALLATION
General Information
Unpacking
This section provides information on selecting a proper

location, unpacking, and installing the T issue-Tek DRS
2000 Automatic Slide Stainer . A T issue-Tek instrument
representative or a certified Biomedical Equipment
Technician should perform the installation. The instrument
must be installed correctly to ensure proper operation and
service. Read this complete Operating Manual before
attempting to operate the instrument. Follow all instructions
carefully.
1. Unpack the instrument by removing the large nails along

the bottom of the shipper , then lift the shipper of f the
pallet. Remove all unpacking materials and accessory
boxes prior to moving the instrument off the wooden base.
The DRS 2000 is a precision instrument and must be

handled accordingly. Rough handling or dropping of the
instrument will disturb or damage internal components.
Always handle the instrument with care.
Environmental Factors
Environmental factors influence the selection of a proper
location for the DRS 2000. As with all sensitive electronic
instruments, prolonged exposure to excessive humidity and
temperature should be avoided. T emperature and humidity
should be held relatively constant to obtain the highest
degree of operating stability. The ambient temperature range
for operating the instrument is 10 C to 40 C (50 F to 104
F). The ambient operating humidity range is between 30% to
85% relative humidity.
Locate the instrument in a well-ventilated area, avoiding
exposure to corrosive vapors or temperature extremes.
Avoid proximity to direct sunlight, open windows, ovens,
open flames, hot plates, radiators, and dry ice baths. A void
proximity to any instrument that consumes a high voltage or
large current, including large refrigerators and ovens. The
bench must have a firm level surface capable of supporting at
least 130 kgs (287 lbs.) of weight. Be sure the instrument
will be located near a power source that meets the electrical
requirements (voltage and amperage) specified on the rating
label located on the rear of the instrument. The power
receptacle must be grounded and should be a clean, noisefree, dedicated line. The water supply port is located on the
back of the instrument at the lower left corner . The location
of the DRS 2000 should be positioned to the left of the
external water source making certain the water supply hose
can be properly connected to the instrument and external
water supply.
Revised 3/30/2010
CAUTION: The instrument is very heavy and large;

therefore, it is strongly recommended that it be lifted
and transported by at least two people.
2. After the instrument is unpacked, it is suggested that the
power cord, water supply hose, and drain hose be
secured at the rear of the instrument before placing on a
bench or counter top. (Refer to page 2.5, Instrument
Setup, for detailed instructions).
3. Carefully lift it into place on a firm, level surface in the
designated work area. Be sure that each leveling foot fully
contacts the benchtop and that each foot has been
properly adjusted insuring that the instrument is level.
Turning the height adjustment feet clockwise will lower the
level and turning the feet counterclockwise will increase
the level of the instrument.
WARNING: PLACE THE INSTRUMENT IN A WELL
VENTILATED AREA.
4. Remove all packing material from inside the instrument.
Make certain that all styrofoam is removed from around
the base of the robotic arm. (Refer to page 2.6, Step 5.,
for instructions on removing the bracket from the robotic
arm).
2.1
INSTALLATION
5. The accessory box includes all items necessary for routine
operation. Please verify that all of the following
accessories are included with the instrument. Refer to the
next two pages for a photo of each accessory.
3.
3.
3.
3.
3
3
3
3.
3.
3.
3.
3.
3.
3.
3.
Reagent reservoirs 26
Wash reservoirs 5
Reagent trays (for upper level) 2
Reagent tray (for lower level) 1
Wash reservoir grommets 10 (5 installed)
Wash station plug 3 (2 installed)
Wash nozzle 5 (3 installed)
Reservoir lids 26
Lid for drying station 1
Slide baskets 6
Basket hooks 6
Basket adapters 3
Drain hose 1
Drain hose clamp 1
Drain elbow assembly 1
(includes drain elbow , connecting hose, and two
connecting hose clamps)
Water supply hose set 1
(includes drain hose, washer/filter, and washer)
Activated carbon cartridge filter 1
Filter gasket and metal cover 1 each
Fuse 1
Power cord 1
Labels (Start, End, and PE)
Operating Manual 1
(including W arranty Registration Card U.S.
customers only)
If any of these items are missing, contact your

Customer/Product Support Representative at Sakura Finetek
at (800) 725-8723.
If there is any visible shipping damage to the instrument or
any item, immediately file a complaint with the carrier , then
notify Sakura directly.
2.2
Revised 3/30/2010
Accessories
Wash Reservoir - 5
Reagent Reservoir - 26
Reagent Reservoir Lid - 26

Wash Nozzle - 5
Wash Cap - 3
Wash Reservoir Grommet - 10
Basket Adapter - 3
Basket Hook - 6
Slide Basket - 6
Drain Hose - 1
Revised 3/30/2010
Drain Elbow Assembly

Drain Elbow
Connecting Hose
Connecting Hose Clamps
2.3
INSTALLATION
Activated Carbon Filter Cartridge -1
Water Supply Hose Set -1

(includes drain hose, washer/filter, and washer)
Filter Gasket for Carbon Cartridge - 1
Power Cord - 1
Metal Cover for Carbon Cartridge -1
Reagent Trays (for upper level) - 2
Drain Hose Clamp - 1
Reagent Tray (for lower level) - 1

2.4
Revised 3/30/2010
CAUTION: The robotic arm is secured at the lower left

side of the instrument by a metal bracket and must be
removed before turning on power.
3. To install the drain hose, place the drain hose assembly

onto the drain port and tighten the connecting hose
clamps to ensure hose clamps are properly seated.
Next, the drain hose can be attached to the drain hose
assembly ensuring the clamped end of the hose attaches
to the elbow . Tighten the clamp by using a phillips
screwdriver. The other end of the drain hose should be
routed to a floor or sink drain which is capable of draining
at a rate of 15 liters (or 4 gallons) per minute.
1. Locate the serial number label found on the left side of the
instrument, next to the power cord. W rite the installation
date and instrument serial number on the W arranty
Registration Card found in the front section of this manual.
Completely fill out the Warranty Registration Card and mail
it back to Sakura for registration. The warranty is valid from
1 year after the date of installation.
CAUTION: Make sure that the entire length of the

drain hose is lower than the instrument drain port. It
should be straight and not twisted or looped. Do not
extend the drain hose by coupling it with another
hose. The end of the drain hose should be at least 4
inches (100 mm) or higher from the water surface in
the floor or sink drain.
Instrument Setup
For ease of installation, the power cord, water supply hose,
and drain hose should be secured at the rear of the
instrument prior to placing on a bench or countertop.
2. Before attaching the water supply hose to the

instrument, place the plain washer in the curved end of the
hose and place the screen washer (screen first) in the
straight end of the water supply hose. Next, connect the
preassembled water supply fitting to the instrument, which
is located at the rear, left side of the unit. The water supply
hose may now be placed on the fitting and secured. The
other end of the hose should be securely fixed to a faucet
or water source at the customer location.
CAUTION: When installing the water supply hose,
make sure it is securely connected to the instrument
and the faucet. Failure to do so may allow water
pressure to loosen the connection and cause overflow.
CAUTION: It is recommended that the water supply to
the instrument be turned OFF when the laboratory is
left unattended, i.e., overnight or weekends.
3. To in
Revised 3/30/2010
4. In order for water to begin flowing to the wash reservoirs,

the water supply knob must be opened. T urning the
knob counterclockwise will open the valve and clockwise
will close the valve. When adjusting the water flow for the
first time, please follow the guidelines below:
Number of wash
stations used
Number of
turns
3 turns
2 turns
1/2 turn
1/4 turn
2.5
INSTALLATION
5. To release the robotic arm, cut the tie wrap and gently
raise the arm . Completely remove the two screws
securing the bracket to the left wall . Next, loosen the
two screws securing the bracket to the tower . Lift the
bracket upward then remove it . Retighten the two
screws on the tower .
8. The solution reservoirs may be placed in the upper and

lower sections of the instrument. The wash reservoirs
are pre-set on the lower level. To change a wash station
to/from a solution station, please refer to Section 3, page
3.3. Check to be sure all reservoirs are properly seated.
Labels on the upper level designate each station number.
Start and end labels are included in the accessory box
and should be placed on the lower , inside door once all
start/end stations have been determined.
9. The solution reservoirs can now be filled with the desired
reagents. A molded line on the inside of each container
designates the proper fill level. The lower line (620 mL)
can be used as a fill line if two baskets of forty slides will
always be run. The higher line designates 650 mL and
should be used to ensure adequate solution levels if only
one basket is usually run.

6. The three metal trays enclosed in the accessory box fit
securely on both the right and left side of the upper level
and along the lower level. When installing the tray on the
lower level, insert it below the protruding wash nozzles
with the black plastic arrows facing toward you. These
trays ensure the proper placement of the solution
reservoirs. Insert them so that the edges are facing down.
7. The activated carbon cartridge, filter gasket, and metal
cover can be installed in the lower , rear section of the
DRS 2000. Make sure that the robotic arm is positioned
all the way to the right of the instrument over station 27.
Release the metal cover in the rear of the unit by pressing
the black button on the right. The carbon cartridge can be
installed, making sure that the filter gasket is placed onto
the filter housing first, then the filter and the metal cover
last. To secure in place, put the left end of the filter and
metal cover in first, then secure the right side by pressing
on the black button.
10. If the power cord is not already attached, plug the

instrument power cord into the DRS, located on the rear ,
lower right corner of the instrument and the other end
into a dedicated, grounded electrical power source of the
proper voltage and amperage.
11. Move the power switch, located at the bottom right side
below the keypad, to the ON position. A small green light
on the control panel will illuminate. W ater is introduced
into the instrument for one minute upon power up if the
water supply hose is properly attached to the instrument
external water source, and if the water flow adjustment
valve is in the open position.
NOTE: If water is leaking, close the faucet and the valve
and verify the connections again.
12. The screen on the control panel will display the initial
Main Menu screen.
TM
Tissue-Tek DRS 2000

START MONITOR
EDIT
STATION
MORE
Main Menu Screen
2.6
Revised 3/30/2010
External Connection Terminals

and Options
If desired, the DRS 2000 may be connected to an
uninterruptible power supply unit and to an external alarm
device, (not supplied with instrument).
1. Connection to an uninterrupted power supply unit
(UPS)
To connect the UPS unit, connect the power failure input
terminals of the instrument to the output terminals of the
UPS using a biaxial cable (20 A WG, approximately 3
meters in length). Next, plug the instrument power cord
into the power output receptacle of the UPS. Turn on the
instrument power switch, and then change the [UPS
Connect] default in the System Setup screen from [NO]
to [YES].
2. Connection to an External Alarm Device

If an operational error occurs, the instrument sends an
alarm signal to an external alarm device through the
external alarm output terminals. The external alarm
device connected to these terminals must be able to
meet the following specifications:
The external alarm device must have its own power
source
Rated voltage: 30 Vrms., less then 60VDC (24VDC is
recommended)
Rated current: more than 10mA, less than 1A
Connecting cable: Biaxial cable, approx. 10 meters in
length, 20 AWG
Output terminal structure: Non-voltage relay (C
contact)
When the instrument detects a power failure signal

coming from the UPS, it operates as follows:
The instrument can transmit the following error signals to

the external alarm device:
The instrument will continue normal operation for 5

minutes after receipt of the power failure signal;
however, the heater of the drying station will be
disabled.
ERROR NO. 1
ERROR NO. 2
ERROR NO. 3
ERROR NO. 10
If power is restored within 5 minutes, the instrument

will continue normal operation. The drying station will
then be operational again.
ERROR NO. 11
If the power failure continues over 5 minutes, the

instrument will stop operation while all basket sets
are immersed in their respective stations.
The UPS should have the following specifications:
Back-up capacity: 200 VA, more than 10 minutes
Power failure output terminal: Non-voltage relay
output, or open collector transistor output
During power on: Short signal
During power off: Open signal
[System Error]
[Power Failure]
[Memory Error]
[WARNING: Close the cover.
Pressing exit will abort all runs.
Press resume to continue staining.]
[Robotic arm does not work normally .
Refer to the manual.]
Status of output terminal circuit:
Terminal
Normal Condition
Error Output Conditions
3-4
3-5
Short
Open
Open
Short
3. Duct Connection Adapter (optional)

The optional duct connection adapter (part number AAK23-0631-01) is designed to connect the instrument to
an external air duct in order to exhaust the fumes. The
outer diameter of the adapter is 38 mm.
If the duct from the instrument to the air connection
adapter and the air duct is used, a ventilation fan can be
installed, which is external to the instrument, for more
effective fume control in the laboratory.
Revised 3/30/2010
2.7
INSTALLATION
Precautions
There are several precautions that must be observed
before operating this instrument.
1. Check the fill level of each solution reservoir to ensure
the specified volumes. Overfilling the reservoirs may
cause an overflow and too little solution volume may be
insufficient for adequate staining.
2. Check that the water supply and drain hose are properly
attached. The water supply hose should be securely
connected to the water supply port on the rear, left side of
the instrument and the faucet connection at the sink.
The drain hose should be connected to the instrument at
the rear, left side and checked to be sure it is draining
properly. If the drain hose is not installed correctly , water
may overflow inside the instrument.
Verify the following:
the full length of the drain hose should be lower than
the drain port
the drain hose should be straight, not twisted
the end of the drain hose should be at least 4 inches
or higher from any fluid surface in the sink or drain
3. Check to be certain that the bracket, securing the robotic
arm is removed and the arm can move freely throughout
the instrument.
2.8
Revised 3/30/2010
EXPLANATION OF SOFTWARE/CUSTOMIZATION
General Information
The Tissue-Tek DRS 2000 Automatic Slide Stainer
contains software that can be customized for the routine
work and procedures performed in the laboratory . This
section explains the various operating modes and provides
information on customizing the software. Many of the
procedures in this section will be used upon initial
installation. However , detailed information on the
progression of screen displays and prompts is given here;
therefore, this section should be read thoroughly before
beginning routine operation. Once you are familiar with this
information, Section 4, Routine Operations, can be used as
a quick reference guide for everyday use.
Section 3
When the DRS 2000 instrument is first turned on, the

Main Menu is displayed. The Main Menu screen is the
central screen from which all set up, programming, and utility
functions are accessed. T wo groups of functions are
accessed through the Main Menu. Pressing the [MORE] key
allows the operator to access both groups.
The [START] function key accesses the Start Process
screen, which allows the user to select a method and begin
staining. Once the method has started, the Process Monitor
screen appears on the display , which indicates where each
basket set is located. The [MONIT OR] key cannot be
activated from the Main Menu screen unless the instrument
is actively staining. The [EDIT] key accesses the Staining
Method List screen. Through this screen, names can be
added/deleted and methods can be programmed. The
[STATION] key allows the operator to choose user-defined
and/or factory set solutions. In this screen, the user can
verify or modify the solution names and verify or reset the
solution usage counters. Pressing [MORE] accesses the
second group of functions.
The only function keys on the second group of keys are
the [UTILITY] and the [EXIT] key . The [UTILITY] key
accesses the Utility Function Selection screen, where the
instrument default parameters can be changed and the error
records can be checked. It also enables the operator to set
up the solution configuration from the Station Configuration
screen. When pressed, the [MORE] key will return again to
the first set of functions.
TM
Tissue-Tek DRS 2000

START MONITOR
EDIT
STATION
UTILITY
MORE
MORE
Main Menu Screen
Revised 3/30/2010
3.1
EXPLANATION OF SOFTWARE
Station Configuration
NOTE: The new configuration must be saved before exiting.
The Station Configuration screen allows the operator to

set all solution, wash, start, and end stations, including the
programmed end. The programmed end station is a userdefined end station which may be set on the lower level of
the DRS 2000 and may be programmed at any station other
than a wash station.
Setting a Start, End, Solution, or Wash

Station
To set a Start, End, or Wash station:
1. Press the [MORE] function key from the Main Menu to
access the [UTILITY] key . Then press the [UTILITY] key
and the [SETUP] key to display the message,
NOTE: Start and end stations S1 and E1 will always occupy

the lower station number and S2 and E2 will occupy the
higher station number. Default settings for start stations are
S1 at station 26 and S2 at 27. Default settings for END
stations are E1 at station 14 and E2 at 15.
NOTE: A minimum of one and a maximum of two start/end
stations may be set. Start/end stations may only be set on
the lower level.
NOTE: A minimum of two and a maximum of five wash
stations may be set. If you want to reconfigure the wash
stations, you must also do a hardware change in order to
either convert a wash station to a solution station, or a
solution station to a wash station. (Refer to page 3.3 for
detailed instructions).
INSTRUMENT SETUP.
2. Press the [ST ATION] key which will display the Station
Configuration screen. The cursor will initially be on the
station labeled E (end station) at station 14. The arrow
keys on the keypad will move the cursor to any desired
station location on the upper or lower level; however all
start, end, and wash stations must be positioned on the
lower level of the instrument.
If you would like to change the factory set positions in the
station configuration, use the arrow keys to move to the
desired station location and press the function key(s),
[START], [END], [SOLN], [W ASH], and/or [PGM END] to
complete the solution arrangement. The [PGM END] key
can be accessed by pressing the [MORE] key from the
Station Configuration screen.
9 10 11 12 13
E1 E2 16 17 18 19 20 W1W2 W3 24 25 S1 S2 DRY
SOLN
START
END
WASH
UTILITY
MORE
MORE
Station Configuration Screen
3.2
Revised 3/30/2010
Setting a Programmed End Station

A programmed end (PE) station may be set on the lower
level to allow the operator to set up a user-defined end
station in addition to the fixed end station(s). The
programmed end may be used for the purpose of running
special stains and/or deparaffinizing.
To position a programmed end Station:
1. Press [MORE] to choose the programmed end from the
Station Configuration screen. Use the arrow keys to
select the desired station location and press [PGM END].
NOTE: The programmed end does not need to be used;
however, if you choose to edit a method with a
programmed end station, it must first be set up in the
Station Configuration screen. Once a PE station is set,
that station cannot be used for anything other than a PE
station.
2. After the desired configuration is achieved, press the
[SAVE] function key . The station configuration will be
saved in memory.
The start/end and PE labels provided as an accessory
item may be placed on the inside of the lower door
accordingly.
The following section provides some corrective procedures
and explains display messages pertaining to the station
configuration process.
z
If the station configuration is incomplete, a message will

appear to alert you of an error so that you may modify the
configuration.
For instance, if there are no programmed wash stations, a
display message will appear: A MINIMUM OF TWO WASH
STATIONS MUST BE PROGRAMMED . Pressing the [EXIT]
key will return you to the Station Configuration screen and
allow you to verify and correct solution arrangement.
3. Pressing [EXIT] will enable you to modify the existing

station configuration, [VERIFY] allows you to view the
staining programs which will be deleted and [DELETE]
will delete those programs which are incompatible with an
existing program. If the [DELETE] key is pressed, the
station configuration is then replaced according to the
modification. The display will return to the System Setup
Selection screen.
Converting a wash station to/from a

solution station
Once you have selected the desired station configuration,
you may or may not need to make changes to remove the
factory set wash stations. The wash stations are set at
station positions 21, 22, and 23. W ash stations may only be
set at station positions 21 to 27. A maximum of five and a
minimum of two may be used at one time.
To transform a wash station to a solution station:
1. Remove the wash reservoir(s) from the lower level.
Turning the wash cap counter-clockwise, remove the
wash nozzle and gasket from the instrument. Replace the
wash nozzle with a wash station plug, and secure the plug
and gasket in place using the wash cap. Next, place a
solution reservoir at that position.
To transform a solution station to wash station:
2. Follow the procedure in reverse order . Turning the wash
cap counter-clockwise, remove the wash cap, wash
station plug, and gasket. Replace the wash station plug
with the nozzle and secure all items in place. Install a
wash reservoir at this station.
3. Verify that the station configuration on the display monitor
reflects the proper wash station positions.
If there are already edited staining programs in memory ,

and the new station configuration is incompatible with any
of them, the following message will appear:
THE NEW SOLUTION CONFIGURATION IS INCOMPATIBLE WITH
AT LEAST ONE EXISTING PROGRAM. PRESS EXIT TO
RECONFIGURE, VERIFY TO VIEW OR DELETE TO DELETE
THESE PROGRAMS.
Revised 3/30/2010
3.3
Adding a Solution or Staining Method
Name
This procedure allows you to create and enter user-defined
solution or method names.
To edit a solution or method name:
1. From the Main Menu, press the [EDIT] key . Press the
[MORE] key to access the second group of function keys.
The [NAMES] key, will allow you to add or delete solution
or method names.
2. Press the [NAMES] key to add a solution or method
name.
5. The [BACK SPC] key will delete the last character

entered. The [SHIFT] key will convert the highlighted
character to either upper or lowercase.
NOTE: The space in front of the letter a is used to create
a space in the text.
6. Press the [SA VE] key after all characters have been
entered. The new solution or staining method name(s)
are saved into memory and will be recalled when
assigning solutions and editing programs. Pressing
[EXIT] will return you to the Name Creation Selection
screen and pressing [EXIT] sequentially will return you to
the Main Menu.
3. Press [SOLN+] or [METHOD+] to edit names.
ADD OR DELETE SOLUTION OR METHOD NAMES
Deleting a Solution or Staining Method

Name
To delete an unwanted solution or staining method name:
SOLN+ METHOD+ DELETE REMOVE
EXIT
Name Creation Selection Screen

4. Select each character by using the arrow keys to move
the cursor to the desired letter , number or symbol and
press [ENTER]. The character will appear next to the
Solution/Method name at the top of the display.
ADD NEW SOLUTION NAME
SOLUTION NAME ? HEM
abcdefghijklmnopqrstuvwxyz
0123456789!"#$%&'()*+,-./:;
<=>?@[\]^_{I}
ENTER BACK SPC SHIFT
SAVE
1. Press [DELETE] from the Name Creation screen. Next,

press the [SOLN-] or [METHOD-] key relevant to the
name(s) you wish to delete.
2. The display will either read, DELETE SOLUTION NAME or
DELETE METHOD NAME.
3. Using the up and down ( S T) arrow keys, move the

cursor to highlight the name(s) to be deleted and press
the [DELETE] key. To confirm, press [YES] or [NO].
4. Pressing [EXIT] sequentially will return you to the Main
Menu.
EXIT
Solution Name Creation Screen
3.4
Revised 3/30/2010
or down arrow keys or FUNCTION keys to scroll through

the Solution selection list.
Assigning Solution Positions

From the Solution Configuration screen, you can assign or
modify solutions in the setup configuration. The solutions
stored in memory , either user-defined or factory set, will be
recalled when assigning solutions. Y ou may also use this
screen for solution management. The COUNT column in
this display allows you to set the number of times you would
like each station to be used. The first number under the
count column identifies the set number . The number in
parenthesis increases each time that specific solution is
used. The instrument will keep track of the number of uses
and will alert you when a station needs to be changed. This
function can be on or off for each station. The symbol, _ __
indicates this function is not being used at a given station.
ASSIGN SOLUTIONS TO STATIONS
STA SOLUTION
COUNT
1
XYLENE
---(---)
2
XYLENE
1 ( 10)
3
E-ALC:100%
3 ( 5)
ASSIGN COUNT 0 ALL 0
REMOVE
EXIT
3. To set the solution usage limit, move the cursor to the

Count column by using the right arrow key . Enter a
desired number of times you would like that specific
solution used. You may select a range of (1-999) by using
the numeric keys. If the solution management option is
not needed, press the [DISABLE] key to change the
numbers in the Count column to [---(---)]. The Solution
management option will be disabled for each station
displaying [---(---)]. Pressing the [EXIT] key will return you
to the Main Menu
STA SOLUTION
COUNT
1
XYLENE
---(---)
2
XYLENE
1 ( 10)
3
E-ALC:100%
3 ( 5)
ASSIGN COUNT 0
ALL 0
PRINT
DISABLE COUNT 0
ALL 0
PRINT
EXIT
EXIT
Solution Configuration and Usage Count Screen
Solution Configuration and Usage Count Screen

To set solution positions:
1. From the Main Menu, press the [ST ATION] key. The
display will read, ASSIGN SOLUTIONS TO STATIONS . Move
the cursor to the desired station number by using the up
and down arrow keys and press [ASSIGN]. The Solution
Name Selection screen will appear and the solution list
will appear as a window in the upper right corner of the
display.
STA SOLUTION
COUNT
1
XYLENE
---(---)
2
XYLENE
1 ( 10)
ENTER
UP
SOLUTION LIST
E-ALC: 100%
E-ALC: 50%
E-ALC: 70%
E-ALC: 75%
DOWN
EXIT
Solution Name Selection Screen

2. Using the arrow keys, scroll through the solution list and
select the desired solution for a station by pressing
[ENTER]. The solution name will appear under the
column marked SOLUTION. You may use either the up
Revised 3/30/2010
NOTE: The function key [COUNT 0] will reset the number

at that station to 0. The [ALL 0] key will reset all stations
to 0.
4. When a solution has reached its usage limit, the
message, A SOLUTION HAS REACHED ITS USE LIMIT. PLEASE
CHANGE THE SOLUTION AFTER ALL STAINING RUNS HAVE
ENDED will appear. Y ou may change the appropriate
stations and reset the COUNT column for each station

by pressing [COUNT 0]. If you do not reset the count
column, after the next staining run is completed, you will
be prompted again to exchange solutions after all runs
have ended.
NOTE: Many internal components of the DRS 2000 are

made of stainless steel. These components have a
protective coating which is resistant to most solvents and
reagents used in histological and cytological staining
procedures. Iodine, by nature, will penetrate the
protective coating and allow corrosion to occur . To
enhance the life of the DRS 2000 instrument and its
components, the use of iodine or any other halogen
salt is not recommended.
3.5
Editing a Staining Method
This section describes the process of creating and editing a
staining method. The edit mode allows you to set up a
staining method to best meet your needs by allowing you to
program times, agitation, and other options to enhance stain
quality. Before editing a method, the station configuration
and solution assignment needs to be set up. Please refer to
pages 3.2 and 3.4 to complete these procedures if they have
not already been completed.
To edit a staining program:
1. From the Main Menu, press [EDIT]. The display will read:
SELECT STAIN METHOD. Any method showing an asterisk
* next to the name means that it has already been
edited and saved in memory.
NOTE: If you are editing a method for the first time, the
only * method will be the factory set HE(Sakura)
method.
3. Once the edit screen is displayed, use the arrow keys to

move the cursor to the desired step and the numeric keys
to enter the number of the station position. When a
station number is entered under the column labeled
STA, the corresponding solution from the solution list will
automatically appear.
NOTE: To program a W ash, Dryer, End, or PE station,
use the function keys labeled [W ASH], [DRYER], [END],
and [PE]. Use the [MORE] key to access these functions.
4. The time may be set from (0001 to 9999) for each
station. Set the desired time using the numeric keys,
press [MORE], then [SAVE].
5. The MIX column is used to set agitation or an enhanced
wash at that specific station. Press the [MIX+] key to
display the character + to choose agitation at that
specific station or to choose an enhanced wash at that
station. If nothing is displayed, neither agitation or an
enhanced wash will take place.
2. Move the cursor to the desired method name by using the

up and down arrow keys and press [EDIT]. The Staining
Program Editing screen is displayed. (If you wish to
create a new staining method name, please refer to page
3.4, Adding a Solution or a Staining Method Name.
STP
8
9
10
11
12
STA
10
11
12
13
E*
SOLUTION
E-ALC
XYLENE
XYLENE
DISTILLED WATER
END STATION
EXACT MIX +
WASH
TIME
00'30"
02'00"
03'00"
02'00"
_ _ '_ _"
END
MIX
+
*
+
+
MORE
Staining Program Editing Screen
3.6
Revised 3/30/2010
Explanation of Function Keys

(for the Edit Screen)
The [EXACT] function key is used for the purpose of
establishing critical and non-critical process times for a
specific step. Its main purpose is to create flexibility in
process times which ultimately increase throughput. Each
time the [EXACT] key is pressed, the symbol in front of the
time in the TIME column will change from [z] to [S] to [ ],
then will cycle back through the sequence.
EXACT [z]:
The process time at that step will be

performed exactly as programmed.
DELTA [S]:
The process time for that step may be

extended to some degree but by no more
than 20%.
INFINITE []: The process time at that step may be

extended indefinitely to provide maximum
flexibility.
If there are no time critical steps, the infinite [ ] symbol
may be used at all stations for optimum throughput. Exact
[z] should only be used when the time for a specific solution
should not be modified. The delta [ S] symbol should be
used when a time for a specific station can be lengthened
for no more than 20% of its programmed time.
Copying a Staining Method

To copy one Staining Method in order to create a new
method, follow the procedure below:
1. From the Main Menu press [EDIT]. The Staining Method
List screen is displayed.
2. Use the arrow keys to select the stain method name you
would like to copy from, then press [COPY].
3. The display will read, SELECT STAIN METHOD TO COPY TO.
Use the arrow keys to scroll down and select the method
you would like to copy to and press [ENTER].
4. To verify, press [ENTER].
5. To modify the copied method, press [EDIT] and follow edit
procedures on page 3.6.
6. Press [EXIT] to return to the Main Menu.
The [MIX+] key offers three options:

1 If the [+] symbol is shown, the instrument will agitate in
that station based on the parameters set under the
[UTILITY] function. Mix amplitude, frequency , and
speed can be programmed under the [UTILITY]
function.
2 If the [ *] symbol is displayed, that indicates that the
Enhanced Wash feature is set for that step. The
Enhanced Wash feature can be used to avoid excess
staining when using highly concentrated solutions.
Under the [UTILITY] function you may also set the
enhanced wash frequency and speed.
3 The blank field [ ] indicates that neither mixing or an
enhanced wash will be carried out.
Revised 3/30/2010
3.7
Utility Operations
The [SETUP] function key under utility operations of fers the
user a wide range of user-selected features, which enhance
stain quality. The error log may also be accessed under
[UTILITY] by pressing the [ERR LOG] key.
1. The [UTILITY] function key can be accessed by pressing
the [MORE] key from the Main Menu. The functions
under [SETUP] enable you to set up parameters for all
programs which enhance staining operations.
2. Press in sequence [UTILITY] [SETUP] [MODE].
The Default Parameters screen appears and listed are the
following default settings:
1. MIX AMPLITUDE
25 mm
2. MIX FREQUENCY
3 times
3. MIX SPEED
4. BASKET LIFTING SPEED
5. DRYER TEMPERATURE SET
65C
6. WATER WASH CYCLE TIME
1min 00 sec.
3. Move the cursor to the parameter you would like to

change by using the up and down arrow keys, then press
the [SELECT] key. The parameter options will appear in a
window in the upper right corner of the display.
4. To modify the parameters, move the cursor to the desired
parameter setting by using the up and down arrow keys
and press [ENTER]. The default parameter will change to
the selected option. Follow this procedure for each
parameter you wish to change. After all parameter
changes have been made, press the [EXIT] key . A
message will appear , DO YOU WANT TO SAVE SYSTEM
SETUP? Press the [YES] key to confirm, to cancel, press
the [NO] key. Pressing [EXIT] sequentially will return you
to the Main Menu.
7. ENHANCED WASH FREQUENCY 3 times

8. ENHANCED WASH SPEED
9. UPS CONNECT
NO
10. KEY SOUND
YES
11. PRINTER BOX CONNECT
NO
3.8
Revised 3/30/2010
A description of the parameters and their allowable ranges

are as follows:
ENHANCED WASH SPEED:

The speed at which the robotic arm moves up and down
during the enhanced wash. Range: (1, slowest - 5, fastest)
MIX AMPLITUDE:
The travel distance of the robotic arm during the up and
down movement of the mixing cycle.
Range: 10/15/20/25/30 mm.
MIX FREQUENCY:
The number of times the robotic arms moves up and down
during each mixing cycle. Range: (0-10) times.
MIX SPEED:
The speed at which the robotic arms move up and down
during mixing. Range: (1-5). (1, slowest - 5, fastest)
BASKET LIFTING SPEED:
The speed at which the instrument lifts the basket set from
the solution reservoir. Range: (1, slowest - 5, fastest)
UPS CONNECT:
This indicates whether or not the Uninterrupted Power
Supply (UPS) is connected to the instrument. Range:
Yes/No
NOTE: The UPS connect parameter needs to be set at [NO]
if there is no UPS connected to the instrument. If [YES] is
set, the instrument will enter into the power outage
operation mode regardless if a power outage actually
happened.
KEY SOUND:
The operator can select between silent operation or an
audible signal whenever a key is pressed. Range: Y es
(audible)/No (silent).
DRYER TEMPERATURE SET:

The drying station temperature, which can be input by the
numerical keys. Range: (30-65C in 1 increments)
WATER WASH CYCLE TIME:
The length of time the water flow to the wash stations
continues after the baskets(s) have left the wash station.
Range: (minimum: 30 seconds, maximum: 1 minute and 30
seconds in 1 second increments)
ENHANCED WASH FREQUENCY:
The number of times the robotic arm moves up and down
during each enhanced wash cycle. Range: (1, slowest - 5,
fastest)
Revised 3/30/2010
Error Log
The [UTILITY] function key can also be used to view any
errors that may have occurred during staining. The Error
Code Log will display a code, which is identified by a
number, and will provide a brief description of the problem.
If an error has occurred, you can access this display and
refer to the Troubleshooting Chart, beginning on page 5.1, to
review a description of the problem and a possible remedy .
The error codes can be cleared out of the log by pressing
the [CLEAR] key.
3.9
ROUTINE OPERATION
Section 4
Routine Operation
3. Open the water faucet at the sink connection to enable

the flow of water to the instrument.
This section describes the basic procedures necessary to

operate the DRS 2000. T o program a staining method or
edit an existing program, refer to Section 3, Explanation of
Software.
To manually open the wash valve to each wash reservoir,

please follow the procedure below:
Starting a Staining Process
2. Press the [SETUP] key and then the [W ASH ON] key .
The Water Flow Confirmation screen will appear.
1. Before starting a run, verify the following:

The water supply and drain hose are securely
connected
The robotic arm is free to move
The cover and the lower door are properly closed.
CAUTION: Check that the water supply hose and the
drain hose are not kinked and that all connections
are secure.
2. Turn the power switch to I position.
screen will appear.
1. Press the [MORE] key from the Main Menu to access the
second group of function keys and then press the
[UTILITY] key. The Utility Function Selection screen will
be displayed.
The Main Menu
3. The water begins flowing into the wash stations and the
water supply time begins counting when the VALVE IS
OPEN message is displayed.
4. When the water begins flowing through the rectangular
rim of the wash reservoirs, the valve can be turned of f by
pressing [CLOSE]. When the [CLOSE] key is pressed,
the valve shuts off and the water stops flowing to all wash
stations. Once the adjustment is completed, pressing the
[EXIT] key twice will again return you to the Main Menu.
TM
Tissue-Tek DRS 2000

START MONITOR
EDIT
UTILITY
STATION
MORE
MORE
Main Menu
Revised 3/30/2010
4.1
ROUTINE OPERATION
Starting a Staining Program in the
Continuous Mode
To begin a staining method in the Continuous Mode follow
the steps below:
1. Press the [ST ART] key from the Main Menu. Place the
basket set to be run in the appropriate Start Station
labeled, S1 or S2.
2. If the BATCH message is displayed on the monitor as
follows: MODE: BATCH , pressing [MODE] key will change
BATCH to CONTINUOUS and toggle between the two,
each time it is pressed. In order to run baskets is a
continuous mode, CONTINUOUS must be displayed
3. If the desired method is not currently displayed at the top
of the screen, press the [METHOD] key to view your
options. Use the arrow keys to scroll through the list of
methods then press [SELECT] to choose the program
you wish to run. T o start the desired method press the
[START] key. The robotic arm will pick up the basket set
and move it to the first programmed station.
NOTE: The [MODE] key cannot be selected while the
instrument is in operation. All baskets must be removed
before changing modes.
4.2
Starting a Staining Program in Batch Mode

To begin a method in the Batch Mode, follow the steps
below:
1. Press the [ST ART] key from the Main Menu. Place the
basket set to be run in the Start Station labeled, S1.
2. Press the [MODE] key to display: MODE: BATCH at the
bottom right side of the Start Process screen.
The robotic arm gives priority to only one basket set. When
staining in the batch mode, you may not add additional
basket sets until each run is completed. The total length of
time it will take to run one basket set in a batch mode is
completely dependent on the times programmed for that
specific protocol. If you would like to begin staining in the
batch mode of operation, you may choose a specific start
step to begin. From the Start Process screen:
1. Press the [STEP] key.
2. Use the arrow keys to scroll to the desired start step for
that run, open the door and cover, place the basket set in
the desired start step, close the door and cover and
press [START].
In batch mode operation, the stainer will accommodate up
to 40 slides/run, one basket set.
Revised 3/30/2010
Operations During Processing

Adding Basket Sets
This section describes how to add additional baskets during
staining. The [MODE: CONTINUOUS] indication must be
displayed at the bottom right side of the Start Process
screen. This indicates that the instrument is currently in the
continuous operation mode which allows the operator to
add more baskets.
To add a basket to the stainer:
1. Press the [ADD] key from the Process Monitor screen.
The run # and method will be displayed on the first line.
The message reads, PLACE THE BASKETS FOR THE 1ST
RUN AT S1 AND PRESS START. If you wish to change the
method, press the [METHOD] key and select the
desired method to be run.
NOTE: If a key is not pressed within 30 seconds, the display
automatically returns to the Process Monitor screen.
RUN:# 1 METHOD: H&E (SAKURA)
START
PLACE THE BASKETS FOR THE 1ST RUN

AT S1 AND PRESS START
MODE:CONTINUOUS
EXIT
START METHOD MODE
Monitoring a Staining Process

During the staining process, the Process Monitor screen is
displayed. The Process Monitor is a visual display which
identifies where all staining runs are located at any given
time. From the Process Monitor screen the operator can
make various selections. Additional basket sets maybe
added, end times for each run may be verified or runs may
be aborted. Pressing [MAIN] will return you to the Main
Menu where you may perform other operations.
Each basket set will be moved through the stainer according
to the specific staining method selected. A run number is
associated with each basket set and as the robotic arm
moves the basket, the run number will appear in the correct
station position displayed on the Process Monitor screen.
The Process Monitor will be continually displayed while
staining is occurring.
2
1
1
2
9 10 11 12 13
E1 E2 16 17 18 19 20 W1W2 W3 24 25 S1 S2 DRY
ADD
ENDTIME
PAUSE
REMOVE
MAIN
Process Monitor Screen
2. To add a basket, open the lower door by pressing on

the left side of the door and place the basket set in the
appropriate start station. It is not necessary to open
the cover. Close the door and press the [ST ART] key if
the desired method is already displayed. Pressing
[START] will resume operation and the display will
return to the Process Monitor screen. The process run
number of the added basket set will appear at the
appropriate station position.
Revised 3/30/2010
4.3
ROUTINE OPERATION
Interrupting or Canceling a Staining
Process
From the Process Monitor screen, the operator may choose
to interrupt or cancel a staining run. During process
interruption, the robotic arm will not move. The interruption
should be as short as possible to avoid af fecting stain
quality if multiple staining programs are in progress.
To interrupt a staining run:
1. Press [PAUSE] from the Process Monitor screen. (If the
robotic arm is moving, the message, PLEASE WAIT will
appear. When the robotic arm has stopped, the Process
Cancellation screen will be displayed.) From this screen,
the operator has three options, pressing [EXIT] will return
you to the Process Monitor screen and staining will
resume, pressing [ST ATUS] will provide an endtime
report of a specific run in progress and pressing [ABORT]
will cancel a staining run.
5
2
9 10 11 12 13
E1 E2 16 17 18 19 20 W1W2 W3 24 25 S1 S2 DRY
ABORT
STATUS
Process Cancellation Screen
4.4
EXIT
2. To obtain a status report of a specific process run, use

the arrow keys to highlight that run number and press the
[STATUS] key. A screen will be displayed indicating the
run number, staining method name, and the time left to
complete the process in hours, minutes and seconds. (If
the selected staining process has already been finished,
[00:00:00] will be displayed.) Pressing the [EXIT] key will
return you to the Process Cancellation screen.
3. To cancel a staining run, highlight the run number using
the arrow keys and press the [ABOR T] key from the
Process Cancellation screen. A display message will
appear, DISCONTINUE THE FOLLOWING RUN? With the
selected run number , station number and stain method
identified. Pressing [YES] will cancel the run highlighted.
Pressing [NO] will return you the previous screen and
[EXIT] will return you to the Process Monitor screen. The
[ABORT] key should only be used if the operator wishes
to discontinue a specific run. The arrow keys, in this
case, are used to highlight the desired run number which
will be deleted.
If a staining run was aborted, the basket set will need to be
removed from either the upper or lower level of the
instrument. The displayed message may read, PLEASE
OPEN THE DOOR AND THE COVER. The Process Monitor
screen will automatically appear after the basket has been
removed for confirmation of removal. V erify that the cursor
highlights the correct station position and press the
[CONFIRM] key. The display will return again to the
Process Cancellation screen or to the Main Menu if there
are no other baskets currently staining.
Revised 3/30/2010
Checking the Endtime for a Staining Run

in Progress
While a staining run is in progress, the operator can check
the time left for that run and get an estimated time for
completion. By pressing the [ENDTIME] key from the
process monitor, the screen will display the run number for
each program currently running and the estimated time
remaining to complete the process in hours, minutes, and
seconds. The staining step, position, and the time left for
completion in that specific station is also displayed.
TIME REMAINING TO END
RUN TIME LEFT
1 00:31:28
2 00:42:27
3 00:53:27
UPDATE
STP
3
2
1
STA
2
1
S1
TIME LEFT
00:01:57
00:02:24
00:02:46
EXIT
Endtime Screen
NOTE: The clock is not a real-time clock. Y ou may press
the [UPDATE] key which will update the times left for each
run.
The END OF RUN audible tone is triggered once a run is

completed and has entered either the PE station or one of
the end stations. The alarm will beep for ten seconds and
continues every 30 seconds until the basket is removed.
To change the alarm to a single beep, please perform the
following steps:
1. If the instrument is not operation turn the power to the
OFF position.
2. Press and hold the 0 key while turning the power ON.
3. Select 1. PROPERTIES
4. Select 4. MISCELLANEOUS
5. Select [CONTINUOUS] or [SINGLE] by using the arrow
keys; confirm by pressing [MEMORY].
6. Turn the power to the OFF position. In the continuous
mode, the alarm will beep for 10 seconds in 30 second
intervals. The single mode will alarm for 10 seconds and
stop. It is necessary to remove the basket(s) when the
stain process has ended.
End of a Staining Process

When a staining process is completed, the message THE
STAIN PROCESS COMPLETED. PLEASE REMOVE THE BASKET
FROM E1, E2, OR PE is displayed and an audible tone is
triggered. The Process Monitor screen will automatically

appear after 15 seconds. The End Station, (E1) and or (E2)
or (PE) is highlighted.
1. Remove the basket set from the (E1), (E2), or (PE) End
Station. You will need to press the [CONFIRM] key to
verify removal of the basket set.
If there are other staining programs in progress, the display
returns to the Process Monitor screen and the stainer
resumes operation of remaining runs. If no other staining
programs are in process, the display will return to the Main
Menu screen.
Revised 3/30/2010
4.5
TROUBLESHOOTING
Section 5
General Information
This section helps the operator identify instrument
malfunctions, their probable causes, and corrective actions
that can be taken to remedy the problem. If the malfunction
listed in the troubleshooting section is observed, follow the
instructions given in the REMEDY column. If you have
difficulties in resolving the problem or if the problem is
beyond the scope of this manual, contact Sakura Finetek
for technical support.
The following T roubleshooting Chart lists the warning
messages and error codes that could occur during
operation of the Tissue-Tek DRS 2000 Automatic Slide
Stainer. Possible problems could be electrical, mechanical,
or operational. Probable causes and recommended
remedies are also included so that many isolated problems
can be quickly corrected.
If additional assistance is required concerning an
instrument problem, or if the problem cannot be isolated or
is beyond the scope of this manual, please contact Sakura
Finetek U.S.A. for technical support. Y ou can reach the
Customer/Product Support Department by calling toll free 1800-725-8723, option 2 (U.S. only). If located outside the
U.S., contact the nearest T issue-Tek distributor or
representative for information and assistance.
Revised 3/30/2010
5.1
TROUBLESHOOTING
TROUBLESHOOTING CHART
PROBLEM
POSSIBLE CAUSE
REMEDY
If a UPS is connected, the instrument has

received a power failure signal from it.
Power Out Signal
The instrument will continue operation for five

minutes since the UPS supplies back-up power;
however, no heat will be supplied to the drying
station. If the power failure lasts for over five
minutes, the instrument will stop. If the UPS is
connected, wait until the power is restored. If the
UPS is not connected, the [UPS Connect]
parameter needs to be changed to [No] in the
Default Parameter screen.
Display does not light up. Instrument does not

operate.
Power switch is off.

Power cord is not plugged in.
Defective power source.
Move the switch to the I position.

Plug the cord into power source.
Check the laboratory breaker or receptacle.
Display is too dark or too bright.
Brightness of display is not properly adjusted.
Adjust the display to the desired brightness

level. Use the brightness adjustment dial on the
front, right side of the instrument above the
power switch.
Staining process does not start. (Robotic arm

does not move.)
Cover and/or lower door is open or not properly

closed.
Error Message [Power Out Signal] is displayed.
Close the cover and/or the lower door

completely.
If the UPS is connected, power failure has
occurred. Staining cannot begin until power is
restored. If the UPS is not connected, the [UPS
Connect] default is set to [YES]. Change the
[UPS Connect] default value to [NO].
Robotic arm suddenly stopped and an audible

alarm sounded.
Robotic arm hit an obstacle.

Solution reservoirs are not properly installed.
Remove or adjust the obstacle.

Level solution reservoirs and place them in the
proper position.
Solution or water leaks from the instrument.
The drain hose is occluded or bent.

The drain hose is higher than the drain port of
the instrument.
The drain hose is twisted or looped.
Water supply stainer connection is not properly
sealed.
Remove any bends or kinks in the hose.

Place the hose lower than the drain port, and
position it straight.
Wash reservoir does not fill to the proper level.

Water flow is not sufficient.
Defective rubber grommet.

The water faucet is closed.
The water supply adjustment valve is closed.
Replace the grommet. (Product code 4985)

Open the water faucet.
Open the water supply adjustment valve.
Water flow is not sufficient.
Low water supply pressure.
Check the water supply pressure, 0.098 to 0.441

MPa (1.0 to 4.5 kgf/cm2) is required for
adequate water supply pressure, (dynamic
pressure).
Clean filter screen.
Clogged screen filter in supply hose.
5.2
Tighten the water supply strainer. If solution still

leaks, the strainer may have a defective gasket.
Contact the Sakura Customer/Product Support
Department.
Revised 3/30/2010
ERROR
CODE
DESCRIPTION
POSSIBLE CAUSE
REMEDY
SYSTEM ERROR
A control error has occurred. The

instrument will immediately stop
operation.
Turn off instrument power. Call and inform the Sakura

Customer/Product Support Department.
POWER FAILURE
The power supply was shut down due

to a power failure or the power cord
was disconnected.
When power is restored, the robotic arm will move to its home position and
the operation will stop. Follow these steps:
1. (Over station 27) P ower failed for only a short period of time, the
operator can decide to continue the staining process at the point
where the interruption occurred.
2. To continue the process where the instrument was interrupted,
press the [RESUME] key. The staining process will resume.
3. To cancel the process, press the [EXIT] key. To confirm, press
[YES] or [NO], then the All Basket Removal Confirmation screen
will appear. Remove all the basket sets from the instrument, and
press the [EXIT] key. The robotic arm will move to the center of the
instrument and the Main Menu screen will be displayed.
3, 5, 6
MEMORY ERROR
The instrument memory has been

corrupted. The instrument will
immediately stop operation. Any of
Error Codes 3, 5, or 6 will appear in
the upper right hand corner of the
display which relate to the cause of
the memory malfunctions.
Service is required. Call and inform the Sakura Customer/Product

Support Department.
BATTERY LOW
The voltage of the memory back-up is

low.
The battery will need to be changed. Call Sakura Customer/Product

Support. Although the instrument will continue operation, failure to
replace the battery may induce a memory loss when the instrument is
turned off or if power failure occurs. Pressing the [EXIT] key will allow
operation to resume.
Revised 3/30/2010
5.3
TROUBLESHOOTING
ERROR
CODE
11-15
DESCRIPTION
ROBOTIC ARM
MALFUNCTION
POSSIBLE CAUSE
The robotic arm does not operate as

expected.
REMEDY
The instrument will immediately stop. An error code from Code 11 to

15 will appear in the upper right hand corner of the display according
to which sensor has detected a failure. Follow the steps below:
1. Open the lower door (and the cover, if needed). Look inside the
instrument for an obstacle to the robotic arm movement and
remove it if necessary. Close the cover and lower door and press
the [RESUME] key. After the instrument performs the recovery
operation, the staining process will resume.
2. If the same error message occurs again, press the [EXIT] key. The
All Basket Removal Confirmation screen will appear. Remove all
the basket sets from the instrument and press the [EXIT] key. The
robotic arm will move to the center of the instrument and the Main
Menu screen will be displayed.
3. If the error message does not disappear, the driving mechanism or
the position detection mechanism of the robotic arm may possibly
be at fault. Call and inform the Sakura Customer/Product Support
Department.
30
SENSOR OPEN
The thermal sensor for the drying

station does not function.
The instrument will operate normally except that no heat will be

applied to the drying station. Call and inform the Sakura
Customer/Product Support Department.
31
SENSOR SHORT
The thermal sensor for the drying

station is short circuited.
Call and inform the Sakura Customer/Product Support Department.
32
TEMPERATURE
LOW
The drying station cannot reach the

set temperature.
33
TEMPERATURE
HIGH
The drying station temperature has

exceeded the set value.
34
DRY FAN STOPPED
The drying station fan has stopped

operating.
The instrument will operate normally except that no heat will be

applied to the drying station. Repair will be required. Call and inform
the Sakura Customer/Product Support Department.
50
FUME FAN
STOPPED
The ventilation fan has stopped.
The instrument will continue operation except that the fan will not run
Since there is no ventilation inside the instrument, service is required.
Call the Sakura Customer/Product Support Department.
5.4
Revised 3/30/2010
CARE OF THE INSTRUMENT

General Maintenance
Keep the exterior of the DRS 2000 free from dust. Do not
use solvents of any kind on the front of the control panel;
however, if disinfection of the keypad is desired, 70% to 85%
ethanol or isopropanol can be wiped on and allowed to air
dry. The plastic cover may be cleaned with a glass cleaner
and soft cloth.
Daily Maintenance
Exterior/Interior Surfaces
Daily clean the control panel, plastic, and painted areas by
wiping with a clean cloth. Clean the interior of the solution
area by wiping with a clean cloth.
Bi-monthly Maintenance
Replacement of the Activated Carbon Filter
It is recommended that the activated carbon filter be
changed after two weeks or sooner if fume monitoring
reveals elevated reagent vapors.
Keep the filter in place even if there is an internal duct
system attached.
To replace the activated carbon filter , follow these
instructions:
1. Turn the power of f by pressing the O side of the power
switch.
2. Open the lower door first and then the cover and remove
all the solution reservoirs in the lower section of the
stainer.
NOTE: Label the reservoirs so that you can place them
back in their original positions.
3. Pull each wash reservoir gently toward you to drain any
water in the reservoir then remove all wash reservoirs.
4. Move the robotic arm to the right side of the instrument
with enough care to prevent it hitting on the solution
reservoirs in the upper section.
Revised 3/30/2010
Section 6
5. Release the filter cover by pressing on the black cover

button while holding the filter cover with your left hand,
slide the filter cover slightly to the left and remove it.
6. Remove the activated carbon filter from the instrument
and dispose of it properly.
7. Unpack the new activated carbon filter and reinstall the
filter gasket and the new filter.
8. Hang the left end of the filter cover on the cover hook of
the instrument and lock the filter cover by pressing on the
black cover button.
9. Return all reservoirs to their original positions, then close
the cover and the lower door.
Monthly Maintenance
Drying Station
The drying station should be cleaned once a month or more
often as needed.
1. Turn the power of f by pressing the O side of the power
switch. The power switch is located on the front side of the
instrument to the right of the lower door . Open the lower
door first and then the cover. Wait until the temperature of
the drying station has reached a comfortable level, then
take out the inner lining of the drying station.
2. Remove the residue from the inner lining by washing with
water and air drying. Return the inner lining to the inside
of the drying station and verify that the raised portion of
the liner is facing the right hand side of the drying
compartment.
Solution Reservoirs and Slide Baskets

The solution reservoirs and slide baskets should be washed
with tap water or a mild detergent once a month. If you have
difficulty in removing stains and residue from dried reagents,
soak first in a commercially available chemical cleaning
detergent or bleach solution and then rinse with a mild
detergent and water . Cleaning reservoirs and slide baskets
monthly will help keep these items free from permanent
stains. Always use the same reservoir for a given solution to
avoid contamination.
6.1
CARE OF INSTRUMENT
Reservoir Trays
The reservoir trays should be cleaned once a month. T urn
power off by pressing the O side of the power switch.
NOTE: If the robotic arm is interfering with the cleaning
procedure gently move the robotic arm out of the way , only
if the power is off.
Open the lower door first and then the cover . Carefully
remove all the solution reservoirs from the instrument.
NOTE: Be sure to label the reservoirs in order to put them
back in their original position once the cleaning procedure is
complete.
Pull each wash reservoir gently toward you to drain out the
water and then remove all solution reservoirs from the lower
section. The lower reservoir tray can be removed by gently
lifting the front edge with both hands and sliding the tray
away from the front of the instrument. Remove the left and
right reservoir trays from the upper section. Clean the
reservoir trays and the inside of the upper and lower
compartments by wiping with a clean wet cloth. Reinstall the
reservoir trays in the upper and lower compartments. The
lower reservoir tray can be reinstalled by gently placing the
tray under the wash nozzles and sliding the tray toward the
front of the instrument. Return the solution reservoirs and the
wash reservoirs to their original positions. Close the cover
first and then the lower door.
2. To reattach the strainer , turn it clockwise and tighten it by

using your hand only. Fit the drain receiver into the drain
port of the reducing valve. At this time, the fastener has to
be held in an open position. T urn the fastener 90
clockwise to lock the drain receiver . Open the water
faucet and turn the instrument on.
CAUTION: Failure to close the external water supply
faucet before attempting to clean the strainer may
cause spillage.
NOTE: For a more thorough cleaning, use an ultrasonic
bath if available. Attach the strainer by turning it clockwise
and tighten it by using your hand only.
NOTE: If there is water a leak at the strainer connection,
tighten it using a wrench. If the leak is still present,
contact the Sakura Customer/Product Support
Department as the sealing gasket may be damaged.
Water Supply Strainer

The water supply strainer should be cleaned once a month.
You will need a toothbrush and a wrench to perform this
procedure.
1. Close the external water supply faucet. Failure to close
the water faucet before attempting to clean the strainer
may cause spillage. Turn the power off by pressing on the
O side of the power switch. T urn the fastener 90
counterclockwise to release the drain receiver. Pull out the
drain receiver toward you. Using a wrench if necessary ,
remove the strainer by turning it counterclockwise. Use a
cloth to avoid spilling water from the strainer . Wash the
mesh portion of the strainer in water using a toothbrush to
dislodge particles.
6.2
Revised 3/30/2010

Service Information
When you have a problem with the
instrument
When problems arise during operation of the T issue-Tek
DRS 2000 Automatic Slide Stainer , first refer to Section 5,
Troubleshooting. A void problems by carefully following
proper operating and cleaning procedures. If the problem
cannot be solved and an instrument failure is apparent, our
Technical Support Staff is available to assist you.
Section 7
Where to Call for Service

If located within the United States, contact the
Customer/Product Support Department of Sakura Finetek
U.S.A., Inc. by calling toll free:
1-800-725-8723, option 2
In countries other than the United States, contact the
nearest authorized T issue-Tek instrument distributor or
representative for service information and assistance.
Before calling for technical support, note the instruments

serial number and any error codes. Be sure that you know
the details of the problem you are experiencing. This
information will help the T echnical Specialist to identify the
probable cause of your instrument malfunction.
Revised 3/30/2010
7.1

Accessories and
Replacement Parts
Please use the following product codes to order
replacement accessories. Replacement of the battery should
be done by a qualified service technician only
CAUTION: When replacing parts, only use the
recommended parts. The use of non-compatible parts
may cause instrument malfunction.
Accessories
Description
Product Code
Quantity
Activated Carbon
Filter
2008
2/case
Water Supply Hose
2208
Each
Slide Basket
4768
10/case
Solution Reservoir
4974
Each
Wash Reservoir
4975
Each
Reservoir Lid
4976
Each
Basket Hook
4977
Each
Basket Adapter
4978
Each
Wash Station Plug
4979
Each
Drain Hose
4981
Each
Wash Reservoir
Grommet
4985
Each
Six- Reservoir Lid
4986
Each
Seven- Reservoir Lid
4993
Each
Qualified Service Technician Replacement Parts

Description
Product Code
Quantity
Battery Unit
F51-074-00
Each
7.2
Revised 3/30/2010
STAINING RECORD
Appendix A
Sample Staining Record

On the following page is an example page for keeping a
record of your staining programs and progress. It is highly
recommended that you keep records of your individual
programs and notes. Make copies of the blank staining
record for your laboratory use.
The page for recording staining progress is intended only as
a guide; modify it as necessary to satisfy the individual needs
of your laboratory.
Revised 3/30/2010
A.1
A.2
Revised 3/30/2010
Staining Record
Revised 3/30/2010
A.3

Operating Manual DRS2000 (ENG)

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Tissue-Tek DRS 2000

Automatic Slide Stainer

20 Sakura Finetek USA, Inc.

EXPLANATION OF SOFTWARE AND CUSTOMIZATION OF SETTINGS

SERVICE AND REPLACEMENT PARTS

The DRS 2000 consists of four major components:

The Tissue-Tek DRS 2000 Automatic Slide Stainer is

the control panel, through which all operations are

Control Panel (Figure 1-2)

Reagent Reservoir Compartment (Figure 1-3)

The reagent reservoirs are arranged on a two-level design.

Robotic Arm (Figure 1-4)

Fume Control (Figure 1-5)

The activated carbon filter is part of an ef fective fume

The DRS 2000 has four adjustable feet  at each corner of

Power Switch (Figure 1-6, Figure 1-7)

Brightness/Contrast Dial (Figure 1-6, Figure 1-7)

This dial  controls the brightness and the contrast of the

External Connection Terminals (Figure 1-8)

Rear of Instrument (Figure 1-9, Figure 1-10)

The solution, wash, start, and end stations can be

In a continuous mode of operation, the user has the

Staining time for that step will be performed

Staining time may be extended to some

Infinite [ ]: Staining time for that step may be extended

The DRS 2000 is designed to ef fectively remove fumes

Up to 20 programs can be stored in memory

Up to 100 solution names can be stored in memory

Staining Method Names:

This section provides information on selecting a proper

1. Unpack the instrument by removing the large nails along

The DRS 2000 is a precision instrument and must be

CAUTION: The instrument is very heavy and large;

If any of these items are missing, contact your

Drain Elbow Assembly

Activated Carbon Filter Cartridge -1

Water Supply Hose Set -1

Filter Gasket for Carbon Cartridge - 1

Reagent Trays (for upper level) - 2

Drain Hose Clamp - 1

Reagent Tray (for lower level) - 1

CAUTION: The robotic arm is secured at the lower left

3. To install the drain hose, place the drain hose assembly

CAUTION: Make sure that the entire length of the

2. Before attaching the water supply hose to the

4. In order for water to begin flowing to the wash reservoirs,

8. The solution reservoirs may be placed in the upper and

10. If the power cord is not already attached, plug the

Tissue-Tek DRS 2000

Main Menu Screen

External Connection Terminals

2. Connection to an External Alarm Device

When the instrument detects a power failure signal

The instrument can transmit the following error signals to

The instrument will continue normal operation for 5

If power is restored within 5 minutes, the instrument

If the power failure continues over 5 minutes, the

Status of output terminal circuit:

Error Output Conditions

3. Duct Connection Adapter (optional)

20 Sakura Finetek USA, Inc.

The DRS 2000 has four adjustable feet at each corner of

This dial controls the brightness and the contrast of the