Entrance Test Syllabus For M. Tech (Pharma. Biotechnology) (ICT Admission 2016-17)

Institute of Chemical Technology, Matunga, Mumbai
Syllabus for M. Tech. Pharmaceutical Biotechnology Entrance Examination

Dept. of Pharmaceutical Sciences and Technology, 2016-17
Microbiology: Microbial Taxonomy and Diversity; Eukaryotic microorganisms;
Viruses; Structure and Functions of Prokaryotic and Eukaryotic Cells; Microbial growth
and growth curve, introduction to immunology, staining and microscopes, sterilization
Biochemistry: Study of carbohydrates, Glycolysis, Gluconeogenesis, Glycogen
synthesis, Krebs cycle, Study of Lipids, Proteins & Amino acids, Nucleic acids and their
components, Enzymes- definition, function, nomenclature, classification, mechanism
of enzyme action, specificity of enzymes, enzyme kinetics, enzyme inhibition and
regulation, Methods and applications of immobilization of cells and enzymes, Vitamins
& Co-enzymes, Concept of free energy, standard free energy vs transformed free
energy vs free energy for a reaction. Relationship of standard free energy to reaction
equilibrium constant, concepts of enthalpy and entropy, introduction to first and
second law of thermodynamics. Standard free energy changes of some important
biological rections. Concept of oxidation reduction reactions, standard electrode
potential, transformed standard electrode potential, standard electrode potentials of
some biological important redox couples.Concept of high energy phosphate bond and
ATP as a carrier of energy. Electron transport chain: Components of the ETC,
oxidative phosphorylation vs substrate level phosphorylation. Discussion or proton
motive force and generation of ATP by use of proton gradients. Examples of some
toxins that interfere with ETC.
Molecular Biology: Recombinant DNA technology; prokaryotic and eukaryotic
expression systems; Vectors; Gene mutation: Types and mutagens; Bacterial, yeast,
genetic system: transformation, conjugation, transduction, recombination; techniques
in molecular biology like PCR and RT-PCR, microarray technology, DNA fingerprinting
Fermentation and bioreactors: Principles of surface and solid state fermentation,
Design of different fermentors and the biochemical engineering aspects. Process
control of fermentations. Fermentation technology of industrial chemicals, organic
acids, amino acids, vitamins, polysaccharides, antibiotics, etc. Enzyme fermentation
and technology including immobilization and enzyme reactors. Fermentative animals,
and other developments
Tissue culture (plant and mammalian): Nutritional requirements for animal and plant
cell culture; techniques for mass culture of animal cell lines; application of plant and
animal cell culture. Use of plant/animal cell culture for production of pharmaceuticals,
hybridoma technology and gene knockout; stem cells and its application in organ
synthesis; gene therapy; transgenic animals
Biologicals and Biopharmaceuticals: Antibodies: polyclonal and monoclonal
antibodies, catalytic antibodies, diagnostic antibodies; production of antibodies,
vaccines: types, production and applications; Therapeutic proteins and peptides:
Insulin, erythropoietin, interleukins, hormones, sterilization methods for
biopharmaceuticals.
Analytical methods for biologicals and biopharmaceuticals: Absorption
spectroscopy (UV, Visible and IR), fluorimetry, conductometry, potentiometery,
principles of mass, NMR, Thermal analysis, dynamic light scattering techniques, AFM,
SEM, TEM, EDX, X-ray diffraction and analytical chromatographic methods; Principles
and methods of microbial assay of pharmacopoeia, filter integrity test, sterility testing
of biopharmaceuticals, methods of analysis of for proteins. , Gel electrophoresis

techniques (PAGE, SDS-PAGE and agarose gel electrophoresis), immunoblotting,
Southern blotting, Northern blotting, Western blotting, PCR and RT PCR, Sanger
dideoxy method of sequencing, RIA and ELISA diagnostic methods
Applications of Biotechnology in Pharmaceutical Technology, food and
nutrition: Use of biologicals and biotechnological processes to make food and
pharma products including microbial and enzymatic biotransformations; Biological
sciences involved in action of bioactives in health care in food and pharma products.
Production of monoclonal and polyclonal antibodies, hormones, protein and peptides
etc.

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Institute of Chemical Technology, Matunga, Mumbai

Syllabus for M. Tech. Pharmaceutical Biotechnology Entrance Examination

of biopharmaceuticals, methods of analysis of for proteins. , Gel electrophoresis

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