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    CH 20 Guided Reading

    Uploaded by

    Sylvia Grace
    This document contains a guided reading assignment on chapter 20 of an AP Biology textbook. It includes 43 questions about key concepts in gene cloning, DNA hybridization, polymerase chain reaction, gel electrophoresis, genetic mapping, DNA sequencing, genomics, and applications of biotechnology like gene therapy and genetically modified foods. Students are asked to define terms, label diagrams, explain processes, and discuss concepts covered in the chapter.

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    © All Rights Reserved
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    CH 20 Guided Reading

    Uploaded by

    Sylvia Grace
    350 views9 pages
    This document contains a guided reading assignment on chapter 20 of an AP Biology textbook. It includes 43 questions about key concepts in gene cloning, DNA hybridization, polymerase chain reaction, gel electrophoresis, genetic mapping, DNA sequencing, genomics, and applications of biotechnology like gene therapy and genetically modified foods. Students are asked to define terms, label diagrams, explain processes, and discuss concepts covered in the chapter.

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    This document contains a guided reading assignment on chapter 20 of an AP Biology textbook. It includes 43 questions about key concepts in gene cloning, DNA hybridization, polymerase chain reaction, gel electrophoresis, genetic mapping, DNA sequencing, genomics, and applications of biotechnology like gene therapy and genetically modified foods. Students are asked to define terms, label diagrams, explain processes, and discuss concepts covered in the chapter.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    350 views9 pages

    CH 20 Guided Reading

    Uploaded by

    Sylvia Grace
    This document contains a guided reading assignment on chapter 20 of an AP Biology textbook. It includes 43 questions about key concepts in gene cloning, DNA hybridization, polymerase chain reaction, gel electrophoresis, genetic mapping, DNA sequencing, genomics, and applications of biotechnology like gene therapy and genetically modified foods. Students are asked to define terms, label diagrams, explain processes, and discuss concepts covered in the chapter.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 9

    Adapted from L. Miriello by S.

    Sharp

    AP Biology Name _________________________


    Chapter 20 Guided Reading Assignment

    1. Define the following


    a. Recombinant DNA

    b. Genetic engineering

    c. Biotechnology

    d. Gene cloning

    2. Why did restriction enzymes evolve in bacteria?

    3. Define the following terms


    a. Restriction site

    b. Restriction fragments

    c. Sticky end
    4. Label the following diagram.

    5. Using the diagram below label the steps to cloning a human gene in a bacterial
    plasmid

    Page 1 of 9
    Adapted from L. Miriello by S. Sharp

    6. Explain in your own words two ways that we know the cell clones carry the recombinant
    plasmids?

    7. What is the purpose of nucleic acid hybridization? Why is the word hybrid used?

    8. What is a complementary, short, single stranded nucleic acid that can be either DNA or
    RNA called?

    9. Why do scientists use a radioactive isotope tag for the probes?

    Page 2 of 9
    Adapted from L. Miriello by S. Sharp

    10. How is DNA denaturation different than protein denaturation?

    11. Label the following steps of nucleic acid probe hybridization.

    12. Define genomic library.

    13. How are bacteriophages used for making genomic libraries and what are some of
    the advantages of this?

    14. What are the steps in making complementary DNA cDNA?

    15. Compare and contrast the advantages of cDNA libraries and genomic libraries.

    16. What is an expression vector and what problem does it solve?

    17. Why do molecular biologists use yeast as opposed to bacteria for expressing
    genes of interest?

    18. Define yeast artificial chromosomes.

    Page 3 of 9
    Adapted from L. Miriello by S. Sharp

    19. What is electroporation?

    20. Why is PCR polymerase chain reaction important in many aspects of


    biotechnology?

    21. Label the diagram of PCR below.

    22. What is the purpose and general process of gel electrophoresis?

    Page 4 of 9
    Adapted from L. Miriello by S. Sharp

    23. Label the diagram below focus on the charge, molecule size and results.

    Page 5 of 9
    Adapted from L. Miriello by S. Sharp

    24. Label the diagram below focus on identifying the fragments.

    25. Define and explain the significance of RFLPs restriction length polymorphisms.

    26. What was the purpose of the Human Genome Project?

    27. Label the diagram outlining the Southern Blotting of DNA Fragments

    Page 6 of 9
    Adapted from L. Miriello by S. Sharp

    28. Why is genetic mapping considered a relative mapping as opposed to physical


    mapping?

    29. What is the goal of DNA sequencing?

    30. Outline the diagram below of Dideoxy Chain Termination I know this seems
    difficult to follow at first but at least copy the main ideas before we go over it in class.

    31. What is the basic concept of the whole-genome shotgun approach to


    sequencing?

    Page 7 of 9
    Adapted from L. Miriello by S. Sharp

    32. Define genomics.

    33. Is there a direct correlation between size of the genome and the complexity of
    the organism?

    34. What is in vitro mutagenesis and what does it help the scientist understand?

    35. What is proteomics?

    36. Define single nucleotide polymorphisms.

    37. What are some of the examples of the medical applications of biotechnology?

    38. What are the basic steps in human gene therapy with a retroviral vector?

    39. Have there been problems with this procedure?

    40. What is a DNA fingerprint?

    41. What is a transgenic animal?

    42. How are plasmids used in agriculture and genetic engineering in plants?

    43. What are genetically modified foods and do you think that you have eaten any?

    Page 8 of 9
    Adapted from L. Miriello by S. Sharp

    Page 9 of 9

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