Microplate Reader: M.R.C LTD
Microplate Reader: M.R.C LTD
Microplate Reader: M.R.C LTD
UT-6100
Microplate Reader
M.R.C LTD.
OFFICES: HAGAVISH 3, HOLON 58817
P.O.B. 111, HOLON 58100, ISRAEL
TEL: 972-3-5593105,972-3-5595252 FAX: 972-3-5594529
Users Manual
In order to obtain the best result, it is important for you to get familiar with our instruments
This User's Manual is the operation instructions for Microplate, which including the contents
of instrument installation, daily use, and maintenance etc.
Please reserve all the packing materials for the use of deposit, transportation and
Notes: Tips, advices and suggestions will be presented in italics for distinction.
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This symbol means that the labeled item is hot while the instrument is in use.
Dont touch the labeled item as you could be burnt.
The symbol is labeled on the lamp support of optic system.
This means that the labeled item could lead to personal injury and/or damage
to the analyzer.
The symbol is labeled beside the power outlet and some external interface.
The symbols for SERIAL NUMBER, The serial number shall be after or
below the symbol, adjacent to it.
The symbol indicates the manufacturer and its address, after which are shown
its name and address.
The symbol indicates the date of manufacture, after which are shown its
manufacture date.
The symbol indicates biological pollution, marked in the part where the
instrument contacts the clinical reagent. The symbol appears in black side
and yellow background.
This means that instrument should handle with care in the course of
transportation, so as not to damage it.
The symbol means the instrument packaged should not be upended at any
time
The symbol means that the level piled up can't exceed 8 layers, as not to
damage instrument.
The symbol indicates temperature range of the analyzers during storage and
transportation.
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This instrument is only used for in vitro diagnosis, please carefully read the following
warnings before the usage, and strictly obeys them.
z The articles and materials used in some test items have biological dangers.
While operating the instrument, protective suits and glasses must be put on, and
the protective casing must be put down (enzyme label cover).
z Please dont discard the used medical materials as you pleases, it should be
dealed with according to the medical wastes.
z Pharmaceutical affairs law and regulation: It is forbidden to refit the medical
instruments.
z This instrument connects the earth by the groundwire of power line, and the
groundline must be connected to the earth to avoid electrical shock.
z Power supply must be stable. It is prohibited to use power source with high-power
equipments together.
z In the area with unstable voltage, please use UPS power supply to ensure the
normal work of the instrument.
z When power line is pulled out, you must hold plug itself, not the power line.
z Turn off the power supply immediately and contact the seller if the instrument
gives out smog, peculiar smell or strange sound.
z After finishing test work, analyzer must be turned off according to the standard
procedures, in order to avoid data loss.
z Power supply must be cut off and the mainframe must be turned off while
maintaining or disassembling the external shells due to other reasons.
z When the instrument is turned on and off, the switch key must be ensured to
immediately block to the very position, so as to prevent system damages.
z Please put on rubber gloves while maintaining and protecting, please wash hands
after the work of each time to avoid virus infections.
z Dont let your hands or other parts of your body contact with the reagent or patient
samples. If the wounded parts touch the patient samples uncarefullyplease wash
and accept doctor checkups in time.
z When the enzyme plate is used to incubation, water under the plate must be
cleaned. Otherwise, the internal wares of the instrument may be eroded, resulting
in the inaccuracy of test results.
z The product is a bench-type testing device, without issues on taboo.
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CONTENT
2. INSTRUMENT INSTALLATION................................................. 11
2.1. Instrument unpacking .................................................................................11
2.2. Environment requirement ...........................................................................11
2.3. Power source requirement..........................................................................11
2.4. Begin installation........................................................................................ 12
2.4.1. Connect instrument to power supply............................................................................ 12
2.4.2. Connect external printer................................................................................................. 12
2.4.3. Connect instrument to computer................................................................................... 12
4. INFORMATION MANAGEMENT...............................................15
4.1. Patient information..................................................................................... 15
4.2. User management ..................................................................................... 15
4.3. Basic settings............................................................................................. 16
4.4. Department management .......................................................................... 16
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6. SAMPLE TEST..........................................................................24
6.1. Template type ............................................................................................ 24
6.2. Test mode and Calculation method............................................................ 24
6.3. Set plate and plate moving settings ........................................................... 24
6.3.1. Set plate parameter settings.......................................................................................... 24
6.3.2. Plate moving parameter settings .................................................................................. 25
6.4. Select test item .......................................................................................... 25
6.5. Set plate .................................................................................................... 25
6.5.1. Sample.............................................................................................................................. 25
6.5.2. Blank ................................................................................................................................. 25
6.5.3. Negative control............................................................................................................... 26
6.5.4. Positive control ................................................................................................................ 26
6.5.5. Threshold serum ............................................................................................................. 26
6.5.6. Standard ........................................................................................................................... 26
6.5.7. Quality control.................................................................................................................. 26
6.5.8. Clear.................................................................................................................................. 26
6.6. Clear whole board...................................................................................... 27
6.7. Test ............................................................................................................ 27
6.8. Absorbance display ................................................................................... 27
6.9. Quantitative result display.......................................................................... 27
6.10. Qualitative result display ............................................................................ 27
6.11. Print ........................................................................................................... 27
6.12. Save .......................................................................................................... 27
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Microplate cover: It can prevent dusts from entering the Microplate and the interior
of instrument, Microplate, the moving work platform and rails are under it.
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Power supply indicator light: After the instrument is on, this indicating lamp is
lightened.
Running indicator light: When the instrument is running, this indicating lamp is
lightened.
Warning indicator light: When the instrument warns, this indicating lamp is lightened.
Indicator light of light path power supply: When halogen lamp is on, this indicating
lamp is lightened.
Fuse: If there is still no power supply after turn on power switch, check the fuse of
instrument.
Hinge: It is used to connect the bottom shell of instrument and the upper cover.
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2. Instrument installation
Unsealing the instruments package, and remove the materials in transportation. Preserve the
packing box and materials well. Its convenient for you to repack the instrument someday in
the future.
Find a place without direct sunshine in your working place. The surface of the selected
working table should be flat, and there is enough space to place Microplate Reader and PC
computer. The anterior edge of instrument should be near to the edge of working table. Avoid
comparatively large shake of working table (For example, put hydroextractor on the working
table).
In order to guarantee the normal working of instrument, the following places are forbidden to
place the instrument:
z Places with extreme changes of temperature.
z Particularly hot or cold places.
z Places full of dust.
z Places nearer to the electromagnetic devices which produces magnetic field.
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1) Insert one end of the power line into the outlet of instrument.
2) Insert the other end of the power line into the outlet of power supply.
Warning:
z Power supply must be well earthed.grounding voltage<5V.
z Power supply must be stable. It is prohibited to use power supply with
high-power equipments together.
z When power line is pulled out, you must hold plug itself, not the power
line.
z Turn off the power supply immediately and contact the seller if the
instrument gives out smog, peculiar smell or strange sound.
This system is compatible with most types of USB Interfaces and parallel Interface printers.
Please connect printer to the computer connected with this instrument according to the
printer users manual, and install.
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3. System log-in
If the user has already inputted the serial number, this dialogue box will not appear the next
time.
Inputting serial numbers enable us providing maintenance service of instrument to users
rapidly.
.
Figure 3-2 User log-in
Select user name in the list frame, input password in the password frame, and click OK
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button. If the user name and password are correct, it will enter system self-checking,
otherwise, the system will indicate the failure of user log-in. Click exit button to exit this
system.
There are two kinds or authority of a user, administrator and common user.
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4. Information management
Including the patient's basic information input, the form information input, querydelete and
add the functions of the items by the patients.
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Reagent information includes the functions of reagent adding, deleting and revising, etc. As
shown in Figure 4-5.
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Click Item Set under Item in the main menu, or click Item Set on the toolbar, enter item
settings menu. As shown in Figure 5-1.
In this menu, items can be added, revised and deleted. The attribute of item mainly includes
basic information of items, standard settings, quality control settings and the selection of
effective formula.
100 items are permitted to be set at the most. The items set by users are displayed in the list
on the left side of the window.
5.1. Methodologies
There are three kinds of methodologies, endpoint, two points and Kinetics.
Microplate Reader supports many kinds of different calculation methods, and there are 3
types as following.
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coefficients, and they are inputted by users according to the reagent instructions. The
qualitative formula of other forms can be changed to this pattern. For example: when sample
OD/Negative control OD2.1, its positive, and X=2.1, Y=0, Z = 0, Fac=0.
The result of qualitative calculation is manifested as the retio of sample absorbance and
Cut-Off threshold, and the unit is s/co.
1) Single point calibration: One standard needs to be set, take the line between the original
point and standard point as calibration curve (X-axis is concentration, and Y-axis is
absorbance, the same with the following). Single point calibration must set blank to
adjust the zero point. As shown in Figure 5-2.
Absorbance
Concentration
2) Non-linear regression (point to point): 2-8 standard preparations are permitted to set,
take the lines between all the standard points (it should be a monotonous ascending or
descending polyline) as calibration curve, as shown in Figure 5-3.
Absorbance Absorbance
Concentration Concentration
Concentration
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3) Multiple-point percentage %ABS: 2-8 standard preparations are permitted to set, the
biggest absorbance value is defined as 100% among the standard preparations, and the
absorbances of other standards and samples to be test are manifested by the
percentage to it. The calibration method is the same with that of non-linear regression.
4) Linear regression: 2-8 standards are permitted to set. Through these standard points, a
straight line is regressed, Y = kX + b as the calibration curve, as shown in Figure 5-5.
Absorbance
Concentration
5) Index regression: 2-8 standards are permitted to set. Through these standard points, an
If get the logarithm ( Y = LnY ) of absorbance value, index regression can be changed
6) Logarithmic regression: 4-8 standards are permitted to set. Throgh these standard points,
concentration values of all the standard preparations must be more than zero.
If get the logarithm of the concentration value ( X = LnX ), logarithmic regression can
7) Power regression: 2-8 standards are permitted to set. Through these standard points, a
and concentration values of all the standard preparations must be more than zero.
If get the logarithm of concentration value and absorbance value ( X = LnX
Y = LnY ), power regression can be changed to the form of linear regression:
Y = k X + b
8Percentage logarithmic regression
Percentage logarithmic regression permits to set 4-8 standards, and the concentration of
each standard preparation must be more than 1.
9Four-parameter regression
Four-parameter regression permits to set 4-8 standards. Input the concentration values
according to the corresponding reagent instructoin manual, use instrument to test the
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absorbance value of standard solutions, and get the regression curves by Gauss-Newton
Iteration Method.
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Controls can be set discretionarily, but its number of each test item should not exceed 12,
and take the mean value of all the control absorbances while calculating.
5Judgement of validity
Judgement of validity requires user to select correctly for each item from formula base.
Formula is the key point of the judgement of validity. Menu of the judgement of validity is as
show in Figure 5-6.
Note: User must input rational formula; otherwise unnecessary warning will
be produced in the test.
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6. Sample test
Sample test menu mainly includes the select of template typetest modecalculation
methodsettings of set plate parameterplate moving parameter and the microwell settings
on the Microplate. Microplate settings mainly include settings of sample type and the select of
blank mode.
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6.5.1. Sample
User click the hole position to be set as sample discretionarily, the sample number increase
from 1 automatically and is displayed at this hole position. If user wants to change sample
number, double-click the hole position:
Input sample number again in the window that emerged. If the sample number inputed has
been existed, system will display that the sample number has been existed, the change is
not valid. The range of sample number that is permitted for Microplate Reader is 1-999.
6.5.2. Blank
Blank hole is adopted for zero settings of absorbance in item testwhich means absorbance
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of other holes should reduce the absorbance value of blank hole. According to the
requirement of different item testuser may select whether set blank or not.
The patterns of blank include no blank; single point blank, multiple blank, single column blank,
column average blank, single row blank, row average blank.
If users select single row blank or all rows blank, only need to click the row mark at the left of
the microplate.
If users select single column blank or all columns blank, only need to click the column mark
on the upper of microplate.
Saving and application of old blanks: The test results of blank holes will be automatically
saved. If no blank is reset in the following tests of the same item, the system will
automatically use the old blank to calculate.
Be effective only in the item that calculation method is Cut-Off. According to the Cut-Off
formulauser must set negative control except for the condition that negative control
coefficient is 0. Each item is permited to set 12 negative controls at the most.
When item test is running, absorbance of control hole and the calculation results of
corresponding Cut-Off value will be saved automatically. In the following test of the same
item, if control is not set again, system will adopt the old Cut-Off value to calibrate.
6.5.6. Standard
It become effective when Item settings need calibration with standard.If this item has tested
old standard, user can select not to set standard (use old standard to calibrate)or to set all
standards again. The condition that only a part of standards are set is forbidden. User click
standard hole position successively, and system will successively mark them as SD1SD2
The settings of all standards are finished if user click again, system will display The
number of standard is enough!.If user begin to test or add new item before the setup is
finished, system will displays that The item of () hasn't enough standard!
6.5.8. Clear
If user need to revise the hole position that has been set, after clicking clear switch, user can
clear all set hole position of current item, and then setup again according to your
requirement.
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6.7. Test
Clickmeasurebuttonsystem displays the dialogue frame of being measuring.
Clicking stop key button can cancel current test.
6.11. Print
Clickprintbuttonthe whole board results of current test (include hole position, test item,
sample number, absorbance, quantitative result, qualitative result) can be printed according
to the item and the form of microplate.
6.12. Save
Clicksavethen the test result of this board can be saved in database for the later
inquirement.
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7. Result review
Result review includes test resultquality control informationcalibration curve and system
log.
This window includes three parts, Report by plate, Report by item and Report by patient.
For each part, results can be inquired or printed according to the current day, in one week
and user-defined time period.
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Select item in the Item name list frame, select batch number in the batch number list frame,
and select the time period to be inquired. Clicksearchbutton.
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8. System settings
Error warning
Mainly record the warning condition of the instrument, as show in Figure 8-2.
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9. Data maintenance
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Microplate Reader is a precise clinical analytic instrment. In order to keep the instrument in a
good state, routine maintenance must be done. The maintenance of Microplate Reader is
very simple, but it must be done carefully.
Warning: Please dont let any solvent, lip and erosive material touch the
instrument.
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Printer manifests as
Change ink box, and clean the
color fading and the
printing head. (Refer to the printer
whole print quality
users manual for details)
descends.
MRC.Ver.01-12.09
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