REP13/MAS Appendix II 1

Appendix II

ENDORSEMENT OF METHODS OF ANALYSIS PROVISIONS IN CODEX STANDARDS

A. Committee on Fish and Fishery Products
B. FAO/WHO Coordinating Committee for Asia
C. Committee on Processed Fruits and Vegetables
D. FAO/WHO Coordinating Committee for the Near East
E. Committee on Fats and Oils
F. Committee on Nutrition and Foods for Special Dietary Uses
G. Committee on Milk and Milk Products
H. Committee on Sugars
I. Committee on Contaminants in Foods
REP13/MAS Appendix II 2

A. COMMITTEE ON FISH AND FISHERY PRODUCTS

Standard for Smoked Fish, Smoke-Flavoured Fish and Smoke-Dried Fish
COMMODITY PROVISION METHOD PRINCIPLE Notes and Type
Smoked Fish, Smoke- Water phase salt AOAC 952.08 Type I
Flavoured fish and
Smoke-dried fish AOAC 937.09

Described in standard1 Calculation

Smoked Fish, Smoke- Water activity NMKL 168, 2001 Electrometry Type III
Flavoured fish and
Smoke-dried fish
Smoked Fish, Smoke- Water activity Electrometry Type III
Flavoured fish and ISO 21807:2004
Smoke-dried fish

Method Performance Criteria for histamine in smoked fish, smoke-flavoured fish and smoke-dried fish
Provision ML Minimum LOD (mg/100 LOQ (mg/100 RSDR(%) Recovery Suggested methods that meet the Principle
(mg/100 applicable g) g) criteria
g) range (mg/100
g)
Histamine 10 8 – 12 1 2 16.0 90 – 107 AOAC 977.13 | NMKL 91, 1987 Fluorometric
(average)
NMKL 196, 2013 HPLC
histamine 20 16 – 24 2 4 14.4 90 – 107 AOAC 977.13 | NMKL 91, 1987 Fluorometric
(each
NMKL 196, 2013 HPLC
unit)

Standard for Live Abalone and for Raw Fresh Chilled or Frozen Abalone for Direct Consumption or for Further Processing
COMMODITY PROVISION METHOD PRINCIPLE Notes and Type

1 % salt x 100 / (%water + %salt)

REP13/MAS Appendix II 3

frozen abalone Net weight AOAC 963.18 Gravimetry Type I

(covered by glaze)

B. FAO/WHO COORDINATING COMMITTEE FOR ASIA

Regional Standard for Tempe
COMMODITY PROVISION METHOD PRINCIPLE Notes and Type
Tempe Moisture content AOAC 925.09 | AACCI 44-40.01 Gravimetry (vacuum oven) type I
Tempe Protein content Titrimetry, Kjeldahl type I
NMKL 6, 2004 or AOAC 988.05 or AACCI 46-16.01 digestion
(Nitrogen factor6.25)
Tempe Lipid Content AOAC 983.23 Gravimetry type I
Tempe Crude fibre ISO 5498:1981 or AOAC 962.09 or AACCI 32-10.01 Gravimetry type I
Regional Standard for Non-Fermented Soybean Products
COMMODITY PROVISION METHOD PRINCIPLE Notes and Type
Non-fermented Moisture content AOAC 925.09 | AACCI 44-40.01 Gravimetry (vacuum oven) type I
soybean products
Non-fermented Protein content Titrimetry, Kjeldahl type I
soybean products NMKL 6, 2004 or AACCI 46-16.01 or AOAC 988.05 or AOCS digestion
Bc 4-91 or AOCS Ba 4d-90(Nitrogen factor6.25)

C. COMMITTEE ON PROCESSED FRUITS AND VEGETABLES

1. Methods of Analysis
Standard for Canned Apple Sauce
COMMODITY PROVISION METHOD PRINCIPLE Notes and Type
Canned Apple Fill of containers CAC/RM 46-1972* (for glass containers) Weighing Type I
Sauce (Codex general method for processed fruits and vegetables)
and
ISO 90-1.1:1999 (for metal containers)
(Codex general method for processed fruits and vegetables)
Canned Apple Soluble solids AOAC 932.12 Refractometry Type I
Sauce ISO 2173:2003
(Codex general method for processed fruits and vegetables)
REP13/MAS Appendix II 4

Standard for Table Olives

COMMODITY PROVISION METHOD PRINCIPLE Notes and Type
Table olives Drained weight AOAC 968.30 Sieving Type I
(Codex general method for processed fruits and vegetables) Gravimetry
Table olives Fill of containers CAC/RM 46-1972* (for glass containers) Weighing Type I
(Codex general method for processed fruits and vegetables)
and
ISO 90-1.1:1999 (for metal containers)
(Codex general method for processed fruits and vegetables)
Table olives pH of brine NMKL 179:2005 Potentiometry type II
(Codex general method for processed fruits and vegetables)
Table olives AOAC 981.12 Type III
(Codex general method for processed fruits and vegetables)
Table olives ISO 1842:1991 Type IV
Table olives Salt in brine AOAC 971.27 | NMKL 178, 2004 Potentiometry Type II
(Codex general method)
Table olives ISO 3634:1979 Type III
“chloride expressed as sodium chloride”
(Codex general method for processed fruits and vegetables)
Table olives Lead AOAC 999.11 | NMKL 139, 1991 (Codex general method) AAS (Flame absorption) Type II
Table olives Tin AOAC 980.19 (Codex general method) AAS Type II
* DETERMINATION OF WATER CAPACITY OF CONTAINERS (CAC/RM 46-1972)
1. SCOPE
This method applies to glass containers.
2. DEFINITION
The water capacity of a container is the volume of distilled water at 20°C which the sealed container will hold when completely filled.
3. PROCEDURE
3.1 Select a container which is undamaged in all respects.
3.2 Wash, dry and weigh the empty container.
3.3 Fill the container with distilled water at 20°C to the level of the top thereof, and weigh the container thus filled.
4. CALCULATION AND EXPRESSION OF RESULTS
Subtract the weight found in 3.2 from the weight found in 3.3. The difference shall be considered to be the weight of water required to fill the container. Results are expressed as mL of water.

Aqueous coconut products

REP13/MAS Appendix II 5

Products Provisions Method Principle Type

Aqueous Total Fats ISO 1211:1999 Gravimetry (Röse-Gottlieb) I
coconut IDF 1D:1996
products ISO 1211|IDF 1:2010
Aqueous Totals Solids ISO 6731:1989 Gravimetry I
coconut IDF 21B:1987
products ISO 6731|IDF 21:2010
Aqueous Non-fat solids ISO 1211:1999 Calculation: I
coconut IDF 1D:1996 Gravimetry (Röse-Gottlieb)
products ISO 1211|IDF 1:2010
And
ISO 6731:1989 Gravimetry
IDF 21B:1987
ISO 6731|IDF 21:2010
Aqueous Moisture ISO 6731:1989 Gravimetry I
coconut IDF 21B:1987
products ISO 6731|IDF 21:2010

2. Sampling
Commodity Sampling Plan Status
Table Olives Described in the Standards Endorsed

D. FAO/WHO COORDINATING COMMITTEE FOR THE NEAR EAST

Regional Standard for Date Paste
COMMODITY PROVISION METHOD PRINCIPLE Notes and Type
Date Paste Moisture AOAC 934.06 Gravimetry Type I
Date Paste Mineral matter ISO 762:2003 Gravimetry Type I
content
Date Paste Ash AOAC 940.26 Gravimetry Type I
Date Paste Acid Soluble Ash AOAC 900.02D Gravimetry Type I
Calculation
REP13/MAS Appendix II 6

Regional Standard for Halwa Tehenia

COMMODITY PROVISION METHOD PRINCIPLE Notes and Type
Halwa Tehenia Sugars ISI 28-1e2 Polarimetry Type IV
Halwa Tehenia Acidity AOAC 924.53, AOAC 942.15 Titrimetry Type IV

E. COMMITTEE ON FATS AND OILS

Standard on Olive Oil and Olive Pomace Oil
COMMODITY PROVISION METHOD PRINCIPLE Notes and Type
Olive Oils and Erythrodiol + COI/T.20/doc.No 30-2011 Gas chromatography Type II
Olive Pomace uvaol
Oils

F. COMMITTEE ON NUTRITION AND FOODS FOR SPECIAL DIETARY USES

Products Provisions Method Principle Note and Type
Special foods Loss on drying (milk AOAC 925.23 Gravimetry Type I
based) IDF Standard 21B:1987 ISO 6731:1989
ISO 6731|IDF 21:2010
Special foods Sodium and ISO 8070:1987 (confirmed 1992) Flame emission atomic Type II
Potassium IDF Standard 119A:1987 absorption spectrometry
ISO 8070|IDF 119:2007
Infant formula Moisture/Total AOAC 990.20 Gravimetry Type I
Solids IDF 21B:1987 or
ISO 6731:1989
ISO 6731|IDF 21:2010

G. COMMITTEE ON MILK AND MILK PRODUCTS

Products Provisions Method Principle Type
Edible Casein Products Casein in protein ISO 17997-1|IDF 29-1:2004 Titrimetry, Kjeldahl I

2
http://www.starch.dk/isi/methods/28luff.htm
REP13/MAS Appendix II 7

Milk products (products not Milk fat IDF 124-3|ISO 8262-3:2005 Gravimetry (Weibull- I
completely soluble in ammonia) Berntrop)
see Appendix III – Part D of the ISO 8262-3|IDF 124-3:2005
Alinorm 08/31/23 - CCMAS
2008
Blend of evaporated skimmed Total fat ISO 1737| IDF 13:2008 Gravimetry (Röse-Gottlieb) IV I
milk and
vegetable fat
See Appendix II Alinorm
10/33/23 CCMAS 2010
Blend of evaporated skimmed Milk solids-not- ISO 6731|IDF 21:2010 Calculation from total I
milk and fat*1(MSNF) and solids content and fat
vegetable fat ISO 1737| IDF 13:2008 content
See Appendix II Alinorm I
10/33/23 CCMAS 2010 Gravimetry (Röse-Gottlieb)
Reduced fat blend of evaporated Total fat ISO 1737| IDF 13:2008 Gravimetry (Röse-Gottlieb) IV I
skimmed milk and vegetable fat
See Part B - Appendix II
Alinorm 10/33/23 CCMAS 2010
Blend of sweetened condensed Milk solids-notfat ISO 6734|IDF 15:2010 Calculation from total IV
skimmed (MSNF)2 solids content, fat content
milk and vegetable fat and sugar content.
See Appendix II REP12MA –
CCMAS 2012
Reduced fat blend of sweetened MSNF2 ISO 6734|IDF 15:2010 Calculation from total IV
condensed solids content, fat content
skimmed milk and vegetable fat and sugar content
See Appendix II REP12MA –
CCMAS 2012.
Reduced fat blend of skimmed Water2 ISO 5537|IDF 26:2004 Gravimetry, drying at 87 °C I
milk powder and vegetable fat
in powdered form
Bookmark error
Butter Milk solids-not-fat1 ISO 3727-2|IDF 80-2:2001 Gravimetry I

Butter Milkfat IDF 194|ISO 17189:2003 Gravimetry I

See Appendix III – Part D of the ISO 17189|IDF 194:2003 Direct determination of fat
Alinorm 08/31/23 - CCMAS using solvent extraction
2008
Butter Salt IDF 179|ISO 15648:2004 Potentiometry III II
See Appendix III – Part D of the ISO 15648|IDF 179:2004 (determination of chloride,
REP13/MAS Appendix II 8

Alinorm 08/31/23 - CCMAS expressed as sodium

2008 chloride)
Butter Salt IDF 12|ISO 1738:2004 Titrimetry (Mohr: II III
See Appendix III – Part D of the ISO 1738|IDF 12:2004 / determination of
Alinorm 08/31/23 - CCMAS AOAC 960.29 chloride, expressed as
2008 sodium chloride)
Butter Water2 IDF 80-1|ISO 3727-1:2001 Gravimetry I
See Appendix III – Part D of the ISO 3727-1|IDF 80-1:2001
Alinorm 08/31/23 - CCMAS
2008
Cheese (and cheese rind) Natamycin ISO 9233-1|IDF 140-1:2007 Molecular absorption III
spectrophotometry
See Part B - Appendix II ISO 9233-2|IDF 140-2:2007 HPLC II
Alinorm 10/33/23 CCMAS 2010
Cheeses, individual Milk fat in dry ISO 1735|IDF 5:2004 Gravimetry (Schmid- I
See Appendix III – Part D of the matter Bondzynski-
Alinorm 08/31/23 - CCMAS Ratzlaff)
2008

Cheeses in brine Milk fat in dry IDF 5|ISO 1735:2004 Gravimetry (Schmid- I
See Appendix III – Part D of the matter (FDM) ISO 1735|IDF 5:2004 Bondzynski-Ratzlaff)
Alinorm 08/31/23 - CCMAS
2008

Creams lowered in milk fat Milk fat ISO 2450|IDF 16:2008 / AOAC 995.19 Gravimetry (Röse- I
content Gottlieb)
See Part B - Appendix II
Alinorm 10/33/23 CCMAS 2010
Creams, whipped creams and MSNF1 ISO 3727-2|IDF 80-2:2001 AOAC 920.116 Gravimetry I
fermented creams
Edible casein products Copper IDF 76| ISO 5738:2004 Colorimetry III
See Appendix III – Part D of the ISO 5738|IDF 76:2004 (diethyldiethiocarbamate)
Alinorm 08/31/23 - CCMAS
2008
Edible casein products Lactose IDF 106|ISO 5548:2004 Photometry (phenol and IV
See Appendix III – Part D of the ISO 5548|IDF 106:2004 H2SO4)
Alinorm 08/31/23 - CCMAS
2008

Edible casein products Lead IDF/RM 133|ISO/TS 6733:2006 Spectrophotometry (1,5- IV

REP13/MAS Appendix II 9

See Appendix III – Part D of the ISO/TS 6733|IDF/RM 133:2006 diphenylthiocarbazone)

Alinorm 08/31/23 - CCMAS
2008
Edible casein products Water2 ISO 5550|IDF 78:2006 Gravimetry (drying at I
Bookmark error 102 °C)
Edible casein products Moisture ISO 5550|IDF 78:2006 Gravimetry (drying at I
See Appendix II – Part B of the 102 °C)
Alinorm 10/33/23 - CCMAS
2010
Fermented milks Lactic acid (total IDF 150:1991/ISO 11869:1997 ISO/TS Potentiometry, IV I
See Appendix II REP12MA – acidity expressed as 11869|IDF/RM 150: 2012 titration to pH 8.30
CCMAS 2012 lactic acid) Total Spectrophotometry
acidity expressed
as lactic acid
Milk powders and cream Water2 ISO 5537|IDF 26:20043 Gravimetry (drying at I
powders 87°C)
Bookmark error

Milk fat products Milk fat IDF 24:1964 Gravimetry (calculation IV

See Appendix II REP12MA – from solids-not-fat content
CCMAS 2012 and water content)
Processed cheese products Citric acid ISO/TS 2963|IDF/RM 34:2006 Enzymatic method IV
See Part B - Appendix II
Alinorm 10/33/23 - CCMAS
2010
Processed cheese products Citric acid AOAC 976.15 Photometry III
See Part B - Appendix II
Alinorm 10/33/23 - CCMAS
2010
Processed cheese products Milkfat ISO 1735|IDF 5:2004 Gravimetry (Schmid- I
See Part B - Appendix II Bondzynski-Raztlaff)
Alinorm 10/33/23 - CCMAS
2010
Processed cheese products Phosphate, added IDSF 51B:1991 Calculation from IV
See Part B - Appendix II (expressed as phosphorous and nitrogen
Alinorm 10/33/23 - CCMAS phosphorus) content
2010

3
The method has only been validated for milk powders, not for cream powders
REP13/MAS Appendix II 10

Processed cheese products Phosphorus IDF 33C:1987/ISO 2962:1984 Spectrophotometry II

See Part B - Appendix II (molybdate-ascorbic acid)
Alinorm 10/33/23 - CCMAS
2010
Processed cheese products Salt ISO 5943|IDF 88:2004 Potentionmetry II
See Part B - Appendix II (determination of chloride,
Alinorm 10/33/23 - CCMAS expressed as sodium
2010 chloride)
Sweetened condensed milk Milk fat ISO 1737:1999 ISO 1737| IDF 13:2008 Gravimetry (Röse-Gottlieb) I
See Part B - Appendix II
Alinorm 10/33/23 - CCMAS
2010
Whey Cheese Dry matter (for IDF 58 | ISO 2920:2004 Gravimetry, drying at 88 °C I
See Appendix III – Part D of the denomination)
Alinorm 08/31/23 - CCMAS
2008
Whey cheeses by concentration Dry matter (total IDF 58 | ISO 2920:2004 Gravimetry, drying at 88 °C I
See Appendix III – Part D of the solids)
Alinorm 08/31/23 - CCMAS
2008
Whey cheeses by coagulation Dry matter (total IDF 4:2004 Gravimetry, Drying at IV
See Appendix III – Part D of the solids) ISO 5534:2004 102°C
Alinorm 08/31/23 - CCMAS
2008

Whey cheese Fat on the dry basis IDF 59 A:1986 / ISO 1854:1999 Calculation from fat content I
See Appendix III – Part D of the and and dry matter content
Alinorm 08/31/23 - CCMAS IDF 58:2004 |
2008 ISO 2920:2004
REP13/MAS Appendix II 11

Whey cheese Milkfat (in dry IDF standard 59A:1986 / ISO 1854:1999 Gravimetry (Röse-Gottlieb) I
See Appendix III – Part D of the matter)
Alinorm 08/31/23 - CCMAS
2008
Whey cheeses including whey Total fat IDF 59A:1986 / ISO 1854:1999 Gravimetry (Röse Gottlieb) I
cheeses by concentration
See Appendix III – Part D of the
Alinorm 08/31/23 - CCMAS
2008
Whey cheeses by coagulation Total fat IDF 5:2004 | Gravimetry (Schmid- I
See Appendix III – Part D of the ISO 1735:2004 Bondzynski-Ratzlaff
Alinorm 08/31/23 - CCMAS
2008
Creamed whey cheese Fat on the dry basis IDF 59 A: 1986 / ISO 1854: 1999 Calculation from fat content I
See Appendix III – Part D of the and and dry matter content
Alinorm 08/31/23 - CCMAS IDF 58:2004 |
2008 ISO 2920:2004
Skimmed whey cheese Fat on the dry basis IDF 59 A:1986 / ISO 1854:1999 Calculation from fat content I
See Appendix III – Part D of the and and dry matter content
Alinorm 08/31/23 - CCMAS IDF 58:2004 |
2008 ISO 2920:2004
Whey cheeses by coagulation Milk fat ISO 1735|IDF 5:2004 Gravimetry (Schmid-Bondzynski-Ratzlaff) I
See Appendix III – Part D of the
Alinorm 08/31/23 - CCMAS
2008
Whey cheeses by coagulation Milk fat in dry Calculation from fat content and dry matter I
See Appendix III – Part D of the matter content
Alinorm 08/31/23 - CCMAS ISO 1735|IDF 5:2004 Gravimetry (Schmid-Bondzynski-Ratzlaff)
2008 and Gravimetry, drying at 102 °C
ISO 5534|IDF 4:2004
Whey cheeses by Milk fat ISO 1854|IDF 59:2008 Gravimetry (Röse Gottlieb) I
concentration
See Appendix III – Part D of the
Alinorm 08/31/23 - CCMAS
2008
Whey cheeses by Milk fat in dry Calculation from fat content and dry matter I
concentration matter content
See Appendix III – Part D of the ISO 1854|IDF 59:2008 and Gravimetry (Röse Gottlieb)
Alinorm 08/31/23 - CCMAS ISO 2920|IDF 58:2004 Gravimetry, drying at 88 °C
2008
Yoghurt products Lactobacillus IDF 117 | ISO 7889:2003 Colony count at 37°C
REP13/MAS Appendix II 12

See Part B - Appendix II bulgaricus &

Alinorm 10/33/23 - CCMAS Streptococcus
2010 thermophilus
Yoghurt products Lactobacillus IDF 146 | ISO 9232:2003 Test for identification
See Part B - Appendix II bulgaricus &
Alinorm 10/33/23 - CCMAS Streptococcus
2010 thermophilus
Yoghurt products Solids, Total IDF 151 | ISO 13580:2005 Gravimetry (drying at 102°C) I
See Part B - Appendix II
Alinorm 10/33/23 - CCMAS
2010

Yoghurt Streptococcus ISO 7889/IDF 117:2003 Colony count at 37°C I

thermophilus &
See Part B - Appendix II
Lactobacillus
Alinorm 10/33/23 - CCMAS
delbrueckii subsp.
2010
bulgaricus
>= 107 cfu/g

Yoghurt Streptococcus ISO 9232/IDF 146:2003 Test for identification: morphological, cultural I
thermophilus & and biochemical characteristics
See Part B - Appendix II
Lactobacillus
Alinorm 10/33/23 - CCMAS
delbrueckii subsp.
2010
bulgaricus
>= 107 cfu/g

H. Committee on Sugars
Products Provisions Method Principle Note and Type
Honey diastase activity IHC Method for Determination of Diastase activity Type IV
with Phadebas, 2009 except that the incubation time
should be increased from 15 to 30 minutes.

I. Committee on Contaminants in Foods

Commodity Sampling Plan Status
Dried Figs Described in the Standard Endorsed