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Journal of Sport and Health Science 7 (2018) 139
148

www.jshs.org.cn

Original article
Fitness and health benefits of team handball training for young untrained
women—A cross-disciplinary RCT on physiological adaptations and
motivational aspects
TagedPTherese Hornstrup a, Johan M. Wikman a, Bjørn Fristrup a, Susana Povoas b, Eva W. Helge a,
Signe H. Nielsen a, Jørn W. Helge c, Jesper L. Andersen d, Lars Nybo a, Peter Krustrup e,f,*
TagedP Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen N 2200, Denmark
a
b
Research Center in Sports Sciences, Health Sciences and Human Development, CIDESD, University Institute of Maia, ISMAI, Maia 4475-690, Portugal
c
Center of Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Copenhagen N 2200, Denmark
d
Institute of Sports Medicine Copenhagen, Bispebjerg Hospital, Copenhagen NV 2400, Denmark
e
Department of Sports Science and Clinical Biomechanics, SDU Sport and Health Sciences Cluster (SHSC), University of Southern Denmark,
Odense 3450, Denmark
f
Sport and Health Sciences, University of Exeter, Exeter, EX1 2LU, UK
Received 6 June 2017; revised 7 July 2017; accepted 19 August 2017
Available online 19 September 2017

TagedPAbstract
Purpose: The present study evaluated the effects of regular participation in small-sided team handball training on body composition, osteogenic
response, physical performance, and cardiovascular risk factors, as well as well-being and motivation, in young untrained women.
Methods: Twenty-eight untrained 20- to 30-year-old women were randomized to a handball training group (HG; n = 14, height 170 § 5 cm, weight
73 § 11 kg, VO2peak 37.7 § 4.1 mL/min/kg) that trained 1.7 § 0.3 times per week over 12 weeks (70 min 4 v 4 handball sessions) or an inactive con-
trol group (CG; n = 14, 169 § 5 cm, 71 § 12 kg, 38.1 § 3.7 mL/min/kg). Physiological and psychological and motivational training adaptations were
assessed pre- and post-intervention by dual-energy X-ray Absorptiometry (DXA) scans, blood sampling, physical tests, and questionnaires.
Results: The average heart rate (HR) over all training sessions was equal to 85% § 6% HRmax. Between-group intervention effects were observed
in favor of HG for muscle mass (2.1%, p = 0.024), proximal femur bone mineral density (0.8%, p = 0.041), Yo-Yo IE1 intermittent endurance test
level 1 (IE1) performance (35%, p < 0.001), and incremental treadmill test performance (11.5%, p = 0.003), but not total fat mass (p = 0.176),
mean arterial blood pressure (p = 0.328), resting HR (p = 0.219), or blood lipids (p = 0.298
0.854). In CG, no changes were observed in any of
the measured physiological variables after the training period. Compared to CG, HG had an increase in intrinsic motivation (p < 0.001) and in
the well-being subscale “energy” (p = 0.010).
Conclusion: Participation in regular recreational team handball training organized as small-sided games has marked beneficial effects on physical
performance, musculoskeletal fitness, well-being, and motivation in untrained young women.
Ó 2018 Published by Elsevier B.V. on behalf of Shanghai University of Sport. This is an open access article under the CC BY-NC-ND license.
(http://creativecommons.org/licenses/by-nc-nd/4.0/).
TagedPKeywords: Bone mineral density (BMD); Intensity; Intermittent; Motivation; Muscle mass; Physical performance; Recreational handball; Well-being

1. Introduction TagedPassociated with a sedentary life.2 It has been shown that regu-
lar participation in physical activities can markedly benefit
TagedPThe prevalence of lifestyle diseases associated with low
individual health and that supervised training interventions
cardiovascular and metabolic fitness is reaching pandemic pro-
can be used for both the prevention and treatment of lifestyle
portions.1 Thus, the expected increases in people with type 2
diseases.3,4 However, the long-term adherence rates to exer-
diabetes (T2DM) signifies the major societal challenge
cise on prescription programs are poor.5
TagedPIn Denmark, the sporting associations Danmarks Idrætsfor-
Peer review under responsibility of Shanghai University of Sport. bund and Danmarks Gymnastik & Idrætsforeninger, supported
*
Corresponding author.
E-mail address: pkrustrup@health.sdu.dk (P. Krustrup).
by the Danish government, have a vision that, by 2025, 50%

https://doi.org/10.1016/j.jshs.2017.09.007
2095-2546/Ó 2018 Published by Elsevier B.V. on behalf of Shanghai University of Sport. This is an open access article under the CC BY-NC-ND license.
(http://creativecommons.org/licenses/by-nc-nd/4.0/)
140 T. Hornstrup et al.

TagedPof the Danish population should be members of a local sport- 2. Methods

ing association and 75% should be physically active.6 The
2.1. Subjects
sporting associations find it difficult keeping young members
interested in their teenage years and reaching out to sedentary TagedPForty-five untrained healthy 20- to 30-year-old women
people with no or little experience of sport where competition indicated an interest in participating in the study following an
is not a major element.6 Furthermore, it has been shown that initial meeting (Fig. 1). To be eligible for participation, the
the dropout rate with traditional “exercise on prescription pro- subjects were not allowed to have any known familiar dis-
grams” is high both in Denmark and worldwide,5,7 and new eases, to be using daily medication, or to have smoked in the
strategies for the prevention of lifestyle diseases should be past 2 years. Additionally, the subjects were obliged to have
aimed at identifying activities that can motivate participants to a regular menstrual cycle length (25 to 32 days) with or with-
remain active and hence provide an effective, long-lasting life- out use of contraceptives. Furthermore, the subjects were not
style change with prolonged physiological perspectives. Previ- allowed to have been involved in any regular physical train-
ous observations from studies conducted on team sport, ing for at least 2 years before recruitment. To ensure the sub-
performed as small-sided games, have shown marked benefi- jects were untrained, they were required to have a peak
cial health effects and development of social relationships in oxygen uptake (VO2peak) of 25
45 mL/min/kg. Three sub-
untrained sedentary men and women as well as people with jects did not meet the inclusion criterion for VO2peak and
hypertension, T2DM, and prostate cancer.8
19 However, differ- were excluded after the medical screening. Another 2 sub-
ent team sports may attract and motivate different populations jects dropped out during baseline testing for personal reasons
and increase individual incentive for sustained participation and illness, respectively. Forty subjects completed baseline
and therefore induce long-lasting lifestyle changes. We believe testing. After baseline testing, the subjects were stratified for
that handball is a team sport that may have great potential to VO2peak and body fat percentage and randomly assigned to
reach out to sedentary populations in Denmark and other Euro- either a handball training group (HG) (n = 20) or an inactive
pean countries because of its popularity in terms of television control group (CG) (n = 20). Two thirds of the subjects in HG
time, fan base, and appeal. However, it remains unknown had some experience with handball, except from playing in
whether small-sided handball training provides a physiological physical education classes in schools, but none had played
training stimulus that is as potent for fitness and health effects club-based handball for a number of years. Four subjects in
as small-sided football, while the motivational and psychologi- HG and 4 subjects in CG left the study for personal reasons.
cal aspects of participation in an intervention involving small- Another 2 in HG dropped out due to concussion outside the
sided handball training also remain unevaluated. study and an ankle injury during a training session, respec-
TagedPA recent study by P ovoas et al.17 concluded that handball tively. The last 2 in CG dropped out due to pregnancy and ill-
(7 v 7) in 33- to 55-year-old untrained men is an intermittent ness, respectively. For the participants who completed the
high-intensity exercise mode with physical and physiological study (FG, n = 14; CG, n = 14), there were no group differen-
demands in the range of those found to have a positive effect ces in baseline values for age or any of the physiological
on aerobic, anaerobic, and musculoskeletal fitness in adult parameters (Table 1). The subjects were informed of the risks
individuals. Similarly, it seems that team sports, like other
physical activity, can increase well-being in participants,16
and it can be expected that recreational handball will produce
similar effects in sedentary individuals. Also, little is known
about the effects of recreational handball on motivation in sed-
entary individuals. However, it seems that team sports in gen-
eral can be motivating for participants that are sedentary or
unfamiliar with sports club participation.15,16,19 From the per-
spective of self-determination theory,20 team sports seem to be
intrinsically motivating, meaning that individuals take part in
the activity for the sake of the activity itself.21 Research sug-
gests that intrinsic motivation is a strong predictor of participa-
tion and adherence.15,22
TagedPWe hypothesized that recreational handball could provide
broad-spectrum fitness and health benefits analogously to rec-
reational football, with combined effects on cardiovascular,
metabolic and musculoskeletal fitness. The present study was
therefore conducted with the aim of providing a novel and
cross-disciplinary evaluation of cardiovascular, metabolic, and
musculoskeletal adaptations, as well as well-being and motiva-
tional aspects of health, in young untrained healthy 20- to
30-year-old women following a 12-week period of small-sided Fig. 1. Flow chart showing the process for recruiting untrained healthy 20- to
4 v 4 handball training. 30-year-old female subjects.
Fitness and health benefits of women’s team handball 141

Table 1
Age, body composition, bone mineral density and bone markers, exercise endurance capacity, cholesterol, systolic and diastolic blood pressure, HbA1c, well-being
and motivation subscale values in young untrained women before (0 week) and after 12 weeks of small-sided handball training (HG) or continuation of an inactive
lifestyle (CG) (mean § SD).

HG (n = 14) CG (n = 14) ANOVA Group £ time

0 week 12 weeks 0 week 12 weeks Time Group
Age (year) 23.9 § 2.4 24.2 § 2.4 24.1 § 3.2 24.1 § 3.2



Weight (kg) 73.0 § 11.0 73.3 § 10.1 70.8 § 11.8 70.8 § 11.9 0.376 0.575 0.393
Height (cm) 169.9 § 4.9
169.1 § 5.1




Total muscle mass (kg) 43.5 § 4.3 44.4 § 4.5***,# 42.8 § 4.9 42.9 § 5.1 0.007 0.535 0.024
Total fat percentage (%) 36.4 § 4.9 35.4 § 4.8* 35.4 § 5.5 35.3 § 5.5 0.025 0.759 0.072
Total fat mass (kg) 27.0 § 7.3 26.4 § 7.0y 25.5 § 7.7 25.4 § 7.7 0.078 0.658 0.176
Proximal femur BMD (g/cm2) 1.084 § 0.094 1.092 § 0.087*,# 1.047 § 0.129 1.045 § 0.133 0.256 0.330 0.041
CTX-I (mg/L) 0.38 § 0.14 0.51 § 0.29* 0.40 § 0.26 0.41 § 0.23 0.037 0.630 0.063
P1NP (mg/L) 52.7 § 25.7 70.0 § 28.3* 55.4 § 34.6 56.7 § 36.5 0.046 0.648 0.083
Osteocalcin (mg/L) 20.6 § 6.8 32.1 § 8.8***,# 23.1 § 9.5 24.3 § 9.7 <0.001 0.423 <0.001
VO2peak (mL/min/kg) 37.7 § 4.1 38.1 § 3.7 38.1 § 3.7 37.6 § 3.7 0.129 0.234 0.124
Yo-Yo IE1 performance (m) 1073 § 318 1452 § 397***,# 1151 § 509 1169 § 574 <0.001 0.567 <0.001
TTE (s) 393 § 70 438 § 64**,# 398 § 101 387 § 106 0.059 0.464 0.003
HR at 8 km/h (bpm) 169 § 12 158 § 8* 167 § 11 163 § 15 <0.001 0.568 0.056
TC (mmol/L) 4.5 § 0.6 4.5 § 0.5 4.4 § 1.0 4.2 § 1.1 0.606 0.323 0.854
HDL-C (mmol/L) 1.5 § 0.3 1.6 § 0.4 1.5 § 0.4 1.5 § 0.3 0.463 0.647 0.298
LDL-C (mmol/L) 2.6 § 0.6 2.4 § 0.5 2.6 § 0.9 2.4 § 0.9 0.048 0.866 0.682
TG (mmol/L) 1.1 § 0.5 1.2 § 0.5 0.8 § 0.4 0.9 § 0.4 0.261 0.086 0.618
Systolic blood pressure (mmHg) 103 § 8 102 § 7 103 § 12 102 § 12 0.926 0.961 0.311
Diastolic blood pressure (mmHg) 69 § 5 67 § 5 67 § 8 68 § 8 0.433 0.951 0.123
Mean arterial pressure (mmHg) 80 § 6 78 § 5 79 § 9 79 § 9 0.328 0.989 0.328
Resting HR (bpm) 59 § 9 57 § 8 61 § 8 62 § 8 0.465 0.280 0.219
HbA1c (mmol/L) 5.2 § 0.4 5.2 § 0.4 5.2 § 0.3 5.1 § 0.4 0.291 0.844 0.378
Anxiety 1.42 § 0.32 1.40 § 0.30 1.53 § 0.62 1.74 § 0.53 0.210 0.202 0.112
Energy 2.67 § 0.44 3.06 § 0.64*,# 2.79 § 0.71 2.65 § 0.69 0.219 0.533 0.010
Positive well-being 3.12 § 0.42 3.14 § 0.46 3.11 § 0.65 2.93 § 0.80 0.362 0.633 0.225
Intrinsic motivation to accomplish 4.23 § 1.26 5.12 § 0.99***,# 4.75 § 1.17 3.60 § 1.18** 0.603 0.217 <0.001
Intrinsic motivation to know 3.77 § 1.20 4.35 § 0.97y 3.71 § 1.07 2.85 § 1.33**,# 0.534 0.067 0.003
Intrinsic motivation to experience stimulation 5.08 § 0.64 5.42 § 0.99 4.75 § 1.18 4.65 § 1.15 0.539 0.130 0.258
Extrinsic motivation identified regulation 3.77 § 0.94 4.12 § 1.12 3.56 § 1.30 3.63 § 1.07 0.410 0.359 0.566
Extrinsic motivation introjected regulation 4.44 § 1.02 4.62 § 1.23 5.19 § 1.72 4.63 § 1.22 0.399 0.436 0.118
Extrinsic motivation external regulation 2.65 § 1.16 3.14 § 1.52 3.08 § 1.54 3.31 § 1.60 0.206 0.562 0.649
#
Time £ group interaction.
* p < 0.05, ** p < 0.01, *** p < 0.001, significantly different from 0 week within group. y p < 0.1, tended to increase or decrease, compared with 0 week within
group.
Abbreviations: BMD = bone mineral density; CTX-1 = carboxy-terminal type-1 collagen crosslinks; HbA1c = glycosylated haemoglobin; HDL-C = high-density
lipoprotein cholesterol; HR = heart rate; LDL-C = low-density lipoprotein cholesterol; P1NP = procollagen type-1 amino-terminal propeptide; TC = total choles-
terol; TG = triglyceride; TTE = time to exhaustion; VO2peak = peak oxygen uptake; Yo-Yo IE1 = Yo-Yo intermittent endurance test level 1.

TagedPand discomforts associated with the experimental procedures, TagedP1—submaximal and progressive maximal treadmill testing;
and all provided written consent. The study was carried out test Day 2—dual-energy X-ray Absorptiometry (DXA) scans
in accordance with the guidelines set out in the Declaration (iDXA; Lunar Corporation, Madison, WI, USA) (whole-body
of Helsinki and was approved by the National Committee on and proximal femur), blood pressure and resting heart rate
Health Research Ethics of Region Hovedstaden (Reference (HR) measurements, fasting blood samples, and well-being
No.: H-15008361). questionnaires; test Day 3—Yo-Yo intermittent endurance test
level 1 (Yo-Yo IE1); test Day 4—muscle strength and balance
tests. Data from test Day 4 are not covered in this article.
2.2. Training and testing procedures
TagedPThe participants in HG were trained for 12 weeks, while the
2.3. Training intervention
subjects in CG continued their normal everyday activities dur-
ing the same period. The subjects were tested on 4 separate TagedPIndoor handball training was performed for 70 min twice a
days before the study and after the 12-week intervention week for 12 weeks at the Department of Nutrition, Exercise
period. The study represented a comprehensive interventional and Sports, University of Copenhagen. The subjects in HG
protocol investigating musculoskeletal and cardiovascular chose from 3 weekly training sessions, and for each subject
adaptions as well as changes in physical performance and there was at least 40 h between training sessions. The handball
health status for the participants. The study included test Day training consisted of a 12 min warm-up followed by
142 T. Hornstrup et al.

TagedP4 £ 10 min periods of match-play (mainly 4 v 4, sometimes 2.7. Incremental treadmill testing (VO2peak) and intermittent
3 v 3) on a 14 m wide and 19 m long handball pitch inter- endurance performance
spersed with 3 min breaks. The average number of training
TagedPBefore and after the 12-week intervention period, pulmonary
sessions during the intervention period was 21 § 4, corre-
gas exchange (OxyconPro; Viasys Healthcare, Hoechberg, Ger-
sponding to 1.7 § 0.3 sessions per week. All, except 1 partici-
many) and HR (Polar Team System; Polar Electro Oy, Kemple,
pant, attended training >1.5 times per week on average.
Finland) were measured during a standardized incremental
treadmill test. The test protocol consisted of 5 min at each of 2
2.4. Habitual physical activity level steady-state speed levels (6 and 8 km/h) followed by 4 min rest
TagedPThe subjects were told not to change their normal diet dur- periods. Average VO2 and average HR values were determined
ing the 12-week intervention period. In addition, the subjects from 3 to 4 min of exercise at 6 and 8 km/h, respectively. The
were told not to engage in other regular sporting activities in incremental test to exhaustion was carried out after the break,
their spare time beyond the handball training for HG. The sub- starting at 8 km/h for 2 min and then increasing by 1 km/h
jects were instructed to report any changes. Furthermore, the every 1 min until total exhaustion. A leveling off in oxygen
amount of daily activity was measured using the International uptake with an increase in work rate at the end of the test was
Physical Activity Questionnaire. This questionnaire measures used to objectively confirm achievement of VO2peak. VO2 was
vigorous and moderate physical activity during work, in the determined as mean values over 30 s and VO2peak was defined
garden, in the home, during transport, and in leisure time. by the highest 30 s mean value. Time to exhaustion (TTE) was
Except for during leisure time, where there would be a change noted and maximal heart rate (HRmax) was determined as the
due to the handball training, the results showed that the sub- highest value measured during a 15 s period.
jects had no changes in activity patterns in relation to trans- TagedPA Yo-Yo IE1 test was performed to determine intermittent
port, gardening, and work during the 12-week intervention endurance capacity before and after the 12-week intervention
(results not shown). period. The test consisted of 2 £ 20 m shuttle runs performed
at increasing speeds interspersed with 5 s of active recovery
2.5. Measurements and test procedures during which the participants jogged around a cone placed
2.5 m behind the starting
finishing line. The speeds were con-
TagedPSubjects were given detailed instructions in preparation trolled by audio signals from a CD. The test was performed
for the pre- and post-testing. No exhausting physical activi- indoors on a wooden floor after a standardized 10 min low-
ties were allowed 2 days before testing and the subjects were intensity warm-up. The test was terminated when the subject
to refrain from alcohol and caffeine consumption 24 h and was no longer able to maintain the required speed. The total
12 h before testing, respectively. In addition, test Day 2, distance (m) covered represented the test result for each indi-
involving DXA scans, blood sampling, blood pressure meas- vidual. HRmax was determined as the highest value measured
urements, and peripheral endothelial function assessments during a 15 s period.
between 7 a.m. and 10 a.m., followed an overnight fast. Most
of the women’s hormone levels were constant during the
2.8. Blood pressure and resting HR
menstrual cycle due to their intake of contraceptives, but for
those who did not use contraceptives test Day 2 was sched- TagedPSystolic and diastolic blood pressure as well as resting HR
uled between Days 5 and 12 in the mid-follicular phase were measured 6 times with the subject in the supine position
when progesterone is low and before ovulation. This was using an automatic upper-arm blood pressure monitor (M7;
done to reduce the impact of changes in hormone levels on OMRON, Vernon Hills, IL, USA) and an average value was
test results during pre- and post-testing. In a few cases, it calculated. The measurements were performed after an over-
was not possible to hold test Day 2 in the mid-follicular night fast and after at least 15 min of rest in the supine posi-
phase and the second best option was therefore to schedule tion. Resting HR was determined as the lowest value obtained
pre- and post-testing at the same time in the menstrual cycle during the 6 measurements.
but during another menstrual period. These subjects were not
tested in the 2 days before menstruation and at the beginning 2.9. Blood sample collection and analysis
of their menstrual cycle because of fluid accumulation and
general exhaustion. TagedPA 3 mL blood sample diluted with EOTA was collected and
analyzed for basal levels of glycosylated hemoglobin (HbA1c)
using liquid chromatography in a clinical laboratory. Addition-
2.6. Body composition and bone mineral density (BMD)
ally, a 4 mL blood sample diluted with lithium heparin was
TagedPA whole-body DXA scan and a regional DXA scan specific also collected and analyzed for total cholesterol (TC), low-
to the proximal femur were carried out before and after the 12- density lipoprotein cholesterol (LDL-C), high-density lipopro-
week intervention period (iDXA). Total fat percentage, total tein cholesterol (HDL-C), and triglyceride (TG) using auto-
muscle mass, and total fat mass were determined by the mated analyzers (Cobas Fara; Roche, Neuilly sur Seine,
whole-body scan, while BMD in the proximal femur was France) with enzymatic kits (Roche Diagnostics, Mannheim,
determined by the regional DXA. Additionally, body height Germany; Tosoh G7, Tosoh Europe, Tessenderlo, Belgium).
and body weight were measured before the 2 DXA scans. A proportion of the blood samples was collected in 2 mL
Fitness and health benefits of women’s team handball 143

TagedPsyringes diluted with 30 mL Ethylene-bis(oxyethylenenitrilo) TagedPthreshold was not met by the WBQ depression subscale (Cron-
tetraacetic acid (EGTA) and centrifuged for 2 min, after which bach’s a values of 0.37 at pre, and 0.65 at post) and the SMS
the plasma was pipetted and frozen at ¡80˚C for later analysis demotivation subscale (0.50 and 0.90), so it was decided not to
of the plasma concentrations of biomechanical bone turnover proceed with analyzing them. The WBQ subscales of anxiety
markers such as osteocalcin, procollagen type-1 amino-termi- (0.79 and 0.66), energy (0.75 and 0.82) and well-being (0.80
nal propeptide (P1NP), and carboxy-terminal type-1 collagen and 0.90), as well as the SMS subscales of intrinsic motivation
crosslinks (CTX-1), which were evaluated by a fully auto- to know (0.72 and 0.91), intrinsic motivation to accomplish
mated immunoassay system (iSYS; Immunodianostic Systems (0.77 and 0.85), intrinsic motivation to experience stimulation
Ltd., Bolintensdon, UK) using a chemiluminescence method. (0.63 and 0.78), extrinsic motivation—identified regulation
(0.71 and 0.65), extrinsic motivation—introjected regulation
2.10. Intensity and time
motion during training (0.87 and 0.84), and extrinsic motivation—external regulation
(0.86 and 0.93) met the threshold value of 0.60 and it was
TagedPIn HG, HR was recorded at 1 s intervals using an HR moni-
decided to proceed with analyzing them.
tor (Polar Team System) during Weeks 3, 8, and 11. Data
were transferred to a computer for subsequent analysis using
2.12. Statistics
the Polar Team2 system software (Polar Electro Oy). Data are
expressed in relation to individual HRmax. Individual HRmax TagedPPotential between-group differences in baseline measure-
was determined as the highest value reached during either the ments were tested with an unpaired t test. Between- and
incremental treadmill test to exhaustion, the Yo-Yo IE1 test, within-group changes after 12 weeks for HG and CG were
or training. Two training sessions consisting of 4 £ 10 min analyzed using a two-way repeated-measures analysis of vari-
periods of 4 v 4 matches were videoed for later time
motion ance (ANOVA). When a significant time £ group interaction
analysis. The digital video cameras (GR-D23E; JVC, Tokyo, was found, Tukey’s honest significance post hoc tests were
Japan) were positioned 15 m from the pitch. The videos were conducted. All ANOVA main effects (time effect, group
later replayed on a monitor for computerized coding of the effect, and time £ group effect) are presented in the right-hand
activity pattern. Locomotor categories applied in previously column of Table 1. All data are presented as means § SD. Sta-
studies23,24 was also used in this study: standing (0 km/h); tistical analyses were performed using SigmaPlot, Version
walking and jogging (0
6 km/h); moderate-intensity running 11.0 (Systat Software, Inc., San Jose, CA, USA). For all com-
(>6
13 km/h); high-intensity running (>13 km/h); sidesteps parisons, significance was set at p value <0.05.
(>0 km/h); and backwards running (>0 km/h). The time taken
for the player to pass markers on the pitch was used to calcu- 3. Results
late the speed for each locomotion activity. The frequency of
3.1. Body composition
specific actions, such as jumps, turns, throws, catches, and
shots, was also noted during the 40 min total playing time. TagedPA time £ group interaction (p = 0.024) in favor of HG was
observed for total muscle mass from 0 to 12 weeks. In HG,
2.11. Questionnaires total muscle mass was 2.1% higher (p < 0.001) after 12 weeks,
corresponding to an increase in muscle mass of 0.9 kg from
TagedPA Danish version of the Well-Being Questionnaire (WBQ)
43.5 § 4.3 kg to 44.4 § 4.5 kg. In CG, total muscle mass did
was used to assess the participants’ quality of life.25 It com-
not change (p > 0.05). No time £ group interaction (p = 0.072,
prises 22 items measuring 4 subscales of well-being, namely
p = 0.176) was observed for total fat percentage and total fat
anxiety (6 items), depression (6 items), energy (4 items), and
mass from 0 to 12 weeks, even though total fat percentage
positive well-being (6 items). The questions have a Likert-
decreased (p < 0.05) over time in HG from 36.4% § 4.9% to
scale answering format from 0 (not at all) to 3 (all the time).
35.4% § 4.8%. In CG, total fat percentage and total fat mass
When calculating the WBQ subscales, a mean of the 4 or 6
did not change (p > 0.05) (Table 1).
items in a particular scale was calculated.
TagedPA Danish version of the Sport Motivation Scale (SMS) was
3.2. BMD and bone markers
used to measure motivation.26 The SMS measures 3 forms of
intrinsic motivation, namely to accomplish, to experience stimu- TagedPA time £ group interaction (p = 0.041) was observed in
lation, and to know, and 3 forms of extrinsic motivation, namely favor of HG for proximal femur BMD from 0 to 12 weeks. In
identified, introjected, and external regulation. Additionally, HG, proximal femur BMD was 0.8% higher (p < 0.05) after
demotivation was assessed. Each of these subscales is measured 12 weeks, corresponding to an increase of 0.008 g/cm2 from
with 4 items, giving a total of 28 questions, using a Likert-scale 1.084 § 0.094 g/cm2 to 1.092 § 0.087 g/cm2. Furthermore, a
answering format from 1 (Does not correspond at all) to 7 (Cor- time £ group interaction (p < 0.001) was observed in favor of
responds exactly). When calculating the SMS subscales, a mean HG for the bone marker osteocalcin from 0 to 12 weeks. In
of the 4 items belonging to a particular scale was calculated. HG, osteocalcin increased (p < 0.001) by 55.8% from
TagedPInternal consistency for the subscales of WBQ and SMS 20.6 § 6.8 mg/L to 32.1 § 8.8 mg/L. In HG, the bone markers
was tested with a Cronbach’s a test using a threshold value of CTX-I and P1NP also increased by 34.2% (p < 0.05) and
0.60 given the low number of respondents (n = 25) and rela- 32.8% (p < 0.05), respectively, but there was no time £ group
tively low number of items in each subscale (4 or 6). This interaction (p = 0.063, p = 0.083) from 0 to 12 weeks. In CG,
144 T. Hornstrup et al.

TagedPproximal femur BMD, osteocalcin, CTX-I, and PINP did not

change (p > 0.05) (Table 1).

3.3. Performance and VO2peak

TagedPA time £ group interaction (p < 0.001, p = 0.003) was
observed in favor of HG for Yo-Yo IE1 performance and TTE
test performance from 0 to 12 weeks. In HG, Yo-Yo IE1 per-
formance increased (p < 0.001) by 35.3% after 12 weeks from
1073 § 318 m to 1452 § 397 m. Furthermore, HG increased
(p < 0.01) TTE in the treadmill test by 11.5% from 393 § 70 s
to 438 § 64 s. No time £ group interaction (p = 0.124,
p = 0.056) was observed for VO2peak and for HR at 8 km/h
from 0 to 12 weeks, even though HR at 8 km/h decreased
(p < 0.05) in HG from 169 § 12 bpm to 158 § 8 bpm. In CG,
Yo-Yo IE1 performance, TTE, VO2peak, and HR at 8 km/h did
not change (p > 0.05) (Table 1).

3.4. Blood pressure, plasma cholesterol, and HbA1c

TagedPNo time £ group interaction (p > 0.05) was observed for sys-
tolic blood pressure, diastolic blood pressure, mean arterial pres-
sure, resting HR, TC, HDL-C, LDL-C, TG, and HbA1c from 0
to 12 weeks. Furthermore, there was no development over time
for any of the variables in HG and CG (p > 0.05) (Table 1).

3.5. Intensity and time
motion during training

TagedPMean HR during the four 10 min exercise periods during the
handball training was 172 § 14 bpm (mean § SD), correspond-
ing to 85% § 6% of HRmax. Time spent in HR zones corre-
sponding to <70.0%, 70.0%
79.9%, 80.0%
89.9%, 90.0%

94.9%, and 95.0%
100.0% HRmax was 5% § 9%, 13% § 15%,
47% § 14%, 32% § 17%, and 3% § 3% of total playing time,
respectively (Fig. 2A). Time for standing, walking, moderate-
intensity running, high-speed running, sidesteps, and backwards
running accounted for 37.1% § 5.2%, 21.6% § 6.7%,
19.3% § 3.9%, 4.9% § 2.2%, 10.5% § 4.7%, and 7.0% § 4.1%
of total playing time, respectively (Fig. 2B). The frequency of
specific actions such as jumps, turns, catches, throws, and shots
was 8.5 § 3.3, 5.5 § 1.3, 35.7 § 8.1, 28.5 § 9.4, and 7.2 § 2.9
during a 10 min match, respectively (Fig. 2C).
Fig. 2. (A) Heart rate distribution; (B) activity pattern in various locomotor
3.6. Well-being modes: standing (S), walking (W), moderate-speed running (MS), high-speed
running (HS), sidesteps (St), and backwards running (BR); and (C) numbers of
TagedPA time £ group interaction (p = 0.01) was observed in favor actions: jumps, turns, catches, throws, and shots. Mean § SD are presented.
of HG for the energy subscale from 0 to 12 weeks. In HG,
energy was 14.6% (p < 0.05) higher after 12 weeks. In CG, TagedP21.0% (p < 0.001) higher after 12 weeks. In CG, intrinsic
energy did not change (p > 0.05). No time £ group interaction motivation to accomplish was 24.2% (p < 0.01) lower after 12
was observed for the subscales of anxiety or positive well- weeks. A time £ group interaction (p = 0.003) was observed
being from 0 to 12 weeks (p = 0.112 and p = 0.225). There was for intrinsic motivation to know from 0 to 12 weeks. In HG,
no development over time for any of the variables in HG and intrinsic motivation to know tended to be higher (p < 0.1) after
CG (Table 1). 12 weeks. In CG, intrinsic motivation to know was 23.2%
(p < 0.01) lower after 12 weeks (Table 1).
3.7. Motivation
4. Discussion
T time £ group interaction (p < 0.001) was observed in
agedPA
favor of HG for intrinsic motivation to accomplish from 0 to TagedPThe major findings of the present study were that 12 weeks
12 weeks. In HG, intrinsic motivation to accomplish was of recreational handball training performed as small-sided
Fitness and health benefits of women’s team handball 145

TagedPgames led to improvements in total muscle mass and osteo- TagedPwith HRs over 90%HRmax. It is surprising that the present
genic response for the proximal femur, supported by a pro- study was not able to detect an improvement in VO2peak given
nounced rise in the bone marker osteocalcin. Furthermore, that physical performance was markedly improved. VO2peak
physical performance evaluated by Yo-Yo IE1 and incremen- seems to be important for intermittent exercise performance
tal treadmill tests increased markedly in HG. HG also and incremental treadmill test performance in untrained indi-
increased in the well-being subscale of energy and the motiva- viduals, whereas the anaerobic energy system seems more
tion subscales of intrinsic motivation to accomplish and to important in trained individuals.35 VO2peak has a major influ-
know after 12 weeks of recreational handball. ence on mortality and is therefore an important parameter
when it comes to preventing lifestyle diseases.36
38 A similar
4.1. Body composition and osteogenic response study of young men already conducted in our laboratory (data
not published) showed that VO2peak was increased by 11%
TagedPThe increase in total muscle mass of 0.9 kg in HG is in
(p < 0.05) after 12 weeks of recreational handball.
agreement with data from previous studies of recreational
football in men and premenopausal women.8,27 Higher muscle
mass (relative to body size) is associated with health benefits 4.3. Cardiovascular risk factors
such as better insulin sensitivity and lower risk of developing
TagedPPositive effects on cardiovascular risk factors such as sys-
prediabetes mellitus.28 Along with the increase in muscle
tolic and diastolic blood pressure, resting HR, TC, LDL-C and
mass, total fat mass tended to fall. This combination is of
HDL-C, and TG concentrations were not observed after 12
major importance for lowering the risk of developing T2DM
weeks of small-sided handball. Systolic and diastolic blood
and cardiovascular disease.
pressure were low for both groups before the intervention
TagedPAnother important finding was that 12 weeks of small-sided
period, with values of 103/69 and 103/67 mmHg, respectively
handball increased BMD in the proximal femur by 0.8%. We
(Table 1). The lack of effect on blood pressure accords with
chose to evaluate changes in the proximal femur because this
observations in football studies for those groups who had low
area, together with the spine, is where osteoporosis is diag-
baseline values.14 The resting HR in HG and CG was at the
nosed. One third of all women will at some time have a frac-
lower end of the curve compared to the general population39
ture in the proximal femur or the spine, and the prevalence of
before the intervention period, with values of 59 and 61 bpm,
osteoporosis is continuing to escalate worldwide.29 Interven-
respectively. Nevertheless, several intervention studies, includ-
tion studies, including football studies,8,12,30 have failed to
ing football studies, demonstrated positive effects. In a study
demonstrate a short-term training response in total BMD and
by Krustrup et al.,27 it was observed that recreational football
leg BMD when BMD is evaluated by whole-body DXA scans.
in premenopausal women lowered resting HR from 62 to
When a regional DXA scans for the proximal femur or pQCT
57 bpm after 16 weeks of training. Taking into account that the
scans of tibia have been performed, an increased BMD, has
subjects in HG had a high intensity of 85%HRmax during train-
been observed in middle-aged women after 15 weeks of recre-
ing and for 30% of the time worked at intensities above 90%
ational football.30 It seems that the method used is of major
HRmax, it seems reasonable to suggest that the lack of effect on
importance in detecting training-induced changes in BMD.
resting HR was due to the subjects’ relatively high VO2peak at
The osteogenic response in HG was supported by a 55.8%
baseline combined with the lower attendance rate (1.7 times
increase in the bone formation marker osteocalcin. The bone
per week) compared to the study by Krustrup and colleagues.27
formation marker P1NP and bone resorption marker CTX-1
TagedPAs with the other cardiovascular risk factors, there was no
were not significantly changed compared to CG.
effect on lipid profile after 12 weeks of small-sided handball.
TagedPTime
motion analysis in the present study shows that rec-
The subjects in the study had no indication of dyslipidemia,
reational handball, like recreational football, involves multiple
which could explain the absence of effects. Aerobic exercise,
intense movements in different directions (high-speed running,
however, is associated with beneficial effects on TC, HDL-C,
accelerations, decelerations, jumps, turns, and rapid side-cut-
and TG concentrations.40 According to Bozetto et al.,41 lipid-
ting movements), which seems of major importance for the
lowering effects of a training program are related to the
adaptations in muscle mass and the osteogenic response after
amount of energy expended, which is determined by the dura-
12 weeks of training. This is in agreement with studies that
tion and intensity of the physical activity. As mentioned ear-
have observed that positive bone formation stimuli depend on
lier, the intensity observed during training in HG was high.
the presence of high mechanical impact, which may be evoked
However, the attendance rate was lower compared to studies
by large ground reaction and muscle forces exerted on the
with recreational football, which have seen an effect on
bone tissue at a high strain rate and in varied directions in rela-
LDL-C and LDL/HDL ratio.8,22 In a study by de Sousa
tion to the longitudinal bone axis.31
34
et al.,42 an even more pronounced effect on lipid profile in
terms of TC, LDL-C, LDL/HDL ratio, and TG was observed
4.2. VO2max and physical performance
when the 12 weeks of recreational football training was com-
TagedPYo-Yo IE1 performance and TTE during treadmill testing bined with a calorie-restricted diet. In the present study, we
increased markedly in HG, by 35.3% and 11.5%, respectively. did not control the subjects’ dietary intake but recommended
The time
motion analyses in the present study show that rec- that they continued their normal lifestyle patterns. Changes in
reational handball is an intermittent high-speed exercise mode diet affect lipid concentration in plasma after just 3 days43 and
146 T. Hornstrup et al.

TagedPit seems that the lack of effect on lipid profile may be due to a TagedPstudy. Elite Handball has shown to have an injury rate of 1.5
combination of the lower attendance rate compared to other per match of which 84% of the injuries is caused by body-con-
studies in healthy untrained subjects and possible changes in tact.46 There are no previous studies that have shown the inci-
diet during the intervention study. dence of injuries in recreational handball. The rules applied in
the present study did not allow hard tackles, thus it was
4.4. Well-being expected that the injury rate would be far less than in elite
handball. Furthermore, it is observed that the risk of injuries in
TagedPPositive effects on mental energy were observed after 12 recreational football is 5
10 times less when playing small-
weeks of small-sided handball. This confirms earlier findings sided football compared to 11 v 11 match-play.9 At last the
that participation in physical activity increases feelings of warm-up was composed of a well-organized program inspired
energy.44 Effects on anxiety and positive well-being were not from FIFA 11+, which had been shown to be associated with a
observed. This is in contrast with earlier studies, in which posi- 30
50 percent reduction of injuries.10,11 Therefore, recrea-
tive effects on a variety of well-being parameters were tional handball, does not give cause for concern when it comes
found.44,45 However, this group of young women had low lev- to injuries, but future large-scale studies are warranted to
els of anxiety (ranging from 1.40 to 1.74 on a scale from 1 to investigate this further.
4), making it possible that the intervention could not provide
further improvements. Finally, looking at the positive well-
5. Conclusion
being subscale, the values seem to be centered around 3 (rang-
ing from 2.93 to 3.14) on a scale from 1 to 4, making it TagedPFor young women, recreational handball represents inter-
unlikely that there is a ceiling effect. mittent high-intensity exercise that leads to beneficial effects
on bone mineral density, muscle mass, and performance. It
4.5. Motivation will be interesting to observe whether future studies in other
populations are able to detect changes in cardiovascular fit-
TagedPThe handball group experienced better development in ness, which the present study failed to demonstrate. The young
intrinsic motivation to accomplish and to know. The effects women in the present study had a superior cardiovascular risk
were brought on by an increase in motivation in HG (albeit profile, which may partly explain why there were no changes
p = 0.071 for intrinsic motivation to know) as well as a in cardiovascular fitness. In addition, recreational handball
decrease in motivation in CG (significant for both intrinsic seems to increase some aspects of well-being and motivation.
motivation to accomplish and to know). For intrinsic motiva- These results can likely be applied to other populations,
tion to experience stimulation, similar tendencies were found, though it remains to be investigated whether the results would
but they were not significant. Taken together with the results be even better in sample populations with a more vulnerable
for intrinsic motivation to accomplish and to know, it seems psychological profile.
plausible that an effect on intrinsic motivation to experience
stimulation could be found given a larger sample. Overall, it
Acknowledgments
seems that 12 weeks of small-sided handball, compared with
inactivity, increases intrinsic motivation. This is in line with TagedPWe would like to thank all the participants for their com-
earlier studies in which it was found that team games were mitted participation. We would also like to thank Jens Jung
intrinsically motivating for previously inactive participants.15,16 Nielsen, Jon Egelund, Mikkel Alleslev Larsen, Frederik Ter-
These studies have highlighted that team games are particu- kildsen Løwenstein, Christian Ritz, and Nadia Quardon for
larly motivating because they have a high degree of social their excellent practical and technical assistance. A special
interaction and technical and tactical challenges, and therefore thank goes to TrygFonden, Nordea-fonden (Nordea-fonden
satisfy basic psychological needs for competence and related- 02-2011-4360) and the Danish Handball Federation (Dansk

ness, which in turn increases intrinsic motivation. It seems Handbold Forbund, DHF), as well as the European Handball
likely that there is a similar effect for the handball activities in Federation (EHF) for their collaboration and financial support.
this study. No effects were observed for any of the extrinsic The authors declare that this study was funded in part by the
motivation subscales. While it is possible that extrinsic motiva- Danish and European Handball Federation.
tion could increase for HG through motives such as better
health, better looks, or feelings of responsibility toward other Authors’ contributions
participants or oneself, this result was not obtained in this
study. Nielsen et al.15 and Pedersen et al.19 mentioned that an TagedPTH conceived of the study design, applied for funding, con-
increase in intrinsic motivation takes precedence over any pos- ducted the training and testing, carried out the data collection
sible increases in extrinsic motivation, making the results of and analysis, interpreted the study results, and drafted the man-
this study plausible and in corroboration of existing research. uscript; JMW conceived of the psycho-social part of the study
design, carried out data collection, interpreted the study
results, and edited the manuscript; BF conducted the training,
4.6. Injuries
carried out the video analysis, and edited the manuscript; SP,
TagedPIn the present study, no severe injuries were observed. One EWH, JWH, JLA, and LN contributed the study design, inter-
participant had an ankle sprain and dropped out from the preted the study results, and edited the manuscript; SHN
Fitness and health benefits of women’s team handball 147

TagedPconducted the training, carried out the analysis of HR data, and TagedP17. Povoas SCA, Castagna C, Resende C, Coelho EF, Silva P, Santos R, et al.
edited the manuscript; PK conceived of the study design, Physical and physiological demands of recreational team handball for
applied for funding, interpreted the study results, and edited adult untrained men. Biomed Res Int 2017;2017. doi: 10.1155/2017/
6204603
the manuscript. All authors have read and approved the final TagedP18. Randers MB, Hagman M, Brix J, Christensen JF, Nielsen JJ, Pedersen
version of the manuscript, and agree with the order of presen- MT, et al. Effects of 3 months of full-court and half-court street basketball
tation of the authors. training on health profile in untrained men. J Sport Health Sci
2018;7:132–8.
TagedP19. Pedersen MT, Vorup J, Nistrup A, Wikman JM, Alstrøm JM, Melcher PS,
Competing interests et al. Effect of team sports and resistance training on physical function,
quality of life, and motivation in older adults. Scand J Med Sci Sports
TagedPThe authors declare that they have no competing interests. 2017;27:852–64.
TagedP20. Deci EL, Ryan RM. Intrinsic motivation and self-determination in human
behavior. New York, NY: Plenum Publishing; 1985.
TagedP21. Ryan RM, Deci EL. Self-determination theory and the facilitation of
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