Color Beta Carotene 2009 TR
Color Beta Carotene 2009 TR
Color Beta Carotene 2009 TR
Handling/Processing
1
2 Identification of Petitioned Substance
3
4 Chemical Name: CAS Number:
5 β-Carotene; Β-Carotene 7235-40-7
6
7 Other Names: Other Codes:
8 β,β-Carotene; Carotaben; Provatene; Solatene; EINECS No. 230-636-6
9 all-trans-β-Carotene INS1 No. 160a(ii)
10
11 Characterization of Petitioned Substance
12
13 Composition of the Substance:
14
15 Carotenoids are natural pigments, which are synthesized by plants and are responsible for the bright colors of
16 various fruits and vegetables. They act as photosynthesis aids and for the photo protection of their hosts (Isler
17 1971; Britton and others 1995). There are several dozen carotenoids in the foods that we eat, and most of these
18 carotenoids have antioxidant activity. β-Carotene is the most common carotenoid consisting of a highly
19 branched, unsaturated chain containing identical substituted ring structures at each end; see Figure 1.
20
21
22
23 Figure 1. β-Carotene Chemical Structure
24
25 It is made of two molecules of retinol2 (an alcohol), Figure 2 (see below), and possesses maximal provitamin A
26 activity (Ball, 1996). Retinol can only be found in animal sources and can be converted by the body into retinal
27 (an aldehyde) and retinoic acid (a carboxylic acid), other active forms of vitamin A.
28
29
30
31 Figure 2. Retinol Chemical Structure
32
33 Carotene was first isolated from carrots by Wackenroder in 1831 (Davies, 1976). It is a general term describing
34 certain polyene hydrocarbons containing 40 carbon atoms. Three of these, α-, β- , and γ-carotene, as well as some
35 closely related oxygen-containing carotenoids, exhibit provitamin A activity (SCOGS Report No. 111). The
36 petitioned substance has the molecular formula C40H56 and is comprised of 89.49% C and 10.51% H (Merck Index,
37 2006).
38
39 In plants, β-carotene occurs almost always together with chlorophyll (Merck Index, 2006). It is the major coloring
40 principle in carrot and as well palm oil seed extracts. In addition, β-carotene is found in cantaloupe, apricots,
41 sweet potatoes, pumpkin, winter squash, mangos, collard greens, spinach, kale, broccoli, and other orange, red,
42 and dark green fruits and vegetables.
43
44 Properties of the Substance:
45
46 The petitioned substance occurs as red crystals or crystalline powder (FCC, 2010-2011). The absorption
47 spectrum of β-carotene shows between 400-500 nm, which is the green/blue part of the spectrum (Isler,
48 1971). Therefore, the molecule of β-carotene absorbs green/blue lights and gives off red/yellow colors.
49
50 The petitioned substance is insoluble in water, acids and alkalies, but is soluble in carbon disulfide and
51 chloroform. β-Carotene is practically insoluble in methanol and ethanol, and is sparingly soluble in ether,
52 hexane, and oils (FCC, 2010-2011). The diluted solution is yellow. It absorbs oxygen from the air giving
53 rise to inactive, colorless oxidation products (Merck Index, 2006). In other words, β-carotene changes in
54 color from a fairly deep reddish-orange to the oxidized product, which is a light, yellowish gray (Furia,
55 1972). β-Carotene melts between 176° C and 182° C, with decomposition. Its molecular weight is 536.87
56 g/mol (Merck Index, 2006; FCC, 2010-2011).
57
58 The best characterized natural functions of carotenoids (including β-carotene) are to serve as light-
59 absorbing pigments during photosynthesis and protection of cells against photosensitization (SCF, 2000).
60 In plants, carotenoids have the important antioxidant function of quenching (deactivating) singlet oxygen,
61 an oxidant formed during photosynthesis (Halliwell and Gutteridge, 1999). Although important for plants,
62 the relevance of singlet oxygen quenching to human health is less clear (LPI, 2009).
63
64 Specific Uses of the Substance:
65
66 β-Carotene is a direct human food ingredient which functions as a color additive and a nutrient
67 supplement. This substance is used in dairy products, fats and oils, and processed fruits and fruit juices; it
68 may be used in infant formula as a source of vitamin A in accordance with 21 CFR §184.1254. In order to
69 be traded, the petitioned substance must be formulated in hydrophilic (juices and drinks) or lipophilic
70 (butter, margarine, and cheese) matrices for food industry application (Ribeiro and others, 2011).
71
72 The petitioner stated that β-carotene would be used to color food and beverage products including, but not
73 limited to, yogurts, dairy beverages, ice cream, pudding, confectionery, bakery products, and condiments.
74
75 According to FDA, the color additive β-carotene may be safely used in coloring drugs and cosmetics. It can
76 be applied to an array of animal foods designed for pets, including dogs, cats, fish, and birds (Dufosse and
77 others, 2005).
78
79 Approved Legal Uses of the Substance:
80
81 FDA— In 21 CFR §184.1245, it is stated ―β-carotene (CAS Reg. No. 7235-40-7) has the molecular formula
82 C40H56. It is synthesized by saponification of vitamin A acetate.‖ Furthermore, in Section §73.95, it is stated
83 ―The color additive is β-carotene prepared synthetically or obtained from natural sources.‖ Uses of β-
84 carotene are listed in Table 1, see below.
85
86 Table 1. FDA Regulations, 21 CFR
87
Regulatory Citations Status Use Limits
SUBCHAPTER B— Part 184—Direct Food
FOOD FOR Substances Affirmed
HUMAN As Generally
CONSUMPTION Recognized As Safe
Subpart E—Listing of
Specific Substances
Affirmed as GRAS
3Vitamin A is a general term for a group of compounds that includes provitamin A carotenoids and
preformed vitamin A.
105 and retinoic acid (other forms of vitamin A). [Note: Common provitamin A carotenoids are α-carotene,
106 β-carotene, and β-cryptoxanthin. Among these, β-carotene is most efficiently made into retinol; α-
107 carotene and β-cryptoxanthin are also converted to vitamin A, but only half as efficiently as β-carotene
108 (IMO, 2001).] Retinol and retinal can be reversibly oxidized and reduced; but retinoic acid cannot be
109 converted back to retinal after it has been formed.
110
111 Absorbed β-carotene is principally converted to vitamin A by the enzyme β-carotene-15,15′-
112 dioxygenase within intestinal absorptive cells (IOM, 2001). The central cleavage of β-carotene by this
113 enzyme will, in theory, result in two molecules of retinal (also called retinaldehyde). β-Carotene can
114 also be cleaved eccentrically to yield β-apocarotenals that can be further degraded to retinal or retinoic
115 acid (Krinsky and others, 1993). The retinal form is required by the eye for the transduction of light
116 into neural signals necessary for vision (Saari, 1994); the retinoic acid form is required to maintain
117 normal differentiation of the cornea and conjunctival membranes, thus preventing xerophthalmia, as
118 well as for the photoreceptor rod and cone cells of the retina (IOM, 2001). In addition, vitamin A plays
119 an important role in bone growth, reproduction, immunity, cell development, and skin health (NIH,
120 2006).
121
122 Vitamin A in foods that come from animals can be well absorbed and used efficiently by the body.
123 However, vitamin A in foods that come from plants cannot be as well absorbed as animal sources of
124 Vitamin A.
125
126 Status
127
128 Domestic:
129
130 EPA — Neither List 4A (Minimal Risk Inert Ingredients – By Chemical Name) nor List 4B (Other ingredients for
131 which EPA has sufficient information to reasonably conclude that the current used pattern in pesticide products will
132 not adversely affect public health or the environment – By Chemical Name) contains β-carotene. Lists 4A and 4B
133 were updated by August 2004. However, ―.beta.,.beta.-Carotene; CAS No. 7235-40-7‖ is listed in EPA
134 Substance Registry Services, updated on June 16, 2011.
135
136 FDA — β-Carotene is affirmed as GRAS, see Table 1 in the Approved Legal Uses of the Substance section,
137 in 1979. The petitioned substance may be used as a nutrient supplement or a color additive. β-Carotene
138 may be the subject of an antioxidant nutrient content claim on food labeling (21 CFR §101.54(g)(3)).
139 According to 21 CFR §73.95 (e), certification of this color additive is not necessary for the protection of the
140 public health and therefore batches thereof are exempt from the certification requirements.
141
142 International:
143
144 Codex —In the food additive groups listed on Table One (Additives Permitted for Use Under Specified
145 Conditions in Certain Food Categories or Individual Food Items) of Codex General Standard for Food Additives:
146 ―INS 160a(ii) β-Carotenes (vegetable)‖ is under ―CAROTENES, Β-(VEGETABLE)‖; ―INS 160a(i) β-
147 Carotenes (synthetic)‖ and ―INS 160a(iii) β-Carotenes (Blakeslea trispora)‖ are under ―CAROTENOIDS‖.
148 They are classified as color. β-Carotene can be used in dairy, fruit and vegetable, fish and processed meat,
149 baked, and confectionery products. This standard was revised in 2010.
150
151 European Union — ―E 160a(ii) Β-CAROTENE‖ is listed under ANNEX of COMMISSION DIRECTIVE
152 2004/47/EC of 16 April 2004 amending Directive 94/45/EC as regards mixed carotenes (E 160a (i)) and β-carotene (E
153 160a (ii)). Function as colors for use in foodstuffs (Directive 94/36/EC). [Note: ―E 160a(i) MIXED
4Provitamin A carotenoids are found in foods that come from plants including oily fruits and red palm oil.
5Retinol, also called preformed vitamin A, is found in foods that come from animals, including beef liver,
whole eggs, whole milk, margarine, and some fortified food products such as breakfast cereals.
154 CAROTENES‖ include plant and algal carotenes; ―E 160a(ii) Β-CAROTENE‖ include β-carotene and β-
155 carotene from Blakeslea trispora.]
156
157 Canada — ―β-Carotene‖ is included in Natural Health Products Ingredients Database. Purposes: color
158 additive. ―Carotene‖ is also under Food Additives Permitted for Use in Canada. On March 25, 2011, Canadian
159 Food Inspection Agency proposed amendments to the Feeds Regulations. ―β-carotene‖ is listed on Class 7
160 (Vitamin Products and Yeast Products) of Schedule IV (Part II) of the proposed updated version.
161
162 Japan — ―β-Carotene (72)‖ is listed on Table 1 related to Articles 12 and 21 of the Food Sanitation Law
163 Enforcement Regulations by Japan Ministry of Health, Labor, and Welfare (MHLW). Last amendment as
164 of July 26, 2005. In addition, ―β-Carotene‖ and ―Carrot carotene, a substance composed mainly of carotene
165 obtained from carrot roots‖ are appeared in List of Designated Additives and List of Existing Food Additives6,
166 respectively, under MHLW Food Additives.
167
168 IFOAM — Not listed.
169
170 Evaluation Questions for Substances to be used in Organic Handling
171
172 Evaluation Question #1: Discuss whether the petitioned substance is formulated or manufactured by a
173 chemical process, or created by naturally occurring biological processes (7 U.S.C. § 6502 (21)).
174
175 The petitioned substance can be manufactured by chemical synthesis; biological synthesis using
176 microorganisms or algae; or through extraction from plant sources.
177
178 (A) Chemical Synthesis
179
180 Synthetic β-carotene has been produced by Roche since 1954 and BASF since 1960. Each company uses a
181 different method for its production; however, both companies utilize the same precursor, β-ionone, which
182 was originally obtained by the condensation of acetone with citral (Russell and Kenyon, 1943). The sources
183 of citral, a C-10 unsaturated aldehyde, were lemon grass oil or turpentine from pine, which are natural
184 products. [Note: Natural lemon grass oil varies widely in purity, availability, and price.] However, β-
185 ionone is now produced from acetone or butadiene (Isler 1971; Britton et al. 1996).
186
187 The Roche production method of β-carotene is the first industrial synthesis (based on enol-ether
188 condensation, i.e. the Grignard reaction), followed the C19+C2+C19 synthesis principle. The chain
189 lengthening proceeds in three steps: (1) acetal formation, (2) Lewis acid-catalyzed insertion of the enol-
190 ether, (3) hydrolysis of the acetal and elimination of alcohol (Britton and others, 1996).
191
192 The BASF production method of β-carotene is based on the Wittig condensation (Wittig reaction), followed
193 the C20+C20 synthesis principle. It starts with phosphonium salts reacting with an aldehyde, generating a
194 double bond and enlarging the polyenic chain. During the reaction, vitamin A acetate is formed which can
195 be used as a starting material for the preparation of carotenoids.
196
197 The synthesis process of β-carotene from Roche presents a yield of 60%, while the process used by BASF
198 presents a yield of 85%. However, the BASF method, based on the Wittig reaction, requires
199 triphenylphosphine oxide recycling, due to its low biodegradability (Isler 1971; Britton and others, 1996).
200
201 BASF can also produce 99.9% pure, crystalline β-carotene, but it does not sell it in this form (US Court of
202 International Trade Reports, 2005). For example: In the production of Lucaroltin® 1% (a food colorant), it
203 takes the synthetic β-carotene crystals and disperses them in vegetable oil with heat, making it into a
6The substances that were already marketed or used on the date of the amendment of the Food Sanitation
Law and appear in the List of Existing Food Additives
204 solution. This solution mixes with another solution containing sugars and dextrin, then vitamin
205 emulsifiers in the ester form and ascorbyl palmitate are added (US Court of International Trade Reports,
206 2005).
207
208 (B) Biological Production Methods
209
210 β-Carotene from microorganisms (fungi, yeasts, or bacteria)
211
212 β-Carotene can be produced by filamentous fungi, such as Blakeslea trispora and Phycomyces
213 blakesleeanus, which also generate ubiquinone, ergosterol, organic acids, and others carotenoids like
214 lycopene, γ-carotene, and phytoene (Ribeiro and others, 2011). According to JECFA specification
215 (2007), β-carotene is produced by a fermentation process using the two sexual mating types (+) and (–)
216 of the fungus Blakeslea trispora. β-Carotene is then isolated from the biomass by solvent extraction and
217 crystallized. The coloring principle consists predominantly of trans-β-carotene together with variable
218 amounts of cis isomers of β-carotene. The solvents used in the extraction and purification are ethanol,
219 isopropanol, ethyl acetate, and isobutyl acetate.
220
221 Some yeast species (such as Rhodotorula glutinis, R. minuta, R. mucilaginosa, and R. graminis) can also be
222 used for the production of carotenoids. R. glutinis is able to grow in various agricultural raw materials
223 (such as sugar cane juice, peat extract, whey, grape must, beet molasses, hydrolyzed mung bean waste
224 flour, soybean and corn flour extracts and sugar cane molasses) for carotenoid production. Depending
225 on the growing conditions, such as carbon and nitrogen sources, R. glutinis may produce carotenoid
226 mixtures with profiles quite variable, but in general β-carotene is the main product (Ribeiro and others,
227 2011).
228
229 Among bacteria, some carotenogenic species can produce β-carotene as the main carotenoid. They
230 must have the central metabolism inhibited by inorganic salts and urea, as in the case of Flavobacterium
231 multivorum (Ribeiro and others, 2011).
232
233 β-Carotene from algae
234
235 Algae are a group of non-vascular plants which are autotrophic and are able to harness solar energy.
236 They account for the largest quantities of biomass accumulation through the photosynthesis
237 mechanism (Dufosse and others, 2005). The genus Dunaliella is one of the most reported for the
238 production of carotenoids and belongs to the group of halotolerant unicellular microalgae. Species
239 from this genus can accumulate large amounts of β-carotene in chloroplasts when high luminous
240 intensity is obtained (Ribeiro and others, 2011). Commonly cultivated species are D. salina and D.
241 bardawil (Dufosse and others, 2005).
242
243 Carotenes are obtained by solvent extraction of the dried Dunaliella. The solvents used for the
244 extraction are carbon dioxide, acetone, methanol, propan-2-ol, hexane, ethanol, and vegetable oil. The
245 main coloring principles are trans and cis-β-carotene together with minor amounts of other carotenoids
246 such as α-carotene and xanthophyll. Besides the color pigments, carotenes may contain lipids,
247 naturally occurring in the source material, food grade vegetable oil, and tocopherol added to retard
248 oxidation of the pigment (JECFA specification, 2007).
249
250 According to the petition, β-carotene is produced from natural strains of the algae D. salina, an algae
251 grown in large saline lakes located in Whyalla, South Australia. It is extracted from the algae using
252 carbon dioxide, ethanol, or vegetable oil. No less than 96% total extracted coloring matters will be in
253 the form of β-carotene.
254
255 (C) Extraction from Plant (Vegetable)
256
257 β-Carotene from vegetables is derived from solvent extraction of carrots, oil of palm fruit, sweet potato,
258 and other edible plants with subsequent purification. The solvents used for the extraction include hexane,
259 acetone, ethyl acetate, ethanol, and ethyl lactate (Ribeiro and others, 2011). The main coloring principles
260 are α- and β-carotenes of which β-carotene accounts for the major part. Besides the color pigments, these
261 substances may contain oils, fats and waxes naturally occurring in the source material (JECFA
262 specification, 2006).
263
264 Although β-carotenes obtained from both synthetic chemicals and natural sources (such as fungi, algae, or
265 plant) have the same molecular polyenic structure, the β-carotenes made from natural sources contain
266 several other carotenoids in low concentrations (Ribeiro and others, 2011).
267
268 (D) Other Methodology
269
270 Nowadays, combinatorial genetic engineering is being addressed, based on an increasing number of
271 known carotenogenic gene sequences (Mijts and others, 2005). According to the review reported by
272 Dufosse and others (2005), it is stated ―Research projects mixing molecular biology and pigments were
273 investigated all over the world and it seems that current productions are not effective in terms of final
274 yield.‖
275
276 Evaluation Question #2: Describe the most prevalent processes used to manufacture or formulate the
277 petitioned substance. Further, describe any chemical change that may occur during manufacture or
278 formulation of the petitioned substance when this substance is extracted from naturally occurring plant,
279 animal, or mineral sources. (7 U.S.C. § 6502 (21))
280
281 As described in Evaluation Question (EQ) #1, the petitioned substance can be made from synthetic
282 chemicals, or made from natural sources using microorganisms or algae or is extracted from plants. The
283 most prevalent processes are as follows:
284
285 (A) Chemical Synthesis
286
287 The majority of β-carotene commercialized in the world is by chemical synthesis from β-ionone (Raja and
288 others, 2007; Ribeiro and others, 2011). β-ionone was originally synthesized from natural resources, such as
289 lemon grass oil or turpentine from pine, but currently β-ionone is produced from acetone or butadiene.
290 According to 21CFR 184.1245 (a), β-carotene is synthesized by saponification of vitamin A acetate. It stated
291 ―The resulting alcohol is either reacted to form vitamin A Wittig reagent or oxidized to vitamin A
292 aldehyde. Vitamin A Wittig reagent and vitamin A aldehyde are reacted together to form β-carotene.‖
293
294 The synthetic product is predominantly all trans isomers of β-carotene together with minor amounts of
295 other carotenoids; diluted and stabilized forms (including solutions or suspensions of β-carotene in edible
296 fats or oils, emulsions and water dispersible powders) are prepared from β-carotene (JECFA specification,
297 2006).
298
299 (B) Biological Production Processes
300
301 According to Echavarri-Erasun and Johnson (2002), fungi and microalgae appear most promising for
302 industrial production of carotenoids.
303
304 β-Carotene from filamentous fungi (Blakeslea trispora)
305
306 The source organism, the mold Blakeslea trispora, is a plant commensal of tropical plants, some strains
307 of which produce high levels of β-carotene. The fungus exists in (+) and (–) mating type, of which the
308 (+) type synthesizes trisporic acid, a precursor of β-carotene. Mating the two types in a specific ratio,
309 the (–) type then produces large amounts of β-carotene. Glucose and corn steep liquor could be used
310 as carbon and nitrogen sources. By-product of cheese manufacture, i.e. whey, has also received
311 consideration, with strains acclimatized to lactose.
312
313 The production process proceeds essentially in two stages (Dufosse, 2006):
314 The initial stage, fermentation process, seed cultures are produced from the original strain cultures
315 and subsequently used in an aerobic submerged batch fermentation to produce a biomass rich in β-
316 carotene.
317 The second stage, the recovery process, the biomass is isolated and transformed into a form
318 suitable for isolating β-carotene, which is extracted from the biomass with ethyl acetate, suitably
319 purified and concentrated, and the β-carotene crystallized from the mother liquor.
320
321 The final product is either crystalline β-carotene (purity>96%) or it is formulated as a 30% micronized
322 suspension in vegetable oil. The production process is controlled by good manufacturing practice
323 procedures, adequate hygiene control, and adequate control of the raw materials (Dufosse, 2006).
324
325 β-Carotene from microalgae (Dunaliella salina)
326
327 According to Browitzka’s report (1998), the halophilic green flagellate, Dunaliella salina, is the best
328 natural source of the carotenoid β-carotene. The processes of commercial production β-carotene by D.
329 salina are as follows (Dufosse and others, 2005; Dufosse, 2006; Oren, 2010):
330 Cultivation ― It is carried out in either extensive cultures in large unstirred outdoor ponds
331 (extensive culture system), or more intensively in paddlewheel stirred raceway ponds (intensive
332 culture system). D. salina is a halotolerant organism which grows in high salt concentration.
333 Essentially the algae require bicarbonate as a source of carbon and other nutrients such as nitrate,
334 sulfate, and phosphate. It can be operated in two stages. First, initial growth phase requires in
335 nitrate rich medium; magnesium salt is essential as it is required for chlorophyll production. In the
336 second stage, nitrate limitation is induced to stimulate carotenogenesis. For the carotenogenesis
337 phase, nitrate depletion along with salinity maintenance and light stress are essential.
338 Harvesting ― For the extensive culture system, flocculation and surface adsorption are used.
339 Flocculants such as alum (aluminum sulfate), ferric chloride, ferric sulfate, lime, or polysaccharides
340 are employed. For the intensive culture system, centrifuges are generally applied (centrifugation
341 using continuous-flow and automatic discharge) to harvest the cells.
342 Drying ― Algal biomass after harvesting can be dehydrated by using freeze–drying, spray-drying,
343 or drum drying.
344 Extraction ― β-Carotene can be isolated from algal biomass or dried powder by using hot edible oil
345 extraction, supercritical carbon dioxide, or other solvents (such as hexane, ethanol, chloroform, and
346 diethyl ether).
347
348 The extracted β-Carotene can be concentrated, crystallized, and a range of different formulations
349 produced, depending on the final application.
350
351 (C) Extraction from Plant (Vegetable)
352
353 For producing β-carotene from plant sources, the classical method is solvent extraction. In a review article
354 reported by Aberoumand (2011), it stated ―Today, only one crystalline carotene preparation extracted from
355 dehydrated carrots is still on the market.‖
356
357 Evaluation Question #3: Provide a list of non-synthetic or natural source(s) of the petitioned substance
358 (7 CFR § 205.600 (b) (1)).
359
360 Natural sources of β-carotene, as so-called natural β-carotene, have been mentioned in EQs #1 & #2.
361 According to Aberoumand’s review report (2011), only one crystalline carotene extracted from dehydrated
362 carrots is still on the market today. However, other vegetable sources have been pointed out as having
363 great potential for the production of β-carotene, shown in Table 2 (Ribeiro and others, 2011).
364
365 Table 2. Potential Vegetable Resources Rich in β-Carotene
366
Vegetable Resources Carotenoids (μg/g) β-Carotene (%)
Carrot (Daucus carota) 85–174 49–65
Palm (oil) (Elaeis guineensis) 470–700 54.4
Sweet potato (Ipomoea batatas) 160–226 92–95
Buriti (fruit) (Mauritia vinifera) 513.9 72.5
Barbados cherry (Malpighia glabra) 8.8–18.8 69.8–90.6
Tucumã (Astrocaryum aculeatum) 62.6–96.6 75.6–89.3
Pajurá (Couepia bracteosa) 17.8 92.1
Piquiá (Caryocar villosum) 21 85.4
Umari (Poraqueiba sericea) 102.9 78.9
367
368 It is estimated that the worldwide market of carotenoids will grow 2.3% per year, reaching USD 920 million
369 in 2015 (BCC Research, 2008; Ribeiro and others, 2011). β-Carotene accounts for 32% of this market, with a
370 global market estimated to surpass USD 280 million in 2015. Only 2% of the total β-carotene produced
371 worldwide is natural and is mainly used as a nutritional supplement (Dufosse and others, 2005; Ribeiro
372 and others, 2011).
373
374 BCC Research’s report on the Global Market for Carotenoids (2008) stated in part:
375 β-Carotene is still the most prominent carotenoid used in foods and supplements, but due to a
376 changing consumer perception, primarily in Europe, the product is suffering from natural
377 replacements, specifically carrot juice, and market growth in the past few years was much lower
378 than expected. In parallel, the number of producers of synthetic and algae derived β-carotene rose
379 sharply, which added to the imbalance of supply and demand, driving prices down…
380
381 Evaluation Question #4: Specify whether the petitioned substance is categorized as generally
382 recognized as safe (GRAS) when used according to FDA’s good manufacturing practices. (7 CFR §
383 205.600 (b)(5))
384
385 The petitioned substance (β-carotene, CAS Reg. No. 7235–40–7) is listed on 21 CFR §184.1245 of Subpart B
386 (Listing of Specific Substances Affirmed as GRAS) of PART 184 (DIRECT FOOD SUBSTANCES AFFIRMED AS
387 GENERALLY RECOGNIZED AS SAFE). In accordance with FDA, the affirmation of β-carotene as GRAS as
388 a direct human food ingredient is based upon the following current good manufacturing practice
389 conditions of use (§184.1245):
390 The ingredient is used as a nutrient supplement as defined in §170.3(o)(20).
391 The ingredient is used in the following foods at levels not to exceed current good manufacturing
392 practice: dairy product analogs as defined in §170.3(n)(10); fats and oils as defined in §170.3(n)(12); and
393 processed fruits and fruit juices as defined in §170.3(n)(35). Β-carotene may be used in infant formula
394 as a source of vitamin A.
395
396 The following are excerpts from 21 CFR Part 170 Food Additives §170.3 Definitions:
397
398 ―§170.3 (o)(20) Nutrient supplements : Substances which are necessary for the body's nutritional and
399 metabolic processes.
400 §170.3 (n)(10) Dairy product analogs, including nondairy milk, frozen or liquid creamers, coffee
401 whiteners, toppings, and other nondairy products.
402 §170.3 (n)(12) Fats and oils, including margarine, dressings for salads, butter, salad oils, shortenings
403 and cooking oils.
404 §170.3 (n)(35) Processed fruits and fruit juices, including all commercially processed fruits, citrus,
405 berries, and mixtures; salads, juices and juice punches, concentrates, dilutions, ―ades‖, and drink
406 substitutes made therefrom.‖
407
408 β-Carotene was evaluated by the Select Committee on GRAS Substances (SCOGS) in 1979. The SCOGS
409 concluded that there was no evidence in the available information on β-carotene that demonstrated, or
410 suggested reasonable grounds to suspect a hazard to the public when it was used at levels at that time or
411 might reasonably be expected in the future (SCOGS Report No. 111).
412
413 In addition, β-carotene is listed under Everything Added to Food in the United States (EAFUS) in
414 FDA/CFSAN’s the Priority-based Assessment of Food Additives (PAFA) database. The EAFUS list of
415 substances contains ingredients added directly to food that FDA has either approved as food additives or
416 listed or affirmed as GRAS.
417
418 Evaluation Question #5: Describe whether the primary function/purpose of the petitioned substance is
419 a preservative. If so, provide a detailed description of its mechanism as a preservative. (7 CFR § 205.600
420 (b)(4))
421
422 No information sources reviewed specifically address the primary function/purpose of β-carotene as a
423 preservative.
424
425 Evaluation Question #6: Describe whether the petitioned substance will be used primarily to recreate
426 or improve flavors, colors, textures, or nutritive values lost in processing (except when required by law)
427 and how the substance recreates or improves any of these food/feed characteristics. (7 CFR § 205.600
428 (b)(4))
429
430 The petitioned substance is under FDA Regulation PART 73 ― LISTING OF COLOR ADDITIVES EXEMPT
431 CERTIFICATION. The color additive is β-carotene prepared synthetically or obtained from natural sources
432 (21 CFR §73.95(a)(1)); it may be safely used for coloring foods generally, in amounts consistent with good
433 manufacturing practice, except that it may not be used to color those foods for which standards of identity
434 have been promulgated unless added color is authorized by such standards (21 CFR §73.95(c)). According
435 to FDA the standard of identity for margarine, it stipulates ―…provitamin A (β-carotene) shall be deemed
436 to be a color additive‖ (21 CFR §166.110(b)(6)). β-Carotene imparts a yellow color to foods.
437
438 When β-Carotene is used as food colorant, its concentrations generally are between 2 and 50 parts per
439 million (ppm) so that its color contribution to the foods is from yellow to orange (Ribeiro and others, 2011).
440 As Dziezak (1987) notes, colorants are added to consumable products for the sole purpose of enhancing the
441 visual appeal. The reasons for adding colors to foods include (Aberoumand, 2011):
442 to replace color lost during processing,
443 to enhance color already present,
444 to minimize batch to batch variations, and
445 to color otherwise uncolored food.
446
447 β-Carotene can also be used as a nutrient ingredient to replace vitamin A lost in processing, or as an added
448 nutrient that may be lacking in the diet (FDA Website, Types of Food Ingredients, 2010). It may be added
449 in flour, breads, cereals, rice, macaroni, margarine, salt, milk, fruit beverages, energy bars, and instant
450 breakfast drinks.
451
452 Evaluation Question #7: Describe any effect or potential effect on the nutritional quality of the food or
453 feed when the petitioned substance is used. (7 CFR § 205.600 (b)(3))
454
455 The petitioned substance is a precursor of vitamin A (also called a provitamin A carotenoid). According to
456 the FDA regulations, the affirmation of β-carotene as GRAS is used as a nutrient supplement
457 (§184.1245(c)(1)) and it may be used in infant formula as a source of vitamin A (§184.1245(c)(2)). Vitamin A
458 is a fat-soluble vitamin that is essential for humans and other vertebrates. It is important for normal vision,
459 gene expression, reproduction, embryonic development, growth, and immune function.
460
461 In the report on Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine,
462 Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc by Food and Nutrition Board of Institute of
463 Medicine (IOM) (2001), it has indicated that current dietary patterns appear to provide sufficient vitamin A
464 to prevent deficiency symptoms such as night blindness. The estimated average requirement is based on
465 the assurance of adequate stores of vitamin A. The Recommended Dietary Allowance (RDA) for men and
466 women is 900 and 700 μg retinol activity equivalents (RAE)/day or 3000 and 2310 International Units
467 (IU)/day, respectively (IOM, 2001). [Note: 1 RAE = 3.3 IU] However, there is no RDA for β-carotene. The
468 IOM (2001) stated that consuming 3 mg to 6 mg of β-carotene daily (equivalent to 833 IU to 1,667 IU
469 vitamin A) will maintain blood levels of β-carotene in the range associated with a lower risk of chronic
470 diseases.
471
472 At present, it is unclear whether the biological effects of carotenoids in humans are a result of their
473 antioxidant activity or other non-antioxidant mechanisms (LPI, 2009). Some provitamin A carotenoids
474 have been shown to function as antioxidants in laboratory studies; however, this role has not been
475 consistently demonstrated in humans (IOM, 2001). Although, the FDA’s food labeling regulation (21 CFR
476 §101.54(g)(3)) indicates that β-carotene may be a subject of the claim when the level of vitamin A present as
477 β-carotene in the food that bears the claim is sufficient to qualify for the claim. For example, for the claim
478 ―good source of antioxidant β-carotene,‖ 10 percent or more of the Recommended Daily Intake for vitamin
479 A must be present as β-carotene per reference amount customarily consumed.
480
481 Evaluation Question #8: List any reported residues of heavy metals or other contaminants in excess of
482 FDA tolerances that are present or have been reported in the petitioned substance. (7 CFR § 205.600
483 (b)(5))
484
485 According to the specification of β-carotene in Food Chemical Codex (2010-2011), it stipulates the impurity
486 acceptable criterion for a heavy metal is not more than 5 mg/kg (5 ppm) lead. Moreover, the specification
487 of the color additive β-carotene, which may be safely used for coloring foods, in FDA regulation (21 CFR
488 §73.95(b)) specifies that lead is not more the 10 ppm and arsenic is not more than 3 ppm.
489
490 No information sources can be identified to suggest that the petitioned substance contains residues of
491 heavy metals or other contaminants in excess of FDA’s Action Levels for Poisonous or Deleterious
492 Substances in Human Food.
493
494 Evaluation Question #9: Discuss and summarize findings on whether the manufacture and use of the
495 petitioned substance may be harmful to the environment. (7 U.S.C. § 6517 (c) (1) (A) (i) and 7 U.S.C. §
496 6517 (c) (2) (A) (i))
497
498 I. MANUFACTURE
499
500 The petitioned substance can be produced from synthetic chemicals or natural sources (such as fungi,
501 algae, or plants):
502
503 (A) Synthetic chemicals
504
505 There are two commonly used methods (Grignard and Wittig reactions) of chemical synthesis of β-
506 carotene, see EQ #1. The synthesis process from the Grignard reaction presents a yield of 60%, while the
507 process used the Wittig reaction presents a yield of 85 %. Although the yield of the Wittig reaction method
508 is higher than the Grignard reaction method, the Wittig reaction method has a drawback―low
509 biodegradability of triphenylphosphine oxide, which is used as a catalyst during one chemical reaction
510 step. According to Fisher’s Material Safety Data Sheet (MSDS) (2008), triphenylphosphine oxide is harmful
511 to aquatic organisms and may cause long-term adverse effects in the aquatic environment. This chemical
512 has to be recycled. The industrial recovery process comprises three phases: distillation, chlorination with
513 phosgene, and dehalogenation with aluminum (Ribeiro and others, 2011).
514
515 (B) Natural sources ― the production of β-carotenes are made from renewable sources
516
517 β-Carotene made from filamentous fungi (Blakeslea trispora)
518
519 The fungus Blakeslea trispora lives in commensalism with tropical plants; some strains in nature are big
520 producers of β-carotene and other carotenoids. It has been shown to be nonpathogenic and
521 nontoxigenic (Dufosse, 2006). The fungi are grown in large-scale fermenters using food-grade raw
522 materials, such as glucose, corn steep liquor, and cheese whey (Dufosse, 2006). As in the recovery
523 process, β-carotene is obtained from the fungal biomass by solvent extraction and crystallized with
524 high purity, see EQ #2. Ishida and Chapman (2009) reported that ethyl acetate is most commonly used
525 for extracting carotenoids. Ethyl acetate is not considered to be environmentally friendly and is highly
526 flammable (explosive). Although it can be produced by reaction of ethanol and acetic acid, its primary
527 source is from petroleum (Ishida and Chapman, 2009).
528
529 β-Carotene made from microalgae (Dunaliella salina)
530
531 Dunaliella species are commonly observed in salt lakes in all parts of the world from tropical to
532 temperate to polar regions where they often impart an orange-red color to the water. As in commercial
533 cultivation of the production, β-carotene is accumulated as droplets in the algal chloroplast stroma,
534 especially under the environmental conditions in high temperature, high salinity, high irradiance, and
535 nutrient limitation (low nitrogen). Then, β-carotene may be obtained from algal biomass or dried
536 powder by using hot edible oil extraction and supercritical carbon dioxide, see EQ #2.
537
538 In addition, it is desirable to re-utilize the culture medium remains after harvesting (biomass removal).
539 Dunaliella growth medium could be recycled biologically by treating the medium with bacteria that are
540 naturally present in medium because of the high concentration of glycerol, amino acids, and other
541 organic compounds (Ben-Amotz, 1995). In a review article conducted by Dufosse et al. (2005), they
542 concluded that algal forms are the richest source of pigments and can be produced in a renewable
543 manner, since they produce some unique pigments sustainably. The report also stated that the
544 production of β-carotene from Dunaliella will surpass synthetic as well as other natural sources due to
545 microalgae sustainability of production and their renewable nature.
546
547 β-Carotene made from plant extraction
548
549 β-Carotene is extracted from plant material using a solvent, such as hexane, acetone, ethyl acetate,
550 ethanol, and ethyl lactate. Among these solvents, ethyl lactate is an environmentally friendly solvent
551 produced from the fermentation of carbohydrate feedstock available from the corn and soybean
552 industries (Ishida and Chapman, 2009). Colorless ethyl lactate has a relatively high flashpoint, is
553 environmentally benign, and can be completely biodegraded into CO2 and water. In Ishida and
554 Chapman’s research (2009), they indicated that ethyl lactate is almost as efficient as ethyl acetate, which
555 is most commonly used for extracting carotenoids to be used in food products, for the extraction of β-
556 carotene.
557
558 II. USE
559
560 No Occupation Safety and Health Administration (OSHA) Vacated Permissible Exposure Limits (PELs) are
561 listed for β-carotene.
562
563 According the MSDS in the petition, it stated ―Natural Carotene WD 20 AP is the extract of natural
564 carotenoids; rendered water soluble using a blend of maltodextrin modified starch, sugar and MCT oil. Dl-
565 α-tocopherol & ascorbic acid are added as anti-oxidants.‖ It also showed that ingredient is β-carotene and
566 its CAS No. is 33261-80-20. [Note: 33261-80-20 is NOT the CAS No. for β-carotene. Moreover, the content
567 of β-carotene is not specified.] For ecological information, it stated ―Natural Carotene WD 20 AP is
568 biodegradable. Do not allow to enter natural waterways.‖ This product should be handled in accordance
569 with good occupational hygiene and safety practices, and avoid contact with skin and eyes. The workers
570 should wear appropriate protective eyeglasses (or chemical safety goggles), gloves, and clothing; in
571 addition to use suitable dust mask or breathing apparatus where aerosols created.
572
573 Evaluation Question #10: Describe and summarize any reported effects upon human health from use of
574 the petitioned substance. (7 U.S.C. § 6517 (c) (1) (A) (i), 7 U.S.C. § 6517 (c) (2) (A) (i) and 7 U.S.C. § 6518
575 (m) (4))
576
577 As stated above, see EQ # 7, β-carotene is a vitamin A precursor or a provitamin A carotenoid. Absorbed
578 β-carotene can be converted by the body to retinol and be subsequently made into retinal and retinoic acid
579 (other forms of vitamin A) (IOM, 2001). Vitamin A is used by eyes to synthesize the light-sensitive retinal
580 pigments. In addition, vitamin A plays an important role in bone growth, reproduction, cell division, and
581 cell differentiation (in which a cell becomes part of the brain, muscle, lungs, blood, or other specialized
582 tissue.); it helps regulate the immune system, which will prevent or fight off infections by making white
583 blood cells that destroy harmful bacteria and viruses (IOM, 2001). Vitamin A also may help lymphocytes
584 (a type of white blood cell) fight infections more effectively. In addition, vitamin A promotes healthy
585 surface linings of the eyes and the respiratory, urinary, and intestinal tracts (Semba, 1998). When those
586 linings break down, it becomes easier for bacteria to enter the body and cause infection. It also helps the
587 skin and mucous membranes function as a barrier to bacteria and viruses (Ross, 1999; Harbige, 1996).
588
589 According to IOM’s report released in 2001, it has indicated that although a large body of observational
590 epidemiological evidence suggests that higher blood concentrations of β-carotenes and other carotenoids
591 obtained from foods are associated with a lower risk of several chronic diseases, there is currently not
592 sufficient evidence to support a recommendation that requires a certain percentage of dietary vitamin A to
593 come from provitamin A carotenoids in meeting the vitamin A requirement. However, IOM Dietary
594 Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids of Year 2000 recommended the
595 increase of consumption of carotenoid-rich fruits and vegetables for their health-promoting benefits. In
596 addition, the IOM 2001 report stated that "β-carotene supplements are not advisable for the general
597 population," although they also state that this advice "does not pertain to the possible use of supplemental
598 β-carotene as a provitamin A source for the prevention of vitamin A deficiency in populations with
599 inadequate vitamin A".
600
601 Provitamin A carotenoids such as β-carotene are generally considered safe because they are not associated
602 with specific adverse health effects (NIH, 2006). Their conversion to vitamin A decreases when body stores
603 are full. A high intake of provitamin A carotenoids can turn the skin yellow, but this is not considered
604 dangerous to health. According to the Select Committee on GRAS Substances (SCOGS) Report on
605 ―Carotene (β-carotene)‖ (1979), it concluded that ―There is no evidence in the available information on
606 carotene (β-carotene) that demonstrates, or suggests reasonable grounds to suspect, a hazard to the public
607 when it is used at levels that are now current or that might reasonably be expected in the future.‖
608
609 The Joint FAO/WHO7 Expert Committee on Food Additives (JECFA) has evaluated several β-carotenes
610 (which may be produced by chemical synthesis or obtained by extraction from a microorganism, algae, or
611 vegetables) containing the same chemical entity as the functional component in relation to its food additive
612 use but obtained from different source materials and/or different manufacturing processes. The
613 Committee has reached various conclusions in its evaluations for an acceptable daily intake (ADI) for a
614 man:
615
616 (A) A group ADI of 0-5 mg/kg body weight (bw) for β-carotene, synthetic and from Blakeslea trispora,
617 established at the 57th JECFA in 2001. [Note: 0-5 mg as sum of the carotenoids including β-carotene, β-apo-
618 8’-carotenal, β-carotenoic acid methyl ester, and β-carotenoic acid ethyl ester (WHO FAS 6, 1975).]
619
620 In the 57th report of JECFA on Safety Evaluation of Certain Food Additives and Contaminants― β-
621 carotene derived from blakeslea trispora (WHO FAS 48, 2001), the Committee concluded that, on the
622 basis of the source organisms, the production process, and its composition characteristics, β-carotene
623 from B. trispora does not raise specific concerns and from a toxicological point of view should be
624 considered equivalent to chemically synthesized β-carotene, for which an ADI of 0–5 mg/kg bw was
625 established by the Committee at its 18th meeting (see below). This opinion was supported by the
626 negative results in two tests for genotoxicity (mutagenesis and chromosomal aberration) considered at
627 the 57th meeting. Therefore, the Committee established a group ADI of 0–5 mg/kg bw for synthetic β-
628 carotene and β-carotene derived from B. trispora. This ADI applies to use of β-carotene as a coloring
629 agent and not to its use as a food supplement (WHO FAS 48, 2001).
630
631 A β-Carotene toxicological evaluation was conducted at the 18th meeting of JECFA in 1974. In the
632 report published the next year (WHO FAS 6, 1975), the Committee stated that β-carotene is a normal
633 constituent of the human diet and is commonly ingested over the entire lifespan of man. Its biological
634 importance rests on the provitamin A function. Concerning the known clinical syndrome of
635 hypervitaminosis A in man, evidence from human experience indicates that in very exceptional
636 circumstances excessive dietary intakes can occur. Such cases have been reported in the literature but
637 do not relate to food additive use of this color. Despite poor absorption from the gastrointestinal tract
638 cases of human hypervitaminosis have occurred. The results of short-term toxicity studies in rats and
639 dogs have shown that over a wide range of doses toxic effects have not been produced. Similarly,
640 multi-generation tests in rats using levels up to 1000 ppm have not revealed any adverse effects. In
641 addition, the JECFA concluded that ―In the light of the above comments it appears justifiable to apply a
642 smaller safety factor to the no-effect level established in long-term studies.‖ Furthermore, estimate of
643 ADI, 0-5 mg/kg bw, was established at the 18th meeting (WHO FAS 6, 1975).
644
645 According to WHO Technical Report Series No. 557 (1974), it indicated that carotenes (natural) were
646 reviewed at the 18th meeting by the Committee when it was concluded that further information was
647 required before a specification could be developed. Therefore, no toxicological evaluation was
648 prepared and no ADI was established for natural carotene at that time. ADI of 0-5 mg/kg bw was
649 established for synthetic carotene.
650
651 (B) No ADI allocated for β-carotene from algae established at the 41st JECFA in 1993.
652
653 Carotenes from natural sources (algal and vegetable) are reviewed by the JECFA at the 41st meeting
654 and reported on Toxicological Evaluation of Certain Food Additives and Contaminants of WHO Food
655 Additives Series No. 32. The Committee considered the data inadequate to establish an ADI for the
656 dehydrated algal carotene preparations or for the vegetable oil extracts of Dunaliella salina. [Note:
657 There is no history of use of Dunaliella algae as food (WHO FAS 32, 1993).]
658
659 (C) ADI ―acceptable‖ for β-carotene from vegetables, provided the level of use does not exceed the level
660 normally found in vegetables, established at the 41st JECFA in 1993.
661
662 In the toxicological monograph (WHO FAS 32, 1993), the JECFA identified that no relevant
663 toxicological data on vegetable extracts were available. However, the Committee concluded that there
664 was no objection to the use of vegetable extracts as coloring agents, provided that the level of use did
665 not exceed the level normally present in vegetables. The report stated that ―implicit in this conclusion
666 is that the extracts should not be made toxic by virtue of the concentration of toxic compounds
667 (including toxicants naturally occurring in the vegetables) nor by the generation of reaction products or
668 residues of a nature or in such amounts as to be toxicologically significant.‖
669
670 Evaluation Question #11: Provide a list of organic agricultural products that could be substituted for
671 the petitioned substance. (7 CFR § 205.600 (b)(1))
672
673 Currently, ―beta-carotene extract color, derived from carrots (CAS # 1393–63–1)‖ is listed on NOP the
674 National List of Allowed and Prohibited Substance under § 205.606 Nonorganically produced agricultural
675 products allowed as ingredients in or on processed products labeled as ―organic.‖ (d) Colors derived from
676 agricultural products (7 CFR §205.606 (d)(3)).
677
678 Organic annatto extract is an organically produced agricultural ingredient that could be substituted for the
679 petitioned substance. According to 606organic.com, a website administered and maintained by the
680 Accredited Certifiers Association, Inc., annatto extract color is commercially available in an organic form
681 from D. D. Williamson & Co., Inc. [Note: D. D. Williamson & Co., Inc. is also the petitioner for this
682 substance (beta-carotene extract color).]
683
684 In the FDA regulations, annatto extract is a food color additive and is exempted from certification listed in
685 21 CFR §72.30. Annatto extract may be safely used for coloring foods generally, in amounts consistent with
686 good manufacturing practice, except that it may not be used to color those foods for which standards of
687 identity have been promulgated unless added color is authorized by such standards (21 CFR §73.30(c)).
688 Certification of this color additive is not necessary for the protection of the public health in accordance with
689 21 CFR §72.30 (e). The yellow to orange colors of annatto comes from the outer layer of seeds of the
690 tropical tree Bixa orellana. The carotenoids (bixin and norbixin) are responsible for the appearance of the
691 yellow to orange colors. The pH and solubility affect the color hue; the greater the solubility in oil, the
692 brighter is the color. Annatto extract are available in water soluble, oil soluble, and oil/water dispersible
693 forms. Since it precipitates at low pH, it is also available as an emulsion, an acid proof state. Annatto has
694 been used for over two centuries as a food color especially in cheese and in various other food products
695 (Gordon and others, 1982; Aberoumand, 2011).
696
697 Based on the database of NOP Certified Operations, as of 2010, following is a tabulated list for the names
698 and addresses of companies producing or handling organic annatto (NOP Certified Operations, 2010):
699
COMPANY ADDRESS
Fundación Chankuap Vidal Rivadeneira y Hernando de Benavente, Macas,
Morona Santiago, EC Ecuador
Productos SKS Farms Cía. Ltda. Julio Zaldumbide 398 y Mira Valle, Quito, Pichincha, EC
Ecuador
Whole Herb Co. Sonoma, CA 95476
Fores Trade Europe Wijnkoopsbaai 16 Capelle a/d Ijssel, 2904 BP, Netherland
Aryan International FZC P.O. Box.- 5232, Fujairah, United Arab Emirates
PR 200 - APROAP Caixa Postal 149, Umuarama - PR CEP: 87502-970, Brazil
BA 036 - Coop. dos Produtores Org. do Rua Jasmim, Nº 25 Nelson Costa, Ilhéus - BA CEP: 45656-
Sul da Bahia - CABRUCA 140, Brazil
Superior Natural Foods 44 St. Croix Trail South, Lakeland, MN 55043
700
701
702 References
703
704 Aberoumand, A. 2011. A Review Article on Edible Pigments Properties and Sources as Natural
705 Biocolorants in Foodstuff and Food Industry. World Journal of Dairy & Food Sciences 6 (1): 71-78, 2011.
706
707 Accredited Certifiers Association, Inc., 606organic website. Organic Β-carotene extract color. In:
708 606organic.com. [Accessed April 29, 2011] http://606organic.com/results.php?product=Β-
709 carotene%20extract%20color
710
711 Ball, G. 1996. Determination of the fat-soluble vitamins in foods by high-performance liquid
712 chromatography. In: Handbook of Food analysis. Vol. 1. Physical Characterization and Nutrient Analysis.
713 Marcel Dekker, New York, p 602-604.
714
715 BCC Research. 2008. Global Market for Carotenoids. Food and Beverage. March 2008.
716 http://www.bccresearch.com/report/carotenoids-market-fod025c.html
717
718 Ben-Amotz, A. 1995. New mode of Dunaliella Biotechnology: two-phase growth for β-carotene production.
719 J Appl Phycol 7, 65-68.
720
721 Ben-Amotz, A., Gressel, J., Avron, M. 1987. Massive Accumulation of phytoene induced by norflurazon in
722 Dunaliella bardawil (Chlorophyceae) prevents recovery from photoinhibition. J Phycol 23:176–181
723
724 Britton, G., Liaaen-Jensen, S., and Pfander, H. 1996. Carotenoids. (Vol. 2: Synthesis). Stuttgart, Germany:
725 Birkhaüser Verlag Basel.
726
727 β-CAROTENE, synthetic. 2006. In: JECFA Specification Monograph. http://www.fao.org/ag/agn/jecfa-
728 additives/specs/Monograph1/additive-113-m1.pdf
729
730 β-CAROTENE from BLAKESLEA TRISPORA. 2007. In: JECFA Specification Monograph.
731 http://www.fao.org/ag/agn/jecfa-additives/specs/monograph4/additive-112-m4.pdf
732
733 β-Carotene. 2011. In: Schedule IV, Part II, Class 7. Vitamin Products and Yeast Products. Proposed
734 amendments to the Feeds Regulations. Canadian Food Inspection Agency. 2011-03-25.
735 http://www.inspection.gc.ca/english/anima/feebet/ind/sched/schedu4je.shtml
736
737 β-CAROTENE (Vegetable). 2006. In: JECFA Specification Monograph. http://www.fao.org/ag/agn/jecfa-
738 additives/specs/Monograph1/additive-115-m1.pdf
739
740 Carotene. 2006. In: Food Additives Permitted for Use in Canada―Health Canada. 2006-12-11.
741 http://www.hc-sc.gc.ca/fn-an/securit/addit/diction/dict_food-alim_add-eng.php#c
742
743 Carotene. 2007. In: Natural Health Products Ingredients Database ―Health Canada. 2007-04-18.
744 http://webprod.hc-sc.gc.ca/nhpid-bdipsn/ingredReq.do?id=1048&lang=eng
745
746 CAROTENES (Algae). 2007. In: JECFA Specification Monograph. http://www.fao.org/ag/agn/jecfa-
747 additives/specs/monograph4/additive-114-m4.pdf
748
749 Carotene, β- (368. Carotene, β-). 1975. In: WHO Food Additive Series 6. Toxicological Evaluation of Some
750 Food Colours, Enzymes, Flavour Enhancers, Thickening Agent, and Certain Food Additives. World Health
751 Organization, Geneva, 1975.
752
753 Carotenes, Β-(Vegetable); Carotenoids. 2010. In: Codex General Standard for Food Additives, Table
754 One―Additives Permitted for Use Specified Conditions in Certain Food Categories or Individual Food
755 Items. CODEX STAN 192-1995. Revision 2010. p 66-69.
756
757 Davies, H. 1976. In: Goodwin, T.W. ed. Chemistry and Biochemistry of Plant Pigments. London:
758 Academic Press, 1976, p 38.
759
760 Directive 94/36/EC. In: Official Journal of the European Communities. No L 237. 10.9.94. European
761 Parliament and Council Directive 94/36/EC of 30 June 1994 on colours for use in food stuffs. p 13-18.
762
763 Directive 2004/47/EC. In: Official Journal of the European Union. No L 113. 20.4.2004. Commission
764 Directive 2004/47/EC of 16 April 2004 amending Directive 94/45/EC as regards mix carotenes (E 160a(i))
765 and β-carotene (E 160a(ii)). p 24-27.
766
767 Dufosse, L., Galaup, P., Yaron, A., Arad, S.M., Blanc, P., Murthy, K., and Ravishankar, G. 2005.
768 Microorganisms and microalgae as sources of pigments for food use: a scientific oddity or an industrial
769 reality? Trends in Food Science & Technology 16 (2005) 389-406.
770
771 Dufosse, L. 2006. Microbial Production of Food Grade Pigments. Food Technol. Biotechnol. 44(3) 313-321.
772
773 Dziezak, J.D., 1987. Applications of food colorants. Food Technol. 41 (4):78-88.
774
775 Echavarri-Erasun, C. and Johnson, E.A. 2002. Fungal Carotenoids. In: Applied Mycology and
776 Biotechnology. Vol. 2. Agriculture and Food Production. Elsevier Science B.V.
777
778 EPA, List 4A-Minimal Risk Inert Ingredients – By Chemical Name. In: List of Inert Pesticide Ingredients.
779 Updated August 2004. EPA, Office of Pesticide Programs.
780
781 EPA, List 4B-Other Ingredients for which EPA has sufficient information to reasonably conclude that the
782 current use pattern in pesticide products will not adversely affect public health or the environment – By
783 Chemical Name. In: List of Inert Pesticide Ingredients. Updated August 2004. EPA, Office of Pesticide
784 Programs.
785
786 EPA, Substance Registry Services. 2011.
787 http://iaspub.epa.gov/sor_internet/registry/substreg/searchandretrieve/advancedsearch/search.do?sea
788 rch=?search=&searchCriteria(advancedCriteria)=casNumber=7235-40-7. Last updated on June 16, 2011.
789
790 Food Chemicals Codex (FCC). 2010-2011. β-Carotene. In: Food Chemicals Codex. 7th ed. Rockville, MD:
791 The US Pharmacopeial Convention. p 180.
792
793 FDA, 21 CFR §73.30 Annatto extract. http://ecfr.gpoaccess.gov/cgi/t/text/text-
794 idx?c=ecfr&sid=b6c4a8d11fbd668f0b20d393b2d30208&rgn=div8&view=text&node=21:1.0.1.1.26.1.31.2&idn
795 o=21
796
797 FDA, 21 CFR §73.95 β-Carotene. http://ecfr.gpoaccess.gov/cgi/t/text/text-
798 idx?c=ecfr&sid=0934c97f71569f12caee3d89f4a4e8b5&rgn=div8&view=text&node=21:1.0.1.1.26.1.31.10&idn
799 o=21
800
801 FDA, 21 CFR §73.1095 β-Carotene. http://ecfr.gpoaccess.gov/cgi/t/text/text-
802 idx?c=ecfr&sid=0934c97f71569f12caee3d89f4a4e8b5&rgn=div8&view=text&node=21:1.0.1.1.26.2.31.9&idno
803 =21
804
805 FDA, 21 CFR §73.2095 β-Carotene. http://ecfr.gpoaccess.gov/cgi/t/text/text-
806 idx?c=ecfr&sid=0934c97f71569f12caee3d89f4a4e8b5&rgn=div8&view=text&node=21:1.0.1.1.26.3.31.4&idno
807 =21
808
809 FDA, 21 CFR §101.54(g)(3). In: Nutrient content claims for ―good source,‖ ―high,‖ ―more,‖ and ―high
810 potency.‖ http://ecfr.gpoaccess.gov/cgi/t/text/text-
811 idx?c=ecfr&rgn=div8&view=text&node=21:2.0.1.1.2.4.1.1&idno=21
812
813 FDA, 21 CFR §166.110 Margarine. http://ecfr.gpoaccess.gov/cgi/t/text/text-
814 idx?c=ecfr&sid=0934c97f71569f12caee3d89f4a4e8b5&rgn=div8&view=text&node=21:2.0.1.1.39.2.1.1&idno=
815 21
816
817 FDA, 21 CFR §170.3 Definitions. http://ecfr.gpoaccess.gov/cgi/t/text/text-
818 idx?c=ecfr&sid=455948b57c6c700436a0f490c7d3f56a&rgn=div8&view=text&node=21:3.0.1.1.1.1.1.1&idno=
819 21
820
821 FDA, 21 CFR §184.1245 Β-carotene. http://ecfr.gpoaccess.gov/cgi/t/text/text-
822 idx?c=ecfr&sid=0934c97f71569f12caee3d89f4a4e8b5&rgn=div8&view=text&node=21:3.0.1.1.14.2.1.57&idno
823 =21
824
825 FDA, Β-Carotene, Doc No. 1857. In: Everything Added to Food in the United States (EAFUS).
826 FDA/CFSAN’s the Priority-based Assessment of Food Additives (PAFA) database.
827 http://www.accessdata.fda.gov/scripts/fcn/fcnDetailNavigation.cfm?rpt=eafusListing&id=531
828
829 FDA, SCOGS Report No. 111. 1979. Carotene (β-carotene). In: Database of Select Committee on GRAS
830 Substances (SCOGS) Reviews.
831 http://www.accessdata.fda.gov/scripts/fcn/fcnDetailNavigation.cfm?rpt=scogsListing&id=75
832
833 FDA website, Types of Food Ingredients. 2010. in Food Ingredients and Colors. International Food
834 Information Council (IFIC) and U.S. Food and Drug Administration November 2004; revised April 2010.
835 http://www.fda.gov/food/foodingredientspackaging/ucm094211.htm#types
836
837 Fisher Scientific. 2008. Material Safety Data Sheet (MSDS): Triphenylphosphine oxide. Revised: 2/28/2008.
838 https://fscimage.fishersci.com/msds/85709.htm
839
840 Furia, T.E. 1972. CFC handbook of Food Additives. 2nd edition, Vol.1. CRC Press, Inc. p 190.
841
842 Gordon, H.T., Bauernfeind, J.C., and Furia, T.E. 1982. Carotenoid as food colorants. In: Critical Reviews in
843 Good Science and Nutrition, 18: 1, p. 59-97.
844
845 Halliwell, B. and Gutteridge, J.M.C. 1999. Free Radicals in Biology and Medicine. 3rd ed. New York, NY:
846 Oxford University Press.
847
848 Harbige, L.S. 1996. Nutrition and immunity with emphasis on infection and autoimmune disease. Nutr
849 Health. 10:285-312. [PubMed abstract]
850
851 Institute of Medicine (IOM). 2001. Vitamin A. In: Dietary Reference Intakes for Vitamin A, Vitamin K,
852 Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium,
853 and Zinc. Food and Nutrition Board, Institute of Medicine of the National Academies. The National
854 Academies Press. Washington, D.C. January 9, 2001. p 82.
855
856 Institute of Medicine (IOM). 2000. β-Carotene and Other Carotenoids. In: Dietary Reference Intakes for
857 Vitamin C, Vitamin E, Selenium, and Carotenoids. Food and Nutrition Board, Institute of Medicine of the
858 National Academies. The National Academies Press. Washington, D.C. Vitamin A. August 3, 2000. p 325.
859
860 Ishida, B.K. and Chapman, M.H. 2009. Carotenoid Extraction from Plants Using a Novel, Environmentally
861 Friendly Solvent. Journal of Agricultural and Food Chemistry. 57, p 1051-1059.
862
863 Isler, O. 1971. Carotenoids. Stuttgart, Germany: Birkhaüser Verlag Basel.
864
865 Japan Food Sanitation Law -- Table 1. 2006. Ministry of Health, Labor, and Welfare. Last amendment Law
866 No 87, July 26, 2005. http://www.tokio.polemb.net/files/Gospodarka/Handel/food-e.pdf
867
868 Japan Ministry of Health, Labor, and Welfare: Food Additives -- List of Designated Additives and List of
869 Existing Food Additives. http://www.mhlw.go.jp/english/topics/foodsafety/foodadditives/index.html
870
871 JECFA. 1974. β-Carotene (Synthetic). In: Evaluations of the Joint FAS/WHO Expert Committee on Food
872 Additives (JECFA).
873
874 JECFA. 1989. Carotenes (Natural). In: Evaluations of the Joint FAS/WHO Expert Committee on Food
875 Additives (JECFA).
876
877 JECFA. 1993. Carotenes (Vegetable). In: Evaluations of the Joint FAS/WHO Expert Committee on Food
878 Additives (JECFA).
879
880 JECFA. 1993. Carotenes (Algae). In: Evaluations of the Joint FAS/WHO Expert Committee on Food
881 Additives (JECFA).
882
883 JECFA. 2001. β-Carotene from blakeslea trispora. In: Evaluations of the Joint FAS/WHO Expert Committee
884 on Food Additives (JECFA).
885
886 Krinsky, N.I., Wang, X-D, Tang, G., and Russell, R.M. 1993. Mechanism of carotenoid cleavage to retinoids.
887 Ann NY Acad Sci 691:167–176.
888
889 Linus Pauling Institute (LPI) Micronutrient Information Center. 2009. Carotenoids. In: Phytochemicals.
890 Linus Pauling Institute at Oregon State University.
891
892 Merck Index. 2006. β-Carotene (Monograph No. 1853). In: the Merck Index an Encyclopedia of Chemicals,
893 Drugs, and Biologicals. 14th ed. Whitehouse Station, NJ: Merck & Co., Inc. p 301.
894
895 Mijts, B.N., Lee, P.C., and Schmidt-Dannert, C. 2005. Identification of a carotenoid oxygenase synthesizing
896 acyclic xanthophylls. Combinatorial biosynthesis and directed evolution. Chemistry and Biology. 12, p 453-
897 460.
898
899 National Institutes of Health (NIH). 2006. Dietary Supplement Fact Sheet: Vitamin A and Carotenoids.
900 Office of Dietary Supplements, National Institutes of Health. Updated: 4/23/2006.
901
902 Oren, A. 2010. Industrial and environmental applications of halophile microorganisms. Environmental
903 Technology. Vol. 31, Nos. 8-9, July-August 2010, 825-834.
904
905 Raja, R., Hemaiswarys, S., and Rengasamy, R. 2007. Exploitation of Dunaliella for β-carotene production.
906 Appl Microbiol Biotechnol. 74:517-523.
907
908 Ribeiro, B.D., Barreto, D.W., and Coelho, M.A.Z. 2011. Technological Aspects of β-Carotene Production.
909 Food Bioprocess Technol. DOI 10.1007/s11947-011-0545-3. Published online: 02 March 2011.
910
911 Ross, D.A. 1998. Vitamin A and public health: Challenges for the next decade. Proc Nutr Soc. 57:159-65.
912 [PubMed abstract]
913
914 Russell, A. and Kenyon, R.L. 1943. Organic Syntheses. Vol. 23, p. 78.
915
916 Saari, J.C. 1994. Retinoids in photosensitive systems. In: Sporn MB, Roberts AB, Goodman DS, eds. The
917 Retinoids: Biology, Chemistry, and Medicine, 2nd ed. New York: Raven Press. p 351–385.
918
919 SCF. 2000. Opinion of the Scientific Committee on Food (SCF) on the safety of use of beta carotene from all
920 dietary sources. SCF/CS/ADD/COL/159 Final. Sep. 14, 2000. European Commission Health & Consumer
921 Protection Directorate-General.
922
923 Semba, R.D. 1998. The role of vitamin A and related retinoids in immune function. Nutr Rev. 56:S38-48.
924 [PubMed abstract]
925
926 US Court of International Trade Reports: BASF Corp, Plaintiff, V. The Unite State, Defendant. 2005. Vol.
927 29. p 681-695.
928
929 USDA, NOP Certified Operations. 2010. http://apps.ams.usda.gov/nop/
930
931 Wang, X.D., Krinsky, N., and Benotti, P. 1994. Biosynthesis of 9-cis-retinoic acid from 9-cis-β-carotene in
932 human intestinal mucosa in vitro. Arch Biochem Biophys 313:150–155
933
934 WHO Food Additives Series (FAS) 6. 1975. Β-Carotene. In: Toxicological evaluation of some food colours,
935 enzymes, flavour enhancers, thickening agents, and certain other food additives. WHO Food Additives
936 Series, No. 6, 1975. nos 354-400 on INCHEM.
937 http://www.inchem.org/documents/jecfa/jecmono/v06je15.htm
938
939 WHO Food Additives Series (FAS) 32. 1993. Carotenes From Natural Sources (Algal and Vegetable). In:
940 Toxicological evaluation of certain food additives and contaminants. WHO Food Additives Series, No. 32,
941 1993. nos 783-803 on INCHEM. http://www.inchem.org/documents/jecfa/jecmono/v32je07.htm
942
943 WHO Food Additives Series (FAS) 48. 2001. Food Colour: Β-Carotene Derived From Blakeslea trispora. In:
944 Safety evaluation of certain food additives and contaminants. WHO Food Additives Series, No. 48, 2001.
945 nos 1021-1044 on INCHEM. http://www.inchem.org/documents/jecfa/jecmono/v48je04.htm
946
947 WHO Technical Report Series (TRS) 557. 1974. Evaluation of certain food additives (Eighteenth report of
948 the Joint FAO/WHO Expert Committee on Food Additives). FAO Nutrition Meetings Series, No. 54, 1974;
949 WHO Technical Report Series, No. 557, 1974, and corrigendum.
950