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    Anti-ESAT6 Antibody (11G4) Ab26246: 2 Abreviews 9 References 1 Image

    Uploaded by

    Nabilah
    This document provides product details for an anti-ESAT6 antibody. The antibody is a mouse monoclonal that reacts with Mycobacterium tuberculosis and other species. It binds to an epitope on the ESAT6 antigen between residues 3-15. Applications for the antibody include western blot and ELISA. ESAT6 is a secreted antigen involved in the early immune response to tuberculosis.

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    Anti-ESAT6 Antibody (11G4) Ab26246: 2 Abreviews 9 References 1 Image

    Uploaded by

    Nabilah
    34 views2 pages
    This document provides product details for an anti-ESAT6 antibody. The antibody is a mouse monoclonal that reacts with Mycobacterium tuberculosis and other species. It binds to an epitope on the ESAT6 antigen between residues 3-15. Applications for the antibody include western blot and ELISA. ESAT6 is a secreted antigen involved in the early immune response to tuberculosis.

    Original Description:

    Anti-ESAT6 antibody

    Original Title

    Anti ESAT6 Antibody

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    This document provides product details for an anti-ESAT6 antibody. The antibody is a mouse monoclonal that reacts with Mycobacterium tuberculosis and other species. It binds to an epitope on the ESAT6 antigen between residues 3-15. Applications for the antibody include western blot and ELISA. ESAT6 is a secreted antigen involved in the early immune response to tuberculosis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    34 views2 pages

    Anti-ESAT6 Antibody (11G4) Ab26246: 2 Abreviews 9 References 1 Image

    Uploaded by

    Nabilah
    This document provides product details for an anti-ESAT6 antibody. The antibody is a mouse monoclonal that reacts with Mycobacterium tuberculosis and other species. It binds to an epitope on the ESAT6 antigen between residues 3-15. Applications for the antibody include western blot and ELISA. ESAT6 is a secreted antigen involved in the early immune response to tuberculosis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Product Datasheet

    Anti-ESAT6 antibody [11G4] ab26246


    2 Abreviews 9 References 1 Image

    Overview

    Product name Anti-ESAT6 antibody [11G4]


    Description Mouse monoclonal [11G4] to ESAT6
    Specificity This antibody reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.
    Tested applications WB, ELISA
    Species reactivity Reacts with Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium szulgai and Mycobacterium
    marinum.
    Immunogen Tissue/ cell preparation: PPD from Mycobacterium tuberculosis (Tuberculin PPD).

    Epitope This antibody binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 of ESAT6.

    Properties

    Form Liquid
    Storage instructions Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    Storage buffer Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
    Purification notes Protein A/G purified.
    Clonality Monoclonal
    Clone number 11G4
    Myeloma x63-Ag8.653
    Isotype IgG1
    Light chain type kappa

    Applications

    Our Abpromise guarantee covers the use of ab26246 in the following tested applications.
    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
    Application Abreviews Notes
    WB Use at an assay dependent concentration. Detects a band of approximately 10 kDa.

    Detects a band of approximately 10 kDa in short term culture of M. tuberculosis.

    ELISA 1/3200.

    Target

    Relevance The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early
    phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG.
    Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a
    protective immune response.
    Cellular localization Secreted

    Anti-ESAT6 antibody [11G4] images

    http://www.abcam.com/ESAT6-antibody-11G4-ab26246.html 1
    Product Datasheet
    All lanes : Anti-ESAT6 antibody [11G4] (ab26246) at
    1/5000 dilution

    Lane 1 : M. tuberculosis culture filtrate


    Lane 2 : M. tuberculosis culture filtrate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse monoclonal at
    1/100000 dilution
    developed using the ECL technique
    Western blot - Anti-ESAT6 antibody [HYB 076-08]
    (ab26246) Observed band size : 11 kDa

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Please note: All products are "FOR RESEARCH USE ONLYAND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Our Abpromise to you: Quality guaranteed and expert technical support

    Replacement or refund for products not performing as stated on the datasheet


    Valid for 12 months from date of delivery**
    Response to your inquiry within 24 hours

    We provide support in Chinese, English, French, German, Japanese and Spanish


    Extensive multi-media technical resources to help you
    We investigate all quality concerns to ensure our products perform to the highest standards

    If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise
    or contact our technical team.

    Terms and conditions

    Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
    **Regional variations to our Abpromise may apply to the following countries: China, Korea, Singapore, Malaysia, Taiwan and Thailand, which operate a 120 day guarantee.
    Please contact your regional office for further details

    Visit us at: www.abcam.com

    http://www.abcam.com/ESAT6-antibody-11G4-ab26246.html 2

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