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Food Safety and Quality Reference Guide
Food Safety and Quality Reference Guide
This booklet is provided by Bio-Rad only for the use of Bio-Rad products. It is not a contractual document.
QUALITY INDICATORS
PCA
Ordering Information
ISO 6887-1:1999 ISO 4833:2003
Preparation of test samples, General guidance for the Catalog # Description
initial suspension, and tenfold enumeration of microorganisms
Buffered Peptone Water Broth
dilutions for microbiological Ready to use
Colony-count technique at 30°C
examination 3554179 225 ml x 6 bottles
3555795 3 L x 4 bags
Part 1: General rules for
3555790 5 L x 2 bags
preparation of dilutions for
microbiological examination Dehydrated
3564684 500 g
ISO Standards
ISO 16649-1:2001 ISO 16649-2:2001
Horizontal method for the enumeration of Horizontal method for the enumeration of
b-glucuronidase–positive E. coli b-glucuronidase–positive E. coli
Part 1: Colony-count technique at 44°C using Part 2: Colony-count technique at 44°C using
membranes and 5-bromo-4-chloro-3-indolyl 5-bromo-4-chloro-3-indolyl b-D-glucuronide
b-D-glucuronide
Dilutions Dilutions
44°C
18–24 hr
44°C
18–24 hr
Direct enumeration
ISO Standards
ISO/TS 16649-3:2005 ISO 7251:2005
Horizontal method for the enumeration of General guidance for detection
β-glucuronidase–positive E. coli and enumeration of presumptive E. coli
Part 3: Most probable number technique using Most probable number technique
5-bromo-4-chloro-3-indolyl b-D-glucuronide
37°C
Single or* Double
24 ± 2 hr concentration concentration
37°C
24–48 ± 2 hr
Subculture each tube presenting
yellow coloration on a dish of TBX EC Medium
44°C 44°C
20–24 hr 24–48 ± 2 hr
Peptone Water
44°C
48 ± 2 hr
Each tube showing blue colonies on TBX
should be considered positive Indole test reading and calculation
Dehydrated
3564684 500 g
Sample/initial suspension EC Medium Broth
Dehydrated
3564764 500 g
Dilutions
Kovacs Reagent
(for Indole Detection)
3555313 15 ml x 2 bottles
Dehydrated
3564024 500 g
TBX Agar
Ready to use
3555309 100 ml x 6 bottles
Dehydrated
3564035 500 g
Dehydrated
3564544 500 g
* Enumeration of typical colonies for E. coli and coliforms at 37°C; E. coli at 44°C.
Coliforms
Coliforms
VRBL
ISO Standards
ISO 4831:2006 ISO 4832:2006 )
Horizontal method for the detection and enumeration of coliforms Horizontal method for the
Microbiology of food and animal feeding stuffs enumeration of coliforms
Microbiology of food and
MPN technique animal feeding stuffs
Colony-count technique
Dilutions Dilutions
VRBL
Single concentration or* Double concentration
30–37°C
24 ± 2 hr
30 or 37°C 30 or 37°C
24–48 ± 2 hr 24 ± 2 hr
Enumeration of characteristic
BGBLB colonies and confirmation
for atypical colonies
30 or 37°C
24 hr (+24 hr) Coliforms
– Diameter ≥0.5 mm
– Purple with or without
a red zone of
precipitated bile
Reading (gas +)
Dilutions
Dehydrated
3564684 500 g
RAPID'E.coli 2 Agar
Ready to use
3555297 200 ml x 6 bottles
3555299 100 ml x 6 bottles
Dehydrated
RAPID'E.coli 2 3564024 500 g
Dehydrated
3564544 500 g
Dehydrated
3564594 500 g
* Enumeration of typical colonies for E. coli at 44°C; for E. coli and coliforms at 37°C.
Enterobacteriaceae
Enterobacteriaceae
VRBG
ISO Standards
Ordering Information
ISO 21528-1:2004 ISO 21528-2:2004 Catalog # Description
Horizontal methods for the Horizontal methods for the
Buffered Peptone Water Broth
detection and enumeration of detection and enumeration of
Ready to use
Enterobacteriaceae Enterobacteriaceae
3554179 225 ml x 6 bottles
Microbiology of food and animal Microbiology of food and animal 3555795 3 L x 4 bags
feeding stuffs feeding stuffs 3555790 5 L x 2 bags
Part 1: Detection and Part 2: Colony-count technique
Dehydrated
enumeration by MPN technique
3564684 500 g
with pre-enrichment
EE Broth
Dehydrated
3564794 500 g
Sample Sample/initial suspension
Nutrient (2.8%) Agar
Nonselective Ready to use
pre-enrichment Dilutions
3554478 8 ml x 25 tubes
Buffered Peptone Water Dehydrated
37°C 3564484 500 g
18 ± 2 hr 2x*
Violet Red Bile Glucose (VRBG) Agar
Ready to use
VRBG 3554239 100 ml x 6 bottles
30 or 37°C Dehydrated
24 ± 2 hr 3564584 500 g
VRBG Reading
30 or 37°C
24 ± 2 hr
Confirmation Confirmation
Nonselective Agar Nonselective Agar
37°C 37°C
24 ± 2 hr 24 ± 2 hr
RAPID'Enterobacteriaceae
Alternative Method
Ordering Information
RAPID'Enterobacteriaceae Catalog # Description
Enumeration of Enterobacteriaceae
RAPID'Enterobacteriaceae Agar
Ready to use
3554012 200 ml x 6 bottles
Dehydrated
3564004 500 g
RAPID'Enterobacteriaceae
37* ± 1°C
24 ± 2 hr
Direct enumeration**
Salmonella
ISO Standard
ISO 6579:-1:2017
Microbiology of the food chain —
Horizontal method for the detection, enumeration, and serotyping of Salmonella
Part 1: Detection of Salmonella spp.
Sample
Plating out
34−38°C
24 ± 3 hr
Biochemical and Biochemical and
serological* confirmation serological* confirmation
Sample Sample
Non- Non-
selective selective Depending 37°C
pre- pre- on the food 18–24 hr
enrichment enrichment
Bismuth Sulfite
Confirmation
Salmonella Other
spp. Enterobacteriaceae
RAPID'Salmonella
Alternative Methods
RAPID'Salmonella Double Enrichment Protocol RAPID'Salmonella Short Protocol
Detection of Salmonella spp. Detection of Salmonella spp.
Sample Sample
Standard Tests
Standard Tests
or Oxidase/OMNI-O/ONPG
or Oxidase/OMNI-O/ONPG
or iQ-Check Salmonella II
or iQ-Check Salmonella II
or Salmonella Latex Test
Alternative Method
Ordering Information
iQ-Check Salmonella II Real-Time PCR Kit Catalog # Description
Detection of Salmonella spp.
Antiserum Salmonella
Omnivalent OMNI-O (A60)
3560781 3 ml dropper bottle
Dehydrated
3564284 500 g
Dehydrated
3564324 500 g
RAPID'Sakazakii
FDA-BAM
ISO Standard Cronobacter spp. Protocol Alternative Method
ISO/TS 22964:2017 RAPID'Sakazakii Short Protocol
Microbiology of the food chain — Sample
Horizontal method for the
detection of Cronobacter spp. 36°C
24 ± 2 hr
1:10
Buffered Peptone Water
34−38°C 37°C
18 ± 2 hr 18 ± 2 hr
Centrifuge 3,000 x g for 10 min
Selective enrichment Decant supernatant
Transfer 0.1 ml Resuspend pellet in 200 µl PBS
into 10 ml of CSB
41.5°C
24 ± 2 hr Streak onto
RAPID'Sakazakii
Streak onto CCI Spread and
streak plates
41.5°C 100 µl
24 ± 2 hr
Reading and confirmation
Transfer 1 to 5 colonies
on Nonselective Agar
DFI R&F PCR
34−38°C agar agar
21 ± 3 hr Standard Tests
Dehydrated
3564684 500 g
Environmental sample Infant formula iQ-Check Cronobacter spp.
3578137 96 tests
RAPID'Sakazakii Agar
Ready to use
Buffered Peptone Water Supplemented 3563971 90 mm x 20 dishes
Buffered Peptone Water
37°C Dehydrated
80 ± 2 hr 30°C 3564976 500 g
20 ± 2 hr
Trypto-Casein Soy Agar (TSA)
Buffered Peptone Water Ready to use
37°C 3563884 90 mm x 20 dishes
4 ± 1 hr 3555947 200 ml x 6 bottles
3555944 8 ml x 25 inclined tubes
Bacterial lysis and DNA extraction
+ –
Automatic results analysis and interpretation
Sample
Half Fraser
30°C 30°C
24 ± 2 hr 24 ± 2 hr
Primary enrichment Secondary enrichment
Isolation
Fraser 1
(0.1 ml culture/10 ml)
35 or 37°C
48 ± 2 hr
Isolation
Agar Listeria (according Other solid
to Ottaviani and Agosti) selective medium
Listeria spp.
TYSEA agar, catalase, G
ram test, mobility
Listeria monocytogenes
TYSEB, hemolysis, carbohydrates, CAMP test
Sample Sample
BLEB LEB
30°C
4 hr
+ supplements
30°C 30°C 30°C
24 hr 24 hr 48 hr
30°C
24 ± 2 hr
(48 ± 2 hr*) 0.1 ml MFB
Oxford or
modified
or Palcam and or 35°C
Oxford
24 hr (48 hr)
(MOX)
Standard Tests
Motility, hemolysis, mannitol, rhamnose, and
Listeria spp. xylose (or other equivalent compendium-
TYSEA agar, catalase, Gram test, mobility
approved methods)
or Real-time PCR
RAPID'L.mono
Alternative Methods
RAPID'L.mono RAPID'L.mono
Detection of Listeria monocytogenes Enumeration of Listeria
monocytogenes
Sample Sample
Resuscitation
30°C 20°C
24 ± 2 hr 1 hr
37°C 37°C
24 ± 2 hr 24 ± 2 hr
Standard Tests
TSYEB, hemolysis, carbohydrates, CAMP test
or Rhamnose Test
AL (Agar Listeria
according to Ottaviani
Alternative Methods and Agosti)
Sample Sample
Resuscitation
Half Fraser
30°C Buffered Peptone Water
24 ± 2 hr
or Half Fraser
37°C
48 ± 3 hr
Isolation on AL
37°C
24 ± 2 hr
Standard Tests
TSYEB, hemolysis, carbohydrates, CAMP test
Direct enumeration
or Spot on RAPID'L.mono
Gram test, catalase
or Rhamnose Test*
Alternative Method
Ordering Information
iQ-Check Listeria monocytogenes II Real-Time PCR Kit Catalog # Description
Detection of Listeria monocytogenes Buffered Peptone Water Broth
Ready to use
3554179 225 ml x 6 bottles
3555795 3 L x 4 bags
3555790 5 L x 2 bags
Dehydrated
3564684 500 g
iQ-Check
Sample Listeria monocytogenes II Kit
3578124 96 tests
Listeria Special Broth (LSB)
LSB or Half Fraser Ready to use
3555703 225 ml x 6 bottles
30°C 3555793 5 L x 2 bags
23 ± 1 hr (LSB)
or 25 ± 1 hr Dehydrated
(Half Fraser broth and LSB) 3564703 500 g
3564753 5 kg
Bacterial lysis and DNA extraction
RAPID'L.mono Agar
standard (Half Fraser and LSB) or easy (LSB)
Ready to use
3563964 90 mm x 120 dishes
3563694 90 mm x 20 dishes
3555294 Kit: 190 ml agar + 2 suppl.
Dehydrated base
3564293 500 g
Supplement 1
3564294 10 vials
Real-time PCR amplification and detection with Supplement 2
iQ-Check Listeria monocytogenes II 3564746 10 vials
Rhamnose Test
3553669 28 tests
+ –
Automatic results analysis and interpretation
RAPID'Listeria spp.
Alternative Methods
RAPID'L.mono RAPID'Listeria spp.
Detection of Listeria spp. Detection of Listeria spp.
Sample Sample
30°C 30°C
24 ± 2 hr 24 ± 2 hr
37°C 37°C
(24 hr) 24 ± 2 hr
48 hr
Reading and confirmation tests Reading and confirmation tests
Alternative Methods
AL (Agar Listeria according to Ottaviani and Agosti) iQ-Check Listeria spp. Real-Time PCR Kit
Detection of Listeria spp. Detection of Listeria spp.
Sample Sample
Isolation on AL
37°C
24 ± 2 hr Real-time PCR amplification and detection with
iQ-Check Listeria spp.
Reading and confirmation tests
+ –
Automatic results analysis and interpretation
Standard Tests
Ordering Information
Catalog # Description Catalog # Description
Supplement 1
3564745 10 vials
Supplement 2
3564746 10 vials
Shiga Toxin–Producing
Escherichia coli E. coli O157:H7 Other E. coli
(including O157:H7)
RAPID'E.coli O157:H7
Alternative Methods
RAPID'E.coli O157:H7 iQ-Check STEC VirX and SerO Real-Time PCR Kits
Detection of Escherichia coli O157:H7 Detection and isolation of non-O157 Shiga
toxin−producing E. coli (STEC) from ground beef
+ –
Reading and confirmation tests Real-time PCR amplification and detection with
iQ-Check STEC SerO
(Automatic results analysis and interpretation)
Standard Tests
Alternative Method
Ordering Information
iQ-Check E. coli O157:H7 Real-Time PCR Kit Catalog # Description
Detection of Escherichia coli O157:H7
Buffered Peptone Water Broth
Ready to use
3554179 225 ml x 6 bottles
3555795 3 L x 4 bags
3555790 5 L x 2 bags
Dehydrated
3564684 500 g
Sample iQ-Check E. coli O157:H7 Kit
3578114 96 tests
iQ-Check STEC SerO
Buffered Peptone Water 3578140 32 tests
41.5°C iQ-Check STEC VirX
8−24 hr 3578139 96 tests
Campylobacter spp.
ISO Standards
ISO 10272-1:2006 ISO/TS 10272-2:2006
Horizontal method for detection and enumeration of Horizontal method for detection and enumeration of
Campylobacter spp. Campylobacter spp.
Microbiology of food and animal feeding stuffs Microbiology of food and animal feeding stuffs
Part 1: Detection method Part 2: Enumeration method
Sample Sample
Dilution
Incubate
Bacterial lysis and DNA extraction
Microaerobic 42 ± 1°C
48 ± 2 hr
Negative Positive
Can be reported Confirm isolates
as negative and report as positive
Campylobacter spp.
RAPID'Campylobacter
Alternative Methods
RAPID'Campylobacter spp. RAPID'Campylobacter spp.
Detection of Campylobacter spp. Enumeration of Campylobacter spp.
Preston broth
41.5 ± 1°C
24 ± 2 hr
Isolation on RAPID'Campylobacter
or iQ-Check Campylobacter
Standard Tests
or iQ-Check Campylobacter
Ordering Information
Catalog # Description
Dehydrated
3564684 500 g
Confirmation
3563784 Columbia sheep blood agar,
90 mm x 20 dishes
3554164 Physiologic sterile water (0.85%),
9 ml x 25 tubes
3554154 Sterile distilled water,
9 ml x 25 tubes
3564297 Campylobacter Confirm Latex Kit,
50 tests
iQ-Check Campylobacter Kit
3578135 96 tests
RAPID'Campylobacter Agar
Dehydrated base
3564295 500 g
Supplement
3564296 10 vials (10 x 400 ml)
Tryptone Salt Diluent
Ready to use
3555756 90 ml x 6 bottles
3555754 9 ml x 25 tubes
3555747 9 ml x 100 tubes
3555796 3 L x 4 bags
Dehydrated
3564544 500 g
Staphylococcus aureus
ISO Standards
ISO 6888-1/A1:2004 ISO 6888-2/A1:2003
Horizontal method for the enumeration of coagulase- Horizontal method for the enumeration of coagulase-
positive Staphylococci (S. aureus and other species) positive Staphylococci (S. aureus and other species)
Technique including confirmation of colonies Technique using rabbit plasma fibrinogen agar medium
2x*
2x*
Baird-Parker
35 or 37°C
Baird-Parker for RPF
24 ± 2 hr
(+ 24 ± 2 hr)**
+ RPF sup.
Enumeration 35 or 37°C
18−24 hr and 48 hr
Rabbit Plasma
35 or 37°C
4−6 hr or 24 hr
Coagulation reading
FDA BAM
ISO Standard S. aureus Protocol
ISO 6888-3:2003
Horizontal method for the enumeration of coagulase- Sample/initial suspension
positive Staphylococci (S. aureus and species)
Detection and MPN technique for low numbers
Sample/initial suspension
or
Confirmation
37°C 37°C
24 hr 24 hr
Rabbit Plasma
37°C
4–6 hr or 24 hr
Reading
* For detection method only, if low or absent colony growth observed after 24 hr.
Staphylococcus Staphylococcus
coagulase-positive coagulase-negative
RAPID'Staph agar
Alternative Method
Ordering Information
RAPID'Staph Catalog # Description
Enumeration of coagulase-positive Staphylococci
Additives
Egg yolk with potassium tellurite
3554201 5 ml x 1 vial
3554205 25 ml x 1 bottle
Dehydrated
3564014 500 g
Pastorex Staph Plus
™
Pastorex Staph Plus Latex
3556356 50 tests
or Spot on Baird-Parker for RPF 3556353 5 x 50 tests
Rabbit Plasma
Freeze-dried
3556352 Pack for 20 reactions
Staphylocoagulase Broth
53544 9 ml x 25 tubes
RAPID'Staph Agar
Ready to use
3563960 90 mm x 20 dishes
Dehydrated base
3564704 500 g
ISO Standard
ISO 15213:2003
Horizontal method for the enumeration of sulfite-reducing bacteria growing
under anaerobic conditions
Microbiology of food and animal feeding stuffs
Heat treatment
20 min at 75°C
Dilution
2x*
TSC
Confirmation
Clostridium perfringens
Clostridium
perfringens
TSC
ISO Standard
Ordering Information
ISO 7937:2005 Catalog # Description
Horizontal method for the enumeration of Clostridium perfringens
Lactose Sulfite Broth
Colony-count technique Base
3564914 100 g
Columbia Sheep Blood Agar
Ready to use
3563784 90 mm x 20 dishes
Dehydrated base
Food sample/initial suspension 3564674 500 g
Dehydrated
64084 500 g
2x*
TSC without D-Cycloserine Agar
— Base Medium
TSC Ready to use (to be supplemented)
3554419 100 ml x 6 bottles
(add D-cycloserine)
Dehydrated base
Anaerobic 37°C 3569644 500 g
condition 20 ± 2 hr
Mossel (MYP)
ISO Standard
ISO 7932:2005
General guidance for the enumeration of B. cereus
Colony-count technique at 30°C
2x*
Mossel (MYP)
30°C
18–24 hr
(+24 hr)
Confirmation test on
5 well-isolated colonies
30°C
24 ± 2 hr
Shigella
ISO Standard
Ordering Information
ISO 21567:2005 Catalog # Description
Horizontal method for the detection of Shigella spp.
Hektoen Agar
Ready to use
3563894 90 mm x 20 dishes
Dehydrated
3564284 500 g
MacConkey + Crystal Violet Agar
Ready to use
Food sample/initial suspension 3563617 90 mm x 20 dishes
Dehydrated
3569084 500 g
Novobiocin Supplement
Shigella Broth + Novobiocin Dehydrated
pH 7 ± 0.2 3564610 1g
Nutrient (2.1%) Agar
41°C
Dehydrated
16–20 hr
3564485 500 g
37°C
20–24 hr
Campylobacter spp.
Cronobacter spp.
E. coli O157:H7
Listeria monocytogenes
Listeria spp.
Salmonella spp.
Campylobacter spp.
Coliforms
Cronobacter spp.
Escherichia coli
E. coli O157:H7
Listeria monocytogenes
Detection
Enumeration
Listeria spp.
Salmonella spp.
Staphylococcus aureus
* Alternative analytical methods for agrifood — http://nf-validation.afnor.org/en
** USDA No Objection Letter
Approved
Meat,
Poultry, Water
and Fish Dairy Processed Fruits and and Wine
Products Products Foods Vegetables Environment Beverages
Bacillus cereus C C
Campylobacter R iQ R iQ R iQ
Clostridium perfringens C C
Coliforms R R R R R
Cronobacter sakazakii R iQ R iQ
Enterobacteriaceae R R R R R R
Escherichia coli R R R R R R
E. coli O157:H7 R iQ R iQ R iQ R iQ R iQ R iQ
STEC iQ iQ iQ iQ iQ iQ
Lactobacillus C C
Listeria spp. R iQ R iQ R iQ R iQ R iQ R iQ
L. monocytogenes R iQ R iQ R iQ R iQ R iQ R iQ
Pseudomonas
C C C
aeruginosa
Salmonella spp. R iQ R iQ R iQ R iQ R iQ R iQ
S. Enteritidis iQ iQ iQ iQ iQ iQ
S. Typhimurium iQ iQ iQ iQ iQ iQ
Staphyloccocus aureus R R R R R
C Classical media
R RAPID'Chromogenic method
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