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    Dengue Report

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    jed larsen capulong gavino
    The document discusses non-reactive test results on suspected dengue patients using two rapid test kits. It summarizes the procedures for the Bio Tracer Dengue IgG/IgM Rapid Card and SD DENGUE NS1 + Ab Combo kits. Negative results may occur if the quantity of antigens is below detection limits, antigens are not present during sample collection, or if IgM antibodies are too low, especially in early or some secondary infections. The date of examination after exposure, antigen and antibody levels in specimens, and possibility of other diseases need to be considered for non-reactive results.

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    Dengue Report

    Uploaded by

    jed larsen capulong gavino
    539 views1 page
    The document discusses non-reactive test results on suspected dengue patients using two rapid test kits. It summarizes the procedures for the Bio Tracer Dengue IgG/IgM Rapid Card and SD DENGUE NS1 + Ab Combo kits. Negative results may occur if the quantity of antigens is below detection limits, antigens are not present during sample collection, or if IgM antibodies are too low, especially in early or some secondary infections. The date of examination after exposure, antigen and antibody levels in specimens, and possibility of other diseases need to be considered for non-reactive results.

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    The document discusses non-reactive test results on suspected dengue patients using two rapid test kits. It summarizes the procedures for the Bio Tracer Dengue IgG/IgM Rapid Card and SD DENGUE NS1 + Ab Combo kits. Negative results may occur if the quantity of antigens is below detection limits, antigens are not present during sample collection, or if IgM antibodies are too low, especially in early or some secondary infections. The date of examination after exposure, antigen and antibody levels in specimens, and possibility of other diseases need to be considered for non-reactive results.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    539 views1 page

    Dengue Report

    Uploaded by

    jed larsen capulong gavino
    The document discusses non-reactive test results on suspected dengue patients using two rapid test kits. It summarizes the procedures for the Bio Tracer Dengue IgG/IgM Rapid Card and SD DENGUE NS1 + Ab Combo kits. Negative results may occur if the quantity of antigens is below detection limits, antigens are not present during sample collection, or if IgM antibodies are too low, especially in early or some secondary infections. The date of examination after exposure, antigen and antibody levels in specimens, and possibility of other diseases need to be considered for non-reactive results.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    of 1

    LABORATORY DEPARTMENT

    Concern: NON-REACTIVE results on suspected Dengue Patients


    Requested by: Dr. Emy Sangalang

    All request were subjected to proper procedure by the MTOD as required and stated by the inserts of SD
    Dengue NS1 + Ab Combo and Bio Tracer Dengue IgG/IgM Rapid Card.

    Bio Tracer Dengue IgG/IgM Rapid Card Exp Date: 12-2017


    1. Add 5ul of specimen (serum, plasma or whole blood) to sample well
    2. Add 4 drops of sample diluent to diluent well
    3. Read after 15 – 20 minutes
    SD DENGUE NS1 + Ab Combo Exp Date: 01-2018
    NS1
    1. Add 100ul of serum, plasma or whole blood into sample well
    Ab Combo
    1. Add 10ul of serum, plasma or whole blood into sample well marked “S”
    2. Put 4 drops of assay diluent into the round-shaped assay diluent well
    READ after 15-20minutes

    All of this procedure was done properly and accurately by MTODs.


    With regards to the concern of NON-REACTIVE results with marked platelet drops, we can correlate it to the
    indications and principle of the procedures:

    Bio Tracer Dengue IgG/IgM Rapid Card


    Principle: Immunochromatography
    In-vitro test for the qualitative determination of dengue virus specific IgG or IgM
    ***If antibodies against dengue are present enough in the sample, a colored band of dengue IgG/IgM in
    the test zone will appear. If there are no antibodies or not sufficient in the sample, the area will remain colourless.
    The sample continues to move to the control reaction zone and forms a red or purple colour, indicating the test is
    working and result is valid

    SD DENGUE NS1 + Ab Combo


    Principle: Immunochromatography
    In-vitro test designed to detect both Dengue NS1 Dengue virus and differential IgG/IgM
    antibodies for detection in acute dengue infection.
    Limitation of the Test:
    1. A negative result can occur if the quantity of Dengue virus NS1 antigen is present in the
    specimen is below the detection limits of the assay, or the antigens that are detected by the
    test are not present during the stage of disease in which sample is collected
    2. A negative result cannot exclude a recent infection
    3. In early infections and some secondary infections, detectable levels of IgM antibodies may be
    low. Some patients may not produce detectable levels of IgM antibodies within the first seven
    to 10 days after infection. Where symptoms persist, patients should be re-tested 3 – 4 days
    after the first specimen.

    Factors to consider:
    1. Date of examination after disease / antigen exposure
    2. Quantity of antigen and antibodies in the specimen
    3. Other diseases to consider : Chikungunya

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