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    Revisi Valid

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    irfan
    This document contains data from two calibration curves measuring absorbance of lead (Pb) standards at concentrations of 0, 0.25, 0.5, 1 mg/L. The first curve met method acceptance criteria of RSD <8% and recovery of 90-110%. The second curve also met criteria, with recoveries of 104.4% and 100.3%. The document also includes a third calibration curve measuring absorbance of additional Pb standards at 2, 3, 4 mg/L. Statistics for this curve indicate it also meets acceptance criteria.

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    Revisi Valid

    Uploaded by

    irfan
    23 views12 pages
    This document contains data from two calibration curves measuring absorbance of lead (Pb) standards at concentrations of 0, 0.25, 0.5, 1 mg/L. The first curve met method acceptance criteria of RSD <8% and recovery of 90-110%. The second curve also met criteria, with recoveries of 104.4% and 100.3%. The document also includes a third calibration curve measuring absorbance of additional Pb standards at 2, 3, 4 mg/L. Statistics for this curve indicate it also meets acceptance criteria.

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    This document contains data from two calibration curves measuring absorbance of lead (Pb) standards at concentrations of 0, 0.25, 0.5, 1 mg/L. The first curve met method acceptance criteria of RSD <8% and recovery of 90-110%. The second curve also met criteria, with recoveries of 104.4% and 100.3%. The document also includes a third calibration curve measuring absorbance of additional Pb standards at 2, 3, 4 mg/L. Statistics for this curve indicate it also meets acceptance criteria.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    23 views12 pages

    Revisi Valid

    Uploaded by

    irfan
    This document contains data from two calibration curves measuring absorbance of lead (Pb) standards at concentrations of 0, 0.25, 0.5, 1 mg/L. The first curve met method acceptance criteria of RSD <8% and recovery of 90-110%. The second curve also met criteria, with recoveries of 104.4% and 100.3%. The document also includes a third calibration curve measuring absorbance of additional Pb standards at 2, 3, 4 mg/L. Statistics for this curve indicate it also meets acceptance criteria.

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    Data 1

    Std Pb (mg/L) 0 0.25 0.5 1


    Ulangan Abs Conc Abs Conc Abs Conc Abs Conc
    1 0.0006 0.0050 0.0107 0.0197
    2 0.0005 0.0040 0.0115 0.0201
    3 0.0006 0.0030 0.0106 0.0205
    4 0.0007 0.0040 0.0091 0.0189
    5 0.0008 0.0041 0.0105 0.0180
    6 0.0005 0.0040 0.0098 0.0175
    7 0.0004 0.0055 0.0099 0.0187
    X 0.0006 0.0042 0.0103 0.0191
    SD 0.0001 0.0008 0.0008 0.0011
    %RSD 16.67 19.05 7.77 5.76
    %Rec
    √n
    3(SD/√n)
    Syarat Keberterimaan %RSD < 8% dan %Rec (90-110)%
    Batas Atas
    Batas Bawah

    Data 2
    Std Pb (mg/L) 0 0.25 0.5 1
    Ulangan Abs Conc Abs Conc Abs Conc Abs Conc
    1 0.0006 -0.0110 0.0107 0.5440 0.0197 1.0385
    2 0.0005 -0.0165 0.0115 0.5879 0.0201 1.0604
    3 0.0006 -0.0110 0.0106 0.5385 0.0205 1.0824
    4 0.0007 -0.0055 0.0091 0.4560 0.0189 0.9945
    5 0.0008 0.0000 0.0105 0.5330 0.0180 0.9451
    6 0.0005 -0.0165 0.0098 0.4945 0.0175 0.9176
    7 0.0004 -0.0220 0.0099 0.5000 0.0187 0.9835
    X 0.0006 -0.0118 0.0103 0.5220 0.0191 1.0031
    SD 0.0001 0.0074 0.0008 0.0425 0.0011 0.0605
    %RSD 16.67 -62.71 7.77 8.14 5.76 6.03
    %Rec 104.40 100.31
    √n
    3(SD/√n)
    Syarat Keberterimaan %RSD < 8% dan %Rec (90-110)%
    Batas Atas
    Batas Bawah
    LOD Teoritis -0.0115
    LOD Kurva 0.0808
    LOQ Teoritis -0.0108
    Sensitivitas 0.2418
    r 0.9999
    MDL 0.0115
    2 3 4 Deret Standar
    Abs Conc Abs Conc Abs Conc Deret Std Abs
    0.0385 0.0572 0.0733 0.00 0.0006
    0.0371 0.0566 0.0750 0.25 0.0042 0.0800
    0.0366 0.0551 0.0716 0.50 0.0103 0.0700 f(x) = 0.018335058
    0.0600 R² = 0.9995798781
    0.0356 0.0554 0.0731 1.00 0.0191
    0.0361 0.0551 0.0756 2.00 0.0367 0.0500
    0.0365 0.0571 0.0733 3.00 0.0561 0.0400
    0.0364 0.0561 0.0725 4.00 0.0735 0.0300
    0.0367 0.0561 0.0735 r 0.9998 0.0200
    0.0009 0.0009 0.0014 a 0.0005
    0.0100
    2.45 1.60 1.90 b 0.0183
    0.0000
    0.00 0.50 1.00 1.50

    n %Rec (90-110)%

    Deret Standar
    2 3 4 Deret Std Abs (Yi) Yc
    Abs Conc Abs Conc Abs Conc 0.00 0.0006 0.0008
    0.0385 2.0714 0.0572 3.0989 0.0733 3.9835 0.50 0.0103 0.0099
    0.0371 1.9945 0.0566 3.0659 0.0750 4.0769 1.00 0.0191 0.0190
    0.0366 1.9670 0.0551 2.9835 0.0716 3.8901 2.00 0.0367 0.0372
    0.0356 1.9121 0.0554 3.0000 0.0731 3.9725 3.00 0.0561 0.0554
    0.0361 1.9396 0.0551 2.9835 0.0756 4.1099 4.00 0.0735 0.0736
    0.0365 1.9615 0.0571 3.0934 0.0733 3.9835 r 0.9999 SUM
    0.0364 1.9560 0.0561 3.0385 0.0725 3.9396 a 0.0008 Sy/x
    0.0367 1.9717 0.0561 3.0377 0.0735 3.9937 b 0.0182 LOD
    0.0009 0.0507 0.0009 0.0499 0.0014 0.0761 n-2 4
    2.45 2.57 1.60 1.64 1.90 1.91 Abs [Pb] 0.0044
    98.59 101.26 99.84 T Tabel 3.143

    n %Rec (90-110)% Kurva Kalibrasi 0.0800


    Conc Abs 0.0700 f(x) = 0.018
    0.0000 0.0008 R² = 1
    0.0600
    115 0.5000 0.0099
    1.0000 0.0190 0.0500
    08
    Absorbansi

    108 2.0000 0.0372 0.0400


    18 3.0000 0.0554 0.0300
    99 4.0000 0.0736 0.0200
    0.0100
    0.0000
    0.0000 1.0000
    Absorbans
    0.0400
    0.0300
    0.0200
    15 r 1.0000
    0.0100
    a 0.0008
    0.0000
    Kemiringan Kurva Kalibrasi b 0.0182 0.0000 1.0000
    xi yi yi/xi
    0.50 0.0099 0.0198
    1.00 0.0190 0.0190
    2.00 0.0372 0.0186
    3.00 0.0554 0.0185
    4.00 0.0736 0.0184
    rerata 0.0189
    slope 0.0182
    tan α 0.9630
    α 43.92
    Dere Standar
    0.0800
    0.0700 f(x) = 0.0183350582x + 0.0004854463
    0.0600 R² = 0.9995798781

    0.0500 Abs
    Linear (Abs)
    0.0400
    0.0300
    0.0200
    0.0100
    0.0000
    0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50

    (Yi-Yc)2 (Yi-Yc) 0.0800


    0.00000004 -0.0002
    0.0700 f(x) = 0.0182252632x + 0.0008224561
    0.00000016 0.0004 R² = 0.9997653543
    0.0600
    0.00000001 0.0001
    0.00000025 -0.0005 0.0500
    Absorbansi

    0.00000049 0.0007 0.0400


    0.00000001 -0.0001 0.0300 Abs (Yi)
    0.00000096 Linear (Abs (Yi))
    0.0200
    0.0004898979
    0.0100
    0.0807524091
    0.0000
    0.000.501.001.502.002.503.003.504.004.50
    Conc (mg/L)

    0.0800
    0.0700 f(x) = 0.0182x + 0.0008
    0.0600 R² = 1

    0.0500
    Absorbansi

    0.0400
    0.0300 Abs
    Linear (Abs)
    0.0200
    0.0100
    0.0000
    0.0000 1.0000 2.0000 3.0000 4.0000 5.0000
    Absorbans
    0.0400
    0.0300 Abs
    Linear (Abs)
    0.0200
    0.0100
    0.0000
    0.0000 1.0000 2.0000 3.0000 4.0000 5.0000
    Conc (mg/L)
    Data 1
    Std Pb (mg/L) 0 0.25 0.5 1
    Ulangan Abs Conc Abs Conc Abs Conc Abs Conc
    1 0.0006 0.0050 0.0107 0.0197
    2 0.0005 0.0040 0.0115 0.0201
    3 0.0006 0.0030 0.0106 0.0205
    4 0.0007 0.0040 0.0091 0.0189
    5 0.0008 0.0041 0.0105 0.0180
    6 0.0005 0.0040 0.0098 0.0175
    7 0.0004 0.0055 0.0099 0.0187
    X 0.0006 0.0042 0.0103 0.0191
    SD 0.0001 0.0008 0.0008 0.0011
    %RSD 16.67 19.05 7.77 5.76
    %Rec
    √n 2.6458
    3(SD/√n) 0.0001 0.0009 0.0009 0.0012
    Syarat Keberterimaan %RSD < 8% dan %Rec (90-110)%
    Batas Atas 0.0007 0.0051 0.0112 0.0203
    Batas Bawah 0.0005 0.0033 0.0094 0.0179

    Data 2
    Std Pb (mg/L) 0 0.25 0.5 1
    Ulangan Abs Conc Abs Conc Abs Conc Abs Conc
    1 0.0006 0.0107 0.0197
    2 0.0005 0.0201
    3 0.0006 0.0106
    4 0.0007 0.0189
    5 0.0008 0.0105 0.0180
    6 0.0005 0.0098
    7 0.0004 0.0099 0.0187
    X 0.0006 0.0103 0.0191
    SD 0.0001 0.0004 0.0008
    %RSD 16.67 3.88 4.19
    %Rec
    √n 2.4495 2.2361 2.2361
    3(SD/√n) 0.0001 0.0005 0.0011
    Syarat Keberterimaan %RSD < 8% dan %Rec (90-110)%
    Batas Atas 0.0007 0.0108 0.0202
    Batas Bawah 0.0005 0.0098 0.0180

    Data 3 (Data Akhir)


    Std Pb (mg/L) 0 0.25 0.5 1
    Ulangan Abs Conc Abs Conc Abs Conc Abs Conc
    1 0.0006 -0.0166 0.0107 0.5414 0.0197 1.0387
    2 0.0005 -0.0221 0.0201 1.0608
    3 0.0006 -0.0166 0.0106 0.5359
    4 0.0007 -0.0110 0.0189 0.9945
    5 0.0008 -0.0055 0.0105 0.5304 0.0180 0.9448
    6 0.0005 -0.0221 0.0098 0.4917
    7 0.0004 -0.0276 0.0099 0.4972 0.0187 0.9834
    X 0.0006 -0.0174 0.0103 0.5193 0.0191 1.0044
    SD 0.0001 0.0074 0.0004 0.0231 0.0008 0.0460
    %RSD 16.67 -42.53 3.88 4.45 4.19 4.58
    %Rec 103.86 100.44
    √n 2.6458 2.2361 2.0000
    3(SD/√n) 0.0001 0.0005 0.0012
    Syarat Keberterimaan %RSD < 8% dan %Rec (90-110)%
    Batas Atas 0.0007 0.0108 0.0203
    Batas Bawah 0.0005 0.0098 0.0179
    LOD Teoritis -0.0171
    LOD Kurva 0.0816
    LOQ Teoritis -0.0164
    Sensitivitas 0.2431
    r 0.9998
    MDL 0.0059
    2 3 4 Deret Standar
    Abs Conc Abs Conc Abs Conc Deret Std Abs
    0.0385 0.0572 0.0733 0.00 0.0006
    0.0371 0.0566 0.0750 0.25 0.0042
    0.0366 0.0551 0.0716 0.50 0.0103
    0.0356 0.0554 0.0731 1.00 0.0191
    0.0361 0.0551 0.0756 2.00 0.0367
    0.0365 0.0571 0.0733 3.00 0.0561
    0.0364 0.0561 0.0725 4.00 0.0735
    0.0367 0.0561 0.0735 r 0.9998
    0.0009 0.0009 0.0014 a 0.0005
    2.45 1.60 1.90 b 0.0183

    0.0010 0.0010 0.0016


    n %Rec (90-110)%
    0.0377 0.0571 0.0751
    0.0357 0.0551 0.0719

    2 3 4 Deret Standar
    Abs Conc Abs Conc Abs Conc Deret Std Abs
    0.0733 0.00 0.0006
    0.0371 0.0566 0.0750 0.50 0.0103
    0.0366 0.0551 1.00 0.0191
    0.0356 0.0554 0.0731 2.00 0.0364
    0.0361 0.0551 3.00 0.0559
    0.0365 0.0571 0.0733 4.00 0.0734
    0.0364 0.0561 0.0725 r 0.9998
    0.0364 0.0559 0.0734 a 0.0008
    0.0005 0.0008 0.0009 b 0.0182
    1.37 1.43 1.23

    2.4495 2.4495 2.2361


    0.0006 0.0010 0.0012
    n %Rec (90-110)%
    0.0370 0.0569 0.0746
    0.0358 0.0549 0.0722

    ) Deret Standar
    2 3 4 Deret Std Abs (Yi) Yc
    Abs Conc Abs Conc Abs Conc 0.00 0.0006 0.0009
    0.0733 4.0000 0.50 0.0103 0.0100
    0.0566 3.0773 1.00 0.0191 0.0190
    0.0366 1.9724 0.0551 2.9945 2.00 0.0364 0.0371
    0.0554 3.0110 0.0731 3.9890 3.00 0.0557 0.0552
    0.0361 1.9448 0.0551 2.9945 4.00 0.0731 0.0733
    0.0365 1.9669 0.0733 4.0000 r 0.9998 SUM
    0.0364 1.9613 0.0561 3.0497 0.0725 #REF! a 0.0009 Sy/x
    0.0364 1.9614 0.0557 3.0254 0.0731 #REF! b 0.0181 LOD
    0.0002 0.0119 0.0007 0.0367 0.0004 #REF! n-2 4
    0.55 0.61 1.26 1.21 0.55 #REF! Abs [Pb] 0.0044
    98.07 100.85 #REF! T Tabel 3.143
    2.0000 2.2361 2.0000
    0.0003 0.0009 0.0006
    n %Rec (90-110)% Kurva Kalibrasi 0.0800
    0.0367 0.0566 0.0737 Conc Abs 0.0700 f(x) = 0.0
    0.0361 0.0548 0.0725 0.0000 0.0009 R² = 0.99
    0.0600
    171 0.5000 0.0100
    0.0500
    16 1.0000 0.0190

    Absorbansi
    164 2.0000 0.0371 0.0400
    31 3.0000 0.0552 0.0300
    98 4.0000 0.0733 0.0200
    59 r 1.0000 0.0100
    a 0.0009
    0.0000
    Kemiringan Kurva Kalibrasi b 0.0181 0.0000 1.000
    xi yi yi/xi
    0.50 0.0100 0.0200
    1.00 0.0190 0.0190
    2.00 0.0371 0.0186
    3.00 0.0552 0.0184
    4.00 0.0733 0.0183
    Rerata 0.0189
    slope 0.0181
    tan α 0.9577
    α 43.76
    0.0800
    (Yi-Yc)2 (Yi-Yc)
    0.0700 f(x) = 0.0181010526x + 0.0008564912
    0.00000009 -0.0003 R² = 0.9997450879
    0.0600
    0.0500
    bsorbansi

    0.0400
    0.0300 Abs (Yi)
    0.0800
    0.0700 f(x) = 0.0181010526x + 0.0008564912
    0.0600 R² = 0.9997450879
    0.00000009 0.0003
    0.0500
    0.00000001 0.0001

    Absorbansi
    0.00000049 -0.0007 0.0400
    0.00000025 0.0005 0.0300 Abs (Yi)
    Linear (Abs (Yi))
    0.00000004 -0.0002 0.0200
    0.00000097
    0.0100
    0.0004924429
    0.0000
    0.0816203685
    0.000.501.001.502.002.503.003.504.004.50
    Conc (mg/L)

    0.0800
    0.0700 f(x) = 0.0180947368x + 0.0009175439
    0.0600 R² = 0.9999995488

    0.0500
    Absorbansi

    0.0400
    0.0300 Abs
    Linear (Abs)
    0.0200
    0.0100
    0.0000
    0.0000 1.0000 2.0000 3.0000 4.0000 5.0000
    Conc (mg/L)

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