Cambridge International AS and A Level Biology by Mary Jones Revision Guide 2nd Ed PDF

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Cambridge
International AS and A Level
Biology
Second Edition
Mary Jones
Hodder CIE Biology.indd 1 21/10/2015 17:20

Hodder Education, an Hachette UK company, Carmelite House, 50 Victoria Embankment, London EC4Y 0DZ
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© Mary Jones 2015

ISBN 978-1-4718-2887-4
First printed 2015

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Get the most from this book
Everyone has to decide his or her own revision strategy,
but it is essential to review your work, learn it and test your My revision planner
understanding. This Revision Guide will help you to do
that in a planned way, topic by topic. Use the book as the AS topics
Revised Tested Exam
cornerstone of your revision and don’t hesitate to write in it 1 Cell structure ready
7 Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
— personalise your notes and check your progress by ticking 10 Cell structure and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
off each section as you revise. 1 Cell structure

2 Biological molecules
14
17
Carbohydrates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
Lipids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
19 Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
23 Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
3 Enzymes
25 Enzyme mode of action . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
Tick to track your progress Microscopy 27 Factors affecting the rate of enzyme-catalysed reactions . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
Use the revision planner on pages 4 and 5 to plan your 4 Cell membranes and transport
32 Fluid mosaic membranes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
Light microscopes and electron microscopes Revised
revision, topic by topic. Tick each box when you have: 33 Movement of substances into and out of cells
Cells
5 The are the basic units
mitotic cellfrom which living organisms are made. Most cells are
cycle
. . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
very small, and their structures can only be seen by using a microscope.
l revised and understood a topic 39 Division of cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
You will use a light microscope during your AS course. Light rays pass through
6 Nucleic acids and protein synthesis
the specimen on a slide and are focused by an objective lens and an eyepiece
l tested yourself lens. 42
This DNA andaRNA
produces
44 The genetic
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
magnified
codecan
image of the specimen on the retina of your
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
eye. Alternatively, the image be projected onto a screen, or recorded by a
l practised the exam-style questions camera.
7 Transport in plants
48 Structure of transport . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
An electron microscope uses beamstissues
of electrons . . . . . . . . . .rather
. . . . . . . . than
. . . . . . . light
. . . . . . .rays.
. . . . . .The
specimen has to be very

49 Transport thin and must
mechanisms . . . . . . be
. . . . placed
. . . . . . . . . .in
. . .a . . vacuum,
. . . . . . . . . . . . to . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . .allow
You can also keep track of your revision by ticking off each electrons to pass through it. The electrons are focused onto a screen, or onto
8 Transport
photographic film,in mammals
where they form a magnified image of the specimen.
54 The circulatory system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
topic heading in the book. You may find it helpful to add Magnification
58 The heartand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . .resolution
Magnification can be defined as:
your own notes as you work through each topic. 9 Gas exchange and smoking
61 The human
magnification
size of image
= gas exchange system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
actual size of object
62 Cigarette smoking and health . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
This can be rearranged to:
10 Infectious size
disease
of image
size object = infectious diseases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
64 ofImportantmagnification
68 Antibiotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
There is no limit to the amount you can magnify an image. However, the
Magnification is the size of an image
11 Immunity
amount of useful magnification depends on the resolution of the microscope.
divided by the size of the actual object.
This 69
is theThe
ability of the microscope
immune system . . . . . .to
. . . distinguish
. . . . . . . . . . . . . . .two
. . . . . . objects
. . . . . . . . . . as
. . . .separate . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . from
one another. The smaller thevaccination
objects that . . can be distinguished, the higher the Resolution is the size of the smallest
71 Antibodies and . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
objects that can be distinguished.
resolution. Resolution is determined by the wavelength of the rays that are being
AS experimental
used to view the specimen.skills and investigations
The wavelength of a beam of electrons is much
75 than
smaller Skills
theand mark allocations
wavelength of light. An . . electron . . . . . . . . . . . .microscope
. . . . . . . . . . . . . . . .can . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . therefore
distinguish
75 The between much
practical smaller objects
examination questions than a light . . . . . .microscope
. . . . . . . . . . . . . . . .— . . . .in . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . .other
words,
76anHow electron microscope
to do has practical
well in the a much higher examresolution . . . . . . . . . . . . . than . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . .a . . light
microscope. We can therefore see much more fine detail of a cell using an
91 AS microscope
electron exam-style questions
than using a light microscope. and answers
Features to help you succeed 4

As cells are very small, we have to use units much smaller than millimetres to
measure them. These units are micrometres, µm, and nanometres, nm.
Cambridge 1 × 10−3 m
1 mm = International
1 µm = 1 × 10−6 m
AS and A Level Biology Revision Guide Expert tip
It is almost always a good idea to
1 nm = 1 × 10 m −9 convert every measurement to µm
when doing magnification calculations.
To change mm into µm, multiply by 1000.
Expert tips Exam-style questions

Throughout the book there are tips from the experts on Exam-style questions are provided for AS and A level. Use
Cell structure 7
how to maximise your chances. them to consolidate your revision and practise
your exam skills.
Typical mistakes
Now test yourself
Advice is given on how to avoid the typical mistakes
students often make. These short, knowledge-based questions provide the first
step in testing your learning. Answers are at the back of
the book.
Definitions and key words
Clear, concise definitions of essential key terms are
Revision activities
provided on the page where they first appear.
These activities will help you to understand each topic in
Key words from the syllabus are highlighted in bold for
an interactive way.
you throughout the book.

My revision planner
AS topics
Revised Tested Exam
1 Cell structure ready
7 Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
10 Cell structure and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
14 Carbohydrates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
17 Lipids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
19 Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
23 Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
3 Enzymes
25 Enzyme mode of action . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
27 Factors affecting the rate of enzyme-catalysed reactions . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
4 Cell membranes and transport
32 Fluid mosaic membranes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
33 Movement of substances into and out of cells . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
5 The mitotic cell cycle
39 Division of cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
42 DNA and RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
44 The genetic code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
48 Structure of transport tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
49 Transport mechanisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
8 Transport in mammals
54 The circulatory system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
58 The heart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
9 Gas exchange and smoking
61 The human gas exchange system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
62 Cigarette smoking and health . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
10 Infectious disease
64 Important infectious diseases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
68 Antibiotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
11 Immunity
69 The immune system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
71 Antibodies and vaccination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
AS experimental skills and investigations
75 Skills and mark allocations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
75 The practical examination questions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
76 How to do well in the practical exam . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
. . . . . . . . . . . . . . . . . ■
91 AS exam-style questions and answers
4 Cambridge International AS and A Level Biology Revision Guide

A level topics
Revised Tested Exam
12 Energy and respiration ready
100 Energy in living organisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
101 Respiration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
13 Photosynthesis
109 An overview of photosynthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
114 Limiting factors in photosynthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
116 Leaf structure in C3 and C4 plants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
14 Homeostasis
118 Homeostasis in mammals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
121 The roles of the kidneys in homeostasis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
127 Homeostasis in plants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
15 Control and coordination
128 Control and coordination in animals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
135 Control and coordination in plants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
16 Inherited change
137 Meiosis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
142 Genetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
152 Control of gene expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
17 Selection and evolution
154 Variation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
155 Natural and artificial selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
160 Evolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
18 Biodiversity, classification and conservation
162 Biodiversity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
164 Classification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
165 Conservation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
19 Genetic technology
169 Tools for genetic technology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
174 Genetic technology applied to medicine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
178 Genetically modified organisms in agriculture . . . . . . . . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■ . . . . . . . . . . . . . . . . . ■
A level experimental skills and investigations
180 Skills and mark allocations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .....................
180 How to make the most of your practical skills . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ ■ . . . . . . . . . . . . . . . . . ■

192 A level exam-style questions and answers
202 Now test yourself answers
My Revision Planner 5

Countdown to my exams
6–8 weeks to go 1 week to go
l Start by looking at the syllabus — make sure you l Try to fit in at least one more timed practice of
know exactly what material you need to revise an entire past paper and seek feedback from your
and the style of the examination. Use the revision teacher, comparing your work closely with the
planner on pages 4 and 5 to familiarise yourself mark scheme.
with the topics. l Check the revision planner to make sure you
l Organise your notes, making sure you have haven’t missed out any topics. Brush up on any
covered everything on the syllabus. The revision areas of difficulty by talking them over with a
planner will help you to group your notes into friend or getting help from your teacher.
topics. l Attend any revision classes put on by your
l Work out a realistic revision plan that will allow teacher. Remember, he or she is an expert at
you time for relaxation. Set aside days and times preparing people for examinations.
for all the subjects that you need to study, and Revised
stick to your timetable.
l Set yourself sensible targets. Break your revision The day before the examination
down into focused sessions of around 40 minutes,
divided by breaks. This Revision Guide organises l Flick through this Revision Guide for useful
the basic facts into short, memorable sections to reminders, for example the expert tips, typical
make revising easier. mistakes and key terms.
Revised l Check the time and place of your examination.
l Make sure you have everything you need — extra
pens and pencils, tissues, a watch, bottled water,
sweets.
l Allow some time to relax and have an early
night to ensure you are fresh and alert for the
2–5 weeks to go examinations.
Revised
l Read through the relevant sections of this book
and refer to the expert tips, typical mistakes and My exams
key terms. Tick off the topics as you feel confident
about them. Highlight those topics you find Paper 1
difficult and look at them again in detail. Date: . . . . . . . . . . . . . . . . . . . . . . Time: . . . . . . . . . . . . . . . . . .
l Test your understanding of each topic by working Location: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
through the ‘Now test yourself’ questions in the
book. Look up the answers at the back of the Paper 2
book. Date: . . . . . . . . . . . . . . . . . . . . . . Time: . . . . . . . . . . . . . . . . . .
l Make a note of any problem areas as you revise, Location: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
and ask your teacher to go over these in class.
l Look at past papers. They are one of the best Paper 3
ways to revise and practise your exam skills. Write Date: . . . . . . . . . . . . . . . . . . . . . . Time: . . . . . . . . . . . . . . . . . .
or prepare planned answers to the exam-style
Location: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
questions provided in this book. Check your
answers with your teacher. Paper 4
l Try different revision methods. For example, you Date: . . . . . . . . . . . . . . . . . . . . . . Time: . . . . . . . . . . . . . . . . . .
can make notes using mind maps, spider diagrams Location: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
or flash cards.
l Track your progress using the revision planner and Paper 5
give yourself a reward when you have achieved Date: . . . . . . . . . . . . . . . . . . . . . . Time: . . . . . . . . . . . . . . . . . .
your target. Location: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Revised

1 Cell structure
Microscopy
Light microscopes and electron microscopes Revised
Cells are the basic units from which living organisms are made. Most cells are
very small, and their structures can only be seen by using a microscope.
You will use a light microscope during your AS course. Light rays pass through
the specimen on a slide and are focused by an objective lens and an eyepiece
lens. This produces a magnified image of the specimen on the retina of your
eye. Alternatively, the image can be projected onto a screen, or recorded by a
camera.
An electron microscope uses beams of electrons rather than light rays. The
specimen has to be very thin and must be placed in a vacuum, to allow
electrons to pass through it. The electrons are focused onto a screen, or onto
photographic film, where they form a magnified image of the specimen.
Magnification and resolution

Magnification can be defined as:
size of image
magnification =
This can be rearranged to:
size of image
size of object =
magnification
There is no limit to the amount you can magnify an image. However, the
Magnification is the size of an image
amount of useful magnification depends on the resolution of the microscope.
divided by the size of the actual object.
This is the ability of the microscope to distinguish two objects as separate from
one another. The smaller the objects that can be distinguished, the higher the Resolution is the size of the smallest
objects that can be distinguished.
resolution. Resolution is determined by the wavelength of the rays that are being
used to view the specimen. The wavelength of a beam of electrons is much
smaller than the wavelength of light. An electron microscope can therefore
distinguish between much smaller objects than a light microscope — in other
words, an electron microscope has a much higher resolution than a light
microscope. We can therefore see much more fine detail of a cell using an
electron microscope than using a light microscope.
As cells are very small, we have to use units much smaller than millimetres to
measure them. These units are micrometres, µm, and nanometres, nm.
1 mm = 1 × 10−3 m Expert tip
1 µm = 1 × 10−6 m It is almost always a good idea to
1 nm = 1 × 10−9 m convert every measurement to µm
when doing magnification calculations.
To change mm into µm, multiply by 1000.
Cell structure 7

1 Cell structure
How to… Calculate magnification
You should be able to work out the real size of an object if you are told how much it has been magnified.
For example, the drawing of a mitochondrion in Figure 1.1 has been magnified 100 000 times.
Figure 1.1
l Use your ruler to measure its length in mm. It is 50 mm long.
l As it is a very small object, convert this measurement to µm by multiplying by 1000:
50 × 1000 = 50 000 µm
l Substitute into the equation:
size of image
actual size of object =
magnification
50 000
=
100 000
= 0.5 µm
You can also use a scale bar to do a similar calculation for the drawing of a chloroplast in Figure 1.2.
2 µm
Figure 1.2
l Measure the length of the scale bar.
l Calculate its magnification using the formula
size of image Expert tip

magnification =
Always show every small step in your
length of scale bar working when you do a calculation on
=
length the scale bar represents an exam paper, as there may be marks
200 000 for this.
=
2
= ×100 000
l Measure the length of the image of the chloroplast in mm, and convert to µm. You should find that it is 80 000 µm long.
l Calculate its real length using the formula
size of image
magnification
80 000
=
10 000
= 8 µm
Now test yourself Tested
1 A micrograph shows a chloroplast that measures 79 mm long. The magnification of the micrograph is ×16 000. Calculate
the length of the chloroplast in µm.
Answer on p.202

1 Cell structure
How to… Measure cells using a graticule
An eyepiece graticule is a little scale bar that you can place

in the eyepiece of your light microscope. When you look Eyepiece
down the microscope, you can see the graticule as well as the graticule scale
specimen.
The graticule is marked off in ‘graticule units’, so you can use
the graticule to measure the specimen you are viewing in these
graticule units. Just turn the eyepiece so that the graticule scale
lies over the object you want to measure. It will look like
Figure 1.3.
Stage
micrometer scale
Figure 1.4
l You can see that the 50 mark on the stage micrometer is
lined up with the 1.0 mark on the eyepiece graticule. Work

along towards the right until you see another two lines that
are exactly lined up. There is a good alignment of 68 on the
stage micrometer and 9.0 on the eyepiece graticule. So you
can say that:
80 small eyepiece graticule markings = 18 stage
micrometer markings
Figure 1.3
= 18 × 0.01 mm = 0.18 mm
We can say that the width of one cell is 23 graticule units.
= 180 µm
The graticule units have to be converted to real units, such
as mm or µm. This is called calibration. To do this, you use 180
so 1 small eyepiece graticule marking = = 2.25 µm.
a special slide called a stage micrometer that is marked off 80
in a tiny scale. There should be information on the slide that Now we can calculate the real width of the plant cell we
tells you the units in which it has been marked. The smallest measured. It was 23 eyepiece graticule units long. So its real
markings are often 0.01 mm apart — that is, 10 µm apart. width is:
Take the specimen off the stage or the microscope and replace 23 × 2.25 = 51.75 µm
it with the stage micrometer. Focus on it using the same If you want to look at something using a different objective
objective lens as you used for viewing the specimen. lens, you will have to do the calibration of eyepiece graticule
Line up the micrometer scale and the eyepiece graticule scale. units all over again using this lens. Once you have done it, you
You can do this by turning the eyepiece, and by moving the can save your calibrations for the next time you use the same
micrometer on the stage. Make sure that two large markings on microscope with the same eyepiece graticule and the same
each scale are exactly lined up with each other. You should be objective lens.
able to see something like Figure 1.4.
2 A cell measures 84 small eyepiece graticule units.

When a stage micrometer is placed on the stage using the same objective lens, it can be seen that 100 eyepiece graticule
units exactly line up with 8 stage micrometer markings. The stage micrometer is marked off in 0.01 mm divisions.
Calculate the size of the cell in µm.
Answer on p.202
Cell structure 9

Cell structure and function
1 Cell structure
Figures 1.5 and 1.6 show a typical animal cell and a typical plant cell as seen
using an electron microscope.
Lysosomes Golgi body Nucleus Nucleolus Cell surface Rough endoplasmic

membrane reticulum (RER)
Nuclear
envelope
Small circular
DNA
Mitochondrion Centrioles 80S 70S Smooth endoplasmic

ribosomes ribosomes reticulum (SER)
Figure 1.5 A typical animal cell, ×2000
Rough endoplasmic Nucleus Nucleolus Smooth endoplasmic

reticulum (RER) reticulum (SER)
80S ribosomes
Cell wall
Cell surface
membrane
Chloroplast
70S
Small circular DNA
ribosome
Plasmodesma Mitochondrion Golgi body Nuclear envelope

Figure 1.6 A typical plant cell, ×1500
Functions of membrane systems and organelles Revised
The cell surface membrane controls what enters and leaves the cell. Its structure
and functions are described in detail on pp. 32–33. There are also many
membranes within the cell that help to make different compartments in which
different chemical reactions can take place without interfering with one another.
The nucleus is surrounded by a pair of membranes, which make up the
nuclear envelope. The nucleus contains chromosomes, each of which
contains a very long molecule of DNA. The DNA determines the sequences
in which amino acids are linked together in the cytoplasm to form protein
molecules. This is described on pp. 44–45.
Within the nucleus there is a darker area (not surrounded by its own membrane)
called the nucleolus. This is where new ribosomes are made, following a code
on part of the DNA.
Ribosomes are small structures made of RNA and protein. They are found free
in the cytoplasm, and also attached to rough endoplasmic reticulum (RER).
These ribosomes are 80S (that is, larger than those in the mitochondria and
chloroplasts). The RER is an extensive network of membranes in the cytoplasm.
The membranes enclose small spaces called cisternae. Proteins are made on the
ribosomes, by linking together amino acids.

If the proteins are to be processed or exported from the cell, the growing
1 Cell structure
chains of amino acids move into the cisternae of the RER as they are made. The
cisternae then break off to form little vesicles that travel to the Golgi body.
Here they may be modified, for example by adding carbohydrate groups to
them. Vesicles containing the modified proteins break away from the Golgi body
and are transported to the cell surface membrane (Figure 1.7), where they are
secreted from the cell by exocytosis (p. 38).
2 Vesicles containing the proteins 3 Vesicles containing 4 Vesicles fuse with the cell
fuse to the Golgi body, where modified proteins break surface membrane, releasing
the proteins are modified away from the Golgi body the proteins outside the cell
1 Proteins are made

by ribosomes on the
rough endoplasmic
reticulum and are Cell surface
pinched off into membrane
vesicles
Cytoplasm
Figure 1.7 The interrelationship between RER and the Golgi body
Smooth endoplasmic reticulum (SER) is usually less extensive than RER. It
does not have ribosomes attached to it, and the cisternae are usually more
flattened than those of the RER. It is involved in the synthesis of steroid
hormones and the breakdown of toxins.
Mitochondria have an envelope (two membranes) surrounding them (Figure 1.8).
The inner one is folded to form cristae. This is where aerobic respiration takes
place, producing ATP (see p. 12). The first stage of this process, called the Krebs
cycle, takes place in the matrix. The final stage, oxidative phosphorylation, takes
place on the membranes of the cristae. Mitochondria contain ribosomes that are
smaller than those in the cytoplasm (70S) and a small circular molecule of DNA.
Envelope
Outer membrane Inner membrane Intermembranal

folded into cristae space
Matrix
ATP
synthases
70S ribosomes Small circular DNA 0.1 µm

Figure 1.8 Longitudinal section through a mitochondrion
Lysosomes are little membrane-bound packages of hydrolytic (digestive)
enzymes. They form by breaking off from the Golgi body. They are used to
digest bacteria or other cells taken into the cell by phagocytosis, or to break
down unwanted or damaged organelles within the cell.
Centrioles are found only in animal cells, not plant cells. They are made of tiny
structures called microtubules, arranged in a circular pattern. Microtubules
are made of a protein called actin. The two centrioles lie at right angles to one
another. It is from here that the microtubules are made that form the spindle
during cell division in animal cells. Microtubules are also found throughout the
cell even when it is not dividing, where they help to form the cytoskeleton,
which keeps the cell in shape.
Cell structure 11

Chloroplasts are found only in some plant cells. Like mitochondria, chloroplasts
1 Cell structure
are surrounded by an envelope made up of two membranes (Figure 1.9).

Their background material is called the stroma, which contains many paired
membranes called thylakoids. In places, these form stacks called grana.
The grana contain chlorophyll, which absorbs energy from sunlight. The
first reactions in photosynthesis, called the light dependent reactions and
photophosphorylation, take place on the membranes. The final stages, called
the Calvin cycle, take place in the stroma. Chloroplasts often contain starch
grains, which are storage materials formed from the sugars that are produced in
photosynthesis. Like mitochondria, they contain 70S ribosomes and a circular
DNA molecule.
70S ribosomes Stroma Outer membrane

Envelope
Inner membrane
Starch grain
Lamella
Thylakoid
Revision activity
Cut out 21 pieces of card, all the same
size. On one side of each card write
one of the words shown in bold on
pp. 10–12. On the other side, write no
more than six words that describe the
Granum Small circular DNA Lipid droplet
named structure. Arrange all the cards
Figure 1.9 Longitudinal section through a chloroplast with either the name or the description
visible, and practise identifying what is
Plant cells are always surrounded by a cell wall made of cellulose, which is never on the non-visible side.
found around animal cells. The structure of cellulose is described on p. 16–17.
ATP
Cells constantly use energy to maintain the processes that keep them alive.
These processes include active transport of substances across membranes
(p. 37), movement and the building up of large molecules (such as the synthesis
of proteins, p. 44). The immediate source of energy for a cell is adenosine
triphosphate, or ATP. ATP can be broken down to adenosine diphosphate (ADP)
and a phosphate ion, releasing a small packet of useable energy. Mitochondria
make ATP by aerobic respiration (p. 101), while chloroplasts make ATP in the
light dependent stage of photosynthesis (p. 109).
Prokaryotic cells Revised
Prokaryotic cells are found in bacteria and archaea, whereas eukaryotic cells are
found in animals, plants, protoctista and fungi. Prokaryotic cells are generally much
smaller than eukaryotic cells. The fundamental difference between prokaryotic
and eukaryotic cells is that they do not have a nucleus or any other organelles
bound by a double membrane (Figure 1.10). Most prokaryotic organisms are
unicellular. Eukaryotic organisms (including plants, animals and fungi) are usually
multicellular. Prokaryotic and eukaryotic cells are compared in Table 1.1.
Cell wall made of Cytoplasm

peptidoglycans
Circular DNA
Cell surface membrane
Plasmid
0.1 µm 70S ribosomes

Figure 1.10 Structure of a prokaryotic cell

Table 1.1 Comparison of prokaryotic, animal and plant cells
1 Cell structure
Eukaryotic cells
Feature Prokaryotic cells Animal cells Plant cells
Cell surface membrane Always present Always present Always present
Cell wall Always present; made up of Never present Always present; made up of
peptidoglycans cellulose
Nucleus and nuclear Never present Always present Always present
envelope
Chromosomes Contain so-called ‘bacterial Contain several chromosomes, Contain several
chromosomes’ — a circular each made up of a linear DNA chromosomes, each made
molecule of DNA not associated molecule associated with up of a linear DNA molecule
with histones (sometimes said histones associated with histones
to be ‘naked’ DNA); bacteria
may also contain smaller circles
of DNA called plasmids
Mitochondria Never present Usually present Usually present
Chloroplasts Never present, though some Never present Sometimes present
do contain chlorophyll or other
photosynthetic pigments
Rough and smooth Never present Usually present Usually present
endoplasmic reticulum
and Golgi bodies
Ribosomes 70S ribosomes present 80S ribosomes present in 80S ribosomes present in
cytoplasm; 70S ribosomes in cytoplasm; 70S ribosomes in
mitochondria mitochondria and chloroplasts
Centrioles Never present Usually present Never present
Revision activity
l Using a good quality HB pencil, make a large diagram of a prokaryotic cell.
Check your diagram against Figure 1.10.
l Now convert the prokaryotic cell to an animal cell, by erasing some
structures and adding others. Check your diagram against Figure 1.5.
l Now convert the animal cell to a plant cell, by erasing one structure and
adding others. Check your diagram against Figure 1.6.
Viruses Revised
Viruses are extremely small particles of RNA or DNA surrounded by a protein

coat (Figure 1.11). They are non-cellular. Viruses are not able to carry out any of
the processes characteristic of living things unless they are inside a living cell,
where they ‘hijack’ the cell’s machinery to make copies of themselves. All viruses
are, therefore, parasitic. Some viruses cause disease in humans.
Protein coat
Now test yourself
RNA or DNA 3 Use the scale bar to calculate the
diameter of the virus in Figure 1.11.
Then work out how many viruses
could line up inside the bacterium
shown in Figure 1.10.
Answer on p.202
1 nm Tested
Figure 1.11 Structure of a virus
Cell structure 13

Carbohydrates
Carbohydrates are substances whose molecules contain carbon, hydrogen and
oxygen atoms, and in which there are approximately twice as many hydrogen
atoms as carbon or oxygen atoms.
Monosaccharides and disaccharides Revised
The simplest carbohydrates are monosaccharides. These are sugars. They

include glucose, fructose and galactose. These three monosaccharides each
have six carbon atoms, so they are also known as hexose sugars. Their molecular
formula is C6H12O6.
Monosaccharide molecules are often in the form of a ring made up of carbon
atoms and one oxygen atom. Glucose molecules can take up two different
forms, called α-glucose and β-glucose (Figure 2.1).
6
α-glucose CH2OH
5
C O The hydrogen on
H H carbon 1 is above
4 H 1
C C the plane of the ring
OH H
HO 3 2 OH
C C
H OH
6
β-glucose CH2OH
5
C O Expert tip
H OH
4 H 1 When you are drawing biological
C OH H C molecules, count the bonds you have
The hydrogen on
HO 3 2 H carbon 1 is below
drawn on each atom. Each C should
C C have four bonds, each N should have
the plane of the ring
H OH 3, each O should have 2 and each H
should have 1.
Figure 2.1 Structural formulae of α-glucose and β-glucose molecules
Two monosaccharides can link together to form a disaccharide. For example,
two glucose molecules can link to produce maltose. The bond that joins them
together is called a glycosidic bond. As the two monosaccharides react and the
glycosidic bond forms, a molecule of water is released. This type of reaction is
known as a condensation reaction. Different disaccharides can be formed by
linking different monosaccharides (Table 2.1 and Figures 2.2 and 2.3).
Table 2.1
Disaccharide Monosaccharides
Maltose Glucose + Glucose
Lactose Glucose + Galactose
Sucrose Glucose + Fructose

CH2OH CH2OH
C O C O
H H H H
H + H
C C C C
OH H OH H
HO OH HO OH
C C C C
H OH H OH
CH2OH CH2OH
C O C O
H H H H
H2O + H H
C C C C
OH H OH H
HO OH
C C O C C
H OH H OH
Glycosidic bond
Figure 2.2 Formation of maltose by a condensation reaction
CH2OH
C O
H H HOCH2 O H
H +
C C C C
OH H H HO
HO OH HO CH2OH
C C C C
H OH OH H
CH2OH
C O
H H HOCH2 O H
H2O + H
C C C C
OH H H HO
HO CH2OH
C C O C C
H OH OH H
Glycosidic bond
Figure 2.3 Formation of sucrose by a condensation reaction

Expert tip
Disaccharides can be split apart into two monosaccharides by breaking the
glycosidic bond. To do this, a molecule of water is added. This is called a Remember: carbohydrates are
transported as glucose in animals, but
hydrolysis reaction (Figure 2.4). as sucrose in plants.
H2O + +
Now test yourself
Figure 2.4 Breakdown of a disaccharide by a hydrolysis reaction
1 For each of these sugars, state:
Functions of monosaccharides and disaccharides a whether it is a monosaccharide
l Monosaccharides and disaccharides are good sources of energy in living or a disaccharide
organisms. They can be used in respiration, in which the energy they contain b if a disaccharide, the
monosaccharides from which
is used to make ATP.
it is formed
l Because they are soluble, they are the form in which carbohydrates are
c whether it will give a positive
transported through an organism’s body. In animals, glucose is transported result with the Benedict’s test
dissolved in blood plasma. In plants, sucrose is transported in phloem sap. fructose glucose lactose
All monosaccharides and some disaccharides act as reducing agents, and will maltose sucrose
reduce blue Benedict’s solution to produce an orange-red precipitate. They are Answer on p.202
called reducing sugars. Sucrose is a non-reducing sugar. Tested
Biological molecules 15

Polysaccharides
Revised
These are substances whose molecules contain hundreds or thousands of

monosaccharides linked together into long chains. The monosaccharides are A monomer is a relatively small
monomers, and the polysaccharide is a polymer. Polysaccharides are molecule that can be linked to others
macromolecules. Because their molecules are so enormous, the majority do like it, to form a much longer molecule.
not dissolve in water. This makes them good for storing energy (starch and A polymer is a molecule made up of
glycogen) or for forming strong structures (cellulose). many smaller molecules (monomers)
linked together in a long line.
Storage polysaccharides A macromolecule is a large biological
In animals and fungi, the storage polysaccharide is glycogen. It is made of molecule, such as a polysaccharide
α-glucose molecules linked together by glycosidic bonds. Most of the glycosidic or protein.
bonds are between carbon 1 on one glucose and carbon 4 on the next, so
they are called 1–4 links. There are also some 1–6 links, which form branches
in the chain (Figure 2.5). When needed, the glycosidic bonds can be hydrolysed
by carbohydrase enzymes to form monosaccharides, which can be used in
respiration. The branches mean there are many ‘ends’, which increases the rate at
which carbohydrases can hydrolyse the molecules.
1–4 link 1–6 link
Figure 2.5 A small part of a glycogen molecule

In plants, the storage polysaccharide is starch. Starch is a mixture of two Typical mistake
substances, amylose and amylopectin. An amylose molecule is a long chain of It’s important to remember that you
α-glucose molecules with 1–4 links. It coils up into a spiral, making it very do not find starch in animal cells, nor
compact. The spiral is held in shape by hydrogen bonds (see p. 23) between glycogen in plant cells.
small charges on some of the hydrogen and oxygen atoms in the glucose units
(Figure 2.6). An amylopectin molecule is similar to glycogen.
Amylose contains 6 6 6
chains of α 1– 4 CH2OH CH2OH CH2OH
5 5 5
linked glucose C O C O C O
H H H H H H
4C
H 1 H 1 H 1
C 4C C 4C C
OH H OH H OH H
2 2 2
O 3C C O 3C C O 3C C
H OH H OH H OH
The glucose molecules
in a chain all have the α 1– 4 Glycosidic bond
same orientation
1– 4 link
Hydrogen bond — many
of these hold the amylose
A monosaccharide is a single sugar
in a spiral shape
unit, with the formula CnH2nOn, e.g.
Figure 2.6 A small part of an amylose molecule glucose.
A disaccharide is a substance made of
Structural polysaccharides two monosaccharide molecules linked
Plant cell walls contain the polysaccharide cellulose. Like amylose, this is made by a glycosidic bond, e.g. maltose.
of many glucose molecules linked by glycosidic bonds between carbon 1 and
A polysaccharide is a substance made
carbon 4 (Figure 2.7). However, in cellulose the glucose molecules are in the
of many monosaccharide molecules
β form. This means that adjacent glucose molecules in the chain are upside- linked in a long chain, e.g. glycogen.
down in relation to one another. The chain stays straight, rather than spiralling.

Hydrogen bonds form between different chains (Figure 2.8). This causes the
chains to associate into bundles called microfibrils.
β 1– 4 Glycosidic bond
6 6
CH2OH H OH CH2OH
5
C O O 3
C 2
C 5
C O
H H H
H 1C C OH H 1 H 1
4C C 4 C 4C C
OH H H OH H
2 H H 2 H
3C C 5C O O 3C C
6
H OH CH2OH H OH
The glucose molecules in a chain alternate in their

orientation (look at the numbered carbon atoms)
Figure 2.7 Cellulose contains chains of β 1–4 linked glucose
Parallel chains of
β 1– 4 linked glucose
Hydrogen bonds hold

the chains together to
form microfibrils
Now test yourself
Figure 2.8 Cellulose molecules
2 Explain why cellulose works well
The resulting microfibrils are very strong. This makes cellulose an excellent as a structural polysaccharide,
material for plant cell walls, because it will not break easily if the plant cell swells whereas starch does not.
as it absorbs water. The microfibrils are also very difficult to digest, because few Answer on p.202
organisms have an enzyme that can break the β 1–4 glycosidic bonds. Tested
Tests for carbohydrates Revised
Reducing sugar
Add Benedict’s reagent and heat. An orange-red precipitate indicates the
presence of reducing sugar. If standard volumes of the unknown solutions and
excess Benedict’s reagent are used, the mass of precipitate or intensity of the
orange-red colour indicates the concentration of the solution. This can be
matched against colour standards, prepared using reducing sugar solutions of
known concentration. (See p. 78 for an explanation of how to make solutions of
known concentration.)
Non-reducing sugar Now test yourself

This test should only be done on solutions known not to contain reducing 3 Imagine you have been given a
sugars. Some disaccharides, for example sucrose, are non-reducing sugars. sugar solution. You know that it
Hydrolyse (break the glycosidic bond) by heating with dilute HCl, then neutralise contains reducing sugar, but you
with sodium hydrogencarbonate. Then carry out the test for reducing sugar. want to know if it also contains
non-reducing sugar. How could you
Starch find out?
Add iodine in potassium iodide solution. A blue-black colour indicates the Answer on p.202
presence of starch. Tested
Lipids
Lipids, like carbohydrates, also contain carbon, hydrogen and oxygen, but
there is a much smaller proportion of oxygen. Lipids include triglycerides and
phospholipids. All lipids are insoluble in water.

Triglycerides
Revised
A triglyceride molecule is made of a ‘backbone’ of glycerol, to which three fatty

acids are attached by ester bonds (Figure 2.9).
Fatty acid molecule Glycerol molecule Ester bond

H H H H H H H H H
Condensation H
C C O C C O
reaction
C C C OH HO C H
C C C O C H
H H H H HO C H H H H H
HO C H
H2O HO C H
HO C H
Each of the fatty acids is joined
H
to the glycerol by an ester bond. H
Ester bond
A triglyceride has three fatty

acids with three ester bonds.
Figure 2.9 The formation of a triglyceride molecule
Fatty acids have long chains made of carbon and hydrogen atoms. Each carbon
atom has four bonds. Usually, two of these bonds are attached to other carbon
atoms, and the other two to hydrogen atoms. In some cases, however, there
may be only one hydrogen atom attached. This leaves the carbon atom with a
‘spare’ bond, which attaches to the next-door carbon atom (which also has one
less hydrogen bonded to it), forming a double bond. Fatty acids with one or
more carbon-carbon double bonds are called unsaturated fatty acids, because
they do not contain quite as much hydrogen as they could. Fatty acids with no
double bonds are called saturated fatty acids (Figure 2.10).
An unsaturated fatty acid A saturated fatty acid

H H H H
H H C C O
C
C H C C C OH
H H H H H H H
C C O
H
C C OH
Double bond H
Figure 2.10 Unsaturated and saturated fatty acids

Lipids containing unsaturated fatty acids are called unsaturated lipids, and those
containing completely saturated fatty acids are called saturated lipids. Animal
lipids are often saturated lipids. Plant lipids are often unsaturated. Unsaturated
lipids tend to have lower melting points than saturated lipids.
Triglycerides are used as energy storage compounds in plants, animals and fungi.
Their insolubility in water helps to make them suitable for this function. They
contain more energy per gram than polysaccharides, so can store more energy
in less mass. In mammals, stores of triglycerides often build up beneath the skin,
in the form of adipose tissue. The cells in adipose tissue contain oil droplets
made up of triglycerides.
This tissue also helps to insulate the body against heat loss. It is a relatively
low-density tissue, and therefore increases buoyancy. These properties make it
especially useful for aquatic mammals that live in cold water, such as whales
and seals.
Adipose tissue also forms a protective layer around some of the body organs, for
example the kidneys.

In plants, triglycerides often make up a major part of the energy stores in seeds,
either in the cotyledons (e.g. in sunflower seeds) or in the endosperm (e.g. in
castor beans).
Phospholipids Revised
A phospholipid molecule is like a triglyceride in which one of the fatty acids is

replaced by a phosphate group (Figure 2.11).
Two fatty acid chains Glycerol
Phosphate group
Hydrophobic tails Hydrophilic head

(fatty acid chains) (phosphate group)
Figure 2.11 Phospholipid molecules

The fatty acid chains have no electrical charge and so are not attracted to the
dipoles of water molecules (see p. 23). They are said to be hydrophobic.
The phosphate group has an electrical charge and is attracted to water
molecules. It is hydrophilic.
In water, a group of phospholipid molecules therefore arranges itself into a
bilayer, with the hydrophilic heads facing outwards into the water and the
hydrophobic tails facing inwards, therefore avoiding contact with water
(Figure 2.12).
Figure 2.12 A phospholipid bilayer

This is the basic structure of a cell membrane. The functions of phospholipids in
membranes are described on p. 33.
Test for lipids Revised
Mix the substance to be tested with absolute ethanol. Decant the ethanol into
water. A milky emulsion indicates the presence of lipid.
Proteins
Proteins are large molecules made of long chains of amino acids.
Amino acids Revised
All amino acids have the same basic structure, with an amine group and a
carboxyl group attached to a central carbon atom (Figure 2.13). There are
twenty different types of amino acid, which differ in the atoms present in the R
group. In the simplest amino acid, glycine, the R group is a single hydrogen atom.

The R group can represent
R a range of different groups
H2N C COOH
Amine group H Carboxyl group
Figure 2.13 An amino acid

Two amino acids can link together by a condensation reaction to form a
dipeptide. The bond that links them is called a peptide bond, and water is
produced in the reaction (Figure 2.14).
R R R R
H2N C COOH H2N C COOH H2N C CO HN C COOH
H H Condensation H H
H2O reaction Peptide bond
Figure 2.14 Formation of a dipeptide
The dipeptide can be broken down in a hydrolysis reaction, which breaks the
peptide bond with the addition of a molecule of water (Figure 2.15).
R R R R
H2O + H2N C CO HN C COOH H2N C COOH + H2N C COOH
H H Hydrolysis H H
reaction
Figure 2.15 Breakdown of a dipeptide
Structure of protein molecules Revised
Amino acids can be linked together in any order to form a long chain called a
polypeptide. A polypeptide can form a protein molecule on its own, or it can
associate with other polypeptides to form a protein molecule.
The sequence of amino acids in a polypeptide or protein molecule is called its
primary structure (Figure 2.16). Note that the three letters in each box are the
first three letters of the amino acid, for example Val is valine and Leu is leucine.
Figure 2.16 The primary structure of a small part of a polypeptide

The chain of amino acids often folds or curls up on itself. For example, many
polypeptide chains coil into a regular 3D shape called an alpha helix. This is
held in shape by hydrogen bonds between amino acids at different places in
the chain. This regular shape is an example of secondary structure of a protein
(Figure 2.17). Another example is the beta-pleated strand.
An alpha helix A beta-pleated strand
N
Hydrogen bond Hδ+ Hydrogen
between amino Oδ− bonds hold
acids C the helix in
shape
Figure 2.17 Examples of secondary structure

The polypeptide chain can also fold around on itself to form a more complex
Primary structure is the sequence of
three-dimensional shape. This is called the tertiary structure of the protein.
amino acids in a polypeptide or protein.
Once again, hydrogen bonds between amino acids at different points in the
Secondary structure is the first level
chain help to hold it in its particular 3-D shape. There are also other bonds
of folding of the amino acid chain.
involved, including ionic bonds, disulfide bonds and hydrophobic
interactions (Figure 2.18). Tertiary structure is the second level
of folding of the chain.
Quaternary structure is the
The globular shape of this association of two or more polypeptide
polypeptide is an example chains.
of tertiary structure
Figure 2.18 Tertiary structure of a protein

Many proteins are made of more than one polypeptide chain. These chains are
held together by the same type of bonds as in the tertiary structure (Figure 2.19).
The overall structure of the molecule is known as the quaternary structure of
the protein. The tertiary and quaternary structures of a protein, and therefore its
properties, are ultimately determined by its primary structure.
Hydrogen Ionic Disulfide bond Hydrophobic
bond bond (a covalent bond) interaction
C C
C
N
CH3 CH2
C
Hδ+ CH3
O− O S
S CH3
Oδ− NH3+ CH2 CH2
C C C C
Figure 2.19 Bonds involved in maintaining the secondary, tertiary and quaternary
structure of proteins
Globular and fibrous proteins Revised
Globular proteins have molecules that fold into a roughly spherical three-
dimensional shape. Examples include haemoglobin, insulin and enzymes. They
are often soluble in water and may be physiologically active — that is, they are
involved in metabolic reactions within or outside cells.
Fibrous proteins have molecules that do not curl up into a ball. They have
long, thin molecules, which often lie side by side to form fibres. Examples
include keratin (in hair) and collagen (in skin and bone). They are not soluble
in water and are not generally physiologically active. They often have structural
roles.
Haemoglobin — a globular protein

Figure 2.20 shows the structure of a haemoglobin molecule.
α polypeptide Haem group

chain Fe2+
Fe
2+
(oxygen binding site)
β polypeptide
chain
β polypeptide
chain
Fe2+
Fe
2+
α polypeptide
chain
Figure 2.20 The structure of haemoglobin

Relationship between structure and function in haemoglobin
The function of haemoglobin is the transport of oxygen from the lungs to Typical mistake
respiring tissues. It is found inside red blood cells. Although it may sound unlikely, it
l Solubility The tertiary structure of haemoglobin makes it soluble. The four is common for students to confuse
polypeptide chains are coiled up so that R groups with small charges on haemoglobin molecules with red
them (hydrophilic groups) are on the outside of the molecule. They therefore blood cells. Remember that each red
cell contains millions of haemoglobin
form hydrogen bonds with water molecules. Hydrophobic R groups are molecules.
mostly found inside the molecule.
l Ability to combine with oxygen The haem group contained within each
polypeptide chain enables the haemoglobin molecule to combine with
oxygen. Oxygen molecules combine with the iron ion, Fe2+, in the haem
group. One oxygen molecule (two oxygen atoms) can combine with each
haem group, so one haemoglobin molecule can combine with four oxygen
molecules (eight oxygen atoms).
l Pick-up and release of oxygen The overall shape of the haemoglobin
molecule enables it to pick up oxygen when the oxygen concentration is
high, and to release oxygen when the oxygen concentration is low. Small
changes in oxygen concentration have a large effect on how much oxygen
the haemoglobin molecule can hold. Once one oxygen molecule has
combined with one haem group, the whole molecule changes its shape
in such a way that it is easier for oxygen to combine with the other three
haem groups. (See also information about the oxygen dissociation curve for
haemoglobin on pp. 56–57.)
Collagen — a fibrous protein

Figure 2.21 shows the structure of collagen.
Polypeptide chain
Key
Gly glycine
Pro proline
Every third amino Hyp hydroxyproline
acid is glycine Lys lysine
Collagen is formed
from three polypeptide
chains held together
by hydrogen bonds
Figure 2.21 The structure of collagen
Relationship between structure and function in collagen

The function of collagen is to provide support and some elasticity in many
different animal tissues, such as human skin, bone and tendons.
l Insolubility Collagen molecules are very long and are too large to be able to
dissolve in water.
l High tensile strength Three polypeptide chains wind around one another,
held together by hydrogen bonds, to form a three-stranded molecule that
can withstand quite high pulling forces without breaking. This structure also
allows the molecules to stretch slightly when pulled.
l Compactness Every third amino acid in each polypeptide is glycine, whose
R group is just a single hydrogen molecule. Their small size allows the three
polypeptide chains in a molecule to pack very tightly together.
l Formation of fibres There are many lysine molecules in each polypeptide,
facing outwards from the three-stranded molecule. This allows covalent
bonds to form between the lysine R groups of different collagen molecules,
causing them to associate to form fibres.

Test for proteins
Revised
Add biuret solution. A purple colour indicates the presence of protein.
4 Explain the difference between each of the following:

a the secondary structure and the tertiary structure of a protein
b a collagen molecule and a collagen fibre
Answer on p.202
Revision activities
Make a list of all the polymers described on pp. 16–22. Then construct and
complete a table with the following headings:
l Polymer
l Monomers from which it is made
l Bonds linking the monomers
l Functions
Construct and complete a table with the following headings:
l Biological molecule
l How to test for it
l Positive results
Water
About 80% of the body of an organism is water. Water has unusual properties
compared with other substances, because of the structure of its molecules. Each
water molecule has a small negative charge (δ−) on the oxygen atom and a small
positive charge (δ+) on each of the hydrogen atoms. This is called a dipole.
There is an attraction between the δ− and δ+ parts of neighbouring water
molecules. This is called a hydrogen bond (Figure 2.22).
A single water molecule Hydrogen bonding between O

water molecules
δ− H H
O
O
H
δ+ δ+
H H
H
Hydrogen bond
H
Figure 2.22 Water molecules
Solvent properties of water Revised
The dipoles on water molecules make water an excellent solvent. For example, if
you stir sodium chloride into water, the sodium and chloride ions separate and
spread between the water molecules — they dissolve in the water. This happens
because the positive charge on each sodium ion is attracted to the small
negative charge on the oxygen of the water molecules. Similarly, the negative
chloride ions are attracted to the small positive charge on the hydrogens of the
water molecules.

Any substance that has fairly small molecules with charges on them, or that can
separate into ions, can dissolve in water (Figure 2.23).

Chloride ion Water
Sodium ion molecule
Sodium chloride crystal Chloride ion in solution Sodium ion in solution
Hydrogen
bond
Glucose crystal Glucose in solution
Figure 2.23 Water as a solvent

Because it is a good solvent, water helps to transport substances around the
bodies of organisms. For example, the blood plasma of mammals is mostly
water, and carries many substances in solution, including glucose, oxygen and
ions such as sodium. Water also acts as a medium in which metabolic reactions
can take place, as the reactants are able to dissolve in it.
Thermal properties of water Revised

l Water is liquid at normal Earth temperatures. The hydrogen bonds
between water molecules prevent them flying apart from each other at
normal temperatures on Earth. Between 0 °C and 100 °C, water is in the
liquid state. The water molecules move randomly, forming transitory
hydrogen bonds with each other. Other substances whose molecules
have a similar structure, such as hydrogen sulfide (H2S), are gases at these
temperatures, because there are no hydrogen bonds to attract their
molecules to each other.
l Water has a high latent heat of evaporation. When a liquid is heated, its
molecules gain kinetic energy, moving faster. Those molecules with the most
energy are able to escape from the surface and fly off into the air. A great
deal of heat energy has to be added to water molecules before they can do
this, because the hydrogen bonds between them have to be broken. When
water evaporates, it therefore absorbs a lot of heat from its surroundings.
The evaporation of water from the skin of mammals when they sweat
therefore has a cooling effect. Transpiration from plant leaves is important in
keeping them cool in hot climates.
l Water has a high specific heat capacity. Specific heat capacity is the
amount of heat energy that has to be added to a given mass of a substance
Now test yourself
to raise its temperature by 1 °C. Temperature is related to the kinetic energy 5 a Explain what a hydrogen bond
of the molecules — the higher their kinetic energy, the higher the is.
temperature. A lot of heat energy has to be added to water to raise its b Describe how hydrogen bonds
temperature, because much of the heat energy is used to break the affect the properties of water.
hydrogen bonds between water molecules, not just to increase their speed c State two types of
of movement. This means that bodies of water, such as oceans or a lake, do macromolecule that contain
not change their temperature as easily as air does. It also means that the hydrogen bonds.
bodies of organisms, which contain large amounts of water, do not change Answer on p.202
temperature easily. Tested

3 Enzymes
Enzyme mode of action

An enzyme is a globular protein that acts as a biological catalyst — that is, it
speeds up a metabolic reaction without itself being permanently changed. Some
enzymes work inside cells, and are called intracellular enzymes. Some work
outside cells (for example, inside the human alimentary canal) and are called
extracellular enzymes.
The substance present at the start of an enzyme-catalysed reaction is called the
substrate. The product is the new substance or substances formed.
Active sites Revised
In one part of the enzyme molecule, there is an area called the active site,
where the substrate molecule can bind. This produces an enzyme–substrate Typical mistake
complex. The 3-D shape of the active site fits the substrate perfectly, so only one Remember that it is the enzyme that
type of substrate can bind with the enzyme. The enzyme is therefore specific has an active site, not the substrate.
for that substrate. The enzyme can be considered to be a lock, and the substrate
the key that fits into the lock. However, in most cases the substrate actually
causes a small change in the shape of the active site, allowing the two to fit
Typical mistake
together. This is called the induced-fit hypothesis.
Do not say that the enzyme’s
The R groups of the amino acids at the active site are able to form temporary active site and the substrate have
bonds with the substrate molecule. This pulls the substrate molecule slightly out the ‘same’ shape. Their shapes are
of shape, causing it to react and form products. complementary.
Activation energy Revised
Substrates generally need to be supplied with energy to cause them to change

into products. The energy required to do this is called activation energy. In a
laboratory, you might supply energy by heating to cause two substances to react
together.
Enzymes are able to make substances react even at low temperatures. They
reduce the activation energy needed to make the reaction take place. They
do this by distorting the shape of the substrate molecule when it binds at the
enzyme’s active site.
Enzymes 25

3 Enzymes

How to… Follow the course of an enzyme-catalysed reaction
You can follow what happens over time in a reaction catalysed You could measure the rate of disappearance of starch in the
by an enzyme by: reaction:
l measuring the rate of formation of the product amylase
starch maltose
l measuring the rate of disappearance of the substrate
Add amylase solution to starch suspension in a test tube. Take
For example, you can measure the rate of formation of oxygen
samples of the reacting mixture at regular time intervals, and
in this reaction:
test for the presence of starch using iodine in potassium iodide
catalase
hydrogen peroxide oxygen + water solution. If starch is still present, you will obtain a blue-black
All biological material contains catalase. You could mash up colour. If there is no starch present, the iodine solution will
some potato tuber or celery stalks, mix them with water and remain orange-brown.
filter the mixture to obtain a solution containing catalase. This To obtain quantitative results, you could use a colorimeter. Put
can then be added to hydrogen peroxide in a test tube. Use some of the iodine solution into one of the colorimeter tubes,
relatively small tubes, so that there is not too much gas in the place it in the colorimeter and adjust the dial to give a reading
tube above the liquid. of 0. This is your standard, with no starch. Every minute, take
You could measure the rate of oxygen formation by collecting a sample of the liquid from the starch–amylase mixture and
the gas in a gas syringe and recording the volume every minute add it to a clean colorimeter tube containing iodine solution.
until the reaction stops (Figure 3.1). Note: don’t worry if you Mix thoroughly, then measure the absorbance (Figure 3.2). The
don’t have gas syringes. You could collect the oxygen in an darker the blue-black colour, the greater the absorbance, and
inverted measuring cylinder over water instead. the greater the concentration of starch.
Total volume 50
Gas syringe of oxygen
collected/cm3
40
30
20
Reacting mixture
10
0
0 20 40 60
Time/s
Figure 3.1 Following the time course of the breakdown of hydrogen peroxide by catalase
Light 0.8
absorbance/
optical density
0.6
0.4
0.2
0
0 5 10 15
Time/min
Figure 3.2 Following the time course of the breakdown of starch by amylase

Factors affecting the rate
3 Enzymes
of enzyme-catalysed
reactions
When an enzyme solution is added to a solution of its substrate, the random
movements of enzyme and substrate molecules cause them to collide with
each other.
As time passes, the quantity of substrate decreases, because it is being changed
into product. This decrease in substrate concentration means that the frequency
of collisions between enzyme and substrate molecules decreases, so the rate of
the reaction gradually slows down. The reaction rate is fastest right at the start
of the reaction, when substrate concentration is greatest.
When comparing reaction rates of an enzyme in different circumstances, we
should therefore try to measure the initial rate of reaction — that is, the rate of
reaction close to the start of the reaction.
Temperature Revised
At low temperatures, enzyme and substrate molecules have little kinetic energy.
They move slowly, and so collide infrequently. This means that the rate of
reaction is low. If the temperature is increased, then the kinetic energy of the
molecules increases. Collision frequency therefore increases, causing an increase
in the rate of reaction.
Above a certain temperature, however, hydrogen bonds holding the enzyme
molecule in shape begin to break. This causes the tertiary structure of the
enzyme to change, an effect called denaturation. This affects the shape of its
active site. It becomes less likely that the substrate molecule will be able to bind
with the enzyme, and the rate of reaction slows down.
The temperature at which an enzyme works most rapidly, just below that at
which denaturation begins, is called its optimum temperature (Figure 3.3).
Enzymes in the human body generally have an optimum temperature of about
37 °C, but enzymes from organisms that have evolved to live in much higher or
lower temperatures may have much higher or lower optimum temperatures.
Rate of At the enzyme’s

reaction optimum temperature
the rate of reaction
As the temperature rises, is maximal.
the extra energy in the
reactants results in faster
rates of reaction. The rate
is approximately doubled
for a 10 degree rise. As the temperature
rises above optimum,
the enzyme is
increasingly denatured.
0 20 40 60
Temperature/ºC
Figure 3.3 How temperature affects the rate of an enzyme-catalysed reaction
Enzymes 27

3 Enzymes

How to… Investigate the effect of temperature on enzyme activity
You can use almost any enzyme reaction for this, such as the Take the first flask, dry its base and sides and stand it on a
action of catalase on hydrogen peroxide, as described on p. 26. sensitive top-pan balance. Pour in the solution containing
You could use the same method of collecting the gas that is catalase (see p. 26) that is at the same temperature, and
described there, but here is another possible method. immediately take the balance reading. Record the new balance
Set up several small conical flasks containing the same volume readings every 30 seconds (or even more frequently if you can
of hydrogen peroxide solution of the same concentration. manage it) for about 3 minutes. The readings will go down as
Stand each one in a water bath at a particular temperature. Use oxygen is given off. This represents the mass of product formed.
at least five different temperatures over a good range — say Repeat with the solutions kept at each of the other
between 0 °C and 90 °C. (If time allows, set up three sets of temperatures.
tubes at each temperature. You will then be able to calculate Work out the initial rate of each reaction, either taken directly
the mean result for each temperature, which will give you a from your readings, or by drawing a graph of mass lost (which
more representative finding.) is the mass of oxygen) against time for each temperature, and
Take a set of test tubes and add the same volume of catalase then working out the gradient of the graph over the first
solution to each one. Stand these in the same set of water 30 seconds or 60 seconds of the reaction (Figure 3.4).
baths. Now you can use your results to plot a graph of initial rate of
Leave all the flasks and tubes to come to the correct reaction (y-axis) against temperature.
temperature. Check with a thermometer.
Amount of
product (P )
1 Draw a line from the origin through 2 Calculate the gradient

the early part of the graph. Include P1 of the line you have drawn.
the part of the line where it is straight. P
It will only be straight for a short time Rate = 1
T1
after the start of the reaction.
T1
Time (T )
Figure 3.4 Finding the initial rate of reaction
pH Revised
pH affects ionic bonds that hold protein molecules in shape. Because enzymes
are proteins, their molecules are affected by changes in pH. Most enzyme
molecules only maintain their correct tertiary structure within a very narrow pH
range (Figure 3.5), generally around pH 7. Some, however, require a very different
pH; one example is the protein-digesting enzyme pepsin found in the human
stomach, which has an optimum pH of 2.
Rate of
reaction
At pH values either side of the

optimum, the bonds holding
the enzyme’s tertiary shape,
including that of the active site,
are disrupted. If the shape of
the active site is changed, the
enzyme loses some of its activity. At extreme pHs the
enzyme is denatured
and inactivated.
Optimum pH pH
Figure 3.5 How pH affects the rate of an enzyme-catalysed reaction

3 Enzymes
How to… Investigate the effect of pH on enzyme activity
You can adapt the method described in How to... on p. 28 if acidic or alkaline products are formed.) Keep temperature,
to investigate the effect of pH on the rate of breakdown of enzyme concentration, substrate concentration and total
hydrogen peroxide by catalase. volume of reactants the same for all the tubes. Record, process
Vary pH by using different buffer solutions added to each and display results as before.
enzyme solution. (A buffer solution keeps a constant pH, even
Enzyme concentration Revised
The greater the concentration of enzyme, the more frequent the collisions
between enzyme and substrate, and therefore the faster the rate of the reaction.
However, at very high enzyme concentrations, the concentration of substrate
may become a limiting factor, so the rate does not continue to increase if the
enzyme concentration is increased (Figure 3.6).
Rate of
reaction When there are more enzyme
molecules than substrate molecules,
increasing the enzyme concentration
When there are more substrate does not result in a higher rate of
molecules than enzyme molecules, reaction as there are no spare
increasing the enzyme concentration substrate molecules for the enzyme
produces a higher rate of reaction. to act on.
The rate of reaction is in proportion
to the concentration of enzyme used.
Enzyme concentration
Figure 3.6 How enzyme concentration affects the rate of an enzyme-catalysed
reaction

How to… Investigate the effect of enzyme concentration on rate of reaction
You could use the following method to investigate the effect of volume of hydrogen peroxide solution at the next step. Ensure
enzyme concentration on the rate at which the enzyme catalase that each tube is labelled with a waterproof marker. If time and
converts its substrate, hydrogen peroxide, to water and oxygen. materials allow, prepare three sets of these solutions.
Prepare a catalase solution as described on p. 26. Place each tube in a water bath at 30 °C.
Prepare different dilutions of this solution (Table 3.1). Take another set of tubes and add 10 cm3 of hydrogen peroxide
solution to each one. The concentration of hydrogen peroxide
Table 3.1
must be the same in each tube. Stand these tubes in the same
Relative water bath.
concentration Leave all the tubes for at least 5 minutes to allow them to come
Volume of of catalase (as a to the correct temperature. When ready, add the contents of
Volume distilled percentage of the one of the hydrogen peroxide tubes to the first enzyme tube.
of initial water added/ concentration of Mix thoroughly. Measure the volume of gas collected in the gas
solution/cm3 cm3 the initial solution)
syringe after 2 minutes. If you are using three sets, then repeat
10 0 100 using the other two tubes containing the same concentration
9 1 90 of enzyme.
8 2 80 Do the same for each of the tubes of enzyme. Record the
mean volume of gas produced in 2 minutes for each enzyme
Continue until the final ‘solution’ prepared is 10 cm3 of distilled concentration and plot a line graph to display your results.
water. Note: if you find that you get measurable volumes of gas sooner
Place each solution into a tube fitted with a gas syringe (see than 2 minutes after mixing the enzyme and substrate, then
p. 26). Use relatively small tubes, so that there is not too much take your readings earlier. The closer to the start of the reaction
gas in the tube above the liquid, but leave space to add an equal you make the measurements, the better.
Enzymes 29

Substrate concentration
3 Enzymes
Revised
The greater the concentration of substrate, the more frequent the collisions
between enzyme and substrate, and therefore the faster the rate of the reaction.
However, at high substrate concentrations, the concentration of enzyme may
become a limiting factor, so the rate does not continue to increase if the Vmax is the maximum rate of activity
substrate concentration is increased (Figure 3.7). The maximum rate that is of the enzyme before substrate
reached is known as Vmax . concentration becomes limiting.
The Michaelis–Menten constant,
The Michaelis–Menten constant, Km, can be used to compare the affinity of
Km, is the substrate concentration at
different enzymes for their substrates. Km is the substrate concentration at which the rate of enzyme activity is
which the rate of activity of the enzyme is ½Vmax. The lower the value of Km, the ½Vmax.
greater the affinity of the enzyme for its substrate.
Rate of Vmax When there is a high

reaction concentration of
substrate molecules,
When there is a low each enzyme
concentration of molecule is working
substrate molecules, as fast as it can.
repeating the ½Vmax Repeating the
experiment with experiment with more
more substrate substrate does not
produces a higher
rate of reaction. The
result in a higher rate
of reaction, as
Now test yourself
rate of reaction is in enzyme molecules
1 Outline how you could compare
proportion to the cannot bind substrate
concentration of and change it to
the affinity of catalase from two
Km different fruits for their substrate.
substrate. Substrate concentration product any faster.
Answer on pp.202–203
Figure 3.7 How substrate concentration affects the rate of an enzyme-catalysed Tested
reaction
How to… Investigate the effect of substrate concentration on the rate of an enzyme catalysed reaction
You can do this in the same way as described for investigating concentration of catalase the same and vary the concentration
the effect of enzyme concentration, but this time keep the of hydrogen peroxide.
Inhibitors Revised
An inhibitor is a substance that slows down the rate at which an enzyme

works (Figure 3.8). Many inhibitors are reversible — that is, they do not attach
permanently to the enzyme.
Competitive inhibitors generally have a similar shape to the enzyme’s normal
substrate. They can fit into the enzyme’s active site, preventing the substrate
from binding. The greater the proportion of inhibitor to substrate in the mixture,
the more likely it is that an inhibitor molecule, and not a substrate molecule,
will bump into an active site. The degree to which a competitive inhibitor slows
down a reaction is therefore affected by the relative concentrations of the
inhibitor and the substrate.
Non-competitive inhibitors do not have the same shape as the substrate, and
they do not bind to the active site. They bind to a different part of the enzyme.
This changes the enzyme’s shape, including the shape of the active site, so the
substrate can no longer bind with it. Even if you add more substrate, it still will
not be able to bind, so the degree to which a non-competitive inhibitor slows
down a reaction is not affected by the relative concentrations of the inhibitor
and the substrate.

3 Enzymes
Enzyme acting on its normal substrate
Enzyme
Active site
Enzyme-substrate complex
Substrate
Products
Enzyme in the presence of a competitive inhibitor
With the competitive inhibitor Now test yourself

bound at the active site,
the normal substrate 2 Temperature, pH, enzyme
cannot bind. concentration, substrate
Competitive concentration and inhibitors can all
inhibitor affect the rate of enzyme activity.
a Which of these have their effect
Enzyme in the presence of a non-competitive inhibitor by changing the frequency of
collisions between the substrate
Non-competitive With the non-competitive and the enzyme’s active site?
inhibitor inhibitor bound to the b Which have their effect by
enzyme, the active site is
changing the shape of the active
changed and the normal
site?
substrate cannot bind.
Answer on p.203
Tested
Figure 3.8 Competitive and non-competitive enzyme inhibitors
Immobilising enzymes Revised
Enzymes are used as catalysts in many industrial processes. The enzymes are
often immobilised by fixing them onto or inside a gel or other substance, which
prevents the enzymes from dissolving in the reacting mixture. This means that
the product is not contaminated with dissolved enzyme, and the enzymes can
be repeatedly reused. Immobilisation also enables enzymes to work over a wider
range of temperature and pH than when they are in free solution, probably
because the trapped enzyme molecules cannot easily change shape and become
denatured. One way of immobilising enzymes is to trap them inside little balls of
calcium alginate.

How to… Investigate the effect of immobilisation on the rate of an enzyme-catalysed reaction
You can immobilise enzymes inside calcium alginate balls. First, solution. The calcium chloride and sodium alginate will react to
make up a solution of 1.5% calcium chloride, and another of form little beads of jelly-like calcium alginate, with the enzyme
2% sodium alginate. Mix the chosen enzyme solution into trapped in them. You can now compare the rate of activity
the sodium alginate solution. Using a pipette, draw up a small of the enzyme when trapped in the beads, and when free in
volume of the mixture, and drop it into the calcium chloride solution.
3 List the reasons why immobilised enzymes, rather than enzymes in solution, are often used in industrial processes.
Answer on p.203
Enzymes 31

Fluid mosaic membranes

Every cell is surrounded by a cell membrane. There are also many membranes
within cells. The membrane around the outside of a cell is called the cell surface
membrane.
Structure of a cell membrane Revised
A cell membrane consists of a double layer of phospholipid molecules

(p. 19). This structure arises because in water a group of phospholipid molecules
arranges itself into a bilayer, with the hydrophilic heads facing outwards into the
water and the hydrophobic tails facing inwards, therefore avoiding contact with
water.
This is the basic structure of a cell membrane. There are also cholesterol
molecules among the phospholipids. Protein molecules float in the
phospholipid bilayer. Many of the phospholipids and proteins have short chains
of carbohydrates attached to them, on the outer surface of the membrane.
They are known as glycolipids and glycoproteins. There are also other types of
glycolipid with no phosphate groups.
This is called the fluid mosaic model of membrane structure (Figure 4.1):
l ‘Fluid’ because the molecules within the membrane can move around within
their own layers.
l ‘Mosaic’ because the protein molecules are dotted around within the
membrane.
l ‘Model’ because no-one has ever seen a membrane looking like the
diagram — the molecules are too small to see even with the most powerful
microscope. The structure has been worked out because it explains the
behaviour of membranes that has been discovered through experiment.
The roles of the components of cell membranes are outlined in Table 4.1.
Chain of
Glycolipid
sugars
Glycoprotein
Chain of Lipid
sugars
Protein
Phospholipid
bilayer
Phospholipid Channel protein Cholesterol
Figure 4.1 The fluid mosaic model of membrane structure

Table 4.1 Cell membrane components and their roles

Component Roles
Phospholipids Form the fluid bilayer that is the fundamental structure of the
membrane.
Prevent hydrophilic substances — such as ions and some
molecules — from passing through.
Cholesterol Helps to keep the cell membrane fluid.
Proteins and Provide channels that allow hydrophilic substances to pass
glycoproteins through the membrane; these channels can be opened or
closed to control the substances’ movement.
Actively transport substances through the membrane against
their concentration gradient, using energy derived from ATP.
Act as receptor molecules for substances such as hormones,
which bind with them; this can then affect the activity of the
cell.
Cell recognition — cells from a particular individual or
a particular tissue have their own set of proteins and
glycoproteins on their outer surfaces.
Glycolipids Cell recognition and adhesion to neighbouring cells to form
tissues.
Cell signalling Revised
Cells can communicate with one another using molecules that interact with
cell membranes. Cell surface membranes contain receptor molecules, into
which signalling molecules can fit. Like enzymes, these receptors are specific,
only accepting one type of signalling molecule. This means that the signalling
molecule can only affect cells that have its receptor in their cell surface
membranes. These are its target cells.
For example, the hormone insulin is a protein that fits into receptors in the
cell surface membrane of liver cells. When insulin is bound to the receptor, this
brings about changes in the cell that result in an increase of transporter proteins
for glucose in the cell surface membrane, causing the cell to take up glucose.
Movement of substances
into and out of cells
Passive transport through cell membranes Revised
Molecules and ions are in constant motion. In gases and liquids they move
freely. As a result of their random motion, each type of molecule or ion tends to
Diffusion is the net movement of
spread out evenly within the space available. This is diffusion. Diffusion results molecules or ions down a concentration
in the net movement of ions and molecules from a high concentration to a low gradient, as a result of the random
concentration. movement of particles.
Diffusion across a cell membrane

Some molecules and ions are able to pass through cell membranes. The
membrane is permeable to these substances. However, some substances cannot
pass through cell membranes, so the membranes are said to be partially
permeable.
Cell membranes and transport 33

For example, oxygen is often at a higher concentration outside a cell than inside,
because the oxygen inside the cell is being used up in respiration. The random
motion of oxygen molecules inside and outside the cell means that some of
them ‘hit’ the cell surface membrane. More of them hit the membrane on
the outside than the inside, because there are more of them outside. Oxygen
molecules are small and do not carry an electrical charge, so they are able to
pass freely through the phospholipid bilayer. Oxygen therefore diffuses from
outside the cell, through the membrane, to the inside of the cell, down its
concentration gradient.
This is passive transport, because the cell does not do anything to cause the
oxygen to move across the cell membrane.
How to… Investigate the effect of surface area on diffusion rate
In general, large objects have a smaller surface area:volume ratio and sizes of jelly to cut, thinking about keeping the volume of
than small objects. You can use blocks of agar jelly to investigate each piece constant, but varying the surface area. Immerse your
how the rate of diffusion is affected by surface area:volume pieces of jelly in a dilute alkali (e.g. sodium hydrogencarbonate
ratio. The agar jelly can be made up using a little universal solution). As the alkali diffuses into the jelly, the indicator will
indicator solution. If the water used to dissolve the agar is change colour. You can time how long it takes for the whole
slightly acidic, then the jelly will be red. Decide on the shapes piece of jelly to change colour.
Facilitated diffusion
Ions or electrically charged molecules are not able to diffuse through the
phospholipid bilayer because they are repelled from the hydrophobic tails. Large
molecules are also unable to move through the phospholipid bilayer freely.
However, the cell membrane contains special protein molecules that provide Facilitated diffusion is the net
movement of molecules or ions through
hydrophilic passageways through which these ions and molecules can pass. They
channel proteins in a cell membrane,
are called channel proteins. Different channel proteins allow the passage of
down a concentration gradient, as a
different types of molecules and ions. Diffusion through these channel proteins result of the random movement of
is called facilitated diffusion. Like ‘ordinary’ diffusion, it is entirely passive particles.
(Figure 4.2).
Oxygen molecules Glucose molecules can diffuse
can diffuse through through a glucose channel protein
the bilayer
Now test yourself

1 Explain the difference between
diffusion and facilitated diffusion.
Answer on p.203
Channel protein
Tested
Figure 4.2 Diffusion across a cell membrane
Osmosis
Water molecules are small. They carry tiny electrical charges (dipoles) but
their small size means that they are still able to move quite freely through the
phospholipid bilayer of most cell membranes. Water molecules therefore tend to
diffuse down their concentration gradient across cell membranes.
Cell membranes always have a watery solution on each side. These solutions
may have different concentrations of solutes.
The greater the concentration of solute, the less water is present. The water
molecules in a concentrated solution are also less free to move, because they

are attracted to the solute molecules (see Figure 4.3). A concentrated solution is

therefore said to have a low water potential.
In a dilute solution, there are more water molecules and they can move more
freely. This solution has a high water potential.
Imagine a cell membrane with a dilute solution on one side and a concentrated
solution on the other side. The solute has molecules that are too large to get
through the membrane — only the water molecules can get through. The
membrane is said to be partially permeable.
Water molecules in the dilute solution are moving more freely and therefore hit
Osmosis is the diffusion of water
the membrane more often than water molecules in the concentrated solution.
molecules from a dilute solution to
More water molecules therefore diffuse across the membrane from the dilute to
a concentrated solution through a
the concentrated solution than in the other direction. The net movement of partially permeable membrane, down a
water molecules is from a high water potential to a low water potential, down a water potential gradient.
water potential gradient. This is osmosis (Figure 4.3).
Water The membrane allows water

molecules molecules to diffuse through
The solute
cannot pass
through the
membrane
Water molecules
are attracted to
the solute
Figure 4.3 Osmosis across a cell membrane
How to… Use Visking tubing to investigate osmosis
You can use an artificial partially permeable membrane called

Visking tubing to investigate osmosis. The apparatus in Figure
9 10 11 12 13 14 15
4.4 could be used to investigate how temperature or different Narrow Ruler

concentrations of solutions (differences in water potential) glass tube
affect the rate of osmosis.
Your results will be recorded as the level reached by the liquid
in the narrow glass tube at regular time intervals
8
7
6
5
4
3
2
1
0
Tube held tightly

Water by thread
Visking tubing
Concentrated sugar
solution
Figure 4.4 Using Visking tubing to investigate osmosis

Water potential is measured in pressure units, kilopascals (kPa). Pure water has a
water potential of 0 kPa. Solutions have negative water potentials. For example, a
dilute sucrose solution might have a water potential of −250 kPa. A concentrated
sucrose solution might have a water potential of −4000 kPa. The more negative
the number, the lower the water potential. Water moves by osmosis down a
water potential gradient, from a high (less negative) water potential to a low
(more negative) water potential (Figure 4.5).
–350 kPa –1050 kPa Net water movement occurs in

the direction of the arrows, from
cells with high water potential to
–3000 kPa cells with low water potential
Figure 4.5 Water movement between cells
Effects of osmosis on animal and plant cells

An animal cell placed in pure water takes up water by osmosis, as water
molecules diffuse down the water potential gradient into the cell. The volume of
the cell increases, and the cell may burst. Animals and protoctists (single-celled
organisms with animal-type cells) that live in fresh water have some way of
removing excess water from their cells, so that the cells do not swell and burst.
An animal cell in a concentrated solution loses water by osmosis, and will shrink.
A plant cell in pure water also takes up water by osmosis. However, it has a
strong cell wall outside its cell surface membrane, which prevents the cell from
bursting. Instead, the cell simply becomes swollen and is said to be turgid.
Turgidity helps plant tissues to remain in shape. For example, a plant leaf remains
in shape because of the turgidity of its cells.
A plant cell in a concentrated solution loses water by osmosis, so that the
volume of the cytoplasm and vacuole decrease. The cell loses its turgidity. Leaves
in which the cells lose their turgidity are no longer supported, and they wilt.
If a great deal of water is lost from a plant cell, then the cell contents shrink so
much that the cell surface membrane pulls away from the cell wall. This is called
plasmolysis (Figure 4.6). The membrane often remains attached at points where
plasmodesmata link to the next cell.
Cell wall Cell surface membrane External solution has

passed through the
cell wall and is still
Cytoplasm in contact with the
protoplast
Vacuole Cytoplasm has shrunk

away from the cell
wall — the cell is
fully plasmolysed
Figure 4.6 A plasmolysed plant cell

How to… Investigate the effect on plant cells of immersion in solutions of different water potentials
There are several different ways in which this investigation could l Peel off the inner epidermis from one of the 1 cm2 pieces of
be done. They include the following: onion, and place it carefully onto the drop on a slide of the
l Cut cylinders or discs or strips of a solid and uniform plant same concentration solution in which it has been immersed.
tissue, such as a potato tuber, then measure either their Take care not to let it fold over. Press it gently into the liquid
lengths or masses before immersing them in the solutions. using a section lifter or other blunt tool. Carefully place a
Leave them long enough for them to come to equilibrium, coverslip over it, taking care not to trap air bubbles.
and then measure the length or mass of each piece again, l Repeat with each of the other drops of liquid. Leave all of the
and calculate the percentage change in the measurement. slides for at least 5 minutes to give any water movements by
Percentage change can then be plotted against the osmosis time to take place and equilibrium to be reached.
concentration of the solution. l Observe each slide under the microscope. Count the total
l Cut small pieces of single-cell-thick plant tissue, for example number of cells in the field of view and record this. Then
onion epidermis. Mount them in a drop of sugar solution count the number that are plasmolysed and record this. Then
on microscope slides, and count the percentage of cells move to another area of the slide and repeat until you have
plasmolysed, or score each cell you see according to how counted at least 50 cells. Repeat for each slide.
plasmolysed it is. l Calculate the percentage of cells that have plasmolysed in
For the plasmolysis investigation, you could use this method: each solution. Add this to your results chart.
l Peel off one of the thick layers from an onion bulb. Cut six l Plot percentage of cells that have plasmolysed (y-axis) against
approximately 1 cm2 pieces from it. Put each piece into a the concentration of the solution (x-axis). Join the points with
different liquid. One should be distilled water, then a range either straight lines drawn between points, or a best-fit curve.
l The point at which the line crosses the 50% plasmolysis
of sucrose solutions from about 0.1 mol dm−3 up to about
1.0 mol dm−3 . level tells you the concentration of the solution at which, on
l Take six clean microscope slides and label each with the average, cells were just beginning to plasmolyse. At this value,
concentration of solution you are going to place on it. Put a the concentration of the solution inside the onion cells was,
drop of the relevant solution on each one. on average, the same as the concentration of the sucrose
solution.
Active transport across cell membranes Revised
Cells are able to make some substances move across their membranes up their
concentration gradients. For example, if there are more potassium ions inside
Active transport is the movement
the cell than outside the cell, the potassium ions would diffuse out of the cell.
of molecules or ions across a cell
However, the cell may require potassium ions. It may therefore use a process membrane against their concentration
called active transport to move potassium ions from outside the cell to inside gradient, using energy from respiration.
the cell, against the direction in which they would naturally diffuse.
This is done using carrier (transporter) proteins in the cell membrane. These use Now test yourself
energy from the breakdown of ATP to move the ions into the cell. The carrier
proteins are ATPases (Figure 4.7). 2 Explain the difference between
ATPase facilitated diffusion and active
ATP ADP + phosphate + energy transport.
Each carrier protein is specific to just one type of ion or molecule. Cells contain Answer on p.203
many different carrier proteins in their membranes. Tested
Glucose molecule
Cell membrane 1 A glucose molecule enters

the carrier protein.
Carrier protein
2 The carrier protein changes

shape. The energy needed
to do this comes from ATP.
3 The change of shape of

the carrier protein pushes
the glucose molecule into
the cell.
Figure 4.7 Active transport

Endocytosis and exocytosis
Revised
Cells can move substances into and out of the cell without the substances
having to pass through the cell membrane.
In endocytosis the cell puts out extensions around the object to be engulfed.
The membrane fuses together around the object, forming a vesicle.
In exocytosis the object is surrounded by a membrane inside the cell to form
a vesicle. The vesicle is then moved to the cell membrane. The membrane of
the vesicle fuses with the cell membrane, expelling its contents outside the cell
(Figure 4.8).
Exocytosis
Vesicle
Golgi
Nucleus apparatus
A vesicle is produced The vesicle moves to The membrane of the The contents of the
containing material to be the cell surface vesicle and the cell vesicle are released.
removed from the cell. membrane. surface membrane
join and fuse.
Endocytosis
The cell surface The object or solution The membrane breaks The vesicle moves
membrane grows out. is surrounded. and rejoins, enclosing inwards and its
the object. contents are absorbed.
Figure 4.8 Endocytosis and exocytosis
Table 4.2 summarises the methods of movement across membranes.
Table 4.2 Movement across cell membranes
Passive movement Active movement

Facilitated Endocytosis and
Feature Diffusion Osmosis diffusion Active transport exocytosis
Requires energy input No No No Yes Yes
from the cell
Involves the movement Yes Yes Yes Yes No
of individual ions or
molecules
Movement is through No No Yes Yes No
protein channels or
protein carriers in the
membrane
Examples of substances Oxygen, carbon Water Ions (e.g. K+, Cl−) Mostly ions (e.g. Droplets of liquid;
that move dioxide and molecules (e.g. K+, Cl−) bacteria; export
glucose) proteins

Division of cells
Chromosomes Revised
A chromosome is a molecule of DNA, associated with proteins called histones.

The DNA and histones are tightly coiled, so that the long DNA molecule packs
into a very small space.
Before cell division, each molecule of DNA is duplicated. The two identical
copies of tightly coiled DNA are called chromatids, and they remain attached
to one another at a point called a centromere (Figure 5.1).
DNA
Histones
Centromere
Nucleosome
Chromatids
Figure 5.1 Chromosome structure

The DNA in a chromosome contains coded instructions for making proteins in
the cell (see p. 44), called genes. When DNA is duplicated, it cannot be copied
right to the very end of the molecule. To avoid loss of genes, the ends of each
chromosome consists of telomeres, which are regions of non-coding DNA.
Each time the DNA is copied, a little of each telomere is lost, so it gradually
becomes shorter.
Mitosis Revised
A multicellular organism begins as a single cell. That cell divides repeatedly to

produce all the cells in the adult organism.
The type of cell division involved in growth is called mitosis. Mitosis is also used
to produce new cells to replace ones that have been damaged — that is, to
repair tissues. Mitosis is also involved in asexual reproduction, in which a single
parent gives rise to genetically identical offspring.
The mitotic cell cycle 39

Strictly speaking, mitosis is division of the nucleus of the cell. After this, the cell
itself usually divides as well. This is called cytokinesis.
The cell cycle Revised
During growth of an organism many of its cells go through a continuous cycle of

growth and mitotic division called the cell cycle (Figure 5.2).
S phase
(DNA replication)
The two G phases and S
phase make up interphase.
G1 phase G2 phase Typical mistake
Students often state that interphase or
cytokinesis are stages in mitosis. You
can see from Figure 5.2 that this is not
correct.
Cytokinesis Mitosis
Figure 5.2 The cell cycle
For most of the cell cycle, the cell continues with its normal activities. It also
grows, as the result of the production of new molecules of proteins and other
substances, which increase the quantity of cytoplasm in the cell.
DNA replication takes place during interphase, so that there are two identical
copies of each DNA molecule in the nucleus. (DNA replication is described
on p. 43–44.) Each original chromosome is made up of one DNA molecule, so
after replication is complete each chromosome is made of two identical DNA
molecules.
During mitosis, the two chromatids split apart and are moved to opposite ends
of the cell. A new nuclear envelope then forms around each group. These two
nuclei each contain a complete set of DNA molecules identical to those in the
original (parent) cell. Mitosis produces two genetically identical nuclei from one
parent nucleus (see Figure 5.3).
After mitosis is complete, the cell usually divides into two, with one of the
new nuclei in each of the two new cells. The two daughter cells are genetically
identical to each other and their parent cell.
Control of the cell cycle

Each cell contains genes that help to control when it divides. It is important that
cells divide by mitosis only when they are required to do so. This usually involves
signals from neighbouring cells, to which the cell responds by either dividing or
not dividing. If this control goes wrong, then cells may not divide when they
should (so growth does not take place, or wounds do not heal) or they may
divide when they should not (so that a tumour may form).

Mitosis
Prophase
● The chromosomes condense
● The centrioles duplicate
● The centriole pairs move towards
each pole
● The spindle begins to form
Metaphase
● The nuclear envelope disappears
● The centriole pairs are at the poles
● The spindle is completely formed
● The chromosomes continue to condense
● The spindle fibres attach to the
centromeres of the chromosomes

● The spindle fibres pull on the centromeres,
arranging them on the equator
Anaphase
● The links between sister chromatids break
● The centromeres of sister chromatids
move apart, pulled by the spindle fibres
Telophase
● Sister chromatids (now effectively separate
chromosomes) reach opposite poles

● The chromosomes decondense
● Nuclear envelopes begin to form around
the chromosomes at each pole

● The spindle disappears
Cytokinesis
● The cell divides into two cells, either by
infolding of the cell surface membrane in
animal cells, or by the formation of a new
cell wall and cell surface membrane in
plants
Figure 5.3 Mitosis and cytokinesis in an animal cell
Stem cells Revised
Animals begin their lives as a zygote, when a sperm and egg fuse together
at fertilisation. The zygote divides to form two, then four, then eight cells,
eventually forming a multicellular embryo. Each of the cells in this embryo
is potentially capable of dividing to form any of the many different types of
specialised cell in the animal’s body.
Cells that are able to divide to form specialised cells are called stem cells.
In an adult organism, most cells have already become specialised (they have
differentiated) and are not able to divide. However, most tissues contain some
stem cells that retain the ability to divide, and these are able to form new cells
for growth and tissue repair. In a young embryo, all cells are stem cells, and they
can form all types of specialised cell. They are said to be totipotent. In an adult,
most stem cells are only able to form a limited number of types of specialised
cell. For example, stem cells in the bone marrow are able to divide to form
different types of blood cell, but they cannot form nerve cells or muscle cells.
The mitotic cell cycle 41

6 Nucleic acids and protein
synthesis
DNA and RNA

Structure of nucleotides Revised
DNA and RNA are polynucleotides. Polynucleotides are substances whose

molecules are made of long chains of nucleotides linked together. A nucleotide
(Figure 6.1) is made up of:
l a 5-carbon sugar (deoxyribose in DNA; ribose in RNA) Typical mistake
l a phosphate group Take care with the spelling of adenine
and thymine – do not confuse them
l a nitrogen-containing base (adenine, guanine, cytosine or thymine in
with adenosine or thiamine.
DNA; adenine, guanine, cytosine or uracil in RNA)
The bases are usually referred to by their first letters, A, G, C, T and U.
A and G are purine bases, made up of two carbon–nitrogen rings. C, T and U
are pyrimidine bases, made up of one carbon–nitrogen ring.
Phosphate group
Organic base
Pentose sugar C (e.g. cytosine)
(e.g. deoxyribose)
Figure 6.1 A nucleotide
Nucleotides can link together by the formation of covalent bonds between the
phosphate group of one and the sugar of another (Figure 6.2). This takes place
through a condensation reaction.
Covalent bond
Figure 6.2 Part of a polynucleotide

An RNA molecule is usually made up of a single strand, although this may be
folded up on itself. A DNA molecule is made up of two strands, held together
by hydrogen bonds between the bases on the two strands. The strands run in
opposite directions, i.e. they are anti-parallel.
Hydrogen bonding only occurs between A and T and between C and G. This is
called complementary base pairing (Figure 6.3).

Hydrogen bonds
C G
Now test yourself

1 Use Figure 6.3 to explain why A can
A T only bond with T, not with another
A or with C or G.
Answer on p.203
Tested
T A
Figure 6.3 Part of a double-stranded DNA molecule Expert tip

Do not confuse bases (or nucleotides)
The two strands of nucleotides twist round each other to produce a double with amino acids.
helix.
The structure of ATP

Adenosine triphosphate, ATP, is a phosphorylated nucleotide, with a structure
similar to an RNA nucleotide (Figure 6.4).
NH2
N
O− O O− N
Adenine
O P O P O P O CH2 N N
− O
O O O
Ribose
Adenosine
Figure 6.4 An ATP molecule
DNA replication Revised
New DNA molecules need to be made before a cell can divide. The two
daughter cells must each receive a complete set of DNA. The base sequences on
the new DNA molecules must be identical with those on the original set. DNA
replication takes place in the nucleus, during interphase.
l Hydrogen bonds between the bases along part of the two strands are
broken. This ‘unzips’ part of the molecule, separating the two strands.
l Nucleotides that are present in solution in the nucleus are moving randomly
around. By chance, a free nucleotide will bump into a newly exposed one
with which it can form hydrogen bonds. Free nucleotides therefore pair up
with the nucleotides on each of the DNA strands, always A with T and C
with G (Figure 6.5).
l DNA polymerase links together the phosphate and deoxyribose groups of
adjacent nucleotides.
This is called semi-conservative replication, because each new DNA molecule
has one old strand and one new one.
Nucleic acids and protein synthesis 43

T A T T C G
A
G A T A A G C
C
C A T A T C T
A
G T A T A G
G T A T T C G
C
T A T A A G C
Figure 6.5 DNA replication
The genetic code

The sequence of bases in a DNA molecule is a code that determines the A gene is a sequence of nucleotides
sequence in which amino acids are linked together when making a protein that forms part of a DNA molecule, and
molecule. A sequence of DNA nucleotides that codes for one polypeptide, or for that codes for a polypeptide or protein.
one protein, is known as a gene.
As we have seen, the sequence of amino acids in a protein — its primary
structure — determines its three-dimensional shape and therefore its properties
and functions. For example, the primary structure of an enzyme determines the
shape of its active site, and therefore the substrate with which it can bind.
A series of three bases in a DNA molecule, called a base triplet, codes for
one amino acid. The DNA strand that is used in protein synthesis is called the
template strand. For example, Figure 6.6 shows the sequence of amino acids
coded for by the template strand of a particular length of DNA.
bases in DNA T A C C T G C A A C T T
amino acid in polypeptide methionine aspartate valine glutamate

Figure 6.6 A DNA strand and the amino acids it codes for
There are twenty amino acids. Because there are four bases, there are 43 = 64
different possible combinations of bases in a triplet. Some amino acids therefore
are coded for by more than one triplet. For example, the triplets AAA and AAG
both code for the amino acid phenylalanine. The code is therefore said to be
degenerate.
Protein synthesis Revised
Proteins are made on the ribosomes in the cytoplasm, by linking together amino
acids through peptide bonds. The sequence in which the amino acids are linked
is determined by the sequence of bases on a length of DNA in the nucleus.
Transcription
The first step in protein synthesis is for the sequence of bases on the template Typical mistake
strand of the DNA to be used to construct a strand of messenger RNA (mRNA) Students sometimes confuse
with a complementary sequence of bases. This is called transcription. transcription with DNA replication.
Read the question carefully and make
In the nucleus, the double helix of the DNA is unzipped, exposing the bases on sure you are writing about the correct
each strand. There are four types of free RNA nucleotide in the nucleus, with process.
the bases A, C, G and U. The RNA nucleotides form hydrogen bonds with the
exposed bases on the template strand of the DNA. They pair up as shown in
Table 6.1.

Table 6.1 DNA – RNA base pairing

Base on DNA strand Base on RNA strand
A U
C G
G C
T A
As the RNA nucleotides slot into place next to their complementary bases on
the DNA, the enzyme RNA polymerase links them together (through their
sugar and phosphate groups) to form a long chain of RNA nucleotides. This is an
mRNA molecule.
The mRNA molecule contains a complementary copy of the base sequence
on the template strand of part of a DNA molecule. Each triplet on the DNA is
represented by a complementary group of three bases on the mRNA, called a
codon (Figure 6.7).
1 Part of a molecule of DNA 2 The hydrogen bonds between
bases are broken, exposing
the bases
G
C T
C A T G G A C T G C A T G G A
G A G A G A G
T C C T C T C C T C
G T
C A T G G A C T G C A T G G A A
C
G T A C C T G A C G T A C C T G A
C
3 Free RNA nucleotides in the nucleus 4 The RNA nucleotides are linked
form new hydrogen bonds with the together to form an mRNA
T G
C template
exposed bases
G on
A the strand Cmolecule
A T G G A C T G
G
T G
A C T
A G C A T G G A C T G
C
C
G
G
T Now test yourself
C TA A
C
A
C
C U G C A U G G A C U G 2 How many amino acids are coded
G C for by the length of DNA in
T T
A G A C
A G T A C C T G A C
C
C U G C A U G G A C U G Figure 6.7?
C
T G A C G T A C C T G A C Answer on p.203
Tested
Figure 6.7 Transcription of part of a DNA molecule
Translation
The mRNA molecule breaks away from the DNA, and moves out of the nucleus
into the cytoplasm. It becomes attached to a ribosome. Two codons fit into a
groove in the ribosome. The first codon is generally AUG, which is known as a
start codon. It codes for the amino acid methionine.
In the cytoplasm, 20 different types of amino acid are present. There are also
many different types of transfer RNA (tRNA) molecule. Each tRNA molecule is
made up of a single strand of RNA nucleotides, twisted round on itself to form a
clover-leaf shape. There is a group of three exposed bases, called an anticodon.
There is also a position at which a particular amino acid can be loaded by a
specific enzyme (Figure 6.8).
The amino acid that can be loaded onto the tRNA is determined by the base
sequence of its anticodon. For example, a tRNA whose anticodon is UAC will be
loaded with the amino acid methionine.

Specific binding site
for amino acid
Now test yourself

3 What will the tRNA anticodons be,
to fit against the mRNA shown in
Figure 6.7?
Answer on p.203
Three bases forming the anticodon
Tested
Figure 6.8 A tRNA molecule
The amino acids carried by the two adjacent tRNAs are then linked by a peptide
bond.
The mRNA is then moved along one place in the ribosome, and a third tRNA
slots into place against the next mRNA codon. A third amino acid is added to
the chain.
This continues until a stop codon is reached on the mRNA. This is a codon that
does not code for an amino acid, such as UGA. The polypeptide (long chain of
amino acids) that has been formed breaks away.
This process of building a chain of amino acids following the code on an mRNA
molecule is called translation (Figure 6.9).
New polypeptide being formed Amino acid
tRNA
C U A
U C
A
A C G
A U G G A U U G C G A U U G C A C G
Start codon mRNA

Ribosome
Figure 6.9 Translation
Mutation Revised
A mutation is a random, unpredictable change in the DNA in a cell. It may be:

l a change in the sequence of bases in one part of a DNA molecule
l an addition of extra DNA to a chromosome or a loss of DNA from it
l a change in the total number of chromosomes in a cell
Mutations are most likely to occur during DNA replication, for example when
a ‘wrong’ base might slot into position in the new strand being built. Enzymes
repair almost all these mistakes immediately, but some can persist.

A change in the sequence of bases in DNA can result in a change in the

sequence of amino acids in a protein. (Note that this does not always happen,
because there is more than one triplet that codes for each amino acid, so a A gene mutation is a change in the
change in a triplet may not change the amino acid that is coded for.) This in sequence of nucleotides, which may
turn may result in a change in the 3-D structure of the protein and therefore the result in an altered polypeptide.
way that it behaves.
Sickle cell anaemia

An example of a mutation is a change in the gene that codes for one of the
polypeptides in a haemoglobin molecule. In the genetic disease sickle cell
anaemia, the gene that codes for the β polypeptide has the base T where it
should have the base A. This means that one triplet is different, so a different
amino acid is used when the polypeptide chain is constructed on a ribosome.
These two different forms of the gene are called alleles. The normal allele is
HbS, and the sickle cell allele is HbA.
Normal allele
DNA base sequence:
GTG CAC CTG ACT CCT GAG GAG AAG TCT
Amino acid sequence:
Val-His-Leu-Thr-Pro-Glu-Glu-Lys-Ser-
Abnormal allele
DNA base sequence:
GTG CAC CTG ACT CCT GTG GAG AAG TCT
Amino acid sequence:
Val-His-Leu-Thr-Pro-Val-Glu-Lys-Ser-
The abnormal β polypeptide has the amino acid valine where it should have
the amino acid glutamic acid. These amino acids are on the outside of the
haemoglobin molecule when it takes up its tertiary and quaternary shapes.
Glutamic acid is a hydrophilic amino acid. It interacts with water molecules,
helping to make the haemoglobin molecule soluble.
Valine is a hydrophobic amino acid. It does not interact with water molecules,
making the haemoglobin molecule less soluble.
When the abnormal haemoglobin is in an area of low oxygen concentration, the
haemoglobin molecules stick to one another, forming a big chain of molecules
that is not soluble and therefore forms long fibres. This pulls the red blood cells
(inside which haemoglobin is found) out of shape, making them sickle-shaped
instead of round. They are no longer able to move easily through the blood
system and may get stuck in capillaries. This is very painful and can be fatal.

Structure of transport
tissues
Plants can be very large, but they have a branching shape that helps to keep the
surface area-to-volume ratio fairly large. Their energy needs are generally small
compared with those of animals, so respiration does not take place so quickly.
They can therefore rely on diffusion to supply their cells with oxygen and to
remove carbon dioxide. Their leaves are very thin and have a large surface area
inside them in contact with the air spaces. This means that diffusion is sufficient
to supply the mesophyll cells with carbon dioxide for photosynthesis, and to
remove oxygen.
Plant transport systems, therefore, do not transport gases. Plants have two
transport systems:
l xylem, which transports water and inorganic ions from the roots to all other
parts of the plant
l phloem, which transports substances made in the plant, such as sucrose and
amino acids, to all parts of the plant

How to… Draw diagrams of plant transport tissues
An organ usually contains many different types of cell. These are Vascular bundle
arranged in a particular pattern characteristic of the organ, with Palisade layer
cells of a similar type found together, forming distinctive tissues. Xylem Phloem
Upper
Drawing plan diagrams epidermis
A plan diagram shows the outline of the various tissues in an
organ such as a leaf (Figure 7.1) or an eye. It does not show
individual cells.
You can use an eyepiece graticule to help you to show the
dimensions of the different tissues in the correct proportions.
For example, for the leaf, you could count the number of
eyepiece graticule divisions across the palisade layer, and the
number across the spongy layer, so that you can draw these Spongy layer Lower epidermis
the correct sizes in relation to one another. You do not need to
convert the eyepiece graticule divisions to real units (e.g. µm) so Figure 7.1 Plan diagram of a transverse section of a
there is no need to use a stage micrometer. dicotyledonous leaf
Drawing cellular detail
Rather than a plan diagram, you may be asked to draw details of
the cells you can see using a microscope. Use high power, and
draw just a few representative cells, showing details of organelles
if these are visible.
Xylem tissue Revised
Xylem tissue contains dead, empty cells with no end walls. These are called
xylem vessel elements. They are arranged in long lines to form xylem vessels
(Figure 7.2). These are long, hollow tubes through which water moves by mass
flow from the roots to all other parts of the plant.

Transverse section Longitudinal section
Cell wall containing cellulose and Pit in cell wall Dead cells have
lignin — lignin makes the wall allows movement no contents,
impermeable to water and provides of water out of allowing easy
strength, so the vessel element the vessel element movement of a
does not collapse when there is to other vessel continuous
negative pressure inside it elements or to column of water
neighbouring by mass flow
Narrow lumen increases area of water in contact tissues
with wall; water molecules adhere to the walls and
this helps to prevent breakage of the water column
Loss of end walls allows
continuous movement of a
column of water by mass flow
Figure 7.2 The structure of xylem vessels
Phloem tissue Revised
Phloem tissue contains cells called sieve tube elements. Unlike xylem vessel
elements, these are living cells and contain cytoplasm and a few organelles but
no nucleus. Their walls are made of cellulose. A companion cell is associated
with each sieve tube element (Figure 7.3).
Longitudinal section Transverse section

Sieve tube element with:
● Cell wall containing cellulose, with many
plasmodesmata forming direct links between the

cytoplasm of the sieve tube element and the
companion cell. Companion cell with:
● Cytoplasm containing some mitochondria and other ● Cytoplasm containing
organelles but no nucleus, leaving space for numerous organelles,

movement of phloem sap. including a nucleus and
● Companion cell many mitochondria
● Sieve plate — a perforated end wall allowing mass
flow of phloem sap through the sieve pores.
Figure 7.3 Phloem tissue
Transport mechanisms
Transport in xylem Revised
Figure 7.4 shows the pathway taken by water through a plant. The driving force
that causes this movement is the loss of water vapour from the leaves. This is Transpiration is the loss of water
called transpiration. vapour from a plant to its environment,
by diffusion down a water potential
Transpiration gradient, usually through the stomata
Transpiration is the loss of water vapour from a plant. Most transpiration in the leaf epidermis.
happens in the leaves. A leaf contains many cells in contact with air spaces in
the mesophyll layers. Liquid water in the cell walls changes to water vapour,
which diffuses into the air spaces. The water vapour then diffuses out of the leaf
through the stomata, down a water potential gradient, into the air surrounding Now test yourself
the leaf (Figure 7.5).
1 Use the diagram at the bottom left
Each stoma is surrounded by a pair of guard cells. These can change shape to in Figure 7.4 to draw a plan diagram
open or close the stoma. In order to photosynthesise, the stomata must be of a transverse section of a root.
open so that carbon dioxide can diffuse into the leaf. Plants cannot therefore Answer on p.203
avoid losing water vapour by transpiration. Tested
Transport in plants 49

5 In the leaves water moves 6 Water moves across the leaf
out of xylem vessels through by osmosis or by seeping
pits in the vessel walls. through cell walls. Water
evaporates from the surface
of leaf cells to form water
xylem
vapour inside air spaces.
phloem
7 Water vapour
diffuses through
4 Xylem vessels carry water the air spaces and
from the roots to the leaves. out of the leaf
The water moves through stomata.
by mass flow. xylem
phloem
path of one xylem vessel
3 At the endodermis,
water has to pass
through the cells
by osmosis. From root hair
there it enters
xylem vessels.
xylem
2 Water moves
from cell to cell phloem
by osmosis
through the cell
membranes, or cortex cells
between cells and
inside cell walls. root hair 1 Water enters root hairs by osmosis.
Figure 7.4 The pathway of water through a plant
Upper epidermis
Palisade mesophyll
Water evaporates
from cell walls
producing water
vapour. Xylem
Water vapour
diffuses down a Phloem
water potential
gradient. Spongy mesophyll
Water vapour Lower epidermis

diffuses out of
the leaf through
open stomata.
Guard cell Stoma Air space
Figure 7.5 Transpiration

Transpiration is affected by several factors:
l High temperature increases the rate of transpiration. This is because at
higher temperatures water molecules have more kinetic energy. Evaporation
from the cell walls inside the leaf therefore happens more rapidly, and
diffusion also happens more rapidly.
l High humidity decreases the rate of transpiration. This is because the water
potential gradient between the air spaces inside the leaf and the air outside is
less steep, so diffusion of water vapour out of the leaf happens more slowly.
l High wind speed increases the rate of transpiration. This is because the
moving air carries away water vapour from the surface of the leaf, helping
to maintain a water potential gradient between the air spaces inside the leaf
and the air outside.
l High light intensity may increase the rate of transpiration. This is because the
plant may be photosynthesising rapidly, requiring a rapid supply of carbon
dioxide. This means that more stomata are likely to be open, through which
water vapour can diffuse out of the leaf.

How to… Investigate the factors that affect transpiration rate
It is difficult to measure the rate at which water vapour is lost necessary, use a small piece of wire to fasten the tube tightly
from leaves. It is much easier to measure the rate at which a around the stem.
plant, or part of a plant, takes up water. Most of the water taken l Take the whole apparatus out of the water and support it
up is lost through transpiration, so we can generally assume that upright. Wait at least 10 minutes for it to dry out. If the glass
an increase in the rate of take-up of water indicates an increase tube is not marked with a scale, place a ruler or graph paper
in the rate of transpiration. behind it.
l Start a stop clock and read the position of the air/water
The apparatus used to measure the rate of take-up of water of
a plant shoot is called a potometer. This can simply be a long meniscus (which will be near the base of the tube). Record
glass tube. More complex potometers may have reservoirs, its position every 2 minutes (or whatever time interval seems
which make it easier to refill the tube with water, or a scale sensible). Stop when you have 10 readings, or when the
marked on them. meniscus is one third of the way up the tube.
l Fix a short length of rubber tubing over one end of the long l Change the environmental conditions and continue to
glass tube. Completely submerge the tube in water. Move it take readings. For example, you could use a fan to increase
around to get rid of all air inside it and fill it with water. Make ‘wind speed’, or move the apparatus into an area where the
absolutely sure there are no air bubbles. temperature is higher or lower.
l Take a leafy shoot from a plant and submerge it in the water l Plot distance moved by meniscus against time for each set
alongside the glass tube. Using a sharp blade, make a slanting of readings, on the same axes. Draw best fit lines. Calculate
cut across the stem. the mean distance moved per minute, or calculate the
l Push the cut end of the stem into the rubber tubing. Make slope of each line. This can be considered to be the rate of
sure the fit is tight and that there are no air bubbles. If transpiration.
How water moves from soil to air

Water moves from the soil to the air through a plant down a water potential
gradient (Figure 7.6). The water potential in the soil is generally higher than in
the air. The water potential in the leaves is kept lower than the water potential
in the soil because of the loss of water vapour by transpiration. Transpiration
maintains the water potential gradient.
l Water enters root hair cells by osmosis, moving down a water potential
gradient from the water in the spaces between soil particles, through the cell
surface membrane and into the cytoplasm and vacuole of the root hair cell.
l The water then moves from the root hair cell to a neighbouring cell by
osmosis, down a water potential gradient. This is called the symplastic
pathway.
l Water also seeps into the cell wall of the root hair cell. This does not involve
osmosis, as no partially permeable membrane is crossed. The water then
seeps into and along the cell walls of neighbouring cells. This is called the
apoplastic pathway. In most plant roots, the apoplastic pathway carries
more water than the symplastic pathway.

Low water
Symplastic pathway Apoplastic pathway
potential
Water vapour
in external air
Mass flow
Evaporation Water vapour Evaporation

in air spaces
Water in leaf
mesophyll cell walls
Water in leaf
mesophyll cell walls
Water in leaf
mesophyll cells
Water in
Osmosis xylem vessels Mass flow
Osmosis
Osmosis Water in Osmosis
endodermis cells
Water in Water in cortex

cortex cells cell walls
Osmosis Mass flow
Water in root Water in root

hair cells hair cell walls
High water Water in soil

Osmosis Mass flow
potential
Figure 7.6 Summary of water movement through a plant from soil to air
l When the water nears the centre of the root, it encounters a cylinder of cells
called the endodermis. Each cell has a ring of impermeable suberin around
it, forming the Casparian strip. This prevents water continuing to seep
through cell walls. It therefore travels through these cells by the symplastic
pathway.
l The water moves into the xylem vessels from the endodermis.
l Water moves up the xylem vessels by mass flow — that is, in a similar way to
water flowing in a river. The water molecules are held together by hydrogen
bonds between them, keeping the water column unbroken. This is called
cohesion, and it helps to create a tension in the water column as it is drawn
upwards. The water molecules are also attracted to the cellulose and lignin
in the walls of the xylem vessels, by a force called adhesion. There is a
relatively low hydrostatic pressure at the top of the column, produced by the
loss of water by transpiration. This lowering of hydrostatic pressure causes a
pressure gradient from the base to the top of the xylem vessel.
l In a leaf, water moves out of xylem vessels through pits, and then across the
leaf by the apoplastic and symplastic pathways.
l Water evaporates from the wet cell walls into the leaf spaces, and then
diffuses out through the stomata.
Xerophytes
A xerophyte is a plant that is adapted to live in an environment where water is
in short supply. The adaptations may include:
l leaves with a small surface area-to-volume ratio. This reduces the amount of
surface area from which water vapour can diffuse.
l leaves with a thick, waxy cuticle. This reduces the quantity of water that can
diffuse through the surface of the leaf into the air.

l methods of trapping moist air near the stomata, for example rolling the leaf
with the stomata inside, having stomata in pits in the leaf surface, having
hairs around the stomata. This produces a layer of high water potential
around the stomata, reducing the water potential gradient and therefore
reducing the rate of diffusion of water vapour from inside the leaf to outside.
Transport in phloem Revised
The movement of substances in phloem tissue is called translocation. The main

substances that are moved are sucrose and amino acids, which are in solution in
water. These substances have been made by the plant and are called assimilates.
How assimilates move through phloem tissue Revision activity

A part of a plant where assimilates such as sucrose enter the phloem is called a l Construct a table comparing the
source. A part where assimilates leave the phloem is called a sink. For example, structures and functions of xylem
a leaf may be a source and a root may be a sink. vessels and phloem sieve tubes.
Translocation of sucrose and other assimilates is an energy-requiring process.

l Respiration in companion cells at a source provides ATP that is used to fuel
the transport of sucrose into the companion cell. This is done by pumping
protons (hydrogen ions) out of the companion cell. As the protons diffuse
back into the companion cell, they pass through a co-transporter protein
that allows both protons and sucrose molecules to enter the cell. The
protons carry sucrose molecules with them. This increases the concentration
of sucrose in the companion cell, so that it moves by diffusion down a
concentration gradient, through the plasmodesmata, into the phloem sieve
element.
l The increased concentration of sucrose in the companion cell and phloem
sieve element produces a water potential gradient from the surrounding cells
into the companion cell and phloem sieve element. Water moves down this
gradient.
l At a sink, sucrose diffuses out of the phloem sieve element and down a
concentration gradient into a cell that is using sucrose. This produces a water
potential gradient, so water also diffuses out of the phloem sieve element.
l The addition of water at the source and the loss of water at the sink
produces a higher hydrostatic pressure inside the phloem sieve element at
the source than at the sink. Phloem sap therefore moves by mass flow
down this pressure gradient, through the phloem sieve elements and
through the sieve pores, from source to sink.
2 At which of these stages of transport of sucrose does the plant have to provide energy?
a loading sucrose into the phloem sieve tube
b mass flow of phloem sap from source to sink
3 Explain why the contents of xylem vessels always flow upwards from roots to leaves, while the contents of phloem sieve
tubes can flow either upwards or downwards.
4 Plants contain many different organs, including flowers, leaves, roots and fruits. In a temperate country, different organs
in a plant are sources and sinks at different times of year. For each of the following, state which of these organs will be
sources and which will be sinks:
a in summer, when there is plenty of sunlight and flower buds are beginning to open
b in autumn, when there is still plenty of sunlight and the plant is building up stores of starch in its roots ready for the
winter; flowers have been fertilised and fruits are developing
c in winter, when there is little sunlight, and the plant relies on starch stores in its roots
d in spring, when the leaves are just starting to grow
Answers on p.203

The circulatory system

Mammals have a blood system made up of blood vessels and the heart. The
heart produces high pressure, which causes blood to move through the vessels
by mass flow. The blood system is called a closed system because the blood Expert tip
travels inside vessels. It is called a double circulatory system because the Mass flow means that a whole body
blood flows from the heart to the lungs, then back to the heart, then around of liquid flows together, like a river. It
the rest of the body and then back to the heart again. The vessels taking blood is much faster than diffusion, where
individual molecules or ions move
to the lungs and back make up the pulmonary system. The vessels taking
randomly.
blood to the rest of the body and back make up the systemic system.
Blood vessels Revised
Arteries carry blood away from the heart. The blood that flows through them
is pulsing and at a high pressure. They therefore have thick, elastic walls, which
can expand and recoil as the blood pulses through. The artery wall also contains
Typical mistake
variable amounts of smooth muscle. Arteries branch into smaller vessels called
arterioles. These also contain smooth muscle in their walls, which can contract Do not state that the muscle in artery
walls pumps blood through them
and make the lumen (space inside) smaller. This helps to control the flow of
– this is not correct.
blood to different parts of the body.
Capillaries are tiny vessels with just enough space for red blood cells to squeeze
through. Their walls are only one cell thick, and there are often gaps in the walls
through which plasma (the liquid component of blood) can leak out. Capillaries
deliver nutrients, hormones and other requirements to body cells, and take away
their waste products. Their small size and thin walls minimise diffusion distance,
enabling exchange to take place rapidly between the blood and the body cells.
Veins carry low-pressure blood back to the heart. Their walls do not need to be
as tough or as elastic as those of arteries as the blood is not at high pressure and is
not pulsing. The lumen is larger than in arteries (Figure 8.1), reducing friction, which
would otherwise slow down blood movement. They contain valves, to ensure
that the blood does not flow back the wrong way. Blood is kept moving through
many veins, for example those in the legs, by the squeezing effect produced by
contraction of the body muscles close to them, which are used when walking.
Figure 8.2 shows the main blood vessels in the human body.
Artery Vein Capillary

Connective tissue Connective tissue
Fibrous tissue Fibrous tissue
Smooth muscle Smooth muscle
Elastic fibres and elastic fibres
Endothelial
Lumen Endothelium Lumen Endothelium Lumen cell
Figure 8.1 Structure of blood vessels

Capillary network in Carotid artery
head, neck and arms
Superior vena cava Pulmonary vein
Capillary network Aorta

in lungs
Pulmonary artery Left atrium
Right atrium Left ventricle
Right ventricle Hepatic artery (to liver)
Mesenteric artery
Inferior vena cava
Now test yourself
Hepatic vein Capillary network in gut 1 Do all arteries carry oxygenated
(from liver) blood? Explain your answer.
Hepatic portal vein

Capillary network Answer on p.203
in body and legs
Tested
Figure 8.2 The main blood vessels in the human body
Pressure changes in the circulatory system Revised
The pressure of the blood changes as it moves through the circulatory system
(Figure 8.3).
l In the arteries, blood is at high pressure because it has just been pumped
out of the heart. The pressure oscillates (goes up and down) in time with
the heart beat. The stretching and recoil of the artery walls helps to smooth
the oscillations, so the pressure becomes gradually steadier the further the
blood moves along the arteries. The mean pressure also gradually decreases,
particularly as the blood flows through arterioles (small arteries).
l The total cross-sectional area of the capillaries is greater than that of the
arteries that supply them, so blood pressure is less inside the capillaries than
inside arteries.
l In the veins, blood is at a very low pressure, as it is now a long way from the
pumping effect of the heart.
Blood 120
Arterioles Pulmonary
pressure/
100 arteries
mmHg Capillaries
80 Arterioles
Venules
Aorta Capillaries
60 Smaller
Smaller veins Venules
40 arteries Vena Pulmonary
20 cava veins
0
Left side of heart Right side of heart
Figure 8.3 Pressure changes in the circulatory system
Transport in mammals 55

Blood
Revised
Blood contains the following cell types (Figure 8.4).

l Red blood cells. These transport oxygen from lungs to respiring tissues.
They are very small and have the shape of a biconcave disc. This increases
their surface area-to-volume ratio, allowing rapid diffusion of oxygen into
and out of them. They contain haemoglobin, which combines with oxygen
to form oxy-haemoglobin in areas of high concentration (such as the lungs)
and releases oxygen in areas of low concentration (such as respiring tissues).
They do not contain a nucleus or mitochondria.
l Phagocytes. These are white blood cells that engulf and digest unwanted
cells, such as damaged body cells or pathogens. They are larger than red
blood cells. They include monocytes and neutrophils.
l Lymphocytes. These are white blood cells that respond to particular
pathogens by secreting antibodies or by directly destroying them. Each
lymphocyte is able to recognise one particular pathogen and respond to it
by secreting one particular type of antibody or by attacking it. You can find
out more about this on pp. 69–71.
Red blood cells — flattened White blood cells contain a nucleus which is usually stained dark blue when a blood
discs,thinnest at the middle. smear is prepared for viewing under a microscope. Several forms of white cells can be
recognised, such as lymphocytes, monocytes and neutrophils.
Lymphocyte Monocyte — the largest white

— nucleus filling cell, often with a kidney-shaped
Top view Side view most of the cell. nucleus.
Phagocytes
Platelets — small Neutrophil — has a lobed nucleus
structures usually (often 3–5 lobes) and granules in the
stained blue. cytoplasm that may be stained pink.
Figure 8.4 Blood cells
Haemoglobin and oxygen transport Revised
Haemoglobin (Hb) is a protein with quaternary structure. A haemoglobin

molecule is made up of four polypeptide chains, each of which has a haem
group at its centre. Each haem group contains an Fe2+ ion, which is able to
combine reversibly with oxygen, forming oxyhaemoglobin. Each iron ion can
combine with two oxygen atoms, so one Hb molecule can combine with eight
oxygen atoms.
Oxygen concentration can be measured as partial pressure, in kilopascals (kPa).
Haemoglobin combines with more oxygen at high partial pressures than it does
at low partial pressures. At high partial pressures of oxygen, all the haemoglobin
will be combined with oxygen, and we say that it is 100% saturated with oxygen.
A graph showing the relationship between the partial pressure of oxygen
and the percentage saturation of haemoglobin with oxygen is known as a
dissociation curve (Figure 8.5). In the lungs, the partial pressure of oxygen may
be around 12 kPa. You can see from the graph that the Hb will be about 98%
saturated. Expert tip
When explaining how the structure of
In a respiring muscle, the partial pressure of oxygen may be around 2 kPa. The haemoglobin is related to its functions,
Hb will be about 23% saturated. remember that its ability to unload
Therefore, when Hb from the lungs arrives at a respiring muscle it gives up more oxygen is just as important as its ability
to combine with it.
than 70% of the oxygen it is carrying.

Percentage 100
saturation
of Hb with
oxygen 80
The steepest part of the curve indicates where
60 relatively small changes in partial pressure of
oxygen cause the greatest loading or unloading
of Hb with oxygen.
40
20
0
0 2 4 6 8 10 12 14
Partial pressure of oxygen/kPa
Figure 8.5 The oxygen dissociation curve for haemoglobin
The Bohr effect

The presence of carbon dioxide increases acidity, that is, the concentration of
H+ ions. When this happens, the haemoglobin combines with H+ ions and
releases oxygen.
Red blood cells contain an enzyme called carbonic anhydrase, which catalyses
the reaction of carbon dioxide and water to form carbonic acid:
carbonic
anhydrase
CO2 + H2O H2CO3
The carbonic acid then dissociates:
H2CO3 ⇌ H+ + HCO3−
The hydrogen ions combine with haemoglobin to form haemoglobinic acid.
This causes the haemoglobin to release oxygen.
Therefore, in areas of high carbon dioxide concentration, haemoglobin is less
saturated with oxygen than it would be if there was no carbon dioxide present.
This is called the Bohr effect (Figure 8.6). It is useful in enabling haemoglobin to
unload more of its oxygen in tissues where respiration (which produces carbon
dioxide) is taking place.
Percentage 100
saturation
of Hb with
oxygen 80
Key
60 lower concentration
of carbon dioxide
40 higher concentration
of carbon dioxide
20
0
0 2 4 6 8 10 12 14
Partial pressure of oxygen/kPa
Figure 8.6 The Bohr effect
Transport of carbon dioxide

Carbon dioxide is transported in the blood in three different ways:
l 85% as hydrogencarbonate ions, HCO3−, as described above
l 10% combined with haemoglobin, to form carbaminohaemoglobin
l 5% in solution in blood plasma

Adaptation to high altitude
At high altitudes, the air is less dense and the partial pressure of oxygen is lower
than at sea level. Haemoglobin is therefore less saturated with oxygen in the
lungs and delivers less oxygen to body tissues.
After some time at high altitude, the number of red blood cells in the blood
increases. This means that there are more haemoglobin molecules in a given
volume of blood. Therefore, even though each Hb molecule carries less oxygen
on average than at sea level, the fact that there are more of them helps to
supply the same amount of oxygen to respiring tissues.
Athletes can make use of this by training at high altitude before an important
competition. When they return to low altitude, their extra red blood cells can
supply oxygen to their muscles at a greater rate than in an athlete who has not
been to high altitude, giving them a competitive advantage.
Tissue fluid and lymph Revised
Capillaries have tiny gaps between the cells in their walls. Near the arteriole end
of a capillary, there is relatively high pressure inside the capillary, and plasma leaks
out through these gaps to fill the spaces between the body cells. This leaked
plasma is called tissue fluid.
Tissue fluid is therefore very similar to blood plasma. However, very large
molecules such as albumin (a protein carried in solution in blood plasma) and
other plasma proteins cannot get through the pores and so remain in the blood
plasma.
The tissue fluid bathes the body cells. Substances such as oxygen, glucose or
urea can move between the blood plasma and the cells by diffusing through the
tissue fluid.
Some tissue fluid moves back into the capillaries, becoming part of the blood
plasma once more. This happens especially at the venule end of the capillary
where blood pressure is lower, producing a pressure gradient down which the
tissue fluid can flow. However, some of the tissue fluid collects into blind-ending
vessels called lymphatic vessels. It is then called lymph.
Lymphatic vessels have valves that allow fluid to flow into them and along them
but not back out again. They carry the lymph towards the subclavian veins (near
the collarbone) where it is returned to the blood. The lymph passes through Revision activity
lymphatic glands where white blood cells accumulate. Lymph therefore tends l Construct a table to compare
to carry higher densities of white blood cells than are found in blood plasma or blood, tissue fluid and lymph.
tissue fluid.
The heart
The heart of a mammal (Figure 8.7) has four chambers. The two atria receive
blood, and the two ventricles push blood out of the heart (Figure 8.8). The
atria and ventricle on the left side of the heart contain oxygenated blood, while
those on the right side contain deoxygenated blood. The walls of the heart are
made of cardiac muscle.

Aorta
Superior vena cava
Pulmonary artery
Pulmonary veins Left atrium
Right atrium
Coronary arteries
Right ventricle
Left ventricle
Inferior vena cava
Figure 8.7 External view of a mammalian heart
Aorta Pulmonary
artery
Superior
vena cava Pulmonary vein
Right atrium Left atrium

Atrioventricular
Atrioventricular
valve Key
valve
Left ventricle Oxygenated
Right ventricle blood
Inferior Septum Deoxygenated
vena cava blood
Note: the walls of the ventricles are thicker than those of the atria because
the cardiac muscle has to produce more pressure. The wall of the left ventricle
is thicker than the wall of the right ventricle because the right ventricle only
has to push blood through the capillaries of the lungs, where the resistance
to flow is much less than through all the capillaries of the body organs
supplied with blood from the left ventricle.
Figure 8.8 Vertical section through a mammalian heart
The cardiac cycle Revised
When muscle contracts, it gets shorter. Contraction of the cardiac muscle in the
walls of the heart therefore causes the walls to squeeze inwards on the blood
inside the heart. Both sides of the heart contract and relax together. The complete
sequence of one heart beat is called the cardiac cycle (Figures 8.9 and 8.10).
During atrial systole, the muscle in the

walls of the atria contracts, pushing more
Atria
blood into the ventricles through the
contract
open atrioventricular valves.
Semilunar During ventricular systole, the muscle in

valves open the walls of the ventricles contracts. This
Atrioventricular causes the pressure of the blood inside the
valves close ventricles to become greater than in the
atria, forcing the atrioventricular valves
Ventricles shut. The blood is forced out through the
contract aorta and pulmonary artery.
Semilunar During diastole, the heart muscles relax.

valves close The pressure inside the ventricles becomes Expert tip
less than that inside the aorta and Remember that the valves are
pulmonary artery, so the blood inside opened and closed by differences in
these vessels pushes the semilunar valves blood pressure. They cannot move
Atrioventricular shut. Blood flows into the atria from the themselves.
valves open veins, so the cycle is ready to begin again.
Figure 8.9 The cardiac cycle

Atrial Ventricular Diastole Atrial
systole systole systole
Key
Pressure/ 16
Aorta
kPa 14
Left
12 ventricle
10 Right
8 ventricle
6 Left Now test yourself
4 atrium
2 2 Use Figure 8.10 to calculate the
number of heart beats in 1 minute.
0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 Answer on p.203
Time/s Tested
Figure 8.10 Pressure changes during the cardiac cycle
Initiation and control of the cardiac cycle

Cardiac muscle is myogenic — that is, it contracts and relaxes automatically,
without the need for stimulation by nerves. The rhythmic contraction of
the cardiac muscle in different parts of the heart is coordinated by electrical
impulses passing through the cardiac muscle tissue.
l In the wall of the right atrium, there is a patch of muscle tissue called the
sinoatrial node (SAN). This has an intrinsic rate of contraction a little
higher than that of the rest of the heart muscle.
l As the cells in the SAN contract, they generate action potentials (electrical
impulses — see p. 130) which sweep along the muscle in the wall of the
right and left atria. This causes the muscle to contract. This is atrial systole.
l When the action potentials reach the atrioventricular node (AVN) in
the septum, they are delayed briefly. They then sweep down the septum
between the ventricles, along fibres of Purkyne tissue, and then up through
the ventricle walls. This causes the ventricles to contract slightly after the
atria. The left and right ventricles contract together, from the bottom
up. This is ventricular systole.
l There is then a short delay before the next wave of action potentials is
generated in the SAN. During this time, the heart muscles relax. This is
diastole.

All organisms take in gases from their environment and release gases to the
environment. Animals take in oxygen for aerobic respiration and release carbon
dioxide. Plants also respire, but during daylight hours they photosynthesise at a
greater rate than they respire, and so take in carbon dioxide and release oxygen.
Gas exchange is the movement of
The body surface across which these gases diffuse into and out of the body is gases into and out of an organism’s
called the gas exchange surface. In mammals, including humans, the gas body, across a gas exchange surface.
exchange surface is the surface of the alveoli in the lungs.
The human gas exchange

system
Structure of the human gas exchange system Revised
Figure 9.1 shows the structure of the human gas exchange system.
Human gas Trachea Ciliated epithelium lining trachea,

exchange system bronchi and some bronchioles
Hodder CIE Revision 10 Cartilage
modified from Cilated epithelial cell Cilia
Edxl Jul04-Aug05 Bronchus
Rib
External intercostal muscle Mucus
Internal intercostal muscle

Bronchiole Goblet
Basement
membrane cell
Alveolus
Diaphragm
Cross sections of airways to show the distribution Alveolus

of tissues (not to scale)
Trachea Key
Connective tissue
Cartilage
Smooth muscle
Ciliated epithelium
Bronchus Bronchiole Thin layer of

fluid containing
surfactant
Alveolar wall
one cell thick
Elastic fibres Capillary wall (squamous
one cell thick epithelium)
Figure 9.1 The structure of the human gas exchange system
Gas exchange and smoking 61

Cartilage in the walls of the trachea and bronchi provides support and prevents
the tubes collapsing when the air pressure inside them is low.
Ciliated epithelium is found lining the trachea, bronchi and some bronchioles.
It is a single layer of cells whose outer surfaces are covered with many thin
extensions (cilia), which are able to move. They sweep mucus upwards towards
the mouth, helping to prevent dust particles and bacteria reaching the lungs.
Goblet cells are also found in the ciliated epithelium. They secrete mucus,
which traps dust particles and bacteria.
Smooth muscle cells are found in the walls of the trachea, bronchi and
bronchioles. This type of muscle can contract slowly but for long periods
without tiring. When it contracts, it reduces the diameter of the tubes. During
exercise it relaxes, widening the tubes so more air can reach the lungs.
Elastic fibres are found in the walls of all tubes and between the alveoli. When
breathing in, these fibres stretch to allow the alveoli and airways to expand.
When breathing out, they recoil, helping to reduce the volume of alveoli and
expel air out of the lungs.
Gas exchange at the alveolar surface Revised
The air inside an alveolus contains a higher concentration of oxygen, and a

lower concentration of carbon dioxide, than the blood in the capillaries. This
blood has been brought to the lungs in the pulmonary artery, which carries
deoxygenated blood from the heart. Oxygen therefore diffuses from the
alveolus into the blood capillary, through the thin walls of the alveolus and the
capillary. Carbon dioxide diffuses from the capillary into the alveolus.
The diffusion gradients for these gases are maintained by:
l breathing movements, which draw air from outside the body into the lungs,
and then push it out again; this maintains a relatively high concentration of
oxygen and low concentration of carbon dioxide in the alveoli
l blood flow past the alveolus, which brings deoxygenated blood and carries
away oxygenated blood
Cigarette smoking and

health
The smoke from cigarettes contains several substances that affect the gas
exchange system and the cardiovascular system. These include:
l tar, a mixture of substances including various chemicals that act as
carcinogens.
l nicotine, an addictive substance that affects the nervous system by binding
to receptors on neurones (nerve cells) in the brain and other parts of the
body. It increases the release of a neurotransmitter called dopamine in the
brain, which gives feelings of pleasure. It increases the release of adrenaline
into the blood, which in turn increases breathing rate and heart rate. There
is also some evidence that nicotine increases the likelihood of blood clots
forming.
Typical mistake
l carbon monoxide, which combines irreversibly with haemoglobin, forming
carboxyhaemoglobin. This reduces the amount of haemoglobin available to Do not confuse carboxyhaemoglobin
with carbaminohaemoglobin
combine with oxygen, and so reduces the amount of oxygen that is
(see p. 5).
transported to body tissues.

Effects of smoking on the gas exchange system

Revised
Chronic obstructive pulmonary disease (COPD)

This is a condition in which a person has chronic bronchitis and emphysema.
It can be extremely disabling, as it is difficult to get sufficient oxygen into the
blood to support activity.
Chronic bronchitis
Various components of cigarette smoke, including tar, cause goblet cells to
increase mucus production and cilia to beat less strongly. This causes mucus
to build up, which may partially block alveoli. This makes gas exchange more
difficult, as the diffusion distance between the air in the alveoli and the blood in
the capillaries is greater. The mucus may become infected with bacteria, causing
bronchitis. Smokers often have chronic (long-lasting) bronchitis.
The mucus stimulates persistent coughing, which can damage the tissues in the
walls of the airways, making them stiffer and the airways narrower.
Emphysema
Smoking causes inflammation in the lungs. This involves the presence of
increased numbers of white blood cells, some of which secrete chemicals that
damage elastic fibres. This makes the alveoli less elastic. They may burst, resulting
in larger air spaces. This reduces the surface area available for gas exchange.
This is called emphysema. A person with emphysema has shortness of breath,
meaning they struggle to breathe as deeply as they need to, especially when
exercising.
Lung cancer
Various components of tar can cause changes in the DNA in body cells,
including the genes that control cell division, which can cause cancer. These
substances are therefore carcinogens. Cancers caused by cigarette smoke are
most likely to form in the lungs but may form anywhere in the gas exchange
system, and also in other parts of the body. Smoking increases the risk of
developing all types of cancer. Symptoms of lung cancer include shortness of
breath, a chronic cough — which may bring up blood — chest pain, fatigue and
weight loss.
Effects of smoking on the cardiovascular system Revised
The nicotine and carbon monoxide in tobacco smoke increase the risk of
developing atherosclerosis. Atherosclerosis is a thickening and loss of elasticity
in the walls of arteries. It is caused by build-up of plaques in the blood vessel
wall. The plaques contain cholesterol and fibres. They produce a rough surface
lining the artery, which stimulates the formation of blood clots.
A blood clot may break away from the artery wall and get stuck in a narrow
vessel elsewhere in the blood system, for example in the lungs or in the brain.
This prevents blood passing through so cells are not supplied with oxygen and
die. If this happens in the brain it is called a stroke.
The loss of elasticity in an artery or arteriole also makes it more likely that the
vessel will burst when high-pressure blood pulses through. This is another cause
of stroke.
Revision activity
If atherosclerosis happens in the coronary arteries that supply the heart muscle
with oxygenated blood, the person has coronary heart disease (CHD). Parts of l Construct a spider diagram to
show the different components of
the muscle may be unable to function properly as they do not have enough cigarette smoke, and the effects
oxygen for aerobic respiration. The muscle may die. Eventually, this part of the that they have on health.
heart might stop beating, causing a heart attack.
Gas exchange and smoking 63

Disease can be defined as a condition in which the body does not function
normally, and which produces unpleasant symptoms such as pain, distress or
A disease is a disorder of the body
feeling weak. The term disease is generally used for conditions that last for at that leads to ill health.
least several days.
An infectious disease is one that can be passed between one person and
another. Infectious diseases are caused by pathogens. These are usually
microorganisms such as viruses, bacteria, fungi or protoctists.
A non-infectious disease cannot be passed between people and is not caused
by pathogens. Examples include sickle cell anaemia and lung cancer.
Important infectious
diseases
Cholera Revised
Cause
The cause of an infectious disease
Cholera is caused by a bacterium, Vibrio cholerae.
is the pathogen that produces the
Transmission symptoms of the disease.
V. cholerae can enter the body in contaminated food or water. The bacteria
breed in the small intestine, where they secrete a toxin that reduces the ability of
the epithelium of the intestine to absorb salts and water into the blood. These Transmission is the movement of a
are lost in the faeces, causing diarrhoea. If not treated, the loss of fluid can be pathogen from an infected person to
fatal. Cholera is most likely to occur where people use water or food that has an uninfected person.
been in contact with untreated sewage, as the bacteria are present in the faeces
of an infected person.
Prevention and control

Transmission is most likely to occur in crowded and impoverished conditions,
such as refugee camps. Cholera is best controlled by treating sewage effectively,
providing a clean water supply and maintaining good hygiene in food
preparation. There is no fully effective vaccine for cholera.
Malaria Revised
Cause
Malaria is caused by a protoctist, Plasmodium. There are several species, which
cause different types of malaria. In a person, the Plasmodium infects red blood
cells and breeds inside them. Toxins are released when the Plasmodium burst
out of the cells, causing fever.

Transmission
A vector is an organism that transmits
Plasmodium is transmitted in the saliva of female Anopheles mosquitoes, which a pathogen from one host to another.
inject saliva to prevent blood clotting when they feed on blood from a person.
When a mosquito bites an infected person, Plasmodium is taken up into the
mosquito’s body and eventually reaches its salivary glands. The mosquito is said Typical mistake
to be a vector for malaria. Mosquitoes are not the cause
of malaria. Malaria is caused by
Prevention and control Plasmodium. Mosquitoes are the
Reducing the population of mosquitoes, for example by removing sources of vector for this disease.
water in which they can breed, or by releasing large numbers of sterile males, can
reduce the transmission of malaria.
Now test yourself
Preventing mosquitoes from biting people, for example by sleeping under a
mosquito net, or by wearing long-sleeved clothing and insect repellant, can 1 Explain the difference between the
cause of an infectious disease, and
reduce the chances of a mosquito picking up Plasmodium from an infected
a vector for an infectious disease.
person, or passing it to an uninfected person.
Prophylactic drugs (that is, drugs that prevent pathogens infecting and breeding
Answer on p.203
Tested
in a person) can be taken. However, in many parts of the world Plasmodium has
evolved resistance to some of these drugs.
Tuberculosis (TB) Revised
Cause
TB is caused by the bacterium Mycobacterium tuberculosis or (more rarely)
Mycobacterium bovis.
Transmission
M. tuberculosis can enter the lungs in airborne droplets of liquid that are
breathed in. This is more likely to happen in places where many people are living
in crowded conditions.

TB is most prevalent amongst people living in poor accommodation, or whose
immune systems are not functioning well, perhaps because of malnutrition or
infection with HIV (see below). Increasing standards of living and treating HIV
infection can therefore help to reduce the incidence of TB.
Vaccination with the BCG vaccine confers immunity to TB in many people. New
vaccines are being developed that it is hoped will be more effective.
Treatment of HIV with drug therapy reduces the risk that an HIV-positive person
will get TB.
Treatment of TB with antibiotics can often completely cure the disease.
However, this is not always the case because:
l there are now many strains of the M. tuberculosis bacterium that have
evolved resistance to most of the antibiotics that are used
l the bacteria reproduce inside body cells, where it is difficult for drugs to
reach them
l the drugs need to be taken over a long time period, which often requires a
health worker checking that a person takes their drugs every day
Infectious disease 65

HIV/AIDS
Revised
Cause
AIDS (acquired immunodeficiency syndrome) is caused by the human
immunodeficiency virus, HIV. This is a retrovirus, which contains RNA. The
virus enters T-lymphocytes (p. 71), where its RNA is used to make viral DNA,
which is incorporated into the T-lymphocytes’ chromosomes. A person who
has been infected with HIV makes antibodies against the virus, and is said to be
HIV-positive. Usually nothing more happens for several years after infection, but
eventually multiple copies of the virus are made inside the T-lymphocytes, which
are destroyed as the viruses break out and infect more cells. Eventually there
are so few functioning T-lymphocytes that the person is no longer able to resist
infection by other pathogens and develops one or more opportunistic diseases
such as TB. The person has AIDS.
Transmission
HIV can be passed from one person to another through:
l blood from one person entering that of another, for example by sharing
hypodermic needles, or through blood transfusions
l exchange of fluids from the penis, vagina or anus
l across the placenta from mother to fetus, or in breast milk

All blood to be used in transfusions should be screened to ensure it does not
contain HIV.
All hypodermic needles should be sterile and used only once, and disposed of
carefully.
A person should avoid sexual activity with anyone whose HIV status they do
not know. If everyone had only one partner, HIV could not be transmitted.
Condoms, if properly used, can prevent the virus passing from one person to
another during intercourse. If a person is diagnosed with HIV, all their sexual
contacts should be traced and informed that they may have the virus.
The chance of HIV passing from an HIV-positive mother to her fetus is greatly
reduced if the mother is treated with appropriate drugs. These drugs can also Now test yourself
greatly increase the length of time between a person becoming infected with
2 Explain the difference between
HIV and developing symptoms of AIDS, and can significantly prolong life. being HIV-positive and having
HIV infection rates are especially high in sub-Saharan Africa. Many of these AIDS.
people are not able to receive treatment with effective drugs, generally for Answer on p.203
economic reasons. Tested
Smallpox Revised
Cause
Smallpox is caused by the variola virus.
Transmission
Transmission occurs by the inhalation of droplets of moisture containing the
virus.

Smallpox was a serious disease that was fatal in 20–60% of adults who caught it,
and in an even higher percentage of infected children. It was eradicated by 1979,
through a vaccination campaign coordinated by the World Health Organization.

Measles
Revised
Cause
Measles is caused by a morbillivirus.
Transmission
Transmission occurs through the inhalation of droplets of moisture containing
the virus. It is highly infectious, so a high proportion of people who come into
close contact with an infected person will also get the disease.

Measles is a serious disease, which can cause death, especially in adults and
in people who are not in good health, for example because they do not have
access to a good diet. Vaccination is the best defence against measles. The
vaccine is highly effective, especially if two doses are given. The people most
likely to suffer from measles are therefore those who are malnourished and who
live in areas where no vaccination programme is in place.
Global patterns of disease Revised
Malaria is found in parts of the world where the Anopheles mosquito species
that can act as vectors are found. This is mostly in tropical and subtropical
regions where humidity is high (Figure 10.1).
Key
Distribution
of malaria
Figure 10.1 Global distribution of malaria
TB is found in all countries of the world, including developed countries such as
the USA and the UK (Figure 10.2). However, it is most common in areas where
living conditions are poor and crowded, or where large numbers of people have
HIV/AIDS (Figure 10.3).
Key
Cases per 100 000
<10 10–50 51–100 101–300 >300 per year
Figure 10.2 Global distribution of TB
Infectious disease 67

Revision activity
l Construct a table that shows the

cause, method of transmission, and
prevention and control methods
for each of the infectious diseases
described on pp. 64–67.
Key
Cases per 1000
<10 1–4 5–9 10–49 50–149 150–390 people per year
Figure 10.3 Global distribution of HIV/AIDS
Antibiotics
How antibiotics work Revised
An antibiotic is a substance that, when taken orally or by injection, kills bacteria

but does not harm human cells. Antibiotics are not effective against viruses.
Many antibiotics are originally derived from fungi, but they can also be obtained
from other organisms (for example, amphibian skin or plants) or synthesised in
the laboratory.
Antibiotics act on structures or metabolic pathways that are found in bacteria
but not in eukaryotic cells. The correct antibiotic must be chosen for a particular
disease. For example:
l penicillin prevents the synthesis of the links between peptidoglycan
molecules in bacterial cell walls; when the bacteria take up water by osmosis,
the cell wall is not strong enough to prevent them bursting
l rifampicin (rifampin) inhibits an enzyme required for RNA synthesis in
bacteria
l tetracycline binds to bacterial ribosomes and inhibits protein synthesis
Resistance to antibiotics Revised
Exposure to antibiotics exerts strong selection pressure on bacterial populations.

Any bacterium that is resistant to the antibiotic — for example, because it has
an allele of a gene that causes the synthesis of an enzyme that can break down
the antibiotic — has a selective advantage and is more likely to survive and
reproduce successfully. The offspring will inherit the alleles that confer resistance.
A whole population of resistant bacteria can therefore be produced.
For this reason, it is important that antibiotics are only used when necessary. A
person prescribed antibiotics should complete the course, as this increases the
chances of eradicating all the disease-causing bacteria in the body. Using more
than one antibiotic also reduces the chance of resistance developing, because it
is unlikely that any one bacterium will possess two different resistance alleles.

11 Immunity
The immune system

The human immune system is made up of the organs and tissues involved in
destroying pathogens inside the body. There are two main groups of cells involved:
l phagocytes, which ingest and digest pathogens or infected cells
l lymphocytes, which recognise specific pathogens through interaction with
receptors in their cell surface membranes, and respond in one of several
ways, for example by secreting antibodies
The numbers of both of these types of white blood cells increase when a person
has an infectious disease, because mitosis of lymphocytes (see below) occurs as
a result of contact with a pathogen. A type of cancer, called leukaemia, results
from uncontrolled division of the stem cells that produce white blood cells, and
this also causes an increase in their numbers. This can mean that the blood no
longer has enough red blood cells, so oxygen transport is impaired.
Phagocytes Revised
Phagocytes are produced in the bone marrow by the mitotic division

of precursor cells. This produces cells that develop into monocytes or
neutrophils (Figure 11.1).
Monocytes are inactive cells that circulate in the blood. They eventually leave
the blood, often as the result of encountering chemical signals indicating that
bacteria or viruses are present. As monocytes mature, they develop more
RER, Golgi bodies and lysosomes. When they leave the blood they become
macrophages. They engulf bacteria by endocytosis (p. 38) and digest them
inside phagosomes. Monocytes and macrophages can live for several months.
Similar precursor cells in bone marrow produce neutrophils. These also travel in
blood. They leave the blood in large numbers at sites of infection and engulf and
digest bacteria in a similar way to macrophages. A neutrophil lives for only a few
days.
Monocyte Neutrophil
Figure 11.1 Mature phagocytes

Phagocytes are able to act against any invading organisms. Their response is non-
specific.
The immune response Revised
Lymphocytes, unlike phagocytes, act against specific pathogens. Each

lymphocyte contains a set of genes that codes for the production of a particular
type of receptor. We have many million different types, each producing just one
type of receptor.
Both B-lymphocytes and T-lymphocytes are made in bone marrow.
B-lymphocytes then spread through the body and settle in lymph nodes,
Immunity 69

although some continue to circulate in the blood. T-lymphocytes collect in
11 Immunity
the thymus gland, where they mature before spreading into the same areas as
B-lymphocytes. The thymus gland disappears at around the time of puberty.
Both types of lymphocyte have a large, rounded nucleus that takes up most of
the cell. They can only be told apart by their different actions (see below).
An antigen is any molecule, usually a
During the maturation process, any lymphocytes that produce receptors that
protein or glycoprotein, that is different
would bind with those on the body’s own cells are destroyed. This means that from the molecules in the cell-surface
the remaining lymphocytes will only act against non-self molecules that are not membranes of our own body cells and
normally found in the body. Non-self molecules, such as those on the surfaces therefore elicits an immune response.
of invading bacteria, are called antigens.
Several different types of cell, including macrophages, place antigens of
pathogens they have encountered in their cell surface membranes, where there
is a good chance that a B-lymphocyte or T-lymphocyte may encounter them.
These cells are called antigen-presenting cells.
Action of B-lymphocytes
A B-lymphocyte places some of its specific receptor molecules in its cell surface
membrane. If it encounters an antigen that binds with this receptor, the
B-lymphocyte is activated. It divides repeatedly by mitosis to produce a clone of An antibody is a protein molecule
genetically identical plasma cells. Some of these synthesise and secrete large secreted by a plasma cell that binds
quantities of proteins called immunoglobulins or antibodies. The antibodies with specific antigens.
have the same binding sites as the specific receptors in the B-lymphocyte’s
membrane, so they can bind with the antigens. This may directly destroy or
neutralise the antigens, or it may make it easier for phagocytes to destroy them. Now test yourself
Some of the clone of B-lymphocyte cells become memory cells. These remain 1 B-lymphocytes have large numbers
in the blood for many years. They are able to divide rapidly to produce plasma of mitochondria, extensive rough
endoplasmic reticulum and several
cells if the same antigen invades the body again. More antibody is therefore Golgi bodies. Explain why this is so.
secreted more rapidly than when the first invasion happened, and it is likely that
the pathogens will be destroyed before they have a chance to reproduce. The Answer on p.203
person has become immune to this pathogen (Figure 11.2). Tested
Bacterium
B cell displays a receptor specific
Antigen-presenting cell to the antigen on the bacterium
Antibody
Phagocytosis by an
antigen-presenting
cell, e.g. a macrophage The B cell meets its specific antigen,
either on a macrophage or on the
bacterium
Vacuole
Antigens of the
bacterium are placed
in the cell surface The B cell is activated, and divides to produce
membrane plasma cells which secrete antibodies, or
Antigens memory cells which remain in the blood
Plasma cell
Antibodies
Figure 11.2 The response of B-lymphocytes to antigens

Action of T-lymphocytes
11 Immunity
T-lymphocytes include T helper cells and T killer cells. Both of these types
of cell place their specific receptors in their cell surface membranes. On
encountering the relevant antigen, they are activated and divide by mitosis to
form a clone.
Activated T helper cells secrete chemicals called cytokines. These stimulate
B-lymphocytes to produce plasma cells, and stimulate monocytes and
macrophages to attack and destroy pathogens.
Activated T killer cells attach to body cells that display the antigen matching
their receptor. This happens when a virus invades a body cell. The T killer cell
destroys the infected body cell (Figure 11.3).
Some of the clone of T cells become memory cells, which remain in the body
and can react swiftly if the same pathogen invades again.
Antigen-presenting T helper or T killer
cell ingests an cell displaying
antigen receptor specific
to antigen
Antigens are The T cell meets

placed in the its specific antigen
cell surface
membrane
T killer cells The T cell becomes

activated, and divides to
produce T helper cells, T killer
cells (depending on the
nature of the original T cell)
or T memory cells
A T killer cell meets its

specific antigen on the T helper cells secrete
surface of an infected cell cytokines to stimulate
phagocytic cells and Revision activity
Infected B lymphocytes
cell l On a large sheet of paper, make
diagrams of a monocyte, a
neutrophil, a B-lymphocyte and a
The T killer cell kills the T-lymphocyte. Annotate each one
cell it attaches to (or use each one as the centre of a
spider diagram) to explain what it
does..
Figure 11.3 The response of T-lymphocytes to antigens
Antibodies and vaccination

Antibodies Revised
Antibodies are glycoproteins called immunoglobulins that are secreted by Typical mistake
plasma cells in response to the presence of antigens (Figure 11.4). Do not confuse antibodies with
The variable region of the immunoglobulin molecule is specific to the particular antibiotics (see p. 68).
clone of B-lymphocytes that secreted it. It is able to bind with a particular type
of antigen molecule. Immunoglobulins can:
l stick bacteria together, making it impossible for them to divide or making it
easier for phagocytes to destroy them
Immunity 71

l neutralise toxins (poisonous chemicals) produced by pathogens
11 Immunity
l prevent bacteria from sticking to body tissues

l bind to viruses and prevent them infecting cells
Variable region of the four polypeptides;

Light polypeptide chain this is the part that binds to the antigen
Disulfide bonds
Heavy polypeptide chain
Figure 11.4 An immunoglobulin molecule
Immunity Revised
A person is immune to a disease if the pathogen that causes the disease is

unable to reproduce in the body and cause illness. This happens when the body
already contains, or is able rapidly to make, large quantities of antibodies against
the antigens associated with the pathogen.
Active immunity occurs when the person’s own lymphocytes make the
antibody. This could be natural, as a result of the person having previously had
the disease and forming B or T memory cells. It could also be artificial, as a
result of vaccination. This involves introducing weakened pathogens into the
body. The lymphocytes react to the antigens on the pathogens by producing
antibodies and memory cells.
Passive immunity occurs when antibodies from elsewhere are introduced into Revision activity
the body. In a young baby this can be natural, as the baby acquires antibodies l Construct a table to compare the
from its mother in breast milk. It can also be artificial, as the result of an features of natural active immunity,
injection of antibodies obtained from another animal. artificial active immunity, natural
passive immunity and artificial
Active immunity lasts much longer than passive immunity, because memory passive immunity. Your table
cells last a long time, whereas individual antibodies do not. Injections of should include how they are
antibodies, however, can be useful if a person requires instant immunity, for acquired, what is happening in the
body, and how long the immunity
example if an aid worker is about to travel to an environment where risk of a lasts.
disease such as hepatitis is high.
Global eradication of infectious disease Revised
The World Health Organization has helped to organise worldwide campaigns to

eliminate the serious infectious diseases smallpox and poliomyelitis.
Smallpox has been successfully eradicated by vaccinating large numbers of
children with weakened viruses similar to those that cause smallpox. This
succeeded because the vaccine is highly effective. The programme involved the
vaccination of all relatives and contacts of anyone who had the disease — this is
called ring vaccination. The virus did not mutate, so the same vaccine could be
used everywhere.
Diseases that have not been successfully eradicated include the following:
l Measles. This is partly because several successive doses of vaccine are
required to produce immunity, especially in children who have weak
immune systems owing to poor diet or living conditions. The virus is highly
infectious, so a very high percentage of people must be vaccinated to ensure
a population is free of it. Booster vaccinations are also needed. This is difficult
to achieve in places where infrastructure is poor.
l TB. The BCG vaccination gives only partial immunity, although new vaccines
are now being developed, which it is hoped will be more effective. TB is

difficult to treat because the bacteria live inside body cells. Many strains have
11 Immunity
developed resistance to antibiotics.
l Malaria. No effective vaccine has yet been developed against Plasmodium.
This is a eukaryotic organism, not a bacterium or virus, and is not affected by
T-lymphocytes or by antibodies produced by B-lymphocytes.
l Cholera. This disease is caused by the bacterium Vibrio cholerae. In the
body, it lives and reproduces in the intestine, which is outside the body
tissues and not easily reachable by lymphocytes or antibodies. Current
cholera vaccines are ineffective, partly because injected vaccines do not
readily reach the intestines. Oral vaccines are being developed, which are
proving more effective.
Vaccines are, of course, completely ineffective against any diseases that are not
caused by pathogens, such as sickle cell anaemia.
Monoclonal antibodies Revised
Monoclonal antibodies are large quantities of identical antibodies produced

by a clone of genetically identical plasma cells. They have many uses in medicine
(see below).
Producing monoclonal antibodies

Plasma cells are not able to divide, so it is not possible to make a clone of them. A
plasma cell is therefore fused with a cancer cell, to produce a hybridoma cell. This
cell retains the ability of the plasma cell to secrete a particular antibody, and also
the ability of the cancer cell to divide repeatedly to produce a clone (Figure 11.5).
1 A mouse or other organism is injected with an antigen that will stimulate the
production of the desired antibody.
2 B-lymphocytes in the mouse that recognise this antigen divide to form a
clone of plasma cells able to secrete an antibody against it.
3 B-lymphocytes are then taken from the spleen of the mouse. These are
fused with cancer cells to produce hybridoma cells.
4 To identify the hybridoma cells that produce the desired antibody, individual
cells are separated out. Each hybridoma cell is tested to see if it produces
antibody on exposure to the antigen. Any that do are cultured so that they
produce a clone of cells all secreting the desired antibody.
A mouse is injected with an antigen. Plasma cells from the spleen
of the mouse are mixed with
cancer cells.
Many very tiny samples

are taken, so there is only
one cell in each sample.
Every sample is tested to
find a hybridoma cell – the
product of the fusion of a
mouse plasma cell producing The sample containing the hybridoma cell
antibody to the antigen and is cloned. Being a cancer cell, it grows in
a cancer cell. unlimited quantities in the laboratory.
Figure 11.5 How monoclonal antibodies are produced
Uses of monoclonal antibodies

Monoclonal antibodies can be used in the diagnosis of disease, and in pregnancy
testing.
Their big advantage is that the tests can be carried out quickly, often producing
results in minutes or hours.
Immunity 73

Diagnosing disease
11 Immunity
Monoclonal antibodies can be used in an ELISA test to diagnose an infectious
disease, by detecting the presence of particular antigens in the blood (Figure
11.6). ELISA stands for enzyme-linked immunosorbent assay.
1 The monoclonal antibody is immobilised on the surface of a small container,
such as a small glass well. The liquid to be tested (for example, blood
plasma) is added to the well. If the antigens are present, they will bind to the
antibodies.
2 The contents of the well are then rinsed out. The antigens stay tightly bound
to the antibodies.
3 Now another solution containing the same monoclonal antibodies is added
to the well. These antibodies also have a reporter enzyme combined with
them. They bind with the antigens already attached to the antibodies in the
well. The well is again rinsed out, so the enzymes will all be washed away
unless they have bound with the antigens.
4 The substrate of the enzyme is then added. If the enzyme is present —
which is only the case if the antigen being investigated was present — then
the substrate is changed to a coloured substance by the enzyme.
The colour change therefore indicates the presence of the antigen in the fluid
being tested.
1 2 3 4
Monoclonal Antigen Enzyme Substrate changed
antibody to coloured
Monoclonal substance due to
antibody presence of enzyme
Figure 11.6 How an ELISA test works
Pregnancy testing
The urine of a pregnant woman contains the hormone human chorionic
gonadotrophin, hCG. Monoclonal antibodies can be used to detect its presence
in urine.
There are many different types of pregnancy testing kits. One type uses a plastic
strip containing three bands:
l The R band contains immobilised monoclonal antibodies that can bind with
hCG. These antibodies have been combined with an enzyme.
l The T band contains more antibodies that can bind with hCG, and also
coloured substrates for the enzymes in the R band.
l The C band is used to check that the strip is working.
When the end of the strip is dipped into urine, the urine moves up the strip by
capillary action. If it contains hCG, this binds with the monoclonal antibodies in
the R band. The complexes of hCG, antibodies and enzymes break free from the
strip, and continue moving up it as the urine seeps upwards.
When they reach the T band, the enzymes attached to the antibodies cause
the substrate to react, producing a coloured product. This produces a coloured
stripe on the test strip.
Treating disease
Monoclonal antibodies can be produced that will bind with particular proteins
on the surface of body cells. For example, the monoclonal antibody rituximab
binds with a protein called CD20, which is found only on B lymphocytes.
This can be useful in the treatment of a type of cancer called non-Hodgkin
lymphoma, in which B cells divide uncontrollably. When rituximab binds to the
B cells, it makes them ‘visible’ to the immune system, which destroys them. New
B cells are made in the bone marrow, and these replacement cells may not be
cancerous.

AS experimental skills and
investigations
Skills and mark allocations

Almost one quarter of the total marks for your AS examination are for
experimental skills and investigations. These are assessed through a practical
examination.
There is a total of 40 marks available for the practical examination. Although the
questions are different each time, the number of marks assigned to each skill is
always the same. This is shown in the table below.
Total
Skill marks Breakdown of marks
Manipulation, 16 Successfully collecting data and 8 marks
measurement and observations
observation, MMO Making decisions about 8 marks
measurements or observations
Presentation 12 Recording data and observations 4 marks
of data and Displaying calculations and reasoning 2 marks
observations, PDO
Data layout 6 marks
Analysis, 12 Interpreting data or observations and 6 marks
conclusions and identifying sources of error
evaluation, ACE Drawing conclusions 3 marks
Suggesting improvements 3 marks
The syllabus explains each of these skills in detail, and it is important that you
read the appropriate pages in the syllabus so that you know what each skill is,
and what you will be tested on.
The next few pages explain what you can do to make sure you get as many
marks as possible for each of these skills. They give you guidance in how you can
build up your skills as you do practical work during your course, and also how
to do well in the examination itself. They are not arranged in the same order as
in the syllabus, or in the table above. Instead, they have been arranged by the
kind of task you will be asked to do, either in practical work during your biology
course or in the examination.
There is a great deal of information for you to take in, and skills for you to
develop. The only way to do this really successfully is to do lots of practical
work, and gradually build up your skills bit by bit. Don’t worry if you don’t get
everything right first time. Just take note of what you can do to improve next
time — you will steadily get better and better.
The practical examination

questions
There are usually two questions in the practical examination. The examiners will
take care to set questions that are not exactly the same as any you have done
AS experimental skills and investigations 75

before. It is possible that there could be three shorter questions instead of two
longer ones, so do not be surprised if that happens.

It is important that you do exactly what the question asks you to do. Students
often lose marks by doing something they have already practised, rather than
doing what the question actually requires. Expert tip
During your course:
Question 1 is likely to be what is sometimes called a ‘wet practical’. For example, l Every time you do a practical
it could be: during your AS course, time
l an investigation into the activity of an enzyme yourself. Are you working quickly
enough? You will probably find
l an osmosis experiment
that you are very slow to begin
l tests for biological molecules with, but as the course progresses
try to work a little faster as your
This question will often ask you to investigate the effect of one factor on confidence improves.
another — for example, the effect of enzyme concentration on rate of reaction,
In the exam:
or the effect of leaf area on the rate of transpiration. l Do exactly what the question asks
Question 2 is likely to involve making drawings from a specimen. This could you to do. This is unlikely to be
exactly the same as anything you
be a real specimen, or it could be a photograph. You may be asked to use a have done before.
microscope, a stage micrometer and eyepiece graticule, or images of them, to l Leave yourself enough time to
work out the magnification or size of the specimen. do each question, spending an
appropriate number of minutes on
The two questions are designed to take approximately equal amounts of your each one.
time. You should therefore aim to spend about 1 hour on each question.
How to do well in the

practical exam
Variables Revised
Many of the experiments that you will do during your AS course, and usually
Question 1 in the examination paper, will investigate the effect of one factor on The independent variable is
another. These factors are called variables. the factor that you change in an
The factor that you change or select is called the independent variable. The investigation.
factor that is affected (and that you measure when you collect your results) is The dependent variable is the factor
the dependent variable. The table below shows some examples. that changes as the result of changes in
the independent variable.
Independent Dependent
Investigation variable variable
1 Investigation into the effect Temperature Volume of oxygen
of temperature on the rate of produced per
breakdown of hydrogen peroxide by minute
catalase
2 Investigating the effect of immersion Sucrose Change in length
in solutions of different sucrose concentration of potato strip
concentration on the change in
length of potato strips
3 Testing the hypothesis: the density Upper or lower Number of
of stomata on the lower surface of surface of the stomata per cm3
a leaf is greater than the density on leaf
the upper surface
4 Investigation into the effect of leaf Total area of Rate of movement
area on transpiration rate leaves of meniscus
We will keep referring back to these four examples in the next few pages, so you
might like to put a marker on this page so you can easily flip back to look at the
table as you read.

If you are investigating the effect of one variable on another, then you need to

be sure that there are no other variables that might be affecting the results. It is
important to identify these and — if possible — keep them constant. These are
sometimes called control variables.
Making decisions about the independent variable

You may have to make your own decisions about the range and interval of the
independent variable.
Let’s think about Investigation 1 in the table above — investigating the effect of
temperature on the rate of breakdown of hydrogen peroxide by catalase.
The independent variable is the temperature. First, decide on the range of
temperatures you will use. The range is the spread between the highest and
lowest value. This will be affected by:
l the apparatus you have available to you, which will determine the possible
range of temperatures you can produce. In this case, you will probably
be using a water bath. If you are lucky, you may have a thermostatically
controlled water bath, but in the exam you will probably have to use a
beaker of water whose temperature you can control by adding ice or by
heating it.
Gas syringe
Reacting mixture
Heat
Changing the independent variable
l your knowledge about the range of temperature over which the rate of
activity of the enzyme is likely to be affected. Even if you could manage it,
there would not be much point in trying temperatures as low as −50 °C or as
high as 200 °C. However, you probably know that various enzymes can have
optimum temperatures anywhere between 20 °C and 80 °C, so you should
include these values in the range.
Next, decide on the intervals that you will use. The interval is the distance
between the values that you choose. This will be affected by:
l the number of different values you can fit in within your chosen range, and
how much time you have available to you. For example, you might ideally
like to use intervals of 5 °C, so that you set up water baths at 0 °C, 5 °C and so
on, all the way up to 80 °C. But obviously that would not be sensible if you
only have five water baths, or if you only have 1 hour to do the experiment.
l the number of results you need to obtain. You are going to be looking
for any pattern in the relationship between the independent variable
(temperature) and the dependent variable (rate of reaction). You will need
at least five readings to see any pattern. There is really no point trying to
draw a graph if there will be fewer than five points on it. So, if your range of
temperatures is 0 °C to 80 °C, you could use intervals of 20 °C. This would
give you five readings — 0, 20, 40, 60 and 80 °C.

Producing different concentrations of a solution
In Investigation 2, Investigating the effect of immersion in solutions of different
sucrose concentration on the change in length of potato strips, the independent
variable is the concentration of a solution. You may be given a sucrose solution
of a particular concentration, and then be asked to produce a suitable range of
concentrations to carry out the experiment.
The range you should use will usually be from 0 (distilled water) up to the
concentration of the solution you have been given (because obviously you
cannot easily make that into a more concentrated solution).
The intervals you use could be either:
l all the same distance apart, for example concentrations of 0.8, 0.6, 0.4 and
0.2 mol dm−3 (and, of course, 0.0 mol dm−3)
l produced by using serial dilutions to make concentrations of 0.1, 0.01 and
0.001 mol dm−3 (and, of course, 0.0 mol dm−3)

How to… Produce a range of solutions of different concentrations from one given concentration
Let’s say you need 10 cm3 of sucrose solution of each concentration.

Producing a range with equal intervals
Take a particular volume of your original solution and place it in a clean tube. Add distilled water to make it up to 10 cm3 .
Then do the same again, using a different volume of the original solution.
The table below gives some examples.
Producing a range of concentrations with equal intervals from a 1.0 mol dm−3 solution
Volume taken of original Volume of distilled water Concentration of solution produced/

1 mol dm−3 solution/cm3 added/cm3 mol dm−3
10 0 1.0
8 2 0.8
6 4 0.6
4 6 0.4
2 8 0.2
0 10 0.0
Producing a range using serial dilutions

You could be asked to make up a series of solutions in which each one has a concentration that is one tenth of the previous one.
Take 1 cm3 of your original solution and place it in a clean tube.
Add distilled water to make it up to 10 cm3 .
Now mix this new solution really well, and then take 1 cm3 of it. Put this into a clean tube and make it up to 10 cm3 .
Keep doing this, each time taking 1 cm3 from the new solution.
The table below summarises this.
Producing a range of concentrations using serial dilution of a 1.0 mol dm−3 solution
Solution used/ Volume taken of Volume of distilled Concentration of solution

mol dm−3 solution/cm3 water added/cm3 produced/ mol dm−3
1.0 10 0 1.0
1.0 1 9 0.1
0.1 1 9 0.01
0.01 1 9 0.001
0.001 1 9 0.0001
0.0001 1 9 0.00001
You could also be asked to make up solutions where each is one half of the concentration of the previous solution.

Continuous and discontinuous variables
In Investigation 1, the independent variable (temperature) is continuous. This
A continuous variable can have any
means that we can choose any value within the range we have decided to use.
value within its range.
This is also true for Investigation 2, where we can choose any value of
A discontinuous variable has only a
concentration within the range we have decided to use.
limited number of possible values.
Sometimes, however, the independent variable is discontinuous. This means
that there is only a limited number of possible values. For example, in
Investigation 3, Testing the hypothesis: the density of stomata on the lower Expert tip
surface of a leaf is greater than the density on the upper surface, the During your course:
independent variable has only two possible ‘values’ — either the upper surface l Every time you do an experiment,
of the leaf, or the lower surface of the leaf. So you don’t have any choice about identify and write down the
the range or intervals at all! independent variable and the
dependent variable.
Controlling the control variables l Every time you do an experiment,
In your experiment, it is important to try to make sure that the only variable think about the range and the
intervals of the values you are using
that could be affecting the dependent variable is the independent variable that for the independent variable. For
you are investigating. If you think there are any other variables that might affect your own benefit, write down what
it, then you must try to keep these constant. the range is and what the intervals
are, just to help you to think about
Look back at the table on p. 76. them.
In Investigation 1, the important control variables would be the concentration l Learn how to make up dilutions
from a solution of a given
and volume of the enzyme solution and the concentration and volume of the concentration, and practise doing
hydrogen peroxide solution. Changes in any of these would have a direct effect this until you feel really confident
on the rate of reaction. about it.
In Investigation 2, the important control variables would be the dimensions of In the exam:
l Read the question carefully, then
the potato strips and the potato tuber from which they came. You also need to identify the independent variable
think about time, but here the important thing is that the strips are left in the and the dependent variable (even
solution for long enough for equilibrium to be reached — after that, it doesn’t if the question does not ask you to
really matter if one is left for slightly longer than another. You also need to be do this).
sure that all the strips are completely immersed in the solution, although the l Next, decide if the independent
variable is continuous or
actual volume of the solution doesn’t matter. Temperature, too, will not affect
discontinuous (see above).
the final result, but it could affect the speed at which equilibrium is reached l If it is continuous, read the
— if you leave the strips for long enough, then it does not really matter if the question carefully to see if you
temperature varies. have been told the range and
intervals to use, or if you are being
In biology, we often want to do experiments where it is not possible to control asked to decide these for yourself.
all the variables. For example, we might want to investigate the effect of body
mass index on heart rate when at rest. There are all sorts of other variables that
might affect resting heart rate, such as gender, age, fitness, when a person last Expert tip
ate and so on. In this case, we just have to do the best we can, for example, by During your course:
limiting our survey to males between the ages of 20 and 25. If we can collect l Every time you do an experiment,
results from a large random sample among this group of males, then we can think about which variables you
hope that at least we will be able to see if there appears to be a relationship have been told to control, or make
between our independent and dependent variables. your own decision about which
ones are important to control.
When to measure the dependent variable Get to know the standard ways
of controlling variables such as
In many experiments you will need to decide when, and how often, you should
temperature (use water baths), pH
take a reading, observation or measurement of the dependent variable. (use buffer solutions) and other
l With some investigations, you will need to leave things long enough for variables.
whatever is happening to finish happening. This would be important in In the exam:
Investigation 2, where you would need to leave the potato strips in the l If you are not told what variables
sucrose solutions for long enough for equilibrium to be reached. to control, then think about these
carefully before deciding what you
will control and how you will do it.

l With some investigations, you may need to begin taking readings straight Expert tip
away. This would be important in Investigation 1, where you should begin During your course:
measuring the volume of oxygen released each minute from time 0, which is l Every time you do an experiment
the moment that the enzyme and its substrate are mixed. where time is involved, think about
why you should start timing from a
l With some investigations, you may need to allow time for a process to settle
particular moment, and when and
down to a steady rate before you begin to take readings. This would be why you should take readings.
important in Investigation 4, where you would be measuring the rate of In the exam:
transpiration in a particular set of conditions. l Think carefully about whether
or not time is important. If you
think it is, then decide when you
will start taking readings, and
how often you will take them.
Remember that if you are going to
use them to draw a graph, you will
need at least five points to plot.
Taking measurements Revised
You will often be asked to take measurements or readings. In biology, these

are most likely to be length, mass, time, temperature or volume. You could
be taking readings from a linear scale (for example, reading temperature on a
thermometer, reading volume on a pipette, or reading length on a potometer
tube). You could be reading values on a digital display, for example reading mass
on a top pan balance or time on a digital timer.
There are some special terms that are used to describe measurements, and the
amount of trust you can put into them. It is easiest if we think about them in
terms of a particular experiment, so let’s concentrate on Investigation 1. Look
back at p. 76 to remind yourself what is being measured.
Validity This is about whether what you are measuring is what you actually
intend to measure. For example, in Investigation 1, does measuring the volume
of oxygen in the gas syringe each minute really tell you about the rate of
reaction? It is a valid method in this instance, because the volume of oxygen
given off per unit time is directly related to the rate at which the reaction is
taking place.
Reliability This is how well you can trust your measurements. Reliable results
are ones that are repeatable. This could be affected by various factors, such as
whether you are able to take a reading at the precise time you intended to.
Accuracy An accurate reading is a true reading. For your readings of volume to
be accurate, then the gas syringe must have been calibrated correctly, so that
when it says the volume of gas is 8.8 cm3, then there really is exactly 8.8 cm3 of
gas in there.
Precision If you were able to put exactly 8.8 cm3 of gas into your gas syringe,
and it read 8.8 cm3 every time, then your readings have a high degree of
precision. If, however, the syringe did not always read the same value (so there
was variation in its readings, even though the actual volume of gas was exactly
the same), then your measurements are less precise.
Resolution You probably already know this term, because we use it in
microscopy to tell us the degree of detail that we can see. The smaller the detail,
the higher the resolution. It means very much the same thing with a measuring
instrument — the smaller the division on the scale of the measuring instrument,
the higher its resolution. So, for example, a 10 cm3 gas syringe marked off every
0.5 cm3 has a higher resolution than a 20 cm3 gas syringe marked off every 1 cm3.
If you get a choice, then go for the instrument with the highest resolution to
make your measurements — so long as it can cover the range that you need.

Uncertainty in measurements — estimating errors

Whenever you take a reading or make a measurement, there will be some
uncertainty about whether the value is absolutely correct. These uncertainties An experimental error is a factor that
are experimental errors. Every experiment, no matter how well it has been reduces the reliability of your results,
such as limitations of the technique or
designed, no matter how carefully it has been carried out and no matter how
apparatus used, or inability to control a
precise and accurate the measuring instruments, has this type of error.
variable that affects the results. It must
You may be asked to estimate the size of the errors in your measurements. not be confused with a human mistake.
This is nothing to do with how well you have made the measurements — the
examiners do not want to know about ‘mistakes’ that you might have made,
such as misreading a scale or taking a reading at the wrong time. It is all about
the inbuilt limitations in your measuring device and its scale.
l In general, the size of the error is half the value of the smallest division on the
scale. For example, if you have a thermometer that is marked off in values of
1° C, then every reading that you take could be out by 0.5 °C. You can show
this by writing: 21.5 °C ± 0.5 °C.
l If your recorded result involves measuring two values — for example, if you
have measured a starting temperature and then another temperature at the Expert tip
end, and have calculated the rise in temperature — then this error could Every time you take a reading or make
have occurred for both readings. The total error is therefore the sum of the a measurement, get into the habit of
working out and writing down the
errors for each reading. Your final value for the change of temperature you error (uncertainty) in each reading.
have measured would then be written: 18.0 °C ± 1.0 °C.
Recording measurements and other data Revised
You will often need to construct a table in which to record your measurements,
readings and other observations. It is always best to design and construct your
results table before you begin your experiment, so that you can write your
readings directly into it as you take them.
Let’s think about Investigation 2 again. You have made your decisions about the
range and intervals of the independent variable (concentration of solution) — you
have decided to use six concentrations ranging from 0.0 mol dm−3 to 1.0 mol dm−3.
Your dependent variable is the change in length of the potato strips, and you are
going to find this by measuring the initial length and final length of each strip.
These are the things you need to think about when designing your results table:
l The independent variable should be in the first column.
l The readings you take are in the next columns.
l Sometimes, these readings actually are your dependent variable. In this
experiment, however, you are going to have to use these readings to calculate
your dependent variable, which is the change in length of the strips. So you
need to have another column for this. This comes at the end of the table. In
fact, you really need to work out the percentage change in length of the strips,
as this will allow for the inevitable variability in the initial lengths of the strips.
The table could look like this:
Results table for Investigation 2
Concentration of sucrose Initial length of Final length of Change in length of Percentage change in
solution/ mol dm−3 potato strip/mm potato strip/mm potato strip/mm length of potato strip
0
0.2
0.4
0.6
0.8
1.0

Notice:
l The table has been clearly drawn, with lines separating all the different rows
and columns. Always use a pencil and ruler to draw a results table.
l Each column is fully headed, including the unit in which that quantity is
going to be measured. The unit is preceded by a slash (/). You can use
brackets instead, for example, concentration of sucrose solution (mol dm−3).
l The slash always means the same thing. It would be completely wrong to
write: concentration of sucrose solution/mol/dm3 as the heading of the first
column. That would be really confusing. If you are not happy using negative
indices like dm−3, you can always write ‘per’ instead. So it would be fine to
write: concentration of sucrose solution/mol per dm3.
l The columns are all in a sensible order. The first one is the independent
variable, so you can write these values in straight away, as you have already
decided what they will be. The next thing you will measure is the initial
length of the strip, then the final length. Then you will calculate the change
in length, and finally you will calculate the percentage change in length.
So now you are ready to do your experiment and collect your results. Here is
what your table might look like.
Completed results table for Investigation 2
Concentration of sucrose Initial length of Final length of Change in length of Percentage change in
solution/ mol dm−3 potato strip/mm potato strip/mm potato strip/mm length of potato strip
0 49.5 52.0 +2.5 +5.1
0.2 50.0 52.0 +2.0 +4.0
0.4 50.5 51.5 +1.0 +2.0
0.6 50.0 50.5 +0.5 +1.0
0.8 49.0 48.0 −1.0 −2.0
1.0 49.5 48.0 −1.5 −3.0
Notice:
l All the measurements in the second two columns were made to the nearest
0.5 mm. This is because the smallest graduation on the scale on the ruler
was 1 mm, so it was possible to estimate the length to the nearest 0.5 mm.
(Have a look at the scale on your ruler, and you will see that this is sensible.)
Even if you decide that a length is exactly 50 mm, you must write in the next
decimal place for consistency, so you would write 50.0.
l The values in the ‘change in length’ column each show whether they were an
increase or a decrease.
l The percentage change in length is calculated like this:
change in length
× 100
initial length
(Do make sure you remember to take a calculator into the exam with you.)
l Each percentage change in length has been rounded up to one decimal
place, for consistency with the change in length. For example, the calculation
in the first row gives 6.0606, which you should round up to 6.1. The
calculation in the sixth row gives 4.0404, which rounds down to 4.0.
Repeats
It is a good idea to do repeats. This means that, instead of getting just one
reading for each value of your independent variable, you collect two or three.
You can then calculate a mean value, which is more likely to be a ‘true’ value
than any of the individual ones.
Let’s say that you did this for the potato strip experiment. You could have
used two potato strips for each sucrose concentration, then calculated the

percentage change in length for each one, then finally calculated a mean

percentage change.
This means adding some extra rows and an extra column to the results table,
like this:
Completed results table (with repeats) for Investigation 2
Concentration of Initial length Final length Change in length Percentage change Mean percentage
sucrose solution/ of potato of potato of potato strip/ in length of potato change in length of
mol dm−3 strip/mm strip/mm mm strip potato strip
0 49.5 52.0 +2.5 +5.1 +4.6
49.0 51.0 +2.0 +4.1
0.2 50.0 52.0 +2.0 +4.0 +4.0
50.5 52.5 +2.0 +4.0
0.4 50.5 51.5 +1.0 +2.0 +2.5
49.5 51.0 +1.5 +3.0
0.6 50.0 50.5 +0.5 +1.0 +0.5
51.0 51.0 0.0 0.0
0.8 49.0 48.0 −1.0 −2.0 −2.0
50.5 49.5 −1.0 −2.0
1.0 49.5 48.0 −1.5 −3.0 −3.0
50.5 49.0 −1.5 −3.0
Notice:
l All of this information has been put in a single results table. This makes it
much easier for someone to read and find all the information they need.
l The numbers in the final column have again been rounded up to one
decimal place.
Qualitative observations
The results table for the potato strip experiment contains numerical values —
they are quantitative. Sometimes, though, you may want to write descriptions
in your results table, for example a colour that you observed. These are
qualitative observations. If you are recording colours, write down the actual
colour — do not just write ‘no change’.
Use simple language that everyone can easily understand. Avoid using terms
that are difficult for the examiner to interpret, such as ‘yellowish-green’. Think
about what is important — perhaps it is that this tube is a darker or lighter
green than that tube. Using simple language such as ‘dark green’ or ‘a lighter
green than tube 1’ is fine.
Graphs and other ways of displaying data Revised
When you have collected your data and completed your results table, you will
generally want to display the data so that anyone looking at them can see any
patterns.
Line graphs
Line graphs are used when both the independent variable and the dependent
variable are continuous (see p. 79). This is the case for the potato strip data
above. The graph can help you to decide if there is a relationship between the
independent variable and the dependent variable. This is what a line graph of
these data might look like.

Mean 5
percentage
change in
length 4
0
0.2 0.4 0.6 0.8 1.0
Concentration of
–1 sucrose solution/
mol dm–3
–2
–3
Graph of the results of Investigation 2
Notice:
l The independent variable goes on the x-axis, and the dependent variable
goes on the y-axis.
l Each axis is fully labelled with units. You can just copy the headings from the Expert tip
appropriate columns of your results table. During your course:
l Get plenty of practice in drawing
l The scales on each axis should start at or just below your lowest reading, and
graphs, so that it becomes second
go up to or just above your highest reading. Think carefully about whether nature always to choose the
you need to begin at 0 on either of the axes, or if there is no real reason to correct axes, to label them fully
do this. and to choose appropriate scales.
l The scales use as much of the width and height of the graph paper as In the exam:
possible. If you are given a graph grid on the exam paper, the examiners will l Take time to draw your graph axes
have worked out a sensible size for it, so you should find your scales will fit and scales — you may need to try
out two or even three different
comfortably. The greater the width and height you use, the easier it is to see scales before finding the best one.
any patterns in your data once you have plotted them. l Take time to plot the points — and
l The scale on each axis goes up in regular steps. Choose something sensible, then go back and check them.
such as 1s, 2s, 5s or 10s. If you choose anything else, such as 3s, it is practically l Use a sharp HB pencil to draw the
line, taking great care to touch
impossible to read off any intermediate values. Imagine trying to decide
the centre of each cross if you are
where 7.1 is on a scale going up in 3s. joining points with straight lines.
l Each point is plotted very carefully with a neat cross. Do not use just a dot, If you go wrong, rub the line out
as this may not be visible once you have drawn the line. You could, though, completely before starting again.
use a dot with a circle round it. l If you need to draw two lines on
your graph, make sure you label
l A smooth best-fit line has been drawn. This is what biologists do when each one clearly.
they have good reason to believe there is a smooth relationship between
the independent and dependent variables. You know that your individual
points may be a bit off this line (and the fact that the two repeats for each
concentration were not always the same strongly supports this view), so you
can actually have more faith in there being a smooth relationship than you
do in your plots for each point.
Sometimes in biology (it does not often happen in physics or chemistry!) you
might have more trust in your individual points than in any possible smooth
relationship between them. If that is the case, then you do not draw a best-fit
curve. Instead, join the points with a very carefully drawn straight line, like this:

Rate of 6
transpiration/
arbitrary units
5
0
6.00 am 8.00 am 10.00 am 12.00 noon 1.00 pm
Time of day
Graph where we are not sure of the pattern in the relationship between the
independent and dependent variables
You may be asked to read off an intermediate value from the graph you have Expert tip
drawn. It is always a good idea to use a ruler to do this — place it vertically to During your course:
read a value on the x-axis, and horizontally to do the same on the y-axis. You l Make sure you know how to read
can draw in faint vertical and horizontal pencil lines along the ruler. This will help off an intermediate value from
you to read the value accurately. a graph accurately, and how to
calculate a gradient.
You could also be asked to work out the gradient of a line on a graph. This is In the exam:
explained on p. 28. l Take time over finding
intermediate values on a graph —
Histograms if you rush it is very easy to read off
A histogram is a graph where there is a continuous variable on the x-axis, and a a value that is not quite correct.
frequency on the y-axis. For example, you might have measured the length of 20
leaves taken from a tree. You could plot the data like this:
Number 6
of leaves
5
0
30 40 50 60 70 80
Leaf length/mm
A frequency histogram
Notice:
l The numbers on the x-axis scale are written on the lines. The first bar
therefore includes all the leaves with a length between 30 and 39 mm. The
next bar includes all the leaves with a length between 40 and 49 mm, and so
on.
l The bars are all the same width.
l The bars are all touching — this is important, because the x-axis scale is
continuous, without any gaps in it.

Bar charts
A bar chart is a graph where the independent variable is made up of a number

of different, discrete categories and the dependent variable is continuous.
For example, the independent variable could be type of fruit juice, and the
dependent variable could be the concentration of glucose in the juice.
Concentration 16
of glucose/g 14
per 100 cm3
12
10
8
6
4
2
0
Passion
fruit
Orange
Apple
Cranberry
Pineapple
Type of fruit
Bar chart showing concentration of glucose in different types of fruit juice
Notice:
l The x-axis has an overall heading (type of fruit), and then each bar also has
its own heading (orange, apple and so on on).
l The y-axis has a normal scale just as you would use on a line graph.
l The bars are all the same width.
l The bars do not touch.
Drawing conclusions and interpreting your data Revised
Once you have collected, tabulated and displayed your results, you can use
them to draw a conclusion.
When you are thinking about a conclusion, look right back to the start of your
experiment where you were told (or you decided) what you were to investigate.
For example:
l In Investigation 1, Investigating the effect of temperature on the rate of
breakdown of hydrogen peroxide by catalase, your conclusion should provide
a statement about the relationship between the two variables.
l In Investigation 2, Investigating the effect of immersion in solutions of
different sucrose concentration on the change in length of potato strips,
your conclusion should state the relationship between the concentration of
sucrose solution and the change in length of the potato strips.
l In Investigation 3, Testing the hypothesis: the density of stomata on the
lower surface of a leaf is greater than the density on the upper surface,
your conclusion should say whether your results support or disprove this
hypothesis.
Explaining your reasoning

There will often be marks for explaining how you have reached your conclusion.
Your reasoning should refer clearly to your results. For example, your conclusion
to Investigation 2 (whose results are shown in the table on p. 83) might be as
follows:
l A sucrose solution with a concentration of 0.6 mol dm−3 and below
caused an increase in length of the potato strips. A sucrose solution with a
concentration of 0.8 mol dm−3 and above caused a decrease in length of the

potato strips. From the graph, the solution that I would expect to cause no

change in length of the strips would be 0.62 mol dm−3.
l The strips gained in length because they took up water, which was
because the water potential of the sucrose solution was greater than the
water potential in the potato cells. This therefore means that the water
potential inside the potato cells was the same as the water potential of a
0.62 mol dm−3 sucrose solution.
Showing your working, and significant figures Expert tip

You may be asked to carry out a calculation and to show your working. There During your course:
will be marks for doing this. If you do not show your working clearly, you will not l Get into the habit of describing
get full marks, even if your answer is absolutely correct. the main steps in your reasoning
when drawing a conclusion, and
For example, imagine you have measured four lengths as 46 mm, 53 mm, 52 mm using evidence from the results to
and 48 mm. You are asked to calculate the mean and to show your working. You support it.
should write this down properly as: l Get into the habit of taking time
to set out all your calculations very
mean length = 46 + 53 + 52 + 48 mm = 50 mm clearly, showing each step in the
4 process.
You have already seen, on p. 82, that the final answer to a calculation should l Get into the habit of giving final
have the same number of significant figures as the original numbers you were numerical answers to calculations
to the same number of significant
working from. If you do the calculation above, you will find the answer you get figures as the readings you took, or
is 49.75. But the original measurements were only to two significant figures (a the values you were given.
whole number of mm) so that is how you should give the final answer to your In the exam:
calculation. You must round the answer up or down to give the same number l Even if you feel rushed, take
of significant figures as the original values from which you are working. time to write down the steps in
calculations and reasoning fully.
There is another example of showing your working on p. 90.
Identifying sources of error Revised
It is worth repeating that it is very important to understand the difference

between experimental errors and ‘mistakes’. A mistake is something that you do
incorrectly, such as misreading the scale on a thermometer, or taking a reading
at the wrong time, or not emptying a graduated pipette fully. Do not refer to
these types of mistake when you are asked to comment on experimental errors.
You have already seen, on p. 81, that every measuring instrument has its own
built-in degree of uncertainty in the values you read from it. You may remember
that, in general, the size of the error is half the value of the smallest division on
the scale.
Errors can also occur if there were uncontrolled variables affecting your results.
For example, if you were doing an investigation into the effect of leaf area on the
rate of transpiration, and the temperature in the laboratory increased while you
were doing your experiment, then you cannot be sure that all the differences in
rate of transpiration were entirely due to differences in leaf area.
Systematic and random errors

Systematic errors are ones that are the same throughout your investigation,
such as intrinsic errors in the measuring instruments you are using.
Random errors are ones that can differ throughout your investigation. For
example, you might be doing an osmosis investigation using potato strips taken
from different parts of a potato, where perhaps the cells in some parts had a
higher water potential than in others. Or perhaps the temperature in the room
is fluctuating up and down.
Spotting the important sources of error

You should be able to distinguish between significant errors and insignificant
ones. For example, a change in room temperature could have a significant

effect on the rate of transpiration (Investigation 4) but it would not have any
Expert tip
effect at all on the number of stomata on the upper and lower surface of a leaf During your course:
(Investigation 3). l Every time you do an investigation,
work out and write down the
Another thing to consider is how well a variable has been controlled. If you were uncertainty in all the types of
doing an enzyme investigation using a water bath to control temperature, then measurement that you make.
you should try to be realistic in estimating how much the temperature might l Every time you do an investigation,
have varied by. If you were using a high-quality, electronically controlled water think carefully about any errors
bath, then it probably did not vary much, but if you were using a beaker and that may be due to lack of control
Bunsen burner then it is likely that temperature variations could indeed be of variables — which ones might
genuinely be significant?
significant.
In the exam:
l If you are asked about an
Suggesting improvements
investigation that seems familiar,
You might be asked to suggest how the investigation you have just done, or an it is tempting just to try to recall
investigation that has been described, could be improved. Your improvements what the main errors were in the
should be aimed at getting more valid or reliable results to the question that the investigation that you did before.
investigation was trying to answer — do not suggest improvements that would This is not a good idea, because the
mean you would now be trying to answer a different question. For example, if investigation in the exam may not
be quite the same. Always think
you were doing an investigation to investigate the effect of leaf area on the rate about the actual investigation in
of transpiration, do not suggest doing something to find out the effect of the the examination question, and
wind speed on the rate of transpiration. think through what the significant
sources of error are.
The improvements you suggest could include controlling certain variables
that were not controlled, or controlling them more effectively. For example,
Expert tip
you might suggest that the investigation could be improved by controlling
temperature. To earn a mark, you must also say how you would control it, for During your course:
l If time allows, try to do at least
example by placing sets of test tubes in a thermostatically controlled water bath. two (and possibly three) repeats
You could also suggest using better methods of measurement. For example, you when you do an investigation.
l As you do an investigation,
might suggest using a colorimeter to measure depth of colour, rather than using
be thinking all the time about
your eyes and a colour scale. how reliable or accurate your
It is almost always a good idea to do several repeats in your investigation and measurements and readings are.
Think about what you would like
then calculate a mean of your results. For example, if you are measuring the to be able to do to improve their
effect of light intensity on the rate of transpiration, then you could take three reliability or accuracy.
sets of readings for the volume of water taken up by your leafy shoot in 1 In the exam:
minute at a particular light intensity. The mean of these results is more likely to l Be very precise in suggesting how
give you the true value of the rate of transpiration than any one individual result. you could improve the investigation
— for example, do not just say you
would control a particular variable,
but say how you would control it.
Drawings Revised
One of the questions in the exam is likely to involve drawing a specimen on a

slide, using a microscope, or drawing from a photomicrograph (a photograph
taken through a microscope).
Making decisions about what to draw

You might have to decide which part of a micrograph to draw. For example,
there might be a micrograph of a leaf epidermis, and you are asked to draw two
guard cells and four epidermal cells. It is really important that you do exactly as
you are asked and choose an appropriate part of the micrograph.
Producing a good drawing

It is very important that you draw what you can see, not what you think you
ought to see. For example, during your AS course you may have drawn a TS of
a stem where the vascular bundles were arranged in a particular way, or were a
particular shape. In the exam, you could be asked to draw a completely different
type of vascular bundle that you have never seen before. Look very carefully and
draw what you can see.

Your drawing should: Expert tip

l be large and drawn using a sharp pencil (preferably HB, which can be easily During your course:
erased if necessary) with no shading, using single, clear lines l Make sure you are familiar with the
appearance of all of the structures
l show the structure or structures in the correct proportions. The examiners
listed in the syllabus that you could
will check that the overall shape and proportions of your drawing match be asked about on the practical
those of the specimen. Don’t worry — you do not need to be a wonderful paper. You need to know the
artist — a simple, clear drawing is all that is required. names and distribution of the
l show only the outlines of tissues if you are asked to draw a low power plan tissues. Look in particular at the
learning outcomes marked with
(LPP). A LPP should not show any individual cells. However, if you are using a [PA] at the beginning.
microscope, you may need to go up to high power to check exactly where l Practise drawing specimens from
the edges of the tissues are. micrographs, getting used to using
your own eyes to see what is really
You may be asked to label your drawing. In that case: there, rather than what you think
l use a pencil to draw label lines to the appropriate structure using a ruler, ought to be there.
ensuring that the end of the label line actually touches the structure you are l Practise using an eyepiece graticule
labelling to help you work out the relative
proportions of different parts that
l make sure that none of your label lines cross each other you are drawing.
l write the labels horizontally l Take every opportunity to
l write the labels outside the drawing itself practise drawing specimens from
micrographs or microscope slides,
Calculating magnification or size and either mark them yourself
using a CIE-style mark scheme, or
The use of a stage micrometer and eyepiece graticule is described on p. 9. You
get your teacher to mark them for
might be asked to do this on Paper 3. you. Find out what you need to do
You could also be given the magnification of an image, and asked to calculate to improve, and keep working at it
until you feel really confident.
the real size of something in the image. Below is an example of the kind of thing
In the exam:
you might be asked to do.
l Take one or two sharp HB pencils,
This micrograph shows some cells from a moss. Notice that the magnification a pencil sharpener, a clean ruler
is given. that measures in mm and a good
eraser.
l Settle down and take time to get
your drawing of the specimen
right.
l Use your eyes first, then your
memory.
Magnification ×300
Let us say you are asked to find the mean width of a cell from the tissue in the
micrograph. There are several steps you need to work through here.
l First, decide how many cells you are going to measure. It is generally sensible
to measure a randomly selected sample of five to ten cells.
l Next, decide which ones you will measure. Choose cells where you can see
the edges as clearly as possible, and where you can see the whole cell. If
cells are evenly distributed, it is best to measure the total width of five cells
in a row. That means you have to make fewer measurements, do fewer
calculations and — better still — it reduces the size of the uncertainty in
your measurements. However, if cells are irregularly shaped or distributed,
you should measure each one individually.
l Once you have decided which five cells to measure, mark this clearly on the
micrograph. It does not matter exactly how you do this — perhaps you could
carefully use a ruler to draw a line across the five cells, beginning and ending
exactly at the first edge of the first cell, and the last edge of the fifth cell.

l Now measure the length of the line in mm and write it down.
l Next, calculate the mean length of one cell. Show clearly how you did this.
l Next, convert this length in mm to a length in µm. (Alternatively, you could
do this right at the end of the calculation.)
l Next, use the magnification you have been given to convert this mean length
of the image to a mean real length. Here is what your answer might look like:
total width of 5 cells on micrograph = 29 mm

Therefore,
mean width = 29 mm = 6 mm
5
= 6 × 1000 = 6000 µm
magnification = × 300
Therefore, real mean width of a cell = 6000 µm = 20 µm
300
Making comparisons Revised
You might be asked to compare the appearance of two biological specimens or

structures. You could be observing these using the naked eye or a lens, or using
a microscope, or you could be looking at two micrographs.
The best way to set out a comparison is to use a table. It will generally have
three columns, one for the feature to be compared, and then one for each of
the specimens.
For example, you might be asked to observe two leaves and record the
differences between them. Your table and the first three differences might look
like this:
Feature Leaf A Leaf B

Leaf margin Smooth Toothed
Veins Parallel to each other A central vein with branches
coming off it, forming a network
Shape Length is more than twice Length is less than twice the
the maximum width maximum width
Notice:
l The table has been drawn with ruled lines separating the columns and rows.
l The descriptions of a particular feature for each specimen are opposite one
another (that is, they are in the same row).
l Each description says something positive. For example, in the first row, it
would not be good to write ‘not toothed’ for Leaf A, as that does not tell us
anything positive about the leaf margin.
Note that the practical examination is likely to ask you to describe or compare
observable features, not functions. Do not waste time describing functions when
this is not asked for.

AS exam-style questions
and answers
This practice paper comprises structured questions similar to
those you will meet in the exam. Exemplar paper
You have 1 hour and 15 minutes to do the paper. There are
60 marks on the paper, so you can spend just over 1 minute
per mark. If you find you are spending too long on one Question 1
question, then move on to another that you can answer
more quickly. If you have time at the end, then come back (a) The diagram shows a small part of a cell, as seen
to the difficult one. using an electron microscope.
Some of the questions require you to recall information A B C D
that you have learned. Be guided by the number of
marks awarded to suggest how much detail you should
give in your answer. The more marks there are, the more
information you need to give.
Some of the questions require you to use your knowledge
and understanding in new situations. If you come across
something you have not seen before, just think carefully
about it and find something that you do know that will help
you to answer it.
The best answers are short and relevant — if you target
your answer well, you can get a lot of marks for a very small (i) Name the parts labelled A to D.
amount of writing. Don’t say the same thing several times (2 marks)
over, or wander off into answers that have nothing to do (ii) Describe how part B is involved in the
with the question. As a general rule, there will be twice as formation of extracellular enzymes.
many answer lines as marks. So you should try to answer a (3 marks)
3-mark question in no more than 6 lines of writing. If you are (b) Give two reasons, other than the presence of
writing much more than that, you almost certainly have not part B, why the cell in the diagram cannot be a
focused your answer tightly enough. prokaryotic cell. (2 marks)
Look carefully at exactly what each question wants you to (Total: 7 marks)
do. For example, if it asks you to ‘Explain’, then you need to
say how or why something happens, not just describe what Student A
happens. Many students lose large numbers of marks by not (a) (i) A plasma membrane ✓
reading the question carefully. B Golgi ✓
C nucleus 7
Following each question in this practice paper, there is an
D phagocyte 7
answer that might get a C or D grade, followed by expert
comments (shown by the icon ). Then there is an answer
C is the nuclear envelope (or membrane), not the
that might get an A or B grade, again followed by expert
nucleus itself. A phagocyte is a cell — perhaps the
comments. Try answering the questions yourself before student is thinking of a phagocytic vesicle. Mark: 1/2
looking at these.
Notice that there are sometimes more ticks on the answers
than the number of marks awarded. This could be because
you need two correct responses for 1 mark (e.g. Q1 (a) (i)) or
because there are more potential mark points than the total
number of marks available (e.g. Q1 (a) (ii)). Even if you get
four or five ticks for a 3-mark question, you cannot get more
than the maximum 3 marks.
AS exam-style questions and answers 91

Question 2
AS exam-style questions and answers
Student A
(ii) First, the enzymes are made by protein synthesis on
the ribosomes. Then they go into the endoplasmic The diagram shows the bacterium Mycobacterium
reticulum. Then they are taken ✓ to the Golgi tuberculosis, which causes tuberculosis (TB).
where they are packaged. Then they go in
vesicles ✓ to the cell membrane where they are Plasma/cell surface membrane
sent out by exocytosis.
This student has not really thought about exactly what

the question was asking, and has wasted time writing
about events that take place before and after the involvement
of the Golgi body. There is, however, a mark for the idea that Cell wall Circular DNA
the Golgi body receives proteins that have been in the RER,
2 µm
and another for packaging them into vesicles. Mark: 2/3
(a) M. tuberculosis is taken up by macrophages
Student A and multiplies inside them. Suggest how this
(b) It has a nucleus ✓. And it has Golgi body 7. strategy could help to protect M. tuberculosis
from the immune response by B cells. (3 marks)
The Golgi body is part B, and this has been excluded by (b) In an experiment to investigate how
the question. Mark: 1/2 M. tuberculosis avoids destruction by
macrophages, bacteria were added to a culture
Student B of macrophages obtained from the alveoli of
(a) (i) A cell surface membrane ✓ mice. At the same time, a quantity of small
B Golgi body ✓ glass beads, equivalent in size to the bacteria,
C nuclear envelope ✓ were added to the culture. The experiment was
D vesicle ✓ repeated using increasing quantities of bacteria
and glass beads.
All correct. Mark: 2/2 After 4 hours, the macrophages were sampled
to find out how many had taken up either glass
beads or bacteria. The results are shown in
Student B the graph. The x-axis shows the initial ratio of
(ii) Proteins made in the RER are transported to the bacteria or glass beads to macrophages in the
convex face ✓ of the Golgi body in vesicles. The mixture. Discuss what these results suggest
vesicles fuse ✓ with the Golgi and the proteins about the ability of macrophages to take up M.
inside are modiﬁed ✓ by adding sugars to tuberculosis. (3 marks)
make glycoproteins ✓. They are packaged inside
membranes ✓ and sent to the cell membrane. Percentage 100
of Beads
macrophages
All correct. Mark: 3/3 that took up 75
the particles
50
Student B Bacteria
(b) If it was a prokaryotic cell it would not have a nucleus ✓ 25
and it would have a cell wall ✓.
0
Correct. Mark: 2/2 0.01 0.1 10
1
Particle:macrophage ratio

This is a good answer, which does attempt to ‘discuss’

(c) When M. tuberculosis is present inside a by providing statements about the relatively low ability
phagosome of a macrophage, it secretes of the macrophages to take up the bacteria, but also stating
glycolipids that accumulate in lysosomes that they do take them up. In general, it is always a good idea
and prevent the lysosomes fusing with the to quote data where they are relevant in your answer. Mark:
phagosome. 3/3
Explain how this prevents the macrophage from
destroying the bacterium. (3 marks) Student B
(c) Normally, lysosomes fuse with phagosomes and release
(Total: 9 marks)
hydrolytic enzymes ✓ into them. These enzymes then
hydrolyse (digest) whatever is in the phagosome ✓. If this
Student A does not happen, then the bacteria can live inside the
(a) It stops the B cells seeing them, so they do not make phagosome ✓ without being digested.
antibodies ✓ against them.
All correct. Mark: 3/3
This is not a clear answer. B cells do not ‘see’, so this is
not a good term to use. The ‘they’ in the second half of
the sentence could refer to either B cells or the bacteria. Question 3
Mark: 1/3
Student A (a) The diagrams show a cell in various stages

(b) The macrophages took up more glass beads than of the mitotic cell cycle. Name the stage
bacteria ✓. So they are not very good at taking up the represented by each diagram, and arrange them
bacteria ✓. in the correct sequence. (3 marks)
A B
Just enough for 2 marks, although the second sentence
is weak. Mark: 2/3
Student A
(c) Lysosomes contain digestive enzymes ✓, so if they do
not fuse with the phagosome the bacteria will not get
digested ✓.
C D
Once again, this is the right idea, but not enough

biological detail is given. Mark: 2/3
Student B
(a) B cells only become active when they meet the specific
antigen ✓ to which they are able to respond. If the bacteria (b) Describe the role of spindle microtubules in
are inside a macrophage, then the B cells’ receptors will not mitosis. (3 marks)
meet the antigen ✓ on the bacteria. This means that the B (c) The graph overleaf shows the changes in the
cells will not divide to produce plasma cells ✓, and will not mass of DNA per cell and total cell mass during
secrete antibodies ✓ against the bacteria. two cell cycles. Different vertical scales are used
for the two lines.
This is a good answer. Mark: 3/3
Student B
(b) The cells only started to take up any bacteria when the
particle:macrophage ratio was 1 ✓. On the other hand, they
took up glass beads even when the ratio was above 0.01 ✓.
When the ratio of particles to macrophages was 10, only
about 30% ✓ of the macrophages had taken up bacteria,
whereas over 75% of them had taken up glass beads ✓. This
shows the macrophages do take up the bacteria, but not as
well as they take up glass beads ✓.

Student A
Mass
(d) Mitosis is used in growth and repair. ✓
This is correct, but not a good enough answer for more

than 1 mark at AS. Mark: 1/3
Student B
(a) B prophase ✓, A metaphase ✓, D anaphase ✓,
C telophase ✓✓
0 12 24 36 48 All identified correctly, and in the right order. Mark: 3/3

Key Time/h
Total cell mass
Mass of DNA per cell Student B
(b) Spindle microtubules are made by the centrioles. They
(i) On the graph, write the letter D to indicate latch on to the centromeres ✓ of the chromosomes and
a time at which DNA replication is taking help them line up on the equator ✓. Then they pull ✓ on
place. (1 mark) the centromeres so they come apart and they pull the
(ii) On the graph, write the letter C to indicate chromatids ✓ to opposite ends of the cell in anaphase.
a time at which cytokinesis is taking place.
(1 mark) This is a good answer. Mark: 3/3
(d) Describe the roles of mitosis in living organisms.
(3 marks)
Student B
(Total: 11 marks)
(c) Mass
Student A C✓
(a) A metaphase ✓, B prophase ✓, C telophase ✓,
D anaphase ✓
Each stage is named correctly, but they are not D✓

arranged in the correct order. Mark: 2/3
Student A
0 12 24 36 48
(b) The spindle microtubules pull the chromatids to opposite
Time/h
ends of the cell ✓.
This is correct, but there is not enough here for Both correct. Mark: 2/2
3 marks. Mark: 1/3
Student A Student B
(c) Mass (d) Mitosis produces two daughter cells that are genetically
identical ✓ to the parent cell. Mitosis is used for growth, or
for repairing cells 7. It is also used in asexual reproduction ✓.
C ✓
The point about producing genetically identical cells is a

good one, and it is also correct that mitosis is involved
in asexual reproduction. However, the second sentence
contains an important error. Mitosis cannot repair cells.
D✗ Mitosis can produce new cells, which can help to repair
tissues. Mark: 2/3
0 12 24 36 48
Time/h
Cytokinesis is identified correctly, but DNA replication is

not. The student has written D before the DNA has
replicated. Mark: 1/2

Question 4

Student A
(a) (i) A 7
The diagrams below show five molecules found in
living organisms. A is a glucose molecule, but plants transport sucrose.
Even if you did not know what a sucrose molecule looks
A CH2OH like, you should know that it is a disaccharide.
C O Student A
H H
H
C C (ii) C ✓
OH H
HO OH
C C
Correct. Mark: 1/1
H OH
B CH2OH
Student A
C O
H H HOCH2 O H (iii) E 7
H
C C C C
OH H H HO
HO CH2OH Amino acids are soluble. Either C or D would be correct.
C C O C C
H OH OH H
Student A
C (iv) E ✓
Correct. Mark: 1/1
D Student A
CH2 CH2 CH2 CH2 COO CH2
(v) D ✓
CH2 CH2 CH2 CH2 COO CH
Correct. Mark: 1/1
CH2 CH2 CH2 CH2 COO CH2
E Student A
CH2
(b) Water has dipoles and hydrogen bonds ✓, which help it to
H 2N C COOH dissolve other substances.
CH2 There are no wrong statements in this answer, but it

does not really give an explanation of why water is a
(a) Give the letter of one molecule that fits each good solvent — it just states two facts about water
of these descriptions. You can use each letter molecules. Mark: 1/3
once, more than once or not at all.
(i) the form in which carbohydrates are Student B
transported through phloem tissue in (a) (i) B ✓
plants (1 mark)
(ii) the form in which carbohydrates are stored Correct. Mark: 1/1
in animals (1 mark)
(iii) a molecule that is insoluble in water
(1 mark) Student B
(iv) a molecule that links together with others (ii) C ✓
to form a polypeptide (1 mark)
(v) a molecule that contains ester bonds Correct. Mark: 1/1
(1 mark)
(b) Explain how the structure of water molecules
makes water a good solvent. (3 marks) Student B
(Total: 8 marks) (iii) D or C ✓

Correct. However, the student took an unnecessary risk
with (iii), by giving two answers. If the second one had (b) Describe and explain two ways in which the
been wrong, it could have negated the first correct one. If structure of the alveoli, shown in the diagram,
you are asked for one answer, it is best to give only one. enables gas exchange to take place rapidly.
Mark: 1/1 (4 marks)
(c) Explain why large organisms such as mammals
Student B need specialised gas exchange surfaces, whereas
(iv) E ✓ small organisms such as a single-celled Amoeba
do not. (3 marks)
Correct. Mark: 1/1 (Total: 8 marks)
Student A
Student B
(a) capillary ✓
(v) D ✓
This is correct. Mark: 1/1

Correct. Mark: 1/1
Student A
Student B
(b) They have a large surface area. ✓
(b) In a water molecule, the hydrogen atoms have a tiny
positive electrical charge and the oxygen atom has a similar They are thin, so oxygen can diffuse across quickly. ✓
negative charge ✓. Other atoms or ions with electrical
charges ✓ are attracted ✓ to these charges on the water The statement about a large surface area is correct, but
molecules. This makes them spread about ✓ among the the answer also needs to say why this enables gas
water molecules. exchange to take place rapidly (because the question asks you
to ‘explain’). The second answer is not sufficiently clear —
what is thin? It is not the whole alveoli that are thin, but their
This is a good answer. It really does explain how a
walls. The second part of this answer does give a clear
substance dissolves in water and relates this clearly to
explanation of why this helps gas exchange to take place
the structure of a water molecule. This answer contains four
quickly. Mark: 2/4
possible marking points, but there is a maximum of 3 marks
available in total. Mark: 3/3
Student A
(c) Large organisms have small surface areas compared with
Question 5 their volume ✓, so they need extra surface ✓ to be able to
get enough oxygen.
The diagram below shows a small part of a human
lung as it appears through a microscope. There is a correct and clear statement about surface
area-to-volume ratio, and the answer also just gets a
second mark. However, this is not really very clear — see
answer B for a better explanation. Mark: 2/3
Student B
(a) capillary ✓
Correct. Mark: 1/1
Student B
(b) Large surface area ✓ — so more oxygen and carbon
dioxide molecules can diffuse across at the same time ✓.
Good supply of oxygen — to maintain a diffusion gradient
between the alveoli and the blood.
Wall of alveolus Red blood cell
The first way is correct and well explained. However,
the second, although true, does not answer the
(a) Name the type of blood vessel in which the red
question, which is about the structure of the alveoli.
blood cell is present. (1 mark)
Mark: 2/4

Student B Student A
(c) They have small surface area-to-volume ratios ✓, but an (a) 1 cycle in 0.75 seconds ✓ so in 60 seconds there will be
Amoeba has a large surface area-to-volume ratio. The 60 × 0.75 7 = 45 beats.
oxygen that diffuses in across the surface has to supply
the whole volume ✓ of the animal, so in a large animal The length of one cycle has been read correctly, but the
that is not enough and they have specialised gas exchange calculation is wrong. Mark: 1/2
surfaces to increase the surface area ✓ and let more oxygen
diffuse in. Student A
(b) (i) Left ventricle contracts ✓
This is a good answer. All the important points are there
and are clearly expressed. Mark: 3/3 Pressure/ 16 Semilunar valves
kPa shut ✓
Question 6 12
8
The diagram below shows pressure changes in the
left atrium and left ventricle of the heart and the Right ✗
4 ventricle
aorta during the cardiac cycle.
Pressure/ 16 0
kPa 0 0.2 0.4 0.6 0.8
12 Time/s
8 Correct. Mark: 1/1
4
Student A
(ii) The valves shut when the ventricle starts to relax. ✓
0
0 0.2 0.4 0.6 0.8
Key Time/s This is correct as far as it goes, but more information is
Aorta needed for the second mark. Mark: 1/2
Left ventricle
Student A
Left atrium
(iii) See diagram
(a) Calculate how many heart beats there will be in
1 minute. (2 marks) Correct. Mark: 1/1
(b) (i) On the diagram, indicate the point at
which the semilunar valves in the aorta
snap shut. (1 mark) Student A
(ii) Explain what causes the semilunar valves to (iv) See diagram
shut at this point in the cardiac cycle.
(2 marks) This is partly correct. The pressure in the right ventricle
(iii) On the diagram, indicate the period when is correctly shown as less than that in the left ventricle,
the left ventricle is contracting. but it should be contracting and relaxing at exactly the same
(1 mark) times as the left ventricle. Mark: 1/2
(iv) On the diagram, draw a line to show the
changes in pressure in the right ventricle. Student A
(2 marks) (c) The pressure gets less as the blood gets further away
(c) After the blood leaves the heart, it passes into from the heart ✓. The muscle in the walls of the arteries
the arteries. The blood pressure gradually contracts 7 and relaxes to push the blood along, and it
reduces and becomes more steady as the blood does this in between heart beats so the pulse gets evened
passes through the arteries. out.
Explain what causes this reduction and
steadying of the blood pressure. (2 marks) The first statement is correct, but it does not really tell
(Total: 10 marks) us any more than what is in the question. However, it is
not correct that the muscles in the artery wall contract and
relax to push the blood along. Mark: 1/2

This answer explains very well why the blood pressure
Student B
levels out. However, it does not mention the overall
60 fall in blood pressure. All the same, this is a good answer.
(a) ✓ = 80 beats per minute ✓
0.75 Mark: 2/2
Correct. Mark: 2/2

Question 7
Student B Beetroot cells contain a red pigment that cannot
(b) (i) Left ventricle normally escape from the cells through the cell
✓
Pressure/ 16 contracts surface membrane.
kPa
A student carried out an investigation into the
12 effect of temperature on the permeability of
the cell surface membrane of beetroot cells. She
Semilunar
8 ✓ measured permeability by using a colorimeter
valves shut
to measure the absorbance of green light by the
4 solutions in which samples of beetroot had been
immersed. The greater the absorbance, the more
0 red pigment had leaked out of the beetroot cells
0 0.2 0.4 0.6 0.8 The graph below shows her results.
Time/s
Absorbance of
Correct. Mark: 1/1 green light/
arbitrary units
100
Student B
(ii) They close when the pressure of the blood inside 80
the arteries is higher than inside the ventricles ✓.
The blood therefore pushes down on the valves 60
and makes them shut ✓.
40
Correct. Mark: 2/2
20
Student B 0
(iii) See diagram 0 20 40 60 80
Temperature/ºC
Correct. Mark: 1/1 (a) With reference to the graph, describe the effect
of temperature on the absorbance of light in the
colorimeter. (3 marks)
Student B
(b) With reference to the structure of cell
(iv) See diagram membranes, explain the effects you have
described in (a). (4 marks)
Correct. Mark: 2/2 (Total: 7 marks)
Student B Student A
(c) As the blood is forced into the artery as the ventricle (a) Between 0 and 30 the absorbance goes up very slightly ✓.
contracts ✓, it pushes outwards on the artery wall, making Above 40 °C it goes up very quickly 7. Then it starts to level
the elastic tissue stretch ✓. In between heart beats, the out at about 70 °C ✓.
pressure of the blood inside the artery falls, and the elastic
tissue recoils ✓. So the wall keeps expanding and springing The three main regions of the graph have been
back. When it springs back it pushes on the blood in identified correctly, stating where changes in gradient
between ✓ heart beats, so this levels out the pressure occur. However, the term ‘quickly’ is used, which is not
changes. correct because the graph does not show anything about
time. A third mark could have been gained by quoting some
figures from the graph. Mark: 2/3

Student A
(b) High temperatures damage the proteins in the
membrane ✓. They become denatured, so they leave
holes ✓ in the membrane that the beetroot pigment can
get through.
This is a good answer as far as it goes, and is clearly

expressed. However, it is not enough to score full
marks. Mark: 2/4
Student B
(a) The general trend is that the higher the temperature,
the greater the absorbance. Between 0 and 30 °C, the
absorbance increases very slightly ✓ from about 16 to
18 arbitrary units. Above 40 °C it increases much more
steeply ✓, levelling off at about 70 °C ✓. The maximum
absorbance is 98 arbitrary units.
This is a good answer. However, although some figures

from the graph are quoted, the student has not
manipulated them in any way — for example, the increase in
absorbance between 0 and 30 °C could have been calculated.
Mark: 3/3
Student B
(b) As temperature increases, the phospholipids and protein
molecules in the membrane move about faster ✓ and
with more energy. This leaves gaps in the membrane, so
the beetroot pigment molecules can get through ✓ and
escape from the cell. The protein molecules start to lose
their shape at high temperatures ✓ because their hydrogen
bonds break ✓, so the protein pores get wider ✓, which
increases permeability.
This is an excellent answer. Mark: 4/4

12 Energy and respiration
Energy in living organisms

ATP Revised
ATP stands for adenosine triphosphate. ATP is a phosphorylated nucleotide

— it has a similar structure to the nucleotides that make up RNA. However, it
has three phosphate groups attached to it instead of one (Figure 12.1).
Phosphates
Adenine
Ribose
Figure 12.1 An ATP molecule

ATP is used as the energy currency in every living cell. When an ATP molecule
is hydrolysed, it loses one of its phosphate groups and some energy is released,
which can be used by the cell. In this process, the ATP is converted to ADP
(adenosine diphosphate — Figure 12.2).
ATPase
H2O + +
Figure 12.2 Hydrolysis of ATP and formation of ADP

Cells use energy for many different purposes. These include:
l the synthesis of proteins and other large molecules from smaller ones,
including DNA replication. These are examples of anabolic reactions —
that is, energy-consuming reactions.
l the active transport of ions and molecules across cell membranes against
their concentration gradient (p. 37)
l the transmission of nerve impulses (p. 130)
l movement, for example muscle contraction (such as heartbeat, breathing
movements, walking) or movement of cilia
l the production of heat to maintain body temperature at a steady level (in
mammals and birds)
Each cell makes its own ATP. The hydrolysis of one ATP molecule releases a
small ‘packet’ of energy that is often just the right size to fuel a particular step
in a process. A glucose molecule, on the other hand, would contain far too
much energy, so a lot would be wasted if cells used glucose molecules as their
immediate source of energy.
All cells make ATP by respiration. This is described in the next few pages.

Respiration

Respiration is a series of enzyme-
controlled reactions that takes place in
All cells obtain useable energy through respiration. Respiration is the oxidation cells, in which energy within a nutrient
of energy-containing organic molecules, such as glucose. These are known as molecule (e.g. glucose) is used to make
respiratory substrates. The energy released from this process is used to ATP.
combine ADP with inorganic phosphate (Pi) to make ATP.
Respiration can take place in aerobic or anaerobic conditions. In both cases,
glucose or another respiratory substrate is oxidised.
In aerobic respiration, oxygen is involved, and the substrate is oxidised
completely, releasing much of the energy that it contains. Typical mistake
In anaerobic conditions, respiration takes place without oxygen, and the Do not say that respiration ‘produces’
or ‘makes’ energy. The energy is already
substrate is only partially oxidised. Only a small proportion of the energy it there, in the glucose molecule.
contains is released.
Coenzymes Revised
Respiration involves coenzymes called NAD, FAD and coenzyme A. A

coenzyme is a molecule required for an enzyme to be able to catalyse a reaction.
NAD and FAD are reduced during respiration. The term ‘reduce’ means to add
hydrogen, so reduced NAD has had hydrogen added to it. Without the presence
of NAD or FAD to accept the hydrogen, the dehydrogenase enzymes involved in
respiration would not be able to remove hydrogen from their substrates.
Aerobic respiration Revised
Glucose, C6H12O6 (or another respiratory substrate) is split to release carbon

dioxide as a waste product. The hydrogen from the glucose is combined with
atmospheric oxygen. This releases a large amount of energy, which is used to
drive the synthesis of ATP.
Now test yourself
Glycolysis 1 Suggest why glucose must be
Glycolysis is the first stage of respiration. It takes place in the cytoplasm. phosphorylated before the 6C
l A glucose (or other hexose sugar) molecule is phosphorylated, as two ATPs molecule can be split into two 3C
donate phosphate to it. molecules.
l This produces a fructose 1,6-bisphosphate molecule (6C), which splits into Answer on p.203
two triose phosphate molecules. Tested
l Each triose phosphate is oxidised to form a pyruvate molecule. This involves
the removal of hydrogens by dehydrogenase enzymes, which are taken up by
the coenzyme NAD. The removal of hydrogens is an oxidation reaction.
It can also be referred to as dehydrogenation. This produces reduced
NAD. During this step, the phosphate groups from the triose phosphates are
added to ADP to produce a small yield of ATP, in substrate-linked reactions.
l Overall, two molecules of ATP are used and four are made during glycolysis
of one glucose molecule, making a net gain of two ATPs per glucose
(Figure 12.3).
The link reaction

If oxygen is available, each pyruvate now moves into a mitochondrion (Figure
12.4), where the link reaction and the Krebs cycle take place. During these
processes, the glucose is completely oxidised.
Energy and respiration 101

Note: the number of Hexose sugar (e.g. glucose)
grey circles shows the
number of carbon 2 × ATP
atoms in one
2 × ADP
molecule of the P P
compound. Fructose 1,6 bisphosphate
P P
Triose phosphate Triose phosphate
NAD ADP + Pi NAD ADP + Pi
reduced NAD ATP reduced NAD ATP
ADP ADP
ATP ATP
Pyruvate Pyruvate
Figure 12.3 Glycolysis
Envelope
Intermembranal Outer membrane Inner membrane

space folded into cristae
Matrix
ATPases
12 Energy an
0.1 µm
Figure 12.4 A mitochondrion
Carbon dioxide is removed from the pyruvate. This is called decarboxylation.
This carbon dioxide diffuses out of the mitochondrion and out of the cell.
Hydrogen is also removed from the pyruvate, and is picked up by NAD,
producing reduced NAD. This converts pyruvate into a 2C compound. This
immediately combines with coenzyme A to produce acetyl coenzyme A,
which is also a 2C compound.
The Krebs cycle

We have seen that acetyl coenzyme A has two carbon atoms. It combines with
a 4C compound called oxaloacetate to produce a 6C compound, citrate. The
citrate is gradually converted to the 4C compound again through a series of
enzyme-controlled steps (Figure 12.5). These steps all take place in the matrix of
the mitochondrion, and each is controlled by specific enzymes.
During this process:
l more carbon dioxide is released (decarboxylation) and diffuses out of the
mitochondrion and out of the cell
l more hydrogens are released (dehydrogenation) and picked up by NAD and
another coenzyme called FAD; this produces reduced NAD and reduced
FAD
l some ATP is produced from ADP, in substrate-linked reactions

Acetyl coenzyme A
Coenzyme A
Oxaloacetate Citrate
CO2
2 × Reduced NAD
NAD
2 × NAD
Reduced NAD
Reduced FAD
CO2
FAD
ATP ADP + Pi
Figure 12.5 The Krebs cycle Now test yourself
Oxidative phosphorylation 2 How many protons and electrons
The hydrogens picked up by NAD and FAD are now split into electrons and are there in one hydrogen atom?
protons. The electrons are passed along the electron transport chain, on the Answer on p.203
inner membrane of the mitochondrion. Tested
As the electrons move along the chain, they release energy. This energy is
used to actively transport protons (hydrogen ions) from the matrix of the
mitochondrion, across the inner membrane and into the space between the
inner and outer membranes. This builds up a high concentration of protons in
this space.
The protons are allowed to move back by facilitated diffusion into the matrix
through special channel proteins that work as ATP synthases. The movement of
the protons through the ATP synthase provides enough energy to cause ADP
and inorganic phosphate to combine to make ATP.
At the end of the chain, the electrons reunite with the protons from which
they were originally split. They combine with oxygen to produce water (Figure
12.6). This is why oxygen is required in aerobic respiration — it acts as the final
acceptor for the hydrogens removed from the respiratory substrate during
glycolysis, the link reaction and the Krebs cycle.
Intermembranal space ATP synthase
Inner membrane
H+ O2
+
+ H
H+ H
e−
NAD Electron
Electron Electron
carrier
e− carrier carrier
H2O ADP + Pi ATP
H+
Reduced
NAD H+
H+
The electron transport chain The protons move by
provides energy to transport H+ facilitated diffusion back
protons from the matrix to through ATP synthase
the space between the inner providing energy to
and outer membranes. make ATP.
Figure 12.6 Oxidative phosphorylation

The relationship between structure and function of
mitochondria
Look back at Figure 12.4 on p. 102.
l A mitochondrion is surrounded by an envelope (two membranes) that
separate it from the cytoplasm, so that the reactions that take place inside it
are not affected by reactions elsewhere in the cell.
l The inner membrane is folded to form cristae, providing a large area in
which the carriers of the electron transport chain, and ATP synthase, can be
embedded.
l The space between the two membranes (intermembrane space) is available
to build up a high concentration of protons.
l The matrix of the mitochondrion contains all the enzymes needed for the
Krebs cycle.
l The matrix of the mitochondrion also contains DNA and ribosomes, which
are used to synthesise some of the proteins needed for the reactions of
respiration.
Respiration in anaerobic conditions Revised
If oxygen is not available, oxidative phosphorylation cannot take place, because

there is nothing to accept the electrons and protons at the end of the electron
transport chain. This means that reduced NAD is not reoxidised, so the
mitochondrion quickly runs out of NAD or FAD that can accept hydrogens
from the Krebs cycle reactions. The Krebs cycle and the link reaction therefore
come to a halt.
Glycolysis, however, can still continue, so long as the pyruvate produced at the
end of it can be removed and the reduced NAD can be converted back to NAD.
The lactate pathway

In mammals, the pyruvate is removed by converting it to lactate (Figure 12.7).
Hexose sugar (glucose)
2 × triose phosphate
Reduced
NAD
NAD
2 × pyruvate 2 × lactate
Figure 12.7 The lactate pathway

The lactate that is produced (usually in muscles) diffuses into the blood and is
carried in solution in the blood plasma to the liver. Here, liver cells convert it
back to pyruvate. This requires oxygen, so extra oxygen is required after exercise
has finished. The extra oxygen is known as the oxygen debt. Later, when the
exercise has finished and oxygen is available again, some of the pyruvate in the
liver cells is oxidised through the link reaction, the Krebs cycle and the electron
transport chain. Some of the pyruvate is reconverted to glucose in the liver
cells. The glucose may be released into the blood or converted to glycogen and
stored.
The ethanol pathway

In yeast and in plants, the pyruvate is removed by converting it to ethanol
(Figure 12.8, overleaf).

Hexose sugar (glucose)
2 × triose phosphate
Reduced
NAD
NAD
2 × pyruvate 2 × ethanal 2 × ethanol
Ethanol
dehydrogenase
Figure 12.8 The ethanol pathway
Adaptations of rice for wet conditions

Rice is often grown in fields that are flooded with water during the growing
season. The roots therefore have little oxygen available to them. Rice has
adaptations that allow it to grow successfully even when respiration in root cells
takes place in these anaerobic conditions (Table 12.1).
Table 12.1 Adaptations of rice for growth with roots submerged in water
How it helps the plant to survive

Feature when roots are submerged
Cells are tolerant of high When roots are submerged in water,
concentrations of ethanol less oxygen is available than when the
soil contains air spaces; cells therefore
respire anaerobically, producing ethanol
Stems have tissue called aerenchyma, Aerenchyma allows oxygen from the air
containing large air spaces to diffuse down to the roots
Some types of rice are able to grow The leaves remain exposed to the air,
elongated stems to keep their leaves which facilitates gas exchange for
above the water as its level rises photosynthesis and respiration
ATP yield in aerobic and anaerobic respiration Revised
Only small amounts of ATP are produced when one glucose molecule
undergoes anaerobic respiration. This is because only glycolysis is completed.
The Krebs cycle and oxidative phosphorylation, which produce most ATP, do
not take place.
The precise number of molecules of ATP produced in aerobic respiration of one
glucose molecule varies between different organisms and different cells, but is
usually between 30 and 32 molecules. Figure 12.9 summarises the four stages in
aerobic respiration, and also shows ATP yields of each stage.

Glucose
Anaerobic
respiration 2 ATP
produced directly
Glycolysis
2 reduced
NAD
Pyruvate
Link 2 reduced
reaction NAD
Acetyl CoA
Aerobic
5 ATP from oxidative
respiration
phosphorylation
5 ATP from oxidative

phosphorylation
Krebs 6 reduced 15 ATP from oxidative
cycle
NAD phosphorylation
2 reduced 3 ATP from oxidative

FAD phosphorylation
2 ATP
produced directly
32 ATP
Figure 12.9 A summary of the four stages of respiration
Respiratory substrates Revised
Glucose is not the only respiratory substrate. All carbohydrates, lipids and
proteins can also be used as respiratory substrates (Table 12.2). Lipid provides
more than twice as much energy per gram as carbohydrate or protein. This
is because a lipid molecule contains relatively more hydrogen atoms (in
comparison with carbon or oxygen atoms) than carbohydrate or protein
molecules do. You have seen that it is hydrogen atoms that provide the protons
that are used to generate ATP via the electron transport chain.
Table 12.2 Energy values of different respiratory substrates
Respiratory substrate Energy released/kJ g−1

Carbohydrate 16
Lipid 39
Protein 17
Many cells in the human body are able to use a range of different respiratory
substrates. However, brain cells can only use glucose. Heart muscle preferentially
uses fatty acids.
Respiratory quotients
It is possible to get a good idea of which respiratory substrate the cells in an
organism are using by measuring the volume of oxygen it is taking in and the
volume of carbon dioxide it is giving out.
volume of CO2 given out
The respiratory quotient, RQ is
volume of O2 taken in

The values in Table 12.3 are for aerobic respiration. If a cell or an organism is Typical mistake

respiring in anaerobic conditions, then no oxygen is being used. The RQ is Do not confuse RQ with Rf values (see
therefore infinity (∞). p. 112).
Table 12.3 RQs for different substrates undergoing aerobic respiration
Respiratory substrate RQ
Carbohydrate 1.0
Lipid 0.7
Protein 0.9

How to… Use respirometers
There are different types of respirometer. One type is shown in The carbon dioxide given out is absorbed by the soda lime. The
Figure 12.10. distance moved by the fluid is therefore affected only by the
oxygen taken up and not by the carbon dioxide given out.
Spring clip Spring clip
You would not expect the manometer fluid in the tube
with no organisms to move, but it may do so because of
temperature changes. This allows you to control for this variable,
by subtracting the distance moved by the fluid in the control
manometer from the distance moved in the experimental
Soda Soda manometer (connected to the living organisms), to give you an
lime lime adjusted distance moved.
50
50
50
50
Cotton Cotton Calculate the mean (adjusted) distance moved by the

40
40
40
40
wool wool manometer fluid per minute. If you know the diameter of the
30
30
30
30
Live Beads capillary tube, you can convert the distance moved to a volume:
20
20
20
20
maggots volume of liquid in a tube = length × πr2

10
10
10
10
0
This gives you a value for the volume of oxygen absorbed by the
Manometer Manometer organisms per minute.
containing containing Using a respirometer to investigate the effect of tempera-
coloured fluid coloured fluid
ture on the rate of respiration
Figure 12.10 A respirometer The respirometer can be placed in water baths at different
Using a respirometer to measure the rate of uptake of temperatures. You can use the same respirometer for the
oxygen whole experiment. Or you could have different ones for each
The organisms to be investigated are placed in one tube, and temperature. (In each case, there are difficulties with controlling
non-living material of the same mass in the other tube. Soda some variables — you might like to think about what these are.)
lime is placed in each tube, to absorb all carbon dioxide. Cotton At each temperature, you need a control respirometer with no
wool prevents contact of the soda lime with the organisms. organisms in it.
Coloured fluid is poured into the reservoir of each manometer If you are simply comparing the rates of respiration at different
and allowed to flow into the capillary tube. It is essential that temperatures, then you do not need to convert the distance
there are no air bubbles. You must end up with exactly the moved by the manometer fluid to a volume. You could just plot
same quantity of fluid in the two manometers. distance moved on the y-axis of your graph and time on the
x-axis.
Two rubber bungs are now fitted with tubes as shown in the
diagram. Close the spring clips. Attach the manometers to the The rate of respiration is represented by the gradient of the
bent glass tubing, ensuring an airtight connection. Next, place graph (Figure 12.11).
the bungs into the tops of the tubes. At 50 °C manometer fluid travelled 47 mm in 50 s
47
Open the spring clips. (This allows the pressure throughout the Rate of respiration = = 0.94 mm s−1
50
apparatus to equilibrate with atmospheric pressure.) Note the
At 30 °C manometer fluid travelled 40 mm in 60 s
level of the manometer fluid in each tube. Close the clips. Each
40
minute, record the level of the fluid in each tube. Rate of respiration = = 0.67 mm s−1
60
As the organisms respire, they take oxygen from the air around At 20 °C manometer fluid travelled 21 mm in 70 s
them and give out carbon dioxide. The removal of oxygen 24
from the air inside the tube reduces the volume and pressure, Rate of respiration = = 0.34 mm s−1
70
causing the manometer fluid to move towards the organisms. ➞

Distance 50
moved by 50ºC
30ºC
manometer
fluid/mm 40
30
20ºC
20
10
0
0 20 40 60 80
Time/s
Figure 12.11 Comparing rates of respiration at different temperatures
Using a respirometer to measure RQ
For this, we need to know both how much oxygen is taken in,
and how much carbon dioxide is given out. distance moved by fluid in experimental tube = x mm
Set up two respirometers as shown in Figure 12.10. However, distance moved by fluid in control tube = y mm
the second respirometer should also contain the same mass x mm represents the oxygen taken up
of live maggots (or whatever organism you are investigating)
but should not contain soda lime. You could put some inert x − y represents the carbon dioxide given out
material into the tube (for example, the beads) instead of soda x−y
therefore RQ =
lime. The mass and volume of the inert material should be the x
same as the mass and volume of the soda lime. For example, if the respiratory substrate is carbohydrate, then
the amount of carbon dioxide given out will equal the amount
This second tube is therefore just like the first one except that of oxygen taken in. The fluid in the control tube will not move,
it does not contain soda lime. The carbon dioxide given out by so y = 0.
the respiring organisms is therefore not absorbed.
x−0
The difference between the distance moved by the manometer RQ is then =1
x
fluid in the experimental tube and the distance moved in the
control tube is therefore due to the carbon dioxide given out.
3 Predict and explain what would happen to the levels of fluid in the manometers if no soda lime was used.
Answer on p.203

How to… Use a redox indicator to investigate rate of respiration in yeast
l We have seen that respiration involves stages in which l You can place a suspension of yeast in glucose solution into
hydrogen is removed from substrate, a process called two boiling tubes. To one of the tubes, add your indicator.
dehydrogenation. We can investigate the rate of Note the time taken for the indicator to change colour. As
dehydrogenase reactions using an indicator that can accept the yeast suspension is not colourless, it is helpful to compare
the hydrogens removed in respiration, and that changes the colour of the tube with the indicator with the tube to
colour when it is reduced (has hydrogens added) or oxidised which you did not add indicator.
(has hydrogens removed). Suitable indicators include l You can use this technique to investigate the effect of
methylene blue and DCPIP. Both of these indicators are temperature or substrate concentration on the rate of
blue when oxidised, and become colourless when they are respiration. To vary temperature, use water baths. To vary
reduced. substrate concentration, change the amount of glucose in the
yeast suspension. Measure the time taken for the indicator to
become colourless.

13 Photosynthesis
An overview of
photosynthesis
Photosynthesis is a series of reactions in which energy transferred as light is
transformed to chemical energy. Energy from light is trapped by chlorophyll, and
this energy is then used to:
l split apart the strong bonds in water molecules to release hydrogen
l produce ATP
l reduce a substance called NADP
NADP stands for nicotinamide adenine dinucleotide phosphate, which — like

NAD — is a coenzyme.
The ATP and reduced NADP are then used to add hydrogen to carbon dioxide,
to produce carbohydrate molecules such as glucose (Figure 13.1). These complex
organic molecules contain some of the energy that was originally in the light. Typical mistake
The oxygen from the split water molecules is a waste product, and is released Students often say that carbon
into the air. dioxide is changed to oxygen in
photosynthesis. Look at Figure 13.1 and
There are many different steps in photosynthesis, which can be divided into two
see for yourself why this is not correct.
main stages — the light dependent stage and the light independent stage.
Light Chloroplast Plant cell
H2O Light dependent stage O2
CO2 Light independent stage C6H12O6
Figure 13.1 An overview of photosynthesis
Chloroplasts Revised
Photosynthesis takes place inside chloroplasts. Like mitochondria, these are

organelles surrounded by two membranes, called an envelope (Figure 13.2).
They are found in mesophyll cells in leaves. Palisade mesophyll cells contain most
chloroplasts but they are also found in spongy mesophyll cells. Guard cells also
contain chloroplasts. You can see a diagram of the structure of a leaf on p. 117.
The membranes inside a chloroplast are called lamellae, and it is here that
the light dependent stages take place. The membranes contain chlorophyll
molecules, arranged in groups called photosystems. There are two kinds of
photosystem, PSI and PSII, each of which contains slightly different kinds of
chlorophyll.
Photosynthesis 109

13 Photosynthesis
Lamella Thylakoid Outer membrane
Envelope
Inner membrane
Stroma
Starch grain
Lipid
droplet
Ribosomes
Granum
Figure 13.2 Structure of a chloroplast
There are enclosed spaces between pairs of membranes, forming fluid-filled
sacs called thylakoids. These are involved in photophosphorylation — the
formation of ATP using energy from light. Thylakoids are often arranged in
stacks called grana (singular: granum).
The ‘background material’ of the chloroplast is called the stroma, and this is
where the light independent stage takes place.
Revision activity
Chloroplasts often contain starch grains and lipid droplets. These are stores of l Compare the structures of a
energy-containing substances that have been made in the chloroplast but are mitochondrion and a chloroplast.
not immediately needed by the cell or by other parts of the plant.
Chloroplast pigments Revised
A pigment is a substance that absorbs light of some wavelengths but not

others. The wavelengths that is does not absorb are reflected from it.
Chlorophyll is the main pigment contained in chloroplasts. It looks green
because it reflects green light. Other wavelengths (colours) of light are absorbed.
Figure 13.3 shows the wavelengths of light absorbed by the various pigments
found in chloroplasts. These graphs are called absorption spectra.
Amount of Key
light absorbed Chlorophyll a
Chlorophyll b
Carotene
400 500 600 700

Wavelength of light/nm
Figure 13.3 Absorption spectra for chloroplast pigments
If we shine light of various wavelengths on chloroplasts, we can measure the
rate at which they give off oxygen. This graph is called an action spectrum
(see Figure 13.4).

13 Photosynthesis
Rate of
photosynthesis
Now test yourself

1 Explain the similarities between
the absorption spectrum and the
action spectrum.
400 500 600 700
Wavelength of light/nm
Answer on p.203
Tested
Figure 13.4 Action spectrum for chloroplast pigments
Chlorophyll a is the most abundant pigment in most plants. Its absorption
peaks are 430 nm (blue) and 662 nm (red). It emits an electron when it
absorbs light.
Chlorophyll b is similar to chlorophyll a, but its absorption peaks are 453 nm
and 642 nm. It has a similar role to chlorophyll a, but is not as abundant.
Carotenoids are accessory pigments. They are orange pigments that protect
chlorophyll from damage by the formation of single oxygen atoms (free radicals).
They can also absorb wavelengths of light that chlorophyll cannot absorb, and
pass on some of the energy from the light to chlorophyll.
Xanthophylls are also accessory pigments, capturing energy from wavelengths
of light that are not absorbed by chlorophyll.

How to… Separate chlorophyll pigments by chromatography
Chromatography is a method of separation that relies on the instead of paper. Only an outline of the procedure is given here,
different solubilities of different solutes in a solvent. A mixture so you cannot use these instructions to actually carry out the
of chlorophyll pigments is dissolved in a solvent, and then a experiment. You can find more details about this technique at
small spot is placed onto chromatography paper. The solvent www.saps.org.uk/secondary/teaching-resources/181-student-
gradually moves up the paper, carrying the solutes with it. The sheet-10-thin-layer-chromatography-for-photosynthetic-
more soluble the solvent, the further up the paper it is carried. pigments. Your teacher should give you an opportunity to
There are various methods. The one described in Figure 13.5 do a chromatography experiment, perhaps using a different
uses thin layer chromatography on specially prepared strips technique from the one described here.
Cut a TLC plate into narrow strips, Put 2 or 3 grass leaves on a slide. Add 6 drops of propanone
about 1.25 cm wide, so they fit into Using another slide scrape the leaves to the extract and mix.
a test tube. Do not put your fingers to extract cell contents.
on the powdery surface.
Figure 13.5
➞
Photosynthesis 111

13 Photosynthesis
Hodder CIE Bio Revision 09

watch glass2.ai Hodder CIE Bio Revision 09
test tube TLC1.indd watch glass2.ai
test tube TLC1.indd
Transfer the mixture to a watch glass. Transfer tiny amounts of the Touch very briefly with the fine tip of the
Allow this to dry out almost completely concentrated extract onto a spot brush and let that spot dry before adding
— a warm air flow will speed this up. 1 cm from one end of the TLC strip. more. Keep the spot to 1 mm diameter if
you can. The final spot, called the origin,
should be small but dark green.
Put the TLC strip in a test tube. Add the running solvent to the After about 4 minutes remove the TLC
Mark the tube below the pigment depth of the mark, then return strip. Immediately mark the solvent front
spot and remove the TLC strip. the TLC strip to the tube and with a needle. You can also mark the
seal it. centres of the pigment spots and the origin.
l Measure the distance from the start line to the solvent front. l You can use the Rf values to help you to identify the
Measure the distances of each pigment spot from the start pigments. Rf values differ depending on the solvent you have
line. For each spot, calculate the Rf value: used, but typical values might be:
distance from start line to pigment spot chlorophyll a 0.60
Rf =
distance from start line to solvent front chlorophyll b 0.50
carotene 0.95
xanthophyll 0.35
You may also see a small grey spot with an Rf value of about
0.8. This is phaeophytin, which is not really a chlorophyll
pigment, but is a breakdown product generated during the
extraction process.
The light dependent stage Revised
Chlorophyll molecules in PSI and PSII absorb light energy. The energy excites
The light dependent stage of
electrons, raising their energy level so that they leave the chlorophyll. The
photosynthesis is the series of events
chlorophyll is said to be photoactivated.
that only occurs when light is present; it
PSII contains an enzyme that splits water when activated by light. This reaction is involves the use of energy from light to
called photolysis (‘splitting by light’). The water molecules are split into oxygen split water molecules and produce ATP
and hydrogen atoms. Each hydrogen atom then loses its electron, to become and reduced NADP.
a positively charged hydrogen ion (proton), H+. The electrons are picked up by
the chlorophyll in PSII, to replace the electrons they lost. The oxygen atoms join
together to form oxygen molecules, which diffuse out of the chloroplast and
into the air around the leaf.
light
2H2O 4H+ + 4e− + O2
The electrons emitted from PSII are picked up by electron carriers (electron
transport chain) in the membranes of the thylakoids. They are passed along a

chain of these carriers, losing energy as they go. The energy they lose is used
13 Photosynthesis
to make ADP combine with a phosphate group, producing ATP. This is called
photophosphorylation. At the end of the electron carrier chain, the electron is
picked up by PSI, to replace the electron the chlorophyll in PSI had lost.
The electrons from PSI are passed along a different chain of carriers to
NADP. The NADP also picks up the hydrogen ions from the split water
molecules. The NADP becomes reduced NADP.
We can show all of this in a diagram called the Z-scheme (Figure 13.6). The
higher up the diagram, the higher the energy level. If you follow one electron
from a water molecule, you can see how it:
l is taken up by PSII
l has its energy raised as the chlorophyll in PSII absorbs light energy
l loses some of this energy as it passes along the electron carrier chain
l is taken up by PSI
l has its energy raised again as the chlorophyll in PSI absorbs light energy
l becomes part of a reduced NADP molecule
Photosystem Chain of
High energy I electron carriers
electron e−
Oxidised
+ H+
Photosystem NADP
II ADP + Pi
e− e− Reduced
ATP
Light NADP
Energy
level
e−
Light
H2O Chain of Expert tip

electron carriers Remember that the light dependent
e−
O2 +
H stage gets the energy to drive the
reactions from light. It is therefore not
Figure 13.6 Summary of the light dependent stage of photosynthesis — the affected by temperature.
Z-scheme
At the end of this process, two new substances have been made. These are Now test yourself
ATP and reduced NADP. Both of them will now be used in the next stage of
photosynthesis, the light independent stage. 2 The Z-scheme shows that electrons
lose energy as they pass along the
Non-cyclic and cyclic photophosphorylation chain of electron carriers. Where
The sequence of events just described and shown in Figure 13.6 is known as does this energy go?
non-cyclic photophosphorylation. Answer on p.203
There is an alternative pathway for the electron that is emitted from PSI. Tested
It can simply be passed along the electron transport chain, then back to
PSI again. ATP is produced as it moves along the electron transport chain
(photophosphorylation). However, no reduced NADP is produced. This is
called cyclic photophosphorylation.
The light independent stage Revised
The light independent stage is made up a cycle of reactions known as the Typical mistake
Calvin cycle (Figure 13.7). It takes place in the stroma of the chloroplast, Do not call the light independent stage
where the enzyme ribulose bisphosphate carboxylase, usually known as ‘the dark stage’. Although this stage
rubisco, is found. does not need light, it can take place in
the light.
Photosynthesis 113

Carbon dioxide diffuses into the stroma from the air spaces within the leaf. It
13 Photosynthesis
The light independent stage of
enters the active site of rubisco, which combines it with a 5C compound called photosynthesis is a series of reactions
ribulose bisphosphate, RuBP. The products of this reaction are two 3C that can take place even when light is
molecules, glycerate 3-phosphate, GP. The combination of carbon dioxide with not present; it uses ATP and NADP from
RuBP is called carbon fixation. the light dependent stage to synthesise
Energy from ATP and hydrogen from reduced NADP are then used to reduce carbohydrates from carbon dioxide.
the GP to triose phosphate, TP. Triose phosphate is the first carbohydrate
produced in photosynthesis.
Most of the triose phosphate is used to regenerate ribulose bisphosphate,
Now test yourself
so that more carbon dioxide can be fixed. The rest is used to make glucose 3 What are the substrate and
or whatever other organic substances the plant cell requires. These include product of rubisco?
polysaccharides such as starch for energy storage and cellulose for making cell 4 Where do the ATP and NADP used
walls, sucrose for transport, amino acids for making proteins, lipids for energy in the Calvin cycle come from?
storage and nucleotides for making DNA and RNA. Answers on p.203
CO2 Tested
Rubisco
Ribulose bisphosphate (RuBP) Intermediate

compound
ADP
ATP
2 × Triose phosphate (TP) 2 × Glycerate 3-phosphate (GP)

Triose phosphate
can be used to make
glucose, other ADP + Pi Reduced NADP
carbohydrates,
lipids or amino acids.
ATP NADP
Figure 13.7 The Calvin cycle
Limiting factors in
photosynthesis
The rate at which photosynthesis takes place is directly affected by several
environmental factors.
l Light intensity. This affects the rate of the light dependent stage, because
this is driven by energy transferred in light rays.
l Temperature. This affects the rate of the light independent stage. At higher
temperatures, molecules have more kinetic energy so collide more often
and are more likely to react when they do collide. (The rate of the light
dependent stage is not affected by temperature, as the energy that drives
this process is light energy, not heat energy.)
l Carbon dioxide concentration in the atmosphere. Carbon dioxide is a
reactant in photosynthesis. Normal air contains only about 0.04% carbon
dioxide.
l Availability of water. Water is a reactant in photosynthesis, but there
is usually far more water available than carbon dioxide, so even if water
supplies are low this is not usually a problem. However, water supply can
affect the rate of photosynthesis indirectly, because a plant that is short of
water will close its stomata, preventing carbon dioxide from diffusing into
the leaf.

If the level of any one of these factors is too low, then the rate of photosynthesis
13 Photosynthesis
A limiting factor is a factor that, when
will be reduced. The factor that has the greatest effect in reducing the rate is in short supply, limits the rate of a
said to be the limiting factor (Figure 13.8). reaction or process.
Over this range of the graphs, Over this range of the graph, light
light intensity is the limiting intensity is not a limiting factor. If light
factor. If light intensity intensity increases, there is no effect
increases, then the rate of on the rate of photosynthesis. Some
photosynthesis increases. other factor is limiting the rate.
Rate of
photosynthesis High CO2
concentration
Here, carbon dioxide is the

limiting factor. We can tell
this is so because when the
concentration of carbon
dioxide is increased, the
Lower CO2 rate of photosynthesis
concentration increases (see top curve).
Light intensity
Figure 13.8 Limiting factors for photosynthesis
Commercial plant growers can manipulate these factors to maximise the rate of
photosynthesis and therefore increase the yields of their crops. They can:
l grow crops in glasshouses, where they can control light intensity,
temperature and carbon dioxide concentration
l cover crops growing in open fields with transparent plastic (e.g. as
polytunnels) to increase temperature
l irrigate crops growing in open fields
How to… Investigate the effect of environmental factors on the rate of photosynthesis of whole plants
One way to measure the rate of photosynthesis is to measure the rate at which oxygen is given off by an aquatic plant such as
Elodea or Cabomba. There are various ways in which oxygen can be collected and measured. One method is shown in Figure 13.9.
Oxygen – the length of this If bubbles need to be cleared

bubble, collected over a from the tube, this reservoir
measured time, represents provides water to do this.
the rate of photosynthesis.
Capillary tube
OFF
0 10 20 30 40 50 60 70 80 90 100
Oxygen collects in the flared The three-way tap is turned so that a

end of the capillary tube over a connection is made between the syringe
measured length of time. and the capillary tube (OFF up). The syringe
is very carefully used to pull the oxygen,
A healthy, photosynthesising collected above the plant, into the capillary
water plant has its stem tube. The collection time is noted and the
cleanly cut under water so length of bubble is measured.
that bubbles of oxygen can be
released during photosynthesis.
Figure 13.9 Apparatus for measuring the rate of photosynthesis

➞
Photosynthesis 115

13 Photosynthesis
Alternatively, you can make calcium alginate balls containing You could investigate the following independent variables:
green algae and place them in hydrogencarbonate indicator l Light intensity. You can vary this by using a lamp to shine
solution. As the algae photosynthesise, they take in carbon light onto the plant or algae. The closer the lamp, the higher
dioxide, which causes the pH around them to increase. The the light intensity.
indicator changes from orange, through red to magenta. l Wavelength of light. You can vary this by placing coloured
You can find details of this technique at www.saps.org. filters between the light source and the plant. Each filter will
uk/secondary/teaching-resources/235-student-sheet-23- allow only light of certain wavelengths to pass through.
l Carbon dioxide concentration. You can vary this by adding
photosynthesis-using-algae-wrapped-in-jelly-balls.
sodium hydrogencarbonate to the water around the aquatic
Whichever technique is used, you should change one factor
plant. This contains hydrogencarbonate ions, which are used
(your independent variable) while keeping all others constant
as a source of carbon dioxide by aquatic plants.
(the control variables). The dependent variable will be the rate
l Temperature. The part of the apparatus containing the plant
at which oxygen is given off (measured by the volume of oxygen
or algae can be placed in a water bath at a range of controlled
collected per minute in the capillary tube) or the rate at which
temperatures.
carbon dioxide is used (measured by the rate of change of
colour of the hydrogencarbonate indicator solution).
Expert tip
It is not easy to vary light intensity without also varying temperature, because
the light from a lamp also heats the water. You can try putting a piece of
transparent plastic between the light and the water.
How to… Investigate the rate of photosynthesis using a redox indicator
Photosynthesis, like respiration, involves the acceptance colour is lost is determined by the rate of the light
of hydrogen by a coenzyme. This occurs during the light- dependent stage.
dependent stage, when hydrogen is accepted by NADP. We You can find a detailed protocol for carrying out this
can investigate the rate at which this occurs by adding a redox investigation at www.nuffieldfoundation.org/practical-biology/
indicator, such as DCPIP, to a suspension of chloroplasts. The investigating-light-dependent-reaction-photosynthesis.
indicator takes up the hydrogen ions that are produced as the
You can use this technique to investigate the effect of light
light dependent stage occurs in the chloroplasts, and loses its
intensity or light wavelength on the rate of photosynthesis. See
colour. This is called the Hill reaction. The rate at which the
above for methods of altering these two independent variables.
A redox indicator is a substance that

changes colour when it is oxidised or
reduced.
Leaf structure in C3
and C4 plants
Plants in which photosynthesis takes place as described are called C3 plants.
This is because the first compound that is produced in the Calvin cycle (GP)
contains 3 carbon atoms. Figure 13.10 shows how various features of a leaf of a
C3 plant are adaptations that enable photosynthesis to take place.
Some plants, such as the crops maize and sorghum, are C4 plants. This means
that, instead of first making a 3C compound during the Calvin cycle, they
produce a 4C compound. This is an adaptation to growing in environments
where the temperature and light intensity are high.
At high temperatures and high light intensities, the enzyme rubisco tends to
catalyse the combination of RuBP with oxygen rather than with carbon dioxide.
This is wasteful, and reduces the rate of photosynthesis. It is sometimes called
‘photorespiration’, because it uses oxygen. The leaves of C4 plants have structural
adaptations that prevent photorespiration from taking place.

13 Photosynthesis
The overall shape of most leaves is thin and broad. The The epidermal cells have no chloroplasts, so light passes
thinness allows sunlight to pass through to all the palisade through and reaches the palisade cells. They secrete a
and spongy cells. The broad shape maximises the surface waxy cuticle, which reduces the evaporation of water
area for absorption of sunlight and carbon dioxide. from the upper surface of the leaf.
Most photosynthesis takes Vascular bundles contain xylem vessels

place in the palisade layer, as and phloem sieve tubes. Xylem helps to
this is where the cells contain support the leaf, holding it out flat so it
most chloroplasts. The position can absorb sunlight. Xylem vessels bring
of this layer close to the surface water to the leaf, which is necessary to
of the leaf enables sunlight to keep cells turgid as well as being a
reach it easily. The tall, narrow reactant in photosynthesis. Phloem sieve
cells mean light has to pass tubes take away assimilates such as
through only three cell walls to sucrose, made by photosynthesis.
reach the chloroplasts. The
cells are tightly packed to The lower epidermal cells have no
capture the maximum sunlight. chloroplasts. They may secrete a
waxy cuticle to cut down water
loss, but this is usually thinner
Some photosynthesis takes place than on the upper surface.
in the spongy layer, as there are
some chloroplasts in these cells.
The large air spaces between them Two guard cells surround each stoma. The stomata allow rapid diffusion of
allow easy and rapid diffusion of carbon dioxide into the leaf for photosynthesis, and they connect with the air
carbon dioxide from the stomata spaces inside the spongy layer. When the guard cells are turgid, they bend
to the chloroplasts in the palisade outwards and leave a space between them — the stomatal pore. When they
layer and the spongy layer. are flaccid, they are less bent and the pore between them is closed.
Figure 13.10 Leaf structure in a C3 plant

In a C4 plant, rubisco and RuBP are kept away from the air spaces inside the
leaf, so they do not come into contact with oxygen. The rubisco and RuBP
are inside the chloroplasts of the bundle sheath cells (Figure 13.11). They are
separated from the air spaces by a ring of mesophyll cells. These also contain
chloroplasts, where the light dependent reactions of photosynthesis take place.
Upper epidermis
Mesophyll
Vascular
bundle Bundle sheath cell
Ring of
mesophyll cells
Lower epidermis
Figure 13.11 Section through a leaf of a C4 plant

Carbon fixation happens like this:
l In the mesophyll cells, carbon dioxide combines with a substance called PEP
to form a 4-carbon compound.
l The 4-carbon compound moves into the bundle sheath cells.
l The 4-carbon compound breaks down and releases carbon dioxide.
l The rubisco in the bundle sheath cells catalyses the reaction of the carbon
dioxide with the RuBP.
l The Calvin cycle then proceeds as normal, inside the bundle sheath cells.
The enzymes involved in photosynthesis in maize and sorghum have higher

optimum temperatures than in plants that are not adapted for growth in hot
climates.
Photosynthesis 117

14 Homeostasis
Homeostasis in mammals
Negative feedback Revised
Homeostasis is the maintenance of a relatively constant internal environment

in the body. Mammals are able to control many properties of their internal
environment, including their internal temperature, glucose concentration and
water potential. This is done using negative feedback. Negative feedback
involves:
l the detection of changes in the internal environment or the external
environment (stimuli) by receptors
l the passing of information about these changes to a central control area
l the dissemination of this information to different parts of the body, by
coordination systems Homeostasis is the maintenance of a
l a response to the information by effectors (muscles and glands) to bring the relatively constant environment for cells.
changed feature of the internal environment back towards its set point Negative feedback is a mechanism
by which a change in a particular factor
In negative feedback, a change in one direction — for example, an increase in brings about an action that reverses the
blood glucose concentration — brings about a response by effectors that change.
reverses the change.
Two coordination systems are involved in homeostasis in mammals:
l The nervous system. This is made up of the brain, spinal cord and nerves,
which all contain specialised cells called neurones. Information is passed from
one part of the body to another in the form of electrical impulses that pass
very rapidly along neurones. You can read more about the nervous system
on pp. 128–131.
l The endocrine system. This is made up a number of endocrine glands,
which secrete hormones into the blood. Information is transferred as the
hormones are distributed around the body in the blood.
Thermoregulation Revised
Mammals and birds are able to maintain a fairly constant core (internal)
temperature. In humans, the set point for core temperature is about 37 °C.
Now test yourself
The receptors that detect changes in core temperature are called
thermoreceptors, and are found in the hypothalamus in the brain. 1 Explain the advantages of
maintaining a constant core
Thermoreceptors in the skin detect changes in the external temperature. temperature.
If these receptors detect a temperature rise: Answer on p.204
l Impulses are sent along motor neurones to arterioles in the skin, causing the Tested
muscle in their walls to relax. This causes the arterioles to widen
(vasodilation), allowing more blood to flow to capillaries at the skin surface.
This allows more heat to be lost from the blood to the environment. Typical mistake
l Impulses are also sent along motor neurones to the sweat glands in the skin, Note that the arterioles simply get
wider; they do not move up and down
causing them to secrete more sweat. The water in the sweat evaporates,
in the skin.
taking heat from the skin in order to do this, and therefore cooling the body.

If the receptors detect a temperature fall:
14 Homeostasis
l Impulses are sent along motor neurones to arterioles in the skin, causing
the muscle in their walls to contract. This causes the arterioles to become
narrower (vasoconstriction), allowing less blood to flow to capillaries at
the skin surface. This allows less heat to be lost from the blood to the
environment.
l Impulses are sent along sensory neurones to skeletal muscles, causing
them to contract and relax very rapidly (shivering), generating heat that is
transferred to the blood.
l In mammals other than humans, erector muscles are stimulated to contract,
raising the hairs on end and trapping a layer of air between the hairs and the
skin. Air is a good insulator, so this reduces heat loss from the body.
You can see that both of these sets of responses will help to bring back the
core temperature to its set point. This control mechanism is a good example of
negative feedback.
Control of blood glucose concentration Revised
Blood glucose concentration should remain at a fairly constant value of about

100 mg glucose per 100 cm3 of blood.
l If blood glucose concentration falls well below this level, the person is said to
be hypoglycaemic. Cells do not have enough glucose to carry out respiration,
and so metabolic reactions may not be able to take place and the cells
cannot function normally. This is especially so for cells such as brain cells,
which can only use glucose and not other respiratory substrates. The person
may become unconscious and various tissues can be damaged.
l If blood glucose concentration rises well above this level, the person is said
to be hyperglycaemic. The high glucose concentration decreases the water
potential of the blood and tissue fluid, so that water moves out of cells
down a water potential gradient. Again, unconsciousness can result.
Several hormones are involved in the control of blood glucose concentration by
negative feedback. They include insulin and glucagon.
Both of these are small proteins. They are secreted by patches of tissue called
islets of Langerhans in the pancreas (Figure 14.1). Insulin is secreted by β cells.
Glucagon is secreted by α cells.
Islet of Langerhans
Capillary
α cells
Typical mistake
β cells
Note that it is the pancreas itself
that senses changes in blood glucose
concentration. Many students think
Pancreas cells secreting
it is the hypothalamus, but this is not
pancreatic enzymes —
involved in blood glucose regulation.
note their basal nuclei
Figure 14.1 The histology of the pancreas
The β cells sense when blood glucose concentration rises too high. They
respond by secreting greater quantities of insulin into the blood. The insulin has Typical mistake
several effects, including: Insulin is not an enzyme, and it does
not convert glucose to glycogen.
l causing muscle and adipose tissue cells (fat cells) to absorb more glucose
Insulin activates enzymes in the liver
from the blood that cause this to take place.
l causing liver cells to convert glucose to glycogen for storage
Homeostasis 119

These effects cause the blood glucose concentration to fall.
14 Homeostasis
The α cells sense when blood glucose concentration falls too low. They respond
by secreting greater quantities of glucagon into the blood. This has several
effects, including:
l causing liver cells to break down glycogen to glucose, and releasing it into Revision activity
the blood
l In pencil, draw a flow diagram to
l causing liver cells to produce glucose from other substances such as amino show how temperature is regulated.
acids or lipids Then, using the same diagram,
erase and rewrite words to show
These effects cause blood glucose concentration to rise. how blood glucose concentration is
regulated.
Cyclic AMP
Glucagon has its effect on liver cells by causing the cell to produce a substance
called cyclic AMP. Cyclic AMP is an example of a second messenger. (The
hormone that causes its activation is the ‘first messenger’.) Cyclic AMP then
triggers a series of reactions in the cell that results in the production of an
enzyme that causes the conversion of glycogen to glucose. This is shown in
Figure 14.2.
Outside the liver cell Glucagon
1 Glucagon binds Enzyme
Receptor
to receptor.
Inside the liver cell 3 G protein activates

enzyme. ATP
2 Receptor
activates 4 Activated enzyme produces
G protein. cyclic AMP from ATP.
G protein Cyclic AMP
5 Cyclic AMP activates enzyme cascade.
Inactive protein kinase enzyme Active protein kinase enzyme
Inactive phosphorylase kinase enzyme Active phosphorylase kinase enzyme
6 The enzyme cascade causes the Inactive glycogen Active glycogen

activation of glycogen phosphorylase phosphorylase enzyme phosphorylase enzyme
enzyme, which breaks down
glycogen to glucose.
Glycogen Glucose
Figure 14.2 How glucagon causes the production of glucose from glycogen
This series of reactions is known as an enzyme cascade. Because each enzyme Now test yourself
molecule that is activated can catalyse thousands more reactions, the number of
activated enzymes increases at each step. 2 The enzyme cascade is said to
‘amplify’ the signal from glucagon.
The hormone adrenaline has a similar effect. Adrenaline is secreted by the Suggest what this means.
adrenal glands in a ‘fight or flight’ situation. It is transported in the blood to
liver cells, where it binds to a different set of receptors on their cell surface Answer on p.204
membranes, activating the same enzyme cascade. Tested
The interaction of the hormone with the liver cell is an example of cell
signalling. This type of cell signalling involves:
l interaction between the hormone and a receptor on the cell surface Cell signalling is the transmission
membrane of information between one cell and
another, by means of chemicals that
l formation of cyclic AMP, which binds to kinase proteins
bind with receptors on the cell surface
l an enzyme cascade involving activation of enzymes by phosphorylation to membrane, or within the cell.
amplify the signal

Dip sticks for measuring glucose concentration
14 Homeostasis
Diabetes is a disease in which blood glucose concentration is not effectively
controlled. People with diabetes need to measure their blood glucose
concentration at regular intervals, so that they can control it by diet or by
injecting insulin. Urine can also be tested for the presence of glucose. Normally,
there should be no glucose in urine; its presence indicates diabetes.
Glucose concentration in a liquid, such as blood or urine, can be measured using
dip sticks containing immobilised enzymes (Figure 14.3).
l A small pad at one end of the dip stick contains immobilised glucose
oxidase and peroxidase. It also contains potassium iodide chromogen.
l When the pad is in contact with glucose, the glucose oxidase converts the
glucose to gluconic acid and hydrogen peroxide.
l The peroxidase then catalyses a reaction of the hydrogen peroxide with the
potassium iodide chromogen. Different colours are produced according to
the quantity of hydrogen peroxide formed, which in turn depends on the
original concentration of glucose in the liquid.
Reagents Reactions in the

Dip stick on the stick presence of glucose
immobilised glucose
glucose gluconic acid in urine
oxidase +
H2O2
immobilised
peroxidase
Reactive end colour change
KI and
of dip stick
chromogen
Figure 14.3 How a glucose dip stick works
Biosensors for measuring glucose concentration Now test yourself

A biosensor for glucose contains immobilised glucose oxidase. When in contact
3 Suggest advantages of using a
with glucose, the enzyme converts the glucose to gluconic acid. During this biosensor rather than a dip stick for
reaction, an enzyme cofactor is reduced. The reactions cause a flow of electrons, measuring glucose concentration.
producing a tiny current that is measured and gives a digital readout.
Answer on p.204
Tested
The roles of the kidneys in

homeostasis
The kidneys help to maintain a constant internal environment by:
l excreting waste products, particularly the nitrogenous waste product urea
l helping to control the quantity of water in the body fluids (osmoregulation)
Excretion Revised
Excretion is the removal of waste products generated by metabolic reactions

inside body cells. Some of these products are toxic, while others are simply in
excess of requirements.
Mammals excrete urea. Excess amino acids cannot be stored in the body. In the Typical mistake
liver, they are converted to urea, CO(NH2)2, and a keto acid. This is known as Remember that deamination takes
deamination (Figure 14.4). The keto acid can be respired to provide energy, or place in the liver, not in the kidneys.
converted to fat for storage.
Homeostasis 121

14 Homeostasis
Deamination R Amino group
O H
Amino acid C C N
H O H
H
Organic acid N Ammonia
H H
CO2
Aerobic Urea
Glucose Lipids
respiration CO(NH2)2
Figure 14.4 Formation of urea

The urea dissolves in the blood plasma and is removed and excreted by the
kidneys. Urea contains nitrogen, and so is known as a nitrogenous excretory
product.
The structure and histology of the kidneys Revised
Each kidney is supplied with oxygenated blood through a renal artery. Blood is
removed in the renal vein. A tube called the ureter takes urine from the kidney
to the bladder (Figure 14.5).
Capsule
Branch of renal artery
Cortex
Branch of renal vein Medulla
Pelvis
Ureter
Figure 14.5 Section through a kidney and its associated blood vessels
Each kidney contains thousands of microscopic tubes called nephrons (Figure
14.6). The beginning of each nephron is a cup-shaped structure called a renal
capsule (Bowman’s capsule). This is in the cortex of the kidney. The tube
leads from the renal capsule down into the kidney medulla, then loops back
into the cortex before finally running back down through the medulla into the
pelvis of the kidney, where it joins the ureter.
Renal capsule
Distal convoluted tubule
Proximal Cortex
convoluted tubule Medulla
Loop of Henlé Collecting duct
Pelvis
Figure 14.6 Structure of a nephron
Each nephron has a network of blood vessels associated with it. Blood arrives
in the afferent arteriole (from the renal artery), and is delivered to a network
of capillaries, called a glomerulus, in the cup of the renal capsule (Figure 14.7).
Blood leaves the glomerulus in the efferent arteriole, which is narrower than the
afferent arteriole. This leads to another network of capillaries that wraps around
the nephron, before delivering the blood to a branch of the renal vein.

14 Homeostasis
Afferent arteriole Efferent arteriole
From renal To renal vein
artery
Glomerulus
Renal capsule
Distal
Proximal convoluted convoluted
tubule tubule
Collecting
duct
Loop of Henlé
Figure 14.7 The blood vessels associated with a nephron

Figures 14.8 and 14.9 show the histology of the kidney. Histology is the structure
of tissues.
Cortex Medulla
Capillary
Collecting duct
Renal capsule
Capillary
Glomerulus
Thick segment of
Microvilli
Loop of Henlé
Proximal Thin segment of
convoluted tubule Loop of Henlé
Distal convoluted
tubule
Figure 14.8 Histology of the kidney
Blood capillary
Proximal
convoluted
tubule
Proximal convoluted
tubule epithelium
Glomerular
Microvilli filtrate
Figure 14.9 Longitudinal section of part of a proximal convoluted tubule
How urine is produced in a nephron Revised
Ultrafiltration Ultrafiltration is the separation of

The blood in a glomerulus is separated from the space inside the renal capsule by: large solute molecules from small ones,
l the capillary wall (endothelium) which is one cell thick and has pores in it by passing blood plasma through the
l the basement membrane of the wall of the renal capsule basement membrane of the renal
l the layer of cells making up the wall of the renal capsule, called podocytes; capsule.
these cells have slits between them
Homeostasis 123

The blood in a glomerulus is at a relatively high pressure, because the efferent
14 Homeostasis
arteriole is narrower than the afferent arteriole. This forces molecules from the
blood through these three structures, into the renal capsule. The pores in the
capillary endothelium and the slits between the podocytes will let all molecules
through, but the basement membrane acts as a filter and will only let small
molecules pass through.
Substances that can pass through include water, glucose, inorganic ions such as
Na+, K+ and Cl−, and urea.
Substances that cannot pass through include red and white blood cells and
plasma proteins (such as albumen and fibrinogen).
The liquid that seeps through into the renal capsule is called glomerular filtrate
(Table 14.1).
Table 14.1 Comparison of the composition of blood and glomerular filtrate
Component Blood Glomerular filtrate

Cells Contains red cells, white No cells
cells and platelets
Water/g dm−3 900 900
Inorganic ions (including 7 7
Na+, K+and Cl−)/g dm−3
Plasma proteins/g dm−3 45 0
Glucose/g dm−3 1 1
Urea/g dm−3 0.3 0.3
Selective reabsorption in the proximal convoluted tubule

Some of the substances that are filtered into the renal capsule need to be
Selective reabsorption is the removal
retained by the body. These include:
of particular substances from the
l much of the water
glomerular filtrate, and their return to
l all of the glucose the blood.
l some of the inorganic ions
These substances are therefore taken back into the blood through the walls
of the proximal convoluted tubule. This is called selective reabsorption
(Figure 14.10).
Transport of Na+ Diffusion Active Diffusion

transport
Movement of water Osmosis
Transport of glucose Co-transport Diffusion

(with Na+ diffusion)
Figure 14.10 Selective reabsorption in the proximal convoluted tubule

The cells in the walls of the tubule have many mitochondria, to provide ATP
for active transport. Their surfaces facing the lumen of the tubule have a large
surface area provided by microvilli.
l Active transport is used to move Na+ out of the outer surface of a cell in
the wall of the proximal convoluted tubule, into the blood.
l This lowers the concentration of Na+ inside the cell, so that Na+ ions diffuse
into the cell from the fluid inside the tubule. The Na+ ions diffuse through
protein transporters in the cell surface membrane of the cell.

l As the Na+ ions diffuse through these transporter proteins, they carry
14 Homeostasis
glucose molecules with them. This is called co-transport. The glucose
molecules move through the cell and diffuse into the blood.
l The movement of Na+ and glucose into the blood decreases the water
potential in the blood. Water therefore moves by osmosis from the fluid
inside the tubule, down a water potential gradient through the cells making Now test yourself
up the wall of the tubule and into the blood.
4 Although urea is not added
As a result, the fluid inside the nephron now has: to the liquid inside the
l no glucose proximal convoluted tubule, its
concentration increases. Why is
l a lower concentration of Na+ than the filtrate originally had this?
l less water than the filtrate originally had
Answer on p.204
About 50% of the urea is also reabsorbed in the proximal convoluted tubule. Tested
The loop of Henlé

Some, but not all, nephrons have long loops of Henlé that dip down into the
medulla and then back up into the cortex. The function of the loop of Henlé is
to build up a high concentration of Na+ and Cl− in the tissues of the medulla.
This allows highly concentrated urine to be produced. Note that the loop of
Henlé itself does not produce highly concentrated urine.
As fluid flows down the descending limb of the loop of Henlé, water moves out
of it by osmosis. By the time the fluid reaches the bottom of the loop, it has
a much lower water potential than at the top of the loop. As it flows up the
ascending limb, Na+ and Cl− move out of the fluid into the surrounding tissues,
first by diffusion and then by active transport.
This creates a low water potential in the tissues of the medulla. The longer the
loop, the lower the water potential that can be produced.
The distal convoluted tubule and collecting duct

The fluid inside the tubule as it leaves the loop of Henlé and moves into the
collecting duct has lost a little more water and more Na+ than it had when it
entered the loop. Because more water has been lost, the concentration of urea
has increased.
Now, in the distal convoluted tubule, Na+ is actively transported out of the fluid.
The fluid then flows through the collecting duct. This passes through the
medulla, where you have seen that a low water potential has been produced by
the loop of Henlé. As the fluid continues to flow through the collecting duct,
Revision activity
water moves down the water potential gradient from the collecting duct and
l Construct a flow diagram showing
into the tissues of the medulla. This further increases the concentration of urea
what happens to glomerular filtrate
in the tubule. The fluid that finally leaves the collecting duct and flows into the as it passes through a nephron.
ureter is urine.
Osmoregulation Revised
Osmoregulation is the control of the water content of body fluids. It is part of

homeostasis, the maintenance of a constant internal environment. It is important
Now test yourself
that cells are surrounded by tissue fluid of a similar water potential to their own 5 Explain, using water potential
contents, to avoid too much water loss or gain, which could disrupt metabolism. terminology, what would happen
to a red blood cell if the water
You have seen that water is lost from the fluid inside a nephron as it flows potential of the blood plasma
through the collecting duct. The permeability of the walls of the distal dropped too low.
convoluted tubule and collecting duct can be varied.
l If the walls are permeable to water, then much water can move out of the
Answer on p.204
Tested
tubule and the urine becomes concentrated. The water is taken back into
the blood and retained in the body.
Homeostasis 125

l If the walls are made impermeable to water, little water can move out of
14 Homeostasis
the tubule and the urine remains dilute. A lot of water is removed from
the body.
ADH
ADH is antidiuretic hormone. It is secreted from the posterior pituitary
gland into the blood.
Osmoreceptor cells in the hypothalamus sense when the water potential of
the blood is too low (that is, it has too little water in it). The osmoreceptor cells
are neurones (nerve cells). They produce ADH, which moves along their axons
and into the posterior pituitary gland from where it is secreted into the blood
(Figure 14.11).
Skull
Cerebrum
Hypothalamus
containing
osmoreceptors
ADH is produced in the

hypothalamus and moved
along the axons of
neurones leading to the
posterior pituitary gland.
Posterior pituitary
The ADH is released into
blood vessels and enters Blood vessels
the circulation.
Figure 14.11 Osmoreceptors and the secretion of ADH

The ADH travels in solution in the blood plasma. When it reaches the walls
of the collecting duct, it makes them permeable to water. Water is therefore
reabsorbed from the fluid in the collecting duct and small volumes of
concentrated urine are produced.
The osmoreceptor cells also sense when the water potential of the blood is
too high (that is, it has too much water in it), and less ADH is secreted. The
collecting duct walls therefore become less permeable to water and less is
reabsorbed into the blood. Large volumes of dilute urine are produced.
Negative feedback
The mechanism for controlling the water content of the body, using ADH, is an Revision activity
example of negative feedback. l Use the diagram that you
constructed to show how blood
When the water potential of the blood rises too high or falls too low, this is glucose concentration and
sensed by receptor cells. They cause an action to be taken by effectors, which temperature are controlled, but
cause the water potential to be moved back towards the correct value. now adapt it to show how the
water potential of the blood is
In this case, the receptors are the osmoreceptor cells in the hypothalamus, and controlled.
the effectors are their endings in the anterior pituitary gland that secrete ADH.
Urine analysis
We have seen on p. 121 that urine can be tested for glucose; its presence
indicates diabetes. Urine can also be tested for protein and ketones. The
presence of protein indicates kidney damage or an infection, which is allowing
proteins from the blood to enter the filtrate. It can also be an indication of high
blood pressure. Ketones are produced by the metabolism of fatty acids, and
ketones in urine indicates that they are in higher than normal concentrations in

the blood, suggesting that body cells do not have sufficient supplies of glucose
14 Homeostasis
and are therefore using fatty acids instead. This may indicate diabetes, but can
also indicate that the person is not eating sufficient carbohydrate.
Homeostasis in plants
Plants are not able to control their internal environment in the way that
mammals do. However, they are able to regulate water loss to some extent,
and also respond to changes in environmental conditions, by opening or closing
their stomata.
A stoma is a pore in the epidermis of a plant that is surrounded by a pair of
guard cells (Figure 14.12). Most stomata are in the lower surfaces of leaves.
Water uptake Water uptake
Cells expand
outward
Figure 14.12 A stoma and guard cells

The cell walls of the guard cells are thicker nearer the stomatal aperture than on
their outer edges. When they take up water, their expansion causes the stoma
to open.
l Proton pumps in the guard cell membranes move protons (hydrogen ions)
out of the cells by active transport, which produces a negative electrical
potential inside the cell.
l This causes potassium ion channels in the cell surface membrane to open.
l Potassium ions diffuse into the cell through the open channels, down an
electrochemical gradient.
l This decreases the water potential of the cell, so water moves into the cell by
osmosis, down a water potential gradient.
l This increases the volume of the cell, causing it to swell and curve away from
its partner, opening the stoma.
l In the presence of very high temperatures, or when the plant is short of
water, a plant hormone (plant growth regulator) called abscisic acid, ABA,
is produced.
l It is thought that ABA binds with receptors on the cell surface membranes
of the guard cells, which inactivates the proton pumps.
l ABA also stimulates the uptake of calcium ions into the cell.
l The calcium ions act as a second messenger, activating channel proteins that
allow negatively charged ions (anions) such as chloride ions to move out of
the cell by facilitated diffusion. The calcium ions also cause potassium ion
channels to open, so that potassium ions can leave the cell.
l The exit of chloride ions and potassium ions increases the water potential of
the cell, so water moves out down a water potential gradient by osmosis.
l The guard cells therefore return to their normal shape and close the stoma.
In many plant species, the opening and closing of stomata follows a natural
diurnal (daily) rhythm. Stomata tend to close at night and open in the daytime,
when light levels are high, so that carbon dioxide can diffuse into the leaf and
photosynthesis can take place. However, this rhythm is altered in response to
environmental conditions such as high temperatures or low availability of water,
which can cause stomata to close in the daytime.
Homeostasis 127

In multicellular organisms, such as plants and animals, it is essential that cells can
communicate with each other. This allows them to coordinate their activities
appropriately. Organisms have specialised cells or molecules, called receptors,
which are sensitive to changes in their internal or external environment. These
trigger events in the organism that bring about coordinated responses to the
environmental changes.
Control and coordination

in animals
Neurones Revised
Neurones (nerve cells — Figure 15.1) are highly specialised cells that are adapted
for the rapid transmission of electrical impulses, called action potentials, from
one part of the body to another.
Motor neurone
Nucleus Cytoplasm containing many Myelin sheath Node of Ranvier
ribosomes and mitochondria
Schwann cell
nucleus
Dendrites
Axon
Sensory neurone Relay neurone
Figure 15.1 The structure of neurones

Information picked up by a receptor is transmitted to the central nervous
system (brain or spinal cord) as action potentials travelling along a sensory
neurone. These neurones have their cell bodies in small swellings, called
ganglia, just outside the spinal cord. The impulse may then be transmitted to
a relay neurone, which lies entirely within the brain or spinal cord. The impulse
is then transmitted to many other neurones, one of which may be a motor
neurone. This has its cell body within the central nervous system, and a long
axon, which carries the impulse all the way to an effector (a muscle or gland).
In some cases, the impulse is sent on to an effector before it reaches the
‘conscious’ areas of the brain. The response is therefore automatic, and does not
involve any decision-making. This type of response is called a reflex, and the
arrangement of neurones is called a reflex arc (Figure 15.2).

To brain From brain
Relay neurone — note that not all
reflex arcs include a relay neurone.
Sensory neurone
From touch
receptors Spinal cord
Grey matter
To muscle
effectors Motor neurone
Figure 15.2 Arrangement of neurones in a reflex arc

The myelin sheath is made up of many layers of cell membranes of Schwann
cells, which wrap themselves round and round the axon. This provides electrical
insulation around the axon, which greatly speeds up the transmission of action
potentials. Not all neurones are myelinated.
Resting potential
Neurones, like all cells, have sodium–potassium pumps in their cell surface
The resting potential is a difference in
membranes. However, in neurones these are especially active. They pump out
electrical potential between the outside
sodium ions and bring in potassium ions by active transport. Three sodium ions
and inside of the cell surface membrane
are moved out of the cell for every two potassium ions that are moved in. of a neurone; usually about −70 mV
There are also other channels in the membrane that allow the passage of inside.
sodium and potassium ions. These are voltage-gated channels. The potential
difference across the membrane determines whether they are open or closed. Expert tip
When a neurone is resting, quite a few potassium ion channels are open, so
Always take care to write about
potassium ions are able to diffuse back out of the cell, down their concentration sodium ions and potassium ions,
gradient. not just ‘sodium’ and ‘potassium’.
Alternatively, you can use their
As a result, the neurone has more positive ions outside it than inside it. This symbols: Na+ and K+.
means there is a potential difference (a voltage) across the axon membrane. It
has a charge of about −70 mV (millivolts) inside compared with outside. This is
called the resting potential.
Generation of an action potential by receptors

Receptors are specialised cells that respond to stimuli by generating action potentials.
Figure 15.3 shows an example of a receptor — a taste bud in the tongue.
Taste pore
Microvilli
Receptor cell
Supporting cell
Basal cell
Sensory neurones to brain
Figure 15.3 A taste bud

The cell surface membranes of the microvilli contain proteins that act as
receptors for chemicals in food. When a particular chemical binds with this
receptor, it causes sodium channels to open in the membrane. This allows
sodium ions to diffuse into the cell down an electrochemical gradient and
Control and coordination 129

depolarise it. This quickly reverses the potential difference across the cell
membrane, making it much less negative inside. The neurone is said to be

depolarised. If this depolarisation is relatively small, then nothing further Depolarisation is the reduction or
happens. However, if the depolarisation is great enough, then an action reversal of the resting potential.
potential is triggered. The sodium ions keep on flooding in until the cell has
actually become positive inside, reaching a potential of about +30 mV. The
Expert tip
sodium ion channels then close.
Note that all action potentials are the
This change in potential difference across the membrane causes a set of voltage- same size. Stronger stimuli do not
gated potassium ion channels to open. Potassium ions can now flood out of generate larger action potentials. They
the axon, down their electrochemical gradient. This makes the charge inside the do, however, generate more frequent
axon less positive. It quickly drops back down to a little below the value of the action potentials.
resting potential.
The voltage-gated potassium ion channels then close and the resting potential is
restored (Figure 15.4).
The time taken for the axon to restore its resting potential after an action
potential is called the refractory period. The axon is unable to generate
another action potential until the refractory period is over. The refractory period
therefore places an upper limit for the frequency of nerve impulses.
Sodium Sodium Potassium Potassium
channels channels channels channels
open close open close
Potential difference/ 40
mV 20
0
−20
Now test yourself
−40 1 Explain what causes the opening
−60 and closing of the K+ channels
shown in Figure 15.4.
−80
Answer on p.204
0 1 2 3 4 Tested
Time/ms
Figure 15.4 An action potential
Transmission of action potentials

An action potential or nerve impulse that is generated in one part of a
neurone travels rapidly along its axon or dendron. This happens because the
depolarisation of one part of the membrane sets up local circuits with the areas
on either side of it. These cause depolarisation of these regions as well. The
nerve impulse therefore sweeps along the axon (Figure 15.5).
Action potential Depolarisation of the membrane

creates electric fields.
+ + + − − + + +
− − − + + − − −
The electric fields cause
depolarisation of adjacent
parts of the membrane.
− − − + + − − −
+ + + − − + + +
Figure 15.5 How a nerve impulse travels along a neurone

In a myelinated neurone, local circuits cannot be set up in the parts of the neurone
where the myelin sheath is present. Instead, the nerve impulse ‘jumps’ from one
node of Ranvier to the next. This is called saltatory conduction. This greatly
increases the speed at which the action potential travels along the axon.

Synapses
Where two neurones meet, they do not actually touch. There is a small gap
between them called a synaptic cleft. The membrane of the neurone just
before the synapse is called the presynaptic membrane, and the one on the
other side is the postsynaptic membrane. The whole structure is called a
synapse (Figure 15.6).
Presynaptic Synaptic cleft Postsynaptic membrane
membrane
Vesicle containing Expert tip

transmitter substance Take care not to use the word ‘synapse’
when you mean ‘synaptic cleft’.
Figure 15.6 A synapse
l When an action potential arrives at the presynaptic membrane, it causes
voltage-gated calcium ion channels to open.
l Calcium ions rapidly diffuse into the cytoplasm of the neurone, down their
concentration gradient.
l The calcium ions affect tiny vesicles inside the neurone, which contain a
neurotransmitter such as acetylcholine. These vesicles move towards the
presynaptic membrane and fuse with it, releasing their contents into the cleft.
l The neurotransmitter diffuses across the cleft and slots into receptor
molecules in the postsynaptic membrane.
l This causes sodium ion channels to open, so sodium ions flood into the
cytoplasm of the neurone, depolarising it.
l This depolarisation sets up an action potential in the postsynaptic neurone.
Functions of synapses in the body

l Although action potentials are able to travel in either direction along a Now test yourself
neurone, synapses ensure that action potentials can only travel one way.
l One neurone may have synapses with many other neurones. This allows 2 Explain why a nerve impulse can
interconnection of nerve pathways from different parts of the body. only travel in one direction across a
synapse.
l Synapses allow for a wide variety of responses by effectors. For example,
a motor neurone may need to receive transmitter substances from many Answer on p.204
different neurones forming synapses with it before an action potential Tested
is generated in it. Or some of these neurones may produce transmitter
substances that actually reduce the chance of an action potential being
produced. The balance between the signals from all these different synapses
determines whether or not an action potential is produced in the motor
neurone, and therefore whether or not a particular effector takes action.
Muscles Revised
Muscles are effectors that are able to cause movement. The muscles that are
attached to the skeleton are called skeletal or striated muscles.
Striated muscle tissue is made up of ‘cells’ called muscle fibres. These are not
normal cells, because each one contains several nuclei.
Each muscle fibre is made up of many parallel myofibrils (Figure 15.7). The
myofibrils contain several different proteins, including actin and myosin. The
muscle fibres also contain many mitochondria, which supply the ATP needed
for muscle contraction. The endoplasmic reticulum of the muscle fibre is very

specialised, and forms T-tubules that run from the cell surface membrane right
into the centre of the fibre. Branching from these T-tubules are cisternae in
which calcium ions accumulate.
Part of a muscle fibre Nucleus Myofibril

Muscle fibres are very Myofibrils are collections of
specialised cells containing actin and myosin filaments.
many nuclei and are up to
several centimetres long. Sarcomere
Myofibril
Cytoplasm — contains
myoglobin (for clarity A band I band
organelles such as
mitochondria and ER Cell surface membrane — infolded
not shown) at intervals forming t-tubules
(shown in lower half only)
Detail of a myofibril at the edge of a muscle fibre
Cell surface
membrane Cytoplasm
containing
t-tubule myoglobin
Cisternum of ER
containing Ca2+
Mitochondrion
Figure 15.7 The structure of striated muscle

Actin molecules form thin filaments, and bundles of myosin molecules forms
thick filaments. These filaments are arranged in a very specific pattern within
myofibrils, to form sarcomeres (Figure 15.8). Each myofibril contains many
sarcomeres arranged in a continuous line.
Actin Tropomyosin Troponin
Sarcomere Myosin filament Actin filament

(thick) (thin)
Myosin
Z disc A band I band M disc H band
Figure 15.8 Sarcomeres in a myofibril Figure 15.9 Detailed organisation of the

proteins in part of a sarcomere
Two other proteins, called troponin and tropomyosin, are associated with the
actin filaments (Figure 15.9). In a resting muscle, troponin covers sites on actin to
which myosin would otherwise be able to bind.
3 Use Figures 15.7–15.9 to explain the difference between a muscle fibre, a myofibril and a filament.
Answer on p.204

Muscle stimulation and contraction

Striated muscle is stimulated to contract when a nerve impulse arrives at
a neuromuscular junction. This is like a synapse, but the postsynaptic
membrane is the membrane of a muscle fibre rather than the membrane of a
neurone. When a nerve impulse arrives, the following stages occur:
l The action potential spreads down the membranes of the T-tubules, which
carry it deep into each myofibril.
l The arrival of the action potential opens calcium ion channels in these
membranes, allowing calcium ions to diffuse out of the cisternae and into
the cytoplasm of the myofibril.
l The calcium ions bind to the troponin molecules.
l This causes the troponin molecules to change shape, which in turn causes
the tropomyosin molecules to move away from the binding sites they are
covering.
l The myosin heads can now bind to the actin filaments, forming actin–
myosin bridges.
l The myosin heads tilt, pushing the actin filaments along. The actin filaments
slide between the myosin filaments, causing the Z discs to be pulled closer
together and the sarcomere to get shorter.
l The myosin heads detach from the actin filament, flip back to their normal
shape and reattach. They then tilt again, pushing the actin filament further
along (Figure 15.10). This can be repeated many times a second.
Relaxed muscle In muscle contraction actin and myosin filaments slide past
each other. This shortens each sarcomere.
Contracted
muscle Expert tip
Note that the ATP is used to detach
the myosin from the actin, not to
make it bind or move.
Sarcomere
Figure 15.10 How sliding filaments cause muscle contraction Now test yourself
The energy from this process comes from the hydrolysis of ATP. ATP binds to 4 Explain why muscle fibres contain
the myosin heads after they have tilted. The ATP is then hydrolysed to ADP and many mitochondria.
Pi. The energy that is released causes the myosin heads to tilt back to their Answer on p.204
original position, so that they can bind again with actin. Tested
The mammalian endocrine system Revised
The endocrine system is made up of a number of endocrine glands. These

are organs containing cells that secrete hormones. The hormones are secreted
directly into the blood, not into a duct as with other types of gland (for example
the salivary glands, which secrete saliva into the salivary ducts).
Hormones secreted by an endocrine gland are transported in solution in the
blood plasma all over the body. Certain cells have receptors for these hormones
in their cell surface membranes. These cells are the target cells for the
hormones. For example, the target cells for ADH (p. 126) are the cells lining the
collecting duct in a nephron. The target cells for insulin and glucagon include
liver cells (p. 119).

Reproductive hormones in the menstrual cycle
In humans, the menstrual cycle is a repeating process of change in the ovaries,
oviducts and uterus, which takes place approximately every 28 days from
puberty to menopause. It is controlled by four hormones:
l two steroid hormones secreted by the ovaries — oestrogen and
progesterone
l two gonadotropic peptide hormones secreted by the anterior pituitary
gland — FSH (follicle stimulating hormone) and LH (luteinising hormone).
First half of the cycle

During the first half of each cycle, the dominant gonadotropin is FSH. This
stimulates secondary follicles in the ovary to grow in size and number. The
granulosa cells of the secondary follicles secrete oestrogen, which is the
dominant steroid hormone in this stage of the cycle. This affects:
l the oviduct, causing the development of more, larger and more active
cilia on the cells lining its walls. It also increases the secretion of more
glycoprotein; these changes prepare the oviduct for the arrival of an oocyte,
which will be moved along the oviduct by the cilia.
l the endometrium (lining of the uterus), causing the cells to divide to form a
thicker layer, ready to receive an embryo if the egg is fertilised
Mid-point of the cycle

At day 13 or 14, a surge in LH causes the primary oocyte in a single ovarian
follicle to complete meiosis I and continue to metaphase of meiosis II. It also
causes the follicle to shed the secondary oocyte into the oviduct. This is
ovulation.
Second half of the cycle

The LH surge also causes the granulosa cells lining the secondary follicles to
change to luteal cells, and switch to secreting largely progesterone and less
oestrogen.
Progesterone becomes the dominant steroid hormone from 5 days after
ovulation (which is when a fertilised embryo would enter the uterus).
Progesterone causes the cells in the endometrium to differentiate to form a
thick, vascularised layer. If fertilisation does not occur, the corpus luteum begins
to degenerate from day 23, so the secretion of progesterone also decreases. This
causes the breakdown of the endometrium, leading to the start of menstruation
on day 0 of the start of the next cycle (Figure 15.11).
Menstruation Ovulation
Hormone
level
LH
FSH
Oestrogen
Hormone
level
Progesterone
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28
Days of the menstrual cycle
Figure 15.11 Changes in hormones during the menstrual cycle

Negative feedback in the menstrual cycle
High concentrations of oestrogen inhibit the secretion of FSH and LH by the
anterior pituitary gland. This happens during the first half of the menstrual cycle,
causing the levels of FSH and LH to fall. However, when oestrogen levels are very Expert tip
high, a surge of LH secretion occurs, which brings about ovulation. Remember to say that FSH and LH are
Towards the end of the cycle, as oestrogen and progesterone levels fall, the produced by the anterior pituitary
gland. ADH is produced by the
inhibition of FSH and LH secretion is lifted, so the concentration of these two posterior pituitary gland.
hormones begins to increase, leading to the start of a new cycle.
The contraceptive pill

Most contraceptive pills contain synthetic hormones similar to progesterone Revision activity
and oestrogen. These prevent the secretion of LH and FSH from the anterior
l Convert the written description of
pituitary gland. There is therefore no development of secondary follicles in the the menstrual cycle on pp. 134–135
ovary (which is caused by FSH) and no ovulation (which is caused by a surge to a flow diagram.
of LH).
Table 15.1 Comparison of the mammalian nervous and endocrine systems
Feature Nervous system Endocrine system

Components Made up of neurones in Made up of secretory cells
nerves, brain and spinal in endocrine glands
cord
How information As electrical impulses in As chemicals (hormones)
is transmitted neurones carried in blood plasma
Speed of Very fast Slower
transmission
Duration of effect Often very brief Often longer term
Breadth of effect Often affects only a few Often affects several
effector cells different target organs
Control and coordination

in plants
As in animals, it is essential that cells in a plant can communicate with each
other. This allows them to coordinate their activities appropriately, and allows
the entire plant to respond to changes in its internal or external environment.
As in animals, plant communication systems involve receptors and effectors.
Plants have a system of electrical communication similar to that of an animal’s
nervous system, but ‘action potentials’ travel only very slowly and are very weak.
Venus fly trap Revised
The Venus fly trap (Figure 15.12) grows in soils that are low in nitrate. In order to
obtain sufficient nitrogen for protein synthesis, the Venus fly trap digests insects
and absorbs nutrients from them.
Modified leaf Position of nectar-secreting

glands
Sensory hairs
Glandular tissue that
secretes digestive enzymes
Figure 15.12 Venus fly trap

Nectar attracts insects such as flies to the modified leaf. The insect’s movements
cause the sensory hairs to move, and this stimulus causes calcium ion channels
in cells at the base of the hair to open. Calcium ions flow in, depolarising the
cells. If two or more hairs are stimulated, or if the same hair is stimulated twice
in quick succession, this depolarisation is great enough to trigger an action
potential.
The action potential travels across the leaf, and causes the lobes to change into
a convex shape, which makes them fold together and close the trap. The stiff
spikes on the outer surface of the trap slot together, sealing the trap.
Hydrolytic enzymes are then secreted, and these digest the prey to soluble
substances such as amino acids, which are absorbed into the leaf.
Plant growth regulators Revised
Like animal hormones, plant growth regulators are chemicals that act on target
cells, where they bind with receptors either on the cell surface membrane or Plant growth regulators are
inside the cell, and bring about changes. Unlike animal hormones, plant growth hormone-like substances that are
regulators are not made in specialised glands. produced in a plant and have effects
on target cells that carry receptors for
Many different plant growth regulators have been discovered, but there is still them.
much that we do not know about their actions. They often have different
effects at different concentrations, in different parts of a plant, at different stages
of its life cycle or when other hormones are present. Now test yourself
Auxins 5 Construct a table to compare plant
Auxins are a group of plant hormones that are produced by cells in regions of growth regulators with animal
cell division, or meristems. In a plant shoot, the apical bud (the bud at the hormones.
tip of the growing shoot) contains a meristem. Auxin is constantly made here. Answer on p.204
Auxin molecules are then moved down the shoot from cell to cell, through Tested
special auxin transporter proteins in the cell surface membranes.
Auxin has many different effects in plants, one of which is to stimulate
elongation of cells, which results in growth. It does this by stimulating proton
pumps in cell surface membranes to remove protons from the cytoplasm and
deposit them in the cell walls. This acidification of the cell walls loosens the
bonds between the cellulose molecules and matrix, allowing the cell to elongate
as it absorbs water.
Gibberellins
Gibberellin, also known as GA, is produced in the embryos of germinating
seeds, including wheat and barley, after they have absorbed water. GA switches
on several genes that encode enzymes that hydrolyse food reserves. (See also
p. 153.) These include starch, protein and lipid that are stored in the endosperm
of the seed. For example, amylase is produced, which hydrolyses starch to
maltose. The soluble maltose is transported to the embryo and used as an
energy source and a raw material for the production of new cells.
Now test yourself
GA also stimulates stem elongation, by causing cell division and cell elongation.
Some plants lack the last enzyme in the metabolic pathway by which gibberellin 6 Explain why applying gibberellin to
is synthesised, and they remain small. The gene for this enzyme has two alleles, plants with the genotype LeLe does
Le and le. Allele Le is dominant and codes for the enzyme. Plants homozygous not make them grow taller.
for le do not produce the enzyme. Applying gibberellin to these genetically Answer on p.204
dwarf plants makes them grow tall. Tested
Abscisic acid
See p. 127 for the role of abscisic acid in stomatal closure.

16 Inherited change
Meiosis Expert tip

Take great care to spell ‘meiosis’
correctly, so that it cannot be confused
A few cells in the human body — some of those in the testes and ovaries — are with ‘mitosis’. You will not be given
able to divide by a type of division called meiosis. Meiosis involves two divisions benefit of doubt if the examiner is not
(not one as in mitosis), so four daughter cells are formed. Meiosis produces four sure which one you mean.
new cells with:
l only half the number of chromosomes as the parent cell
l different combinations of alleles from each other and from the parent cell Meiosis is a type of cell division in
which a diploid cell divides to form
In animals and flowering plants, meiosis produces gametes. four haploid cells, in which different
In a human, body cells are diploid, containing two complete sets of combinations of alleles are present.
chromosomes. Meiosis produces gametes that are haploid, containing one
complete set of chromosomes. This is necessary so that the cell formed at Typical mistake
fertilisation (the zygote) is diploid.
Do not say that meiosis takes place
‘in’ gametes. It takes place in cells that
produce gametes.
Gametogenesis in mammals Revised
Gametogenesis is the production of haploid gametes from diploid somatic

(body) cells.
In mammals, the male gametes are spermatozoa (sperm) and the female
gametes are ova. Spermatogenesis takes place in the testes, and oogenesis in
the ovaries.
Spermatogenesis
n stands for the number of
Diploid spermatogonia at the edge of the seminiferous tubule (Figure 16.1)
chromosomes in a complete set. A cell
undergo mitosis and then meiosis to produce haploid spermatids. As meiosis with one set (n) is haploid. A cell with
proceeds, the cells move towards the centre of the tubule. The whole process two sets (2n) is diploid.
takes about 64 days (Figure 16.2).
Seminiferous tubule
Sertoli cell lining the tubule; these provide structural and
metabolic support for the developing spermatocytes and
spermatids, and secrete the fluid in the lumen of the testis
Spermatozoon (n)
Spermatid (n)
Secondary spermatocyte (cell in second division of

meiosis) — rarely visible as this stage is very short-lived
Primary spermatocyte (cell in first division of meiosis)
Spermatogonia (2n)
Figure 16.1 Histology of the testis
Inherited change 137

16 Inherited change
Mitosis Meiosis
First division Second division
Differentiation
Primary Secondary Spermatids Spermatozoans

spermatocyte spermatocytes (n) (n)
Spermatogonia (2n) (2n) (n)
Figure 16.2 Sequence of spermatogenesis
Oogenesis
Diploid oogonia divide by mitosis and then meiosis to produce haploid
secondary oocytes. At birth, the oogonia have already become primary oocytes,
in the first division of meiosis. The primary oocytes are inside primordial follicles
(Figure 16.3), in which the oocyte is surrounded by a layer of granulosa cells. The
primordial follicle remains in this state for many years.
At puberty, some of the primordial follicles develop into primary follicles, in
which the primary oocyte grows larger and develops a coat called the zona
pellucida. The primary follicle then develops into a secondary follicle, containing
extra layers of granulosa cells, which are surrounded by a theca.
From puberty onwards, some of the primary follicles develop into ovarian
follicles. Just before ovulation, the primary oocyte completes the first division of
meiosis to produce a secondary oocyte and a tiny polar body. At ovulation, the
secondary oocyte is in metaphase of the second division of meiosis (Figure 16.4).
Meiosis only completes after the egg is fertilised.
After ovulation, the remains of the ovarian follicle develop into a corpus luteum.
2 Secondary follicle
containing primary
oocyte
3 Ovarian follicle.
Just before
ovulation the
primary oocyte
becomes a
1 Primary follicle secondary oocyte
containing primary Ovulation
oocyte Corpus luteum
Figure 16.3 Histology of an ovary
Mitosis Meiosis
First division Second division
Polar body
Polar body
Primary oocyte
Secondary oocyte Ovum (n) Revision activity
(before puberty)
Oogonia (2n) (after puberty) (n)
(2n) l Construct a table to compare
oogenesis and spermatogenesis.
Figure 16.4 Sequence of oogenesis

Gametogenesis in flowering plants
16 Inherited change
Revised
In flowering plants, the male gametes are nuclei inside pollen grains, which are Typical mistake
made in the anthers of a flower. The female gametes are nuclei inside embryo Pollen grains are not male gametes.
sacs, which are made in the ovules inside the ovaries of a flower. They contain the male gametes.
Production of male gametes

Inside the anthers, pollen mother cells divide by meiosis to form four haploid
cells. The nuclei in each of these haploid cells divide by mitosis to produce two
haploid nuclei in each cell (Figure 16.5). These cells mature into pollen grains.
One of the nuclei is the male gamete nucleus, which can fuse with a female
nucleus in an embryo sac to produce a diploid zygote. This zygote will grow into
an embryo plant.
Meiosis Mitosis
Pollen mother Four haploid Young pollen grains Mature pollen

cell (2n) cells (n) containing two grains (n)
haploid nuclei (n)
Figure 16.5 Production of male gametes in a flowering plant
Production of female gametes

Inside the ovary of a flower are one or more ovules. Inside the ovule, a diploid
embyro sac mother cell divides by meiosis to form four haploid cells. Only one
of these continues to develop (Figure 16.6). Its haploid nucleus divides by mitosis
three times, forming a total of four haploid nuclei inside a structure called an
embryo sac. One of these nuclei is the female gamete nucleus (in the egg
cell) which can be fertilised by a male gamete nucleus from a pollen grain.
Meiosis Mitosis (3 divisions)

Egg cell (n)
Two synergid cells (n)

Endosperm nucleus (2n)
Three antipodal cells (n)
Diploid spore Mature embryo sac

mother cell (2n)
Figure 16.6 Production of a female gamete in a flowering plant
Behaviour of chromosomes during meiosis Revised
Before meiosis begins, DNA replication takes place exactly as it does before
Homologous chromosomes are
mitosis (p. 40). However, in the early stages of meiosis homologous
chromosomes that carry the same
chromosomes (the two ‘matching’ chromosomes in a nucleus) pair up.
genes (though not necessarily the
Figure 16.7 shows how meiosis takes place. same alleles of the genes) in the same
position.

16 Inherited change
Prophase I Metaphase I
● Centrioles divide and are ● The spindle continues to ● The nuclear envelope
involved in spindle formation. form. disappears.
● Chromosomes beginning to ● Chromosomes pair and are ● The bivalents are arranged
condense. now visible as bivalents. on the equator.
Anaphase I Telophase I and cytokinesis
● Chromosomes partly unwind

● Homologous chromosomes separate to
● Spindle disappears and the nuclear envelopes form
opposite poles, pulled by spindle fibres.
● The cell divides
Prophase II Metaphase II
● Centrioles divide and spindles form ● Individual chromosomes line up on equator.

● Chromosomes condense
Anaphase II Telophase II
● Centromeres split
● Chromatids move apart, pulled by
spindle fibres ● Spindles disappear
● Nuclear envelopes appear
● Chromosomes unwind
Figure 16.7 Meiosis
How meiosis causes genetic variation Revised
Meiosis produces genetic variation. This is done by

l crossing over
l independent assortment
Further genetic variation is produced when gametes produced by meiosis fuse

together to produce a zygote.

16 Inherited change
Crossing over
Each chromosome in a homologous pair carries genes for the same
characteristics at the same locus. The alleles of the genes on the two
chromosomes may be the same or different.
During prophase of meiosis I, as the two homologous chromosomes lie side by
side, their chromatids form links called chiasmata (singular: chiasma) with each
other. When they move apart, a piece of chromatid from one chromosome may
swap places with a piece from the other chromosome. This is called crossing
over. It results in each chromosome having different combinations of alleles than
it did before (Figure 16.8).
Homologous Pieces of each
pair of chromatid have
chromosomes swapped places,
resulting in new
combinations of
alleles
Chiasma
Figure 16.8 Crossing over in meiosis
Independent assortment
Another feature of meiosis that results in the shuffling of alleles — and therefore
genetic variation — is independent assortment. During the first division of
meiosis, the pairs of homologous chromosomes line up on the equator before
being pulled to opposite ends of the cell. Each pair behaves independently from
every other pair, so there are many different combinations that can end up
together. Figure 16.9 shows the different combinations you can get with just two
pairs of chromosomes. In a human there are 23 pairs, so there is a huge number
of different possibilities.
Parent cell Homologous pair

of chromosomes
A a Bb Now test yourself

1 Many plants are hermaphrodites,
producing both male and female
Each pair of homologous chromosomes lines up at the equator gametes. Explain why self-
fertilisation is likely to produce
offspring with some genetic
The chromosomes ...or like this variation, but not as much as if
B b may line up like b B fertilisation involved gametes from
this... two different plants.
Answer on p.204
A a A a Tested
Combinations Combinations
AB and ab Ab and aB
A B a b a B
A b
A B a b A b a B
Figure 16.9 Independent assortment in meiosis

Genetics
16 Inherited change
Genes are passed from parents to offspring inside the nuclei of gametes. During
sexual reproduction, gametes fuse to produce a zygote, which contains one
set of chromosomes from each parent. The study of the passage of genes from
parent to offspring is called genetics.
Genes and alleles Revised
The two chromosomes in a diploid cell that are similar (e.g. the two
chromosome 1s) are said to be homologous. They each contain the same genes
in the same position, known as the locus of that gene. This means that there are
two copies of each gene in a diploid cell.
Genes often come in different forms. For example, the gene for a protein that An allele is one of two or more
forms a chloride transporter channel in cell surface membranes, called the CFTR different forms of a gene that codes for
protein, has a normal form and several different mutant forms. These different a particular polypeptide or protein.
forms of a gene are called alleles.
Homozygote and heterozygote

An organism that has two identical alleles for a particular gene is a homozygote.
An organism that has two different alleles for a particular gene is a
heterozygote.
Dominant and recessive

We can use letters to represent the different alleles of a gene. For example, we
could use F to represent the normal cystic fibrosis allele, and f to represent a
mutant allele.
There are three possible combinations of these alleles in a diploid organism: FF,
Ff or ff. These are the possible genotypes of the organism.
These different genotypes give rise to different phenotypes — the observable
characteristics of the organism.
Genotype Phenotype
FF normal
Ff normal
ff cystic fibrosis
A person with the genotype Ff is said to be a carrier for cystic fibrosis, because
they have the cystic fibrosis allele but do not have the condition.
The Ff genotype does not cause cystic fibrosis because the F allele is dominant
and the f allele is recessive. A dominant allele is one that is expressed (has an
effect) in a heterozygous organism. A recessive allele is one that is only expressed
when a dominant allele is not present.
l The dominant allele should always be symbolised by a capital letter, and the
recessive allele by a small letter. The same letter should be used for both (not
F and c, for example).
l If you are able to choose the symbols that you use in a genetics question,
then choose ones where the capital and small letter are different in shape, to
avoid confusion (not C and c, for example).

Monohybrid inheritance
16 Inherited change
Revised
This is the inheritance of a single gene. For example, albinism (the lack of melanin
in the skin) is caused by a recessive allele, a. Imagine that a man with the
genotype Aa and a woman with the genotype AA have children. In their testes
and ovaries, gametes are produced. In the man, half of his sperm will contain the
A allele and half will contain the a allele. All of the woman’s eggs will contain the
A allele.
We can predict the likely genotypes of any children that they have using a
genetic diagram. Genetic diagrams should always be set out like this:
Parents’ genotypes Aa × AA
Gametes’ genotypes A a A
Offspring genotypes and phenotypes
eggs
A
AA
A normal
sperm
Aa
a normal
We can therefore predict that there is an equal chance of any child born to
them having the genotype AA or Aa There is no chance they will have a child
with albinism.
If both parents have the genotype Aa:
Parents’ genotypes Aa × Aa
Gametes’ genotypes A a A a
eggs
A a
AA Aa
A normal normal
sperm
Aa aa
a normal albinism
We can therefore predict that, each time they have a child, there is a 1 in 4
chance that it will have the genotype aa and have albinism.
Notice:
l In the examination, it may be important to show the whole genetic diagram,
not just the Punnett square (the table showing the genotypes of the
offspring).
l The ‘gametes’ genotype’ line in the genetic diagram shows the different kinds
of gametes the parents can produce. If there is only one kind of gamete, you
only need to write down one kind — there is no need to write the same
one down twice. (If you do that, it will not make any difference to your final
answer, but will make twice as much work in writing it all down.)
l The gamete genotypes are shown with a circle drawn around them. This
is a convention — if you do this, the examiner will understand that they
represent gametes.

l There is often a mark for stating the phenotype produced by each genotype
16 Inherited change
among the offspring. The easiest and quickest way to do this is to write the
phenotypes in the boxes in the Punnett square.
l The genotypes inside the Punnett square show the chances or probabilities
of each genotype being produced. If you have four genotypes, as in the
example above, this does not mean there will be four offspring. It means
that, every time an offspring is produced, there is a 1 in 4 chance it will be
AA, a 1 in 4 chance it will be aa and a 2 in 4 (better written as 1 in 2) chance
it will be Aa.
l An alternative way of writing ‘a 1 in 4 chance’ is ‘a probability of 0.25’, or ‘a
25% probability’.
l You could also give the final answer in terms of expected ratios. For example, Expert tip
‘We would expect the ratio of unaffected offspring to offspring with It is tempting just to show the
albinism to be 3:1’. Punnett square, but it is almost always
important to include the complete
l The chance of any individual child inheriting a particular genotype is
genetic diagram in your answer in
unaffected by the genotype of any previous children. Each time a child is order to get full marks.
conceived the chances are the same, as shown in the genetic diagram above.
Test crosses
A test cross is a breeding experiment that is carried out to determine the Expert tip
genotype of an organism that shows the dominant characteristic. Test crosses used to be called
For example, in a species of mammal, the gene for hair colour has two alleles, backcrosses, so you may come across
this term in old textbooks.
B and b. Allele B gives brown fur and allele b gives white fur.
Genotype Phenotype
BB brown fur
Bb brown fur
bb white fur
If an animal has brown fur, we do not know if its genotype is BB or Bb. We

can find out by breeding it with an animal with white fur, whose genotype
must be bb.
If there are any white offspring, then the unknown animal must have the
genotype Bb, as it must have given a b allele to these offspring.
If there are no white offspring, then the unknown animal probably has the
genotype BB. However, it is still possible that it is Bb and, just by chance, none
of its offspring inherited the b allele from it.
Codominance
In the albinism example, one allele is dominant and the other recessive. Some
alleles, however, are codominant. Each allele has an effect in a heterozygote.
For codominant alleles, it is not correct to use a capital and small letter to
represent them. Instead, a capital letter is used to represent the gene, and a
superscript to represent the allele.
For example, in some breeds of cattle there are two alleles for coat colour: Expert tip
CR is the allele for red coat It is tempting not to bother writing
allele symbols with superscripts
CW is the allele for white coat because it is much quicker just to use a
single letter, but it is very important to
Genotype Phenotype do it correctly.
CRCR red coat
C R CW coat with a mixture of red
and white hairs — roan
CW CW white coat

The inheritance of codominant alleles is shown using a genetic diagram just like
16 Inherited change
the one on p. 143. You might like to try showing that two roan cattle would be
expected to have offspring with roan, red and white coats in the ratio 2:1:1.
F1 and F2 generations Typical mistake

When two organisms that are homozygous for two different alleles of a gene,
F1 cannot be used for the first set of
for example AA and aa, are crossed, the offspring produced are called the offspring from any cross. You should
F1 generation. The F1 are all heterozygous, i.e. Aa. not use it unless the offspring are
the result of a cross between two
When two of the F1 generation are crossed, their offspring are called the homozygous parents.
F2 generation.
Multiple alleles
Many genes have more than two alleles. For example, the gene that determines
the antigen on red blood cells, and therefore your blood group, has three alleles
— IA, IB and Io.
IA and IB are codominant. They are both dominant to Io, which is recessive.
Genotype Phenotype
IAIA blood group A
IAI B blood group AB
IAI o blood group A
IBIB blood group B
IBIo blood group B Expert tip
IoIo blood group O Note that you should show the
genotypes correctly, using the I
Genetic diagrams involving multiple alleles are constructed in the same way as symbols with their superscripts. The
A and B symbols represent the blood
before. For example, you could be asked to use a genetic diagram to show how group, not the genotype.
parents with blood groups A and B could have a child with blood group O.
Parents’ phenotypes blood group A × blood group B
Parents’ genotypes IAIo IB Io
Gametes’ genotypes IA Io IB Io
IA Io
IAIB IB Io
IB Blood group AB Blood group B
IAIo IoIo
Io Blood group A Blood group O
When solving a problem like this:

l Begin by working out and then writing down a table of genotypes and
phenotypes, which you can easily refer back to as you work through
the problem.
l Consider whether you can tell the genotype of any of the individuals in the
problem from their phenotype. Here, we know that the child with blood Now test yourself
group O must have the genotype IoIo. 2 Construct a genetic diagram
l Work back from there to determine the genotypes of the parents. In this to predict the genotypes and
case, each of them must have had an Io allele to give to this child. phenotypes of the children of two
l Always show a complete genetic diagram. Do not take short cuts, even if people with blood group AB.
you can see the answer straight away, as there will be marks for showing Answer on p.204
each of the steps in the diagram. Tested

16 Inherited change
Sex linkage
In a human cell, there are two sex chromosomes. A woman has two X
A sex-linked gene is one that is
chromosomes. A man has an X chromosome and a Y chromosome.
present on the X chromosome but not
The X chromosome is longer than the Y chromosome. Most of the genes on on the Y chromosome.
the X chromosome are not present on the Y chromosome. These are called sex-
linked genes.
For example, a gene that determines the production of red-receptive and green-
receptive pigments in the retina of the eye is found on the X chromosome.
There is a recessive allele of this gene that results in red-green colour-blindness.
A woman has two copies of this gene, because she has two X chromosomes. A
man has only one copy, because he has only one X chromosome.
If the normal allele is A, and the recessive abnormal allele is a, then these are the
possible genotypes and phenotypes a person may have:
Genotype Phenotype
XA XA female with normal vision
X A Xa female with normal vision
Xa Xa female with colour blindness
XA Y male with normal vision
Xa Y male with colour blindness
Notice:
l Sex-linked genes are shown by writing the symbol for the allele as a
superscript above the symbol for the X chromosome.
l There are three possible genotypes for a female, but only two possible
genotypes for a male.
For example, you could be asked to predict the chance of a woman who is a
carrier for colour blindness (that is, heterozygous) and a man with normal vision
having a colour-blind child.
Parents’ phenotypes woman with normal vision × man with normal vision
Parents’ genotypes X A Xa XA Y
Gametes’ genotypes XA Xa XA Y
XA Xa
XA XA X A Xa
XA Female with Female with
normal vision normal vision
XA Y Xa Y
Y Male with Colour-blind
normal vision male
There is therefore a 1 in 4 chance that a child born to this couple will be

a colour-blind boy. There is a 1 in 2 chance of any boy that is born being
colour-blind.

Notice:
16 Inherited change
l Always show the X and Y chromosomes when working with sex-linked
genes.
l A boy cannot inherit a sex-linked gene from his father, because he only
gets a Y chromosome from his father. His X chromosome (which carries
sex-linked genes) comes from his mother. Now test yourself
Another example of sex linkage in humans is haemophilia. This is caused by 3 Explain why a man who is colour-
a recessive allele of a gene for a blood-clotting factor, carried on the X blind cannot pass this condition to
chromosome. In males who have this recessive allele, blood clotting does not his son.
take place normally, so wounds can bleed copiously, and internal bleeding can Answer on p.204
take place, for example into joints. Tested
Dihybrid inheritance Revised
This involves the inheritance of two genes. For example, a breed of dog may
have genes for hair colour and leg length.
Allele A is dominant and gives brown hair. Allele a is recessive and gives
black hair.
Allele L is dominant and gives long legs. Allele l is recessive and gives
short legs.
As before, always begin by writing down all the possible genotypes and
phenotypes.
Genotype Phenotype
AALL brown hair, long legs
AaLL brown hair, long legs
aaLL black hair, long legs
AALl brown hair, long legs
AaLl brown hair, long legs
aaLl black hair, long legs
AAll brown hair, short legs
Aall brown hair, short legs
aall black hair, short legs
Notice:
l Always write the two alleles of one gene together. Do not mix up alleles of
the two different genes.
l Always write the alleles in the same order. Here, we have decided to write
the alleles for hair colour first, followed by the alleles for leg length. Do not
swap these round part-way through.
A dihybrid cross
A genetic diagram showing a dihybrid cross is set out exactly as for a
monohybrid cross. The only difference is that there will be more different types
of gamete. Each gamete will contain just one allele of each gene.
This genetic diagram shows the offspring we would expect from a cross
between two dogs that are both heterozygous for both genes.

Parents’ phenotypes brown hair, long legs × brown hair, long legs
16 Inherited change
Parents’ genotypes AaLl AaLl

Gametes’ genotypes AL Al aL al AL Al aL al
AL Al aL al
AL AALL AALl AaLL AaLl

brown, long brown, long brown, long brown, long
Al AALl AAll AaLl Aall
brown, long brown, short brown, long brown, short
aL AaLL AaLl aaLL aaLl
brown, long brown, long black, long black, long
al AaLl Aall aaLl aall
brown, long brown, short black, long black, short
We would therefore expect offspring in the ratio 9 brown hair, long legs : 3 Now test yourself
brown hair, short legs : 3 black hair, long legs : 1 black hair, short legs.
4 Use a genetic diagram to show
Notice: that, when a homozygous recessive
l Each gamete contains one allele of each gene (one of either A or a, and one individual is crossed with one that
of either L or l). is heterozygous for both genes, the
l The ratio 9:3:3:1 is typical of a dihybrid cross between two heterozygotes, expected ratio would be 1:1:1:1.
where the alleles of both genes show dominance (as opposed to Answer on p.204
codominance). Tested
Autosomal linkage
If two genes are on the same chromosome, then their alleles do not assort
independently during meiosis. The alleles on the same chromosome tend to be Autosomal linkage is the tendency
of two characteristics to be inherited
inherited together. This is called autosomal linkage (Figure 16.10).
together, because the genes that
For example, imagine that in a species of animal, the gene for eye colour (E/e) cause them are on the same (non-sex)
and the gene for fur colour (F/f) are on chromosome 3. A male animal has the chromosome.
genotype EeFf. In his cells, one of his chromosome 3s carries the E and F alleles,
and the other has the e and f alleles.
E e
F f
Figure 16.10 Autosomal linkage
When meiosis takes place in the male parent’s testes, most of the sperm
produced will either have the genotype EF or ef. Only a very small number,
produced when crossing over took place between the two gene loci, will have
the genotype Ef or eF.
Now imagine that this male mates with a female with the genotype eeff. All of
her eggs have the genotype ef.

Parents’ phenotypes brown eyes, dark fur × blue eyes, pale fur
16 Inherited change
Parents’ genotypes EeFf eeff
Gametes’ genotypes EF Ef eF ef ef
EF Ef eF ef Now test yourself
ef EeFf Eeff eeFf eefff 5 Construct a genetic diagram
brown, dark brown, pale blue, dark blue, pale to predict the genotypes and
phenotypes of the offspring if
If there was no autosomal linkage, we would expect offspring genotypes in the the male with genotype EeFf is
ratio 1:1:1:1. However, because the genes are linked together, we find that most mated with a female with the same
of the offspring resemble their two parents. Only a small number (perhaps none genotype.
at all) show different combinations of eye colour and fur colour. These few are Answer on p.205
called recombinants. Tested
Using statistics in genetics Revised
Genetic diagrams can be used to tell us the probabilities of particular genotypes

occurring in offspring. However, because these are just probabilities, the actual
phenotypic ratios are rarely exactly as we predicted.
For example, imagine that a plant has genes for flower colour (allele Y for yellow
and y for white flowers) and petal size (P for large petals and p for small petals).
We cross two plants that are heterozygous for both alleles. If these alleles are
not linked (i.e. the genes are on different chromosomes) we would expect to get
offspring with phenotypes:
yellow, large yellow, small white, large white, small
9 : 3 : 3 : 1 Now test yourself
However, what we actually get are 847 offspring with these phenotypes: 6 Construct two genetic diagrams
to show the expected offspring
yellow, large yellow, small white, large white, small ratios from this cross (a) if the
489 : 151 : 159 : 48 genes are linked with YP on one
chromosome and yp on the other,
What we would expect to have got is: and (b) if the genes are not linked.
yellow, large yellow, small white, large white, small Answer on p.205
476 : 159 : 159 : 53 Tested
The question we need to ask is: are these results close enough to the expected
results to indicate that we are right in thinking that the two genes are not
linked? Or are they so different from the results we expected that they indicate
that something different is happening? In other words, is the difference between
our observed and expected results significant?
We can use statistics to tell us how likely it is that the difference between our
observed results and our expected results is just due to chance, or whether it is
so different that we must have been wrong in our predictions.
Null hypothesis and probability level

A null hypothesis is a statement that
A statistics test begins by setting up a null hypothesis. We then use the
there is no relationship between two
statistics test to determine the probability of the null hypothesis being true. variables, or that the results we have
In this case, our null hypothesis would be: obtained show no significant difference.
The observed results are not significantly different from the expected results.
We then work through a statistics test, which gives us a probability of our null
hypothesis being correct. In biology, it is conventional to say that:

l if the probability of the null hypothesis being correct is greater than or equal
16 Inherited change
to 0.05, then we can accept the null hypothesis.

l if the probability is less than 0.05 that the null hypothesis is correct, then we
must reject it
The chi-squared test

To test the possibility of our null hypothesis being correct, the most suitable
statistical test for this set of results is the chi-squared test. This can also be
written as χ2 test.
l Construct a table similar to the one below. You need one column for each
category of your results.
Yellow, large Yellow, small White, large White, small
Observed
numbers, O
Expected
numbers, E
O−E
(O − E)2
(O − E)2
E
2
Σ (O − E)
E
l Fill in the observed numbers and the expected numbers for each category.
l Calculate O − E for each category.
l Calculate (O − E)2 for each category.
(O − E)2
l Calculate for each category.
E
(O − E)2 (O − E)2
l Add together all of the values, to give you Σ .
E E
Your table now looks like this:
Yellow, large Yellow, small White, large White, small
Observed 489 151 159 48
numbers, O
Expected 476 159 159 53
numbers, E
O−E 13 −8 0 −5
(O − E)2 169 64 0 25
(O − E)2 0.36 0.40 0 0.47
E
2
Σ (O − E) = 1.23
E
The number 1.23 is the chi-squared value.

You now need to look this up in a probability table, to find out what it tells us
about the probability of the null hypothesis being correct.
This is a part of a probability table for chi-squared values.
Degrees of Probability of null hypothesis being correct

freedom 0.1 0.05 0.01 0.001
1 2.71 3.84 6.64 10.83
2 4.60 5.99 9.21 13.82
3 6.25 7.82 11.34 16.27
4 7.78 9.49 13.28 18.46

The numbers inside the cells in the table are chi-squared values.
16 Inherited change
The numbers in the first column are degrees of freedom. You have to choose
the correct row in the table for the number of degrees of freedom in your data.
In general:
degrees of freedom = number of different categories − 1 Now test yourself
In this case, there were four categories (the four different phenotypes), so there 7 A species of mammal has either
are 3 degrees of freedom. white or grey fur, and either a
l Look along the row for 3 degrees of freedom until you find the number long or short tail. Several crosses
closest to the chi-squared value you have calculated. This was 1.23, and all between a heterozygous animal
the numbers in the row are much bigger than this. The closest is 6.25. with white fur and long tail, and an
animal with grey fur and short tail,
l Look at the probability associated with this number. It is 0.1. This means produced these offspring:
that if the chi-squared value was 6.25, there is a 0.1 probability that the null white fur, long tail 12
hypothesis is correct. white fur, short tail 2
l Remember that, if the probability of the null hypothesis being correct is grey fur, long tail 2
equal to or greater than 0.05, you can accept it as being correct. 0.1 is much grey fur, short tail 12
bigger than 0.05, so you can definitely accept the null hypothesis as being Use the chi-squared test to
correct. Indeed, because your value of chi-squared was actually much smaller determine whether these results
than 6.25, we would need to go a long way further left in the table, which show that the genes for fur colour
would take us into probabilities even greater than 0.1. and eye colour are on the same
l We can therefore say that the difference between the observed results and chromosome.
expected results is not significant. The differences between them are just due Answer on p.205
to random chance. Tested
Gene interactions
Sometimes, two genes at different loci interact to produce a phenotype. For
example, in mice, two genes affect fur colour:
l Gene for distribution of melanin in hairs: A produces banding (agouti),
a produces a uniform distribution.
l Gene for presence of melanin in hairs: B produces melanin, b does not
produce melanin.
Clearly, gene A/a can only have an effect if the pigment melanin is present. If
there is no melanin, fur colour is white.
Genotype Phenotype
AABB agouti Now test yourself
AaBB agouti 8 Construct a genetic diagram to
aaBB black predict the ratios of phenotypes
resulting from a cross between two
AABb agouti mice with the genotypes AaBB and
AaBb agouti AaBb.
aaBb black 9 How could you use a test cross to
find out the genotype of a white
AAbb white
mouse?
Aabb white
aabb white
Answers on p.206
Tested
Gene mutation Revised
A mutation is a random, unpredictable change in the DNA in a cell. Gene

mutation occurs when there is a change in the sequence of bases in one part
of a DNA molecule. This can occur by substitution, deletion or insertion
(Figure 16.11).

16 Inherited change
Original DNA
A T G C C G A C T ...
After addition After deletion After substitution

A T T G C C G A C T ... A T C C G A C T ... A T C C C G A C T ...
Figure 16.11 Types of gene mutation
Deletion or addition of a base causes all of the following DNA triplets to be
different; this is called a frame shift. Frame shifts have a major effect during
protein synthesis, because a completely new sequence of amino acids will be
used. One of the new triplets could even be a ‘stop’ code. Substitution, however, Typical mistake
may have no effect at all, because only one triplet is altered. This may code for Students often say that mutation
the same amino acid as the original one. Even if it codes for a different one, only is most likely to happen during
meiosis. This is not correct; genes are
that amino acid is changed, and the protein that is made may still be able to most likely to mutate during DNA
function normally. replication. Meiosis does produce
Mutations are most likely to occur during DNA replication, for example when a variation, but this is by reshuffling
genes, not by producing new ones by
‘wrong’ base might slot into position in the new strand being built. Almost all mutation.
these mistakes are repaired immediately by enzymes, but some may persist.
Conditions resulting from mutations in humans include the following:
l Sickle cell anaemia — results from a substitution in the gene coding for
the β polypeptide in a haemoglobin molecule. You can find more about this
on p. 156.
l Haemophilia — results from a mutation in the gene coding for a clotting
factor, factor VIII. The mutant allele is recessive. This gene is found on the X
chromosome, so haemophilia is a sex-linked condition.
l Albinism — results from one of several different mutations in a gene
coding for melanin production. The mutant allele is recessive, and does not
allow melanin to be produced. Mammals homozygous for such an allele
have no melanin in their coats or irises, and are white with pink eyes. There
are several different mutant alleles that can have this effect. One of them
prevents the formation of the enzyme tyrosinase, which is involved in the
conversion of the amino acid tyrosine to melanin.
tyrosinase
tyrosine → DOPA → dopaquinone → melanin
l Huntington’s disease — results from a mutation in the gene coding for
a protein called huntingtin. The mutant allele is dominant, and affects
neurones in the brain. These effects are usually not seen until the person is
between 35 and 44 years old, when they begin to develop problems with
muscle coordination and cognitive difficulties.
Control of gene expression Gene expression is the transcription

All the cells in your body contain a full set of genes. However, in each cell only a
and translation of the gene, resulting
certain set of these genes is used to make proteins (expressed). Different genes in the production of the polypeptide or
are expressed in different cells, and at different times. protein for which it codes.
The lac operon Revised
One of the best-studied examples of the control of gene expression is the gene
for the production of the enzyme lactase (β galactosidase) in the bacterium
Escherichia coli. The bacterium only produces lactase when the sugar lactose is
present. The gene for this enzyme is closely associated with several other regions
of DNA that control whether or not the gene is used to make lactase.

The stretch of DNA that codes for the production of the enzyme is called the
16 Inherited change
lac operon (Figure 16.12). It is made up of:
l three structural genes, which code for proteins involved in producing
the enzyme
l a promoter that allows transcription of the structural genes to begin
l an operator that allows a molecule called a transcription factor to bind,
which in this case stops the structural genes being transcribed
Part of the bacterium’s DNA
lac operon
Structural genes
lacZ lacY lacA
Promoter for Regulatory Promoter for Operator

regulatory gene gene structural genes
Figure 16.12 The lac operon
Alongside the lac operon are two other regions of DNA that control the
expression of the structural genes:
l a regulatory gene, which codes for a protein that acts as a repressor
l a promotor for the regulatory gene, which allows its transcription to begin
When there is no lactose in the medium in which the bacterium is growing, the
regulatory gene is expressed and therefore the repressor protein is produced.
This repressor protein binds to the operator region of the lac operon. This
prevents RNA polymerase binding to the promoter for the structural genes, so Now test yourself
they are not transcribed and no lactase is made.
10 Suggest the advantage to the
When there is lactose in the medium, it binds to the repressor protein and alters bacterium of producing lactase
its shape. This prevents the repressor protein from binding to the operator only when lactose is present.
region. Now RNA polymerase can bind to the promoter, and the structural Answer on p.206
genes can be transcribed. Lactase is made. Tested
Inducible and repressible enzymes

Lactase is said to be an inducible enzyme. This means that its production only
occurs when its substrate is present. The presence of the substrate prevents the
repressor binding with the operator, so the presence of the substrate induces
(causes) production of the enzyme. Other enzymes might be repressible
enzymes. This means that the presence of a particular substance allows the
repressor to bind with the operator and stops the enzyme being produced.
Transcription factors in eukaryotes Revised
A transcription factor is a molecule that binds to DNA and causes or prevents the
transcription of a gene. We have seen that transcription factors help to control
gene expression in bacteria (prokaryotes), and they also do so in eukaryotes.
For example, production of the enzyme amylase in germinating seeds depends
on a transcription factor called PIF (phytochrome interacting protein) binding
with the promoter region next to the gene that codes for amylase.
l When no gibberellin is present, PIF is bound to a protein called a DELLA
protein. This prevents PIF binding with the promoter for the amylase gene,
so no amylase is made.
l When gibberellin is present, it binds with a receptor and an enzyme. This
activates the enzyme, which breaks down the DELLA protein. PIF is now free Revision activity
to bind to the promoter for the amylase enzyme, and amylase is produced. l Construct a simple diagram
showing how gibberellin promotes
This explains how gibberellin causes amylase production during seed the synthesis of amylase.
germination, described on p. 136.

Variation
Differences between the phenotypes of individuals belonging to the same
species are known as variation. Variation can be continuous or discontinuous.
l Continuous variation occurs when a feature can have any value between
two extremes, such as height and mass in humans.
l Discontinuous variation occurs when a feature has only a few distinct
categories, such as blood groups in humans.
In general, continuous variation is caused when the effects of many different
genes act together to influence a characteristic. The environment may also have
an effect. Discontinuous variation is caused by one or a few genes that control
the characteristic, with little or no influence from the environment.
Effect of the environment on phenotype Revised
Hair colour in cats

Many different genes determine hair colour in cats. At least eight different genes,
at different loci, are known to influence hair colour and it is thought that there
are probably more. These are known as polygenes. Depending on the particular
combination of alleles that a cat has for each of these genes, it can have any of
a very wide range of colours. Hair colour in cats is an example of continuous
variation. There is a continuous range of variation in colour between the very
lightest and very darkest extremes.
The cat hair colour genes exert their effect by coding for the production of
enzymes. One such gene is found at the C locus. Siamese cats have two copies
of a recessive allele of this gene called cs. This gene codes for an enzyme that
is sensitive to temperature. It produces dark hair at the extremities of paws,
ears and tail where the temperature is lower, and light hair in warmer parts of
the body. The colouring of a Siamese cat is therefore the result of interaction
between genes and environment.
Height
Human height is also affected by many different genes at different loci. It is also
strongly affected by environment. Even if a person inherits alleles of these genes
that give the potential to grow tall, he or she will not grow tall unless the diet
supplies plenty of nutrients to allow this to happen. Poor nutrition, especially
in childhood, reduces the maximum height that is attained. A similar situation
occurs in plants, where individual plants with genes that allow tall growth may
remain stunted if they are growing in soil that is short of particular mineral ions
(e.g. nitrate) or if they do not get enough water.
Cancer
The risk of developing cancer is influenced by both genes and environment.
For example, a woman with particular alleles of the genes BRCA1 or BRCA2
has a 50–80% of chance of developing breast cancer at some stage in her life.
This is a much higher risk than for people who do not have these alleles. The
normal alleles of these genes protect cells from changes that could lead to them
becoming cancerous.

However, environment also affects this risk. Smoking, for example, increases the

risk even further. Taking the drug tamoxifen can reduce the risk.
The t-test Revised
You may be asked to use the t-test to determine whether the variation shown in
one population is significantly different from the variation in another population.
The t-test is described on pp. 186–188.
Natural and artificial selection

In a population of organisms, each parent can produce more young than are
required to maintain the population at a constant level. For example, each pair
of adult foxes in a population needs to have only two young in their lifetime to
keep the population the same size. However, a pair of foxes can have more than
30 young. The population can potentially overproduce. But the population
generally stays approximately the same size because most of the young do not
survive long enough to reproduce.
In a population, not every organism has exactly the same alleles or exactly the
same features. There will be genetic variation within the population. Those
organisms whose particular set of features is best suited to the environment are
most likely to survive. Those with less useful features are more likely to die.
The organisms with the most useful features are therefore more likely to reach
adulthood and reproduce. Their alleles will be passed on to their offspring. Over
many generations, the alleles that confer useful characteristics on an individual
are therefore likely to become more common in the population. Alleles that Natural selection is the increased
do not produce such useful characteristics are less likely to be passed on to chance of survival and reproduction of
successive generations and will become less common. organisms with particular phenotypes,
because they are better adapted to
This process is called natural selection. Over time, it ensures that the their environment than those with
individuals in a population have features that enable them to survive and other phenotypes.
reproduce in their environment.
Stabilising, directional and disruptive selection Revised
When the environment is fairly stable, natural selection is unlikely to bring

about change. If the organisms in a population are already well adapted to their
environment, then the most common alleles in the population will be those that
confer an advantage on the organisms, and it is these alleles that will continue to
be passed on to successive generations. This is called stabilising selection.
However, if the environment changes, alleles that were previously advantageous
may become disadvantageous. For example, in a snowy environment the
individuals in a species of mammal may have white fur that camouflages them
against the snow and confers an advantage in escaping predators. If the climate
changes so that snow no longer lies on the ground, then animals with white
fur may be more likely to be killed than animals with brown fur. Those with
brown fur are now most likely to reproduce and pass on their alleles to the next
generation. Over time, brown may become the most common fur colour in
the population. This is an example of directional selection or evolutionary
selection (Figure 17.1).
Directional selection may result in evolution. Evolution can be defined as a
long-term change in the characteristics of a species, or in the frequency of
particular alleles within the species.
Selection and evolution 155

In a snowy environment, white animals
are more camouflaged, making them less
visible to predators, so are at a selective
advantage. The white animal is most
likely to survive.
In an environment without snow, white

animals are very noticeable and more
likely to be preyed upon. The white animal
is least likely to survive.
Figure 17.1 Stabilising and directional selection Now test yourself
In some circumstances, two different phenotypes can each confer advantages. 1 Sketch a graph, like those showing
For example, in a population of snails, those with dark brown shells and those the results of stabilising and
with pale shells may each be well camouflaged, while those with mid brown directional selection, to show the
shells may not be camouflaged. In this case, selection will tend to remove most result of disruptive selection.
of the snails with mid-range colour from the population, resulting in two distinct Answer on p.206
colour ranges surviving. This is called disruptive selection. Tested
The founder effect and genetic drift Revised
Natural selection is not the only way in which allele frequencies can change in
a population. For example, imagine that just three lizards were washed away
from an island and drifted to another island where there were no lizards. These
three lizards would not contain all of the various alleles present in the original
population. The population that grew from these three colonisers would
therefore have different allele frequencies from the original population. This is
called the founder effect.
In small populations, some alleles may be lost just by chance, because the few
individuals that have them just happen not to reproduce. This can also happen
to relatively rare alleles in larger populations. This is called genetic drift.
Sickle cell anaemia and malaria Revised
Similarities between the global distribution of the genetic disease sickle cell
anaemia, and the infectious disease malaria, are a result of natural selection.
This illustrates the effect of environmental factors on allele frequencies.
l Sickle cell anaemia is caused by an allele of a gene coding for the
β polypeptide in haemoglobin. This is described on p. 47.
l Malaria is caused by a protoctist, Plasmodium, which is transmitted by
Anopheles mosquitoes. This is described on pp. 64–65.
A person who is homozygous for the faulty allele for the β polypeptide, HbSHbS,
has sickle cell anaemia. This is a serious disease and a child with this genotype is
unlikely to survive to adulthood and have children.
A person who is homozygous for the normal allele for the β polypeptide,
HbAHbA, is vulnerable to malaria. In parts of the world where the Anopheles
mosquitoes that transmit malaria are found, and where malaria is common, a
child with this genotype may not survive to adulthood and have children.
A person who is heterozygous for these alleles, HbAHbS, does not have sickle cell
anaemia. They are also much less likely to suffer from a serious attack of malaria
than a person with the genotype HbAHbA . Therefore a heterozygous person is
the most likely to survive to adulthood and have children.
The allele HbS therefore continues to survive in populations where malaria is
present, because it is passed on from generation to generation by heterozygous

people. In parts of the world where malaria is not common, there is no selective

advantage in having this allele, and it is very rare (Figure 17.2).
Distribution of malaria
Sickle cell anaemia high (>10% population)

Sickle cell anaemia significant (>2%)
Figure 17.2 The global distribution of malaria and sickle cell anaemia
The Hardy–Weinberg principle Revised
The Hardy–Weinberg principle states that, as long as certain conditions are

fulfilled, the frequency of a particular allele is likely to remain approximately the
same over many generations. These conditions are as follows:
l The population is large.
l There is random mating between individuals in the population.
l There are no significant selection pressures that give particular genotypes an
advantage or disadvantage.
l There are no new mutations.
l There is no introduction of new alleles by immigration.
If all of these conditions are fulfilled, we can use the Hardy–Weinberg equations
to calculate the frequencies of two alleles of a gene, for example A and a, in a
population.
Equation 1
p+q=1
where p is the frequency of allele A in the population and q is the frequency of
allele a.
Frequencies are expressed as decimals. For example, if there are equal numbers
of each allele in the population, then p and q are both 0.5.
Equation 2
p2 + q2 + 2pq = 1
This equation represents the frequencies of the different genotypes in the
population:
l p2 is the frequency of individuals with genotype AA
l q2 is the frequency of individuals with genotype aa
l 2pq is the frequency of individuals with genotype Aa

Using the Hardy–Weinberg equations
These equations can be used to calculate the frequencies of the alleles in a
population. For example, let us say that 2 in every 100 people in a population
have a genetic conditions that we know is caused by a recessive allele, a. This
frequency can be written as 0.02. These people must all be homozygous for this
recessive allele, so they are represented by q2. So:
q2 = 0.02
So:
q = √0.02 = 0.14
p+q=1
So:
Now test yourself
p = 1 − 0.14 = 0.86
2 In a population of 5000 animals,
We can therefore say that the frequency of the recessive allele a in the 100 have a genetic condition
population is 0.14 (which is the same as 14%) and the frequency of the dominant caused by a recessive allele.
allele A in the population is 0.86 (86%). Calculate:
a the frequency of the recessive
We can also calculate the frequency of carriers in the population. Their
allele in the population
frequency is 2pq, which is:
b the frequency of heterozygous
2 × 0.14 × 0.86 = 0.24 animals in the population
We would therefore expect just under one quarter of individuals in the Answers on p.206
population to be carriers of the recessive allele a. Tested
Selective breeding Revised
Selective breeding is the choice, by humans, of which animals or plants are

allowed to breed. This process is sometimes known as artificial selection.
Breeding cattle with high milk yields

Dairy cattle are breeds of cattle that are kept for milk production. Only females
(cows) produce milk. Selective breeding is therefore done by using cows that
have high milk yields and bulls whose female relatives (mothers, sisters) have high
milk yields.
If a particular bull is proved to have many female offspring with high milk yields,
then semen can be taken from him and used to inseminate many cows, using
artificial insemination procedures.
Selective breeding has produced massive increases in the volume and quality of
milk produced by cows (Figure 17.3).
Milk yield 9000

per cow/
kg 8000
7000
6000
5000
4000
1957 1962 1967 1972 1977 1982 1987 1992 1997 2002
Year
Figure 17.3 Mean milk yield per cow in Sweden between 1957 and 2002

However, such intensive selective breeding can inadvertently cause health

problems for the cattle. For example, producing so much milk can weaken their
general health. Carrying such large quantities of milk in their udders (mammary
glands) can cause problems with leg joints.
Breeding disease-resistant varieties of wheat and rice

Farmers may want to have wheat or rice plants that produce large yields of grain
and that are resistant to fungal disease such as rust. They want the plants to
be short, so that more energy can be put into growing grain (seed) rather than
wasted on stems, and also so that the plants are less likely to fall over in heavy
rain or high wind.
Breeders can choose a wheat or rice plant that is short, with high yields of grain,
and another that does not have these characteristics but is very resistant to rust.
They take pollen from one plant and place it on the stigmas of the other. (The
anthers of the second plant are first removed, so that it cannot pollinate itself.)
The resulting seeds are collected and sown. The young plants are allowed to
grow to adulthood. Then the plants that show the best combination of desired
characteristics are bred together. This continues for several generations, until the
breeder has a population of plants that have high yield and high resistance to rust.
Breeding dwarf varieties of crop plants

We have seen that gibberellin causes plant stems to grow longer. Some varieties
of crop plants have mutant alleles of the gibberellin gene, and do not produce as
much gibberellin. These dwarf plants are often higher yielding than tall varieties,
because they do not ‘waste’ energy in growing tall. They may also be more able
to stand upright in strong winds or heavy rain. Selective breeding can be used to
combine this characteristic with high yield.
Inbreeding and hybridisation in maize

Farmers require crop plant varieties in which all the individuals are genetically
identical. This means that, if the seed is sown at the same time and in the same
conditions, then the plants will all grow uniformly. They will ripen at the same
time and grow to the same height, which makes harvesting easier. The grain will
all have similar characteristics, which makes marketing easier.
Genetic uniformity is usually achieved through inbreeding (breeding a plant
with itself, or with other plants with the same genotype) for many generations.
However, in maize, inbreeding results in weak plants with low yields. This is
called inbreeding depression.
Maize breeding therefore involves producing hybrids between two inbred
lines. Like most selective breeding of cereal crops, it is done by commercial
organisations, not by farmers themselves.
l Inbred lines (genetically uniform populations) of maize with desirable
characteristics are identified, and crossed with other inbred lines with
different sets of desirable characteristics.
l The resulting hybrids are assessed for features such as yield, ability to grow in
dry conditions, and resistance to insect pests. The best of these hybrids are
then chosen for commercial production.
l Large quantities of the two inbred lines from which the hybrid was bred are
grown, as it is from these that all the seed to be sold will be produced.
l Each year, the two inbred lines are bred together, and the seed collected
from them to sell as hybrid seed.
This method of breeding avoids problems of inbreeding depression. The hybrid
plants are genetically uniform (although they will be heterozygous for several
genes) because they all have the same two inbred parents.

Notice:
l Artificial selection is similar to natural selection, in that individuals with

particular characteristics are more likely to breed than others.
l However, in artificial selection, individuals without these characteristics will
not breed at all, whereas in natural selection there is still a chance that they
might breed.
l Artificial selection may therefore produce bigger changes in fewer
generations than natural selection normally does.
l The breeders do not need to know anything about the genes or alleles that
confer the characteristics they want their plants to have; they just choose
the plants with those characteristics and hope that the appropriate alleles
will be passed on to the next generation.
l Artificial selection usually requires many generations of selection before the
desired result is obtained.
Evolution Evolution is the change in the

characteristics of a population or a
The theory of evolution states that species have changed over time. One species species over time.
can, over time, give rise to one or more new species.
Molecular evidence for evolutionary relationships Revised
There is a large quantity of evidence to support the theory of evolution. One

example of this evidence is the comparison of base sequences in DNA, or amino
acid sequences in proteins, in different organisms.
Mitochondria contain a single DNA molecule that is passed on down the female
line. Analysis of mitochondrial DNA (mtDNA) can be used to determine how
closely related two different species are. The more similar the sequences of bases
in their DNA, the more closely related they are considered to be.
Amino acid sequences in proteins can be used in a similar way. For example, the
protein cytochrome c, involved in the electron transport chain, is found in a very
wide range of different organisms, suggesting that that they all evolved from a
common ancestor. Differences in the amino acid sequences in cytochrome c
suggest how closely or distantly related particular species are.
Speciation Revised
A species is often defined as a group of organisms with similar morphological

Speciation is the production of one
and physiological characteristics, which are able to breed with each other to
or more new species from an existing
produce fertile offspring. So, for example, lions and tigers are distinct species,
species.
even though in a zoo they may be persuaded to breed together. Such
interbreeding between the two species never occurs in the wild and, in any case,
the offspring are not able to breed themselves.
So how are new species produced? We have seen that natural selection can
produce changes in allele frequency in a species, but how much change is
needed before we can say that a new species has been formed?
The crucial event that must occur is that one population must become unable
to interbreed with another. They must become reproductively isolated from
one another. Once this has happened, we can say that the two populations are
now different species.

Allopatric speciation
A group of individuals in the population may become geographically
separated from the rest. If speciation occurs as a result of geographical
separation, it is known as allopatric speciation.
For example, a few lizards might get carried out to sea on a floating log, and be
carried to an island where that species of lizard was not previously found. The
lizards on this island are subjected to different environmental conditions from
the rest of the species left behind on the mainland. Different alleles are therefore
selected for in the two groups. Over time, the allele frequency in the lizards
on the island becomes very different from the allele frequency in the original,
mainland lizards. This may cause their characteristics to become so different that
— even if a bridge appears between the island and the mainland — they can no
longer interbreed to produce fertile offspring.
Sympatric speciation
Two groups of individuals living in the same area may become unable to breed
together — for example, because one group develops courtship behaviours that
no longer match the courtship behaviour of the other group, or because they
live in different habitats in the same area (ecological separation). This is known as
sympatric speciation.
Isolating mechanisms
Possible reasons for the failure of two groups to interbreed include the following:
l They have evolved different courtship behaviours, so that mating no longer
occurs between them.
l The sperm of one group are no longer able to survive in the bodies of the
females of the other group, so fertilisation does not occur.
l The number or structure of the chromosomes is different, so that the zygote
that is formed by fertilisation does not have a complete double set of genes
and cannot develop.
l Even if a zygote is successfully produced, the resulting offspring may not be
able to form gametes, because its two sets of chromosomes (one from each Now test yourself
parent) are unable to pair up with each other successfully and so cannot
3 Which of the listed isolating
complete meiosis. mechanisms are pre-zygotic, and
These isolating mechanisms can be divided into pre-zygotic and post-zygotic which are post-zygotic?
mechanisms. Pre-zygotic mechanisms act before a zygote is formed. Post-zygotic Answers on p.206
mechanisms act after a zygote is formed. Tested
Extinction
The fossil record shows us that many species of organism used to live on Earth
but now no longer exist. There are been several times when very large numbers
of species became extinct, and these are known as mass extinctions. For
example, all dinosaur species disappeared from the Earth about 66 million years
ago. There is still uncertainty about exactly why this happened, but it is thought
to have resulted from severe climate change following a collision of a large
asteroid with Earth and/or very extensive volcanic eruptions.
In general, extinctions tend to be caused by:
l climate change; for example, global warming can result in some species not
being able to find habitats to which they are adapted for survival
l competition; for example, a newly evolved species, or one that migrates
into a new area, may out-compete a resident species, which then becomes
extinct
l habitat loss; for example, if humans cut down large areas of rainforest
l direct killing by humans

18 Biodiversity, classification
and conservation
Biodiversity Biodiversity is the variety of

ecosystems, habitats, species and
Biodiversity includes: genotypes that exists in an area.
l the range of ecosystems or habitats Species (see p. 160).
l the numbers of different species Ecosystem is an interacting system of
l the genetic variation that exists within the populations of each species in a all the living and non-living things in a
particular area defined area.
Niche is the role of a species in an
You need to know definitions of species, ecosystem and niche. ecosystem.
Determining the biodiversity of an area Revised
We can find out something about the biodiversity of an area by measuring the
species richness. This is the number of different species in the area. The greater
the species richness, the greater the biodiversity.
Assessments of biodiversity often involve measuring the distribution and
abundance of the different species. You cannot usually count every single
organism in the area, so a sampling technique is used. It is important that this is
random, to avoid bias in your choice of area in which to make your Typical mistake
measurements. For example, you could use a mobile phone app to generate
The correct spelling is quadrat, not
random numbers. These numbers can be used as x and y coordinates to tell you quadrant.
where to place a quadrat within a defined area.
Methods of sampling
Frame quadrats
This is a square frame that is placed onto the ground to define an area within
which organisms are counted. Within the quadrat, you can either count the
actual numbers of organisms (e.g. limpets on a rocky sea shore) or estimate the
percentage cover of each species (e.g. different species of plants in meadow).
Line transects
Frame quadrats are often placed randomly (see above) in the area to be studied.
However, sometimes you want to know how species abundance changes along
a particular line — for example, from the bottom of a beach to the top, or from
a heavily shaded area to a sunnier part of a meadow. To do this, you can place
quadrats along a transect. Lie a long measuring tape on the ground, and then
place your quadrats at equal intervals (say, every 2 metres) along the tape.
Belt transects
You can also sample the species at every point along the tape. This can be done
by placing your quadrats in succession along the tape, with no gaps between
them; or by recording every organism that is touching the tape itself.
Mark–release–recapture
This is a method for estimating the population of species of mobile animal.
l First, a large number of animals is captured. Each one is marked in a way that
will not affect its chances of survival. For example, a mouse could have small
patch of fur cut away; a woodlouse could have a small spot of paint applied.
The number of marked animals is recorded.

l The marked animals are released, and time is allowed for them to spread

naturally back among the population.
Now test yourself
l A second sample is captured. The number of animals is recorded, and also 1 A student caught 38 woodlice and
the number of these that were marked. marked them. He released them
back into the place from where
The total size of the population can then be estimated by this calculation: they were caught. Two days later,
number in first sample × number in second sample he caught 40 woodlice in the same
number in population = area. Six of them were marked.
number of marked animals in second sample
Calculate the estimated size of the
woodlouse population.
Answers on p.206
Tested
Analysing your results Revised
Spearman’s rank correlation

This is a statistical test that can be used to see if there is a correlation between
the distribution and abundance of two species, or if there is a correlation
between the distribution and abundance of a species, and a particular
environmental factor. This test is described on pp. 188–189.
Pearson’s linear correlation

This test tells you if there is a linear correlation between the distribution
and abundance of two species, or if there is a linear correlation between the
distribution and abundance of a species, and a particular environmental factor.
This test is described on p. 190.
Simpson’s index of diversity (D)

This is calculated using the formula:
D = 1 − ∑
n2
N ()
where:
D = Simpson’s index of diversity
n = the total number of individuals of one species
N = the total number of all individuals of all species in your sample
The greater the value of D, the greater the biodiversity.
2 Use Simpson’s index of diversity to determine the species diversity of an area from which these results were obtained.
Species Number of individuals, n

A 2
B 35
C 1
D 81
E 63
F 2
G 5
H 11
I 1
total number of individuals = 201
Answer on p.206
Biodiversity, classification and conservation 163

Classification
Biologists classify organisms according to how closely they believe they are
related to one another. Each species has evolved from a previously existing
species. We do not usually have any information about these ancestral species,
so we judge the degree of relatedness between two organisms by looking
carefully at their physiology, anatomy and biochemistry. The greater the
similarities, the more closely they are thought to be related.
The system used for classification is a taxonomic system. This involves placing
organisms in a series of taxonomic units that form a hierarchy. The largest unit
is the domain. There are three domains: Archaea, Bacteria and Eukarya. The
Archaea and Bacteria are prokaryotic (see p. 12 for the features of prokaryotic
cells), but are now known to differ greatly in their metabolism. The Eukarya are
eukaryotic organisms, many of which are multicellular.
The taxonomic hierarchy Revised
The Eukarya are divided into four kingdoms.

Kingdom Protoctista These organisms have eukaryotic cells. They mostly exist
as single cells, but some are made of groups of similar cells.
Kingdom Fungi Fungi have eukaryotic cells surrounded by a cell wall, but this is
not made of cellulose and fungi never have chloroplasts.
Kingdom Plantae These are the plants. They have eukaryotic cells surrounded
by cellulose cell walls and they feed by photosynthesis.
Kingdom Animalia These are the animals. They have eukaryotic cells with no
cell wall.
Kingdoms are subdivided into phyla, classes, orders, families, genera and
species (Figure 18.1).
Kingdom Animalia
Phylum Chordata
Class Mammalia
Order Rodentia
Family Muridae
Genus Mus
Species Mus musculus (house mouse)
Figure 18.1 An example of classification
Note that viruses are not included in the three-domain classification. This
is because they are on the borderline between living and non-living things.
They have none of the characteristics of living things, except for the ability to
reproduce. However, even this can only be done when they have entered a host
cell, where they use the host cells’ ability to copy, transcribe and translate base
sequences, in order to synthesis proteins and nucleic acids to make new viruses.
See p. 13 for a diagram of a virus.
Viruses are classified according to the type of nucleic acid that they contain —
that is, whether it is DNA or RNA. They can be further classified according to
whether the nucleic acid is single-stranded or double-stranded.

Conservation

Conservation is the maintenance or
increase of biodiversity in an area.
Conservation aims to maintain biodiversity.
Reasons for maintaining biodiversity Revised

l Stability of ecosystems The loss of one of more species within a
community may have negative effects on others, so that eventually an entire
ecosystem becomes seriously depleted.
l Benefits to humans The loss of a species could prevent an ecosystem from
being able to supply humans with their needs.
l For example, an ancient civilisation in Peru, the Nazca people,
are thought to have cut down so many huarango trees that their
environment became too dry and barren for them to grow crops.
l Today we make use of many different plants to supply us with drugs.
There may be many more plant species, for example in rainforests, that
could potentially provide life-saving medicines.
l In some countries, such as Kenya, people derive income from tourists
who visit the country to see wildlife.
l Several scientific studies have found that people are happier and healthier
if they live in an environment with high biodiversity.
l Moral and ethical reasons Many people feel that it is clearly wrong to
cause extinction of a species, and that we have a responsibility to maintain
environments in which all the different species on Earth can live.
Threats to biodiversity Revised
We have seen (p. 161) that there have been mass extinction events in the past,
when huge numbers of species became extinct over a relatively short period of
time. We are currently experiencing another potential mass extinction event.
Much of this is due to human activity. For example:
l Increases in greenhouse gases (mostly carbon dioxide and methane) are
causing global warming. Much of this increase is caused by human activities,
such as burning fossil fuels. Global warming is changing the environmental
conditions in many habitats, including raised temperatures, changes in rainfall
patterns and a higher frequency of extreme events such as hurricanes. Some
species may no longer be able to find suitable habitats in which to live, and
will become extinct.
l As the human population continues to increase, we make ever-increasing
demands on the environment for resources. Land that could support natural
ecosystems and high biodiversity is taken over by humans for farming,
building towns, mining and building roads.
l Pollutants — for example, heavy metals, acidic gases (such as SO2, NOx)
and phosphates (from detergents) — released into the air, water and soil
may change the environment in ways that reduce the chance of survival of
organisms, and could make species extinct.
l Introduction of alien species can threaten native species. For example, the
introduction of possums into New Zealand has threatened many native
birds with extinction. Possums eat large quantities of leaves from native trees,
leaving less food for native species and less diversity of trees in forests.
Also see below for the particular reasons for the threat to tigers.

Endangered species
A species is said to be endangered if its numbers have fallen so low that it may
not be able to maintain its population for much longer. For example, tigers are
seriously endangered. This has happened for many reasons:
l Tigers are hunted by humans for various body parts that are thought to be
effective medicines by people in countries such as China and Korea.
l Tigers require large areas of land to live and hunt successfully. Expanding
human populations also require land, and this has greatly reduced the area of
suitable habitat for tigers.
l Tigers are large predators that can pose threats to humans and their
livestock, and so may be killed.
It is generally considered desirable for a species to have reasonably large genetic
diversity. This means that if the environment changes — for example, because
of climate change or if a new pathogen emerges — then at least some of the
population may possess features that will enable them to survive. Genetic
diversity allows species to become adapted to a changing environment.
The tiger populations in many areas are now so low that genetic diversity is
small. This makes it more likely that an individual tiger will inherit the same
recessive allele from both parents, which may produce harmful characteristics. It
also makes it less likely that the population will be able to adapt (through natural
selection acting on natural variation) to changes in its environment.
Methods of protecting endangered species

Habitat conservation
The best way to conserve threatened species is in their own natural habitat.
For example, national parks, marine parks and nature reserves can set aside
areas of land or sea in which species are protected. A species will only survive
if the features of its habitat that are essential to its way of life are maintained.
Maintaining habitat is also likely to be beneficial to many other species in the
community.
However, this is often difficult because people living in that area have their own
needs. In parts of the world where people are already struggling to survive, it
is difficult to impose restrictions on the way they can use the land where they
live. The best conservation programmes involve the local people in habitat
conservation and reward them for it in some way. For example, they could be
given employment in a national park, or could be paid for keeping a forest in
good condition. Expert tip
A habitat that has been severely damaged is said to be degraded. It is Try to find out about a local example
sometimes possible to restore degraded habitats. For example, a heavily polluted of a degraded habitat and how it is
river, or one that has been over-engineered (i.e. its channel has been being restored, so that you could
give details of this in an answer to an
straightened, or its banks have been concreted over) can be restored to a more examination question.
natural state.

Zoos
Captive breeding programmes This involves collecting together a small
group of organisms of a threatened species and encouraging them to breed
together. In this way, extinction can be prevented. The breeding programme
will try to maintain or even increase genetic diversity in the population by
breeding unrelated animals together. This can be done by moving males from
one zoo to another, or by using in vitro fertilisation (see below) with frozen
sperm transported from males in another zoo. It may also be possible to implant
embryos into a surrogate mother of a different species, so that many young can
be produced even if there is only a small number of females of the endangered
species. Eggs and embryos, like sperm, can be frozen and stored for long periods
of time. Collections of frozen sperm, eggs or embryos are sometimes known as
‘frozen zoos’.
Reintroduction programmes The best captive breeding programmes work
towards reintroducing individuals to their original habitat, if this can be made
safe for them. It is very important that work is done on the ground to prepare
the habitat for the eventual reintroduction of the animals. For example, the
scimitar-horned oryx has been successfully reintroduced to Tunisia, following a
widespread captive breeding programme in European zoos and the preparation
and protection of suitable habitat, including the education and involvement of
people living in or around the proposed reintroduction area.
Education Zoos can bring conservation issues to the attention of large numbers
of people, who may decide to contribute financially towards conservation efforts
or to campaign for them. Entrance fees and donations can be used to fund
conservation programmes both in the zoo itself and in natural habitats.
Research Animals in zoos can be studied to find out more about their needs
in terms of food, breeding places and so on. This can help to inform people
working on conservation in natural habitats.
Botanic gardens
Botanic gardens are similar to zoos, but for plants rather than animals. Like
zoos, they can be safe havens for threatened plant species, and are involved in
breeding programmes, reintroduction programmes, education and research.
Seed banks
Seed banks store seeds collected from plants. Many seeds will live for a very long
time in dry conditions, but others need more specialised storage environments.
A few of the seeds are germinated every so often so that fresh seed can be
collected and stored.
Seed banks can help conservation of plants just as zoos can help conservation of
animals. The Royal Botanic Gardens at Kew in the UK has a huge seed bank at
Wakehurst Place, Sussex, UK. Collectors search for seeds, especially those of rare
or threatened species, and bring them to the seed bank where they are carefully
stored. Another seed bank, built into the permafrost (permanently frozen
ground) in Norway, aims to preserve seeds from all the world’s food crops.
Methods of assisted reproduction

It is often desirable to speed up the natural reproduction rate of animals in zoos,
in order to increase the number of individuals of the species more rapidly.
IVF
This stands for in vitro fertilisation. ‘In vitro’ means ‘in glass’. This method may
be necessary if animals do not reproduce in captivity, perhaps because the
conditions are not entirely suitable to stimulate them to do so. It can also enable
genetic diversity to be maintained, by transferring frozen sperm from an animal
in one zoo, to be used to fertilise eggs from an unrelated female in another zoo.

Eggs are taken from a female. She may be given hormones before this is done, to Typical mistake
make her produce more eggs than usual. Sperm is taken from a male. The eggs IVF is often confused with artificial
and sperm are placed in a sterile container, in a suitable liquid, where the sperm insemination, AI. AI involves artificially
will fuse with the eggs and fertilise them. The zygotes that are formed are then introducing sperm (in semen) to a
inserted into the uterus of the female. female’s reproductive system.
Embryo transfer and surrogacy

IVF may produce more zygotes than can be implanted in one female. Embryos
formed from one female’s eggs can be inserted into the uteruses of other
females, which are known as surrogate mothers. In some cases, the embryos
may be put into the uteruses of other, closely related, non-endangered species.
Preventing overpopulation Revised
In many seemingly natural ecosystems, human activities have removed top

predators, such as wolves. This can allow the populations of their prey species,
such as deer, to become very numerous. This in turn may threaten other
organisms in the ecosystem; for example, herbivores can overgraze the available
vegetation, which can result in soil erosion and/or the extinction of plant species.
Conservation may therefore require human intervention to control the
populations of a species, even when this species is protected. This can be
done by:
l culling, where selected animals in a population are killed. This may be done
by hunters, who are given licences allowing them to kill a certain number
of animals of a particular sex — for example, deer culling in the USA.
Sometimes, culling is done in zoos, where a particular animal is deemed not
to be suitable for breeding.
l contraception. For example, in South Africa, female elephants may be given
vaccines that cause them to produce antibodies that prevent sperm from
fertilising their eggs.
Roles of NGOs Revised
NGO stands for non-governmental organisation. These are organisations that

are not funded by a country’s government, but by charitable donations. There
are many international NGOs, and most countries also have more local NGOs
working actively in conservation. One of the most important international
conservation NGOs is WWF, the Worldwide Fund for Nature. It receives
donations from individuals and organisations all over the world, and uses
them in programmes that include attempts to conserve individual species (for
example, snow leopards), to train local people to become wildlife wardens, or to
plant native trees to re-establish forests.
International treaties Revised
Although some conservation can be managed locally, some issues require

international cooperation. For example, governments of many countries have
signed up to an agreement called CITES, the Convention on International Trade
in Endangered Species. This treaty sets out rules that prevent listed species,
or products derived from them (such as rhino horn), being moved from one
country to another.

Genetic technology, or genetic engineering, is the manipulation of genes in living

Recombinant DNA is DNA with base
organisms. Genes extracted from one organism can be inserted into another.
sequences that would not normally be
This can be done within the same species (for example, in gene therapy) or present in an organism; this is usually
genes may be transferred from one species to another. Genes can also be the result of the introduction of DNA
synthesised in the laboratory by linking DNA nucleotides together in a particular from a different species.
order, and then inserting this gene into an organism. The transferred gene is then
expressed in the recipient organism.
These processes produce recombinant DNA — that is, DNA whose base Now test yourself
sequences would not normally be found in an organism. 1 How does gene technology differ
from selective breeding?
Answers on p.206
Tools for genetic Tested
technology
PCR Revised
PCR stands for polymerase chain reaction. It is an automated method of

making multiple, identical copies (cloning) of a tiny sample of DNA.
PCR involves the exposure of the DNA to a repeating sequence of different
temperatures, allowing different enzymes to work. The process shown in
Figure 19.1 is repeated over and over again, eventually making a very large
number of identical copies of the original DNA molecule.
The primer is a short length of DNA with a base sequence complementary
to the start of the DNA strand to be copied. This is needed to make the DNA
polymerase begin to link nucleotides together as it makes a copy of the exposed
DNA strand.
The DNA polymerase that is used in PCR is called Taq polymerase. This enzyme
was sourced from a bacterium called Thermophilus aquaticus, which lives in
hot springs in Yellowstone Park in the USA. Its enzymes are stable even at high
temperatures, which is why it is suitable for catalysing DNA synthesis in PCR.
Gel electrophoresis Revised
Electrophoresis is a way of separating:

l strands of DNA of different lengths, or
l polypeptides with different base sequences
It does this by applying an electrical potential difference across a gel in which a

sample is placed. Lengths of DNA or polypeptides of different masses, or with
different charges, will move across the gel at different speeds.
Genetic technology 169

DNA strand 1 DNA is extracted.
3´ 5´
5´ 3´
Gene to be copied
3´ 5´ 2 The DNA is heated to 95 ºC to

denature it, which separates the
double helix.
5´ 3´
3 The DNA is cooled to 65 ºC, and

Primer primer DNA is added.
Complementary base pairing occurs.
4 The DNA and primers are incubated

at 72 ºC with DNA polymerase and
free nucleotides to synthesise
complementary strands of DNA.
5 The DNA has been copied, and each

of the two new strands form part
of two DNA molecules.
6 The DNA is heated to 95 ºC again to

denature the DNA, and a new cycle of
copying occurs, following steps 2–5.
This is repeated many times to

synthesise many new copies of DNA.
Figure 19.1 The polymerase chain reaction

Before electrophoresis of DNA is carried out, the sample of DNA is exposed to
a set of restriction enzymes (restriction endonucleases). These enzymes
cut DNA molecules where particular base sequences are present. For example,
a restriction enzyme called BamH1 cuts where the base sequence -GGATCC- is
present on one strand of the DNA. Other restriction enzymes target different
base sequences. This cuts the DNA into fragments of different lengths.
To carry out gel electrophoresis, a small, shallow tank is partly filled with a layer
of agarose gel. A potential difference is applied across the gel, so that a direct
current flows through it.
A mix of the DNA fragments to be separated is placed on the gel. DNA
fragments carry a small negative charge, so they slowly move towards the
positive terminal. The larger they are, the more slowly they move. After some
time, the current is switched off and the DNA fragments stop moving through
the gel.
The DNA fragments must be made visible in some way, so that their final
positions can be determined. This can be done by adding fluorescent markers

to the fragments. Alternatively, single strands of DNA made using radioactive
isotopes, and with base sequences thought to be similar to those in the DNA
fragments, can be added to the gel. These are called probes. They will pair up
with fragments that have complementary base sequences, so their positions are
now emitting radiation. This can be detected by its effects on a photographic
plate (Figure 19.2).
DNA
Two different restriction enzymes
cut the DNA into fragments.
DNA Fragments are selected

and multiplied.
DNA fragments are A radioactive probe

put into a gel and is added to bind to
separated by an the invisible bands
electric field. of DNA, so they
can blacken an
X-ray film.
− +
Figure 19.2 Gel electrophoresis
Applications of gel electrophoresis

Distinguishing between polypeptides or proteins
Polypeptides are made up of long chains of amino acids. These can differ in the
charge they carry, because different R groups (p. 20) have different charges. For
example, we have seen that sickle cell haemoglobin has an amino acid with an
uncharged R group in the β polypeptide, whereas normal haemoglobin has an
amino acid with a charged R group. This difference in charge means that the
two different β globins will move at different speeds on the electrophoresis gel.
Distinguishing between different alleles of a gene

DNA strands containing different alleles of a gene may end up at different places
on the gel after electrophoresis. For example, one allele may have more bases
than another, and so be more massive and move more slowly.
Genetic fingerprinting
Some regions of human DNA are very variable, containing different numbers of
repeated DNA sequences. These are known as variable number tandem repeats,
or VNTRs. Each person’s set of VNTR sequences is unique. Only identical twins
share identical VNTR sequences.
Restriction enzymes are used to cut a DNA sample near VNTR regions. The
chopped pieces of DNA are then separated using gel electrophoresis. Long
VNTR sequences do not travel as far on the gel as short ones. The pattern
of stripes produced is therefore determined by the particular combination of
VNTRs that a person has.
Genetic fingerprinting can be used to determine whether:
l a sample of semen, blood or other tissue found at a crime scene could have
come from the victim or a suspect
l a particular person could be the child, mother or father of another
(Figure 19.3).

The dark areas on these
autoradiographs of DNA
represent particular
DNA sequences.
However, on the
In the first profile, you second one, the child
can see that all the has a band that is
bands on the child’s not present in either
results match up with the mother’s or
either the mother’s or father’s results. Some
potential father’s, so other person must
this man could be the therefore be the
child’s father. Mother
child’s father.
Child
Alleged
father
Mother
Child
Alleged
father
Figure 19.3 Electrophoresis in paternity testing
Using bacteria to make human insulin Revised
In order to understand how genetic technology can produce recombinant

organisms, we will look at the production of recombinant DNA containing the
human insulin gene and its insertion into bacteria, which then express the gene
and make insulin.
Insulin is a small protein. It is a hormone secreted by β cells in the islets of
Langerhans in the pancreas in response to raised blood glucose concentration
(p. 120).
In type I diabetes, no or insufficient insulin is secreted, and the person has to
inject insulin. This used to be obtained from animals such as pigs. Today, almost
all insulin used in this way is obtained from genetically modified bacteria.
Identifying the insulin gene Protein Val Asn Gln His Leu Cys
The amino acid sequence of insulin was already known. From this, the probable
G T CAA T CAGCACC T T T G T
base sequence of the gene that codes for it, and of the mRNA transcribed from DNA CAG T T AG T CG T GGAAACA
the gene, could be worked out (Figure 19.4).
RNA CAGUUAGUCGUGGAAACA
Making the human insulin gene Figure 19.4

Messenger RNA was extracted from β cells. These cells express the gene
for insulin, so much of this mRNA had been transcribed from this gene.
The appropriate mRNA was then incubated with the enzyme reverse mRNA
transcriptase, which built single-stranded cDNA molecules against it. These
were then converted to double-stranded DNA — the insulin gene (Figure 19.5).
mRNA
Some extra single-stranded DNA was then added to each end of the DNA
molecules. These are called sticky ends. Because they are single-stranded, they
cDNA
are able to form hydrogen bonds with other single-stranded DNA, enabling
DNA molecules to join up with one another. This is important in a later stage of
the process. Double-
stranded
Cloning the DNA DNA
Multiple copies of the DNA were then made (Figure 19.6) using PCR
(p. 169). Figure 19.5
Figure 19.6

Inserting the DNA into a plasmid vector
A plasmid is a small, circular DNA molecule found in many bacteria. A plasmid
was cut open using a restriction endonuclease. The restriction endonucleases
made a stepped cut across the DNA molecule, leaving single-stranded regions
(Figure 19.7).
Figure 19.7
The cut plasmids and the insulin gene (the cloned DNA) were then mixed
together, along with the enzyme DNA ligase. Complementary base pairing took Insulin gene
place between the sticky ends added to the insulin genes and the sticky ends of
the cut plasmids. DNA ligase then joined up the sugar–phosphate backbones
of the DNA strands. This resulted in closed plasmids containing the insulin gene
(Figure 19.8).
Genes conferring resistance to an antibiotic were also introduced into the Figure 19.8
plasmids, next to the insulin gene.
Not all of the plasmids took up the gene. Some just closed back up again
without it.
Inserting the plasmid into a bacterium E. coli
The plasmids were mixed with a culture of the bacterium Escherichia coli.
About 1% of them took up the plasmids containing the insulin gene Figure 19.9
(Figure 19.9).
Identifying the genetically modified bacteria Bacteria
Antibiotics were then added to the culture of E. coli bacteria. The only ones that cultured
survived were the ones that had successfully taken up the plasmids containing on agar +
the antibiotic resistance gene. Most of these plasmids would also have contained antibiotic
the insulin gene. Most of the surviving E. coli bacteria were therefore ones that
now contained the human insulin gene (Figure 19.10). Surviving
bacteria
Cloning the bacteria and harvesting the insulin
The bacteria were then grown in fermenters, where they were provided with Figure 19.10
nutrients and oxygen to allow them to reproduce to form large populations
(Figure 19.11). Reproduction is asexual, so all the bacteria were genetically
identical (clones). Fermenter
These GM (genetically modified) bacteria are now cultured on a large scale.

The bacteria synthesise and secrete insulin, which is harvested from the
fermenters and purified before sale. Figure 19.11
Vectors Revised
In genetic technology, a vector is a structure that transfers the DNA into the
organism that is to be modified. Vectors can be:
l plasmids (see above), which are suitable for use as vectors because:
l they are small, so can easily be taken up by bacteria
l their small size makes it easy to manipulate them without having large
quantities of ‘extra’ DNA that are not required Revision activity

l they are made of a closed, circular molecule of DNA l Summarise the uses of these
l viruses, which are suitable for use as vectors because viruses naturally enzymes in gene technology: DNA
enter cells ligase, restriction endonuclease,
DNA polymerase, reverse
l liposomes, which are small balls of lipid containing protein; being lipid, they transcriptase.
are able to move easily through cell surface membranes and enter cells

Promoters
Revised
In bacteria, each gene is associated with a region of DNA called a promoter

(p. 153). The enzyme RNA polymerase must bind to the promoter before it
can begin transcribing the DNA to produce mRNA.
It was therefore important to ensure that there was a promoter associated with
the human insulin gene when it was inserted into E. coli.
Markers Revised
The antibiotic resistance genes added to the plasmids along with the human
insulin gene act as markers. They make it possible to identify the bacteria that
have taken up the gene.
There is concern that using antibiotic resistance genes as markers could increase
the likelihood of the development of populations of harmful bacteria that are
resistant to antibiotics. Today, the most common markers used are genes that
code for the production of fluorescent green protein. The gene for this
protein can be inserted along with the desired gene. Cells that fluoresce green
are therefore likely to have taken up the desired gene.
Microarrays Revised
A microarray is a tool used to identify particular DNA sequences in a sample

of DNA. The microarray is a small piece of glass (often 2 cm2) to which DNA
probes (see p. 171) have been attached in a regular pattern. As many as 10 000
different probes can be attached to one microarray.
The DNA to be tested is cut into fragments using restriction endonucleases,
and is then exposed to the microarray. Fragments whose base sequences are
complementary to one of the probes will attach to it by complementary base
pairing. The microarray can then be ‘read’ by a computer, which will identify
the probes to which DNA fragments have attached, and therefore the base
sequences present in the DNA sample.
This technique can be used to compare the genome (all the DNA present) in
two different species, or in two individuals of the same species.
The technique can also be used to identify which genes are being expressed
in a cell. mRNA is extracted from the cell, and then used to make cDNA (see
p. 172). The cDNA is then analysed using a microarray. Any DNA that attaches
to the probes must represent a gene that was being expressed and producing
mRNA in the cell.
Genetic technology
applied to medicine
Bioinformatics Revised
Bioinformatics is the use of large databases and computer software to store

and analyse information about living organisms. For example, there are now
databases listing the base sequences of all the DNA in several individual people,
as well as databases for the genomes of other species such as the nematode

worm Caenorhabditis and the malarial parasite Plasmodium. There are also
databases for the proteomes of different species. (The proteome is all the
different proteins in an organism.)
The information stored in these databases is available to scientists all over the
world. There are many potential uses for it. For example:
l People have different combinations of alleles of genes in their cells, and this
can affect their susceptibility to diseases and the way in which drugs work in
their bodies. It should one day be possibility to determine which is the best
drug to give to a person with a particular DNA profile.
l Knowing the detailed DNA sequences of different species or varieties of
Plasmodium can help scientists to develop drugs that will act against them.
l Matches can be made between DNA sequences and protein sequences,
which can help to unravel how genes affect metabolism and therefore
health.
l Models can be made of enzymes using amino acid sequences derived from
the proteome, and drugs can then be designed that could inhibit these
enzymes, using computer modelling.
Advantages of producing human proteins by gene technology Revised
Examples of human proteins produced by GM organisms include:

l insulin for the treatment of diabetes, produced by E. coli (see pp. 172–173)
l factor VIII for the treatment of haemophilia, produced by tissue cultures of
GM hamster cells
l adenosine deaminase for the treatment of severe combined
immunodeficiency (SCID), produced by GM bacteria
There are numerous advantages in using proteins produced in this way. For
example:
l The insulin produced by the genetically engineered E. coli is identical to
human insulin, because it is made by following the genetic code on the
human insulin gene. Insulin obtained from the pancreas of an animal is
slightly different, and therefore may have different effects when used to treat
diabetes in humans.
l Large quantities of insulin can be made continuously using E. coli, and this
can be done under controlled conditions. Only small quantities of insulin can
be obtained from the pancreas of an animal, and it is not easy to purify the
insulin to produce a standard product that is safe for medicinal use.
l Many religions and cultures, and also many individuals, are against the idea of
harvesting insulin from a dead animal for use in humans.
Genetic screening Revised
Finding out the genes that a person has is called genetic screening. This can be
used
l to identify people who are carriers, i.e. who have a copy of a harmful
recessive allele, such as the cystic fibrosis allele, the sickle cell anaemia allele or
the haemophilia allele; a couple with one of these genetic conditions in the
family could therefore find out if they are both heterozygous and therefore
might have a child with the condition
l in preimplantation genetic diagnosis, to check the genes of an embryo
produced in vitro (i.e. by fertilisation outside the body) before it is placed in
the mother’s uterus; this can ensure that only embryos that do not have the
genes for a genetic disease are implanted

l for prenatal testing, i.e. checking the genes of an embryo or fetus in the
uterus; this could enable the mother to decide to have her pregnancy
terminated if the baby would have a genetic disease
l to identify people who will develop a genetic condition later in life; for
example, Huntington’s disease is caused by a dominant allele, but does not
manifest itself until middle age; a person with this disease in the family could
check if they have the gene before they decide to have children themselves;
l to identify people with alleles that put them at risk of developing other
diseases; for example, a woman who has relatives with breast cancer could
find out if she has the BRCA1 or BRCA2 alleles, which are known to be
associated with an increased risk of breast cancer, and could decide to have a
mastectomy to prevent developing the illness
A genetic counsellor helps people to interpret the results of the screening
and therefore to make decisions. This can be very difficult, and involves moral
and ethical, as well as scientific, issues. For example, if a pregnant woman finds
that her child will have cystic fibrosis, are these sufficient grounds to have her
pregnancy terminated, or does the child have a right to life?
Treating genetic conditions using gene therapy Revised
Gene therapy is the treatment of a genetic disease by changing the genes in a

person’s cells. It is only suitable for:
l diseases caused by a single gene
l diseases caused by a recessive allele of a gene
l serious diseases for which treatment is limited and no other cure is possible
Although attempts have been made to treat several different diseases using gene
therapy, there are still many problems to be solved before treatments become
widely available and successful.
The vectors used for gene therapy can be liposomes or viruses. In a few cases,
trials have been carried out using ‘naked’ DNA.
Gene therapy for cystic fibrosis

Cystic fibrosis (CF) is a genetic condition resulting from a mutation in a
gene that codes for a transporter protein called CFTR. This protein lies in the
cell surface membrane of cells in many parts of the body, including the lungs,
pancreas and reproductive organs. The CFTR protein actively transports chloride
ions out of cells.
There are several different mutations that result in changes in the CFTR protein.
Some of them involve a change in just one base in the gene coding for the CFTR
protein. The commonest one, however, involves the loss of three bases from
the gene, meaning that one amino acid is missed out when the CFTR protein is
being made. In all cases, the protein that is made does not work properly.
Normally, chloride ions are transported out of the cells through the CFTR
protein. Water follows by osmosis. When the CFTR protein is not working, this
does not happen. There is therefore less water on the outer surface of the cells
than there should be. The mucus that is produced in these areas therefore does
not mix with water in the usual way. The mucus is thick and sticky. As a result:
l the abnormally thick mucus collects in the lungs, interfering with gas
exchange and increasing the chance of bacterial infections
l the pancreatic duct may also become blocked with sticky mucus, interfering
with digestion in the small intestine
l reproductive passages, such as the vas deferens, may become blocked,
making a person sterile

Attempts have been made to treat cystic fibrosis by introducing the normal
CFTR allele into a person’s cells. Two methods have been trialled:
l inserting the normal gene into a harmless virus and then allowing the virus
to infect cells in the person’s respiratory passages — the virus enters the cells
and introduces the gene to them
l inserting the gene into little balls of lipid and protein, called liposomes, and
spraying these as an aerosol into a person’s respiratory passages
The virus and the liposomes are said to be vectors — they transfer the gene
into the person’s cells.
In each case there was some success, in that some of the cells lining the
respiratory passages did take up the gene. Because the normal gene is dominant,
there only needs to be one copy in a cell for it to produce normal mucus. There
is no need to remove the faulty allele first, because it is recessive.
However, there were problems with the trials of gene therapy for cystic fibrosis:
l Only a few cells took up the normal gene, so only these cells produced
normal mucus.
l It was only possible for cells in the respiratory passages to take up the
normal gene, not cells in the pancreas or reproductive organs.
l Cells in the surfaces of the respiratory passages do not live for very long, so
treatment would need to be repeated every few weeks.
l When using the virus as a vector, some people developed serious lung
infections.
Gene therapy for other conditions

SCID
SCID is caused by a lack of the enzyme adenosine deaminase. This results from
mutations in the gene for this enzyme, which result in it not being expressed.
Trials have been carried out using viruses as vectors to transfer the normal
allele into white blood cells taken from a person with SCID. These trials have
been partly successful, with many patients now having a functional immune
system. However, there is no control over where the allele is inserted into the
patient’s DNA, and in some case this has resulted in genes controlling cell
division becoming altered and causing cancer (leukaemia). Different methods of
transferring the gene are now being trialled.
Choroideremia
This is a genetic condition in which cells in the retina gradually die, and is due
to a faulty allele of the CHM gene on the X chromosome. Several patients have
now had their eyesight partially restored after viruses carrying normal alleles of
the CHM gene were injected beneath their retinas in one eye.
Social and ethical considerations Revised
The ability to detect genetic conditions, and perhaps to cure some of them,
raises ethical questions that society needs to debate. You might like to think
about some of the questions raised by the examples given below, and consider
the answers to those questions. There are no ‘right’ or ‘wrong’ answers. Try to
think about two different viewpoints for each one.
l A person undergoes genetic screening and finds that they have a genetic
condition that cannot be treated. How does this affect their life?
l A couple undergo genetic screening and find that they both have a recessive
allele for an unpleasant but not life-threatening genetic condition. What do
they do?

l A mother finds that her embryo will have cystic fibrosis. Does she have her
pregnancy terminated? Now test yourself

l A person with a genetic condition that would have prevented them living to 2 Explain why gene therapy is
reproductive age has gene therapy, and then has children who have inherited most likely to succeed in treating
the faulty allele. What effects might this have on the population? conditions caused by a recessive
l A couple very much want a girl baby rather than a boy. They decide to have allele of a single gene.
IVF and have the sperm screened so that they can choose a sperm carrying Answers on p.206
an X chromosome to fertilise the egg. Should this be allowed? Tested
Genetically modified
organisms in agriculture
We have seen that crops can be improved by selective breeding. However, this
takes a long time, and can only involve alleles that are already present in the
genome of that crop species. Genetic engineering allows the targeted addition of
alleles for a desired characteristic into a species where that allele is not naturally
present. Although it is a highly technical and expensive process, it can achieve
results more quickly than conventional selective breeding.
Examples of GM crops and livestock Revised
Golden Rice
This is rice that has had genes encoding vitamin A added to it. In countries
where rice forms a major part of the diet, children can suffer from vitamin A
deficiency, causing blindness and severe deficiencies of the immune system,
which can result in death. Eating this GM rice provides more β carotene, which
is used to make vitamin A, and so can help to avoid this condition.
The company and researchers who developed the rice have donated it free
for use in developing countries. The GM rice has been interbred with locally
developed varieties of rice, so that it will grow well in different countries.
However, as yet no government has allowed it to be grown by farmers. Some
people argue that this is not the best way to solve problems associated with
poverty, and that it would be better to improve people’s lives so that they are
able to choose a wider variety of foods to eat. They are also concerned that the
GM rice might somehow be harmful to health, although there is no scientific
foundation for such concerns.
Insect-resistant maize, cotton and tobacco

Yields of maize can be greatly reduced by an insect larva called the corn borer.
Cotton yields are reduced by the cotton bollworm, which is also an insect larva.
Tobacco plant yields are affected by the tobacco budworm.
Pesticides sprayed onto the crops can kill these insects. However, the pesticides
can also harm other, beneficial insects. The insects also evolve resistance to the
pesticides.
Genes that code for the production of a protein derived from the bacterium
Bacillus thuringiensis, called Bt toxin, have been inserted into maize, cotton and
tobacco plants. The plants therefore produce the protein, which is converted
into the toxin once inside the gut of insects that have eaten the leaves. This
means that the toxin kills insects that feed on the plants, but not other insects.
Benefits include the following:
l There is less loss of the crop to insect pests, so greater yields are obtained.

l Less or no insecticide needs to be sprayed on the crop, reducing harm to
non-target insect species.
l Only insects that eat the plant are harmed, not other insects, which are
affected when insecticides are sprayed on crops.
l It is less likely that insect pests will evolve resistance to the Bt toxin than to
pesticides. However, there are signs that resistance can develop in some pest
species. This can be counteracted by using slightly different forms of the Bt
toxin, or a combination of two different Bt toxins, in GM crops.
Possible detrimental effects include the following:
l The seed of Bt corn and cotton costs more for farmers to buy than non-
GM seed, making it difficult for farmers in developing countries to afford it,
and therefore making it difficult for them to compete with farmers in richer
countries.
l It is possible that some non-pest insects might be harmed by the Bt toxin.
However, overall it is found that there are more non-pest insects present
in fields where Bt corn or Bt cotton are grown than in fields where non-Bt
crops are grown and sprayed with pesticides.
l Genes from the Bt crops might be transferred (e.g. in pollen) to species of
wild plants growing nearby. However, no evidence has been seen of this
happening to a significant degree.
Herbicide-resistant oilseed rape

Oilseed rape, Brassica napus, is grown for the oil that can be extracted from
its seeds. This is used in food production, and also to produce biofuels and
lubricants. GM varieties of oilseed rape contain genes that make the plants
resistant to the herbicide glyphosate. This means that farmers can spray the
field with herbicide, which will kill weeds but not the crop. Growing GM oilseed
rape therefore increases yields and reduces costs. A possible detrimental effect is
that the herbicide resistance genes could be transferred to species of wild plants
growing nearby, but so far little evidence of this has been detected.
GM salmon
Salmon are farmed for food in many parts of the world. A company in the USA
has developed a GM variety of salmon that contains a growth hormone gene
from another species of salmon, and a promoter from a different species of fish.
This makes the GM salmon grow much faster and larger than normal. This could
allow more food to be produced more quickly. However, there are concerns that,
if these fish escape from the cages where they are kept, they could reproduce in
the wild and adversely affect populations of other marine organisms. To avoid
these problems, it is planned to farm only sterile female fish. Moreover, the GM
salmon are all triploid, making it difficult for them to produce viable gametes. It
is also recommended that the fish are only grown in land-based water tanks, so
that they are much less likely to escape into the wild.

A level experimental skills and
investigations
Skills and mark allocations

More than one quarter of the marks in your A level examinations are for
practical skills. These are assessed in a written paper — not by a practical
examination. However, you will need to do plenty of practical work in a
laboratory throughout your A level biology course in order to develop the
practical skills that are assessed.
There is a total of 30 marks available on this paper. Although the questions are
different on each practical paper, the number of marks assigned to each skill is
always the same. This is shown in the table below.
Skill Total marks Breakdown of marks

Planning 15 marks Defining the problem 5 marks
Methods 10 marks
Analysis, 15 marks Dealing with data 8 marks
conclusions and Evaluation 4 marks
evaluation
Conclusion 3 marks
The syllabus explains each of these skills in detail, and it is important that you
read the appropriate pages in the syllabus so that you know what each skill is,
and what you will be tested on.
The next few pages explain what you can do to make sure you get as many
marks as possible for each of these skills. They have been arranged by the kind of
task you will be asked to do.
Some of the skills are the same as for AS. However, most of them are now a
little more demanding. For example, in dealing with data, you are not only
expected to be able to draw results charts and graphs, but also to use statistics
to determine what your results show.
How to make the most of

your practical skills
Planning Revised
At least one of the questions on the practical paper will require you to plan
an investigation.
The question will describe a particular situation to you, and ask you to design
an experimental investigation to test a particular hypothesis or prediction. The
planning question will usually involve investigating the effect of one factor (the
independent variable) on another (the dependent variable).
Sometimes, the question will tell you the apparatus you should use. In other
cases, you will need to decide on the apparatus. If you have done all of the

practicals listed in the syllabus during your course, then you will have had direct

experience of a wide range of apparatus and techniques, which will make this
much easier for you.
Stating a hypothesis
You may be asked to state a hypothesis. For example, you might be asked
to plan an experiment to find out how light intensity affects the rate of
photosynthesis. Your hypothesis could be:
Increasing light intensity will increase the rate of photosynthesis.
Notice that your hypothesis should include:
l reference to the independent variable (light intensity)
l reference to the dependent variable (rate of photosynthesis)
l a clear statement of how a particular change in the independent variable
(an increase) will affect the dependent variable (an increase)
You could be asked to express your hypothesis in the form of a sketch graph.
Here, you could draw a graph like this:
Rate of
photosynthesis
Light intensity
Your hypothesis should be:
l quantifiable — this means that you should be able to measure changes in
the independent and dependent variables and obtain numerical results
l testable — this means that you should be able to do an experiment that
will test whether or not your hypothesis could be correct
l falsifiable — this means that you should be able to do an experiment
whose results could show that your hypothesis is not correct
Notice that it is not possible to actually prove that a hypothesis is correct. For
example, if your experiment involved putting a piece of pondweed in a tube
and measuring the rate at which oxygen is given off with a lamp at different
distances from the tube, you might find that the rate of photosynthesis does
indeed increase with light intensity. You could say that your results ‘support’
your hypothesis. However, you cannot say that they ‘prove’ it, because you
would need to do more experiments and obtain a lot more data before you
could be sure this relationship is always true. Expert tip
However, you can disprove a hypothesis. If you kept on increasing the light Check exactly what the question is
intensity in this situation, you would probably find that the rate of asking you do. You may be expected
to state your hypothesis both in words
photosynthesis eventually levels off, as some other factor begins to limit the rate. and in the form of a predicted graph.
This would disprove your hypothesis.
Variables
You will usually need to decide what are the independent and dependent Expert tip
variables. You will already have thought about this for AS, and it is described Even if you are not asked to write
on p. 77. them down, make sure that you
have identified the independent and
You will also need to identify important variables that should be controlled or dependent variables. State clearly
standardised. This is described on p. 79. which variables you would control, and
be prepared to state exactly how you
Range and intervals would do this.
You will need to specify a suitable range and suitable intervals for the
independent variable that you would use. This is described on p. 77.
A level experimental skills and investigations 181

Apparatus
Sometimes you will be told exactly what apparatus you should use. If so, then
it is important that your experiment does use this apparatus. If you describe a
different kind of apparatus, you will not get many marks.
For example, the question may include a diagram of a plant shoot in a test tube
containing dye solution, with a layer of oil on the liquid surface. The question
may ask how you could use this apparatus to find the rate of movement
of water up the stem. You will probably never have used this apparatus for
this purpose, but you should be able to work out a way of using it for this
investigation. If you describe the use of a different piece of apparatus — for
example, a potometer — then you will not get many marks, because you have Expert tip
not answered the question. Take care that your plan uses the
If you do plenty of practical work during your biology course, then you apparatus that the question asks you
will become familiar with a wide range of apparatus. There is a list of basic to use, even if you think this is not the
best apparatus, or if it is not apparatus
apparatus that you should be able to use in the syllabus, under the heading that you have used before.
‘Laboratory equipment’.
Method
You will need to describe exactly what you will do in your experiment.
Changing and measuring the independent variable

You should be able to describe how you will change the independent variable
and how you will measure it. If the independent variable is the concentration of
a solution, then you should be able to describe how to make up a solution of a
particular concentration.
To make up a solution of 1 mol dm−3, you would place 1 mole of the solute in
a 1 dm3 flask, and then add a small volume of solvent (usually water). Mix until
fully dissolved, then add solvent to make up to exactly 1 dm3. (1 mole is the
relative molecular mass of a substance in grams.)
To make up a 1% solution, you would place 1 g of the solute in a container with
a small volume of solvent. Mix until fully dissolved, then make up to 100 cm3
with solvent.
You should be able to use a stock solution to make serial dilutions. This is
described on p. 78. It is worth remembering that a small test-tube holds about
15 cm3, and a large one (sometimes called a boiling tube) up to 30 cm3.
Keeping key variables constant

You will be expected to describe how you will control a particular variable. For
example, if you need to control temperature, then you should explain that you
would use a water bath (either thermostatically controlled, or a beaker of water
over a Bunsen burner) in which you take the temperature using a thermometer.
Measuring the dependent variable

You should describe how you will measure the dependent variable, and when
you will measure it.
Sequence of steps
Your method should fully describe the sequence of the steps you will carry out.
This will include setting up the apparatus and all the other points described
above relating to changing, measuring and controlling variables. You may like to
set this out as a series of numbered points.
Risk assessment
You should be able to identify any ways in which your experiment might cause
a risk of injury or accident, and to suggest safety precautions related to these
risks. Sometimes these are obvious — for example, if you are using concentrated

acid, then there is a risk that this might come into contact with clothing, skin

or eyes, and therefore protective clothing and eye protection should be worn.
In the experiment investigating the effect of light intensity on the rate of Expert tip
photosynthesis, care should be taken not to get water on the electrical light Always think about risk. Identify any
source, as this could cause a current surge or give a person an electric shock. genuine risks and explain how you
would minimise them. Do not invent
Sometimes, an experiment might be so simple and safe that there are no risks if there are none.
genuine risks. If so, then you should say so. Do not invent risks if there are none!
Reliability Expert tip

As described on p. 82, it is a good idea to do at least three repeats for each value
It is almost always a good idea to
of the independent variable. A mean can then be calculated, which is more likely suggest doing at least three repeats.
to give a true value than any one of the individual values.
Recording and displaying data, and drawing conclusions

This is the same as for AS. It is described on pp. 81–87.
Dealing with data Revised
Tables and graphs

You may be asked to draw tables or graphs to record and display data. These are
described on pp. 81–86.
Calculations to summarise or describe data

You will be asked to carry out some type of calculation, using data that you
have been given.
Mean, median and mode

These are best explained using an example. Let’s say that you had measured the
lengths of 20 leaves. Your results, recorded in mm, were:
33.5, 56.5, 62.0, 75.0, 36.0, 54.5, 43.5, 41.5, 54.0, 53.0
53.5, 39.0, 72.5, 66.5, 58.5, 42.5, 41.5, 49.0, 69.0, 38.5
(Notice that all the measurements are to three significant figures, or one decimal
place, and that all values have been measured to the nearest 0.5 mm.)
To calculate the mean value, add up all the values and divide by the total
number of measurements.
sum of individual values 1040
mean value = = = 52.0
number of values 20
Now let’s say you measured the lengths of 80 more leaves, and obtained the
results displayed in this histogram.
Number 25
of leaves
20
15
10
0
20 30 40 50 60 70 80 90
Leaf length/mm
A frequency histogram

The mode, or modal class, is the most common value in the set of
observations. In this instance, this is 50–54 mm. The median is the middle value
of all the values. In this case, the median value is 52.5 mm.
If the frequency histogram of your data is a perfect bell-curve, we say that the
data show a normal distribution. For a perfect normal distribution, the mean,
mode and median are all the same.
Percentage increase or decrease

You can remind yourself how to calculate these on p. 82.
Range and inter-quartile range

You have already met the idea of ‘range’, when thinking about the range of
values you would use for an independent variable in an investigation. The range
is the spread between the highest and lowest values in your data. For the 100
leaves in the histogram above, the range is 65 mm.
The inter-quartile range describes the range between the values that are one
quarter and three quarters along the complete range of your data. For the leaf
data, the inter-quartile range is from 36.25 to 68.75. These values tell you how
much the data are spread. If they are all very close to each other, then the range
and the inter-quartile range will be small.
This can be useful if you are looking at a set of repeat results in an experiment.
Ideally, you would hope that all the results are very close to one another. If they
are not, then this indicates a lack of reliability — there is a lot of variation in your
results for a particular value of your independent variable and therefore you
cannot have a great deal of faith that your mean is the ‘true’ value.
Statistics
Statistical methods are designed to help us to decide the level of confidence
we can have in what our results seem to be telling us. This is often a problem
in biology, where we are generally dealing with variable organisms and may not
be able to fully control all the control variables we would like. This means that
we do not get a set of results that precisely matches what we have predicted
from our hypothesis, or we are not really sure whether the set of results we have
obtained for one experiment is genuinely different from the set of results we
have obtained for another experiment.
In most statistical tests, you will need to calculate some basic values along the
way. These include standard deviation and standard error.
Calculating standard deviation

This is used with data that show an approximately normal distribution. It is a
measure of how much the data vary around the mean.
The larger the standard deviation, the wider the variation.
This is how you would calculate the standard variation for the leaf lengths
shown on p. 183. These were:
33.5, 56.5, 62.0, 75.0, 36.0, 54.5, 43.5, 41.5, 54.0, 53.0
53.5, 39.0, 72.5, 66.5, 58.5, 42.5, 41.5, 49.0, 69.0, 38.5
1 Calculate the mean. This is 52.0.
2 For each measurement, calculate how much it differs from the mean, and
then square each of these values. It is easiest to do this if you set the results
out in a table. In the table:
l x represents an individual value
−
l X represents the mean
l ∑ represents ‘sum of’

− −

x (x − X ) (x − X )2
33.5 −18.5 342.25
56.5 4.5 20.25
62.0 10.0 100.00
75.0 23.0 529.00
36.0 −16.0 256.00
54.5 2.5 6.25
43.5 −8.5 72.25
41.5 −10.5 110.25
54.0 2.0 4.00
53.0 1.0 1.00
53.5 1.5 2.25
39.0 −13.0 169.00
72.5 20.5 420.25
66.5 14.5 210.25
58.5 6.5 42.25
42.5 −9.5 90.25
41.5 −10.5 110.25
49.0 −3.0 9.00
69.0 17.0 289.00
38.5 −13.5 182.25
−
∑(x − X )2 = 2966
3 Now you need to use the following formula. You do not need to memorise
this formula, because if you are asked to use it in the exam, you will always
be given it.
−
∑(x − X)2
s =√
n−1
where:
s is the standard deviation
n is the number of readings (which in this case was 20)
So:
2966
s=√ = 12.49
19
This should be rounded up to 12.5.
All the values used in the calculations are in mm, so the standard deviation is
also in mm. You can say that the mean length of the leaves in this sample is
52.0 mm, with a standard deviation of 12.5. This is often written as: 52.0 mm ±
12.5 mm.
Calculating standard error

The set of data about leaf lengths was taken from a sample of leaves. How
likely is it that the mean of these data is actually the true mean for the entire
population? We can get some idea of this by calculating the standard error for
these data. The standard error tells us how far away the actual mean for the
entire population might be from the value we have calculated for our sample.
The formula for standard error, SM, is:
SM = s
√n
For the leaf data, this works out as:
12.5 = 12.5 = 2.8 mm
√20 4.5

This tells us that, if we took a fresh sample of leaves from the same population,
we could be 95% confident that the mean length would be within 2 × 2.8 mm
of our original mean, which was 52.0 mm.
We could use this to show 95% confidence limits on a graph. Let’s say you were
trying to find out if the lengths of leaves from a species of tree growing at the
edge of a wood were different from the lengths of leaves from the same species
growing in the middle of a wood. The first set of data (the ones you have been
looking at already) came from the edge of the wood. Here are the results you Now test yourself
got when you measured 20 leaves from the middle of the wood.
1 Show that the standard deviation
23.0, 46.5, 52.0, 32.5, 43.0, 50.5, 26.5, 31.5, 54.0, 28.0 for the leaves from the middle of
47.5, 39.0, 58.5, 47.5, 33.5, 42.5, 26.5, 51.0, 38.0, 38.5 the wood is 10.4 mm.
2 Show that the standard error for
The mean of these values is 40.5 mm. these data is 2.3 mm.
We can then calculate the standard deviation, which is 10.4 mm. The standard Answers on pp.206–207
error is 2.3 mm. Tested
The values for standard error can be shown as error bars on a bar chart.
Mean leaf 60
length/mm
50
Error bar
40
30
20
10
0
Population 1 Population 2
If the values for the two error bars overlap, this means there is not a significant
difference between the mean lengths of the two populations of leaves. Here,
there is no overlap, so we can say that it is possible that there is a significant
difference between the lengths of the leaves from the middle and the edge of
the wood.
The chi-squared test

This test is used to tell you whether any difference between your observed and
expected values is due to chance, or whether it means that your null hypothesis
cannot be correct. It can also be used to test for whether there is an association
between two variables — for example, whether people who are left-handed are
more likely to study physics A level than people who are right-handed. The chi-
squared test is described on pp. 150–151.
The t-test
This test is used to tell you whether the means of two sets of values, each
taken from a population of data that has a normal distribution, are significantly
different from one another.
We can use the leaf length data to illustrate how this test is done, and what it
tells us.

Here are the two sets of data again:

leaves from the edge of the wood
33.5, 56.5, 62.0, 75.0, 36.0, 54.5, 43.5, 41.5, 54.0, 53.0
53.5, 39.0, 72.5, 66.5, 58.5, 42.5, 41.5, 49.0, 69.0, 38.5
leaves from the middle of the wood
23.0, 46.5, 52.0, 32.5, 43.0, 50.5, 26.5, 31.5, 54.0, 28.0
47.5, 39.0, 58.5, 47.5, 33.5, 42.5, 26.5, 51.0, 38.0, 38.5
Just as for the chi-squared test, you begin by constructing a null hypothesis. This
would be:
There is no significant difference between the means of the lengths of the leaves
from the edge of the wood and from the middle of the wood.
The formula for the t-test will always be given to you, so you do not need to
learn it. It is:
|x − x |
t = 12 2 2
s1 s2
+
√ n1 n2
The symbols all mean the same as before. We have already calculated these
values. They are as follows:
x1 is the mean for the first set of leaves, 52.0 mm.
x2 is the mean for the second set of leaves, 40.5 mm.
s1 is the standard deviation for the first set of leaves, 12.5 mm.
s2 is the standard deviation for the second set of leaves, 10.4 mm.
n1 and n2 are the numbers in each sample, which were both 20.
Substituting these values into the formula, we get:
|52 − 40.5|
t=
12.52 + 10.42
√ 20 20
11.5
t=
156.25 + 108.16
√ 20 20
t = 3.16
What does this mean? You now have to look up this value of t in a table of
probabilities.
First, you need to decide how many degrees of freedom there are in your data.
To do this, use the formula:
degrees of freedom = n1 + n2 − 2
= 20 + 20 − 2
So the number of degrees of freedom is 38.
Here is a small part of the table showing values of t.
Degrees of Probability that null hypothesis is correct

freedom 0.10 0.05 0.02 0.01
25 1.70 2.06 2.49 2.79
30 1.70 2.04 2.46 2.75
40 1.68 2.02 2.42 2.70

Your value of t is 3.16. The table does not have a row for 38 degrees of freedom,
so we can look at the closest one, which is 40. There is no value in this row that
is as high as your value for t. The values in the table are increasing from left to
right, so we can say that our value of t would lie well to the right of anything in
the table. This means that the probability of the null hypothesis being correct is
much less than 0.01.
Just as we did for the chi-squared test (pp. 150–151), we take a probability of 0.05
as being the decider for the t-test. If the probability is lower than this, then we
can say it is very unlikely that the null hypothesis is correct. In other words, there
is a significant difference between the mean lengths of the two populations of
leaves. The mean length of the leaves in the middle of the wood is significantly
less than the mean length of the leaves at the edge of the wood.
Spearman’s rank correlation

This test is used to test for a correlation between two sets of data that are
not distributed normally. You can use this test, for example, to see if there is a
correlation between the distribution and abundance of two species in a habitat.
In order to use the test, you should have:
l two sets of data where the data points are independent of each other
l ordinal data — that is, data that consist of numerical scores that can be
ranked
l drawn a scatter diagram of one set of data against the other, and it looks as
though they might be correlated
l ideally, between 10 and 30 pairs of observations
l data that were collected randomly within the population
The formula for Spearman’s rank correlation, rs, is:

6 × ΣD2
rs = 1 − 3
n −n
where n is the number of pairs of observations, and D is the difference between
each pair of ranked measurements.
For example, imagine you have used quadrats to sample two species, X and Y,
on a rocky shore. You have counted the number of each species in each quadrat.
Here are your results:
Quadrat Number of species X Number of species Y

1 14 33
2 8 4
3 22 41
4 1 0
5 16 20
6 5 4
7 12 23
8 7 16
9 9 21
10 19 38
You think that your data suggest that there might be a correlation between the
abundance of the two species — i.e. high numbers of species X appear to be
associated with high numbers of species Y.

First, rank the data for each of the species. The largest number has rank of 1:

Number of Rank for Number of Rank for
Quadrat species X species X species Y species Y
1 14 4 33 3
2 8 7 4 8=
3 22 1 41 1
4 1 10 0 10
5 16 3 20 6
6 5 9 4 8=
7 12 5 23 4
8 7 8 16 7
9 9 6 21 5
10 19 2 38 2
Now calculate the differences in rank between the two species, and square it:
Rank for species Rank for species Difference in

Quadrat X Y rank, D D2
1 4 3 1 1
2 7 8= −1 1
3 1 1 0 0
4 10 10 0 0
5 3 6 3 9
6 9 8= 1 1
7 5 4 1 1
8 8 7 1 1
9 6 5 1 1
10 2 2 0 0
ΣD2 = 15
Substitute into the equation:

6 × 15
rs = 1 − 3
10 − 10
90
=1−
990
= 1 − 0.09 Now test yourself
= 0.91
3 A student recorded the numbers
The closer this number is to 1, the greater the degree of correlation between of species P and species Q in 10
the two sets of data. You may be given a table like this, against which you can quadrats. The results are shown
compare your calculated value, to see if the correlation is significant. If your in the list. In each case, the first
value for rs is equal to or greater than the number in the table, then there is a number is the number of species
P, and the second number is
significant correlation. species Q.
n 5 6 7 8 9 10 11 12 14 16
6, 5; 12, 22; 1, 3; 0, 4; 16, 35; 8,
10; 11, 11; 4, 9; 5, 3; 12, 21
Critical 1.00 0.89 0.79 0.76 0.68 0.65 0.60 0.54 0.51 0.51
Use Spearman’s rank correlation
value
to determine whether there
of rs
is a correlation between the
distribution of species P and
For these two sets of data, n is 10 and rs is 0.91, which is much greater than the species Q.
critical value of 0.65. So we can say that that there is a correlation between the Answers on p.207
abundances of the two species. Tested

Pearson’s linear correlation
This test is used when you think there may be a linear correlation between two
sets of normally distributed data. You can use it when:
l you have two sets of continuous data — for example, reaction time and
volume of coffee drunk; numbers of species A and numbers of species B;
mass of seed and time it takes to fall to the ground
l you have drawn a graph with one set of data on the x-axis and the other set
on the y-axis, and it looks as though you could draw a straight line through
the points
l the data have come from a population that is normally distributed
l you have at least five sets of paired data
The formula for Pearson’s linear correlation, r, is:

Σ xy – nx y
r=
n sx sy
where:
x and y are the individual values of your results (observations)
x and y are the means of the two sets of observations
n is the number of observations
sx and sy are the standard deviations for x and y
If your calculation gives you r = 1 or −1, then the correlation is perfectly linear.
This means that, if you plotted one set of data on the x-axis and the other set
on the y-axis of a graph, every point would lie exactly on a straight line.
These are the steps you need to follow:
1 Draw a table and fill in all your results for x and y, with ‘matching’ pairs
opposite one another, just like the first one for Spearman’s rank correlation
on p. 188.
2 Calculate the value of xy for each pair of data, by multiplying each pair. You
can do this in an extra column on the right of your table. Expert tip
3 Add up all your values of xy to find Σ xy. Note that, even if Spearman’s rank
4 Calculate the means, x and y. correlation or Pearson’s linear
correlation test shows that there is
5 Calculate nx y. a significant correlation between
6 Calculate the standard deviation for each set of data (see p. 185). (You may your two sets of data, this does not
have a calculator that can do this quickly, or you can find a website that does mean that one is causing the other.
it for you — you just have to key in all of your data and press a button.) Correlation does not necessarily imply
a causal relationship.
7 Now substitute all of your numbers into the formula above.

4 A student measured the length of the shell of 10 molluscs, and their body mass. These are her results:
Shell length/mm Body mass/g

38 6.1
18 3.6
20 3.2
31 5.7
12 2.6
12 3.2
25 4.7
20 3.6
29 4.7
19 3.3
Use Pearson’s linear correlation test to determine whether there is a linear correlation between shell length and body mass.
Answer on p.207
Evaluation
This skill builds on what you have already been doing at AS. You need to be Expert tip
able to: During your course:
l spot anomalous data. These are values that do not fit into a strong l Take every opportunity to practise
pattern that is shown by the rest of the data. This may indicate that you calculating and using statistical
made a mistake when taking a measurement, or that some other variable tests.
l Make you sure you know how to
had changed significantly for that one reading. The best thing to do with
calculate the number of degrees of
genuinely anomalous data is to take them out of your results. Do not include freedom.
them in calculations of means, and do not take any notice of them when l The most important part of a
deciding where to draw a line on a graph. statistical test is deciding what you
l consider the need for repeats or replicates. If you have decided that can conclude from it. Make sure
you get plenty of practice in doing
a data point is anomalous, then it would be a good idea to take repeat
this during your course.
readings for that value. Only by repeating those readings can you be sure
In the exam:
that your odd result really is anomalous. In any case, it is often a good idea to l If asked, show clearly how you
include a set of repeats or replicates, as explained on pp. 82–83 and 183. would use tables and graphs
l consider the need for an expansion of the range, or a change in the to display your data. Show the
interval, of the independent variable complete headings for rows,
columns and axes, including units.
l consider major sources of error in the investigation. These are discussed
l Show every step in each calculation
on pp. 87–88. You should be able to judge how much these might have that you are asked to do. Even if
affected the reliability of the results. you get the correct answer, you
will not get full marks if steps are
Drawing conclusions missing.
Again, this builds on what you did at AS, where you were also required to draw l Be prepared to suggest which
conclusions from data. Now, though, you may also have statistical analyses would be the best statistical test to
use in a particular situation.
to help you to do this. Remember that you will be expected to use the data l You will not normally be asked to
provided, or that you have calculated, to support your conclusion. work through an entire statistical
You may also be asked to give biological explanations of the data provided. So it test, but only part of it. Read the
information you are given very
is important that you go into the examination with the facts and concepts in carefully.
your head that you have learned throughout your AS and A level course.

A level exam-style questions
and answers
This practice paper comprises structured questions similar to Student A
those you will meet in the exam.
(a) Compound 1 is fructose phosphate 7. Compound 2 is
You have 2 hours to do the paper. There are 100 marks on pyruvate ✓.
the paper, so you can spend just over 1 minute per mark. 85
marks are for structured questions, and there is one 15-mark Compound 1 is a bisphosphate, not a phosphate (it has
question that requires more extended writing. You will get a two phosphate groups attached to it). Mark: 1/2
choice of one from two questions in the actual exam paper,
but in this sample paper there is only one question. Student A
(b) Cytoplasm ✓.
See p. 91 for advice on using this practice paper.
Correct. Mark: 1/1
Exemplar paper Student A

(c) (i) This is anaerobic respiration. The pyruvate is changed
Question 1 into lactate so it does not stop glycolysis happening.
The flow diagram shows part of the metabolic This does not answer the question. Mark: 0/2
pathway of glycolysis.
Student A
Hexose sugar (e.g. glucose)
(ii) It goes to the liver, which breaks it down ✓. This
2 ATP
needs oxygen, which is why you breathe faster than
2 ADP usual when you have done a lot of exercise.
Compound 1
Much more is needed for full marks. The comment
about breathing rate is correct but is not relevant to this
Triose phosphate Triose phosphate particular question. Mark: 1/2
NAD 2 ADP + Pi NAD 2 ADP + Pi Student B

Reduced 2 ATP Reduced 2 ATP (a) Compound 1 is hexose bisphosphate ✓. Compound 2 is
NAD NAD pyruvate ✓.
Compound 2 Compound 2
Both are correct. Mark: 2/2
(a) Name compound 1 and compound 2. (2 marks)

(b) State the part of the cell in which this metabolic Student B
pathway takes place. (1 mark) (b) Cytoplasm ✓
(c) (i) Describe how compound 2 is converted to
lactate in a human muscle cell if oxygen is Correct. Mark: 1/1
not available in the cell. (2 marks)
(ii) Describe what happens to the lactate
produced. (2 marks) Student B
(Total: 7 marks) (c) (i) It is combined with reduced NAD ✓, which is
oxidised back to ordinary NAD. The enzyme lactate
dehydrogenase ✓ makes this happen.
This is completely correct. Mark: 2/2

A level exam-style questions and answers
Student B
1000
Carbon dioxide uptake/

(ii) The lactate diffuses into the blood and is carried
Grown in
to the liver cells ✓. They turn it back into pyruvate bright light
again ✓, so if there is oxygen it can go into a 800
mitochondrion and go through the Krebs cycle. Or
μmol dm−2 h−1

the liver can turn it into glucose again ✓, and maybe 600
store it as glycogen.
400
All correct and relevant. Mark: 2/2
Grown in
200 dim light
Question 2 0
5 10 15 20 25 30
The diagram shows the structure of a chloroplast. −200
Light intensity/mJ cm−2 s−1
(i) Compare the effect of light intensity on

the two groups of plants between 0 and
30 mJ cm−2 s−1. (4 marks)
(ii) Suggest why the plants gave out carbon
A dioxide at very low light intensities.
(2 marks)
B (iii) Suggest two differences in the two groups
of plants that could have been caused by
C their exposure to different intensities of
light as they were growing, and that could
D help to explain the results shown in the
graph. (2 marks)
E
(Total: 13 marks)
Student A
(a) Give the letter of the part of the chloroplast
where each of the following takes place. (a) (i) E ✓
(i) fixation of carbon dioxide (1 mark) (ii) D ✓
(ii) the light dependent reactions (1 mark)
(b) A grass adapted for growing in a tropical Both correct. Mark: 2/2
climate was exposed to low temperatures for
several days. The membranes of part D moved
Student A
closer together, so that there was no longer
any space between them. This prevented (b) It would not be able to make any ATP ✓, which is needed
photophosphorylation taking place. for the Calvin cycle ✓. Without the Calvin cycle it would
not be able to make carbohydrates.
Explain how this would prevent the plant from
synthesising carbohydrates. (3 marks)
This is correct, but lacking in detail. Mark: 2/3
(c) Two groups of seedlings were grown in identical
conditions for 2 weeks. One group was then
grown in high-intensity light and the other Student A
group in low-intensity light, for 4 weeks.
(c) (i) Neither of the groups took up any carbon dioxide
Each group of plants was then placed when there was no light ✓ Then the quantity of
in containers in which carbon dioxide carbon dioxide increased dramatically for the bright
concentration was not a limiting factor. They light plants, and slowly for the dim light plants.
were exposed to light of varying intensities and Then it levelled out, lower for the dim light plants
their rate of carbon dioxide uptake per unit of than for the bright light ones ✓. The dim light plants
leaf area was measured. levelled out at 380 and the bright light ones at
The results are shown in the graph. 1000 µmol dm−2 h−1 ✓.
A level exam-style questions and answers 193

Some good comparative points are made here. This is good answer, with some comparative figures
However, a fundamental error is that the answer is quoted (with their units). Five credit-worthy points have
expressed as though the x-axis showed time — for example, been made, although there are only 4 marks available for this
the words ‘slowly’ and ‘then’ are used. It is also not a good question. Note the avoidance of any vocabulary that could be
idea to use words such as ‘dramatically’. See answer B for a associated with time. Mark: 4/4
better way of expressing these points. Mark: 3/4
Student B
Student A (ii) When the light intensity was very low, the plants
(ii) They could not photosynthesise ✓, so the carbon would not be able to photosynthesise so they
dioxide in their leaves just went back out into the would not take up any carbon dioxide ✓. However,
air again. they would still be respiring (they respire all the
time) so their leaf cells would be producing carbon
One correct point is made here. Mark: 1/2 dioxide ✓, which would diffuse out into the air.
Normally, the cells would take up this carbon
dioxide for photosynthesis.
Student A
(iii) The ones that had grown in the bright light could This is a good answer. Mark: 2/2
have bigger leaves and more chlorophyll. ✓
Student B
The suggestion about bigger leaves is not correct. Even
if the plants did have bigger leaves, this would not (iii) The plants grown in the light would probably be
affect the results, because the carbon dioxide uptake is a darker green because they would have more
measured per unit area (look at the units on the y-axis of the chlorophyll ✓ in their chloroplasts, so they would
graph). The second point is a good suggestion. Mark: 1/2 be able to absorb more light and photosynthesise
faster. They might also have more chloroplasts
Student B in each palisade cell. ✓ And leaves sometimes
produce an extra layer of palisade cells if they are in
(a) (i) E ✓
bright light. ✓
(ii) D ✓
This answer actually contains three correct points, and
Both are correct. Mark: 2/2
two of them have been explained, which was not
required. The student could have got 2 marks with a much
shorter answer. Nevertheless, this answer shows good
Student B
understanding of the underlying biology. Mark: 2/2
(b) No ATP would be made ✓ in the light dependent
reaction, so there would not be any available for the
light independent reactions, where carbohydrates (triose Question 3
phosphate) are made in the Calvin cycle ✓. ATP is needed
to convert GP to triose phosphate ✓ (along with reduced A group of islands contains three species of mice,
NADP) and also to help regenerate RuBP ✓ from the triose each species being found on only one island. A
phosphate so the cycle can continue.
fourth species is found on the mainland. A region
of the DNA of each species was sequenced, and the
All correct and with good detail. Mark: 3/3
percentage differences between the samples were
calculated. The results are shown in the table.
Student B
Mainland Island A Island B
(c) (i) Below about 0.5 light intensity, both groups gave out
carbon dioxide ✓. As light intensity increased, the Island A 6.1
amount of carbon dioxide taken up by the plants Island B 4.8 9.7
grown in bright light increased more steeply ✓
Island C 5.2 10.3 7.5
than for the ones grown in dim light. In the group
grown in dim light, the maximum rate of carbon
dioxide uptake was 380 µmol dm−2 h−1, whereas (a) Discuss how these results suggest that the
for the ones in bright light it was much higher ✓, at species of mouse on each island has evolved
1000 µmol dm−2 h−1 ✓. For the bright light plants, the from the species on the mainland, and not from
maximum rate of photosynthesis was not reached one of the other island species. (5 marks)
until the light intensity was 25 mJ cm−2 s−1, but for (b) Each of these four species of mice is unable to
the ones grown in dim light the maximum rate was breed with any of the other species, even if they
reached at a lower ✓ light intensity of 20 mJ cm−2 s−1. are placed together.

The answer begins with a clearly explained link between

Suggest how reproductive isolation between speciation and reproductive isolation, and then goes on
the mice could have arisen, and explain its role to describe how two populations could become
in speciation. (5 marks) reproductively isolated. Mark: 5/5
(Total: 10 marks)
Question 4
Student A
(a) The three island mice each have DNA more similar to the Gibberellin is a plant hormone (plant growth
mainland mouse than to each other ✓✓. So they have regulator) that is involved in stem elongation and
probably all evolved from the mainland mouse. If one of the seed germination.
island mice had evolved from another island mouse, their (a) Explain why plants with the genotype lele are
DNA would be more similar. ✓ dwarf, whereas plant with genotype LeLe or Lele
can grow tall. (4 marks)
This answer shows that the student has managed to (b) During the germination of a wheat seed,
work out what the table shows, but it does not provide gibberellin activates the production of the
enough detail to get all of the marks available. Mark: 3/5 enzyme amylase.
(i) Explain how it does this. (4 marks)
Student A
(ii) Outline the role of amylase in seed
(b) If they are on different islands, they will have different germination. (2 marks)
selection pressures ✓ so the mice might end up different.
They might have different courtship behaviour ✓, so they (Total: 10 marks)
will not be able to mate with each other ✓. You have to get
reproductive isolation to produce a new species. Student A
(a) Le is dominant to le, so the heterozygote Lele can make
This is a reasonable description. The last sentence is gibberellin, but lele cannot ✓.
moving towards another mark but it really only repeats
what is already in the question. Mark: 3/5
This is correct, but there is no explanation of how allele
Student B Le or le are involved in the production of gibberellin.
The answer does not make any link between the production
(a) From the table, we can see that each island mouse’s DNA of gibberellin and the ability to grow tall. Mark: 1/4
is more similar to the DNA of the mainland mouse than
to any of the other island mice. ✓ For example, the island Student A
C mice have DNA that is 10.3% different from the island
A mice and 7.5% different from the island B mice, but only (b) (i) Gibberellin (GA) is made by the seed when it is warm
5.2% different from the mainland mice. ✓ The longer ago and it takes up water. The GA breaks the dormancy
two species split away from each other, the more different of the seed. The GA makes the cells in the seed
we would expect their DNA to be. This is because the secrete amylase.
longer the time, the more mutations ✓ are likely to have
occurred, so there will be different base sequences ✓ in the Once again, the student has written nothing wrong, but
DNA. there is no description of how gibberellin brings about
amylase synthesis. Mark: 0/4
This is a good answer to a difficult question. Good use
Student A
is made of the data in the table, and some of the figures
are used to support the answer. The last part of the answer (ii) Amylase breaks down starch to maltose. ✓
explains why differences in DNA base sequence indicate
degree of relationship. Mark: 5/5 This is correct but it is not enough for an answer to an
A level question. Mark: 1/2
Student B
(b) A species is defined as a group of organisms that can Student B
interbreed with each other to produce fertile offspring. ✓ (a) The gene Le/le codes for the synthesis of an enzyme that
So to get new species you have to have something that is needed to make gibberellin. ✓ Le codes for a functioning
stops them reproducing together so genes cannot flow ✓ enzyme, but le does not. ✓ Le is dominant and le is
from one species to the other. This might happen if recessive, so the plant only needs one Le allele to be able to
different selection pressures ✓ acted on two populations make the enzyme. ✓ Gibberellin is needed to make plants
of a species, so that different alleles were selected for ✓ grow tall because it simulates stem elongation ✓, so plants
and over many generations their genomes became more with the genotype lele do not make any gibberellin ✓ and
different ✓. So they might be the wrong size and shape ✓ do not grow tall.
to be able to breed with each other.

This is a good answer. It explains each step of the
process by which the allele Le allows plants to grow tall. possible to use more because in many cases this
Mark: 4/4 was the largest number that could be spared from
the seed bank. In most cases, the germination rate
Student B of the seeds had already been tested when they
(b) (i) Before the seed starts to germinate, proteins called were first collected in 1994, so a comparison was
DELLA proteins are bound to transcription factors possible with the germination rates in 2001 after 7
inside the cells in the seed. ✓ When a seed takes years of storage.
up water, this stimulates the synthesis of GA by
the tissues of the embryo plant. GA combines with The graphs show the results for two species,
a receptor ✓ inside the cells in the seed, and also Asparagus officinalis and Sanguisorba officinalis.
with an enzyme. This activates the enzyme ✓ so
that it can break down the DELLA protein ✓. The Asparagus officinalis
transcription factor is now free to bind with DNA ✓ Percentage 100
in the cell, which initiates the transcription of genes cumulative
germination 80
for amylase ✓.
60
Here is another excellent answer. The series of events is
described well and good use is made of technical 40
language, such as ‘synthesis’ and ‘transcription factor’. Mark:
4/4 20
0
Student B 0 20 40 60 80 100
(ii) The amylase hydrolyses starch stored in the Time/days
endosperm, producing maltose ✓, which is soluble Sanguisorba officinalis
and is transported to the embryo for use as an Percentage 100
energy resource ✓ and also as raw material from cumulative
which cellulose can be synthesised to make new germination 80
cell walls ✓.
60
This answer has plenty of relevant detail, and scores 40

maximum marks. The student tells us what amylase
does (again making good use of technical language) and how 20
this is important for growth of the embryo. Mark: 2/2
0
0 20 40 60 80 100
Question 5 Time/days
Freshly collected seeds

The Irish Threatened Plant Genebank was set up in Seeds stored for 7 years
1994 with the aim of collecting and storing seeds
from Ireland’s rare and endangered plant species. (b) (i) Compare the germination rates of stored
The natural habitat of many of these species is and fresh seeds of Sanguisorba officinalis.
under threat. For each species represented in (3 marks)
the bank, seeds are separated into active and (ii) Compare the effect of storage on the
base collections. The active collection contains germination rates of Sanguisorba officinalis
seeds that are available for immediate use, which and Asparagus officinalis.
could be for reintroduction into the wild, or for (3 marks)
germination to produce new plants and therefore (c) It has been suggested that species stored as
new seeds. The base collection is left untouched. seeds in seed banks have different selection
Some of the base collection is kept in Ireland, and pressures acting on them compared with the
some is kept at seed banks in other parts of the same species living in the wild.
world. (i) Explain why the selection pressures in a
(a) Suggest why seed banks separate stored seeds seed bank and in the wild are likely to be
into active and base collections. (2 marks) different. (2 marks)
In 2001, an investigation was carried out into the (ii) Suggest how the possible harmful effects of
effect of long-term storage on the ability of the these differences could be minimised.
seeds to germinate. Fifteen species were tested. (5 marks)
In each case, 100 seeds were tested. It was not (Total: 15 marks)

Student A Student B
(a) So they always have some spare. (a) Having active collections is good because it means there
are seeds available that can be used for something. But you
This is not enough for a mark. Mark: 0/2 must always keep some seeds in storage, because the whole
point of a seed bank is that it stores seeds and these need
to be kept safe so they do not get destroyed. ✓ If all of
Student A them got used for growing plants, then perhaps the plants
(b) (i) The stored ones germinated better than the fresh would die ✓ and you would not have any seeds left.
ones. The stored ones go up more quickly than the
fresh ones. But they all end up at the same place, This is not very well expressed, but the right ideas are
about 80%. ✓ there. Mark: 2/2
Student B
This answer loses out by poor wording, and not being
clear enough about exactly what is being described. The (b) (i) The stored seeds germinated much faster ✓ than the
word ‘better’ in the first sentence could mean that the seeds fresh ones. By 10 days, about 60% of the stored seeds
germinated more quickly, or that more of them germinated, had germinated, but only about 8% ✓ of the fresh
so this needs to be clarified. ‘Go up more quickly’ is also not ones. By 50 days, all of the stored seeds that were
clearly related to germination. The last sentence is rather going to germinate (about 80%) had germinated.
generously awarded a mark for the idea that eventually about It took 100 days for 80% of the fresh seeds to
80% of the seeds in each batch germinated. Mark: 1/3 germinate. ✓ We cannot tell if any more would have
germinated after that because the line is still going up
Student A when the graph stops. ✓
(ii) The Sanguisorba seeds germinated better when
they had been stored, but the Asparagus seeds Clear comparative points have been made about the
germinated better when they were fresh. Storing speed at which germination happened, and also about
the Sanguisorba seeds made them germinate the maximum percentage of seeds that germinated. Mark:
faster, but the Asparagus seeds germinated 3/3
slower. ✓
Student B
Again, poor wording means that this answer only gets 1 (ii) Storage seemed to help the germination for
mark. The word ‘better’ is used in the first sentence Sanguisorba, but it made it worse for Asparagus.
and, as explained above, this is not a good word to use in this For Asparagus, storage made it germinate slower,
context. One mark is given for the idea that storage caused but it germinated faster for Sanguisorba. ✓ And
slower germination in Asparagus but faster germination in for Asparagus only about 25% of the stored seeds
Sanguisorba. Mark: 1/3 germinated compared with 60% of the fresh
seeds ✓, but with Sanguisorba about the same
Student A percentage of seeds germinated for both fresh and
(c) (i) The conditions in which the seeds grow might be stored ✓.
different in the seed bank from in the wild.
This is a good answer, which again makes clear
This is not quite correct because seeds do not grow. comparisons and discusses both the speed of
Growth only happens after germination, so it is germination and the percentage of seeds that eventually
seedlings and plants that grow. The student needs to think germinated. Mark: 3/3
more carefully about what happens to seeds in a seed bank.
Student B
Mark: 0/2
(c) (i) In the seed bank, the seeds are just stored. So the
Student A ones that survive are the ones that are best at
(ii) The seeds could be grown in conditions like those surviving in those conditions as dormant seeds. ✓
in the wild. In the wild, the plants have to be adapted to grow
in their habitat ✓, so maybe they have to have long
roots or big leaves or whatever. In the seed bank, that
Again, this has not been clearly thought out. Mark: 0/5
does not matter.
This does answer the question, but it could perhaps

have been written a little bit more carefully and kept
shorter. Mark: 2/2

Student B
Percentage 100
(ii) You might get seeds that are really good at activity
Normal
surviving in a seed bank but when they germinate
80 tyrosinase
they produce plants that are not very good at
surviving in the wild. To avoid this, you could keep
on collecting fresh seeds from plants in the wild ✓, 60
and only storing them for a little while before
replacing them with new ones ✓. If you had to store 40
seeds for a long time, you could keep germinating Tyrosinase
some of them ✓ and growing them in conditions from albino
20 girl
like in the wild ✓ and then collect fresh seeds from
the ones that grew best ✓.
0
20 30 40
This is a good answer to a tricky question. The student
Temperature/ºC
has made several sensible suggestions, including storing
the seeds for a shorter time and periodically exposing the (ii) Studies on the production and activity
plants to natural conditions where the ‘normal’ selection of tyrosinase in living cells found that
pressures will operate. Mark: 5/5 normal tyrosinase leaves the endoplasmic
reticulum shortly after it has been
Question 6 made, and accumulates in vesicles in
the cytoplasm where it becomes active.
However, the tyrosinase in the albino
Two parents with normal skin and hair colouring girl’s cells only did this at temperatures
had six children, of whom three were albino. of 31 °C or below. At 37 °C, her tyrosinase
Albino people have no colouring in their skin or accumulated in the endoplasmic reticulum.
hair, due to having an inactive form of the enzyme Use your answer to (i) and the information
tyrosinase. Tyrosinase is essential for the formation above to discuss explanations for the
of the brown pigment melanin. distribution of colour in the hair on
(a) The normal allele of the tyrosinase gene is A, different parts of the albino girl’s body.
and the allele that produces faulty tyrosinase (4 marks)
is a. (Total: 11 marks)
State the genotypes of the parents and their
albino children. (2 marks) Student A
(b) Albinism is a relatively frequent condition in
humans, but one of these albino children had a (a) Aa and aa ✓✓
very unusual phenotype. While most of her hair
was white, the hair of her eyebrows developed It is not clear which genotype refers to the parents and
which to the children, but the answer has generously
some brown colouring, as did the hair on
been given the benefit of the doubt. Mark: 2/2
her hands and lower legs. Genetic analysis
suggested that a mutation had occurred in the Student A
faulty tyrosinase allele.
(b) If it had been in one of her own cells, then only that cell
Suggest why it is likely that this mutation would be affected and not the whole body. ✓
occurred in the ovaries or testes of the girl’s
parents, rather than in her own body. This is correct, as far as it goes. Mark: 1/2
(2 marks)
(c) The graph opposite shows how the activity of
normal tyrosinase and tyrosinase taken from Student A
the albino girl were affected by temperature. (c) (i) Both enzymes get more active as temperature
(i) Compare the effects of temperature on increases. ✓ The normal enzyme is still getting more
normal tyrosinase and the albino girl’s active even at 45 °C. ✓ The girl’s enzyme has an
tyrosinase. (3 marks) optimum activity at 35 °C ✓ and above that it quickly
7 gets a lot less active.

The answer begins well with a sentence about both

Student B continued
enzymes. Two clear distinguishing points are then
made. However, the use of the term ‘quickly’ is inappropriate vesicles where it becomes active. This is why the
as there is nothing about time on the graph. ‘Steeply’ would hairs on her hands and legs are darker because the
have been better. Mark: 3/3 enzyme was able to make melanin there. ✓ But
in warmer places the enzyme stops working and
Student A cannot get out of the RER, so no melanin is made. ✓
(ii) Maybe her legs and eyebrows and hands were
colder ✓, like in a Himalayan rabbit. So the enzyme This is an excellent answer. Both sets of information
would not work well where it was hotter ✓ and (from the graph and the description about the
would not make melanin. endoplasmic reticulum) are used to provide an explanation
linked to what the student had already learned about the
control of hair colour in other situations. Mark: 4/4
This is a difficult question, and the student has done
well to think of what he/she had learned about the
interaction between genes and environment in animal hair Question 7
colour and recognise that it could relate to this situation.
However, the answer does not refer clearly to the information
provided, and this needs to be done in order to achieve more (a) Describe how gel electrophoresis can be used to
marks. Mark: 2/4 separate DNA fragments of different lengths.
(6 marks)
Student B (b) The diagram shows the results of
(a) Parents Aa and Aa. ✓ Albino children aa. ✓ electrophoresis on DNA samples taken from a
mother, her child and its alleged father.
This is correct and clear. Mark: 2/2
Explain what can be concluded
from these results. (3 marks)
Student B (Total: 9 marks)
(b) If it had occurred in her own body, then it would probably
be in only one cell ✓ so you would not see the effects in
cells in different parts of the body. If it had been in one of
the parents, then the mutated gene would have been in the
zygote ✓, so when the zygote divided by mitosis the gene
would get copied ✓ into every cell in her body.
This is absolutely correct. Mark: 2/2

Mother
Child
Alleged
father
Student B
(c) (i) The activity of both enzymes increases as
temperature increases from 22 °C to 35 °C. ✓ Student A
However, above that the activity of the girl’s enzyme (a) First you cut the DNA up into pieces using restriction
drops really steeply ✓ so it has only 50% activity at enzymes. ✓ Then you put the DNA onto some agarose
37 °C. But the normal enzyme keeps on increasing its gel ✓ in a tank and switch on the power supply so the
activity up to where the graph ends at 45 °C. ✓ At DNA gets pulled along the gel ✓. The bigger pieces move
38 °C it has 95% activity, almost twice as much as for more slowly, so they end up not so far along the gel as the
the girl’s enzyme. ✓ smaller pieces. ✓ You cannot see the DNA so you need to
stain it with something so it shows up.
This is a good answer, which mentions particular points
on the curves and provides a quantitative comparison There are no errors in this answer, but a little more
between the figures for the two enzymes at a particular detail is needed. Mark: 4/6
temperature (38 °C). Mark: 3/3
Student A
Student B
(b) The child has one band that is in its mother’s DNA and
(ii) The extremities of the girl’s body will be cooler than another that is in the alleged father’s DNA. ✓ So he could
other parts ✓, so the tyrosinase will be able to work be the father. ✓
here because according to the graph it can work
well up to 35 °C ✓. It will also be able get out of the
Two correct points are made. Mark: 2/3
endoplasmic reticulum ✓ in her cells and into the

Student B Student A
(a) The DNA is cut into fragments using restriction enzymes ✓, (b) Adrenaline binds to a receptor molecule in the cell
which cut it at particular base sequences. Then you place membrane. ✓ This activates a hormone 7 messenger
samples of the DNA into little wells in agarose gel ✓ in an called cyclic ADP 7, which binds to kinase ✓ proteins in
electrophoresis tank. A voltage is then applied ✓ across the the cytoplasm. This causes phosphate groups to be added
gel. The DNA pieces have a small negative charge so they to enzymes, which activates them. The activation of these
steadily move towards the positive ✓ terminal. The larger enzymes results in the activation of many molecules of
they are, the more slowly they move ✓ so the smaller ones glycogen hydrolase 7, which hydrolyse glycogen to glucose.
travel further ✓ than the big ones. After a time, the power
is switched off so the DNA stops moving. You can tell Two words are correct. This kind of question is often
where it is by using radioactivity ✓, so the DNA shows up as thought of as being easy, but that is far from the case
bands on a photographic film. — you need to choose the words carefully. Mark: 2/5
All the points made are correct and there is enough Student A
detail for full marks. Mark: 6/6 (c) One enzyme can break down a lot of molecules of
glycogen, because enzymes are not used up. ✓
Student B
(b) The top band for the child matches the top band for the One correct point is made. Mark: 1/3
mother ✓, and the bottom band for the child matches the
bottom band for the father ✓. So the alleged father could
be the child’s father ✓, though we cannot be 100% certain Student B
of that because there could be another man who has this
(a) The breakdown of a large molecule by the addition of
band as well ✓.
water. ✓ In glycogen, hydrolysis breaks the glycosidic
bonds ✓ between glucose molecules.
This is a clear and thorough conclusion. Mark: 3/3
This is correct. Mark: 2/2
Question 8
Student B
(b) Adrenaline binds to a receptor molecule in the cell
Adrenaline is a hormone secreted by the adrenal
membrane ✓. This activates a second ✓ messenger called
glands. One of its effects is to cause the hydrolysis
cyclic AMP ✓, which binds to kinase ✓ proteins in the
of glycogen to glucose in liver cells. cytoplasm. This causes phosphate groups to be added to
(a) Explain what is meant by hydrolysis. (2 marks) enzymes, which activates them. The activation of these
(b) Complete the passage, which describes how enzymes results in the activation of many molecules of
adrenaline brings about the hydrolysis of glycogen phosphorylase ✓, which hydrolyse glycogen to
glycogen to glucose in a liver cell. glucose.
Adrenaline binds to a receptor molecule
in the cell ......................... . This activates All the words are correct. Mark: 5/5
a ......................... messenger called cyclic
......................... , which binds to .........................
proteins in the cytoplasm. This causes Student B
phosphate groups to be added to enzymes, (c) When one adrenaline molecule binds to the cell surface
which activates them. The activation of these membrane, it sets off an enzyme cascade. ✓ At each step,
enzymes results in the activation of many one enzyme causes the activation of many more enzymes,
molecules of glycogen ......................... , which so by the time you get to glycogen phosphorylase there
hydrolyse glycogen to glucose. (5 marks) are many of them activated. ✓ Moreover, one glycogen
phosphorylase molecule can cause the breakdown of many
(c) Explain how a single molecule of adrenaline can
glycogen molecules ✓, because enzymes are not destroyed
cause the production of a very large number of
in the reaction. Each glycogen molecule is a polymer of
glucose molecules. (3 marks) glucose, so breaking down just one glycogen molecule
(Total: 10 marks) produces a very large number of glucose molecules. ✓
This is a good answer. The student has thought of

Student A
several different reasons (enzyme cascade, the ability of
(a) The breakdown of a large molecule to a small one. ✓ one enzyme molecule to catalyse many reactions, and the
fact that one glycogen molecule contains many glucose
This is correct, but only one point has been made. molecules). Many students would think of just one reason and
Mark: 1/2 stop there, so this student has done very well. Mark: 3/3

Question 9

Student B
(a) When the action potential arrives at the presynaptic
(a) Describe how a nerve impulse crosses a knob ✓, it causes calcium ion channels to open ✓. Ca2+
cholinergic synapse. (9 marks) flood into the neurone ✓, down their concentration
(b) Outline the functions of a sensory neurone and gradient ✓. The knob contains many tiny vesicles full of the
neurotransmitter ✓ acetylcholine ✓. The calcium ions make
a motor neurone in a reflex arc. (6 marks)
these vesicles move to the presynaptic membrane and
(Total: 15 marks) fuse with it ✓, releasing the acetylcholine into the synaptic
cleft ✓.
This is the extended writing question. This cleft is very small, so it takes only a millisecond or two
for the acetylcholine to diffuse ✓ across it. On the other side
Student A of the cleft, there are receptor molecules in the postsynaptic
(a) In order for the action potential to cross the synaptic cleft, membrane, and the acetylcholine molecules fit perfectly into
it has to go through a process. these. ✓ This makes sodium ion channels in the postsynaptic
A nerve impulse arrives at a synapse in the form of an action membrane open ✓, so sodium ions flood in down their
potential. It causes sodium and calcium gates to open so concentration gradient ✓. This depolarises the membrane ✓
these ions ✓ go into the presynaptic membrane 7. The (gives it a positive charge inside), which sets up an action
calcium causes the vesicles in the presynaptic neurone to potential in the postsynaptic neurone.
fuse with the membrane ✓ and release acetycholine into
the synapse. This binds to the receptors on the postsynaptic This is a good answer. Although it is short, it is packed
membrane ✓ and this makes them pump 7 sodium ions into with correct and relevant detail. There are no mistakes,
the neurone which starts up another action potential in the and no important steps have been omitted. The extended
second neurone, because it depolarises it ✓. answer questions usually have many more marking points
than the total number of marks available, so even though this
Then the enzyme acetylcholinesterase causes the
answer has 13 ticks it can still only get the maximum mark.
acetylcholine to go back into the presynaptic neurone 7, so it
Mark: 9/9
returns to the resting potential again.
Student B
This answer is not clear, and the examiner cannot always (b) A sensory neurone has its cell body in the ganglion in the
be certain what the student means. For example, the dorsal root of a spinal nerve. It has a very long dendron
calcium ions go through the presynaptic membrane and into that carries action potentials from a receptor towards its
the neurone, not ‘into’ the membrane. Notice that no mark is cell body ✓, and a shorter axon that carries the action
given for the mention of calcium until the term ‘ions’ is used potentials into the spinal cord (or brain) ✓. The ending of
later on. There is an error in the next to last sentence; the the dendron may be within a specialised receptor such
sodium ions are not pumped into the postsynaptic neurone, as a Pacinian corpuscle in the skin. ✓ Pressure acting on
but pass through the opened sodium ion channels by the Pacinian corpuscle depolarises the membrane of the
diffusion, down their concentration gradient. The last sentence dendron and generates an action potential. ✓ (In general,
also contains an error, as acetycholinesterase breaks down receptors transfer energy from a stimulus into energy in an
acetylcholine. In any case, this process is not strictly relevant to action potential.)
a description of how the impulse crosses the synapse, because
The motor neurone has its cell body within the central
it takes place after that event has finished. Overall, this is not a
nervous system (in the brain or the spinal cord). It has many
strong answer, with lack of detail and several errors. Mark: 4/9
short dendrites and a long axon. It will have many synapses,
Student A including several with sensory neurones. ✓ Thus the action
potential from a sensory neurone can cross the synapse and
(b) The sensory neurone transmits an action potential from
set up an action potential in the motor neurone ✓, which will
a receptor into the spinal cord. ✓ The action potential
then transmit it to an effector such as a muscle or gland ✓.
crosses a synapse and then passes along a motor neurone
The action potential then causes the effector to respond, for
to an effector, such as muscle. ✓ This makes the muscle
example by contracting (if it is a muscle). ✓
contract. ✓ This is a reflex action. The muscle automatically
contracts without you having to think about it. In a reflex arc, the impulses travel directly from the sensory
to the motor neurone (or sometimes via an intermediate
neurone between them) without having to be processed
There are three correct statements here, but a good
in the brain. ✓ This means the pathway from receptor to
IGCSE student could have given this answer and there is
effector is as short as possible, so the response can happen
not sufficient detail for a high mark at A level. Mark: 3/6
very quickly.
This is a good answer, taking each neurone in turn and

concentrating on functions, with only brief references to
structure where these are directly relevant to function. Mark:
6/6

Now test yourself 3 First do the reducing sugar test. Use excess Benedict’s
Now test yourself answers
solution so that all the reducing sugar is used up in the test.
Filter to remove the brick red precipitate. Collect the filtrate.
answers It would be a good idea to do the reducing sugar test again,

just to check that you really have got rid of all the reducing
sugar. If you have, then do the non-reducing sugar test.
4 a Secondary structure is the first-level coiling or folding
Topic 1 of the polypeptide chain. This is often an alpha helix
or a beta pleated strand. Tertiary structure is the
1 79 mm = 79 000 µm three-dimensional folding of the polypeptide chain, for
size of image example to form a globular shape.
magnification b A collagen molecule is a chain of amino acids linked
79 000 by peptide bonds. A collagen fibre is made up of many
=
16 000 collagen molecules linked together by covalent bonds
= 4.9 µm between lysine molecules.
2 100 graticule units = 8 × 0.01 mm 5 a A hydrogen bond is a weak attraction between a small
= 0.08 mm negative charge (e.g. on the oxygen of an –OH group)
0.08 and a small positive charge (e.g. on the hydrogen of an
So 1 graticule unit = –OH group).
100
= 0.0008 mm b Hydrogen bonds cause forces of attraction between
= 0.0008 × 1000 µm water molecules. This means that extra energy has to
be applied to separate the molecules from one another.
= 0.8 µm This means that the melting point and boiling point are
Cell measures 94 graticule units higher than for similar compounds that do not have
94 hydrogen bonding between their molecules.
= = 118 µm
0.8 c Carbohydrates, proteins
3 From scale bar with the virus diagram, 5 mm represents
1 nm
Diameter of virus in diagram = 40 mm
Topic 3
40 1 Variable to change (independent variable): catalase from
So actual diameter of virus = = 8 nm
5 different fruits.
From scale bar with the bacterium diagram,
Variable to measure (dependent variable): initial rate of
12 mm = 0.1 µm = 1 × 105 nm
reaction (e.g. volume of oxygen given off per second)
Length of bacterium in diagram = 54 mm
Variables to control: volume and concentration of catalase
54
So actual length = × 105 = 4.5 × 105 nm extract; volume and concentration of substrate (hydrogen
12
peroxide solution); temperature; pH
4.5 × 105
Therefore = 5.6 × 104 viruses could line up inside it. l Use a liquidiser to mash up the same mass of two fruits.
8
Run the liquidiser at the same speed for the same period
of time.
Topic 2 l Filter the extracts and collect equal volumes of each.
l Measure equal volumes of the extract into several test

1 Fructose: monosaccharide; gives positive result with
tubes.
Benedict’s test
l Use serial dilution to make up a range of concentrations
Glucose: monosaccharide; gives positive result with of hydrogen peroxide (see p. 78 for how to do this).
Benedict’s test l Add a small amount of pH 7 buffer to each tube.
Lactose and maltose: disaccharides; lactose is formed from l Place all tubes (fruit extract and substrate) in a water
glucose and galactose, maltose is formed from glucose and bath at 37 °C and leave for 20 minutes to come to
glucose; give positive result with Benedict’s test temperature.
Sucrose: disaccharide; formed from glucose and fructose; l Add fruit extract to one of the hydrogen peroxide
does not give positive result with Benedict’s test tubes, and measure the rate of oxygen production (e.g.
by collecting over water, or by placing on a balance and
2 Cellulose is made up long chains of glucose linked by β
measuring loss of mass).
1–4 glycosidic bonds. This results in the glucose molecules
l Repeat for each hydrogen peroxide concentration.
being alternately one way up, then the other. This means
l Plot graphs of oxygen production against substrate
that chains lying side by side can form hydrogen bonds with
concentration for each tube.
each other, making microfibrils. Starch, on the other hand, is
l Use your graphs to calculate the initial rate of reaction
made up of long chains of glucose linked by α 1–4 glycosidic
for each tube (see p. 28 for how to do this).
bonds. It twists into a spiral, and one starch molecule
l Plot graphs of initial rate of reaction against substrate
does not form bonds with others, so no fibrils are formed.
concentration for each of the two fruit juice extracts.
Moreover, it is difficult to break down β 1–4 glycosidic
l Use your graphs to find Vmax for each fruit juice extract.
bonds, so not many organisms can digest cell walls.

Read off the substrate concentration that corresponds to loading of sucrose into the phloem at a source, which causes

l
½Vmax . This gives you the value of Km. water to follow by osmosis. Different parts of the plant can
l The fruit juice extract with the larger value of Km is the
act as sources at different times — for example leaves when
one with greater affinity for its substrate. they photosynthesise or roots when the starch stored in
2 a Temperature, enzyme concentration, substrate them is broken down. Different parts can also act as sinks at
concentration, competitive inhibitors different times — for example roots in autumn when they
b Temperature, pH and non-competitive inhibitors are building up starch stores or flowers when they are using
sugars to produce nectar (so the flow can be upwards or
3 Enzymes do not contaminate the final product; enzymes are
downwards).
more stable so can work at a wider range of temperatures
and pH; enzymes are not lost, so can be reused many times. 4 a Leaves are sources; flowers and roots are sinks.
b Leaves are sources; fruits and roots are sinks.
Topic 4 c Roots are sources; leaves are sinks.
d Roots are sources; leaves and flowers are sinks.
1 Diffusion takes place when molecules or ions can move
freely through the membrane, through the phospholipid
bilayer. Facilitated diffusion takes place when molecules
Topic 8
or ions can only move through protein channels in the
1 No. The pulmonary artery carries deoxygenated blood.
membrane, not through the phospholipid bilayer. Note
that both processes are passive, and movement is down a 2 Time taken for one complete heart beat is about 0.75
concentration gradient. seconds. So in one minute there will be 60/0.75 = 80 beats.
2 Facilitated diffusion takes place down a concentration
gradient, and is passive. Active transport takes place up a Topic 10
concentration gradient and requires energy input from the
cell. Note that in both of these processes, molecules or ions 1 The cause of an infectious disease is the pathogen that
move through proteins in the membrane. enters the body and makes you ill. A vector for a disease is
an organism that transmits the pathogen into the body.
Topic 6 2 HIV-positive means that the person has been infected with
the virus. AIDS means that the virus is causing symptoms of
1 There is only room in the DNA double helix for one disease.
nucleotide with one ring, and one with two rings, to link
together. Moreover, C and G join with three hydrogen Topic 11
bonds, while A and T join with two.
2 Three 1 The mitochondria provide ATP for protein synthesis and
3 GUA, CCU, GAC secretion. The rough endoplasmic reticulum provides sites
for the synthesis of antibodies (which are proteins). The
Golgi bodies prepare the antibodies for secretion.
Topic 7
1 Your plan diagram should: Topic 12
l be large — preferably larger than the diagram in the
book 1 Phosphorylation raises the energy level of the molecule,

l be drawn with clear, clean lines
making it able to take part in the reaction.
l not show any individual cells 2 One proton and one electron
l be made up of four concentric lines — a pair of lines 3 If there is no soda lime then no carbon dioxide will be
quite close together on the outside to represent the absorbed. If the organisms are respiring aerobically using
epidermis and root hairs; a line much closer to the centre glucose as a substrate, then the volume of oxygen taken in
of your drawing representing the boundary between the will equal the volume of carbon dioxide given out, and there
cortex and the endodermis; another line close to the last will be no change in the level of fluid in the manometers.
one representing the inner edge of the endodermis; and
then a cross-shaped structure representing the xylem
and phloem. Topic 13
2 a Plant has to provide energy.
1 The absorption spectrum shows the wavelengths of light
b Plant does not have to provide energy. that can be absorbed. The action spectrum shows which
3 Movement of water up the xylem is down a water potential wavelengths of light can be used. We would therefore
gradient, from roots to leaves. This gradient is maintained by expect the two to be very similar to one another.
transpiration in the leaves, which lowers the water potential 2 The energy ends up in ATP molecules.
there. The gradient is always in the same direction.
3 Substrate: carbon dioxide
Movement of sap in phloem is down a pressure gradient,
Product: intermediate compound or GP
from source to sink. High pressure is produced by active
4 From the light dependent reaction.
Now test yourself answers 203

Topic 14 6 A plant with genotype LeLe already makes gibberellin, so
can already grow tall. Adding more gibberellin is unlikely to
1 No matter what the environmental temperature, enzymes have any effect.
will always be able to work at close to their optimum
temperature. This means that metabolism can continue at Topic 16
a fairly steady rate during day and night, and in different
seasons, and in different climatic regions of the world. This 1 The gametes in the self-pollinated plant will both be made
means that animals can be active at all of these times and in from mother cells of the same genotype. These gametes
different places. will vary slightly in their genotypes because of independent
2 One signalling molecule (e.g. a hormone molecule) activates assortment and crossing over, but this can only arise from
large numbers of glycogen phosphorylase enzymes. reshuffling of the same set of alleles in each. The gametes in
3 The biosensor gives a digital readout, which is easy to the cross-pollinated plant will have come from mother cells
interpret and has a continuous range. Dip sticks give a of different genotypes, in different plants. They will therefore
colour that you have to match against a colour scale, and have a different range of alleles. This will result in a wider
have a discontinuous range. range of possible allele combinations in the offspring from
the cross-pollinated plant.
4 The loss of water from the filtrate increases the
concentration of all the solutes in it. 2 Parents’ phenotypes Blood group AB × Blood group AB
5 There would be a water potential gradient from the Parents’ genotypes IAIB IAIB
cytoplasm of the red blood cell into the plasma. Water Gametes’ genotypes IA IB IA IB
would move out of the cell by osmosis, through its partially Offspring genotypes and phenotypes
permeable cell surface membrane. This would decrease the
volume of the cell, so it would shrink. IA IB
IAIA IAIB
Topic 15 IA Blood group A Blood group AB
1 The opening and closing of the potassium ion channels is IAIB IBIB
caused by changes in the potential difference across the IB Blood group AB Blood group B
membrane. They open when this is positive inside, and close
We would therefore expect to get blood group A, B and AB
when it is negative inside. They are said to be voltage-gated
in the ratio 1:1:2.
channels.
3 The allele for colour blindness is carried on the X
2 The vesicles containing transmitter substance are present
chromosome. A man passes only his Y chromosome to his
only in the presynaptic neurone, so the impulses can only
son.
be transmitted from this one, not from the postsynaptic
neurone. Also, there are only receptors for the transmitter 4 Parents’ phenotypes
substance on the postsynaptic neurone, not on the brown hair, long legs × black hair, short legs
presynaptic neurone. Parents’ genotypes AaLl aall
3 A muscle fibre is made up of many myofibrils arranged Gametes’ genotypes
side by side. It is a specialised cell. It is surrounded by a
AL Al aL al al
cell surface membrane (sarcolemma) and contains several
nuclei and other organelles, especially mitochondria Offspring genotypes and phenotypes
and endoplasmic reticulum. A myofibril is one of several AL Al aL al
cylindrical structures within a muscle fibre, and contains
filaments. A filament can be one of two types — either al AaLl Aall aaLl aall
actin or myosin — and is essentially a protein molecule. brown, brown, black, black,
4 The mitochondria are the sites in which the Krebs cycle and long short long short
the electron transport chain generate ATP. ATP is required
for the detachment of the myosin heads from the actin
filaments, allowing the filaments to slide past one another
and cause the muscle to contract.
5 Your table could include the following:
l Both are small molecules that act as cell signalling first
messengers.
l Animal hormones are carried in the blood, but plant
hormones travel in phloem or by diffusion.

l Animal hormones are made in endocrine glands,
but plant hormones are made in tissues that are not

organised into glands.

5 Parents’ phenotypes b If the genes are not linked:

brown eyes, dark fur × brown eyes, dark fur Parents’ phenotypes
Parents’ genotypes yellow, large × yellow, large
EeFf EeFf Parents’ genotypes
Gametes’ genotypes YyPp YyPp
EF Ef eF ef EF Ef eF ef Gametes’ genotypes
Offspring genotypes and phenotypes YP Yp yP yp YP Yp yP yp
EF Ef eF ef Offspring genotypes and phenotypes
YP Yp yP yp
EF EEFF EEFf EeFF EeFf
brown, brown, brown, brown,
YP YYPP YYPp YyPP YyPp
dark dark dark dark yellow, yellow, yellow, yellow,
Ef EEFf EEff EeFf Eeff large large large large
brown, brown, brown, brown, YYPp YYpp YyPp Yypp
Yp
dark pale dark pale yellow, yellow, yellow, yellow,
eF EeFF EeFf eeFF eeFf large small large small
brown, brown, blue, blue, YyPP YyPp yyPP yyPp
yP
dark dark dark dark yellow, yellow, white, white,
ef EeFf Eeff eeFf eeff large large large large
brown, brown, blue, blue, YyPp Yypp yyPp yypp
yp
dark pale dark pale yellow, yellow, white, white,
The shaded rows and columns show the uncommon large small large small
gametes and offspring phenotypes. We would therefore This would give a ratio of 9 yellow, large:3 yellow, small:
expect most offspring to be in the ratio 3 brown, dark:1 blue, 3 white, large:1 white, small
pale. There will be a small number of other combinations. 7 First, work out the expected numbers if there is no linkage.
6 a If the genes are linked: You should find that you would expect a 1:1:1:1 ratio of
Parents’ phenotypes yellow, large × yellow, large phenotypes. (See answer to Question 4 above.)
Parents’ genotypes YyPp YyPp White, White, Grey, Grey,
Gametes’ genotypes YP yp YP yp long short long short
Offspring genotypes and phenotypes Observed 12 2 2 12
numbers, O
YP yp Expected 7 7 7 7
numbers, E
YYPP YyPp
YP yellow, large yellow, large O−E 5 −5 −5 5
(O − E)2 25 25 25 25
YyPp yypp
yp yellow, large white, small (O − E)2 3.6 3.6 3.6 3.6
E
This would give a ratio of 3 yellow, large:1 white, small 2
Σ (O − E) = 14.4
if the genes are totally linked. If there is some crossing E
over, we would expect to get small numbers of other
phenotypes. We have four different categories, so the number of degrees
of freedom is 3. Looking up the value of chi-squared for 3
degrees of freedom, we find that our number lies between
a probability of 0.01 and 0.001 that the null hypothesis (i.e.
there is no linkage) is correct. This is much less than the
critical value of 0.05, so we can say that the null hypothesis
is not correct, and that our results show that the two genes
are linked.

8 Parents’ phenotypes agouti × agouti
Species n n/ N (n/N)2
Parents’ genotypes AaBB AaBb A 2 0.010 0.000
Gametes’ genotypes AB aB AB Ab aB ab B 35 0.174 0.030
Offspring genotypes and phenotypes C 1 0.005 0.000
AB aB D 81 0.403 0.162
E 63 0.313 0.098
AB AABB AaBB
agouti agouti F 2 0.010 0.000
G 5 0.025 0.001
Ab AABb AaBb
agouti agouti H 11 0.055 0.003
AaBB aaBB I 1 0.005 0.000
aB
agouti black
ab AaBb aaBb Σ (Nn ) = 0.294
2
agouti black So D = 1 − 0.294 = 0.706

We would expect offspring in the ratio 3 agouti : 1 black.
9 A white mouse could have the genotype AAbb, Aabb or Topic 19
aabb. We could cross this mouse with a pure-breeding black
mouse, with the genotype aaBB. 1 Selective breeding can only reshuffle alleles that are already
If the unknown mouse has the genotype AAbb, then all the present in a species. Gene technology introduces completely
offspring will be agouti (AaBb). new alleles from a different species.
If the unknown mouse has the genotype Aabb, then half the 2 Gene therapy can only add genes, not remove them. If a
offspring will be agouti (AaBb) and half will be black (aaBb). condition is caused by a dominant allele, this will still be in
If the unknown mouse has the genotype aabb, then all the the cell, so even if a ‘correct’ recessive allele is introduced, it
offspring will be black (aaBb). will not have any effect.
10 It is wasteful to produce the enzyme when there is no
substrate for it. The bacterium saves energy and materials by A level experimental skills and
not making the enzyme in these circumstances.
investigations
Topic 17 1 x
−
(x − X )
−
(x − X )2
23.0 −17.5 306.25
1 The graph should have two peaks, like a two-humped
46.5 6.0 36.00
camel.
52.0 11.5 132.25
2 Frequency of the homozygous recessive animals (q2) is 100
in 5000, or 0.02. 32.5 −8.0 64.00
a So q is the square root of 0.02, which is 0.14. 43.0 2.5 6.25
b p is 1 − 0.14, which is 0.86. 50.5 10.0 100.00
So the frequency of heterozygotes is 2pq, which is 2 × 26.5 −14.0 196.00
0.14 × 0.86 = 0.24. 31.5 −9.0 81.00
We would therefore expect about 24% of the animals to 54.0 13.5 182.25
be heterozygotes. 28.0 −12.5 156.25
3 Courtship behaviour and sperm survival are pre-zygotic. 47.5 7.0 49.00
Chromosome number and inability to form gametes are
39.0 −1.5 2.25
post-zygotic.
58.5 18.0 324.00
47.5 7.0 49.00
Topic 18 33.5 −7.0 49.00
38 × 40 42.5 2.0 4.00
1 Estimated total population size =
6
26.5 −14.0 196.00
= 253
51.0 10.5 110.25
2 Calculate n/N for each species. Then square each value. Add
38.0 −2.5 6.25
them up and subtract from 1.
38.5 −2.0 4.00
− −
mean X 40.5 Σ(x − X )2 = 2051.75
2051.75
So s = = 10.4
√ 19

s 4

2 SM =
√n
= 10.4/4.47 Shell length/ Body mass/
= 2.3 mm Mollusc mm (x) g (y) xy
3 1 38 6.1 231.8
2 18 3.6 64.8
Number Number Rank for 3 20 3.2 64.0
of species Rank for of species
Quadrat P species P species Q Q 4 31 5.7 176.7
1 6 6 5 7 5 12 2.6 31.2
2 12 2= 22 2 6 12 3.2 38.4
3 1 9 3 9= 7 25 4.7 117.5
4 0 10 4 8 8 20 3.6 72.0
5 16 1 35 1 9 29 4.7 136.3
6 8 5 10 5 10 19 3.3 62.7
7 11 4 11 4 mean
8 4 8 9 6 x = 22.4
9 5 7 3 9= y =4.07
10 12 2= 21 3 Σ xy = 995.40
Rank for
nxy = 10 × 22.4 × 4.07 = 911.68
Rank for species Difference standard deviation for x = 8.34
Quadrat species P Q in rank, D D2 standard deviation for y = 1.17
1 6 7 −1 1 Σ xy – nxy
r =
2 2= 2 0 0 nsx sy
3 9 9= 0 0
So:
995.40 − 911.68
4 10 8 2 4 r=
97.578
5 1 1 0 0 = 0.86
6 5 5 0 0 This number is close to 1, suggesting that there is a linear
7 4 4 0 0 correlation between these two sets of data.
8 8 6 2 4
9 7 9= −2 4
10 2= 3 −1 1
Σ D2 = 14
Substitute into the equation:

6 × 14
rs = 1 − 3
10 − 10
84
=1−
990
= 1 − 0.08
= 0.92
This is well above the critical value for rs when n = 10, so
there is a significant correlation between the distributions of
P and Q.

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International AS and A Level

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© Mary Jones 2015

First printed 2015

Cover photo reproduced by permission of Fotolia

Typeset by Greenhill Wood Studios, UK

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off each section as you revise. 1 Cell structure

specimen has to be very

Features to help you succeed 4

Expert tips Exam-style questions

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4 Cambridge International AS and A Level Biology Revision Guide

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180 How to make the most of your practical skills . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ■ ■ . . . . . . . . . . . . . . . . . ■

202 Now test yourself answers

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6 Cambridge International AS and A Level Biology Revision Guide

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Magnification and resolution

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l As it is a very small object, convert this measurement to µm by multiplying by 1000:

l Calculate its magnification using the formula

size of image Expert tip

l Calculate its real length using the formula

Now test yourself Tested

8 Cambridge International AS and A Level Biology Revision Guide

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An eyepiece graticule is a little scale bar that you can place

lined up with the 1.0 mark on the eyepiece graticule. Work

Now test yourself Tested

2 A cell measures 84 small eyepiece graticule units.

Hodder CIE Biology.indd 9 21/10/2015 17:20

Lysosomes Golgi body Nucleus Nucleolus Cell surface Rough endoplasmic

Mitochondrion Centrioles 80S 70S Smooth endoplasmic

Rough endoplasmic Nucleus Nucleolus Smooth endoplasmic

Plasmodesma Mitochondrion Golgi body Nuclear envelope

Functions of membrane systems and organelles Revised

10 Cambridge International AS and A Level Biology Revision Guide

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1 Proteins are made

Outer membrane Inner membrane Intermembranal

70S ribosomes Small circular DNA 0.1 µm

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are surrounded by an envelope made up of two membranes (Figure 1.9).

70S ribosomes Stroma Outer membrane

Prokaryotic cells Revised

Cell wall made of Cytoplasm

0.1 µm 70S ribosomes

12 Cambridge International AS and A Level Biology Revision Guide

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