Ans PWB E4 e
Ans PWB E4 e
Ans PWB E4 e
fragments
Understanding procedure (p. 104)
1 Free nucleotides act as the building blocks of the newly synthesized DNA strands.
2 Heat-stable DNA polymerase is not easily denatured by heat. It can catalyse the synthesis
of new DNA strands at high temperature.
3 To collect all the reaction mixture from the inner wall of the PCR tubes.
2
PCR tube DNA band
A +
B +
C -
2 Inaccurate volumes of reagents are transferred due to pipetting errors. / Some reagents
remain on the inner wall of the PCR tubes before PCR.
3 Prewet the micropipette tip with the reagent before use. / Centrifuge the reaction mixture
for a longer period of time to collect all the reagents from the inner wall of the PCR
tubes before PCR.