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Chapter- 7

Conclusion
Chapter 7 Conclusion

CONCLUSION

Mango is the 'King of fruits', fifth largest and important fruit of tropical

world belongs to the family Anacardiaceae. India produces 70% of the world's

Mangoes. The soil and climatic conditions of India are highly suitable for Mango

cultivation. The fruit is large, fleshy, drupe containing a laterally compressed stone,

housing the seed. Mango cultivars vary considerably in fruit size, colour, shape,

flavour, texture and taste.

India is the home of about 1,000 varieties of Mango. Most of them are the

result of open pollination arisen as chance seedlings. However, only a few varieties

are commercially cultivated throughout India.

Bacteria seem to be the most primitive living organisms, diagnosis of a

bacterial disease and identification of the causal bacterium is based on the symptoms

of the disease, the constant presence of large numbers of bacteria in the affected

area.

Bacterial black spot diseases of mango casual organism is Xanthomonas

campestris pv. mangiferaeindicae, the mango leaves, stems and fruits are all

susceptible to infection, on leaves it produce angular, water soaked spots, which

delimited by the veins. Stem lesions appear as blackened cankers that form

longitudinal cracks with bacterial exude. Fruit drop occurs especially when infection

start on young fruits or when stalks become fruit infected. The disease attacks

through natural openings such as stomata, wax and oil glands, leaf and fruit

abrasions, leaf scars and at the apex of branches in the panicle, in young trees the

diseases can cause dieback of branches.

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Chapter 7 Conclusion

Plants with the disease symptoms were collected randomly from major

mango production districts in Kamataka are Kolar, Tumkur, Chitradurga, Belgaum,

Dharwad, Koppala, Chikkamagalur, Chikkaballapura, Mysore and Bangalore Rural,

fields parts such as leaves, fruits and stem, brought to the laboratory and screened

for the target pathogen Xanthomonas campestris pv. mangiferaeindicae by direct

plating, liquid assay methods and identified the isolates on the basis of

morphological, cultural, biochemical and pathogenicity tests as per the standard

microbiological procedures and results were compared with the authentic culture.

Genetic variation was identified by the Polymerase Chain Reaction-Random

Amplified Polymorphic DNA (PCR-RAPD), Xanthomonas campestris pv.

mangiferaeindicae isolates selected from different district and regions of Kamataka,

The RAPD analysis Band statistics and cultivar similarity of RAPD markers further

confirmed polymorphism among the studied 15 Xcm accessions

Population analysis dendrogram generated based on the UPGMA analysis

from the 15 bacterial genotypes data clearly grouped into two broad clusters at

80.6% dissimilarity. The group 1 contains 8 strains (KUM, DAS, PAV, GOB, PER,

BET, MUT, RAYL) and remaining group contains 7 strains (MLK, TVG, BELG,

YAR, KRC, BIS, LIN) formed a cluster. The PER and BET strains shared very low

variability and formed cluster at 30.6% dissimilarity.

In order to control the growth of Xanthomonas campestris pv.

mangiferaeindicae pathogen in vitro conditions by Antagonistic organisms.

Antibiotics, commercial formulates and macro fungus and plant extracts using

different solvents like pet ether, chloroform, methanol and water.

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Chapter 7 Conclusion

Among all the control methods, commercial formulates are very effective to

control the Xanthomonas campestris pv. mangiferaeindicae followed by the other

control methods.

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