Proteins and Amino Acids I: Color Reaction: Chemlab204: General and Inorganic Chemistry

ChemLab204: General and Inorganic Chemistry Group No.
62
Laboratory Report Date Submitted: 01-12-16
Date
Proteins and Amino

Acids I: Color reaction
Activity No. 11
Prince Mark B. Badillo

ID No. 150055; BSN-I
ChemLab204A; 2nd Semester, SY 2015-16
RATIONALE
________________________________________________________________________________________________________
Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga
University
In this activity, three classes of organic compounds will be examined: esters,
aldehydes and ketones. The objective of this activity is to utilize the different test
(Test for Carbonyl group, Test for Aldehydes, Test for Methyl Ketones, Special Test for
Benzaldehyde and Molisch Test) to differentiate unknown substance based on the
characteristic of Esters, Aldehydes and Ketones. To observe properly the different
indications of each test and classify whether one is an aldehyde ketone or ester. To
know the roles of the reagents used in the different test, and lastly to interpret the
different test results with accuracy.
The purpose of this activity. It has a significance when it comes to the field of
medicine or nursing. It is known that sodium nitroprusside is not just a solution used
to be tested in different laboratory experiments. Sodium Nitroprusside is known to
detect cysteine in the urine, cysteines are known to be crystals in our kidney. This
crystals can be able to form kidney stone. The role of sodium nitroprusside is to
detect cysteine to prevent kidney stones to happen. The first reaction yields to ethyl
acetate
INTERPRETATION:
CHEMICAL EQUATION:
A. Biuret Reaction
Name of Test Test Solutions Reagents Added Result
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University
A. Biuret 10% NaOH
1% Albumin
Reaction 1% CuSO4
1% Casein
1% Glycine
1% Peptone
Urea (s)
INTERPRETATION:
CHEMICAL EQUATION:
B. Millon’s Test
Millon’s Reagent [HgNO3

B. Millon’s Test 1% Albumin and Hg(NO3)2 added
with conc. HNO3]
1% Casein
1% Gelatin
1% phenol
INTERPRETATION:
CHEMICAL EQUATION:
________________________________________________________________________________________________________
University
C. Xanthoproteic Reaction
C. conc. HNO3
Xanthoproteic 1% Albumin conc. NH4OH
Reaction
1% Casein
1% Gelatin
1% Phenol
INTERPRETATION:
CHEMICAL EQUATION:
D. Test for Sulfur
A. Test For 10% NaOH

1% Albumin
Sulfur 5% Pb(CH3COO)2
Gelatin (s)
Peptone (s)
INTERPRETATION:
CHEMICAL EQUATION:
________________________________________________________________________________________________________
University
E. Hopkins- Cole Raction
E. Hopkins- conc. H2SO4

1% Albumin
Cole Reaction
1% Casein
1% Gelatin
INTERPRETATION:
CHEMICAL EQUATION:
F. Ninhydrin Reaction
F. Ninhydrin 0.1% ninhydrin solution

0.2% Albumin
Reaction
Ammonia water
0.2% Urea
0.2% Glycine
ANSWERS TO QUESTIONS
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University
1. Why do all proteins give a positive result for Biuret test?
2. Why does nitric acid stain the skin with a yellow color?
3. Will methionine give a positive result for the sulfur test? Explain.
4. What grouping in amino acids or proteins is responsible for the ninhydrin

reaction?
5. What color will be produced by proline with ninhydrin? Why?
CONCLUSION
Different tests were done to several known substance for it to be able to

classify as esters, aldehydes or ketones. In doing the different test, exerting a lot of
effort and accuracy was done in order to obtain a correct data. In the different
laboratory tests, some indicates a positive result and some did not, this is due to
the purpose of the test which is to classify whether a given substance will indicate a
positive result or negative result when it is being tested.
REFERENCES
1. Laboratory manual ( 2nd semester) Activity 7 “Esters, Aldehydes and

ketones”, Natural Sciences and Department, CSIT, ADZU
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University
2. Fuson, R., & Shriner, R. (1964). The systematic identification of organic
compounds. New York: John wiley & sons, inc.
3. Bettelheim, Landesberg. “Laboratory experiments for general, organic &
biochemistry.”
4. Morisson and boyd. Title: organic chemistry, 4 th ed
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University

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ChemLab204: General and Inorganic Chemistry Group No.

Laboratory Report Date Submitted: 01-12-16

Proteins and Amino

Prince Mark B. Badillo

Name of Test Test Solutions Reagents Added Result

Name of Test Test Solutions Reagents Added Result

Millon’s Reagent [HgNO3

Name of Test Test Solutions Reagents Added Result

D. Test for Sulfur

Name of Test Test Solutions Reagents Added Result

A. Test For 10% NaOH

Name of Test Test Solutions Reagents Added Result

E. Hopkins- conc. H2SO4

Name of Test Test Solutions Reagents Added Result

F. Ninhydrin 0.1% ninhydrin solution

4. What grouping in amino acids or proteins is responsible for the ninhydrin

5. What color will be produced by proline with ninhydrin? Why?

Different tests were done to several known substance for it to be able to

1. Laboratory manual ( 2nd semester) Activity 7 “Esters, Aldehydes and

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