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GATE XL 2013 Exam Microbiology Solved Question Paper PDF

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Examination

(Original Question Paper with Answer Key)


GRADUATE APTITUDE TEST IN ENGINEERING

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2013 Life Sciences– XL

GATE XL 2013 K:MICROBIOLOGY

Q. 1 – Q. 10 carry one mark each.

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Q.1 In 1976, Tonegawa’s experiment gave clue about gene rearrangement during differentiation of B-
cells. The two different types of cells used in this experiment were
(A) HeLa cells and fibrosarcoma cells
(B) embryonic cells and fibroblasts
(C) adult myeloma cells and HeLa cells D
(D) embryonic cells and adult myeloma cells

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Q.2 To which one of the following groups, the antibiotics kanamycin, streptomycin and gentamicin
belong

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(A) cephalosporins (B) macrolides (C) aminoglycosides (D) quinolones
C
Q.3 Shine Dalgarno’s sequence present in mRNA binds to

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(A) 3 end of rRNA
(B) 5 end of rRNA
(C) 5 end of tRNA
A
(D) 3 end of tRNA

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Q.4 Which one of the following transport mechanism is NOT employed by prokaryotes?
(A) Passive diffusion (B) Group translocation (C) Endocytosis (D) Active transport C
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Q.5 The most common indicator organism of faecal pollution in water is
(A) Clostridium botulinum (B) Bacillus subtilis (C) Escherichia coli (D) Clostridium tetani C
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Q.6 The theoretical maximum number of ATP molecules produced from aerobic oxidation of glucose
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by eukaryotic cells is
(A) 38 (B) 24 (C)12 (D) 8
A
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Q.7 Which one of the following DO NOT use water as an electron source during photosynthesis?
(A) Sulfate reducing bacteria
(B) Methanogenic bacteria
(C) Green and purple bacteria
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(D) Nitrifying bacteria C

Q.8 surface area


If the radius of a spherical coccus is 0.8 μm, the value of in μm-1 will be
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volume
(A) 7.45 (B) 4.05 (C) 3.75 (D) 0.85 C
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Q.9 The enzyme that catalyzes the reduction of nitrogen to ammonia is


(A) nitrogenase (B) nitrate reductase (C) nitrite reductase (D)deaminase A
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Q.10 Chemostat is a continuous culture system in which sterile medium is fed into the culture vessel at
the same rate as the spent medium is removed. If in a chemostat culture, the flow rate is 30 ml h-1
and volume of the medium inside the vessel is 100 ml, the dilution rate in h-1 is
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(A) 3.33 (B) 1.50 (C) 0.75 (D) 0.30

XL 18/27
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2013 Life Sciences– XL

Q. 11 - Q. 20 carry two marks each.


Q.11 In an experiment the structural genes lacZYAof lac operon were found to be constitutively

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expressed. The following explanations were given for the constitutive expression

(P) absence of a functional repressor due to mutation in the repressor gene lacI
(Q) mutation in the operator that can no longer bind the repressor
(R) mutation in the lacAgene

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Which of the following is CORRECT
(A) Only P (B) Only Q (C) Both P & Q (D) Only R C

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Q.12 Which one of the following is TRUE about siderophores in bacteria?
(A) Siderophores are secreted only when soluble iron is available in the medium
(B) Siderophores form complex with ferrous ions in the medium
(C) Siderophores are the only route of iron uptake
D

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(D) Siderophores form complex with ferric ions in the medium

Q.13 In a population containing fast and slow growing bacteria, the slow growing bacteria can be

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enriched by supplementing the medium with
(A) chloramphenicol (B) penicillin (C) penicillin & chloramphenicol (D) rifampin B
Q.14 When the supply of tryptophan is plentiful, the tryptophan operon is repressed because the
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(A) repressor protein-corepressor complex is bound at the operator
(B) repressor protein is synthesized in large quantity
(C) repressor-corepressor complex is not formed
A
(D) repressor becomes inactive and therefore has reduced specificity for the operator
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Q.15 Match the scientists in Group I with their contributions in microbiology in Group II

Group I Group II
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P. Robert Hooke I. Proved that microbes can cause diseases


Q. Paul Ehrlich II. First to observe cells
R. Antony van Leeuwenhoek III. First to observe bacteria
S. Sergei Winogradsky IV. Used the first synthetic chemotherapeutic agent
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V. Linked specific bacteria to biogeochemical


transformations
(A) P-I, Q-II, R-III, S-V (B) P-II, Q-IV, R-III, S-V
B
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(C) P-II, Q-I, R-III, S-IV (D) P-V, Q-III, R-IV, S-II
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XL 19/27
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2013 Life Sciences– XL

Q.16 Match the infectious agents in Group I with the associated diseases in Group II

Group I Group II

P. Bordetellapertusis I. Mumps

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Q. Mycobacterium leprae II. Meningitis
R. Haemophilusinfluenzae III. Tuberculosis
S. Rubella IV. Whooping cough
V. Hansen’s disease
(A) P-IV, Q-V, R-II, S-I (B) P-V, Q-III, R-I, S-IV ABCD

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(C) P-I, Q-III, R-V, S-IV (D) P-III, Q-II, R-V, S-I

Q.17 Match the microscopes in Group I with their working principles in Group II

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Group I Group II

P. Phase contrast I. Light reaches the specimen only from the sides
Q. Dark field II. Uses fluorescent dyes

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R. Bright field III. Difference in the refractive index of cells from their
S. Electron microscopy surrounding medium
IV. Difference in contrast between specimen and its

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surrounding medium
V. Uses electrons instead of photons as energy source
(A ) P-V, Q-II, R-III, S-V (B)P-III, Q-I, R-IV, S-V
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(C) P-III, Q-II, R-V, S-I (D) P-II, Q-I, R-III, S-V
B

Q.18 In a phenol coefficient test for determining the efficacy of a disinfectant X, the maximum effective
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dilution for X and phenol was found to be 1/450 and 1/90, respectively. Calculate the phenol
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coefficient for X.
(A) 10.0 (B) 5.0 (C) 1.0 (D) 0.2
B
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Q.19 How many electrons are accepted when sulfate acts as the terminal electron acceptor in bacteria
such as Desulfovibrio?
(A) 8 (B) 6 (C) 4 (D) 2
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Q.20 A bacterial population increases from 103 cells to 109 cells in 10 h. Calculate the number of
generations per hour.
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(A) 20 (B)10 (C)4 (D)2


D
END OF SECTION - K
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XL 20/27
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