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Antimicrobial Effect of Plant Oils against Some Bacterias Izolated from

Patients Samples

Article  in  Journal of molecular and genetic medicine: an international journal of biomedical research · June 2018
DOI: 10.4172/1747-0862.1000318

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 ular and Ge

Journal of Molecular and Genetic

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Balkan et al., J Mol Genet Med 2018, 12:1
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DOI: 10.4172/1747-0862.1000318

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ISSN: 1747-0862

Research Article
Research Article Open Access
Open Access

Antimicrobial Effect of Plant Oils against Some Bacterias Izolated from

Patients Samples
Balkan CE1, Kordali S2 and Bozhüyük AU3
Faculty of Medical Science, Department of Medical Microbiology, Kafkas University, Turkey
1

Faculty of Agriculture, Department of Plant Protection, Ataturk University, Turkey

2

Faculty of Agriculture, Department of Plant Protection, Iğdır University, Turkey

3

Abstract
Aim: The aim of present study was to compare the antibacterial potential of Thymus sipyleus boiss. subsp.
sipyleus boiss. var. sipyleus L. (Thymol and camphor both tested), Satureja thymbra L. and Origanum onites L. by
Kirby Bauer disc diffusion method.
Methods: We isolated Enterococcus spp., E. coli, Morgonella morganii, Pseudomonas aeruginosa, MSSA,
Klebsiella pneumonia and Proteus mirabilis from different patient samples. The antibacterial activity of each oils
was evaluated by the Kirby Bauer disc diffusion method.
Results: Proteus mirabilis were the most susceptible bacteria against essential oils, followed by Klebsiella
pneumonia and Morganella morganii. Pseudomonas aeruginosa did not show susceptibility to any oil.
Conclusion: The essential oils of the T. sipyleus, O. onites and S. thymbra were especially very effective
against the resistant strains such as Enterococcus spp. and Klebsiella pneumonia. In this group the maximum
antimicrobial activity was observed with the essential oils of Origanum onites and Satureja thymbra. We think that
these oils can be proposed in treatment process as an alternative application structures in the future.

Keywords: Antimicrobial; Disc diffusion; Essential oils of Agriculture, the Department of Plant Protection, Erzurum, Turkey.
Aerial parts of the plants were dried in shade and ground in a grinder.
Introduction The dried plant samples (500 g) were subjected to hydro distillation
This study was undertaken to determine the in vitro antimicrobial for 4 h using a Clevenger-type apparatus. The oil yields of O. onites, S.
activities of three commercial essential oils and their main components thymbra and T. sipyleus were 4.5%, 2.3%, 1.17% and 0.98% (w/w, dry
in order to pre-select candidates for potential infections. The weight basis), respectively. The yield was based on dry materials of plant
antibacterial effects against pathogenic bacteria which are isolated samples. The oils were dried over a hydrous Na2SO4 and stored under
from patient’s samples (Enterococcus spp., E. coli, Morgonella morganii, N2 in a sealed vial until required, and then stored at 4°C until used for
Pseudomonas aeruginosa, MSSA, Klebsiella pneumonia and Proteus toxicity bioassays. The pure compounds were purchased commercially
mirabilis) were tested using paper disk diffusion method.   These from Fluka and Sigma. [Camphor (Fluka, purity 97%), Thymol (Sigma,
materials could be served as an important natural alternative to prevent purity 95%)]. The compounds tested for toxicity against Enterococcus
bacterial infections in the future (Table 1). spp., E. coli, Morgonella morganii, Pseudomonas aeruginosa, MSSA,
Klebsiella pneumonia and Proteus mirabilis.
One of the most popular spices used around the world is Origanum
onites L. It is used also as a medicinal therapy, arousing interest not Collection of bacteria
only in the use of its leaves in folk medicine, but also in relation to its
Enterococcus spp., E. coli, Morgonella morganii, Pseudomonas
essential oil for therapeutic purposes and treatment [1].
aeruginosa, MSSA, Klebsiella pneumonia and Proteus mirabilis isolated
Species of the genus Satureja (family Lamiaceae) are widely from different patient samples in the laboratory of clinical microbiology
distributed in the Mediterranean area, Asia, regularly found in warm, with VITEC microbial identification system.
dry, rocky habitats also in east part of Turkey [2]. Satureja consists
of about 200 species, usually aromatic herbs and used for treatment. Kirby-Bauer disc diffusion method
S. thymbra oil has been found to have a good antimicrobial activity The experiment was performed with a bacterial inoculum of 0.5
against various bacteria [3-5]. McFarland; Mueller-Hinton (MH) was inoculated with each bacterial
The genus Thymus, member of the Lamiaceae family, contains about
400 species of perennial aromatic, evergreen or semi-evergreen various
plants with many subspecies, varieties, sub-varieties and forms are mostly *Corresponding author: Dr. Çiğdem Eda Balkan, Faculty of Medical Science,
Department of Medical Microbiology, Kafkas University, Turkey, Tel: 90 474 225 11
distributed in the Mediterranean area and also in Turkey [6]. 67; E-mail: cigdemedabalkan@gmail.com

Materials and Methods Received December 28, 2017; Accepted January 12, 2018; Published January 16,
2018
Plant material and isolation of essential oil Citation: Balkan CE, Kordali S, Bozhüyük AU (2018) Antimicrobial Effect of Plant
Oils against Some Bacterias Izolated from Patients Samples. J Mol Genet Med 12:
Origanum onites L. (Lamiaceae), L. (Labiatae), Satureja thymbra
318 doi:10.4172/1747-0862.1000318
L. (Labiatae), Thymus sipyleus boiss. subsp. sipyleus boiss. var. sipyleus
L. (Lamiaceae) were collected at the flowering stage from different Copyright: © 2018 Balkan CE, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits
regions of Turkey between June and August. Voucher specimens unrestricted use, distribution, and reproduction in any medium, provided the
have been deposited in the herbarium of Ataturk University, Faculty original author and source are credited

J Mol Genet Med, an open access journal

ISSN: 1747-0862 Volume 12 • Issue 1 • 1000318
Citation: Balkan CE, Kordali S, Bozhüyük AU (2018) Antimicrobial Effect of Plant Oils against Some Bacterias Izolated from Patients Samples. J Mol
Genet Med 12: 318 doi:10.4172/1747-0862.1000318

Page 2 of 2

pathogen or non-pathogen bacteria cannot be attributed to one specific

mechanism [12].
Enterococcus spp., E. coli, Morgonella morganii, Pseudomonas
aeruginosa, MSSA, Klebsiella pneumonia and Proteus mirabilis are
the most important causes of some infections, and the emergence of
resistance to antibiotics is a serious public health problem.

Conclusion
In our hands, comparison of their results of this study with
previously published data is difficult because the composition of
plant oil products is known to vary according to local air conditions,
Figure 1: Muller-Hinton agar base with each bacteria and oils in a sterile filter
paper disc. After 24 hours period inhibition zones were measured.
soil composition and to extraction techniques. Moreover, the results
obtained may differ because of the method used to assess antimicrobial
activity and quantity of oil.
Entero- Morgan- Pseudomo- Klebsiella
Thymus E. Proteus
coccus ella mor- nas aerugi- MSSA pneumo- The essential oils of the T. sipyleus, O. onites and S. thymbra were
sypleus coli mirabilis
Spp. ganii nosa nia
especially very effective against the resistant strains such as Enterococcus
Kirby- 14 13
Bauer
17 mm
mm
20 mm 0 mm
mm
22 mm 23 mm spp. and Klebsiella pneumonia. The maximum antimicrobial activity
Satura- Entero- Morgan- Pseudomo- Klebsiella was observed with the essential oils of Origanum onites and Satureja
E. Proteus thymbra.
ja thym- coccus ella mor- nas aerugi- MSSA pneumo-
coli mirabilis
bra Spp ganii nosa nia
References
Kirby- 18 14
21 mm 30 mm 0 mm 18 mm 30 mm
Bauer mm mm 1. Sarac N, Ugur A (2008) Antimicrobial activities of the essential oils of Origanum
Origa- Entero- Morgan- Pseudomo- Klebsiella onites L, Origanum vulgare L. subspecies hirtum (Link) Ietswaart, Satureja
E. Proteus
num coccus ella mor- nas aerugi- MSSA pneumo- thymbra L, and Thymus cilicicus Boiss and Bal growing wild in Turkey. J Med
coli mirabilis
onites Spp ganii nosa nia Food 11: 568-573.
Kirby- 18
18 mm 16 mm 0 mm 9 mm 13 mm 18 mm 2. Giweli A, Džamić AM, Soković M, Ristić MS, Marin PD (2012) Antimicrobial
Bauer mm
and antioxidant activities of essential oils of Satureja thymbra growing wild in
Entero- Morgan- Pseudomo- Klebsiella Libya. Molecules 17: 4836-4850.
Cam- E. Proteus
coccus ella mor- nas aerugi- MSSA pneumo-
phor coli mirabilis
Spp ganii nosa nia 3. Gören AC, Topçu G, Bilsel G, Bilsel M, Wilkinson JM, et al. (2004) Analysis
Kirby- 0 of essential oil of Satureja thymbra by hydrodistillation, thermal desorber,
0 mm 0 mm 0 mm 0 mm 12 mm 0 mm
Bauer mm and headspace GC/MS techniques and its antimicrobial activity.  Nat Product
*Mean values in Kirby-Bauer disc diffusion method expressed as mm Res18: 189-195.

Table 1: The results of the antibacterial assays in terms of Kirby Bauer (Inhibition 4. Sarac N, Ugur A (2008) Antimicrobial activities of the essential oils of Origanum
ranged from 0 mm to 30 mm). onites L, Origanum vulgare L. subspecies hirtum (Link) Ietswaart, Satureja
thymbra L and Thymus cilicicus Boiss and Bal growing wild in Turkey. J Med
strain. Each oil was applied to a sterile filter paper disc (6-mm diameter) Food 11: 568-573.
2 µl placed on the surface of inoculated plates; duplicate plates for each 5. Azaz AD, Kürkcüoglu M, Satil F, Baser CKH, Tümen G (2005) In vitro
oil were used. After overnight incubation at 37°C, the inhibition zones antimicrobial activity and chemical composition of some Satureja essential
were measured. Control plates were prepared by placing sterile water oils. J Flav Frag 20: 587-591.
for negative controls (Figure 1 and Table 1). 6. De Martino L, Bruno M, Formisano C, De Feo V, Napolitano F, et al. (2009)
Chemical composition and antimicrobial activity of the essential oils from two
Results species of Thymus growing wild in southern Italy. Molecules 14: 4614-4624.

Proteus mirabilis were the most susceptible, followed by Klebsiella 7. Sienkiewicz M, Lysakowska M, Ciecwierz J, Denys P, Kowalczyk E (2011)
pneumonia and Morganella morganii, Pseudomonas aeruginosa did not Antibacterial activity of thyme and lavender essential oils. Med Chem 7: 674-
689.
show susceptibility to any oil. Klebsiella pneumonia susceptible, other
oils did not show susceptibility to the camphor oil. 8. Rota MC, Herrera A, Martínez RM, Sotomayor JA, Jordán MJ (2008)
Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus
Discussion zygis and Thymus hyemalis essential oils. Food Control 19: 681-687.

In the literature, the results of experiments showed that the oil from 9. Abu-Shanab B, Adwan GM, Abu-Safiya D, Jarrar N, Adwan K (2005)
Antibacterial activities of some plant extracts utilized in popular medicine in
Thymus exhibited extremely strong activity against all of the clinical Palestine. Turkish J Biol 28: 99-102.
strains. In traditional popular medicine, Thymus spp. essential oil has
been traditionally used to treat respiratory tract disorders and wound 10. Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: Antibacterial
activity of plant volatile oils. J Appl Microbiol 88: 308-316.
infections; using of Thymus derivatives has been used to treat wound
infections, cough, and sputum treatment. Over the past few years, 11. Skandamis P, Koutsoumanis K, Nychas GJE, Fasseas K (2001) Inhibition
the interest in natural medicine has been increasing in industrialized of oregano essential oil and EDTA on Escherichia coli O157: H7 [Food
hygiene]. Italian J Food Sci.
societies and in medical area also. It is evident that there is a relationship
between the high activity of thymol oil and the presence of phenol 12. Carson CF, Mee BJ, Riley TV (2002) Mechanism of action of Melaleuca
components such as thymol and carvacrol to the bacterias [7-11]. alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill,
lysis, leakage, and salt tolerance assays and electron microscopy. Antimicrob
According to literatures, the mode of action of essential oils against Agents Chemoth 46: 1914-1920.

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ISSN: 1747-0862 Volume 12 • Issue 1 • 1000318

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