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    Drboloon

    Uploaded by

    Reno Romzul Fauzi
    The document contains data from an experiment measuring the rate of a reaction (s) at different substrate concentrations (v) and with different concentrations of an inhibitor. A line is fitted to a double reciprocal plot of 1/s and 1/v, from which the maximum reaction rate (vmax) and Michaelis constant (Km) are determined. Additional data shows the effect of increasing inhibitor concentration on the Michaelis constant and maximum reaction rate.

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    © All Rights Reserved
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    Drboloon

    Uploaded by

    Reno Romzul Fauzi
    13 views5 pages
    The document contains data from an experiment measuring the rate of a reaction (s) at different substrate concentrations (v) and with different concentrations of an inhibitor. A line is fitted to a double reciprocal plot of 1/s and 1/v, from which the maximum reaction rate (vmax) and Michaelis constant (Km) are determined. Additional data shows the effect of increasing inhibitor concentration on the Michaelis constant and maximum reaction rate.

    Original Description:

    inhibitor enzim

    Original Title

    drboloon

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    The document contains data from an experiment measuring the rate of a reaction (s) at different substrate concentrations (v) and with different concentrations of an inhibitor. A line is fitted to a double reciprocal plot of 1/s and 1/v, from which the maximum reaction rate (vmax) and Michaelis constant (Km) are determined. Additional data shows the effect of increasing inhibitor concentration on the Michaelis constant and maximum reaction rate.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    13 views5 pages

    Drboloon

    Uploaded by

    Reno Romzul Fauzi
    The document contains data from an experiment measuring the rate of a reaction (s) at different substrate concentrations (v) and with different concentrations of an inhibitor. A line is fitted to a double reciprocal plot of 1/s and 1/v, from which the maximum reaction rate (vmax) and Michaelis constant (Km) are determined. Additional data shows the effect of increasing inhibitor concentration on the Michaelis constant and maximum reaction rate.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    of 5

    [S] V 1/s 1/v

    0 0
    0.02 10.83 50 0.0923
    0.04 18.57 25 0.0539
    Chart Title
    0.07 26.76 14.29 0.0374
    0.1 32.5 10 0.0308 60
    0.15 39 6.67 0.0256
    0.2 43.33 5 0.0231 50
    0.3 48.75 3.33 0.0205
    0.5 54.17 2 0.0185 40
    0.7 56.8 1.43 0.0176
    30

    20
    intercept 0.0154
    slope 0.0015 10
    r 1
    0
    0 0.1 0.2 0.3 0.4 0.5
    Chart Title Chart Title
    0.1000
    0.0900
    0.0800
    0.0700
    0.0600
    0.0500
    0.0400
    0.0300
    0.0200
    0.0100
    0.0000
    0 10 20 30 40 50 60
    0.3 0.4 0.5 0.6 0.7 0.8
    40 50 60
    inhibitor v s 1/v 1/s
    1g/L 0.909 20 1.10011 0.05
    0.658 10 1.519757 0.1 inhibitor
    Km 0.493 6.67 2.028398 0.149925 5
    9.2 0.4 5 2.5 0.2 4.5
    0.333 4 3.003003 0.25 4
    0.289 3.33 3.460208 0.3003 3.5
    0.227 2.5 4.405286 0.4 3
    2.5
    2
    1.5
    1
    vmax 1.6639 slope 9.5225 0.5
    km 15.8445 intercept 0.6010 0
    0 0.05 0.1 0.15 0.2 0.25 0

    0.717
    inhibitor

    0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45

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