1.00579.

0500 Sample material

Starting materials are

1.07960.0500 • formalin-fixed, paraffin-embedded, histologically stained tissue specimens

(3 - 5 µm thick paraffin sections)
• fixed and stained cytological smears, e. g. sputum, fine needle aspiration biop-
1.07961.0100 sies (FNAB), rinses, imprints, effusions
• air-dried, heat-fixed, and stained smears of bacteriological specimen material,
1.07961.0500 e. g. liquid and solid enrichment cultures of bacteria from body fluids, exsu -
dates, pus

1.00869.0500 • hematologically processed and stained blood or bone-marrow smears from all
regions of the human body.

1.01691.0025 Reagents
Cat. No. 100579 DPX new 500 ml
1.01691.0100

water-free mounting medium
for microscopy
1.03973.0001 Cat. No. 107960

Entellan®
rapid mounting medium
500 ml

1.09016.0100
Cat. No. 107961
for microscopy
Entellan® new 100 ml, 500 ml,
rapid mounting medium 1l
1.09016.0500
Cat. No. 100869
for microscopy
Entellan® new for cover slipper 500 ml
for microscopy
Microscopy Cat. No. 101691 Canada balsam 25 ml, 100 ml
for microscopy

DPX new Cat. No. 103973

M-GLAS®
liquid cover glass
500 ml

water-free mounting medium for microscopy for microscopy

Cat. No. 109016 Neo-Mount® 100-ml dropping
Entellan®

anhydrous mounting medium
for microscopy
bottle, 500 ml
rapid mounting medium for microscopy
Entellan® new Specifications
Cat. No. 100579 DPX new, water-free mounting medium for microscopy
rapid mounting medium for microscopy
is a water-free mounting medium for microscopy, in which the teratogenic ingredi-
Entellan® new for cover slipper ent Dibutyl phthalate (DBP) has been avoided.
Refractive index (20°C) 1.518 - 1.521
for microscopy
Viscosity (20°C) 600 - 700 mPa*s
Canada balsam Cat. No. 107960 Entellan®, rapid mounting medium for microscopy
for microscopy is a water-free mounting medium for microscopy for the permanent mounting
of specimen is an anhydrous mounting medium for microscopy that is used for
M-GLAS® the permanent mounting and storage of specimens, and consists of a polymer
liquid cover glass for microscopy made of mixed acrylates dissolved in toluene. As it contains toluene, it should be
used with water-free specimens that have been processed with xylene previous
Neo-Mount® mounting.
Refractive index (20°C) 1.492 - 1.500
anhydrous mounting medium for microscopy Density (20°C/4°C) 0.925 - 0.935 g/cm3
Viscosity (20°C) 60 - 100 mPa*s
In Vitro Diagnostic Medical Device Fluorescence < 100 ppb

Cat. No. 107961 Entellan® new, rapid mounting medium for microscopy
These ready-to-use anhydrous mounting media are suited for mounting dehydrat- is a water-free mounting medium for microscopy that consists of a polymer of
ed sample material of human origin after these have been fixed and embedded mixed acrylates which are solubilized in xylene. Therefore, it should be used with
as necessary, and then histologically, bacteriologically, hematologically (enzyme- specimens that have been cleared with xylene previous mounting.
cytochemically), or cytologically stained and, where applicable, counterstained Refractive index (20°C) 1.490 - 1.500
with other in vitro diagnostic products from our portfolio, thus making them evalu-
able for further diagnostic procedures. Samples are mounted on slides to enable Density (20°C/4°C) 0.94 - 0.96 g/cm3
the specimen material to be examined by light microscopy, at the same time Viscosity (20°C) 250 - 600 mPa*s
preserving it and thus enabling it to be re-examined many years later.
The appropriate anhydrous mounting medium for the respective application is Cat. No. 100869 Entellan® new for cover slipper for microscopy
given in the corresponding instructions for use for our In Vitro Diagnostic staining is a mounting medium for microscopy that is especially suited for standard
solutions, solid dyes, and test kits. commercial automated-mounting instruments that operate with glas coverslips.
It is used as described in the instruction manual for cover slippers and the ideal
amount of mounting agent is determined in a pilot run. There, empty cover glass-
Principle es and specimen holders, according to the size of the cover glass and the size
Mounting media are viscous, clear liquids with brilliant light-refraction properties. and thickness of the specimen, are used and these conditions are re-checked
They are either won from natural materials or else are made e.g. of acryl-resin when a new bottle of the mounting medium is used. As its viscosity range is
mixtures that are dissolved in aromatic solvents such as xylene or toluene. adjusted to a narrow range, the effort for new the calibration of the instrument is
In the last steps of the staining process prior to mounting, the still aqueous, minimized.
stained specimen slides pass through a series of baths with ascending alcohol
Refractive index (20°C) 1.490 - 1.500
concentrations, ultimately ending up in an anhydrous solvent that is also referred
to as an intermedium, e.g. toluene, xylene, or a xylene substitute (e.g. Neo-Clear®, Viscosity (20°C) 500 - 600 mPa*s
Cat. No. 109843).
The anhydrous mounting media in their dissolved form are then dropped onto the Cat. No. 101691 Canada balsam for microscopy
stained and dehydrated specimen of human origin, and the slide is covered air- is a commonly used mounting medium for microscopy to prepare permanent
tight with a cover glass. The evaporation of the intermedium causes the mounting slides. It is produced from the resin of the balsam fir tree and its use can be
medium to harden, forming a solid, clear film under the cover glass, preserving combined with xylene-containing specimens.
the stained specimen material and thus enabling it to be kept for several years Refractive index (20°C) 1.515 - 1.530
for re-analysis at a later date. As a result of the glass-similar refractive properties
Density (20°C/4°C) 0.980 g/cm3
of the cover glass, the sample can now be observed under a microscope without
any interference. Viscosity (20°C) 3000 mPa*s
Thanks to the practical user-friendly dropping bottle, the mounting medium can
be easily and safely dropped onto the slide without smearing. The closure of the
nozzle ensures that the viscosity of the medium remains constant, meaning that
the mounting medium is immediately ready for use.
Cat. No. 103973 M-GLAS®, liquid cover glass for microscopy Note
is used in cytology instead of a cover glass to ensure that the stained specimens In the case of mounted specimens, the cover slips can be detached again by
are homogenously covered. A few drops are applied onto the specimen, taking immersing them in xylene. Specimens that have been mounted with M-GLAS®
care that the mounting medium is evenly distributed over the specimen material. (Cat. No. 103973) can also be treated in this manner.
After the solvent has evaporated, a solid, protective lacquer film remains that
ensures that the specimen material is preserved. Cat. No. 100579 DPX new, water-free mounting medium for microscopy
Refractive index (20°C) 1.490 - 1.500 Solvent Xylene
Density (20°C/4°C) 0.980 g/cm3 Immersion time approx. 65 hours
Viscosity (20°C) 500 - 600 mPa*s
< 250 ppb Cat. No. 107960 Entellan®, rapid mounting medium for microscopy
Fluorescence
Solvent Xylene
Cat. No. 109016 Neo-Mount®, anhydrous mounting medium for microscopy Immersion time approx. 24 hours
is an extremely color-stable mounting medium for microscopy, which is pro-
Cat. No. 107961 Entellan® new, rapid mounting medium for microscopy
duced with solvents based on mixtures of aliphatic hydrocarbons. It contains an
aromatic-free substitute for xylene, thus, Neo-Mount® needs to be combined with Solvent Xylene
Neo-Clear® (Cat. No. 109843) exclusively. Xylene must be avoided in the mount- Immersion time approx. 72 hours
ing step, as it will cause the slides to become cloudy and streaked. The applica-
tion of Neo-Mount® is not recommended in fluorescence microscopy for clinical Cat. No. 100869 Entellan® new for cover slipper for microscopy
diagnostics. In addition, by placing the dehydrated slides on filter paper for ap- Solvent Xylene
prox. 1 minute prior mounting, any excess of Neo-Clear® could be circumvented,
as air bubbles might arise under the coverslip. The same precondition should Immersion time approx. 72 hours
also be met when mounting specimens using cover-slip machines; in this area,
Neo-Clear® can be most efficiently eliminated by incubation of the slides for one Cat. No. 101691 Canada balsam for microscopy
minute in an empty slide rack. Solvent Xylene
Refractive index (20°C) 1.417 - 1.465 Immersion time approx. 51 hours
Viscosity (20°C) 250 - 350 mPa*s
Cat. No. 103973 M-GLAS®, liquid cover glass for microscopy
Also required: Solvent Xylene
Cat. No. 100974 Ethanol denatured with about 1 l, 2.5 l Immersion time approx. 17 hours
1 % methyl ethyl ketone for analysis
EMSURE® Cat. No. 109016 Neo-Mount®, anhydrous mounting medium for microscopy
Cat. No. 108298 Xylene (isomeric mixture) 4l Solvent Xylene
for histology Immersion time approx. 24 hours
Cat. No. 109843 Neo-Clear® (xylene substitute) 5 l, 25 l
for microscopy Result
The use of these anhydrous, ready-to-use mounting media results in completely
Sample preparation airtight specimen slides, the structure and stain pattern of which remains pre-
The sampling must be performed by qualified personnel. served over the long term, enabling them to be microscopically re-analyzed at a
later date.
All samples must be treated using state-of-the-art technology.
All samples must be clearly labeled.
Suitable instruments must be used for taking samples and their preparation. Fol- Trouble-shooting
low the manufacturer’s instructions for application / use. Oil-drop-shaped artefacts on the slide
The specimen material is processed, stained (and counterstained where applica-
ble), and mounted according to the instructions for use of our In Vitro Diagnostic
• When exchanging one anhydrous mounting medium in a cover slipper for
another, e. g. when switching from Entellan® to Entellan® new, it is absolutely
staining solutions, solid dyes, and test kits. imperative to rinse the entire injection system of the cover slipper with the
Histological and cytological specimens must be completely dehydrated before solvent xylene before using the new mounting medium. Only then can the new
mounting. In the last stage, either xylene or a xylene substitute should be used to mounting medium be used.
prevent the occurrence of turbidity due to aqueous solutions.
Hematological and bacteriological specimens must be dries completely before Turbidity of the slides
microscopy using immersion oil, i. e. allow specimen slides to dry thoroughly or,
if necessary, mount with an anhydrous mounting medium and cover glass, other-
• As a measure to ensure that the specimen slides retain optimal optical proper-
ties and their transparency, in all cases a mounting medium must be used that
wise the microscopic image will become turbid. is based on the solvent/intermediate used for the clarification process. The
Neo-Mount ® mounting medium is, for example, not compatible with xylene
Reagent preparation and hence should be used only in combination with the intermediate
All listed anhydrous mounting media are ready-to-use, dilution of the mounting Neo-Clear®.
media is not necessary.
No color stability over longer storage times
When exchanging one anhydrous mounting medium in a cover slipper for
another, e. g. when switching from Entellan® to Entellan® new, it is absolutely • A minimum quality of the solvents must be observed.
Technical-grade solvents may have a relatively high water content, which may
imperative to rinse the entire injection system of the cover slipper with the solvent
xylene before using the new mounting medium. Only then can the new mounting result in incomplete dehydration and hence in the stained specimen becoming
medium be used. turbid or decolorized.
If this is not done, oil-drop-shaped artefacts will form on the slide. • A minimum quality and staining-dye content must be observed to ensure that
the staining pattern of the specimens remains stable over the long term.
Procedure
Air bubbles and inclusions
The mounting medium must contain the same solvent, used for the water-
clearing procedure to obtain the optimal optical properties and transparency of • In all cases a mounting medium must be used that is based on the solvent /
intermediate used for the clarification process.
the slides.
All mounting procedures should be carried out in a fume hood. • The volume of the mounting medium applied to the specimen must be care-
fully monitored (to avoid too much or too little mounting medium).
The mounting medium is applied to the horizontal slide, using a glass rod or else
directly dropping approx. 0.2 ml of one of the listed mounting media from the • The drying times for the specimens must be observed.
The specimens must be completely dehydrated before microscopy with im-
dropping bottle. As soon as a homogeneous distribution of the solution is guar-
mersion oil, i. e. always allow the specimens to dry completely and mount
anteed, gently add a clean cover glass, so that the space between the slide and
thoroughly.
cover glass is filled without air bubbles with mounting medium. Allow this setup to
dry and harden for about 20 - 30 min in a horizontal position. • The evaporation of the solvent after mounting must be borne in mind, and
specimen slides must be dried for at least 20 - 30 min.
When pre-treated in the correct manner, the color of the specimens remains
stable for at least five years.
Technical notes
The use of immersion oil is recommended for the analysis of stained slides with a The microscope used should meet the requirements of a medical diagnostic
microscopic magnification >40x. laboratory.
When using automated-mounting instruments, please follow the instructions for
use supplied by the supplier of the system and software.
Remove surplus immersion oil before filing.
Diagnostics Cat. No. 103973
Diagnoses are to be made only by authorized and trained personnel. Polymer of mixed acrylates in 73.3 % (w/w) toluene
Valid nomenclatures must be used. 1 l = 0.91 kg
Further tests must be selected and implemented according to recognized methods.
Suitable controls should be conducted with each application in order to avoid an Cat. No. 109016
incorrect result.
Polymer of mixed acrylates in 64 % (w/w) Shellsol 140/165

Storage Other IVD products

Cat. Nos. 100579, 107960, 107961, 100869, 103973, 109016:
Cat. No. 100496 Formaldehyde solution 4%, 350 ml and
Store the listed mounting media at +15 °C to +25 °C.
buffered, pH 6.9 (approx. 10% Formalin 700 ml (in bottle
Cat. No. 101691: solution) with wide neck),
Store the Canada balsam for microscopy at +5 °C to +30 °C. for histology 5 l, 10 l,
10 l Titripac®
Shelf-life Cat. No. 101646 PAS staining kit 2x 500 ml
Cat. Nos. 100579, 107960, 107961, 100869, 103973, 109016: for detection of aldehyde and
The listed mounting media can be used until the stated expiry date. mucosubstances
After first opening of the bottle, the contents can be used up to the stated expiry Cat. No. 102480 Eosin-Phloxine solution, alcoholic 500 ml, 1 l
date when stored at +15 °C to +25 °C. for microscopy
Cat. No. 101691: Cat. No. 105174 Hematoxylin solution modified acc. to Gill III 500 ml, 1 l, 2.5 l
The Canada balsam for microscopy can be used until the stated expiry date. for microscopy
After opening the bottle the first time and subsequent storage of the tightly re- Cat. No. 109204 Giemsa’s azur eosin methylene blue 100 ml, 500 ml,
closed bottle at +5°C to +30 °C, the medium can be used up to the printed expiry solution 1 l, 2.5 l
date. for microscopy
Cat. No. 111609 Histosec® pastilles 1 kg, 10 kg (4x
Additional instructions soldification point 56-58°C 2.5 kg), 25 kg
For professional use only. embedding agent for histology
In order to avoid errors, the application must be carried out by qualified personnel Cat. No. 111885 Gram-color stain set 1 set
only. for the Gram staining method
National guidelines for work safety and quality assurance must be followed. Cat. No. 115161 Histosec® pastilles (without DMSO) 10 kg (4x 2.5 kg),
Microscopes equipped according to the standard must be used. soldification point 56-58°C 25 kg
embedding agent for histology
Protection against infection
Effective measures must be taken to protect against infection in line with labora- Literature
tory guidelines. 1. Löffler, H., Rastetter, J., Haferlach, T, Atlas der klinischen Hämatologie, 2004,
Springer-Verlag Berlin Heidelberg
Instructions for disposal 2. Routine Cytological Staining Techniques: Theoretical Background and Practice,
The package must be disposed of in accordance with the current disposal Mathilde E. Boon, Johanna S. Drijver, 1986, Elsevier Science Publishing
guidelines. Company
Used solutions and solutions that are past their shelf-life must be disposed 3. Romeis - Mikroskopische Technik, Editors: Mulisch, Maria, Welsch, Ulrich,
of as special waste in accordance with local guidelines. Information on dis- 2015, Springer-Verlag Berlin Heidelberg
posal can be obtained under the Quick Link “Hints for Disposal of Microscopy 4. Theory and Practice of Histological Techniques, John D Bancroft and Marilyn
Products” at www.microscopy-products.com. Within the EU the currently applica- Gamble, 6th Edition
ble REGULATION (EC) No 1272/2008 on classification, labelling and packaging
of substances and mixtures, amending and repealing. Directives 67/548/EEC and 5. Theory and application of Microbiological Assay, Hewitt, W. and Vincent, S.,
1999/45/EC, and amending Regulation (EC) No 1907/2006 applies. 1989, Academic Press
6. Conn’s Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for
Use in Biology and Medicine, 10th Edition, (ed. Horobin, R.W. and Kiernan,
Auxiliary reagents
J.A). Bios, 2002
Cat. No. 100974 Ethanol denatured with about 1 l, 2.5 l
1 % methyl ethyl ketone for analysis
EMSURE®
Cat. No. 100983 Ethanol absolute for analysis EMSURE® 1 l, 2.5 l, 5 l
ACS, ISO, Reag. Ph Eur
Cat. No. 104699 Immersion oil 100-ml dropping
for microscopy bottle, 100 ml,
500 ml
Cat. No. 108298 Xylene (isomeric mixture) 4l
for histology
Cat. No. 109843 Neo-Clear® (xylene substitute) 5 l, 25 l
for microscopy

Hazard classification
Cat. Nos. 100579, 107960, 107961, 100869, 101691, 103973, 109016
Please observe the hazard classification printed on the label and the information
given in the safety data sheet.
The safety data sheet is available on the website and on request.

Main components of the products

Cat. No. 100579
Copolymer in 70 % (w/w) xylene

Cat. No. 107960 Consult instructions Manufacturer Catalog number Batch code
Mixed acrylate in 75 % (w/w) toluene for use
1 l = 0.93 kg

Cat. No. 107961

Polymer of mixed acrylates in 60 % (w/w) xylene Caution, consult Use by Temperature
accompanying documents YYYY-MM-DD limitation
1 l = 0.95 kg

Cat. No. 100869 Status: 2017-11-29

Polymer of mixed acrylates in 60 % (w/w) xylene
1 l = 0.95 kg Merck KGaA, 64271 Darmstadt, Germany
Tel. +49(0)6151 72-2440
Cat. No. 101691 www.microscopy-products.com
CAS-No 8007-47-4
1 l = 0.98 kg EMD Millipore Corporation, 290 Concord Road, Billerica,
MA 01821, USA, Tel. +1-978-715-4321