Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                

Don't Trust Me

Download as docx, pdf, or txt
Download as docx, pdf, or txt
You are on page 1of 2

P a g e |1

Carbohydrates
Mark Ian Go Pascua

University of the Philippines Diliman, Quezon City, Philippines


Performed Oct 9 2019; Submitted 25 Oct 2019

ANSWERS TO QUESTIONS

1. Draw the structures of the osazone produced in the experiment.

Glucosazone

2. Arrange the following sugars according to their reactivity with Benedict’s Reagent. Explain your answer.
 Sucrose<starch<glucose=fructose<maltose. The arrangement is according to the presence of
hemiacetals in their structures, i.e. ketones and aldehydes which directly affect the reducing power
of the sugar[1].
3. What are the differences between Benedict’s and Barfoed’s test?
 Both Benedict’s and Barfoed’s test tests for reducing sugars, however Barfoed’s reagent only gets a
positive result from monosaccharides. Benedict’s test takes 15 minutes, while Barfoed’s test only
takes 10 minutes[1][2].
4. Can the Seliwanoff’s test be used to differentiate sucrose from fructose? If yes how?
 Seliwanoff’s test cannot be used differentiate sucrose from fructose because sucrose contains both
ketone and aldehyde groups which would cause it to react quickly with the reagent; fructose, which
is a ketose, will react quickly. Because both would react quickly one cannot use the test to
differentiate the two[3].
5. Give a possible application of Bial’s test.
 The Bial’s test tests for the presence of 5 chained cyclic sugars, therefore in industries that use
furanoses, such as the food industry with fructose, the Bial’s test can be used to test if a compound is
a good source of furanoses.
6. Draw the hydrolysis products of sucrose and starch
Sucrose products:
Glucose Fructose

Mark Ian Go Pascua. Mobile: 09262990344


E-mail Add:leoriser@gmail.com
P a g e |2

Starch products:
Glucose

7. Give a chemical explanation for the role of starch as an antidote for iodine poisoning.
 Iodine and starch form a blue-black complex, the way that Iodine and starch react is that iodine can
fit into the helix configuration of the starch, which it does. The formation of the complex then makes
the iodine less susceptible to getting absorbed by the body.

REFERENCES

[1]Fine, J. 1935. Benedict’s Qualitative Test A FURTHER MODIFICATION SUITABLE FOR ESTIMATION OF URINE
GLUCOSE IN WARD OR SIDE ROOM. Br. Med. J., 1169-1170.
[2]Roaf, H. 1908. On the Application of Barfoed's Reagent to show the Hydrolysis of Disaccharides by Enzymes
. Biochem J., 182-184
[3] Werteim E. 1950. Experiments in Organic Chemistry with Some Biochemistry.
Blackinston Co., Canada.
[4]Bial, M 1902. Die Diagnose der Pentosurie 28(15): 253-254DOI: 10.1055/s-0029-1203493

You might also like