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    Study of Plant Cells in Hypertonic Solution: Experiment: Plasmolysis

    Uploaded by

    Haq Chandio
    This experiment aims to observe the effect of a hypertonic solution on plant cells. When an onion peel is placed in a concentrated salt or sugar solution, the plant cells undergo plasmolysis. [1] The plant cells lose water through exosmosis as the solution outside the cells is hypertonic compared to the cell sap. [2] This causes the cytoplasm and organelles to shrink and pull away from the cell wall. [3] The space between the cell wall and shrunken cytoplasm is filled with the hypertonic solution, demonstrating the phenomenon of plasmolysis.

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    © All Rights Reserved
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    Study of Plant Cells in Hypertonic Solution: Experiment: Plasmolysis

    Uploaded by

    Haq Chandio
    230 views2 pages
    This experiment aims to observe the effect of a hypertonic solution on plant cells. When an onion peel is placed in a concentrated salt or sugar solution, the plant cells undergo plasmolysis. [1] The plant cells lose water through exosmosis as the solution outside the cells is hypertonic compared to the cell sap. [2] This causes the cytoplasm and organelles to shrink and pull away from the cell wall. [3] The space between the cell wall and shrunken cytoplasm is filled with the hypertonic solution, demonstrating the phenomenon of plasmolysis.

    Original Description:

    Botany related experiement

    Original Title

    Plant Cells Plasmolysis expriement

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    © © All Rights Reserved

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    This experiment aims to observe the effect of a hypertonic solution on plant cells. When an onion peel is placed in a concentrated salt or sugar solution, the plant cells undergo plasmolysis. [1] The plant cells lose water through exosmosis as the solution outside the cells is hypertonic compared to the cell sap. [2] This causes the cytoplasm and organelles to shrink and pull away from the cell wall. [3] The space between the cell wall and shrunken cytoplasm is filled with the hypertonic solution, demonstrating the phenomenon of plasmolysis.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    230 views2 pages

    Study of Plant Cells in Hypertonic Solution: Experiment: Plasmolysis

    Uploaded by

    Haq Chandio
    This experiment aims to observe the effect of a hypertonic solution on plant cells. When an onion peel is placed in a concentrated salt or sugar solution, the plant cells undergo plasmolysis. [1] The plant cells lose water through exosmosis as the solution outside the cells is hypertonic compared to the cell sap. [2] This causes the cytoplasm and organelles to shrink and pull away from the cell wall. [3] The space between the cell wall and shrunken cytoplasm is filled with the hypertonic solution, demonstrating the phenomenon of plasmolysis.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Experiment : Plasmolysis

    Study of Plant cells in Hypertonic Solution

    Objective

    To observe the cells of an onion peel in hypertonic solution.

    Apparatus and material required

    An onion, glass slide, coverslip, forceps, needle, brush, blade, blotting paper, dropper,
    concentrated salt or sugar solution and a compound microscope.

    Theory

    When a plant cell is placed in a s solution that has a higher solute concentration(hypertonic
    compared to the cell sap) water is lost from the cell by osmosis (exosmosis). As the
    concentration of water molecules in the cell is higher than the outer hypertonic solution, net
    movement of water molecules is from their region of higher concentration to their region of
    lower concentration across the selectively permeable plasma membrane. This process is
    called exosmosis. If exosmosis continues in a plant cell, cytoplasm along with the nucleus
    and vacuole shrinks to a small irregular mass due to loss of water. Eventually it pulls away
    form the cell wall. The space between, the cell wall and cytoplasmic mass is filled with the
    salt or sugar solution due to the permeability of the cell wall. This phenomenon is
    plasmolysis and the cell is said to be plasmolysed.

    Plasmolysis is a reversible phenomenon. When the plasmolysed cell is placed in water,


    which is hypotonic compared to cell sap, the cell gains water due to endosmosis. As a result.
    The cell membrane, cytoplasm and vacuole regain their normal position. This phenomenon
    is called deplasmolysis.

    Procedure

    1. Remove the outermost peel of the onion.


    2. Cut out a small part from an inner fleshy scale leaf using the blade.
    3. Pull out the transparent peel of the scale leaf using the forceps.
    4. Using the brush place the piece of onion peel on a clean glass slide. Put a drop of the
    concentrated salt or sugar Solution on the peel with the help of the dropper.
    5. Put a clean coverslip by tilting it over the needle. In this way you can prevent air
    bubbles form getting trapped under the coverslip.
    6. Remove excessive solution with the blotting paper.
    7. Observe the mounted onion peel immediately under the low and high magnification
    of the compound microscope.
    8. Observe it again under the microscope after some time. Note the shrunken cytoplasm
    of the cells.

    Observations

    After putting the drop of the hypertonic solution on the cells of the onion peel, their cell
    membranes withdraw from the cell walls; the enclosed cytoplasm nucleus and vacuole lose
    water and shrink to one side of the cell. The cell walls remain at their original positions. The
    space between the cell wall and the shrunken cytoplasm is filled with the salt or sugar
    solution.
    Precautions

    1. Use a fresh onion peel.


    2. Avoid folding the peel.
    3. Do not let the peel dry out.
    4. Use a clean, dry glass slide and coverslip
    5. Avoid trapping air bubbles under the coverslip.
    6. Remove excessive solution from the slide with the blotting paper.

    These pictures are only for reference. Do not draw them in your Practical Files

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