Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                

Parasitology SIMs + Lab Manual

Download as pdf or txt
Download as pdf or txt
You are on page 1of 45

Emilio Aguinaldo Educational Corporation

School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Introduction to Medical Parasitology


Learning Objectives
1. To define Parasitology
2. To discuss the host-parasite relationships
3. To discuss the role of immunology on the host – parasite interaction
4. To recognize the parasitic groups of medical and public health importance
5. To apply the concepts of host-parasite interaction

Guide questions
1. Define parasitology, tropical medicine and tropical diseases
2. What are biologic relations?
3. What are the modes of transmission of parasites?
4. Discuss the effects of a parasite on a host and vice versa.
5. How does a host respond to parasitic invasion?
6. How does parasites evade the host immune system?
7. What are the adverse effects of immune response in the host during a parasitic infection?
8. What are the major groups of medically important parasites? Give their general characteristics.
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Diagnostic Techniques in Parasitology

Learning Objectives
1. To discuss the importance of blood, stool and body fluid analysis in medical parasitology
2. To discuss importance of tissue biopsy in medical parasitology
3. To recognize recent advances in diagnostic parasitology
4. To explain the quality assurance in a parasitology laboratory
5. To apply the techniques of diagnostic parasitology

Guide questions
1. How are blood, stool and body fluids analyzed for parasitic evaluation? Give the methods and
cite its application.
2. What are the common preservatives used for diagnostic parasitology?
3. Give the application of tissue biopsy in parasitology. Explain briefly.
4. Discuss the following diagnostic techniques and give its application.
A. Immunodiagnosis
B. Molecular diagnosis
C. Rapid diagnostic tests
5. Apply the principles of quality assurance in the performance of diagnostic techniques in
Parasitology.
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Intestinal Protozoan Infections


Learning Objectives
1. To discuss characteristics and life cycle of medically important pathogenic and
nonpathogenic intestinal protozoans
2. To discuss pathogenesis and clinical manifestations caused by intestinal protozoans
3. To discuss the diagnostic techniques utilized in the isolation and identification of intestinal
protozoans
4. To discuss the treatment modalities and management of parasitic intestinal infections
5. To discuss the prevention and control of intestinal protozoan infections
6. To apply the concepts learned in this activity in theoretical situations.

Guide questions
1. What are the general and specie specific characteristics of each of the pathogenic and
nonpathogenic intestinal protozoans?
2. What are the common and specie specific clinical manifestations of diseases caused by
intestinal protozoans? Explain the pathogenesis.
3. What are the diagnostic modalities and criteria required in the evaluation of intestinal
protozoan infections?
4. What are the treatment and management options in handling intestinal protozoan infections?
5. How are intestinal protozoan infections prevented and controlled?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Blood and Tissue Protozoan Infections


Learning Objectives
1. To discuss characteristics and life cycle of medically important blood and tissue protozoans
2. To discuss pathogenesis and clinical manifestations caused by blood and tissue protozoans
3. To discuss the diagnostic techniques utilized in the isolation and identification of blood and
tissue protozoans
4. To discuss the treatment modalities and management of parasitic blood and tissue
protozoan infections
5. To discuss the prevention and control of blood and tissue protozoan infections
6. To apply the concepts learned in this activity in theoretical situations.

Guide questions
1. What are the general and specie specific characteristics of each blood and tissue protozoan?
2. What are the common and specie specific clinical manifestations of diseases caused by blood
and tissue protozoans? Explain the pathogenesis.
3. What are the diagnostic modalities and criteria required in the evaluation of blood and tissue
protozoan infections?
4. What are the treatment and management options in handling blood and tissue protozoan
infections?
5. How are blood and tissue protozoan infections prevented and controlled?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

LABORATORY EXERCISES FOR PROTOZOANS

NAME: _________________________________________________YEAR/SECTION________

Learning Objectives

At the end of the activity, the student shall be able to:

1. Correlate the clinical history, physical examination, diagnostic tests and gross and microscopic
characteristics in the diagnosis of the given cases.

2. Using prepared slides/photographs/gross specimens, recognize following conditions:


a. Entamoeba histolytica/Entamoeba dispar
b. Giardia lamblia
c.Trichomonas vaginalis
d. Balantidium coli
e. Plasmodium spp.

3. Explain the pathogenesis and symptomatology in the clinical cases.

Evaluation

Criteria: The student


Poor Fair Good Excellent
1. Demonstrates drawings clearly and accurately 0 1 2 3
2. Completely answers guide questions 0 1 2 3
3. Accomplishes and submits laboratory manual on time 0 1 2 3
4. Participates actively in class discussion and activities 0 1 2 3
5. Answers questions correctly 0 1 2 3

Score: __________
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 1: Amoebaiasis
A 25-year old male is complaining of vague crampy abdominal pain with loose bowel movement
characterized as bloody mucoid stools since yesterday. He self-medicated with hyoscine which only
prompted temporary relief. He admits he usually eats his lunch and supper at a kitchenette in front of his
office. Currently he is feverish and still has loose bowel movement characterized as bloody mucoid with a
fishy odor.

Draw and label the life cycle

Guide questions

1. Differentiate Entamoeba histolytica from Entamoeba coli and Entamoeba dispar.

2. Discuss the diagnostic tests which may be utilized in the diagnosis of amoebiasis.

3. What are the complications of amoebiasis?

4. What are the treatment options for the different presentations of amoebiasis?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 2: Giardiasis
A 24-year-old man visits his physician complaining of significant weight loss, flatulence, and foul-
smelling stools. He reports feeling fatigued since his return from China months previously and has
suffered abdominal cramping and intermittent loose, nonbloody stools since then. The patient’s stool ova
and parasite studies demonstrated characteristic trophozoites on two occasions. He was prescribed a
course of drug therapy and warned that consumption of alcohol during treatment could lead to nausea and
vomiting.

Draw and label the life cycle

Guide questions

1. Describe the movement and appearance of this protozoan.

2. What are the diagnostic tests which may be utilized to diagnose giardiasis?

3. How is this condition managed?

4. Discuss the pathogenesis of giardiasis.


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 3: Trichomoniasis
A 30-year-old woman presents to the clinic with complaints of increased frothy vaginal discharge.
She is married and is a full-time house wife. Speculum examination shows the cervix is strawberry red
with whitish discharge. A urinalysis and pap smear is done. The urinalysis showed a flagellated
trophozoite with a jerky movement while the pap smear showed flagellated trophozoites containing
siderophile granules.

Draw and label the life cycle

Guide questions

1. Morphologically differentiate Trichomonas vaginalis from other Trichomonas species.

2. What are the possible modes of transmission of Trichomonas vaginalis?

3. How are Trichomonas vaginalis infections adequately managed?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 4: Balantidiasis
A 45-year-old farmer sought consult due to persistent loose bowel movement associated with crampy
abdominal pain. He has recently shifted his livelihood from mostly vegetable production to hog raising.
At presentation, he looks weak and febrile. Fecalysis and urinalysis were requested. Significant finding is
a large ciliated protozoan was noted in his stool.

Draw and label life cycle

Guide questions

1. Discuss the pathogenesis of balantidiasis.

2. What are the preventive measures to avoid balantidiasis?

3. What are the reservoirs of Balantidium coli?

4. How is this condition managed?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 5: Malaria
While doing a rotation in Palawan, a medical student encounters a patient who has been having nearly
continuous high-grade fevers with occasional chills and sweats. Physical examination reveals a palpable
spleen. A drop of the patient’s blood placed in a copper sulfate solution reveals anemia. Over the next
few days, while waiting for medication to arrive, the patient’s level of consciousness waxes and wanes,
and he is somnolent at times.

Draw and label the life cycle

Guide questions

1. When is the best time to get the peripheral smear in cases of suspected malaria?

2. Differentiate the five (5) species of malaria (P. knowlesi, P. malariae, P. vivax, P. ovale, P. falciparum)
according to stages found in blood, appearance of erythrocyte and appearance of parasite in thin smear.

3. Enumerate the diagnostic procedures that may be used in the diagnosis of malaria.

4. How is malaria medically managed?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Intestinal Nematodes
Learning Objectives
1. To discuss characteristics and life cycle of medically important intestinal nematodes
2. To discuss pathogenesis and clinical manifestations caused by intestinal nematodes
3. To discuss the diagnostic techniques utilized in the isolation and identification of intestinal
nematodes
4. To discuss the treatment modalities and management of intestinal nematodes
5. To discuss the prevention and control of intestinal nematodes
6. To apply the concepts learned in this activity in theoretical situations.

Guide questions
1. What are the general and specie specific characteristics of each intestinal nematodes?
2. What are the common and specie specific clinical manifestations of diseases caused by
intestinal nematodes? Explain the pathogenesis.
3. What are the diagnostic modalities and criteria required in the evaluation of intestinal
nematodes infections?
4. What are the treatment and management options in handling intestinal nematodes infections?
5. How are intestinal nematodes infections prevented and controlled?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Blood and Tissue Nematodes


Learning Objectives
1. To discuss characteristics and life cycle of medically important blood and tissue nematodes
2. To discuss pathogenesis and clinical manifestations caused by blood and tissue nematodes
3. To discuss the diagnostic techniques utilized in the isolation and identification of blood and
tissue nematodes
4. To discuss the treatment modalities and management of parasitic blood and tissue
nematodes
5. To discuss the prevention and control of blood and tissue nematodes
6. To apply the concepts learned in this activity in theoretical situations.

Guide questions
1. What are the general and specie specific characteristics of each blood and tissue nematodes?
2. What are the common and specie specific clinical manifestations of diseases caused by blood
and tissue nematodes? Explain the pathogenesis.
3. What are the diagnostic modalities and criteria required in the evaluation of blood and tissue
nematode infections?
4. What are the treatment and management options in handling blood and tissue nematode
infection?
5. How are blood and tissue nematodes infections prevented and controlled?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

LABORATORY EXERCISES FOR NEMATODES

NAME: _________________________________________________YEAR/SECTION________

Learning Objectives

At the end of the activity, the student shall be able to:

1. Correlate the clinical history, physical examination, diagnostic tests and gross and microscopic
characteristics in the diagnosis of the given cases.

2. Using prepared slides/photographs/gross specimens, recognize following conditions:


a. Ascariasis
b. Ancylostomiasis, Necatoriasis, Uncinariasis
c. Strongyloidiasis, Cochin-China diarrhea
d. Enterobiasis, oxyuriasis
e. Trichiuriasis, trichocephaliasis
f. Pudoc’s disease, Mystery disease, Capillariasis
g. Trichinella spiralis
h. Wuchereria bancrofti
i. Brugia malayai

3. Explain the pathogenesis and symptomatology in the clinical cases.

Evaluation

Criteria: The student


Poor Fair Good Excellent
1. Demonstrates drawings clearly and accurately 0 1 2 3
2. Completely answers guide questions 0 1 2 3
3. Accomplishes and submits laboratory manual on time 0 1 2 3
4. Participates actively in class discussion and activities 0 1 2 3
5. Answers questions correctly 0 1 2 3

Section Score: __________


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 1: Ascariasis
A 45-year-old woman from Pampanga presents with diffuse, crampy abdominal pain that has persisted for
the previous 3 days. She has had no bowel movements since the pain started and has noticed a weight
loss of about 3.0 kgs over the past month. Stool sample reveals rough-surfaced eggs. Complete
blood count and liver function test results are as follows:
White blood cell (WBC) count: 16,000/mm³ Albumin: 3.0 g/dL
(30% eosinophils) Aspartate transaminase (AST): 25 IU/L
Hemoglobin: 10 g/dL Alanine transaminase (ALT): 25 IU/L
Hematocrit: 28% Alkaline phosphatase: 300 IU/L
Platelet count: 190,000/mm3 Bilirubin, total: 3.0 mg/dL
Bilirubin, direct: 3.0 mg/dL

Draw and label the life cycle

Guide questions

1. What is the infective stage of Ascaris in man?

2. What are the diagnostic techniques applicable in diagnosis of ascariasis?

3. What is the drug of choice for severe ascariasis?

4. What are available preventive measures against ascaris infection?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 2: Ancylostomiasis
An 8-year-old boy is brought to the local health center by her mother. Her mother reports the boy seems
very small for his age and has been continually lethargic for quite some time. Physical examination
reveals a small boy with a thin, scaphoid abdomen. Relevant laboratory findings are as follows:
Hematocrit: 32%
Mean corpuscular volume: 70 fL
WBC count: 12,000/mm3
Differential: 30% segmented cells, 1% bands, 30% lymphocytes, 39% eosinophils

Draw and label the life cycle

Guide questions

1. What are the different species of hookworms and how are differentiated form one another?

2. How does hookworm infections cause anemia?

3. What are the diagnostic methods utilized in diagnosing hookworm infections?

4. How is this condition properly managed?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 3: Strongyloidiasis
A 26-year-old man from Tondo presents with abdominal pain and diarrhea of 4 days’ duration. He
denies nausea, vomiting, or fever. He has no sick contacts or significant travel history. A complete blood
count shows eosinophilia. A stool sample reveals larva. On further questioning, he describes that he
frequently gardens in his neighbor’s backyard barefooted.

Draw and label the life cycle

Guide questions

1. Differentiate the rhabditiform larva and filariform larva from those of hookworms.

2. What are autoinfection and hyperinfection syndromes in strongylodiasis?

3. What tests can confirm the diagnosis of strongyloidiasis?

4. How is this complete managed and prevented?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 4: Enterobiasis
A 4-year-old girl is brought to the pediatrician because of perianal itching, which is worse at night. She
attends preschool during the day, where she shares toys and play areas with other children. The patient’s
mother recalls her daughter playing with another child who had been “scratching his backside” and
wonders
if there is a connection.

Draw and label the life cycle

Guide questions

1. What are the possible modes of transmission of this helminth?

2. Describe the Scotch Tape Swab Method or Adhesive tape test.

3. What sequelae may this infection have?

4. How is this condition properly managed and prevented?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 5: Trichiuriasis
A 10-year-old male presents with bloody diarrhea associated with abdominal pain, nausea and vomiting
and weight loss. He is also pale-looking and with some signs of dehydration. On physical examination,
his rectum seems to prolapse thru his anus.

Draw and label the life cycle

Guide questions

1. What are the factors which will determine the severity of infection?

2. How did rectal prolapse occur in this patient?

3. How Is this infection managed and prevented?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 6: Pudoc’s disease


A 27-year-old man from Ilocos presented with weight loss, chronic diarrhea and abdominal pain. He
recalled that his diet is frequently raw fish (common blackish goby). Stool examination reveals small
striated ova with inconspicuous polar plugs.

Draw and label the life cycle

Guide questions

1. What is the infective stage in man?

2. Explain the diarrhea associated with capillariasis.

3. How is this condition properly managed and prevented?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 7: Trichinosis
A 45-year-old man was admitted in emergency room for headache, fever, general muscle aches, and
weakness. Laboratory work-up on his blood specimen revealed significantly elevated eosinophilia. The
patient reported that he had gone on a camping and hunting trip the month before in the mountains. He
shot and killed a wild and brought several pounds of meat home. In the weeks before onset of symptoms,
he ate several meals that included wild boar meat.

Draw and label the life cycle

Guide questions

1. What other diagnostic tests may be utilized to diagnose this patient adequately?

2. What usually happens to the parasite in low level infections?

3. What type of muscle can this parasite encyst and develop?

4. Based on severity, how is this managed and prevented?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 8: Elephantiasis
A 52-year-old man from Mindoro presents to his physician with extreme swelling of his legs and scrotum.
The skin associated with the swollen areas is thick and scaly. The patient admits to an episode of fever
associated with enlarged inguinal lymph nodes some time ago.

Draw and label the life cycle

Guide questions:

1. Differentiate the six (6) different species of filarial worms based on: periodicity, presence or absence of
sheath, posterior end curves and vectors.

2. What are the complications of filariasis

3. What diagnostic procedures are available to diagnose this condition?

4. Discuss the pathogenesis of filariasis.

5. Discuss the management and prevention of this condition.


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Cestodes
Learning Objectives
1. To discuss characteristics and life cycle of cestodes
2. To discuss pathogenesis and clinical manifestations caused by medically important cestodes
3. To discuss the diagnostic techniques utilized in the isolation and identification of cestodes
infections
4. To discuss the treatment modalities and management of cestodes infections
5. To discuss the prevention and control of cestode infections
6. To apply the concepts learned in this activity in theoretical situations.

Guide questions
1. What are the general and specie specific characteristics of each cestode?
2. What are the common and specie specific clinical manifestations of diseases caused by
cestodes? Explain the pathogenesis.
3. What are the diagnostic modalities and criteria required in the evaluation cestode infections?
4. What are the treatment and management options in handling cestode infection?
5. How are cestodes prevented and controlled?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

LABORATORY EXERCISES FOR CESTODES

NAME: _________________________________________________YEAR/SECTION________

Learning Objectives

At the end of the activity, the student shall be able to:

1. Correlate the clinical history, physical examination, diagnostic tests and gross and microscopic
characteristics in the diagnosis of the given cases.

2. Using prepared slides/photographs/gross specimens, recognize following conditions:


a. Diphyllobothriasis
b. Taeniasis solium/cysticercosis
c. Taeniasis saginata
d. Hymenolepiasis nana/Hymenolepis diminuta
e. Diphylidiasis
f. Hydatid disease

3. Explain the pathogenesis and symptomatology in the clinical cases.

Evaluation

Criteria: The student


Poor Fair Good
Excellent
1. Demonstrates drawings clearly and accurately 0 1 2 3
2. Completely answers guide questions 0 1 2 3
3. Accomplishes and submits laboratory manual on time 0 1 2 3
4. Participates actively in class discussion and activities 0 1 2 3
5. Answers questions correctly 0 1 2 3

Section Score: __________


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 1: Diphyllobothriasis
A nine-year-old pale Filipino girl was brought to the provincial hospital with complaints of vomiting and
loose stools that had started three days earlier. The vomit did not have a foul smell and contained no
blood or mucus, but it did contain undigested food particles. She has a history of recurrent abdominal pain
but was often treated by a traditional healer. Her diet is mostly composed of fresh fruits and fresh water
fish.

Draw and label the life cycle

Guide questions

1. Discuss the presence of anemia in this patient.

2. What are the first and the second intermediate hosts of Diphyllobothrium latum?

3. What is the proper management and control of this condition?

4. How is this condition diagnosed?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 2: Taeniasis/cysticercosis
A 45-year-old man who works in a pork processing plant presents to his physician with new-onset
seizures and bilateral lower extremity weakness. CT of the head reveals several calcified regions and
cystic masses but no solid mass lesion or evidence of bleeding. A complete blood count reveals mild
anemia and a WBC count of 55,000/mm3 with 15% eosinophils.

Draw and label the life cycle

Guide questions

1. What are the infective stages of Taenia solium and Taenia saginata that occurs in man?

2. How is taeniasis diagnosed? How is neurocysticercosis diagnosed?

3. How is this properly managed and controlled?

4. Differentiate morphologically T. solium from T. saginata.


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 3: Hymenolepiasis
A 24-year-old man sought medical attention from his health care provider for intermittent abdominal
cramping and mild diarrhea. A stool specimen was collected in 10% formalin. A formalin-ethyl acetate
concentration was performed and shoed a spherical egg with an inner membrane having two (2) polar
thickening from which arise 4-6 slender polar filaments. The inner membrane enclosed an embryo with 3
pairs of hooklets.

Draw and label the life cycle

Guide questions

1. Differentiate morphologically Hymenolepis nana from Hymenolepis diminuta.

2. What are the common hosts of H. nana and H. diminuta?

3. What is the proper management and prevention of this condition?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 4:Diphylidiasis
A worried mother of an 8-month-old male child sought consult because of white objects found in the
diaper of her child. On examination, it resembles rice particles and are noticeably coming out of the anus
of the child. The family has a number of pet dogs which often interacts with their child. The sample was
sent to the laboratory for microscopic analysis.

Draw and label the life cycle

Guide questions

1. Describe the gravid proglottid and egg pockets often associated with this specie.

2. What type of host is man?

3. What is the appropriate management of this condition?

4. How are they acquired by dogs?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 5: Hydatid disease


A 35-year-old man presents to clinic with abdominal pain, a low-grade fever, and a sensation of
abdominal fullness. He says the symptoms have been going on for some time and have been gradually
worsening. On physical examination she appears jaundiced with notable scleral icterus. X-ray of the
abdomen shows calcified cysts in the right upper quadrant.

Draw and label the life cycle

Guide questions

1. How does man get this parasitic infection?

2. What are usual sources of infection?

3. What other diagnostic method may be used besides radiography?

4. Enumerate the possible definitive hosts.

5. What will happen if the cyst is accidentally ruptured?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Trematodes
Learning Objectives
1. To discuss characteristics and life cycle of trematodes
2. To discuss pathogenesis and clinical manifestations caused by medically important
trematodes
3. To discuss the diagnostic techniques utilized in the isolation and identification of
trematodes
4. To discuss the treatment modalities and management of parasitic trematodes infections
5. To discuss the prevention and control of trematodes infections
6. To apply the concepts learned in this activity in theoretical situations.

Guide questions
1. What are the general and specie specific characteristics of each trematode?
2. What are the common and specie specific clinical manifestations of diseases caused by
trematodes? Explain the pathogenesis.
3. What are the diagnostic modalities and criteria required in the evaluation trematode
infections?
4. What are the treatment and management options in handling trematode infection?
5. How are trematodes prevented and controlled?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

LABORATORY EXERCISES FOR TREMATODES

NAME: _________________________________________________YEAR/SECTION________

Learning Objectives

At the end of the activity, the student shall be able to:

1. Correlate the clinical history, physical examination, diagnostic tests and gross and microscopic
characteristics in the diagnosis of the given cases.

2. Using prepared slides/photographs/gross specimens, recognize following conditions:


a. Sheep liver rot
b. Clonorchiasis/Opisthorchiasis
c. Fasciolopsiasis
d. Echinostomiasis
e. Heterophysiasis
f. Paragonimiasis
g. Schistosomiasis

3. Explain the pathogenesis and symptomatology in the clinical cases.

Evaluation

Criteria: The student


Poor Fair Good Excellent
1. Demonstrates drawings clearly and accurately 0 1 2 3
2. Completely answers guide questions 0 1 2 3
3. Accomplishes and submits laboratory manual on time 0 1 2 3
4. Participates actively in class discussion and activities 0 1 2 3
5. Answers questions correctly 0 1 2 3

Section Score: __________


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 1: Sheep liver rot


A 38-year-old man presented with abdominal pain associated with fever, nausea and vomiting. A
maculopapular rash was also noted on the trunk and arms. There is mild yellowish discoloration of his
conjunctiva. History reveals that he recently went camping and stumbled upon wild watercress which he
consumed. Abdominal ultrasound findings show suspicious lesions in the hepatobiliary tract.

Draw and label the life cycle

Guide questions

1. Identify the 1st an 2nd intermediate host of this condition.

2. How does this parasite cause disease in humans?

3. Discuss preventive measures to control spread of infection.


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 2: Clonochiasis/Opisthorchiasis
A 38-year-old man with fever and yellowish discoloration sought consult at the emergency room. The
patient was brought in by his wife after she found him disoriented and sick in bed. Vital signs are notable
for a temperature of 38°C, heart rate of 100/min, blood pressure of 110/60 mm Hg, and respiratory rate of
24/min. Physical examination reveals tenderness and guarding in the right upper quadrant (RUQ).

Draw and label the life cycle

Guide questions

1. Differentiate Clonorchis sinensis from Opisthorcis felinues based on adult and larval forms, eggs
intermediate hosts.

2. List down the complications that may arise from this parasitic infection.

3. What are the preventive measure against this parasite?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 3: Fasciolopsiasis
A 40 year-old female farmer patient complained of crampy abdominal pain and fever for a duration of
two weeks, associated with loss of appetite and loss of weight. Laboratory findings show leukocytosis
with elevated eosinophils. Urinalysis shows increased pus cells. Incidentally stool examination was
positive for the eggs of Fasciolopsis buski.

Draw and label the life cycle

Guide questions

1. Explain the pathophysiology of the presentation of the patient.

2. What are the 1st and 2nd intermediate hosts?

3. How is this parasitic infection properly treated and controlled?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 4: Echinostomiasis
A 10-year-old boy presented with vomiting, diarrhea and swelling of the feet for six days. The child is
fairly nourished with mild pallor and pedal edema. There was mild distension of abdomen. The liver was
enlarged 2 cm below costal margin, non tender with smooth surface. A few hours after admission, the
child vomited reddish grey, small leaf like worms.

Draw and label the life cycle

Guide questions

1. What is the intermediate host of Echinostoma?

2. What is the morphological characteristic of the egg that will differentiate it from the other intestinal
flukes

3. What is the proper treatment and prevention of this parasitic infection?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 5: Heterophysiasis
A 5-year-old female child recently came back from southeast Asia and was suffering for repeated bouts of
diarrhea. She was treated empirically with metronidazole pending laboratory stool analysis of all possible
parasitic infection. Small operculated oval eggs were recovered from her stool.

Draw and label the life cycle

Guide questions

1. What is the most common Heterophyid in southeast Asia?

2. Differentiate the adult Heterophyid flukes.

3. Discuss the prevention and treatment of this parasitic infection.


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 6: Paragonimiasis
A 21-year-old male presented with cough of more than 2 weeks with occasional blood tinged sputum. He
immediately tested for tuberculosis which show negative results. Due to the persistence of cough with
increasing episodes of hemoptysis, he was then advised to undergo bronchoalveolar lavage to sample his
respiratory tree. Small ovoid eggs with flattened operculum and thickened abopercular ends were noted.

Draw and label the life cycle

Guide questions

1. Give the 1st and 2nd intermediate host of Paragonimus westermani

2. Besides pulmonary paragonomiasis, what other clinical presentations are possible?

3. What is the proper management and prevention of this parasitic infection?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 7: Schistosomiasis
A 19-year-old college student presents to the clinic with fever, hives, headache, weight loss, and cough.
On review of systems, she reports doing field research in Samar over the summer. He recalls an intense
itching sensation while collecting samples in a river. Physical examination reveals lymphadenopathy and
hepatosplenomegaly.

Draw and label the life cycle

Guide questions

1. Differentiate S. japonicum, S.mansoni, and S. haematobium based on egg morphology, clinical


manifestation, complication and location in the body.

2. What is the circumoval precipitin test?

3. What is the management and prevention for this parasitic infection.


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Arthropods
Learning Objectives
1. To discuss characteristics and life cycle of medically important arthropods
2. To discuss pathogenesis and clinical manifestations caused by arthropods
3. To discuss the diagnostic techniques utilized in the isolation and identification of
arthropods
4. To discuss the treatment modalities and management of parasitic arthropods
5. To discuss the prevention and control of arthropods
6. To apply the concepts learned in this activity in theoretical situations.

Guide questions
1. What are the general and specie specific characteristics of each arthropod?
2. What are the common and specie specific clinical manifestations of diseases caused by
arthropods? Explain the pathogenesis.
3. What are the diagnostic modalities and criteria required in the evaluation arthropod infections?
4. What are the treatment and management options in handling arthropod infection?
5. How are arthropods prevented and controlled?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

LABORATORY EXERCISES FOR ARTHROPODS

NAME: _________________________________________________YEAR/SECTION________

Learning Objectives

At the end of the activity, the student shall be able to:

1. Correlate the clinical history, physical examination, diagnostic tests and gross and microscopic
characteristics in the diagnosis of the given cases.

2. Using prepared slides/photographs/gross specimens, recognize following conditions:


a. Scabies
b. Pediculosis
c.

3. Explain the pathogenesis and symptomatology in the clinical cases.

Evaluation

Criteria: The student


Poor Fair Good Excellent
1. Demonstrates drawings clearly and accurately 0 1 2 3
2. Completely answers guide questions 0 1 2 3
3. Accomplishes and submits laboratory manual on time 0 1 2 3
4. Participates actively in class discussion and activities 0 1 2 3
5. Answers questions correctly 0 1 2 3

Section Score: __________


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 1: Scabies
65-year-old nursing home resident presents with a 1-month history of intensely pruritic, erythematous
papules on his torso, groin, and extremities. The pruritus is worse at night. He was treated with
prednisone and loratadine without improvement.

Draw and label the life cycle

Guide questions

1. How does the ectoparasite cause clinical manifestations?

2. How is this appropriately managed and prevented from spreading?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Case 2: Pediculosis
A 75-year old homeless man complains of an itchy scalp. On examination of the pruritic area, it is
almost devoid of hair and has abrasions. Close inspection show numerous nits along the hair shafts.

Draw and label the life cycle

Guide questions

1. How does the ectoparasite cause clinical manifestations?

2. What is the appropriate management and prevention against this ectoparasite?


Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Parasitic Zoonoses

Learning Objectives
1. To define the zoonoses and its classification
2. To determine the impact of zoonoses on health and economy
3. To explain the factors that contribute to the propagation of zoonotic parasites
4. To discuss the importance of zoonoses as a public health indicator
5. To apply the concepts learned in this activity in theoretical situations

Guide questions
1. Define zooinoeee.
2. What are the difereint typee of zooinoeee?
3. What ie the impact of zooinoeee oin health aind the ecoinomy?
3. What are the factore that cointribute to propagatoin of zooinoeee?
4. How doee paraeitc zooinoeee iinfueince public health programe?
5. What are the curreint programe of the goverinmeint that target paraeitc zooinoeee iin our couintry?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Immunocompromised Host and Parasitic Infections


Learning Objectives
1. To discuss the susceptibility of immunocompromised individuals to parasitic infection
2. To discuss the medically important parasitic infections in the immunocompromised
3. What are the diagnostic methods used in the assessment of immunocompromised with
parasitic infections
4. To apply the concepts learned in this activity in theoretical situations

Guide questions
1. What are the components of the immune system tat deal with parasitic infections?
2. What are the risk factors increase the susceptibility of immunocompromised to parasitic
infections?
3. Discuss the etiology and pathogenesis of protozoans and helminths of special importance to
immunocompromised individuals.
4. What are the medically important parasites that infect immunocompromised individuals?
Include also nosocomial parasitic infections.
5. What are the diagnostic methods used in the assessment of immunocompromised individuals
to determine parasitism?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Changing Environment and Disease Patterns


Learning Objectives
1. To discuss the epidemiology of parasitic infectious diseases
2. To explain the effects of a changing environment on disease patterns of parasitic infections
3. To recognize interdisciplinary, national and transnational approach in strategic
management of parasitic infections
4. To apply the concepts learned in this activity in theoretical situations

Guide questions
1. What is the prevalence/incidence of parasitic infections? Locally and internationally.
2. What factors affect the distribution of parasitic infections?
3. What are the methods that have contributed to failure/control of health programs that target
parasitic infections?
4. How does a changing environment influence the distribution of parasitic infections?
5. Recall the economic impact of parasitic infections to humans and livestock.
6. What local and international agencies have committed themselves in the control of parasitism
in the community?
Emilio Aguinaldo Educational Corporation
School of Medicine
Department of Parasitology

Name: _______________________________ Date: ______________ Signature: ____________

Case Reporting, Institutional Partners in Parasitic Infection Control and Management


Learning Objectives
1. To discuss the role of local and international institutions in parasitic control and
management
2. To present and interesting case of parasitic infection to the group

Guide questions
1. What is the significance of international and local statistics of parasitic infections?
2. What is the role of local and international institutions in the control of management of
parasitic infections?
3. How are suspected uncommon parasitic infections referred to local and international
institutions?

You might also like