Chapter 9&10: Microscopic Examination of Urinary Sediment: Casts

Chapter 9&10:
Microscopic Examination
of Urinary Sediment: Casts
Graff’s Textbook of Urinalysis and Body Fluids,

Third Edition
Mundt, L. & Shanahan K.
Copyright © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins

Chapters Outline: Casts
• Overview of Urinary Cast Formation

• Hyaline Casts
• Cellular Casts
• Granular Casts
• Waxy Casts
• Pseudo Casts
Copyright © 2016 Wolters Kluwer • All Rights Reserved

Urinary Cast: Formation
• Formed in lumen of the kidney distal convoluted tubules

and Loop of Henle.
• Molded in the shape of the tubules
• Tamm–Horsfall mucoprotein comprises the matrix
• May contain cells that are present in the tubules at the
time of cast formation.

Urinary Cast: Formation (cont.)
• Factors of cast formation:

– urinary stasis
– Acid pH
– high solute concentration
– abnormal ionic or protein constituents
• May be indicated by the presence of urinary protein.
– However, casts can be present in the absence of
protein.
– Microscopic examination is important.

Urinary Cast: Identification of Casts
• Casts:
– have parallel sides with rounded or blunted ends
– vary in size and shape according to the tubules in
which they were formed
– convoluted, straight, or curved
– are cylindrical in shape with no dark edges

Urinary Cast: Significance of Casts
• The width of the cast indicates the diameter of the
tubule in which it was formed
• Broad casts
– formed in pathologically dilated or atrophied
tubules or in collecting tubules
– indicate renal failure
• Cast are always renal in origin and indicate pathologies:

– glomerular damage
– tubular damage
– renal inflammation
– renal infection

Urinary Cast: Broad and Narrow
Fine granular casts and WBCs

Urinary Cast: Classification
• Based on appearance and contents:

– hyaline
– red cell
– white cell
– epithelial cell
– granular (coarse and fine)
– waxy
– fatty
– mixed

Urinary Cast: Sequence of Degeneration
Sequence of urinary cast degeneration

Urinary Cast: Enumeration
• Counted on low-power (100x)
– More frequently seen along the edges of coverslip
• Reported according to type and range seen
– 0-2, 2-5, 5-10, 10-20
• Example:
– Hyaline casts 5-10/LPF
– Granular casts 2-5/LPF

Urinary Casts: Hyaline Casts
Hyaline cast and red blood cells.

Urinary Casts: Hyaline Casts (cont.)
• Can be seen in even the mildest kind of renal disease.

• Not associated with any one disease in particular.
• A few hyaline casts may be found in the normal urine.
• Frequently present:
– following physical exercise
– physiologic dehydration


Hyaline casts. Viewed with an 80A filter

• Hyaline casts using phase contrast microscopy

Urinary Casts: Red Blood Cell Casts
(cont.)
Red cell cast and RBCs

(cont.)
• Always pathologic
• Hematuria is present
• Conditions include:
– acute glomerulonephritis
– lupus nephritis
– Goodpasture syndrome
– subacute bacterial endocarditis
– renal trauma
– renal infarction
– severe pyelonephritis
– right-sided congestive heart failure
– renal vein thrombosis
– periarteritis nodosa

(cont.)
Convoluted red blood cell cast

(cont.)

Urinary Casts: White Blood Cell Casts
(cont.)
White cell cast and WBCs

(cont.)
• Can be present during renal infection and inflammation.
– acute pyelonephritis
– interstitial nephritis
– lupus nephritis
– glomerular disease

(cont.)

(cont.)
SM-stained WBC cast

Urinary Casts: Epithelial Cell Casts

(cont.)
• May be present after exposure to:
– nephrotoxic agents
– viruses (e.g., cytomegalovirus, hepatitis virus)
• Due to damage that accompanies glomerular injury
• Also present in the rejection of a kidney allograft

(cont.)

Urinary Casts: Mixed Cell Casts
Mixed cell cast, WBCs, and RBCs.

Urinary Casts: Mixed Cell Casts (cont.)
SM-stained mixed cellular cast.

Urinary Casts: Mixed Cell Casts (cont.)
SM-stained hyaline cast, granular cast, mixed

cellular cast, and partially degenerated renal
tubule epithelial cells

Urinary Casts: Granular Casts
Broad coarsely granular
Finely granular casts

Urinary Casts: Granular Casts (cont.)
• Usually indicate renal disease.
• But may be briefly present after strenuous exercise.
• These casts have remained in the tubule for enough
time to allow for the degeneration of cellular
components.
• May be confused with casts containing bacteria or
crystals.

Coarse granular cast
Coarse granular cast

Bilirubin-stained granular cast
SM-stained granular cast

Urinary Casts: Waxy Casts
• Waxy cast and WBCs
Waxy cast, WBCs, and

bacteria

Urinary Casts: Waxy Casts (cont.)
• Found in urine from patients with:
– severe chronic renal failure
– malignant hypertension
– renal amyloidosis
– and diabetic nephropathy
– acute renal disease
– tubular inflammation and degeneration
– and during renal allograft rejection.

Urinary Casts: Waxy Casts (cont.)
• Waxy cast and amorphous urates.

Urinary Casts: Granular to Waxy
transformation
Fine granular cast becoming a waxy cast.

Urinary Casts: Waxy Casts
Convoluted waxy cast.
SM-stained waxy granular cast.

Urinary Casts: Fatty Casts

Urinary Casts: Fatty Casts (cont.)
• Present during fatty degeneration of the RTE cells
– degenerative tubular disease
– nephrotic syndrome
– diabetic glomerulosclerosis
– lipoid nephrosischronic
– Glomerulonephritis
– Kimmelstiel–Wilson syndrome
– Systemic Lupus Erythematosis
– toxic renal poisoning

Urinary Casts: Other Casts
• Mixed cast.

Urinary Casts: Pseudo Casts
Cylindroid.

Urinary Casts: Confusing Artefacts-
Mucous Threads

Urinary Casts: Confusing Artefacts-Fibers
Fibers
Debris from a diaper

Urinary Casts: Confusing Artefacts-Hair
Hair and a coarsely granular cast

Urinary Casts: Confusing Sediment - Yeast
SM-stained yeast with pseudohyphae and WBCs

Urinary Casts: Confusing Comparison
A. Fine granular cast B. Fiber.
Copyright © 2016 Wolters Kluwer • All Rights Reserved Copyright © 2016 Wolters Kluwer • All Rights Reserved

Summary
• Casts, comprised of a Tamm–Horsfall mucoprotein
matrix are formed in lumen of distal tubules.
• Casts are classified by their contents.
• Several artifacts may be misidentified as casts.
• Reagent strip tests for protein may or may not be
positive when casts are present.
• Casts may be present in physiologically normal
conditions.
• Casts usually accompany renal disorders.

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Chapter 9&10:

Graff’s Textbook of Urinalysis and Body Fluids,

Copyright © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins

• Overview of Urinary Cast Formation

Copyright © 2016 Wolters Kluwer • All Rights Reserved

• Formed in lumen of the kidney distal convoluted tubules

Copyright © 2016 Wolters Kluwer • All Rights Reserved

• Factors of cast formation:

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

• Cast are always renal in origin and indicate pathologies:

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Fine granular casts and WBCs

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

• Based on appearance and contents:

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Hyaline cast and red blood cells.

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

• Can be seen in even the mildest kind of renal disease.

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Hyaline casts. Viewed with an 80A filter

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

White cell cast and WBCs

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SM-stained WBC cast

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Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved

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Copyright © 2016 Wolters Kluwer • All Rights Reserved

Mixed cell cast, WBCs, and RBCs.

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Copyright © 2016 Wolters Kluwer • All Rights Reserved

SM-stained mixed cellular cast.

Copyright © 2016 Wolters Kluwer • All Rights Reserved

Copyright © 2016 Wolters Kluwer • All Rights Reserved