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    CHO Cell Line From Chinese Hamster Ovary: Properties

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    Ahnaph Rafin

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    © All Rights Reserved
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    CHO Cell Line From Chinese Hamster Ovary: Properties

    Uploaded by

    Ahnaph Rafin
    108 views2 pages

    Original Title

    CHO Cell line

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    Copyright:

    © All Rights Reserved
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    108 views2 pages

    CHO Cell Line From Chinese Hamster Ovary: Properties

    Uploaded by

    Ahnaph Rafin

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    © All Rights Reserved
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    of 2

    3/12/2020 CHO Cell Line from Chinese hamster ovary from hamster ovary, 85050302 | Sigma-Aldrich

    Bangladesh Home CHO Cell Line from Chinese hamster ovary

    Sigma

    CHO Cell Line from Chinese hamster ovary


    from hamster ovary, 85050302
    Synonym: Chinese hamster ovary cell line

    SKU-Pack Size

    85050302-DNA-5UG

    85050302-RNA-5UG

    85050302-1VL

     
    CHO-K1 Cell Line from CHO Protein-Free Cell CHO-K1.Cl-0204 CHO/dhFr-AC-free cell line CHO/dhFr-
    hamster Line from Chinese ... 15042903, from hamster ovary 05011002 94060607
    from hamster ovary, 85051005 00102307

    Properties
    Related Categories A-C, Cell Culture, ECACC Cell Lines More...

    biological source hamster ovary

    availability not available in Japan

    growth mode Adherent

    karyotype Hypodiploid, modal no. 20

    morphology Epithelial

    products Not specified

    receptors Not specified

    Show More (11)

    Description
    Cell Line Origin
    Hamster Chinese ovary

    Cell Line Description


    A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

    Application
    CHO Cell Line from Chinese hamster ovary has been used:
    • to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
    • to carry out the vaginolysin (VLY) cytolytic activity assay
    • as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

    Nutritional and gene expression studies

    Culture Medium
    Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

    1/3
    3/12/2020 CHO Cell Line from Chinese hamster ovary from hamster ovary, 85050302 | Sigma-Aldrich

    Subculture Routine
    Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

    Other Notes
    Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the
    depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the
    potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

    Safety Information Documents


    RIDADR NONH for all modes of transport Certificate of Analysis (COA)

    WGK Germany 3 Enter Lot Number

    How to enter a Lot Number

    Bulk Quote-Order Product

    Frequently Asked Questions

    Which document(s) contains shelf-life or expiration date information for a given product?
    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    How do I get lot-specific information or a Certificate of Analysis?


    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    How do I find price and availability?


    There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can
    contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.

    My question is not addressed here, how can I contact Technical Service for assistance?
    Ask a Scientist here.

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    150 Kunitz units/mg solid

    https://www.sigmaaldrich.com/catalog/product/sigma/cb_85050302?lang=en&region=BD 2/3

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