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    2HPLC5667

    Uploaded by

    Jany S
    The document defines key terms and concepts in high performance liquid chromatography (HPLC). It states that HPLC stands for both high pressure liquid chromatography and high performance liquid chromatography. It describes different types of HPLC methods, including liquid/liquid chromatography, liquid/solid chromatography, and ion exchange and size exclusion chromatography. It also discusses column efficiency, eluent strength, isocratic and gradient elution, normal and reversed phase chromatography, and advantages of HPLC over traditional low pressure liquid chromatography.

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    2HPLC5667

    Uploaded by

    Jany S
    74 views4 pages
    The document defines key terms and concepts in high performance liquid chromatography (HPLC). It states that HPLC stands for both high pressure liquid chromatography and high performance liquid chromatography. It describes different types of HPLC methods, including liquid/liquid chromatography, liquid/solid chromatography, and ion exchange and size exclusion chromatography. It also discusses column efficiency, eluent strength, isocratic and gradient elution, normal and reversed phase chromatography, and advantages of HPLC over traditional low pressure liquid chromatography.

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    The document defines key terms and concepts in high performance liquid chromatography (HPLC). It states that HPLC stands for both high pressure liquid chromatography and high performance liquid chromatography. It describes different types of HPLC methods, including liquid/liquid chromatography, liquid/solid chromatography, and ion exchange and size exclusion chromatography. It also discusses column efficiency, eluent strength, isocratic and gradient elution, normal and reversed phase chromatography, and advantages of HPLC over traditional low pressure liquid chromatography.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    74 views4 pages

    2HPLC5667

    Uploaded by

    Jany S
    The document defines key terms and concepts in high performance liquid chromatography (HPLC). It states that HPLC stands for both high pressure liquid chromatography and high performance liquid chromatography. It describes different types of HPLC methods, including liquid/liquid chromatography, liquid/solid chromatography, and ion exchange and size exclusion chromatography. It also discusses column efficiency, eluent strength, isocratic and gradient elution, normal and reversed phase chromatography, and advantages of HPLC over traditional low pressure liquid chromatography.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 4

    1.

     HPLC stands for


    A.High Pressure Liquid Chromatography
    B.High Performance Liquid Chromatography
    C.both (a) and (b)
    D
    Highly Placed Liquid Chromatography
    .

    2. The eluent strength is a measure of


    A.solvent adsorption energy
    B.solvent absorption energy
    C.solvent diffusivity
    D
    solvent mixing index
    .

    3. HPLC methods include


    A.liquid/liquid (partition) chromatography
    B.liquid/solid (adsorption) chromatography
    C.ion exchange and size exclusion chromatography
    D
    all of the above
    .

    4. Column efficiency is measured in terms of number of plates which is


    A.inversely related to the square of the peak width
    B.directly related to the square of the peak width
    C.inversely related to the cube root of the peak width
    D
    directly related to the square of the peak width
    .
    5. For a typical adsorbent such as silica gel, the most popular pore diameters are
    A.10 and 50 A°
    B.60 and 100 A°
    C.100 and 150 A°
    D
    150 and 200 A°
    .
    6. An isocratic elution in HPLC is one in which the composition of the solvent

    A. remains constant

    B. changes continuously

    C. Changes in a series of steps

    D. none of these

    7. In reversed phase HPLC, there is a

    A. non polar solvent/polar column

    B. polar solvent/non-polar column

    C. non polar solvent/non-polar column

    D. any of the above

    8. Which of the following statements is true for a refractive index detector in HPLC?

    A. It is more sensitive than a UV detector

    B. It can only be used for isocratic elutions

    C. It does not respond to many solutes

    D. none of above

    9. A gradient elution in HPLC is one in which the composition of the solvent

    A. remains constant

    B. is changed continuously or in a series of steps


    C. both (a) and (b)
    D. none of the above

    10. An eluotropic series

    A. ranks solvents by their relative abilities to displace solutes from a given absorbent
    B. ranks column packing material by their relative abilities to retain solutes on the column

    C. is a measure of the solvent adsorption energy

    D. none of the above

    11. Dwell volume is defined as

    A. the volume of solvent contained in a liquid chromatographic column

    B. the time required for the gradient to reach the column

    C. the volume of the column between the point at which solvents are mixed and the
    beginning of the column
    D. none of these

    12. Void volume refers to the

    A. total volume of eluent in the column the remainder being taken up by the packing material

    B. the volume of solvent contained in a liquid chromatographic column

    C. the time required for the gradient to reach the column

    D. the volume of the column between the point at which solvents are mixed and the beginning of
    the column

    13. In normal phase HPLC, there is a

    A. non polar solvent/polar column

    B. polar solvent/non-polar column

    C. non polar solvent/non-polar column

    D. any of the above

    14. Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid
    chromatography)?

    A. Greater sensitivity and reusable columns


    B. Ideal for ionic species and large molecules

    C. Sample recovery

    D. All of the above

    15. Which can be used as a mobile phase in HPLC applications?

    A. Any compound with solubility in liquid

    B. Any compound with limited solubility in liquid

    C. Any compound with non-solubility in liquid

    D. Any of the above

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