Atcc Bacterial Culture Guide: Tips and Techniques For Culturing Bacteria and Bacteriophages
Atcc Bacterial Culture Guide: Tips and Techniques For Culturing Bacteria and Bacteriophages
Atcc Bacterial Culture Guide: Tips and Techniques For Culturing Bacteria and Bacteriophages
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Table of Contents
This guide contains general technical information for bacterial growth, propagation, preservation, and
application. Additional information on bacterial culturing can be found in Bergey’s Manual of Systematic
Bacteriology, 2nd Edition, published by Springer, New York1.
Product Sheet
ATCC bacterial strains are shipped with a product sheet that contains detailed information on the
rehydration and expansion of materials, as well as ideal growth and propagation conditions. This product
sheet, as well as additional information, can be found on the ATCC website or can be requested from the
ATCC Technical Service Department.
Nomenclature Changes
Changes in taxonomy or further analysis of bacterial strains may lead to a change in nomenclature. These
changes are indicated on product sheets and can be found online on the product page. Further information
on bacterial nomenclature changes can be found on the List of Prokaryotic Names with Standing in
Nomenclature (LPSN), which can be accessed online at http://www.bacterio.cict.fr/.
Preparation of Medium
In advance, prepare the appropriate medium and additional reagents necessary for bacterial strain revival
and growth. Ensure that the proper incubation conditions are ready. Information for the formulation and
preparation of the media and incubation conditions for these products is available on the ATCC website.
Tip
Insulator
Cotton plug
Outer vial (soft glass) Borosilicate glass
Inner vial
Freeze-dried pellet Freeze-dried cells
Cotton
Desiccant with indicator
Wrap gauze around the ampule, and break at the scored area.
Care should be taken not to have the gauze too wet, or
alcohol could be sucked into the culture when the vacuum is
broken. Rehydrate material at once.