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Digestive Tract

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Stool examination is a series of tests done on a stool sample to help diagnose certain conditions

affecting the digestive tract. This includes infection, malabsorption, and gastrointestinal


bleeding.
Before the test, the patient should avoid medicines that might alter the results. This includes
antacids, antidiarrhea and antibiotic medication. If the stool is being tested for blood, certain
foods might also be avoided such as red meats and carrots.
For the collection, the patient is asked to collect the stool in a clean, wide mouth container. The
stool should be uncontaminated with urine or any other body secretions that might affect the
result of the test. More than 2 grams of stool is required for the stool examination and the
container should be labeled with the name of the patient and the date it was collected.
The stool is then sent to the laboratory for examination.
The stool examination is divided into three parts which is the gross examination, microscopic
examination and chemical examination.
In gross examination, you have to check for the consistency of the stool, such as if it is loosely
formed stools, watery stools as seen in diarrhea, dry or hard stools as seen in constipations, pasty
stool that are due to high fat content and ribbon like stool as seen in rectal narrowing and partial
obstruction.
The color of stools are brown which is normal and is due to stercobilinogen, yellow or yellow
green which is seen in diarrhea, black and tarry stools which may indicate bleeding from upper
GI tract, reddish color which may indicate bleeding in lower GI tract, clay colored stools that
might indicate biliary obstruction and pale color stool that might indicate malabsorption.
You also have to check for the quantity of the stool, the normal quantity of stool is 100 to 200
grams per day. Disorders with poor food breakdown and absorption might lead to large bulky
frothy and foul smelling stools.
Next is the odour, the normal odor of the stool is due to indole and skatole which are formed by
bacterial fermentation and putrefaction. The foul odor is due to undigested protein and excessive
intake of carbohydrates and sickly odor is due to undigested lactose and fatty acids.
Lastly is the mucous, it is a translucent gelatinous material in the surface of the stool. It can be
seen in severe constipation and mucous colitis. While mucus and blood are seen in patients with
ulcerative colitis, amoebiasis and etc.
Next is microscopic examination, it is done to detect the presence of leukocytes, RBC,
macrophages, fats, ova, cyst and other microorganisms.
Normal stools may contain occasional white blood cells. To look for WBC, the smears must be
prepared from the area of mucus or from watery stools. Increase number of WBC is associated
with shigellosis, clostridium difficile, salmonella and campylobacter infection.
RBC in stool might indicate bleeding in upper or lower GI as evidence by either bright red
bleeding or black tarry stool.
Presence of macrophages in the stool is indicative of dysentery and ulcerative colitis.
Presence of fat in the stool might be cause by malabsorption, deficiency of pancreatic digestive
enzyme or deficiency of bile.
Stool pH depends on the dietary intake and bacterial fermentation in the small intestine. Alkaline
stool might be seen in patients with colitis, diarrhea and antibiotic therapy while acidic stool is
seen on disaccharide deficiency, fat and carbohydrate malabsorption. The reducing substance in
the stool is important in infants with chronic diarrhea to rule out lactose intolerance.12

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