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    Dna Extraction Simulation

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    Pamela Noel Martinez
    This document provides an overview of DNA extraction. It describes the phenol/chloroform method to extract cellular DNA. The process involves three stages: cell dissolution to break open cells and nuclei, precipitation to remove proteins and impurities, and purification to isolate pure DNA. DNA extraction is a fundamental technique used in research labs to study DNA and its relation to diseases, develop vaccines and enzymes, and for forensic analysis.

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    © All Rights Reserved
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    Dna Extraction Simulation

    Uploaded by

    Pamela Noel Martinez
    42 views1 page
    This document provides an overview of DNA extraction. It describes the phenol/chloroform method to extract cellular DNA. The process involves three stages: cell dissolution to break open cells and nuclei, precipitation to remove proteins and impurities, and purification to isolate pure DNA. DNA extraction is a fundamental technique used in research labs to study DNA and its relation to diseases, develop vaccines and enzymes, and for forensic analysis.

    Original Description:

    Principios de la extracción de ADN

    Original Title

    dna-extraction-simulation

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    This document provides an overview of DNA extraction. It describes the phenol/chloroform method to extract cellular DNA. The process involves three stages: cell dissolution to break open cells and nuclei, precipitation to remove proteins and impurities, and purification to isolate pure DNA. DNA extraction is a fundamental technique used in research labs to study DNA and its relation to diseases, develop vaccines and enzymes, and for forensic analysis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    42 views1 page

    Dna Extraction Simulation

    Uploaded by

    Pamela Noel Martinez
    This document provides an overview of DNA extraction. It describes the phenol/chloroform method to extract cellular DNA. The process involves three stages: cell dissolution to break open cells and nuclei, precipitation to remove proteins and impurities, and purification to isolate pure DNA. DNA extraction is a fundamental technique used in research labs to study DNA and its relation to diseases, develop vaccines and enzymes, and for forensic analysis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Biology Molecular Biology

    DNA Extraction

    General Aim Theoretical Background/Context (Cont’)


    To extract cellular DNA using phenol/chloroform DNA has been the target of a lot of research.
    method. Investigation of the DNA structure and sequence
    in relation to diseases helped in finding out the
    Method molecular basis and cure for various diseases.
    Phenol/chloroform method. DNA study also allowed the production of many
    vaccines, hormones and enzymes. DNA study was
    Learning Objectives (ILOs) also very beneficial in the forensic/medico legal
    Demonstrate proficiency with the protocol involved entities. To study DNA it must be extracted out of
    in DNA extraction. the cell, Hence; DNA extraction technique is
    Identify the role of specific reagents and equipment widely used in research labs.
    in the extraction of DNA. The isolation of the DNA is done in stages:
    Practice basic laboratory techniques.
    1. Cell dissolution: In this stage the cell and the
    Conclude downstream applications of DNA
    nucleus are broken to extract a DNA sample using
    extraction.
    a lysis buffer.
    2. Precipitation: In this stage, proteins and impurities
    Theoretical Background/Context are removed from the sample.
    3. Purification: This stage involves isolating the DNA
    DNA (deoxyribonucleic acid) is a complex molecule
    completely from the other substances, for a final
    that contains the genetic material of a cell. It defines
    eluted pure DNA sample preserved in a buffer.
    the structure and the functions of a living organism.
    DNA is also responsible for heredity. Principle of Work
    DNA is composed of a series of nucleotides linked The extraction of DNA involves lysis of cellular and
    together. These nucleotides are basically made of a nuclear membranes to extract DNA from within. This
    nitrogenous base, a molecule of sugar (deoxyribose) is followed by separation of DNA from impurities,
    and a phosphate group. The sequence of nucleotides proteins, and other substances. DNA extraction
    in the DNA molecule determines the hereditary traits usually proceeds through three stages:
    of a cell. Nucleotides are packed to fit inside the cell.
    1. Cell dissolution: where the cell and the nucleus are
    DNA takes the shape of a double helix, which, in
    broken to extract DNA into the buffer.
    addition to the DNA molecule contains bound
    proteins. DNA double helix is enclosed inside a cell 2. Precipitation: where proteins and impurities
    membrane in case the cell doesn’t have a nucleus like are removed from the sample.
    in prokaryotes, and inside the nuclear membrane in 3. Purification: This final stage involves further
    cells with nuclei like in eukaryotes. purification of DNA to get a completely pure
    DNA sample ready to be used for downstream
    applications.

    www.praxilabs.com

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