WST-1 Assay Kit (Cell Proliferation) Ab65475 WST-1 Assay Kit (Cell Proliferation) Ab65475
WST-1 Assay Kit (Cell Proliferation) Ab65475 WST-1 Assay Kit (Cell Proliferation) Ab65475
WST-1 Assay Kit (Cell Proliferation) Ab65475 WST-1 Assay Kit (Cell Proliferation) Ab65475
Overview
The WST-1 assay provides by far the easiest and most sensitive means for performing a
quantitative cell proliferation assay, cell viability assay, or cytotoxicity assay in mammalian cells.
The WST-1 assay protocol is based on the cleavage of the tetrazolium salt to formazan by cellular
mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is
directly proportional to the number of living cells. The formazan dye produced by viable cells can
be quantified by microplate reader by measuring the absorbance of the dye solution at 440 nm.
Notes This kit was previously called Quick Cell Proliferation Assay Kit II, and WST-1 Cell Proliferation
Assay Kit.
The WST-1 assay is add-and-read, and requires no washing, harvesting, or solubilization steps. It
is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates
well with the [3H]-thymidine incorporation assay.
Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell
proliferation assay kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of
products that contain European Authorisation list (Annex XIV) substances.
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It is the responsibility of our customers to check the necessity of application of REACH
Authorisation, and any other relevant authorisations, for their intended uses.
Properties
Relevance Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell
division, resulting in the expansion of a cell population.
Images
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HL60 cell proliferation measured after 48h incubation
(37°C/5%CO2) and 2h WST at various camptothecin
concentrations
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