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    BioGT Lactase Enzyme Activity Virtual Lab

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    anna roth
    The document summarizes a student's investigation of how temperature and pH affect the activity of the lactase enzyme. The student ran simulations varying the temperature from 0 to 80 degrees Celsius and pH from 1 to 13. The data showed that lactase worked best at 40 degrees Celsius and pH of 7, with higher reaction rates. Outside the optimal ranges, the reaction rates decreased or stopped. The student concluded that enzymes have an optimal temperature and pH range for chemical reactions to occur efficiently, and deviations negatively impact the reaction rate.

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    © All Rights Reserved
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    BioGT Lactase Enzyme Activity Virtual Lab

    Uploaded by

    anna roth
    727 views5 pages
    The document summarizes a student's investigation of how temperature and pH affect the activity of the lactase enzyme. The student ran simulations varying the temperature from 0 to 80 degrees Celsius and pH from 1 to 13. The data showed that lactase worked best at 40 degrees Celsius and pH of 7, with higher reaction rates. Outside the optimal ranges, the reaction rates decreased or stopped. The student concluded that enzymes have an optimal temperature and pH range for chemical reactions to occur efficiently, and deviations negatively impact the reaction rate.

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    The document summarizes a student's investigation of how temperature and pH affect the activity of the lactase enzyme. The student ran simulations varying the temperature from 0 to 80 degrees Celsius and pH from 1 to 13. The data showed that lactase worked best at 40 degrees Celsius and pH of 7, with higher reaction rates. Outside the optimal ranges, the reaction rates decreased or stopped. The student concluded that enzymes have an optimal temperature and pH range for chemical reactions to occur efficiently, and deviations negatively impact the reaction rate.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    727 views5 pages

    BioGT Lactase Enzyme Activity Virtual Lab

    Uploaded by

    anna roth
    The document summarizes a student's investigation of how temperature and pH affect the activity of the lactase enzyme. The student ran simulations varying the temperature from 0 to 80 degrees Celsius and pH from 1 to 13. The data showed that lactase worked best at 40 degrees Celsius and pH of 7, with higher reaction rates. Outside the optimal ranges, the reaction rates decreased or stopped. The student concluded that enzymes have an optimal temperature and pH range for chemical reactions to occur efficiently, and deviations negatively impact the reaction rate.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    The text discusses how temperature and pH impact the rate of enzyme reactions, with every enzyme having an optimal temperature and pH range.

    The data shows that the enzyme lactase works best at 40 degrees Celsius, and outside of this optimal temperature the reaction rate decreases significantly.

    The data indicates that lactase works best at a pH of 7, while outside of this pH range the reaction rate is much lower.

    Name: Period: Date:

    Unit 2 Cells and Disease Activity 11 Investigating Enzyme Function

    Lactase Enzyme Activity with Data Analysis


    Simulation at Jon Darkow

    Pre-Lab:
    1. Read the Background section and view the
    diagram to complete the following. Define the
    following and explain how each relates to the
    lactase chemical reaction (see diagram for help
    as well).

    Term Definition and Relation to the Lactase Chemical Reaction

    Enzyme a catalyst that brings a chemical reaction. it digests the lactase

    Substrate a molecule upon which an enzyme acts

    Active Site where the chemical reaction occurs

    Reaction Rate at what speed the chemical reaction occurs

    Activation the energy used to undergo a certain chemical reaction


    Energy

    2. What type of biomolecule is an enzyme?


    proteins

    3. What is the monomer of an enzyme?


    amino acids

    4. What is another name for an enzyme?

    catalyst

    5. How do enzymes speed up chemical reactions?

    they lower the activation energy for reactions

    1
    Name: Period: Date:

    Unit 2 Cells and Disease Activity 11 Investigating Enzyme Function

    Simulation Option 1: Temperature

    Start with the initial lactose at 500 mg/dL and the pH at 7.


    Do not vary these parameters while testing for temperature,
    they are environmental controls. The temperature is in
    degrees Celsius. Vary the temperature by 20oC.

    Run each experiment 3 times (for reliability in the results.)

    ** Click on the 20 minute mark to get your run # see photo

    Temperature Data table:

    0oC 20oC 40oC 60oC 80oC

    Run 1 0 470 493 115 0

    Run 2 0 471 497 140 0

    Run 3 0 472 496 119 0

    Average: 0 471 495 124 0

    Temperature Analysis:

    1. What temperature did the enzyme lactase work best? This is considered the optimal temperature.
    Explain your answer using data from your simulation.

    40 degrees. at that temperature there was the highest data compared to all the other temperatures
    data

    2. Explain what happened to the rate of reaction when lactase was placed out of its optimal temperature.
    Why does this happen?

    the data came out as 0 each run

    2
    Name: Period: Date:

    Unit 2 Cells and Disease Activity 11 Investigating Enzyme Function

    Simulation Option 2: pH
    Start with the initial lactose at 500 mg/dL and the
    temperature at 25 degrees Celsius. Do not vary these
    parameters while testing for pH, they are environmental
    controls.

    Vary by a pH of 3. Run each experiment 3 times (for


    reliability in the results.)

    ** Click on the 20 minute mark to get your run # see photo

    pH Date Table:

    pH 1 4 7 10 13

    Run 1 0 237 489 128 5.82

    Run 2 0 218 496 105 6.05

    Run 3 0 230 494 101 6.42

    Average 0 228 493 111 6

    pH Analysis:

    1. What pH did the enzyme lactase work best? This is considered the optimal pH. Explain your answer
    using data from your simulation.

    a pH of 7 worked best. my answer is proven through my data, it is clear that when the pH was 7 it was
    a lot higher numbers in the data than other pH levels

    2. Explain what happened to the rate of reaction when lactase was placed out of its optimal pH. Why
    does this happen?

    the reaction rate was very low, either 0 or below ten

    3
    Name: Period: Date:

    Unit 2 Cells and Disease Activity 11 Investigating Enzyme Function

    Conclusion: Write a CER paragraph explaining the results. Be sure to include the following in your
    CER.

    ● Summarizes data used to draw conclusions.


    ● Conclusions follow data (no wild guesses or leaps of logic).
    ● Hypothesis is rejected or accepted based on the data.
    ● Discusses possible experimental errors.
    ● Discusses applications or real world connections.

    Claim: The claim should be a complete sentence that answers the question:
    How does temperature and pH affect enzyme controlled reactions?

    there is an optimal zone for pH, and for temperature that affects the enzyme reaction rates.

    Evidence: 
    - You must include 3 pieces of evidence from the Virtual Lab – data that supports your claim!

    in my temperature data, when the temperature was too high or too low the data came out as 0. this
    similarly occurred with my pH data. for both there is a clear optimal zone for the enzyme reaction rates
    to thrive

    Reasoning: 
    -Connect your evidence to your understanding of how enzymes affect the rate of chemical reactions.
    --You need to discuss each piece of evidence. You should not discuss any data that you did not highlight in your
    evidence section.

    4
    Name: Period: Date:

    Unit 2 Cells and Disease Activity 11 Investigating Enzyme Function

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