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    Formaldehyde: Application Note

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    PaoloMM
    Formaldehyde is used for disinfection but is also carcinogenic, so formaldehyde donors are used instead to maintain low free formaldehyde concentrations. Determining free formaldehyde is difficult due to its chemistry and detection challenges. Post-column derivatization separates donor and formaldehyde before derivatizing to a fluorescent product, avoiding equilibrium issues. The document describes a method using Pinnacle PCX for post-column derivatization of formaldehyde in samples using acetyl acetone to form a fluorescent derivative detected at 412/510 nm.

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    Formaldehyde: Application Note

    Uploaded by

    PaoloMM
    34 views3 pages
    Formaldehyde is used for disinfection but is also carcinogenic, so formaldehyde donors are used instead to maintain low free formaldehyde concentrations. Determining free formaldehyde is difficult due to its chemistry and detection challenges. Post-column derivatization separates donor and formaldehyde before derivatizing to a fluorescent product, avoiding equilibrium issues. The document describes a method using Pinnacle PCX for post-column derivatization of formaldehyde in samples using acetyl acetone to form a fluorescent derivative detected at 412/510 nm.

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    Application Formaldehyde

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    Formaldehyde is used for disinfection but is also carcinogenic, so formaldehyde donors are used instead to maintain low free formaldehyde concentrations. Determining free formaldehyde is difficult due to its chemistry and detection challenges. Post-column derivatization separates donor and formaldehyde before derivatizing to a fluorescent product, avoiding equilibrium issues. The document describes a method using Pinnacle PCX for post-column derivatization of formaldehyde in samples using acetyl acetone to form a fluorescent derivative detected at 412/510 nm.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    34 views3 pages

    Formaldehyde: Application Note

    Uploaded by

    PaoloMM
    Formaldehyde is used for disinfection but is also carcinogenic, so formaldehyde donors are used instead to maintain low free formaldehyde concentrations. Determining free formaldehyde is difficult due to its chemistry and detection challenges. Post-column derivatization separates donor and formaldehyde before derivatizing to a fluorescent product, avoiding equilibrium issues. The document describes a method using Pinnacle PCX for post-column derivatization of formaldehyde in samples using acetyl acetone to form a fluorescent derivative detected at 412/510 nm.

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    APPLICATION NOTE

    As of: April, 2010

    FORMALDEHYDE
    Formaldehyde is used for the disinfection of
    industrially manufactured products. However, it is det-
    rimental to health and its potential carcinogenicity led
    to the current utilization of formaldehyde donors for
    microbiological long-term stabilization, which are in
    hydrolytic equilibrium with formaldehyde. Thereby, the
    concentration of free formaldehyde is kept as low as
    possible.
    The quantitative determination of free formaldehyde
    seems quite problematic. Firstly, the detection charac-
    teristics of formaldehyde are very unfavorable. Con-
    sequently, derivatization is necessary in order to
    achieve the required sensitivity. Secondly, the hydro-
    lytic equilibrium between donor and free formaldehyde
    can change quite quickly, which makes the prepara-
    tion of samples more difficult and/or influences the
    correctness of analytical results.
    An elegant solution for this problem is, therefore, to
    perform the derivatization step after separation on an
    HPLC column, because the equilibrium cannot be in-
    fluenced when donor and free formaldehyde are
    chromatographically separated before derivatization. PICKERING offers the complete post-column
    derivatization system PINNACLE PCX for the detection of traces of formaldehyde. The user, how-
    ever, has to supply columns, eluents, reagents, etc..

    Description of the Method


    The derivatization of formaldehyde is performed in a one-step reaction using acetyl acetone
    (2,4-pentanedione). In the presence of an ammonium salt, it reacts to a yellow, strongly fluorescing
    3,5-diacetyl-1,4-dihydrolutidine derivative. The detection is performed with either a UV/VIS or fluo-
    rescence detector.

    LCTech GmbH I P.O. Box 1360 I 84403 Dorfen, Germany Seite 1 von 3
    Tel: +49-(0)8081-9368-0 I Fax: +49-(0)8081-9368-10 I www.Lctech.de I info@Lctech.de
    APPLICATION NOTE

    HPLC Conditions and Derivatization Parameters

    HPLC

    Operating Mode Isocratic

    Eluent Acetonitril:Water (85:15)

    Degassing Helium- or vacuum degassed

    HPLC Column RP C18

    Column Oven 40 °C

    Flow Rate 0.5 mL/min

    Post-column Derivatization

    Pinnacle PCX Single-pump

    Reactor volume 500 µL

    Reactor temperature 100 °C

    Reagents 0.81 M Ammonimn acetate /


    0.12 M Galicial acetic acid (pH=5)/
    0.05 M 2,4-Pentanedione with water

    Reagent Flow 0,4 mL/min

    Detection

    Detection Mode Fluorescence detection

    Excitation Wavelength 412 nm

    Emission Wavelength 510 nm

    Flowcell Analytic; pressure stable up to 7 bar

    LCTech GmbH I P.O. Box 1360 I 84403 Dorfen, Germany Seite 2 von 3
    Tel: +49-(0)8081-9368-0 I Fax: +49-(0)8081-9368-10 I www.Lctech.de I info@Lctech.de
    APPLICATION NOTE

    Chromatogram
    Chromatogram of a shampoo sample

    Sample spiked with 0,05% formaldehyde. Determination according to K 84.00-7(EG)

    Literature
    1) J. Meister, H. Engelhardt, LaborPraxis 1995, 7, 28 – 31.
    2) Amtliche Sammlung §64 LFGB, K 84.00-7(EG), September 1991.

    Order Information

    Order Number Description

    1153-1022 PINNACLE PCX – single-pump; 500 µL reactor

    LCTech GmbH I P.O. Box 1360 I 84403 Dorfen, Germany Seite 3 von 3
    Tel: +49-(0)8081-9368-0 I Fax: +49-(0)8081-9368-10 I www.Lctech.de I info@Lctech.de

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