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    Glycosides Lab Manual Pages 32-33: Qualitative Tests For Some Glycosides

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    إسراء رمضان
    The document discusses qualitative tests for several types of glycosides: anthraquinone glycosides, cardiac glycosides, flavonoid glycosides, cyanophore glycosides, and saponin glycosides. For each type of glycoside, examples of containing plants, uses, and a specialized chemical test are provided. Procedures and results are described for various tests including Borntrager's test, Keller-Killiani's test, Kedde's test, sodium hydroxide test, Guignard's paper test, and froth test.

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    Glycosides Lab Manual Pages 32-33: Qualitative Tests For Some Glycosides

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    142 views4 pages
    The document discusses qualitative tests for several types of glycosides: anthraquinone glycosides, cardiac glycosides, flavonoid glycosides, cyanophore glycosides, and saponin glycosides. For each type of glycoside, examples of containing plants, uses, and a specialized chemical test are provided. Procedures and results are described for various tests including Borntrager's test, Keller-Killiani's test, Kedde's test, sodium hydroxide test, Guignard's paper test, and froth test.

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    7) Qualitative tests for some Glycosides

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    7) Qualitative tests for some Glycosides

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    The document discusses qualitative tests for several types of glycosides: anthraquinone glycosides, cardiac glycosides, flavonoid glycosides, cyanophore glycosides, and saponin glycosides. For each type of glycoside, examples of containing plants, uses, and a specialized chemical test are provided. Procedures and results are described for various tests including Borntrager's test, Keller-Killiani's test, Kedde's test, sodium hydroxide test, Guignard's paper test, and froth test.

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    142 views4 pages

    Glycosides Lab Manual Pages 32-33: Qualitative Tests For Some Glycosides

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    إسراء رمضان
    The document discusses qualitative tests for several types of glycosides: anthraquinone glycosides, cardiac glycosides, flavonoid glycosides, cyanophore glycosides, and saponin glycosides. For each type of glycoside, examples of containing plants, uses, and a specialized chemical test are provided. Procedures and results are described for various tests including Borntrager's test, Keller-Killiani's test, Kedde's test, sodium hydroxide test, Guignard's paper test, and froth test.

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    12/25/2020

    Glycosides
    Qualitative Tests For Some Glycosides
    Lab Manual Pages 32-33
    • A N T H R A Q U I N O N E G LYC O S I D E S
    • C A R D I A C G LYC O S I D E S
    • F L AV O N O I D G LYC O S I D E S
    • C YA N O P H O R I C G LYC O S I D E S
    • S A P O N I N G LYC O S I D E S

    For every glycoside we should know:

    1. Examples of plants containing glycosides


    2. Uses of glycosides
    3. Chemical test for each glycoside.

    1
    12/25/2020

    Qualitative analysis of some glycosides


    Anthraquinone glycosides
    Examples Senna pods , Rhubarb , Cascara and Frangula
    Uses Purgatives “laxatives”
    Special Borntrager’s and modified Borntrager’s test
    test

    Procedures : ¼ spoon powder + 3 ml chloroform shake and filter


    using a wet piece of cotton add to the filtrate 1 ml 10% NH4OH
    Gentle shaking and set aside.

    Results : Rose pink to cherry red color in the aqueous layer

    N.B: This test is carried out for the combined glycosides but for the aglycone it will
    be carried out the same way but by omitting the step of boiling with Dil. H2SO4.

    Cardiac Glycosides
    Examples Digitalis , Nerium , Strophanthus and White Squill
    Uses Cardiotonic
    Special Keller-killiani’s Tests
    Tests Kedde’s test

    A) Keller-Killiani’s test : Done for glycosides having 2-deoxy sugars


    Reagents used:
    1. Glacial acetic acid
    2. FeCl3
    3. Conc. H2SO4
    Result : A brown to reddish brown layer is formed at the interface due to presence of
    aglycone and the upper glacial acetic acid layer becomes blue to bluish green due to the
    2-deoxy sugars.

    B) Kedde’s test : for the lactone ring


    Reagents used:
    1. Ethanol
    2. Dinitro benzoic acid in alcohol
    3. 5% NaOH

    Result : A violet color appears

    2
    12/25/2020

    Flavonoids glycosides
    Examples Sophora , Melia , Liqourice and
    Foenugreek
    Uses Antioxidant
    Special 10% NaOH test
    test
    Procedures :
    ¼ spoon of the powder + 5 ml of 1% HCl leave for
    10 mins
    Filter using a wet cotton add 1 ml of 10% NaOH
    drop wise

    Result : Yellow color appears due to the presence of


    flavonoids

    Cyanophore Glycosides

    Examples Linseed

    Uses Hypocholesterolemic

    Special Guignard’s paper test


    test

    Guignard’s paper test :

    1-Moisturize a filter paper in a beaker containing 1% picric acid solution


    followed by sodium carbonate Yellow color to the paper (guignard’s
    paper)

    2-In a stoppered test tube add 1 spoon of freshly prepared crushed linseed
    then add 5 ml of H2O at 37°C insert the guignard’s paper in the neck of
    the test tube and place it on a water bath for 30 minutes.

    Results :
    The color changes from yellow to orange or brick red colour due to the
    presence of HCN gas librated which reduces Na picrate into Na picramate

    3
    12/25/2020

    Saponin Glycosides
    Examples Liqourice and Quillaia , Sarsaparilla
    Uses Emulsifying agents , expectorants
    Properties - Formation of permanent froth with water
    - Typical type causes hemolysis of RBCs
    - Emulsifying agent and expectorant
    Physical CCC. Have bitter taste with a sternutatory effect
    Special test Froth test
    Froth test:
    ¼ spoon of the powder + 10 mls of water Heat till 1st boiling
    Filter

    Take 4 mls of the filtrate in a measuring cylinder add 4 mls of


    water and shake vigorously for 30 seconds then leave it for 1 min

    Result: Persistent froth ≥ 0.5mls

    N.B. Hemolysis test differentiates between Typical and Non-Typical


    Saponins.

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