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    Sodium Reagent Kit (Mono Test) : Colorimetric Method

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    Fazal Raza
    This document summarizes a sodium reagent kit for the quantitative determination of sodium in serum. The kit uses a colorimetric method where sodium reacts with a selective chromogen to produce a chromophore whose absorbance is directly proportional to the sodium concentration. The kit contains sodium reagent 1, sodium standard 150 mEq/L, and instructions for serum collection, reagent preparation and storage, assay procedure, calculation, quality control, and normal values.

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    Sodium Reagent Kit (Mono Test) : Colorimetric Method

    Uploaded by

    Fazal Raza
    533 views1 page
    This document summarizes a sodium reagent kit for the quantitative determination of sodium in serum. The kit uses a colorimetric method where sodium reacts with a selective chromogen to produce a chromophore whose absorbance is directly proportional to the sodium concentration. The kit contains sodium reagent 1, sodium standard 150 mEq/L, and instructions for serum collection, reagent preparation and storage, assay procedure, calculation, quality control, and normal values.

    Original Description:

    Sodium hydroxide

    Original Title

    Sodium Mono

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    This document summarizes a sodium reagent kit for the quantitative determination of sodium in serum. The kit uses a colorimetric method where sodium reacts with a selective chromogen to produce a chromophore whose absorbance is directly proportional to the sodium concentration. The kit contains sodium reagent 1, sodium standard 150 mEq/L, and instructions for serum collection, reagent preparation and storage, assay procedure, calculation, quality control, and normal values.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    533 views1 page

    Sodium Reagent Kit (Mono Test) : Colorimetric Method

    Uploaded by

    Fazal Raza
    This document summarizes a sodium reagent kit for the quantitative determination of sodium in serum. The kit uses a colorimetric method where sodium reacts with a selective chromogen to produce a chromophore whose absorbance is directly proportional to the sodium concentration. The kit contains sodium reagent 1, sodium standard 150 mEq/L, and instructions for serum collection, reagent preparation and storage, assay procedure, calculation, quality control, and normal values.

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    Sodium Reagent Kit (Mono Test)

    Colorimetric Method
    INTENDED USE :
    This reagent kit is intended for the “In Vitro” quantitative
    determination of Sodium in Serum.

    PRINCIPLE: LINEARITY :
    The method is based on reaction of sodium with a selective This procedure is linear upto 180 mEq/L. If values exceed
    Chromogen producing a chromophore whose absorbance is this limit dilute the sample with distilled water and multiply
    directly proportional to sodium concentration in the sample results with proper dilution factor.

    CONTENTS : NOTE :
    Reagent 1 : Sodium Reagent As Sodium is a very widely distributed ion, care should be
    Reagent 2 : Sodium Standard 150 mEq/L taken to avoid any contamination. All glasswares being
    used for the test should first be rinsed with 1% or 0.1 N
    SAMPLE COLLECTION AND PRESERVATION : HNO3 and then with good quality deionised water before
    Serum or heparinized plasma. use.
    Sodium is stable for 2 weeks at 2 - 8°C .
    BIBILOGRAPHY :
    REAGENT PREPARATION AND STORAGE : 1. Tietz, N.W., Fundamentals ofclinical Chemistry, W.b.
    All reagents are ready to use. Store reagents at Room Temperature Saunders Co. Phila, P.A. p. 874.
    (25 - 30°C). 2. Henry R.F., et, al, Clinical Chemistry Principles and Technics.
    2nd Ed, Harper and Row, Harper and Row, Hargersein, M.D.
    REAGENT STABILITY : (1974)
    The reagents are stable until the expiration date mentioned on the 3. Maruna RFL., Clin Chem. Acta. 2:581, (1958)
    kit. 4. Trinder, P:Analyst, 76:596, (1951)

    GENERAL SYSTEM PARAMETERS :


    Reaction type : End point Code No. Pack Size Reagent 1 Reagent 2
    Wavelength : 630 nm S20 25T 25 x 1.0 ml 3.0 ml
    Cuvette : 1 cm
    Reaction Temperature : RT
    Zero setting : Reagent blank
    IVD
    UNIONPROJET S.R.L.S.
    Reagent volume : 1.0 ml EC REP Via Aliprandi 41-20851 www.jas-anz.com.au/register

    Lissone (MB) ISO 9001:2015 ISO 13485:2003


    Sample volume : 10 µl
    Incubation time : 5 mins
    Blank absorbance limit : 1.2
    Standard concentration : 150 mEq/L

    ASSAY PROCEDURE :
    Pipette into clean dry testtubes labeled as Blank (B), Standard (S),
    and Test (T) :

    Addition Sequence (B) (S) (T)


    Sodium reagent 1.0 ml 1.0 ml 1.0 ml
    Standard - 10 µl -
    Sample - - 10 µl

    Mix well and incubate at RT for 5 mins. Measure the absorbance of


    Standard (Abs.S) and Test (Abs. T) against reagent blank at 630
    nm.

    CALCULATION :
    Concentartion of Sodium (mEq/L) : Abs. T
    X 150
    Abs. S

    QUALITY CONTROL :
    It is recommended that controls be included in each set of assays.

    NORMAL VALUES :
    Serum/Plasma : 135 - 155 mEq/L.
    It is recommended that each laboratory establish its own normal
    range represnting its patient population.

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