2017LR75 - SUMMARY REPORT Final 03052020

MicroVal Study 2017LR75: Confirmation Method
Validation study of the MALDI Biotyper® Complete Solution as an Alternative

Method for the Confirmation of Listeria spp.
MCS ILS, Version 2

March 5, 2020
Contact Details:
Q Laboratories
1930 Radcliff Dr.
Cincinnati, OH 45204
Tel: +1-513-471-1300
www.qlaboratories.com
Report issued and authorized by:

Q Laboratories
Benjamin Bastin, Microbiology R&D Supervisor
The report was prepared in accordance with ISO 16140-6:2019 and the most recent
version of the MicroVal Technical Committee for interpretation on ISO 16140-6,
version 2.0.
Company: Bruker Daltonics
Expert Laboratory: ADRIA Development (FR) and Q Laboroatories (OH,USA)
Method/Kit name: MALDI Biotyper for Listeria spp. confirmation
Validation standard: ISO 16140-6: 2019 Microbiology of food chain – Method
Validation–Part 6: Protocol for the validation of alternative (proprietary) methods for
microbiological confirmation and typing procedures.
Reference methods:
ISO 11290-1 (May 2017): Microbiology of the food chain - Horizontal method for the
detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1:
detection method
Scope of validation: confirmation of Listeria spp. and Listeria monocytogenes

presumptive colonies isolated on O&A, Palcam, Oxford, MOX and RAPID’L.mono,
and a non-selective agar, Tryptone Soja Agar with yeast Extract was used for tha
purpose (TSYEA).
Certification organization: Lloyd's Register
List of abbreviations
- No typical colonies but presence of background microflora
AL Acceptability Limit
Alt Alternative method
BG- Gram negative rod
CBA Columbia blood agar
d Doubtful result
DT Direct transfer procedure
eDT Extended direct transfer procedure
EXT Extraction procedure
EL Expert Laboratory
g Gram
h Hour
MALDI- Matrix Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF)
TOF Maldi Biotyper
MBT Mass Spectrometry
MS
mCCDA Modified charcoal-cefoperazone-deoxycholate agar
MCS Method Comparison Study
min minute
ml millilitre
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MR (MicroVal) Method Reviewer
MSP Main Spectrum or Main Spectra
MVTC MicroVal Technical Committee
NA Negative agreement
ND Negative deviation
NOIP No organism identification possible
PA Positive agreement
PD Positive deviation
pos (+) positive/growth/target detected
RT Relative Trueness
TSA Tryptic Soy Agar
w Weak reaction
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Contents
Introduction 6
1 Method protocols 6
1.1 Reference method 6
1.2 Alternative method 6
1.2.1 Instrumentation Workflow and Mechanism 7
1.2.2 Calculating a log (score) value and interpretation 8
1.2.3 Instrumentation Features 10
1.2.4 Software Features 11
1.2.5 Targets and biotargets 11
1.2.6 Associated devices 11
1.2.7 Consumables 12
1.3 Study design 12
2 Method comparison study 12
2.1 Selection of Test Strains 12
2.2 Samples to be tested 13
2.3 Inclusivity and exclusivity 13
2.3.1 Data discrepancy 14
2.3.2 Listeria spp. testing at the genus level 14
2.3.3. Summary of the inclusivity and exclusivity testing 15
2.3.3 Expression of results 15
2.3.4 Evaluation 21
5 Interlaboratory Study 29
Protocol and Materials 29
Transport 30
Analysis 30
Statistics and Results 30
6 Conclusions of Interlaboratory Study 40
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Annex A - Flow diagrams of the reference methods 42
Annex B - Flow diagram of the alternative method 44
Annex B - Kit inserts 46
Annex C – Certificate of equivalence 55
Annex D – Listeria spp. and Listeria monocytogenes testing 56
Annex E - Raw data inclusivity study 57
Annex F - Raw data exclusivity study 58
Annex G - Results inclusivity study: 59
Annex H - Results exclusivity study- Listeria spp. 79
Annex I - Results inclusivity study - Listeria monocytogenes 95
Annex K - Results exclusivity study - Listeria monocytogenes 107
Annex L - List of collaborators 127
Annex M - List of strains: List of strains tested in the inter-laboratory study 128
Annex N - Listeria spp. and Listeria monocytogenes testing at the genus level 129
Annex O - Raw data inter-laboratory study 130
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Introduction
The purpose of this MicroVal study was to validate the use of the Bruker MALDI
Biotyper® (MBT) using a presumptive Listeria spp. (defined by ISO 11290-1:2017) as
an alternative method to ISO 16140-6:2019. Only the rates of confirmed and non-
confirmed results were considered in the final interpretation. Additional details about
the strains tested with the MALDI Biotyper are provided in the report for information
purposes only.
The MBT utilizes Matrix Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-

TOF) Mass Spectrometry (MS) for the rapid and accurate confirmation and
identification of micro-organisms isolated from select media types. The MBT method
is designed to rapidly confirm a presumptive result and identify the isolates from a
variety of agar plates.
The alternative method was evaluated to confirm Listeria spp and Listeria
monocytogenes according to the technical rules described in the ISO 16140-Part 6 -
Microbiology of the food chain - Method validation - Part 6: protocol for the validation
of alternative (proprietary) methods for microbiological confirmation and typing
procedures. The tested microorganisms were isolated on Ottaviani and Agosti Agar
(O&A), Palcam, Oxford, Modified Oxford (MOX), RAPID’L.mono and a non-selective
agar. Two MBT systems were tested, the MBT with the microflex LT/SH instrument,
and the MBT smart with the microflex LT/SH smart instrument. Half of the method
comparison study was run at ADRIA (FR); the other part was run at Q-Laboratories
(OH, USA). The inter-laboratory study was organized in Europe, by involving 16
collaborators from different laboratories.
Reference method: ISO 11290:2017
The scope of the validation study is the confirmation of Listeria spp. and Listeria
monocytogenes presumptive colonies isolated on O&A, Palcam, Oxford, MOX and
RAPID’L.mono, and a non-selective agar, Tryptone Soja Agar with yeast Extract was
used for tha purpose (TSYEA).
1 Method protocols
1.1 Reference method
See the flow diagram in Annex A.

The reference methods correspond to the ISO 11290-1 (May 2017) - Microbiology of
the food chain - Horizontal method for the detection and enumeration of Listeria
monocytogenes and other Listeria spp. - Part 1: detection method; and the ISO
11290-2 (May 2017) - Microbiology of the food chain - Horizontal method for the
detection and enumeration of Listeria monocytogenes and other Listeria spp. - Part
2: enumeration method.
1.2 Alternative method
See the flow diagram of the alternative method in Annex B.
See the link below corresponding to clinical applications.
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https://www.youtube.com/watch?v=aD7Ln71wGFM
1.2.1 Instrumentation Workflow and Mechanism
An individual colony from a culture plate of the tested strain was transferred to a
selected position on a MALDI target plate. The MALDI target plate was air-dried and
the matrix was added. The matrix solution was used to extract mostly ribosomal
proteins at high concentrations from the microorganisms. When the matrix
crystallized and was completely dry, the MALDI target plate was analyzed using the
MBT System microflex LT/SH or the MBT System microflex LT/SH smart with the
corresponding MBT software (4.0 and Compass versions).
Strains were analyzed using MALDI-TOF MS. A laser in the MALDI-TOF MS

irradiates the dried matrix spot. The rapid evaporation of the matrix and proteins
caused by the laser resulted in the release of intact, positively charged proteins and
peptides (a "soft" ionization technique). These ions are electrostatically accelerated
over a short distance and arrive in the flight tube at a mass-dependent speed. As
different proteins/peptides have different masses, ions arrive at the detector at
different times (Time Of Flight). The mass spectrometer measures the time (in the
microsecond range) between pulsed acceleration and the corresponding detector
signal. The speed is converted into a molecular mass. The highly abundant
microbial ribosomal proteins result in a mass spectrum with a characteristic mass and
intensity distribution pattern. For many microorganisms, this pattern is species-
specific and can be used as a “molecular fingerprint” to identify the sample.
The MBT Explorer Module allows sophisticated statistical analysis as well as the
extension of existing libraries or the creation of new libraries; statistical analysis is
supported by PCA (Principal Component Analysis), Clustering (dendrogram creation
and examination) and correlation analysis based on a composite correlation index
(CCI) matrix approach.
The MALDI Biotyper (MBT) software and library:
• The MBT System microflex LT/SH (MBT) or MBT System microflex LT/SH
smart (MBT-smart) instruments are benchtop MALDI-Time-Of-Flight Mass
Spectrometers for accurate mass determination of biomolecules.
• The MBT Compass or MBT 4.0 Software are dedicated software packages
for microorganism identification based on MALDI-TOF MS profile spectra. The
MBT Compass software controls mass spectrometry data acquisition and
matches acquired spectra against the MBT Compass library for final
identification results. The software generates a report using score values and
scoring symbols.
• The MBT Compass Library includes defined bacteria and yeast strains from
industrial, veterinary, clinical isolates provided by collaborating partners, round
robin strains and strains from accredited ISO 9001:2008 certified strain
collections.
• This validation was run with the MBT Compass Library version 6903 and was,
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therefore, included as part of the validation scope.
The eDT and EXT sample preparations were used when recommended, based on
the calculating log (score) value. The sample stability onto prepared targets was
guaranteed up to 24 hours at 20 - 25°C.
1.2.2 Calculating a log (score) value and interpretation
Data acquisition was controlled and analyzed using MBT Compass software. The
spectrum of the unknown sample was first translated into a peak list. Using a
biostatistical algorithm, this peak list was compared to reference peak lists of
organisms in the reference library database and a log (score) between 0.00 and 3.00
was generated. The higher the log (score), the higher the degree of similarity
between the pattern for the unknown peak list and the peak list for the database entry
in the reference library.
The MBT Subtyping module supports the identification of the mostly encountered
Listeria species in food, feed and related environmental samples: L. innocua, L.
ivanovii, L. monocytogenes, L. seeligeri, L. welshimeri; this module is not required for
L. grayi identification. The Subtyping module starts automatically and assesses the
quality of the profiles, before checking the specific biomarkers for species
identification. The module differentiates L. monocytogenes from the closely related
L. innocua, L. ivanovii, L. monocytogenes, L. seeligeri, L. welshimeri species. This
MBT Compass Subtyping module is therefore required to confirm L. monocytogenes.
The other genera and related species were identified using the Consistency Category
of the MBT Compass Manual. Scores >2.00 were presented in green and were
determined to be acceptable with high confidence identification at the species level
(both Listeria species and non-Listeria organisms), e.g. a score >2.00 is therefore
required to identify L. grayi. Results presented in yellow have values between 1.70
and 1.99 and were considered low confidence identification. Results presented in
red were considered not acceptable for identification.
The consistency category of the identification was based on the confidence level of
the best and second-best matches as described below. If the log (score) is < 1.70
after initial analysis, the DT sample preparation was repeated, and if needed the
alternative sample preparation procedures, eDT and EXT, was run (See Annex C).
If the identification is still not possible, it was advised to streak on a non-selective
agar. Figure 1 shows the workflow.
The MBT report dedicated to food microbiology applications displays directly the
confirmation results of Listeria spp and L. monocytogenes, and the identification of
other isolates.
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Figure 1
Isolated colonies on appropriate medium
Perform Direct Transfer (DT).

Analyze on MALDI Biotyper (MBT)
Release the results for

Genus or species L. monocytogenes
YES NO YES YES
Release the Listeria species differentiation Conclusive Listeria species confirmation or Listeria spp
identification based on the
identification result with scores  2,00* differentiation* confirmation including the
consistency category table
identified species when
appropriate
NO NO
Repeat by running DT, Repeat by running DT,

Extended Direct Tranfer Extended Direct Tranfer
(eDT) until Extraction (EXT) (eDT) until Extraction (EXT)
Release the results for

Genus or species L. monocytogenes
YES YES
Release the identification based on the Conclusive Listeria species confirmation or Listeria spp
identification result consistency category table differentiation* confirmation including the
identified species when
NO appropriate
NO
Streak onto non selective Streak onto non selective

agar and repeat the agar and repeat the
workflow workflow
*Listeria genus is confirmed with scores  1,70, while L. monocytogenes is differentiated from the L. innocua, L. ivanovii,
L. seeligeri and L. welshimeri with the MBT Compass Subtyping Module. However, L. marthii cannot be differentiated from
L. monocytogenes. Note that usually L. marthii isolates doesn’t show halo on Ottaviani & Agosti Agar and on RAPID’L. mono
(Bio-Rad). L. grayi is identified without the need of MBT Compass Subtyping Module. As only a few strains of the newly
Listeria species are available, the MBT Library contains only a few reference spectra. These species can be only identified at
the genus level.
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The MBT report for Food Microbiology, activated in the MBT Compass software,
provided the confirmation and identification results according to this flowchart.
The Quick Guide for Food Testing was provided in addition to the MBT Compass
user manual, in order to describe the scope of the ISO 16140-6 validation, the
workflow, the controls, and the interpretation. Note that L. marthii cannot be
differentiated from L. monocytogenes. Typically, L. marthii isolates do not show a
halo on Ottaviani & Agosti Agar and on RAPID’L.mono. Since only a few strains of
the recently described Listeria species are available, the MBT Library contains a
limited number of reference spectra. The identification is only guaranteed at the
genus level. The MBT Library 6903 contains only one reference profile of the two
newly described Listeria species: L. fleischmannii and L. weihenstephanensis.
1.2.3 Instrumentation Features
As previously stated, two MBT Complete Solutions are currently available depending
on the instrument, MBT System microflex LT/SH (MBT) or MBT System microflex
LT/SH smart (MBT-smart). The two instruments were tested during the validation:
the MBT microflex LT/SH by the expert laboratory. The blind-coded biotargets were
sent for a second reading by BRUKER (Europe) and Atlanta Hospital (US) using the
MBT-smart. Bruker internal data were available to support the sample stability after
the matrix addition up to 24 hours at 20-25°C, but in some cases, the biotargets
arrived one day later. The differences result from the laser technology and the
vacuum system are presented below (See Table 1).
Table 1. A Comparison of the laser technology and the vacuum system
MALDI Biotyper System MALDI Biotyper Smart System

microflex LT/SH microflex LT/SH
Vacuum system High-performance vacuum system
Gridless microSCOUT™ MALDI ion source Gridless microSCOUT™ MALDI ion source with
with extended pulsed ion extraction PAN™ extended pulsed ion extraction PAN™ mass range
mass range focusing focusing
Integrated source cleaning without the need of Integrated source cleaning without the need of
venting the system venting the system
60 Hz nitrogen laser 200 Hz BRUKER smartbeam™ solid-state lifetime

laser
TOF analyzer for linear-only measurements TOF analyzer for linear-only measurements with
with positive acceleration potential positive acceleration potential
Data System with digitizer, WIN7 Operating- Data System with digitizer, WIN7 Operating-
System, >=24" LCD-Display, Printer and System, >=24" LCD-Display, Printer and
Compass™ for Flex software package for MS Compass™ for Flex software package for MS
control, data acquisition and processing control, data acquisition and processing
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1.2.4 Software Features
Two versions of the software are currently available: MBT Compass and MBT 4.0.
They use the same algorithm and thus provide the same identification result, as
presented in the certificate of equivalence (See Annex D).
The only differences in the two software versions are the following:
• The MBT Compass enables the use of the Subtyping Module for automated
detection of strain specific characteristics, while the MBT 4.0 cannot;
• The MBT 4.0 enables electronical signature and audit trail with the MBT Security
Pack, while the MBT Compass still must be implemented for that option.
1.2.5 Targets and biotargets
The samples can be plotted on several targets:

• Reusable 48 and 96 polished steel target;
• Disposable Biotarget 96, which can be used until all the sample positions are
filled.
To clearly show the equivalence, 7 collaborators involved in the ILS used disposable
biotargets and 8 collaborators ran the tests on reusable targets. The MCS was run
with disposable biotargets to simplify the workflow and, therefore, no washing step
was required. These targets were simply used to show equivalence with materials to
plot the samples and data acquired to comply with the ISO 13485 and ISO 9001
certification.
1.2.6 Associated devices
The MBT was associated to the following devices in order to facilitate the workflow:
• MBT Pilot workstation supported correct sample positioning by guided MALDI

target preparation through micro-projection technology; MBT Galaxy
automated target preparation system frees laboratory personal from routine
work, using exactly the same sample procedure as the manual one; indeed,
no additional testing was required, and data acquired to comply with the ISO
13485 and ISO 9001 certification were available to show the equivalence.
Note that the MBT Galaxy is not a colony picker, the MBT Galaxy runs only
automatic pipetting of the HCCA (α-cyano-4-hydroxycinnamic acid) matrix of
formic acid. Since automation steps were identical to those used in the
manual process, it was only necessary to use the manual method within the
validation.
• Barcode Scanner was used for easy reading of barcodes of routine samples.
For additional information, please see: https://www.bruker.com/products/mass-

spectrometry-and-separations/ivd-ce-certified-maldi-biotyper/consumables-
accessories-for-mbt-workflows.html
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1.2.7 Consumables
The following consumables were required for the manual procedure:
• HCCA Matrix for the sample preparation;

• Bacterial Test Standard (BTS), i.e. mass calibration standard showing a typical
Escherichia coli DH5 alpha peptide and protein profile plus additional proteins
in MALDI-TOF MS. It is mandatory to get at least one valid BTS control per
run.
Additionally, formic acid was used for the extended direct transfer, as well as ethanol
and acetonitrile for the extraction protocol.
1.3 Study design
Microorganisms identified in this MCS using the MBT were isolated by streaking a
microbial culture onto an agar plate.
The MBT workflow gathers 3 sample preparation procedures, which are presented in
Annex B:
1. Direct transfer sample preparation procedure (DT), which is the usual procedure
for most of the bacterial isolates and is used as the general procedure;
2. Extended direct transfer sample preparation procedure (eDT);
3. Extraction sample preparation procedure (EXT).
2 Method comparison study
2.1 Selection of Test Strains
A range of strains were used. The selection of the tested strains was done according
to the Annex A of the ISO 16140 – part 6. The strains were selected by considering
the measurement principle of the alternative method. Different measurement
principles may require the use of different test panels of strains. In addition, the
occurrence of suspect and interfering colonies on the media used for the reference
method was of importance for selecting strains.
Each strain was characterized biochemically, serologically and/or genetically for it to

be correctly identified. Strains were isolated mostly from foods, feed, the food-
processing environment, or from primary production in consideration of the scope of
the validation. Clinical, environmental and culture collection strains could also be
used. The original source of the isolates should be traceable and held in a local (e.g.
expert laboratory), national or international culture collection to enable them to be
used in future testing if required.
The selected strains from the inclusivity and exclusivity lists were coming from
collections of the expert laboratories or from culture type collections. They were all
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identified at the species level using appropriate methods, conventional or molecular
identification.
2.2 Samples to be tested
Highly characterized pure strains were used for confirmation procedures following the
requirements as specified in ISO 7218 and ISO 11290-1 and 2.
The validation study was carried out using strains cultured from stock on a non-
selective medium or stored on microbeads at -80°C.
Required previously generated data were available for the reference confirmation
procedure, therefore, it was not necessary to repeat the reference confirmation
procedure with the alternative confirmation method for the MCS.
The confirmatory tests described in the ISO 11290-1 and ISO 11290-2 methods were
applied on the strains for the inter-laboratory study. The xylose and rhamnose were
tested using biochemical galleries.
2.3 Inclusivity and exclusivity
The study design was based on the taxonomy level addressed by the alternative
method and covered by the scope of the certification. All the strains were blind
coded prior to analysis.
Inclusivity
Pure cultures of all strains were required to be tested with the reference confirmation
procedure and with the alternative confirmation method. 155 different target strains
were teste, including 9 newly described species. In the case of Listeria
monocytogenes, 100 different strains were tested.
Exclusivity
Pure cultures of all strains were required to be tested with the reference confirmation
procedure and with the alternative confirmation method. 107 non-target strains (non-
Listeria spp) were tested. In the case of Listeria monocytogenes, 50 different non-
taget (non-L. monocytogenes) strains were tested.
124 Listeria spp. strains (non-Listeria monocytogenes strains) were tested; in order
to fit with the ISO 16140-6 requirements, only 50 strains from the most widely
encountered species were kept for interpretation done for L. monocytogenes
confirmation purpose:
- 10 Listeria innocua,
- 10 Listeria grayii,
- 10 Listeria ivanovii,
- 10 Listeria seeligeri,
- 10 Listeria welshimeri.
The data were completed with 50 non-Listeria strains from the exclusivity panel to
reach the required number of 100 non-target strains.
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Some of the strains from the exclusivity lists were not able to grow or to show
characteristic colonies on the tested selective agars. The strains used in exclusivity
testing that exhibited any growth were tested with the MBT procedure to ensure there
was no cross-reactivity with the chosen selective agar. The characteristics of the
colonies are shown in the raw data.
2.3.1 Data discrepancy
In case of discrepancy (ies) between the alternative and reference methods, the
strains were further identified by sequencing the 16S rDNA target. This was the case
for some exclusivity strains, but there was no impact on the outcomes of the Listeria
confirmations.
2.3.2 Listeria spp. testing at the genus level
155 Listeria spp. strains and 107 relevant non-Listeria spp. strains were tested.
Each strain was first recovered on a non-selective agar (TSYEA) incubated at 37.0 ±
1 °C for 24 h ± 3 h, as well as on five selective agars:
- O&A incubated at 37.0 ± 1 °C for 24 h ± 3 h;

- Palcam incubated at 37.0 ± 1 °C for 24 h ± 3 h;
- Oxford incubated at 37.0 ± 1 °C for 24 h ± 3 h;
- MOX incubated at 37.0 ± 1 °C for 24 h ± 3 h;
- RAPID’L.mono incubated at 37.0 ± 1 °C for 24 h ± 3 h.
Non-target organisms were incubated under optimal growth conditions for the
organism. All plates with growth were analyzed by the Bruker Maldi Biotyper System
on disposable targets, following the Direct Transfer sample preparation. An isolated
colony from each plate was smeared as a thin film directly onto a single sample
position on the disposable target. A 1 µL aliquot of BTS was spotted onto a
designated position in the disposable target and allowed to dry. After the spotting of
the target plate, the HCCA matrix was added within 30 minutes. After the addition of
the matrix to the smear, the target plate was analyzed within 24 hours. The protocol
is described in Annex D .
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2.3.3. Summary of the inclusivity and exclusivity testing
Table 2 outlines the tests which were done for Listeria spp. Table 3 displays the
tests which were done for Listeria monocytogenes. Only strains showing colonies
were tested.
Table 2. Tests carried out during the MCS

Inclusivity
Target Exclusivity Total
Media tested Tests number
TSYEA 155 107
O&A 155 9
Palcam 150 12
Listeria spp /
Oxford 155 23
MOX 151 8
RAPID'L.mono 140 11
TOTAL 906 170 1076
Table 3 – Tests done during the MCS (Listeria monocytogenes)

Inclusivity
Target Exclusivity Total
Media tested Tests number
TSYEA 100 100
O&A 100 55
Listeria Palcam 100 50
/
monocytogenes Oxford 100 65
MOX 100 53
RAPID'L.mono 100 50
TOTAL 600 373 973
2.3.3 Expression of results
The results were tabulated in Annex E for Listeria spp. inclusivity and Annex F for
Listeria spp. exclusivity study. The results were tabulated in Annex G for Listeria
monocytogenes inclusivity and Annex H for Listeria monocytogenes exclusivity
study.
The following tables give the number of strains showing non-typical growth on the
tested plates for Listeria spp. (See Table 4) and Listeria monocytogenes (See Table
5) confirmation as well as the number of strains for which a subculture on TSYEA
was required to get an identification using the MBT-smart. The strains number 92,
94, 95, 96, 133 and 134 correspond to newly described Listeria species.
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Table 4. Description of the observed culture data for Listeria spp.
Number of strains Number of sub-culture on non selective agar
Study Media giving non required to get a confirmation result
characteristic colonies MBT MBT smart
3 (N 94-95- 5 (92-94-95-133-
TSYEA 0
133)=NOIP 134)=NOIP
O&A 4/155 4 (N 95-96-133-134) 5 (N 92-94-95-133-134)
Palcam 0/155 3 (N 95-96-133) 5 (N 94-95-96-133-134)
Inclusivity 5 (N 92-94-95-96- 6 (N 92-94-95-96-133-
Oxford 0/155
133- 134)
MOX 0/155 3 (N 94-95-133) 4 (N 94-95-133-134)
6 (N 92-94-95-96-133-
RAPID'L.mono 11/155
4 (N 94-95-133-134) 134)
4 (N 58-75-76-107)= 3 (N 5-6-63)=NOIP
TSYEA 0 No peak 4 (N 58-75-76-107)=
1 (N 63)=NOIP No peak
O&A 6/107 0 0
Exclusivity
Palcam 10/107 0 0
Oxford 16/107 2 (N 39 - 42) 1 (N 42)
MOX 3/107 0 1 (N 39)
RAPID'L.mono 12/107 0 1 (N 39)
NOIP: No organism identification possible
Table 5. Description of the observed culture data for Listeria monocytogenes

Number of strains Number of sub-culture on non selective agar
giving non required to get an identification result
Study Media
characteristic
MBT MBT smart
colonies
TSYEA 0 0 0
O&A 0/100 0 0
Palcam 0/100 0 0
Inclusivity
Oxford 0/100 0 0
MOX 0/100 0 0
RAPID'L.mono 0/100 0 0
TSYEA 0 0 0
O&A 4/100 0 0
Palcam 5/100 0 0
Exclusivity
Oxford 11/100 2 (N 43-45) 1 (N 45)
MOX 3/100 0 1 (N 43)
RAPID'L.mono 12/100 0 1 (N 43)
For Listeria spp. inclusivity panel, all the strains were able to grow on the non
selective agar plate (TSYEA), but some strains didn’t grow on some selective agar
plates: for Palcam, 3 L.grayi and 2 L.seeligeri; on MOX: 4 L.grayi; and on
RAPID’L.mono: 11 L.grayi, 1 L.cornellensis, 2 L.seeligeri, 1 L.weihenstephenensis.
For the Listeria monocytogenes inclusivity panel specifically, all the strains were able
to grow on the non selective agar plate.
For Listeria spp. exclusivity panel, 98 strains didn’t grow on Palcam plates, 84 on
Oxford, 96 on MOX and 94 on RAPID’L.mono.
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For the Listeria monocytogenes exclusivity panel specifically, 45 strains didn’t grow
on O&A plates, 50 on Palcam, 35 on Oxford, 47 on MOX and 50 on RAPID’L.mono.
For Listeria monocytogenes inclusivity and exclusivity panels, all the strains gave a
result.
For inclusivity and exclusivity panels, the eDT (extended direct transfer) and EXT
(extraction) procedures were used in some cases. This is summarised in Table 6 for
Listeria spp and Table 7 for Listeria monocytogenes.
Table 6. Use of the eDT and EXT Procedures in the MBT workflow for Listeria
spp.
MBT MBT smart
Target Media No growth
DT eDT EXT DT eDT EXT
TSYEA 0 149 3 3 150 2 3
O&A 0 150 0 5 150 0 5
Palcam 5 146 1 3 137 5 8
Oxford 0 146 3 6 138 3 14
Inclusivity MOX 4 147 1 3 141 3 7
RAPID'L.mono 15 130 4 6 113 10 17
868 12 26 829 23 54
Total 24
906 906
Percentage / 95.8% 1.3% 2.9% 91.5% 2.5% 6.0%
TSYEA 0 98 7 2 97 5 5
O&A 98 9 0 0 9 0 0
Palcam 95 12 0 0 12 0 0
Oxford 84 21 0 2 21 1 1
Exclusivity MOX 99 8 0 0 7 0 1
RAPID'L.mono 96 11 0 0 10 0 1
159 7 4 156 6 8
Total 472
170 170
Percentage / 93.5% 4.1% 2.4% 91.8% 3.5% 4.7%
Inclusivity study
For the MBT, the eDT was run for 1.3% of the identification results and the EXT for
2.9%. For the MBT-smart, the eDT was run for 2.5% identification results and the
EXT for 6.0%.
Exclusivity study
EXT for 4.7%.
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Table 7 - Use of the eDT and EXT Procedures in the MBT workflow
for Listeria monotytogenes
MBT MBT smart
Target Media No growth
DT eDT EXT DT eDT EXT
TSYEA 0 99 1 0 100 0 0
O&A 0 97 2 1 98 2 0
Palcam 0 99 1 0 96 4 0
Oxford 0 97 3 0 99 1 0
Inclusivity MOX 0 100 0 0 98 2 0
RAPID'L.mono 0 97 2 1 90 5 5
589 9 2 581 14 5
Total 0
600 600
Percentage / 98.2% 1.5% 0.3% 96.8% 2.3% 0.8%
TSYEA 0 98 1 1 99 0 1
O&A 45 55 0 0 55 0 0
Palcam 50 50 0 0 50 0 0
Oxford 35 63 0 2 59 3 3
Exclusivity MOX 47 53 0 0 50 0 3
RAPID'L.mono 50 48 1 1 41 5 4
367 2 4 354 8 11
Total 227
373 373
Percentage / 98.4% 0.5% 1.1% 94.9% 2.1% 2.9%
Inclusivity study
EXT for 0.8%.
Exclusivity study
EXT for 2.9%.
For the MBT-smart, the biotargets were analysed 24 hours (minimum) after the
sample preparations for ADRIA. The observed slight differences in the sample
preparation conducting to an identification result are probably due to the 24 h delay
before running the reading with the MBT smart. Since both the MBT and MBT Smart
instruments were available at Q Laboratories at that time, no differences were
observed.
The interpretation of the results is summarized in Table 8 for Listeria spp and Table
9 for Listeria monocytogenes; the first step of the confirmation procedure is the
isolation on the tested culture media.
The data and interpretation confirm the reliability and robustness of the MALDI
Biotyper as a confirmation method for Listeria spp and non-Listeria spp isolates, L.
monocytogenes and non-L. monocytogenes isolates from isolated colonies.
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Table 8 - Summary of the results in the method comparison study Listeria spp
MALDI System Media tested Study N IA ID EA* ED
Inclusivity 155c 152 0 Not applicable Not applicable

TSYEA
Exclusivity 107e Not applicable Not applicable 102 0
Inclusivity 155d 151 0 Not applicable Not applicable
O&A
Exclusivity 107 Not applicable Not applicable 9 0
Palcam
LT/SH-MALDI-MS System
Inclusivity 155e 150 0 Not applicable Not applicable
Oxford
Exclusivity 107b Not applicable Not applicable 21 0
MOX
RAPID'L.mono
TSYEA
Exclusivity 107g Not applicable Not applicable 100 0
O&A
Palcam
LT/SH MALDI Smart System
Inclusivity 155f 149 0 Not applicable Not applicable
Oxford
Exclusivity 107a Not applicable Not applicable 22 0
MOX
Inclusivity 155f 134 0 Not applicable Not applicable
RAPID'L.mono
a
No organism identification possible (NOIP) for 1 out of 107
b
c
d
e
f
g
*Studies with EA< N are due to no growth and/or the indicated NOIP
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Table 9: Summary of the results in the method comparison study – Listeria monocytogenes
MALDI System Media tested Study N IA ID EA* ED
Inclusivity 100 100 0 Not applicable Not applicable

TSYEA
O&A
Palcam
Oxford
Exclusivity 100b Not applicable Not applicable 63 0
MOX
RAPID'L.mono
TSYEA
O&A
Palcam
Oxford
Exclusivity 100a Not applicable Not applicable 63 0**
MOX
RAPID'L.mono
a
b
*Studies with EA< N are due to no growth and/or the indicated NOIP
**: False positive result (confirmed as Listeria monocytogenes while the strain was confirmed as Listeria spp with the tests of the reference method.).
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Some strains did not grow on all the plates, especially non-target strains; in these cases, the
final confirmation results were considered as negative as the confirmation procedure starts from
the streaking on selective agar plates.
No deviations wers observed except for one strain (Listeria seeligeri Ad651) which was
confirmed as Listeria monocytogenes using the MALDI Biotyper of Bruker. According to the
ISO 16140-part 6 version, this result is considered a positive deviation while the strain was
confirmed as a Listeria spp. strain (non-Listeria monocytogenes) by the tests of the ISO method.
This strain should not be considered a positive deviation but instead as a false positive result: 1
result out of 220 Listeria strains tested on 6 culture media with the MBT-smart, i.e. 0.08%. Only
agreements were observed on all the tested culture medium TSYEA, O&A, Palcam, Oxford,
MOX, and RAPID’ L.mono.
2.3.4 Evaluation
The results of the method comparison study are summarised in the Table 10 for Listeria spp.
and Table 11 for Listeria monocytogenes.
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Table 10 - Evaluation of the method comparison study results Listeria spp
Media
MALDI System Study AL ID ID≤ AL ED ED≤AL Evaluation
tested
Inclusivity 1 0 0≤1 Not applicable Not applicable Accepted

TSYEA
Exclusivity 2 Not applicable Not applicable 0 0≤2 Accepted
O&A
Palcam
Oxford
MOX
RAPID'L.mo Inclusivity 1 0 0≤1 Not applicable Not applicable Accepted
no Exclusivity 2 Not applicable Not applicable 0 0≤2 Accepted
TSYEA
O&A
Palcam
Oxford
MOX
RAPID'L.mo Inclusivity 1 0 0≤1 Not applicable Not applicable Accepted
no Exclusivity 2 Not applicable Not applicable 0 0≤2 Accepted
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Table 11 - Evaluation of the method comparison study results Listeria monocytogenes
MALDI System Media tested Study AL ID ID≤ AL ED ED≤AL Evaluation

TSYEA
O&A
Palcam
Oxford
MOX
RAPID'L.mono
TSYEA
O&A
Palcam
Oxford
MOX
RAPID'L.mono
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Listeria spp. inclusivity panel:
215 Listeria strains from the typical Listeria species, i.e. L. grayi, L. innocua, L. ivanovii, L. monocytoegenes, L. seeligeri, L. welshimeri, were
tested on 6 culture media; 100 L. monocytogenes strains and 115 other Listeria species.
3 L. grayi and 2. L. seeligeri strains were not able to recover on Palcam, 4 L. grayi strains on MOX, 11 L. grayi and 2 L. seeligeri strains on
Rapid’L. mono. 1266 identification results out of 1266 were correct at the species level with the MBT, providing 100% correct identification
rate.
Within one series of analyses dedicated to the MBT smart measurement, the quality of the spectra was not assessed by the subtyping module
to enable an identification result for some L. ivanovii or L. seeligeri strains and sub-cultures on the non-selective medium and were required to
get identification as described in the Quick Guide.
This event happened for 0.71% of the measurments (9 tests out of 1266 measurements). As previously stated, one L. seeligeri strain isolated
on Oxford was misidentified to L. monocytogenes, leading to 0.08% misidentification rate. At least, 99.23% of correct identification rate at the
species level were generated with MBT-smart. Within the set of MBT 96 biotargets (50% of the overall study), 0.79% of data showed
identification at the genus only and one misidentification at the species level that were analyzed after 24 hours shipment minimum.
As expected in the Listeria spp inclusivity panel, some newly described Listeria species or sub-species out of 9 strains were not identified: 3
strains (MBT) and 5 strains (MBT smart) (Listeria coloradensis ATCC BAA-2414, Listeria fleishmanii FSL S10-1203, Listeria floridensis FSL
S10-1187, Listeria riparia FSL S10-1204 and Listeria rocourtiae FSL F6-920), providing a “No identification possible” result. The 6903 Library
contains only one reference profile for two newly described species, i.e. L. fleischmannii and L. weihenstephanensis. The tested L.
weihenstephanensis strain was correctly identified from all the tested culture media. The tested L. fleischmannii strain was never identified;
the reference spectrum of the library and the spectra generated during the study were thus different. L. coloradensis, L. floridensis, L.
cornellensis, L. grandensis, L. riparia, L. rocourtiae were identified to Listeria spp with yellow scores when they were able to recover on the
culture media. As expected, the tested L. marthii strain was identified to L. monocytogenes as in the Quick Guide for Food.
There are not many strains of these newly described Listeria species currently available and it will be difficult to extend the subtyping concept
to get a species identification. The only approach that can be developed currently would be to increase the numbers of reference profiles
related to the species in the library. This will be completed with future updates. These species are mainly encountered in environmental
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ecosystems1,2 and are not considered in the FDA/BAM-Chapter 10 and USDA/FSIS-MLG 8.10. Only a few of them are mentioned in the ISO
11290, i.e. L. fleischmanii, L. marthii, L. rocourtiae, L. weihenstephanensis.
Listeria spp. exclusivity panel:

107 non-Listeria strains were tested; 107 strains were recovered on TSYEA, 9 on O&A, 12 on Palcam, 23 on Oxford, 8 on MOX, and 11 on
RAPID’L. mono. A total of 170 tests was run.
Only two bacterial strains were not identified (Cryptococcus albidus ATCC 34140) with either MBT and MBT-smart. 2 additional Bacillus
strains were also not identified with the MBT smart (Bacillus coagulans Ad732, Bacillus coagulans Ad1281). Bruker suggests using very fresh
cultures to avoid getting a large number of spores. This was highlighted during the training and in the report. This will be restated in the Quick
Guide by stating: “Using very fresh cultures of supposed spore-forming bacteria, i.e. less than 24 hours incubation time, usually improves the
quality of the spectra (score)”. At least, 99.41% of the tests provided an identification result with the MBT and 98.24% with the MBT-smart.
There was an observed difference between the set of MBT Biotargets 96 that was shipped prior to analysis.
For 4 strains (Carnobacterium gallinarum ATCC 49517, Kurtia gobsonii ATCC 43195, Kurthia zopfii ATCC 10538 and Zyggosaccharomyces
bailii ATCC 36945), no peak was observed using either MBT or MBT smart. For these strains, the extraction procedure was not applied while
it is typically required for yeast analyses.
None of these strains, with “NOIP” or “no peak” results, showed characteristic colonies on the tested selective agars and will be thus not be
tested in routine testing as presumptive Listeria colonies.
Disagreements:
Some disagreements were observed between the original identification of the strains obtained with the conventional identification procedures,
and the MBT. These strains were sometimes identified using 16S rDNA sequencing.
The Table 12 gathers all the disagreements observed:
In 2 cases, the misidentification is due to a lack of representative of the species in the MBT library;
- In one case, a disagreement is observed between the MALDI Biotyper and the original identification (Listeria seeligeri Ad651);
1 Graves et al, 2010. International Journal of Systematic and Evolutionary Microbiology. 60, 1280–1288
2 Linke et al, 2014. Applied and Environmental Microbiology. 80, 18 5583-5592
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- One Listeria spp strain was originally misendentified with the ISO method.
- In 7 cases, the MALDI Biotyper and the 16S rDNA sequencing give the same identification;
- In 2 cases, the genus identification is confirmed by the MALDI Biotyper;
- For one strain (Lysinibacillus sphaericus Ad724), the MALDI Biotyper identification gives sometimes Lysinibacillus spp and sometimes
Bacillus spp, but no false positive confirmation result is obtained;
- It is difficult to conclude in 2 cases as all the methods give different results.
This table gives additional information, not within the scope of the validation.
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Table 12. Disagreements observed in the test
Agreement between two methods Correct in some cases3 Disagreement with the two other methods Not possible to conclude
IS
Identification with
O
Original Identification with the MALDI
con
Study identification of 16S rDNA Biotyper and Conclusion
fir-
the strains sequencing MALDI Biotyper
mat
smart
ion
+ Listeria marthii cannot be differentiated from Listeria
Listeria marthii
Listeria monocytogenes as highlighted in the Quick Guide. However, L.
ATCC BAA- /
monocytogenes marthii doesn’t show charactersictic colonies on O&A and
1595
Listeria RAPID’L. mono
spp + Listeria
Listeria This strain does not show halo on Ottaviani & Agosti Agar. The
inclusivit seeligeri /
monocytogenes identification was confirmed by API gallery.
y panel Ad651
+ Listeria
The strain was identified to L. innocua by running a second
welshimeri Ad Listeria innocua
biochemical gallery.
1231
- Enterococcus
Enterococcus Enterococcus Enterococcus malodoratus is confirmed with the MALDI Biotyper
avium
malodoratus malodoratus and 16S rDNA sequencing.
Ad183
- Enterococcus Enterococcus The genus identification was confirmed by the MALDI Biotyper,
Enterococcus
durans faecium/ Maldi biotyper smart and 16S rDNA sequencing. It looks quite
faecium
Ad181 hirae/durans difficult to conclude for the species.
- Enterococcus Enterococcus
Enterococcus The genus identification was confirmed by the he MALDI Biotyper,
Listeria gallinarum casseliflavus/
casseliflavus Maldi biotyper smart and 16S rDNA sequencing.
spp Ad1145 gallinarum
exclusivit - Lactococcus
Leuconostoc Leuconostoc Leuconostoc mesenteroides is confirmed with the MALDI
y panel casei
mesenteroides mesenteroides Biotyper, Maldi biotyper smart and 16S rDNA sequencing.
Ad469
- Leuconostoc
Enterococcus Difficult to conclude as three identifications were obtained. But
citreum Weissella spp.
faecalis Leuconosctoc and Weisella are closely related genus.
Ad604
- Lysinibacillus
Lysinibacillus Lysinibacillus spp The genus identification was confirmed by the MALDI Biotyper,
sphaericus
fusiformis or Bacillus spp Maldi biotyper smart and 16S rDNA sequencing.
Ad724
- Staphylococcus
Micrococcus Micrococcus luteusis confirmed with the MALDI Biotyper, Maldi
epidermis Micrococcus luteus
luteus biotyper smart and 16S rDNA sequencing.
Ad931
- Staphylococcus
Staphylococcus
intermedius/ Staphylococcus The genus identification was confirmed by the he MALDI Biotyper,
Listeria intermedius
delphini/pseudinter pseudintermedius Maldi biotyper smart and 16S rDNA sequencing.
spp CIP8177
medius
exclusivit
- Enterobacter Shigella,
y panel
cancerogenus Escherichia, Escherichia coli Difficult to conclude
QL11010.1 Enterobacter
- Lysinibacillus Lysinibacillus
The genus identification was confirmed by the MALDI Biotyper
sphaericus / fusiformis
and the Maldi biotyper smart.
ATCC BAA-378 or boronitolerans
= Enterococcus Enterococcus
Enterococcus The genus identification was confirmed by the MALDI Biotyper,
gallinarum casseliflavus/
Listeria casseliflavus Maldi biotyper smart and 16S rDNA sequencing.
Ad1145 gallinarum
monocyt
= Lysinibacillus
ogenes Lysinibacillus Lysinibacillus spp The genus identification was confirmed by the MALDI Biotyper,
sphaericus
fusiformis or Bacillus spp Maldi biotyper smart and 16S rDNA sequencing.
Ad724
exclusivit
= Lysinibacillus Lysinibacillus
y panel The genus identification was confirmed by the MALDI Biotyper
sphaericus / fusiformis
and the Maldi biotyper smart.
ATCC BAA-378 or boronitolerans
.
3 The MALDI-Biotyper gives an identification in agreement with the expected result for some plates.
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4. Conclusions Method Comparison Study
Listeria spp.
For inclusivity, the AL was met for both MBT Systems, and for all the tested media, as the
calculated values for ID are lower than the AL fixed respectively at 1 for 150 target strains
tested.
For exclusivity, the AL was met for both MBT Systems, and for all the tested media, as
the calculated values for ED are lower than the AL fixed respectively at 2 for 100 non-
target strains
Listeria moncytogenes
For inclusivity, the AL is met for both MBT Systems, and for all the tested media, as
the calculated values for ID are lower than the AL fixed respectively at 2 for 100
target strains tested.
For exclusivity, the AL is met for both MBT Systems, and for all the tested media, as
the calculated values for ED are lower than the AL fixed respectively at 2 for 100 non-
target strains.
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5 Interlaboratory Study
Protocol and Materials
A total of 16 participants from 16 laboratories participated in this study. A detailed list

of participants is presented in Annex M. Each collaborator received 16 target strains
and 8 non-target strains. The strains were blinded coded as follows (See Table 13).
The list of target and non-target strains tested is provided in Annex N.
Microbeads were thawed and streaked on nutrient agar to check the purity of the
strains and then subcultured in conservation agar media for shipment. Upon receipt,
the samples were plotted on targets or biotargets depending on the collaborators.
Only one collaborator (N) has a MBT smart with the microflex LT/SH smart
instrument since this is a new instrument. All the other collaborators have a MBT
with the microflex LT/SH instrument.
Table 131. Targets used by the collaborators and the expert lab.
Collaborators Type of target used
A Reusable polished steel target
B Disposable Biotarget 96
C Reusable polished steel target
D Reusable polished steel target
E Reusable polished steel target
F Disposable Biotarget 96
G Reusable polished steel target
H Disposable Biotarget 96
I Reusable polished steel target
J Disposable Biotarget 96
K Reusable polished steel target
L Disposable Biotarget 96
M Disposable Biotarget 96
N Disposable Biotarget 96
O Disposable Biotarget 96
P Reusable polished steel target
Q (ADRIA) Disposable Biotarget 96
Collaborators Total Reusable Polished Steel Targets 8
Total Disposable Biotargets 8
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Transport
Isolates were shipped on Tuesday, August 22nd, 2017. Packages were sent via TNT
and transported considering the transport safety requirements (Biological
substances: category B) according to IATA4 tubes. UN3373 packages were used
and included a primary receptacle that included the strain and a second packaging
that fully encapsulated the primary receptable. The package receipt data as well as
the analysis date are indicated in Table 14. All packages were delivered in good
conditions to the collaborators.
Table 14 - Package receipt and analysis date

Collaborators Receipt date Analysis date
A 08/24/2017 08/28/2017
B 08/24/2017 08/29/2017
C 08/23/2017 08/28/2017
D 08/24/2017 08/28/2017
E 08/24/2017 08/28/2017
F 0824/2017 08/28/2017
G 08/23/2017 08/28/2017
H 08/23/2017 08/23/2017
I 08/23/2017 08/28/2017
J 08/23/2017 08/28/2017
K 08/23/2017 08/28/2017
L 08/23/2017 08/28/2017
M 08/25/2017 08/28/2017
N 08/25/2017 30/08/2017
O 08/23/2017 08/28/2017
P 08/23/2017 08/28/2017
Analysis
The collaborators and the Expert Laboratory tested the blind coded strains as
described in Annex O. Each collaborator streaked the blind coded targets and non-
target strains onto a non-selective (TSYEA) and selective agar plates before applying
the alternative MALDI-TOF Confirmation Procedure (O&A, Oxford and
RAPID’L.mono). The MOX and Palcam plates were not tested for the inter-laboratory
study As stated in the project, if all the data were correct on all the tested selective
agars in the Method Comparison Study, only selected selective agars will be tested,
i.e. the standard O&A formulation and one non chromogenic standard formulation, as
well as the proprietary chromogenic, i.e. RAPID’L. mono. All the strains were also
tested using the ISO standard procedures (catalase, gram, haemolysis, motility,
biochemical tests) from the non-selective agar plate (TSYEA).
Statistics and Results
The data from 16 collaborators were received; the raw data are provided in Annex P.
4 IATA : International Air Transport Association
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Within the sets of data retained for the interpretation, 100% correct identification rate
was obtained with the MBT for the tested Listeria strains. 3 misidentifications at the
Listeria species level were observed with the ISO 11290 procedure and it was not
possible to discriminate between L. seeligeri and L. ivanovii in 10 cases or to identify
clearly one L. grayi isolate; this corresponds to 3.57% (13 out of 364) of the overall
measurements.
For the Gram positive non-Listeria (exclusivity) organisms, 100% correct identification
rate was observed with the MBT. The MALDI Biotyper method identified Bacillus
thuringiensis as a member of the Bacillus cereus group with no possible identification
to the species level, as described in the ISO 7932 standard; this is clearly highlighted
in the MALDI Biotyper result report.
Using the conventional identification procedure with biochemical galleries, 3

collaborators identified B. pumilus as L. grayi and one collaborator identified B.
thuringiensis as L. ivanovii; this corresponds to 3.85% (4 out of 104) of
misidentification, as well false confirmation to Listeria spp in the overall
measurements.
A summary of the inconsistent results or problems encountered during the study are
provided in Table 15 for Listeria spp and Table 16 for Listeria monocytogenes.
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Table 15. Summary of the inconsistent results or problems encountered during
the study by the collaborators - Listeria spp
Comments Collaborator
Collabo-
retained for
rator Reference method Alternative method
interpretation
A No comment No comment Yes
B No comment No comment Yes
C No comment No comment Yes
D No comment No comment Yes
E No comment No comment Yes
▪ Impossible to conclude for ▪ One strain did not grow on
some strains due to doubtful TSYEA
identification by the
F No
biochemical tests.
▪ One strain did not grow on
TSYEA.
G No comment No comment Yes
Bad identification for 2 strains by No comment
H Yes
the biochemical tests.
▪ Oxford media was not tested
I Inconsistent Gram stain result Yes
by the collaborator
J No comment No comment Yes
▪ The collaborator did not follow ▪ The collaborator did not follow
the entire workflow. the entire workflow.
▪ Misidentifications were ▪ Some cross contaminations
observed for several were observed.
K No
biochemical test results.
▪ Inconsistent results were
encountered for the Gram
stain and haemolysis results.
L No comment No comment Yes
M No comment No comment Yes
▪ Inconsistent results for some ▪ The subtyping module did not
biochemical tests proceed correctly in several
N Yes
cases.
▪ Some results are missing.
O No comment No comment Yes
▪ No biochemical test result for
one strain
P ▪ Many inconsistent results were No comment Yes
observed for the haemolysis
and Gram stain tests.
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Table 26. Summary of the inconsistent results or problems encountered
during the study by the collaborators - Listeria monocytogenes
Comments Collaborator
Collabo-
retained for
rator Reference method Alternative method
interpretation
A No comment No comment Yes
B No comment No comment Yes
C No comment No comment Yes
D No comment No comment Yes
E No comment No comment Yes
▪ Impossible to conclude for some ▪ One strain did not grow on
strains due to a double TSYEA
identification by the biochemical ▪ The collaborator did not follow
F tests. the entire workflow for one strain No
▪ One strain did not grow on on Oxford.
TSYEA. ▪ Positive deviation was observed
on Oxford for one strain.
G No comment No comment Yes
H No comment No comment Yes
▪ Oxford media was not tested by
the collaborator. Yes, except for
▪ 2 inconsistent haemolysis
I ▪ The collaborator did not follow Oxford and
results.
the entire workflow for one strain RAPID’L.mono
on RAPID’L.mono.
J No comment No comment Yes
▪ The collaborator did not follow ▪ The collaborator did not follow
the entire workflow. the entire workflow.
▪ Misidentifications were observed ▪ Some cross contaminations
for several biochemical test were observed.
K No
results.
▪ Inconsistent results were
encountered for the Gram stain
and haemolysis results.
L No comment No comment Yes
M No comment No comment Yes
▪ Inconsistent results for some ▪ The subtyping module did not
biochemical tests and proceed correctly in several
N Yes
haemolysis cases.
▪ Some results are missing.
O No comment No comment Yes
▪ One inconsistent result was
P No comment Yes
observed for a Gram stain.
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Problems were primarily encountered using the confirmatory tests of the ISO 11290
method. Additionally, some collaborators did not follow the entire MBT workflow and
stopped with the DT, while no identification result obtained. The entire data set
provided by laboratory K was excluded, the MBT workflow was not followed and data
were missing. Collaborators F and N were also excluded due to data inconsistencies
and/or missing. The collaborators listed in Table 17 were kept for interpretation.
Table 3. List of collaborators kept for interpretation per media used for
streaking
TSYEA O&A Oxford RAPID’L.mono
Collabo-
L.mono- L.mono- L.mono- L.mono-
rators L. spp L. spp L. spp L. spp
cytogenes cytogenes cytogenes cytogenes
A Yes Yes Yes Yes Yes Yes Yes Yes
B Yes Yes Yes Yes Yes Yes Yes Yes
C Yes Yes Yes Yes Yes Yes Yes Yes
D Yes Yes Yes Yes Yes Yes Yes Yes
E Yes Yes Yes Yes Yes Yes Yes Yes
F No No No No No No No No
G Yes Yes Yes Yes Yes Yes Yes Yes
H Yes Yes Yes Yes Yes Yes Yes Yes
I Yes Yes Yes Yes No No Yes Yes
J Yes Yes Yes Yes Yes Yes Yes Yes
K No No No No No No No No
L Yes Yes Yes Yes Yes Yes Yes Yes
M Yes Yes Yes Yes Yes Yes Yes Yes
N Yes Yes Yes Yes Yes Yes Yes Yes
O Yes Yes Yes Yes Yes Yes Yes Yes
P Yes Yes Yes Yes Yes Yes Yes Yes
TOTAL 13 13 13 13 12 12 13 12
A summary of the results kept for interpretation are given in the following tables:
TSYEA O&A Oxford RAPID’L.mono

For Listeria spp. Table 18 Table 19 Table 20 Table 21
For Listeria monocytogenes Table 22 Table 23 Table 24 Table 25
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Table 18. Summary of the results obtained for TSYEA - Listeria spp
TSYEA
Number of correctly confirmed Number of correctly non-confirmed
strains in the inclusivity part strains in the exclusivity part
Collaborators (PA) (NA)
Alternative confirmation method Alternative confirmation method
A 16/16 8/8
B 16/16 8/8
C 16/16 8/8
D 16/16 8/8
E 16/16 8/8
G 16/16 8/8
H 16/16 8/8
I 16/16 8/8
J 16/16 8/8
L 16/16 8/8
M 16/16 8/8
O 16/16 8/8
P 16/16 8/8
TOTAL (13 labs) 208/208 104/104
Table 19. Summary of the results obtained for O&A - Listeria spp
O&A
A 16/16 8/8 (including 4 NA no growth)
B 16/16 8/8 (including 7 NA no growth)
C 16/16 8/8 (including 7 NA no growth)
D 16/16 8/8 (including 4 NA no growth)
E 16/16 8/8 (including 5 NA no growth)
G 16/16 8/8 (including 8 NA no growth)
H 16/16 8/8 (including 6 NA no growth)
I 16/16 8/8 (including 8 NA no growth)
J 16/16 8/8 (including 7 NA no growth)
L 16/16 8/8 (including 8 NA no growth)
M 16/16 8/8 (including 5 NA no growth)
O 16/16 8/8 (including 6 NA no growth)
P 16/16 8/8 (including 5 NA no growth)
13 labs 208/208 104/104
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Table 20. Summary of the results obtained for Oxford - Listeria spp
strains in the inclusivity part (PA) strains in the exclusivity part (NA)
Collaborators5
Alternative confirmation Alternative confirmation procedure
procedure
Collaborator 1 16/16 8/8 (7 with no growth)
Total 240/240 120/120
15 Collaborators 240/240 120/120
Table 21. Summary of the results obtained for RAPID’L.mono - Listeria spp
RAPID'L.mono
TOTAL (13 labs) 208/208 104/104
5Data from 2 collaborators were excluded from analysis due to technical deviations from the test protocol or non-viable
growth was obtained.
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Table 22. Summary of the results obtained for TSYEA - Listeria monocytogenes
TSYEA
Number of correctly confirmed strains in Number of correctly non-confirmed strains
the inclusivity part in the exclusivity part
A 16/16 8/8
B 16/16 8/8
C 16/16 8/8
D 16/16 8/8
E 16/16 8/8
G 16/16 8/8
H 16/16 8/8
I 16/16 8/8
J 16/16 8/8
L 16/16 8/8
M 16/16 8/8
O 16/16 8/8
P 16/16 8/8
TOTAL (13 labs) 208/208 104/104
Table 23. Summary of the results obtained for O&A - Listeria monocytogenes
O&A
TOTAL (13 labs) 208/208 104/104
37/130
Table 24. Summary of the results obtained for Oxford - Listeria
monocytogenes
Oxford
TOTAL (12 labs) 192/192 96/96
Table 25. Summary of the results obtained for RAPID’L.mono - Listeria

monocytogenes
RAPID'L.mono
TOTAL (12 labs) 192/192 96/96
38/130
The results of the interlaboratory study are summarised in Table 26 for Listeria spp
and Table 27 for Listeria monocytogenes.
Table 26. Summary of the results in the interlaboratory study - Listeria spp.
Number
Media of
Study N IA ID EA ED
tested labs
Not Not
13 Inclusivity 208 208 0
Applicable Applicable
TSYEA
Not Not
13 Exclusivity 208 104 0
Not Not
O&A
Not Not
Not Not
Oxford
Not Not
Not Not
RAPID'L Applicable Applicable
.mono Not Not
Table 27. Summary of the results in the interlaboratory study - Listeria

monocytogenes
Number
of
Media tested Study N IA ID EA ED
labs
Not Not
TSYEA
Not Not
Not Not
O&A
Not Not
Not Not
Oxford
Not Not
Not Not
RAPID'L.mon Applicable Applicable
o Not Not
The results evaluated according to the acceptability limits (AL) are given in Table 28
for Listeria spp and Table 29 for Listeria monocytogenes.
39/130
Table 28. Evaluation of the interlaboratory study results - Listeria spp.
Media Number A Evaluatio
Study N ID ID≤AL ED ED≤AL
tested of labs L n
Inclusivit 20 Not Not
13 3 0 0≤3 Accepted
y 8 Applicable Applicable
TSYEA
Exclusivit 20 Not Not
O&A
Exclusivit 10 Not Not
Oxford
Exclusivit Not Not
12 96 2 0 0≤2 Accepted
y Applicable Applicable
RAPID'L. y 8 Applicable Applicable
mono Exclusivit 10 Not Not
Table 29. Evaluation of the interlaboratory study results - Listeria

monocytogenes
Media Number A Evaluat
Study N ID ID≤AL ED ED≤AL
tested of labs L ion
Inclusiv 20 Not Not Accept
13 3 0 0≤3
ity 8 Applicable Applicable ed
TSYEA
Exclusi 10 Not Not Accept
13 3 0 0≤3
vity 4 Applicable Applicable ed
13 3 0 0≤3
O&A
Exclusi 10 Not Not Accept
13 3 0 0≤3
vity 4 Applicable Applicable ed
12 2 0 0≤2
Oxford
Exclusi Not Not Accept
12 96 2 0 0≤2
vity Applicable Applicable ed
13 3 0≤3
RAPID'L.m ity 8 0 Applicable Applicable ed
ono Exclusi Not Not Accept
13 96 3 0 0≤3
vity Applicable Applicable ed
Eight collaborators used the Reusable polished steel targets and eight collaborators
used the Disposable targets; no difference was observed between both targets,
confirming the reliability of the method.
6 Conclusions of Interlaboratory Study
Listeria spp.
The AL is met for the four media tested during this inter-laboratory study (non-
selective agar, O&A, Oxford and RAPID’L.mono), as the calculated values for ND -
PD and ND + PD for the inclusivity are lower than the ALs, as well as the calculated
values for ND + PD for the exclusivity study.
40/130
Listeria monocytogenes
The AL is met for the four media tested during this inter-laboratory study (non-
selective agar, O&A, Oxford and RAPID’L.mono), as the calculated values for ND -
PD and ND + PD for the inclusivity are lower than the ALs, as well as the calculated
values for ND + PD for the exclusivity study.
41/130
Annex A - Flow diagrams of the reference methods
ISO 11290-1 (May 2017): Microbiology of the food chain -

Horizontal method for the detection and enumeration of Listeria
monocytogenes and of Listeria spp. – Part 1: detection method
Test portion (25 g or 25 ml)


Primary enrichment medium (half Fraser broth) (225 g or 225 m)

Incubation at 30°C ± 24 h to 26 h
0.1 ml of culture
in 10 ml of secondary
enrichment medium (Fraser broth)
Incubation at 37°C ± 1°C

for 24 h ± 2 h
Plating out on Agar Listeria according to

Ottaviani and Agosti and second selective medium

Incubation of Agar Listeria according to
Ottaviani and Agosti for 24 h ± 2 h and an additional 24 h ± 2 h at 37°C ± 1°C
Incubation of second selective medium according to the chosen medium as specified by the manufacturer

Confirmation of
L. monocytogenes Listeria spp.
Gram Gram
Haemolysis Catalase
L-Rhamnose
D-Xylose
42/130
ISO 11290-2 (May 2017): Microbiology of the food chain -
Horizontal method for the detection and enumeration of Listeria
monocytogenes and of Listeria spp. - Part 2: enumeration method
Test portion (x g or x ml)


Diluent for the preparation of the initial suspension (9 x g or 9 x ml)

Plate 2 x1 ml on 3 plates for the first dilution and plate 0.1 ml of each dilution onto Ottaviani and Agosti
agar

Incubation for 24 h ± 2 h and an additional 24 h ± 2 h at 37°C ± 1°C

Enumeration of presumptive Listeria monocytogenes and/or Listeria spp.

Confirmation of
L. monocytogenes Listeria spp.
Haemolysis Gram
L-Rhamnose Catalase
D-Xylose
43/130
Annex B - Flow diagram of the alternative method
44/130
Isolated colonies on non-selective culture medium, TSYEA,
O&A, Palcam, Oxford, MOX and RAPID’L. mono
 
 Extraction procedure 

300 µl deionized water into an Eppendorf tube

Add 1 colony to test, mix thoroughly

Add 900 µl EtOH, mix thoroughly

Centrifuge at maximum speed (1300 to 1500 rpm) for 2 min.
Decant supernatant, centrifuge again and remove EtOH

Allow EtOH pellet to dry at room temperature for at least 5 min

Add 70 % formic acid (1 to 80 µl depending on the size of the pellet)
to the pellet and mix

Add pure acetonitrile (1 to 80 µl depending on the size of the pellet)
and mix carefully

Direct transfer Centrifuge 2 min. Extended direct
at maximum speed
procedure transfer procedure
Overlay the spot with 1 µl of 70 %

Formic Acid and allow to dry at
room temperature
Pipette 1 µl of supernatant or 1 colony (for the Direct Transfer Procedure and the Extended direct
transfer procedure) onto a MALDI target plate
and allow it to dry at room temperature
Overlay the entire spot with 1 µl HCCA solution

within 1 hour and allow it to dry at room temperature

MALDI-TOF
The Direct Transfer (DT) Sample preparation can identify 90%-95% of the bacterial
isolates. When no identification is obtained, this procedure should be repeated. If
the no result is repeated, then the users should run the eDT. When needed the EXT
sample preparation should be carried out. In some rare cases, a sub-culture on a
nonselective agar may be required.
45/130
Annex B - Kit inserts
46/130
47/130
48/130
49/130
50/130
51/130
52/130
53/130
54/130
Annex C – Certificate of equivalence
55/130
Annex D – Listeria spp. and Listeria monocytogenes testing
Method Comparison Study
Cryobead of already well characterized strain

TSYEA
Test on non-selective agar Test on selective agars
 
Streaking onto: BHI
TSYEA Streaking onto:
O&A
 Palcam
Oxford
Incubation for 24- 48 h at 37°C
MOX

One colony RAPID’L. mono

Incubation for 24 h - 48 h at 37°C

One colony per plate
Confirmation by using the Direct Transfer Procedure

of the Biotyper of BRUKER on fresh culture
56/130
Annex E - Raw data inclusivity study
See Excel File: 2017LR75 - Microval MCS and ILS Listeria spp and mono (Version
1).xlsx; Tab «Tab “Raw data L.spp Incl” and “Raw data L.mono Incl”»
57/130
Annex F - Raw data exclusivity study
See Excel File: 2017LR75 - Microval MCS AND ILS Listeria spp and mono (Version
1).xlsx; Tab “Raw data L.spp Excl” and “Raw data L.mono Excl”
58/130
Annex G - Results inclusivity study:
+: the strain was confirmed to be the target -: the strain was not confirmed to be the target NOIP: NO Organism Identification Possible
INCLUSIVITY - Listeria spp.

Alternative method
Target Strain LT/SH MALDI-MS System
TSYEA O&A Palcam Oxford MOX RAPID'L.mono
Alternat Alternat Alternat
Refere ive ive Alternative ive Alternative Alternative
nce Fin method method method Fin method method method
metho al confirm confirm confirmatio al confirm confirmatio confirmatio
d Final Final Final Final Final Final Final Final Final Final
Serot res ation ation n res ation n n
N Genus Species Reference Origin Lab Interpret result Interpret result Interpretati Interpret resul Interpretati result Interpretati
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
(+/- ed to ed to to identity (+/- ed to to identity to identity
) identity identity of the ) identity of the of the
of the of the strain of the strain strain
strain strain strain
Adri
1 Listeria grayi Ad1198 Smoked salmon + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
- (no
Adri NA (no NA (no
2 Listeria grayi Ad1229 Chitterling + + PA IA + PA IA + PA IA + PA IA grow + PA IA
a growth) growth)
th)
- (no
Adri - (no NA (no NA (no NA (no NA (no
3 Listeria grayi Ad1295 Spinash + + PA IA + PA IA + PA IA grow + PA IA
a growth) growth) growth) growth) growth)
th)
Adri
4 Listeria grayi Ad1296 Vegetables + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
5 Listeria grayi Ad1443 Pork fat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
- (no
Adri NA (no NA (no
6 Listeria grayi Ad1444 Ham + + PA IA + PA IA + PA IA + PA IA grow + PA IA
a growth) growth)
th)
Adri
7 Listeria grayi Ad1490 Fish and vegetable pie + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
8 Listeria grayi Ad1504 Salmon terrine + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
9 Listeria grayi Ad2064 Fermented sausage + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
- (no
Adri NA (no NA (no
10 Listeria grayi Ad2148 Rillette + + PA IA + PA IA + PA IA + PA IA grow + PA IA
a growth) growth)
th)
Adri - (no NA (no NA (no
11 Listeria grayi Ad2415 Rillette + + PA IA + PA IA + PA IA + PA IA + PA IA
a growth) growth) growth)
Adri
12 Listeria innocua Ad 1230 Seafood product + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
13 Listeria innocua Ad1231 Deli salad + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
14 Listeria innocua Ad 1675 Fish + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
15 Listeria innocua Ad 1676 RT reheat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
16 Listeria innocua Ad 1771 Ewe milk + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
17 Listeria innocua Ad2414 Pasteurized cheese + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
59/130
Alternative method
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
Adri
18 Listeria innocua Ad 643 Veal meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
19 Listeria innocua Ad 655 Brine + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
20 Listeria innocua Ad 657 Raw milk cheese (cantal) + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Raw milk cheese (Pont Adri
21 Listeria innocua Ad 661 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
L'Evêque) a
Adri
22 Listeria innocua Ad 663 Dairy environmental sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
23 Listeria innocua Ad 671 Smoked pork meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
24 Listeria ivanovii Ad1288 Ewe milk + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
25 Listeria ivanovii Ad1289 Raw milk cheese + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
26 Listeria ivanovii Ad1290 Milk powder + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
27 Listeria ivanovii Ad1292 Merguez + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
28 Listeria ivanovii Ad1308 Raw sheep meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
a
Adri
a
Adri
31 Listeria ivanovii Ad616 Dairy environmental sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
32 Listeria ivanovii Ad676 Pork cheek + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
33 Listeria ivanovii Ad677 Shrimp + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
34 Listeria ivanovii Ad678 Ewe cheese + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Raw milk cheese Adri
35 Listeria ivanovii Ad991 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
(Roquefort) a
monocytoge Adri
36 Listeria 153 Soft cheese (Munster) VI b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
37 Listeria 1972/2399 Puff pastry with mushrooms VI b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Puff pastry egg and ham Adri
38 Listeria 1973/2400 VI b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes (Quiche lorraine) a
60/130
Alternative method
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
monocytoge Adri
39 Listeria 2407/3139 Tripes with tomatoes IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
40 Listeria 32.183 Croque-Monsieur II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
41 Listeria 5721/6179 Smoked bacon IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
42 Listeria 7111/7516 Pâté (Rillettes) IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
43 Listeria 913/1 048 Black pudding IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Cooked delicatessen Adri
44 Listeria A00C040 IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes (Museau) a
monocytoge Adri
45 Listeria A00C041 Sausage La + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
46 Listeria A00C042 Raw sausage IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
47 Listeria A00C043 Smoked Bacon II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
48 Listeria A00C044 Poultry (duck) II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge RTE food (Osso bucco with Adri
49 Listeria A00C052 II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes turkey) a
monocytoge Adri
50 Listeria A00C054 Beef heart IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
51 Listeria A00M032 Smoked salmon IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
52 Listeria A00M088 Smoked salmon II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
53 Listeria Ad235 Poultry II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
54 Listeria Ad249 Environmental sample II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
55 Listeria Ad253 Hard cheese II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
56 Listeria Ad260 Semi hard cheese II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
57 Listeria Ad265 Tongue II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
58 Listeria Ad266 Poultry II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
59 Listeria Ad267 Dry sausage II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
61/130
Alternative method
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
monocytoge Adri
60 Listeria Ad268 Cured ham IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
61 Listeria Ad270 Fermented sausage IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
nes a
monocytoge Adri
63 Listeria Ad273 Cured delicatessen II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Ready-to-eat food (Asiatic Adri
64 Listeria Ad274 II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes meal) a
monocytoge Adri
65 Listeria Ad285 Green pepper La + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
monocytoge Adri
66 Listeria Ad494 Deli salad (piémontaise) II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
nes a
Adri
67 Listeria seeligeri Ad1237 Raw milk + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Smoked fish environmental Adri
68 Listeria seeligeri Ad1267 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
sample a
Adri
69 Listeria seeligeri Ad1293 Parsley + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
70 Listeria seeligeri Ad1297 Merguez + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
71 Listeria seeligeri Ad1754 Zucchini + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
a
Adri
a
Adri
74 Listeria seeligeri Ad2634 Trout + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
a
Adri
76 Listeria seeligeri Ad649 Cheese + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
77 Listeria seeligeri Ad651 Environment sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
78 Listeria seeligeri Ad652 Dairy environmental sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
a
Adri
80 Listeria seeligeri BR18 Environment sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
62/130
Alternative method
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
Adri
81 Listeria welshimeri Ad1175 Fried rize + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
82 Listeria welshimeri Ad1204 Ham + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
83 Listeria welshimeri Ad1211 Frozen ground beef meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
84 Listeria welshimeri Ad1223 Beef meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
85 Listeria welshimeri Ad1228 Turkey meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
86 Listeria welshimeri Ad1234 Sausages + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Slaughterhouse Adri
87 Listeria welshimeri Ad1262 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
environment sample a
sample a
Adri
89 Listeria welshimeri Ad1667 Raw milk cheese + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
90 Listeria welshimeri Ad1670 Rosette + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
91 Listeria welshimeri Ad1671 Smoked sausage + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Q
ATCC NO No No
92 Listeria coloradensis Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA
BAA-2414 IP result result
s
Q
FSL F6- - (no NA (no NA (no
93 Listeria cornellensis Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA
0969 growth) growth) growth)
s
Q
FSL S10- NO No No NO No No NOI
94 Listeria fleischmannii Not Available Lab + + PA IA + PA IA No result No result NOIP No result No result
1203 IP result result IP result result P
s
Q
FSL S10- NO No No NOI No No NO No No NOI
95 Listeria floridensis Not Available Lab + NOIP No result No result No result No result NOIP No result No result
1187 IP result result P result result IP result result P
s
Q
FSL F6- No No No No NO No No
96 Listeria grandensis Not Available Lab + + PA IA No result No result + PA IA + PA IA
0971 peak result result peak IP result result
s
Q
ATCC - (no NA (no NA (no
97 Listeria grayi Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA
s
Q
NCTC - (no NA (no NA (no
s
63/130
Alternative method
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
Q
QL - (no NA (no NA (no
99 Listeria grayi Industry Lab + + PA IA + PA IA + PA IA + PA IA + PA IA
030911-12 growth) growth) growth)
s
Q
10 QL - (no NA (no NA (no
Listeria grayi Unknown Lab + + PA IA + PA IA + PA IA + PA IA + PA IA
0 051111-1 growth) growth) growth)
s
Q
s
Q
s
Q
s
Q
s
Q
s
Q
s
Q
10 UVM - (no NA (no NA (no - (no NA (no NA (no
Listeria grayi Not Available Lab + + PA IA + PA IA + PA IA + PA IA
7 LGRA001 growth) growth) growth) growth) growth) growth)
s
Q
10 ATCC
Listeria innocua Cow brain Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
8 33090
s
Q
10 QL
Listeria innocua Fish Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
9 030911-1
s
Q
11 QL
0 030911-2
s
Q
11 QL
Listeria innocua Seasoning Powder Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
1 32811.1
s
Q
11 QL
2 32811.2
s
64/130
Alternative method
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
Q
11 QL
3 32811.3
s
Q
11
Listeria innocua UVM 11/2 Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
4
s
Q
11
Listeria innocua UVM 167 Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
5
s
Q
11
6
s
Q
11
7
s
Q
11
8
s
Q
11
9
s
Q
12
0
s
Q
12
1
s
Q
12
2
s
Q
12
3
s
Q
12 ATCC
Listeria ivanovii Washing water Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
4 BAA-139
s
Q
12 ATCC
Listeria ivanovii Sheep fetus Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
5 BAA-678
s
Q
12 QL
Listeria ivanovii Clinical Isolate Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
6 030911-10
s
65/130
Alternative method
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
Q
12 QL
Listeria ivanovii Clinical Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
7 030911-11
s
Q
12 UVM
Listeria ivanovii Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
8 Liv004
s
Q
12 UVM
9 Liv005
s
Q
13 UVM
0 Liv006
s
Q
13 QL
Listeria londoniensis Clinical Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
1 030911-9
s
Q
13 ATCC
Listeria marthii Soil Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
2 BAA-1595
s
Q
13 FSL S10- NO No No NOI No No NO No No NOI
Listeria riparia Not Available Lab + NOIP No result No result No result No result NOIP No result No result
3 1204 IP result result P result result IP result result P
s
Q
13 FSL F6- NOI No No
Listeria rocourtiae Not Available Lab + + PA IA + PA IA + PA IA + PA IA NOIP No result No result
4 0920 P result result
s
Q
13 ATCC
Listeria seeligeri Human feces Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
5 11289
s
Q
13 ATCC - (no NA (no NA (no - (no NA (no NA (no
Listeria seeligeri Not Available Lab + + PA IA + PA IA + PA IA + PA IA
6 11856 growth) growth) growth) growth) growth) growth)
s
Q
s
Q
13 ATCC Intesti'/l content of
Listeria seeligeri Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
8 51334 Clethrionomys glareolus
s
Q
13 ATCC
Listeria seeligeri Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
9 51335
s
Q
14 FSL-S4-
0 035
s
66/130
Alternative method
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
Q
14 QL
Listeria seeligeri Creamer Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
1 031011-1
s
Q
14 QL
2 031011-2
s
Q
14 QL
3 031011-5
s
Q
14 QL
4 030911-6
s
Q
14 weihensteph FSL R9- - (no NA (no NA (no
Listeria Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA
5 enensis 0317 growth) growth) growth)
s
Q
14 ATCC
Listeria welshimeri Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
6 43548
s
Q
14 ATCC
Listeria welshimeri Soil Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
7 43549
s
Q
14 ATCC
Listeria welshimeri Human feces Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
8 43550
s
Q
14 ATCC
9 43551
s
Q
15 QL
Listeria welshimeri Beef Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
0 030911-7
s
Q
15 QL
1 030911-8
s
Q
15 QL
Listeria welshimeri Unknown Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
2 040713.1
s
Q
15 UVM
3 LW001
s
Q
15 UVM
4 LW002
s
67/130
Alternative method
ype ult compar
ation (+/-)
compar
ation (+/-)
compared
on
ult compar
ation t (+/-)
compared
on (+/-)
compared
on
Q
15 UVM
5 LW003
s
N 155 155 155 155 155 155
PA 152 151 147 150 148 136
ND 0 0 0 0 0 0
Tot NA 0 0 0 0 0 0
al NA (no
0 0 5 0 4 15
growth)
PD 0 0 0 0 0 0
No result 3 4 3 5 3 4
68/130
Alternative method
Target Strain LT/SH MALDI-smart System
Ser Refere Fin ive Fin ive Alternative Fin ive Alternative Alternative
nce Final
o- al method al method method al method method method
metho confirm confirm confirmatio confirm confirmatio confirmatio
d Final Final Final Final Final Final Final Final
typ res ation ation n ation n n
N° Genus Species Reference Origin Lab compar
Interpret res compar
Interpret result compared
Interpretati res compar
Interpret result
compared
Interpretati result (+/-
compared
Interpretati
e ult ation ation on ation (+/-) on ) on
ed to ult ed to to identity ult ed to to identity to identity
identity identity of the identity of the of the
(+/- (+/- (+/-
of the of the (+/-) strain of the strain strain
) ) )
Adri
1 Listeria grayi Ad1198 Smoked salmon + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
-
Adri (no NA (no NA (no
2 Listeria grayi Ad1229 Chitterling + + PA IA + PA IA + PA IA + PA IA + PA IA
a grow growth) growth)
th)
-
Adri - (no NA (no NA (no (no NA (no NA (no
3 Listeria grayi Ad1295 Spinash + + PA IA + PA IA + PA IA + PA IA
a growth) growth) growth) grow growth) growth)
th)
Adri
4 Listeria grayi Ad1296 Vegetables + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
5 Listeria grayi Ad1443 Pork fat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
-
6 Listeria grayi Ad1444 Ham + + PA IA + PA IA + PA IA + PA IA + PA IA
th)
Adri
7 Listeria grayi Ad1490 Fish and vegetable pie + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
8 Listeria grayi Ad1504 Salmon terrine + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
9 Listeria grayi Ad2064 Fermented sausage + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
-
th)
Adri - (no NA (no NA (no
a growth) growth) growth)
Adri
12 Listeria innocua Ad 1230 Seafood product + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
13 Listeria innocua Ad1231 Deli salad + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
14 Listeria innocua Ad 1675 Fish + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
15 Listeria innocua Ad 1676 RT reheat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
16 Listeria innocua Ad 1771 Ewe milk + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
17 Listeria innocua Ad2414 Pasteurized cheese + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
69/130
Alternative method
nce Final
Interpret result
compared
compared
Interpretati
(+/- (+/- (+/-
) ) )
Adri
18 Listeria innocua Ad 643 Veal meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
19 Listeria innocua Ad 655 Brine + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
20 Listeria innocua Ad 657 Raw milk cheese (cantal) + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Raw milk cheese (Pont Adri
21 Listeria innocua Ad 661 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
L'Evêque) a
Adri
22 Listeria innocua Ad 663 Dairy environmental sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
23 Listeria innocua Ad 671 Smoked pork meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
a
Adri
a
Adri
26 Listeria ivanovii Ad1290 Milk powder + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
27 Listeria ivanovii Ad1292 Merguez + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
28 Listeria ivanovii Ad1308 Raw sheep meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
a
Adri
a
Adri
31 Listeria ivanovii Ad616 Dairy environmental sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
32 Listeria ivanovii Ad676 Pork cheek + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
33 Listeria ivanovii Ad677 Shrimp + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
34 Listeria ivanovii Ad678 Ewe cheese + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Raw milk cheese Adri
35 Listeria ivanovii Ad991 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
(Roquefort) a
monocytogen VI Adri
36 Listeria 153 Soft cheese (Munster) + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen VI Adri
37 Listeria 1972/2399 Puff pastry with mushrooms + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen Puff pastry egg and ham VI Adri
38 Listeria 1973/2400 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es (Quiche lorraine) b a
70/130
Alternative method
nce Final
Interpret result
compared
compared
Interpretati
(+/- (+/- (+/-
) ) )
monocytogen IV Adri
39 Listeria 2407/3139 Tripes with tomatoes + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen Adri
es a
monocytogen IV Adri
41 Listeria 5721/6179 Smoked bacon + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen IV Adri
42 Listeria 7111/7516 Pâté (Rillettes) + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen IV Adri
43 Listeria 913/1 048 Black pudding + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen Cooked delicatessen IV Adri
44 Listeria A00C040 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es (Museau) b a
monocytogen Adri
45 Listeria A00C041 Sausage La + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es a
monocytogen IV Adri
46 Listeria A00C042 Raw sausage + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen Adri
47 Listeria A00C043 Smoked Bacon II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es a
monocytogen Adri
48 Listeria A00C044 Poultry (duck) II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es a
monocytogen RTE food (Osso bucco with Adri
49 Listeria A00C052 II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es turkey) a
monocytogen IV Adri
50 Listeria A00C054 Beef heart + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen IV Adri
51 Listeria A00M032 Smoked salmon + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen Adri
52 Listeria A00M088 Smoked salmon II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es a
monocytogen Adri
es a
monocytogen Adri
es a
monocytogen Adri
es a
monocytogen Adri
es a
monocytogen Adri
es a
monocytogen Adri
es a
monocytogen Adri
es a
71/130
Alternative method
nce Final
Interpret result
compared
compared
Interpretati
(+/- (+/- (+/-
) ) )
monocytogen IV Adri
60 Listeria Ad268 Cured ham + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen IV Adri
61 Listeria Ad270 Fermented sausage + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen IV Adri
62 Listeria Ad272 Fermented sausage + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es b a
monocytogen Adri
es a
monocytogen Ready-to-eat food (Asiatic Adri
64 Listeria Ad274 II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
es meal) a
monocytogen Adri
es a
monocytogen Adri
es a
Adri
a
68 Listeria seeligeri Ad1267 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
sample a
Adri
69 Listeria seeligeri Ad1293 Parsley + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
70 Listeria seeligeri Ad1297 Merguez + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
71 Listeria seeligeri Ad1754 Zucchini + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
a
Adri
a
Adri
a
Adri
a
Adri
a
Adri
77 Listeria seeligeri Ad651 Environment sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
78 Listeria seeligeri Ad652 Dairy environmental sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
a
Adri
80 Listeria seeligeri BR18 Environment sample + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
72/130
Alternative method
nce Final
Interpret result
compared
compared
Interpretati
(+/- (+/- (+/-
) ) )
Adri
81 Listeria welshimeri Ad1175 Fried rize + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
82 Listeria welshimeri Ad1204 Ham + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
83 Listeria welshimeri Ad1211 Frozen ground beef meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
84 Listeria welshimeri Ad1223 Beef meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
85 Listeria welshimeri Ad1228 Turkey meat + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
86 Listeria welshimeri Ad1234 Sausages + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Slaughterhouse Adri
environment sample a
sample a
Adri
89 Listeria welshimeri Ad1667 Raw milk cheese + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
90 Listeria welshimeri Ad1670 Rosette + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Adri
91 Listeria welshimeri Ad1671 Smoked sausage + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
a
Q
ATCC NO No No NO No No NO No No
92 Listeria coloradensis Not Available Lab + + PA IA + PA IA NOIP No result No result
BAA-2414 IP result result IP result result IP result result
s
Q
FSL F6- - (no NA (no NA (no
93 Listeria cornellensis Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA
s
Q
FSL S10- NO No No NO No No NO No No NOI
94 Listeria fleischmannii Not Available Lab + NOIP No result No result No result No result NOIP No result No result
1203 IP result result IP result result IP result result P
s
Q
FSL S10- NO No No NO No No NO No No NOI
95 Listeria floridensis Not Available Lab + NOIP No result No result No result No result NOIP No result No result
1187 IP result result IP result result IP result result P
s
Q
FSL F6- NO No No
96 Listeria grandensis Not Available Lab + + PA IA + PA IA No peak No result No result + PA IA NOIP No result No result
0971 IP result result
s
Q
ATCC - (no NA (no NA (no
s
Q
NCTC - (no NA (no NA (no
s
73/130
Alternative method
nce Final
Interpret result
compared
compared
Interpretati
(+/- (+/- (+/-
) ) )
Q
QL - (no NA (no NA (no
99 Listeria grayi Industry Lab + + PA IA + PA IA + PA IA + PA IA + PA IA
030911-12 growth) growth) growth)
s
Q
s
Q
s
Q
s
Q
s
Q
s
Q
s
Q
s
Q
10 UVM - (no NA (no NA (no - (no NA (no NA (no
Listeria grayi Not Available Lab + + PA IA + PA IA + PA IA + PA IA
7 LGRA001 growth) growth) growth) growth) growth) growth)
s
Q
10 ATCC
Listeria innocua Cow brain Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
8 33090
s
Q
10 QL
9 030911-1
s
Q
11 QL
0 030911-2
s
Q
11 QL
1 32811.1
s
Q
11 QL
2 32811.2
s
74/130
Alternative method
nce Final
Interpret result
compared
compared
Interpretati
(+/- (+/- (+/-
) ) )
Q
11 QL
3 32811.3
s
Q
11
Listeria innocua UVM 11/2 Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
4
s
Q
11
5
s
Q
11
6
s
Q
11
7
s
Q
11
8
s
Q
11
9
s
Q
12
0
s
Q
12
1
s
Q
12
2
s
Q
12
3
s
Q
12 ATCC
Listeria ivanovii Washing water Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
4 BAA-139
s
Q
12 ATCC
Listeria ivanovii Sheep fetus Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
5 BAA-678
s
Q
12 QL
Listeria ivanovii Clinical Isolate Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
6 030911-10
s
75/130
Alternative method
nce Final
Interpret result
compared
compared
Interpretati
(+/- (+/- (+/-
) ) )
Q
12 QL
Listeria ivanovii Clinical Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
7 030911-11
s
Q
12 UVM
8 Liv004
s
Q
12 UVM
9 Liv005
s
Q
13 UVM
0 Liv006
s
Q
13 QL
Listeria londoniensis Clinical Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
1 030911-9
s
Q
13 ATCC
Listeria marthii Soil Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
2 BAA-1595
s
Q
13 FSL S10- NO No No NO No No NO No No NOI
Listeria riparia Not Available Lab + NOIP No result No result No result No result NOIP No result No result
3 1204 IP result result IP result result IP result result P
s
Q
13 FSL F6- NO No No NO No No NO No No NOI
Listeria rocourtiae Not Available Lab + NOIP No result No result No result No result NOIP No result No result
4 0920 IP result result IP result result IP result result P
s
Q
13 ATCC
Listeria seeligeri Human feces Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
5 11289
s
Q
s
Q
s
Q
13 ATCC Intesti'/l content of
Listeria seeligeri Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
8 51334 Clethrionomys glareolus
s
Q
13 ATCC
9 51335
s
Q
14 FSL-S4-
0 035
s
76/130
Alternative method
nce Final
Interpret result
compared
compared
Interpretati
(+/- (+/- (+/-
) ) )
Q
14 QL
1 031011-1
s
Q
14 QL
2 031011-2
s
Q
14 QL
3 031011-5
s
Q
14 QL
4 030911-6
s
Q
14 weihensteph FSL R9- - (no NA (no NA (no
Listeria Not Available Lab + + PA IA + PA IA + PA IA + PA IA + PA IA
5 enensis 0317 growth) growth) growth)
s
Q
14 ATCC
6 43548
s
Q
14 ATCC
Listeria welshimeri Soil Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
7 43549
s
Q
14 ATCC
Listeria welshimeri Human feces Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
8 43550
s
Q
14 ATCC
9 43551
s
Q
15 QL
0 030911-7
s
Q
15 QL
1 030911-8
s
Q
15 QL
Listeria welshimeri Unknown Lab + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
2 040713.1
s
Q
15 UVM
3 LW001
s
Q
15 UVM
4 LW002
s
77/130
Alternative method
nce Final
Interpret result
compared
compared
Interpretati
(+/- (+/- (+/-
) ) )
Q
15 UVM
5 LW003
s
N 155 155 155 155 155 155
PA 150 150 145 149 147 134
ND 0 0 0 0 0 0
Tot NA 0 0 0 0 0 0
al NA (no
0 0 5 0 4 15
growth)
PD 0 0 0 0 0 0
No result 5 5 5 6 4 6
78/130
Annex H - Results exclusivity study- Listeria spp.
NOIP: NO Organism Identification Possible

EXCLUSIVITY - Listeria spp
Alternative method
Non-target Strain
LT/SH MALDI-MS System
rDNA TSYEA O&A Palcam Oxford MOX RAPID'L.mono
sequencing Final Altern Final Final Final Final Final
ative Alternati Alternati Alternati Alternati Alternati
Refer resul metho result ve result ve result ve result ve result ve
ence t d method method method method method
Referen meth confir confirmat confirmat confirmat confirmat confirmat
N Species Sub species Origin Lab Final Final Final Final Final Final
od mation ion ion ion ion ion
ce Interpr Interpret Interpret Interpret Interpret Interpret
compa compare compare compare compare compare
etation ation ation ation ation ation
red to d to d to d to d to d to
(+/-) identit (+/-) identity (+/-) identity (+/-) identity (+/-) identity (+/-) identity
y of of the of the of the of the of the
the strain strain strain strain strain
strain
- (no - (no - (no
Ready to eat Adri NA (no NA (no NA (no NA (no NA (no NA (no
1 Bacillus cereus 16 - - NA EA growth - NA EA - NA EA growth growth
(seafood) a growth) growth) growth) growth) growth) growth)
) ) )
- (no - (no - (no - (no - (no
Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
2 Bacillus cereus 21 Cooked rice - - NA EA growth growth growth growth growth
a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no
Adri NA (no NA (no
3 Bacillus circulans Ad734 Dairy product - - NA EA - NA EA growth - NA EA - NA EA - NA EA
a growth) growth)
)
- (no - (no - (no - (no - (no
4 Bacillus circulans Ad760 Mashed vegetables - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
5 Bacillus coagulans Ad732 Dairy product - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
6 Bacillus coagulans Ad1281 Raw milk - - NA EA growth growth growth growth growth
) ) ) ) )
- (no
licheniformi Adri NA (no NA (no
7 Bacillus Ad741 Dairy product - - NA EA - NA EA growth - NA EA - NA EA - NA EA
s a growth) growth)
)
licheniformi Adri
8 Bacillus Ad798 Egg product - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
s a
- (no - (no - (no - (no - (no
9 Bacillus mycoïdes Ad763 Pasteurized vegetable - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
pseudomyc Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
10 Bacillus Ad767 Dairy product - - NA EA growth growth growth growth growth
oides a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
11 Bacillus Ad765 Vegetables - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no
Adri NA (no NA (no NA (no NA (no
12 Bacillus pumilus Ad733 Dairy product - - NA EA - NA EA growth - NA EA growth - NA EA
a growth) growth) growth) growth)
) )
- (no
Adri NA (no NA (no
13 Bacillus pumilus Ad768 Vegetables - - NA EA - NA EA - NA EA - NA EA growth - NA EA
a growth) growth)
)
79/130
Alternative method
Non-target Strain
strain
- (no - (no - (no - (no - (no
14 Bacillus subtilis Ad839 Chocolate - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
thuringiensi Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
s a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
16 Bacillus Ad2067 Uknow - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
weihenstep Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
17 Bacillus Ad726 Egg product - - NA EA growth growth growth growth growth
hanensis a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
18 Bacillus Ad778 Purée - - NA EA growth growth growth growth growth
) ) ) ) )
Enterococcus EA - (no - (no - (no - (no - (no
Enterococc Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
19 avium Ad 183 Whole egg - - NA growth growth growth growth growth
us malodoratus a EA growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
Enterococc casseliflavu Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
20 Ad1346 Water - - NA EA growth growth growth growth growth
us s a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
Enterococcus - (no - (no - (no - (no - (no
21 durans Ad 181 faecium / hirae Whole egg - - NA EA growth growth growth growth growth
us a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
/ durans ) ) ) ) )
- (no - (no - (no
Enterococc Adri NA (no NA (no NA (no NA (no NA (no NA (no
22 faecalis Ad 602 Raw milk - - NA EA growth growth - NA EA - NA EA growth
us a growth) growth) growth) growth) growth) growth)
) ) )
- (no
Enterococc Adri NA (no NA (no
23 faecalis 25 Poultry meat - - NA EA - NA EA - NA EA - NA EA - NA EA growth
us a growth) growth)
)
- (no - (no
Enterococc Adri NA (no NA (no NA (no NA (no
24 faecalis Ad547 Pancake - - NA EA growth - NA EA - NA EA growth - NA EA
us a growth) growth) growth) growth)
) )
- (no - (no - (no - (no - (no
Enterococc Pasteurised whole Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
25 faecium Ad177 - - NA EA growth growth growth growth growth
us egg a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
26 gallinarum Ad1145 casseliflavus / Guacamole - - NA EA growth growth growth growth growth
gallinarum ) ) ) ) )
- (no - (no - (no - (no
Enterococc Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
27 hirae Ad1392 Sea water - - NA EA growth growth - NA EA growth growth
us a growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) )
80/130
Alternative method
Non-target Strain
strain
- (no
28 mundtii Ad1365 Stream - - NA EA - NA EA - NA EA - NA EA - NA EA growth
)
- (no - (no - (no - (no - (no
Lactobacill Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
29 acidophilus 93L822 Raw milk - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
30 sakei Ad1150 Delicatessen - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
31 sunkii Ad1819 Ready to eat - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
Lactococcu Leuconostoc Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
32 casei Ad469 Whole egg - - NA EA growth growth growth growth growth
s mesenteroides a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
Lactococcu Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
33 lactis Ad 425 Ferment - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
34 plantarum Ad1147 Guacamole - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
Leuconost Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
35 carnosus Ad386 Ham - - NA EA growth growth growth growth growth
oc a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
Leuconost Enterococcus Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
36 citreum Ad604 Raw milk - - NA EA growth growth growth growth growth
oc faecalis a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
Leuconost pseudomes Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
37 Ad835 Dairy desert - - NA EA growth growth growth growth growth
oc enteroïdes a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no
Lysinibacill Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
38 sphaericus Ad872 Dairy product - - NA EA growth growth - NA EA growth growth
) ) ) )
- (no - (no
Lysinibacill Lysinibacillus Adri NA (no NA (no NA (no NA (no No No No
39 sphaericus Ad724 Unknown - - NA EA growth growth - NA EA - NA EA
us fusiformis a growth) growth) growth) growth) peak result result
) )
- (no - (no - (no - (no - (no
Pseudomo Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
40 fragi Ad1327 Whole liquid egg - - NA EA growth growth growth growth growth
nas a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
41 veronii Ad1588 Environmental sample - - NA EA growth growth growth growth growth
) ) ) ) )
81/130
Alternative method
Non-target Strain
strain
- (no - (no - (no
Staphyloco Adri NA (no NA (no No No NA (no NA (no NA (no NA (no
42 aureus Ad906 Sausage - - NA EA growth - NA EA NOIP growth growth
ccus a growth) growth) result result growth) growth) growth) growth)
) ) )
- (no - (no - (no - (no - (no
Staphyloco Micrococcus Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
43 epidermidis Ad931 Fruit - - NA EA growth growth growth growth growth
ccus luteus a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no
Staphyloco Adri NA (no NA (no NA (no NA (no
44 equorum Ad1099 Cheese - - NA EA growth - NA EA - NA EA growth - NA EA
ccus a growth) growth) growth) growth)
) )
- (no
Staphyloco haemolyticu Adri NA (no NA (no
45 Ad989 Dairy product - - NA EA - NA EA - NA EA - NA EA growth - NA EA
ccus s a growth) growth)
)
- (no - (no - (no - (no
Staphyloco Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
46 hominis Ad849 Unknow - - NA EA growth growth - NA EA growth growth
ccus a growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) )
- (no - (no - (no
Staphyloco CIP81.5 Adri NA (no NA (no NA (no NA (no NA (no NA (no
47 hyicus Porc - - NA EA growth - NA EA - NA EA growth growth
ccus 8 a growth) growth) growth) growth) growth) growth)
) ) )
Staphylococcu
s intermedius / - (no - (no - (no - (no - (no
Staphyloco Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
48 intermedius CIP8177 delphini / Dog - - NA EA growth growth growth growth growth
ccus a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
pseudintermedi ) ) ) ) )
us
- (no - (no
49 pasteurii Ad1717 Whole egg - - NA EA growth - NA EA - NA EA growth - NA EA
) )
- (no - (no - (no
Staphyloco saprophytic Adri NA (no NA (no NA (no NA (no NA (no NA (no
50 Ad866 Milk - - NA EA growth - NA EA - NA EA growth growth
ccus us a growth) growth) growth) growth) growth) growth)
) ) )
- (no - (no - (no - (no - (no
Streptococ Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
51 bovis 92L613 Cheese - - NA EA growth growth growth growth growth
cus a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
52 salivarius Ad 441 Milk - - NA EA growth growth growth growth growth
) ) ) ) )
Q - (no - (no - (no - (no - (no
Aeromona ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
53 hydrophila Clinical Isolate Lab - - NA EA growth growth growth growth growth
s 49140 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Aerococcu QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
54 viridans Raw Milk Lab - - NA EA growth growth growth growth growth
s 17041.8 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
82/130
Alternative method
Non-target Strain
strain
Q - (no - (no - (no - (no - (no
QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
55 Bacillus cereus Psyllium Lab - - NA EA growth growth growth growth growth
15166-1 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
licheniformi ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
56 Bacillus Cassava tuber plant Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Burkholderi ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
57 cepacia Plant-derived foodstuff Lab - - NA EA growth growth growth growth growth
a* 25416 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q No - (no - (no - (no - (no - (no
Carnobact ATCC Ice slush from around No No NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
58 gallinarum Lab - pea growth growth growth growth growth
erium 49517 chicken carcasses result result growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s k ) ) ) ) )
Q - (no - (no - (no - (no - (no
Carnobact maltaromati ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
59 Vacuum-packed beef Lab - - NA EA growth growth growth growth growth
erium cum 43224 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
amolanaticu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
60 Citrobacter Feces Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
61 Citrobacter farmeri Human Feces Lab - - NA EA growth growth growth growth growth
51633 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Cronobact NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
62 malonaticus 123015- Rice flour Lab - - NA EA growth growth growth growth growth
er growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
1A s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Cryptococc ATCC Clinical specimen. NOI No No NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
63 albidus Lab - growth growth growth growth growth
us 34140 Minnesota P result result growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Edwardsiel NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
64 tarda 11007- Clinical Isolate Lab - - NA EA growth growth growth growth growth
la growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
11 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Enterobact ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
65 aerogenes Not Available Lab - - NA EA growth growth growth growth growth
er 35029 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
66 amnigenus Milk Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Shigella, Q - (no - (no - (no - (no - (no
Enterobact cancerogen QL1101 NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
67 Escherichia, Bottled Water Lab - - NA EA growth growth growth growth growth
er us 0.1 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
Enterobacter s ) ) ) ) )
Q - (no - (no - (no - (no - (no
68 cloacae Spinal Fluid Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
83/130
Alternative method
Non-target Strain
strain
Q - (no - (no - (no - (no - (no
Enterococc ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
69 faecium Dairy Product Lab - - NA EA growth growth growth growth growth
us 8459 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
70 faecalis Urine Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Escherichi QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
71 coli Feces Lab - - NA EA growth growth growth growth growth
a 11010.2 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
ATCC Clinical isolate. NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
72 Klebsiella oxytoca Lab - - NA EA growth growth growth growth growth
43165 Califonria growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
73 Klebsiella pneumoniae Not Available Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
74 Kocuria rhizophila Soil Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q No - (no - (no - (no - (no - (no
ATCC No No NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
75 Kurthia gobsonii Meat Lab - pea growth growth growth growth growth
43195 result result growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s k ) ) ) ) )
Q No - (no - (no - (no - (no - (no
76 Kurthia zopfii Not Available Lab - pea growth growth growth growth growth
s k ) ) ) ) )
Q - (no - (no - (no - (no - (no
Lactobacill ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
77 casei Oral cavity Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
78 cremoris Not Available Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Leuconost mesenteroid NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
79 071011- Sauerkraut Lab - - NA EA growth growth growth growth growth
oc es growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
7 s ) ) ) ) )
ATCC Q - (no
Lysinibacill NA (no NA (no
80 sphaericus BAA- / Not Available Lab - - NA EA - NA EA growth - NA EA - NA EA - NA EA
us growth) growth)
378 s )
Q - (no - (no - (no - (no - (no
Pseudomo extremorien QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
81 Raw milk Lab - - NA EA growth growth growth growth growth
nas tialis 17041.1 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Pseudomo NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
82 gessardii 17041.1 Raw milk Lab - - NA EA growth growth growth growth growth
nas growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
2 s ) ) ) ) )
84/130
Alternative method
Non-target Strain
strain
Q - (no - (no - (no - (no - (no
Pseudomo QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
83 proteolytica Raw milk Lab - - NA EA growth growth growth growth growth
nas 17041.4 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Pseudomo ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
84 aeruginosa Not Available Lab - - NA EA growth growth growth growth growth
nas 35032 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
85 fluorescens Raw milk Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
86 rhodesiae Raw milk Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
87 Proteus mirabilis Veterinary Lab - - NA EA growth growth growth growth growth
11007.6 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Providenci QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
88 stuartii Clinical Isolate Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Rhodococc ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
89 equi Lung abscess of foal Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Rhodococc NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
90 fascians 052712- Fruit Salad Lab - - NA EA growth growth growth growth growth
us growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
3 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
91 Salmonella Galiema QL024.2 Environmental Isolate Lab - - NA EA growth growth growth growth growth
growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Typhimuriu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
92 Salmonella Animal tissue Lab - - NA EA growth growth growth growth growth
m 14028 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Staphyloco NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
93 aureus 030911. Seasoning Powder Lab - - NA EA growth growth growth growth growth
ccus growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
4 s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Staphyloco chromogen NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
94 17041.1 Raw milk Lab - - NA EA growth growth growth growth growth
ccus es growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
0 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Staphyloco ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
95 epidermidis Not Available Lab - - NA EA growth growth growth growth growth
ccus 12228 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no
Staphyloco haemolyticu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
96 Human skin Lab - - NA EA growth growth - NA EA growth growth
ccus s 29970 growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) )
85/130
Alternative method
Non-target Strain
strain
QL Q - (no - (no - (no - (no - (no
97 hyicus 030911. Seasoning Powder Lab - - NA EA growth growth growth growth growth
3 s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
98 intermedius 030911. Veterinary Lab - - NA EA growth growth growth growth growth
2 s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Staphyloco saprophytic NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
99 090110- Pasteurized Milk Lab - - NA EA growth growth growth growth growth
ccus us growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
8 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
10 Staphyloco QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
sciuri Raw milk Lab - - NA EA growth growth growth growth growth
0 ccus 17041.9 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no
10 Staphyloco ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
warneri Not Available Lab - - NA EA growth growth - NA EA growth growth
1 ccus 29885 growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) )
Q - (no - (no - (no - (no - (no
10 Streptococ ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
pneumoniae Not Available Lab - - NA EA growth growth growth growth growth
2 cus 6302 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
10 Streptococ NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
salivarius 011509- Pasteurized Milk Lab - - NA EA growth growth growth growth growth
3 cus growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
7 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
10 parahaemol CCUG NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
Vibrio Sea Food Lab - - NA EA growth growth growth growth growth
4 yticus 43364 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
10 NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
Vibrio vulnificus 021111 Seafood Product Lab - - NA EA growth growth growth growth growth
B s ) ) ) ) )
Q - (no - (no - (no - (no - (no
10 ATCC Clinical Isolate. NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
Yersinia enterolytica Lab - - NA EA growth growth growth growth growth
6 49397 Human growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q No - (no - (no - (no - (no - (no
10 Zygosacch ATCC No No NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
bailii Not Available Lab - pea growth growth growth growth growth
7 aromyces 36947 result result growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s k ) ) ) ) )
N 107 107 107 107 107 107
PA 0 0 0 0 0 0
ND 0 0 0 0 0 0
To NA 102 9 12 21 8 11
tal NA (no
0 98 95 84 99 96
growth)
PD 0 0 0 0 0 0
No result 5 0 0 2 0 0
86/130
EXCLUSIVITY - Listeria spp.
Alternative method
Non-target Strain
LT/SH MALDI-smart System
Altern Final Final
Final Final
ative Alternati Alternati result Alternati result Alternati Alternati
Refer metho result ve result ve (+/-) ve (+/-) ve ve
ence d method method method method method
Referen rDNA meth Final confir confirmat confirmat confirmat confirmat confirmat
N° Species Sub species Origin Lab Final Final Final Final Final Final Final
od resul mation ion ion ion ion ion
ce sequencing Interpr Interpret Interpret Interpret Interpret result Interpret
t (+/- compa compare compare compare compare compare
etation ation ation ation ation (+/-) ation
) red to (+/-) d to (+/-) d to d to d to d to
identit identity identity identity identity identity
strain
- (no - (no - (no
) ) )
- (no - (no - (no - (no - (no
2 Bacillus cereus 21 Cooked rice - - NA EA growth growth growth growth growth
) ) ) ) )
- (no
Adri NA (no NA (no
3 Bacillus circulans Ad734 Dairy product - - NA EA - NA EA growth - NA EA - NA EA - NA EA
a growth) growth)
)
- (no - (no - (no - (no - (no
4 Bacillus circulans Ad760 Mashed vegetables - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
Adri NOI No No NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
5 Bacillus coagulans Ad732 Dairy product - growth growth growth growth growth
a P result result growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
Adri NOI No No NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
6 Bacillus coagulans Ad1281 Raw milk - growth growth growth growth growth
a P result result growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no
7 Bacillus Ad741 Dairy product - - NA EA - NA EA growth - NA EA - NA EA - NA EA
s a growth) growth)
)
licheniformi Adri
8 Bacillus Ad798 Egg product - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
s a
- (no - (no - (no - (no - (no
9 Bacillus mycoïdes Ad763 Pasteurized vegetable - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
10 Bacillus Ad767 Dairy product - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
) ) ) ) )
- (no - (no
12 Bacillus pumilus Ad733 Dairy product - - NA EA - NA EA growth - NA EA growth - NA EA
) )
- (no
Adri NA (no NA (no
13 Bacillus pumilus Ad768 Vegetables - - NA EA - NA EA - NA EA - NA EA growth - NA EA
a growth) growth)
)
- (no - (no - (no - (no - (no
14 Bacillus subtilis Ad839 Chocolate - - NA EA growth growth growth growth growth
) ) ) ) )
87/130
Alternative method
Non-target Strain
Altern Final Final
Final Final
strain
- (no - (no - (no - (no - (no
) ) ) ) )
- (no - (no - (no - (no - (no
16 Bacillus Ad2067 Uknow - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
17 Bacillus Ad726 Egg product - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
18 Bacillus Ad778 Purée - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
Enterococc Enterococcus Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
19 avium Ad 183 Whole egg - - NA EA growth growth growth growth growth
us malodoratus a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
Enterococc casseliflavu Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
20 Ad1346 Water - - NA EA growth growth growth growth growth
us s a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
21 durans Ad 181 faecium / hirae Whole egg - - NA EA growth growth growth growth growth
/ durans ) ) ) ) )
- (no - (no - (no
Enterococc Adri NA (no NA (no NA (no NA (no NA (no NA (no
22 faecalis Ad 602 Raw milk - - NA EA growth growth - NA EA - NA EA growth
us a growth) growth) growth) growth) growth) growth)
) ) )
- (no
23 faecalis 25 Poultry meat - - NA EA - NA EA - NA EA - NA EA - NA EA growth
)
- (no - (no
Enterococc Adri NA (no NA (no NA (no NA (no
24 faecalis Ad547 Pancake - - NA EA growth - NA EA - NA EA growth - NA EA
us a growth) growth) growth) growth)
) )
- (no - (no - (no - (no - (no
Enterococc Pasteurised whole Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
25 faecium Ad177 - - NA EA growth growth growth growth growth
us egg a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
26 gallinarum Ad1145 casseliflavus / Guacamole - - NA EA growth growth growth growth growth
gallinarum ) ) ) ) )
- (no - (no - (no - (no
Enterococc Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
27 hirae Ad1392 Sea water - - NA EA growth growth - NA EA growth growth
) ) ) )
- (no
28 mundtii Ad1365 Stream - - NA EA - NA EA - NA EA - NA EA - NA EA growth
)
88/130
Alternative method
Non-target Strain
Altern Final Final
Final Final
strain
- (no - (no - (no - (no - (no
29 acidophilus 93L822 Raw milk - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
30 sakei Ad1150 Delicatessen - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
31 sunkii Ad1819 Ready to eat - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
Lactococcu Leuconostoc Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
32 casei Ad469 Whole egg - - NA EA growth growth growth growth growth
s mesenteroides a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
33 lactis Ad 425 Ferment - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
34 plantarum Ad1147 Guacamole - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
Leuconost Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
35 carnosus Ad386 Ham - - NA EA growth growth growth growth growth
oc a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
Leuconost Enterococcus Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
36 citreum Ad604 Raw milk - - NA EA growth growth growth growth growth
oc faecalis a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no - (no
Leuconost pseudomes Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
37 Ad835 Dairy desert - - NA EA growth growth growth growth growth
oc enteroïdes a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no - (no - (no
Lysinibacill Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
38 sphaericus Ad872 Dairy product - - NA EA growth growth - NA EA growth growth
) ) ) )
- (no - (no No
Lysinibacill Lysinibacillus Adri NA (no NA (no NA (no NA (no No No No No
39 sphaericus Ad724 Unknown - - NA EA growth growth - NA EA peak NOIP
us fusiformis a growth) growth) growth) growth) result result result result
) ) found
- (no - (no - (no - (no - (no
40 fragi Ad1327 Whole liquid egg - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
41 veronii Ad1588 Environmental sample - - NA EA growth growth growth growth growth
) ) ) ) )
- (no No - (no - (no
Staphyloco Adri NA (no NA (no No No NA (no NA (no NA (no NA (no
42 aureus Ad906 Sausage - - NA EA growth - NA EA peak growth growth
ccus a growth) growth) result result growth) growth) growth) growth)
) found ) )
89/130
Alternative method
Non-target Strain
Altern Final Final
Final Final
strain
- (no - (no - (no - (no - (no
Staphyloco Micrococcus Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
43 epidermidis Ad931 Fruit - - NA EA growth growth growth growth growth
ccus luteus a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) ) ) ) )
- (no - (no
) )
- (no
Staphyloco haemolyticu Adri NA (no NA (no
ccus s a growth) growth)
)
- (no - (no - (no - (no
Staphyloco Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
46 hominis Ad849 Unknow - - NA EA growth growth - NA EA growth growth
) ) ) )
- (no - (no - (no
Staphyloco CIP81.5 Adri NA (no NA (no NA (no NA (no NA (no NA (no
ccus 8 a growth) growth) growth) growth) growth) growth)
) ) )
Staphylococcu
s intermedius / - (no - (no - (no - (no - (no
Staphyloco Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
48 intermedius CIP8177 delphini / Dog - - NA EA growth growth growth growth growth
pseudintermedi ) ) ) ) )
us
- (no - (no
49 pasteurii Ad1717 Whole egg - - NA EA growth - NA EA - NA EA growth - NA EA
) )
- (no - (no - (no
Staphyloco saprophytic Adri NA (no NA (no NA (no NA (no NA (no NA (no
50 Ad866 Milk - - NA EA growth - NA EA - NA EA growth growth
ccus us a growth) growth) growth) growth) growth) growth)
) ) )
- (no - (no - (no - (no - (no
51 bovis 92L613 Cheese - - NA EA growth growth growth growth growth
) ) ) ) )
- (no - (no - (no - (no - (no
52 salivarius Ad 441 Milk - - NA EA growth growth growth growth growth
) ) ) ) )
Q - (no - (no - (no - (no - (no
Aeromona ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
53 hydrophila Clinical Isolate Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Aerococcu QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
54 viridans Raw Milk Lab - - NA EA growth growth growth growth growth
s 17041.8 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
55 Bacillus cereus Psyllium Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
90/130
Alternative method
Non-target Strain
Altern Final Final
Final Final
strain
Q - (no - (no - (no - (no - (no
licheniformi ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
56 Bacillus Cassava tuber plant Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Burkholderi ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
57 cepacia Plant-derived foodstuff Lab - - NA EA growth growth growth growth growth
a* 25416 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q No - (no - (no - (no - (no - (no
Carnobact ATCC Ice slush from around No No NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
58 gallinarum Lab - pea growth growth growth growth growth
erium 49517 chicken carcasses result result growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s k ) ) ) ) )
Q - (no - (no - (no - (no - (no
Carnobact maltaromati ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
59 Vacuum-packed beef Lab - - NA EA growth growth growth growth growth
erium cum 43224 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
amolanaticu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
60 Citrobacter Feces Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
61 Citrobacter farmeri Human Feces Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Cronobact NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
62 malonaticus 123015- Rice flour Lab - - NA EA growth growth growth growth growth
er growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
1A s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Cryptococc ATCC Clinical specimen. NOI No No NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
63 albidus Lab - growth growth growth growth growth
us 34140 Minnesota P result result growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Edwardsiel NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
64 tarda 11007- Clinical Isolate Lab - - NA EA growth growth growth growth growth
la growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
11 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
65 aerogenes Not Available Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
66 amnigenus Milk Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Shigella, Q - (no - (no - (no - (no - (no
Enterobact cancerogen QL1101 NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
67 Escherichia, Bottled Water Lab - - NA EA growth growth growth growth growth
er us 0.1 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
Enterobacter s ) ) ) ) )
Q - (no - (no - (no - (no - (no
68 cloacae Spinal Fluid Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
69 faecium Dairy Product Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
91/130
Alternative method
Non-target Strain
Altern Final Final
Final Final
strain
Q - (no - (no - (no - (no - (no
70 faecalis Urine Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Escherichi QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
71 coli Feces Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
ATCC Clinical isolate. NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
72 Klebsiella oxytoca Lab - - NA EA growth growth growth growth growth
43165 Califonria growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
73 Klebsiella pneumoniae Not Available Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
74 Kocuria rhizophila Soil Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q No - (no - (no - (no - (no - (no
75 Kurthia gobsonii Meat Lab - pea growth growth growth growth growth
s k ) ) ) ) )
Q No - (no - (no - (no - (no - (no
76 Kurthia zopfii Not Available Lab - pea growth growth growth growth growth
s k ) ) ) ) )
Q - (no - (no - (no - (no - (no
77 casei Oral cavity Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
78 cremoris Not Available Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Leuconost mesenteroid NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
79 071011- Sauerkraut Lab - - NA EA growth growth growth growth growth
oc es growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
7 s ) ) ) ) )
ATCC Q - (no
Lysinibacill NA (no NA (no
80 sphaericus BAA- / Not Available Lab - - NA EA - NA EA growth - NA EA - NA EA - NA EA
us growth) growth)
378 s )
Q - (no - (no - (no - (no - (no
Pseudomo extremorien QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
81 Raw milk Lab - - NA EA growth growth growth growth growth
nas tialis 17041.1 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Pseudomo NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
82 gessardii 17041.1 Raw milk Lab - - NA EA growth growth growth growth growth
nas growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
2 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
83 proteolytica Raw milk Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
92/130
Alternative method
Non-target Strain
Altern Final Final
Final Final
strain
Q - (no - (no - (no - (no - (no
Pseudomo ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
84 aeruginosa Not Available Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
85 fluorescens Raw milk Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
86 rhodesiae Raw milk Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
87 Proteus mirabilis Veterinary Lab - - NA EA growth growth growth growth growth
11007.6 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Providenci QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
88 stuartii Clinical Isolate Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Rhodococc ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
89 equi Lung abscess of foal Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Rhodococc NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
90 fascians 052712- Fruit Salad Lab - - NA EA growth growth growth growth growth
us growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
3 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
91 Salmonella Galiema QL024.2 Environmental Isolate Lab - - NA EA growth growth growth growth growth
growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Typhimuriu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
92 Salmonella Animal tissue Lab - - NA EA growth growth growth growth growth
m 14028 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
93 aureus 030911. Seasoning Powder Lab - - NA EA growth growth growth growth growth
4 s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Staphyloco chromogen NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
94 17041.1 Raw milk Lab - - NA EA growth growth growth growth growth
ccus es growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
0 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Staphyloco ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
95 epidermidis Not Available Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q - (no - (no - (no - (no
Staphyloco haemolyticu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
96 Human skin Lab - - NA EA growth growth - NA EA growth growth
ccus s 29970 growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) )
QL Q - (no - (no - (no - (no - (no
97 hyicus 030911. Seasoning Powder Lab - - NA EA growth growth growth growth growth
3 s ) ) ) ) )
93/130
Alternative method
Non-target Strain
Altern Final Final
Final Final
strain
QL Q - (no - (no - (no - (no - (no
98 intermedius 030911. Veterinary Lab - - NA EA growth growth growth growth growth
2 s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
Staphyloco saprophytic NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
99 090110- Pasteurized Milk Lab - - NA EA growth growth growth growth growth
ccus us growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
8 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
10 Staphyloco QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
sciuri Raw milk Lab - - NA EA growth growth growth growth growth
0 ccus 17041.9 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
Q - (no - (no - (no - (no
10 Staphyloco ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
warneri Not Available Lab - - NA EA growth growth - NA EA growth growth
1 ccus 29885 growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) )
Q - (no - (no - (no - (no - (no
10 Streptococ ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
pneumoniae Not Available Lab - - NA EA growth growth growth growth growth
2 cus 6302 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
10 Streptococ NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
salivarius 011509- Pasteurized Milk Lab - - NA EA growth growth growth growth growth
3 cus growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
7 s ) ) ) ) )
Q - (no - (no - (no - (no - (no
10 parahaemol CCUG NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
Vibrio Sea Food Lab - - NA EA growth growth growth growth growth
4 yticus 43364 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) ) ) ) )
QL Q - (no - (no - (no - (no - (no
10 NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
Vibrio vulnificus 021111 Seafood Product Lab - - NA EA growth growth growth growth growth
B s ) ) ) ) )
Q - (no - (no - (no - (no - (no
Yersinia enterolytica Lab - - NA EA growth growth growth growth growth
s ) ) ) ) )
Q No - (no - (no - (no - (no - (no
10 Zygosacch ATCC No No NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
bailii Not Available Lab - pea growth growth growth growth growth
7 aromyces 36947 result result growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s k ) ) ) ) )
N 107 107 107 107 107 107
PA 0 0 0 0 0 0
ND 0 0 0 0 0 0
To NA 100 9 12 22 7 10
tal NA (no
0 98 95 84 99 96
growth)
PD 0 0 0 0 0 0
No result 7 0 0 1 1 1
94/130
Annex I - Results inclusivity study - Listeria monocytogenes
INCLUSIVITY - Listeria monocytogenes

Alternative method
Alternati Alternati Alternati Alternati Alternati Alternati
Refere ve ve ve ve ve ve
nce Fin method Fin method Fin method Fin method Fin method Fin method
metho al confirma al confirma al confirma al confirma al confirma al confirma
Final Final Final Final Final Final
Referen Seroty d res tion res tion res tion res tion res tion res tion
N Genus Species Origin Lab Interpret Interpret Interpret Interpret Interpret Interpret
ce pe ult compare ult compare ult compare ult compare ult compare ult compare
ation ation ation ation ation ation
(+/- d to (+/- d to (+/- d to (+/- d to (+/- d to (+/- d to
) identity ) identity ) identity ) identity ) identity ) identity
of the of the of the of the of the of the
strain strain strain strain strain strain
monocytog Adri
enes a
monocytog 1972/23 Adri
2 Listeria Puff pastry with mushrooms VI b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 99 a
monocytog 1973/24 Puff pastry egg and ham (Quiche Adri
3 Listeria VI b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 00 lorraine) a
4 Listeria Tripes with tomatoes IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 39 a
monocytog Adri
enes a
6 Listeria Smoked bacon IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 79 a
7 Listeria Pâté (Rillettes) IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 16 a
8 Listeria Black pudding IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 048 a
monocytog A00C04 Adri
9 Listeria Cooked delicatessen (Museau) IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 0 a
10 Listeria Sausage La + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 1 a
11 Listeria Raw sausage IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 2 a
12 Listeria Smoked Bacon II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 3 a
13 Listeria Poultry (duck) II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 4 a
monocytog A00C05 RTE food (Osso bucco with Adri
14 Listeria II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 2 turkey) a
15 Listeria Beef heart IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 4 a
monocytog A00M03 Adri
16 Listeria Smoked salmon IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 2 a
17 Listeria Smoked salmon II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 8 a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
95/130
Alternative method
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
29 Listeria Ad274 Ready-to-eat food (Asiatic meal) II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
32 Listeria Ad534 Fruits II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
33 Listeria Ad544 Onion II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
34 Listeria Ad546 Flour II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
35 Listeria Ad618 Soft cheese (Munster) IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
36 Listeria Ad623 Bread crumbs II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
37 Listeria Ad625 Dairy environmental sample IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
38 Listeria Ad626 Cheese (Gorgonzola) II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
39 Listeria Ad630 Cheese (Cantal) II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
40 Listeria Ad665 Milk II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog RTE food (deli salad with Adri
41 Listeria 850/109 II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes seafood) a
monocytog Adri
42 Listeria Ad1494 Strasbourg sausage II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
96/130
Alternative method
monocytog Adri
43 Listeria Ad1495 Deli salad II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
44 Listeria Ad1496 RT reheat (sea food) II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
45 Listeria Ad1497 Pasta II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
enes a
monocytog Adri
47 Listeria Ad1679 Fish environmental sample II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
48 Listeria Ad1781 Raw milk IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
49 Listeria Ad1784 Ewe milk II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
50 Listeria Ad816 Uknown La + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog ATCC Q
51 Listeria Human Isolate 1/2 C + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 7644 Labs
monocytog ATCC Spinal Fluid of child with Q
52 Listeria 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 13932 menintoitis. Germany Labs
QL
monocytog Q
53 Listeria 030911. Shellfish NA + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
3
QL
monocytog Q
enes Labs
4
QL
monocytog Q
enes Labs
5
monocytog ATCC Q
56 Listeria rabbit. Cambridge. England 1/2 A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 15313 Labs
monocytog ATCC Q
57 Listeria Poultry. England 1 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 19111 Labs
monocytog ATCC Q
58 Listeria Tissue. animal (ruminant brain) 4A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 19114 Labs
monocytog ATCC Q
59 Listeria Chicken. England 4C + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 19116 Labs
monocytog FSL Q
60 Listeria Not Available NA + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes J1.049 Labs
monocytog FSL J1- Q
enes 129 Labs
monocytog FSL F6- Q
enes 367 Labs
97/130
Alternative method
QL
monocytog Q
enes Labs
10
monocytog ATCC Q
64 Listeria Chicken. England 4E + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 19118 Labs
monocytog ATCC Q
65 Listeria Not Available N/A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 43256 Labs
monocytog ATCC Q
66 Listeria Not Available 1/2A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 49594 Labs
monocytog ATCC Q
enes 51772 Labs
monocytog ATCC Q
68 Listeria Diary Products (Belgium) 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 51778 Labs
monocytog ATCC51 Q
69 Listeria Dairy Products (Cheese) 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 780 Labs
monocytog ATCC51 Q
70 Listeria Dairy Products (Cheese) 3A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 782 Labs
ATCC
monocytog Q
71 Listeria BAA- Not Available 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
751
ATCC
monocytog Q
72 Listeria BAA Not Available N/A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
2658
monocytog Q
73 Listeria BEI Li2 Q Labs Isolate BEI NR-100 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
UVM
monocytog Q
74 Listeria CWD Not Available 1/2C + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1552
UVM
monocytog Q
75 Listeria CWD Carlisle 1981 1/2A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1554
UVM
monocytog Q
enes Labs
1555
UVM
monocytog Q
77 Listeria CWD Not Available 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1559
UVM
monocytog Q
enes Labs
1560
UVM
monocytog Q
79 Listeria CWD Mother/Baby. Placenta 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1561
98/130
Alternative method
UVM
monocytog Q
80 Listeria CWD Lausanne 1987 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1563
UVM
monocytog Q
81 Listeria CWD LA Outbreak 1985 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1567
UVM
monocytog Q
enes Labs
1571
UVM
monocytog Q
enes Labs
1599
UVM
monocytog Q
84 Listeria CWD Not Available 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1601
UVM
monocytog Q
85 Listeria CWD Turkey Frank Factory 1/2A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1609
UVM
monocytog Q
enes Labs
1612
UVM
monocytog Q
enes Labs
1613
UVM
monocytog Q
88 Listeria CWD Oklahoma 1/2A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1614
UVM
monocytog Q
enes Labs
1618
UVM
monocytog Q
enes Labs
1620
UVM
monocytog Q
91 Listeria CWD Oklahoma Turkey Franks 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1626
UVM
monocytog Q
92 Listeria CWD Mother/Baby 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1627
UVM
monocytog Q
93 Listeria CWD Oklahoma Turkey Franks 1/2A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1629
99/130
Alternative method
UVM
monocytog Q
enes Labs
1630
UVM
monocytog Q
95 Listeria CWD15 Unknown 1/2C + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
53
UVM
monocytog Q
96 Listeria CWD15 Halifax 1983 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
74
UVM
monocytog Q
97 Listeria CWD15 Not Available 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
84
UVM
monocytog Q
98 Listeria CWD15 Not Available 3B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
86
UVM
monocytog Q
enes Labs
88
UVM
10 monocytog Q
Listeria CWD15 LA 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
0 enes Labs
89
N 100 100 100 100 100 100
PA 100 100 100 100 100 100
ND 0 0 0 0 0 0
Tot
NA 0 0 0 0 0 0
al
NA (no
0 0 0 0 0 0
growth)
PD 0 0 0 0 0 0
100/130
Alternative method
Fin Alternati Fin Alternati Fin Alternati Fin Alternati Fin Alternati Fin Alternati
Refere al ve al ve al ve al ve al ve al ve
nce method method method method method method
confirma res confirma confirma confirma res confirma res confirma
metho res Final Final res Final res Final Final Final
Referen Serot tion ult tion tion tion ult tion ult tion
d ult ult ult
N Genus Species Origin Lab compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
ce ype tion tion tion tion tion tion
d to d to d to d to d to d to
(+/- identity (+/- identity identity (+/- identity (+/- identity identity
(+/-) (+/-)
) of the ) of the of the ) of the ) of the of the
monocytog Adri
enes a
2 Listeria Puff pastry with mushrooms VI b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 99 a
monocytog 1973/24 Puff pastry egg and ham (Quiche Adri
3 Listeria VI b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 00 lorraine) a
4 Listeria Tripes with tomatoes IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 39 a
monocytog Adri
enes a
6 Listeria Smoked bacon IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 79 a
7 Listeria Pâté (Rillettes) IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 16 a
8 Listeria Black pudding IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 048 a
9 Listeria Cooked delicatessen (Museau) IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 0 a
10 Listeria Sausage La + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 1 a
11 Listeria Raw sausage IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 2 a
12 Listeria Smoked Bacon II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 3 a
13 Listeria Poultry (duck) II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 4 a
monocytog A00C05 RTE food (Osso bucco with Adri
14 Listeria II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 2 turkey) a
15 Listeria Beef heart IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 4 a
16 Listeria Smoked salmon IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 2 a
17 Listeria Smoked salmon II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 8 a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
101/130
Alternative method
d ult ult ult
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
(+/-) (+/-)
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
29 Listeria Ad274 Ready-to-eat food (Asiatic meal) II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
enes a
monocytog Adri
enes a
monocytog Adri
32 Listeria Ad534 Fruits II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
33 Listeria Ad544 Onion II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
34 Listeria Ad546 Flour II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
35 Listeria Ad618 Soft cheese (Munster) IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
36 Listeria Ad623 Bread crumbs II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
37 Listeria Ad625 Dairy environmental sample IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
38 Listeria Ad626 Cheese (Gorgonzola) II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
39 Listeria Ad630 Cheese (Cantal) II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
40 Listeria Ad665 Milk II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog RTE food (deli salad with Adri
41 Listeria 850/109 II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes seafood) a
monocytog Adri
42 Listeria Ad1494 Strasbourg sausage II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
enes a
monocytog Adri
44 Listeria Ad1496 RT reheat (sea food) II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
102/130
Alternative method
d ult ult ult
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
(+/-) (+/-)
monocytog Adri
45 Listeria Ad1497 Pasta II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
enes a
monocytog Adri
47 Listeria Ad1679 Fish environmental sample II b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
48 Listeria Ad1781 Raw milk IV b + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
49 Listeria Ad1784 Ewe milk II a + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog Adri
50 Listeria Ad816 Uknown La + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes a
monocytog ATCC Q
51 Listeria Human Isolate 1/2 C + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 7644 Labs
monocytog ATCC Spinal Fluid of child with Q
52 Listeria 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 13932 menintoitis. Germany Labs
QL
monocytog Q
enes Labs
3
QL
monocytog Q
enes Labs
4
QL
monocytog Q
enes Labs
5
monocytog ATCC Q
56 Listeria rabbit. Cambridge. England 1/2 A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 15313 Labs
monocytog ATCC Q
57 Listeria Poultry. England 1 + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 19111 Labs
monocytog ATCC Q
58 Listeria Tissue. animal (ruminant brain) 4A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 19114 Labs
monocytog ATCC Q
59 Listeria Chicken. England 4C + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 19116 Labs
monocytog FSL Q
enes J1.049 Labs
monocytog FSL J1- Q
enes 129 Labs
monocytog FSL F6- Q
enes 367 Labs
QL
monocytog Q
enes Labs
10
monocytog ATCC Q
64 Listeria Chicken. England 4E + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 19118 Labs
103/130
Alternative method
d ult ult ult
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
(+/-) (+/-)
monocytog ATCC Q
65 Listeria Not Available N/A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 43256 Labs
monocytog ATCC Q
enes 49594 Labs
monocytog ATCC Q
enes 51772 Labs
monocytog ATCC Q
68 Listeria Diary Products (Belgium) 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 51778 Labs
monocytog ATCC51 Q
69 Listeria Dairy Products (Cheese) 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 780 Labs
monocytog ATCC51 Q
70 Listeria Dairy Products (Cheese) 3A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes 782 Labs
ATCC
monocytog Q
71 Listeria BAA- Not Available 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
751
ATCC
monocytog Q
72 Listeria BAA Not Available N/A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
2658
monocytog Q
73 Listeria BEI Li2 Q Labs Isolate BEI NR-100 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
UVM
monocytog Q
74 Listeria CWD Not Available 1/2C + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1552
UVM
monocytog Q
enes Labs
1554
UVM
monocytog Q
enes Labs
1555
UVM
monocytog Q
enes Labs
1559
UVM
monocytog Q
enes Labs
1560
UVM
monocytog Q
79 Listeria CWD Mother/Baby. Placenta 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1561
UVM
monocytog Q
80 Listeria CWD Lausanne 1987 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1563
UVM
monocytog Q
81 Listeria CWD LA Outbreak 1985 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1567
104/130
Alternative method
d ult ult ult
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
(+/-) (+/-)
UVM
monocytog Q
enes Labs
1571
UVM
monocytog Q
enes Labs
1599
UVM
monocytog Q
84 Listeria CWD Not Available 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1601
UVM
monocytog Q
enes Labs
1609
UVM
monocytog Q
enes Labs
1612
UVM
monocytog Q
enes Labs
1613
UVM
monocytog Q
88 Listeria CWD Oklahoma 1/2A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1614
UVM
monocytog Q
enes Labs
1618
UVM
monocytog Q
enes Labs
1620
UVM
monocytog Q
91 Listeria CWD Oklahoma Turkey Franks 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1626
UVM
monocytog Q
92 Listeria CWD Mother/Baby 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1627
UVM
monocytog Q
93 Listeria CWD Oklahoma Turkey Franks 1/2A + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
1629
UVM
monocytog Q
enes Labs
1630
UVM
monocytog Q
95 Listeria CWD15 Unknown 1/2C + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
53
UVM
monocytog Q
96 Listeria CWD15 Halifax 1983 4B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
74
105/130
Alternative method
d ult ult ult
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
compare
Interpreta
(+/-) (+/-)
UVM
monocytog Q
enes Labs
84
UVM
monocytog Q
98 Listeria CWD15 Not Available 3B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
enes Labs
86
UVM
monocytog Q
enes Labs
88
UVM
10 monocytog Q
Listeria CWD15 LA 1/2B + + PA IA + PA IA + PA IA + PA IA + PA IA + PA IA
0 enes Labs
89
N 100 100 100 100 100 100
PA 100 100 100 100 100 100
ND 0 0 0 0 0 0
Tot
NA 0 0 0 0 0 0
al
NA (no
0 0 0 0 0 0
growth)
PD 0 0 0 0 0 0
106/130
Annex K - Results exclusivity study - Listeria monocytogenes
NOIP: NO Organism Identification Possible

EXCLUSIVITY - Listeria monocytogenes
Alternative method
Non Target Strain
Fin Altern Fina
Final Final Final Final
al ative Alternati l Alternati Alternati Alternati Alternati
Refer metho ve ve ve ve ve
d method res method method method method
ence res result
ult
result result result
Sub Referen meth ult confir confirma confirma confirma confirma confirma
N° Species rDNA sequencing Origin Lab Final Final Final Final Final Final
od mation tion tion tion tion tion
species ce Interpr Interpret Interpret Interpret Interpret Interpret
(+/ identit identity identity identity identity identity
(+/-) (+/-) (+/-) (+/-) (+/-)
-) y of of the of the of the of the of the
strain
Smoked Adri
1 Listeria grayi Ad1198 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
salmon a
- (no
Adri NA (no NA (no
2 Listeria grayi Ad1229 Chitterling - - NA EA - NA EA - NA EA - NA EA growth - NA EA
a growth) growth)
)
-
(no - (no
3 Listeria grayi Ad1295 Spinash - - NA EA - NA EA gro - NA EA growth - NA EA
wth )
)
Adri
4 Listeria grayi Ad1296 Vegetables - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
-
(no
Adri NA (no NA (no
5 Listeria grayi Ad2415 Rillette - - NA EA - NA EA gro - NA EA - NA EA - NA EA
a growth) growth)
wth
)
Seafood Adri
6 Listeria innocua Ad 1230 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
product a
Adri
7 Listeria innocua Ad 1675 Fish - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
8 Listeria innocua Ad 1676 RT reheat - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
9 Listeria innocua Ad 1771 Ewe milk - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Pasteurized Adri
10 Listeria innocua Ad2414 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
cheese a
Dairy
Adri
11 Listeria ivanovii Ad616 environmental - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
sample
Adri
12 Listeria ivanovii Ad1288 Ewe milk - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Raw milk Adri
13 Listeria ivanovii Ad1289 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
cheese a
Adri
14 Listeria ivanovii Ad1290 Milk powder - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
15 Listeria ivanovii Ad676 Pork cheek - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
107/130
Alternative method
Non Target Strain
Fin Altern Fina
ence res result
ult
(+/-) (+/-) (+/-) (+/-) (+/-)
strain
Adri
16 Listeria seeligeri Ad2634 Trout - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
a
Environment Adri
18 Listeria seeligeri BR18 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
sample a
Adri
19 Listeria seeligeri Ad649 Cheese - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Environment Adri
20 Listeria seeligeri Ad651 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
sample a
Adri
21 Listeria welshimeri Ad1175 Fried rize - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
22 Listeria welshimeri Ad1204 Ham - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Frozen ground Adri
23 Listeria welshimeri Ad1211 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
beef meat a
Adri
24 Listeria welshimeri Ad1223 Beef meat - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
25 Listeria welshimeri Ad1228 Turkey meat - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
- (no - (no - (no
) ) )
-
(no
Adri NA (no NA (no
27 Bacillus circulans Ad734 Dairy product - - NA EA - NA EA gro - NA EA - NA EA - NA EA
a growth) growth)
wth
)
-
(no
28 Bacillus Ad741 Dairy product - - NA EA - NA EA gro - NA EA - NA EA - NA EA
s a growth) growth)
wth
)
-
(no - (no
29 Bacillus pumilus Ad733 Dairy product - - NA EA - NA EA gro - NA EA growth - NA EA
wth )
)
-
- (no (no - (no - (no - (no
30 Bacillus subtilis Ad839 Chocolate - - NA EA growth gro growth growth growth
) wth ) ) )
)
108/130
Alternative method
Non Target Strain
Fin Altern Fina
ence res result
ult
(+/-) (+/-) (+/-) (+/-) (+/-)
strain
-
- (no (no - (no - (no - (no
Enteroco casseliflavu Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
31 Ad1346 Water - - NA EA growth gro growth growth growth
ccus s a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
-
- (no (no - (no
Enteroco Adri NA (no NA (no NA (no NA (no NA (no NA (no
32 faecalis Ad 602 Raw milk - - NA EA growth gro - NA EA - NA EA growth
ccus a growth) growth) growth) growth) growth) growth)
) wth )
)
-
- (no (no - (no - (no - (no
Enteroco Pasteurised Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
33 faecium Ad177 - - NA EA growth gro growth growth growth
ccus whole egg a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
-
Enterococcus - (no (no - (no - (no - (no
Enteroco Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
34 gallinarum Ad1145 casseliflavus / Guacamole - - NA EA growth gro growth growth growth
gallinarum ) wth ) ) )
)
-
- (no (no - (no - (no
Enteroco Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
35 hirae Ad1392 Sea water - - NA EA growth gro - NA EA growth growth
) wth ) )
)
-
- (no (no - (no - (no - (no
Lactoba Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
36 acidophilus 93L822 Raw milk - - NA EA growth gro growth growth growth
cillus a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
-
- (no (no - (no - (no - (no
37 sakei Ad1150 Delicatessen - - NA EA growth gro growth growth growth
) wth ) ) )
)
-
- (no (no - (no - (no - (no
Lactococ Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
38 lactis Ad 425 Ferment - - NA EA growth gro growth growth growth
) wth ) ) )
)
109/130
Alternative method
Non Target Strain
Fin Altern Fina
ence res result
ult
(+/-) (+/-) (+/-) (+/-) (+/-)
strain
-
- (no (no - (no - (no - (no
39 plantarum Ad1147 Guacamole - - NA EA growth gro growth growth growth
) wth ) ) )
)
-
- (no (no - (no - (no - (no
Leucono Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
40 carnosus Ad386 Ham - - NA EA growth gro growth growth growth
stoc a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
-
- (no (no - (no - (no - (no
Leucono pseudomes Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
41 Ad835 Dairy desert - - NA EA growth gro growth growth growth
stoc enteroïdes a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
-
- (no (no - (no - (no
Lysiniba Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
42 sphaericus Ad872 Dairy product - - NA EA growth gro - NA EA growth growth
cillus a growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) )
)
-
- (no (no
Lysiniba Adri NA (no NA (no NA (no NA (no No No No
43 sphaericus Ad724 Lysinibacillus fusiformis Unknown - - NA EA growth gro - NA EA - NA EA
cillus a growth) growth) growth) growth) peak result result
) wth
)
-
- (no (no - (no - (no - (no
Pseudo Whole liquid Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
44 fragi Ad1327 - - NA EA growth gro growth growth growth
monas egg a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
- (no - (no - (no
Staphylo Adri NA (no NA (no No No NA (no NA (no NA (no NA (no
45 aureus Ad906 Sausage - - NA EA growth - NA EA NOIP growth growth
coccus a growth) growth) result result growth) growth) growth) growth)
) ) )
- (no - (no
Staphylo Adri NA (no NA (no NA (no NA (no
coccus a growth) growth) growth) growth)
) )
- (no
Staphylo haemolyticu Adri NA (no NA (no
coccus s a growth) growth)
)
-
- (no (no - (no - (no
Staphylo Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
48 hominis Ad849 Unknow - - NA EA growth gro - NA EA growth growth
coccus a growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) )
)
110/130
Alternative method
Non Target Strain
Fin Altern Fina
ence res result
ult
(+/-) (+/-) (+/-) (+/-) (+/-)
strain
- (no - (no - (no
Staphylo CIP81.5 Adri NA (no NA (no NA (no NA (no NA (no NA (no
coccus 8 a growth) growth) growth) growth) growth) growth)
) ) )
-
- (no (no - (no - (no - (no
Streptoc Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
50 bovis 92L613 Cheese - - NA EA growth gro growth growth growth
occus a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
Q - (no
ATCC NA (no NA (no
51 Listeria grayi Not Available Lab - - NA EA - NA EA - NA EA - NA EA - NA EA growth
700545 growth) growth)
s )
-
UVM Q (no - (no
NA (no NA (no NA (no NA (no
52 Listeria grayi LGRA00 Not Available Lab - - NA EA - NA EA gro - NA EA - NA EA growth
growth) growth) growth) growth)
1 s wth )
)
QL Q - (no
NA (no NA (no
53 Listeria grayi 030911- Industry Lab - - NA EA - NA EA - NA EA - NA EA - NA EA growth
growth) growth)
12 s )
QL Q - (no
NA (no NA (no
54 Listeria grayi 051111- Unknown Lab - - NA EA - NA EA - NA EA - NA EA - NA EA growth
growth) growth)
1 s )
QL Q - (no
NA (no NA (no
growth) growth)
2 s )
Q
UVM
56 Listeria innocua Not Available Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
167
s
Q
UVM
217
s
Q
UVM
658
s
Q
UVM
661
s
Q
UVM
662
s
ATCC Q
61 Listeria ivanovii BAA- Washing water Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
139 s
111/130
Alternative method
Non Target Strain
Fin Altern Fina
ence res result
ult
(+/-) (+/-) (+/-) (+/-) (+/-)
strain
ATCC Q
62 Listeria ivanovii BAA- Sheep fetus Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
678 s
Q
UVM
63 Listeria ivanovii Not Available Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
Liv004
s
Q
UVM
Liv005
s
Q
UVM
Liv006
s
Q
ATCC
66 Listeria seeligeri Human feces Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
11289
s
-
Q (no - (no
ATCC NA (no NA (no NA (no NA (no
67 Listeria seeligeri Not Available Lab - - NA EA - NA EA gro - NA EA - NA EA growth
11856 growth) growth) growth) growth)
s wth )
)
-
Q (no - (no
s wth )
)
QL Q
69 Listeria seeligeri 031011- Creamer Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
2 s
Intesti'/l content of Q
ATCC
70 Listeria seeligeri Clethrionomys Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
51334
glareolus s
QL Q
71 Listeria welshimeri 040713. Unknown Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
1 s
Q
ATCC
72 Listeria welshimeri Not Available Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
43548
s
Q
ATCC
73 Listeria welshimeri Soil Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
43549
s
Q
ATCC
74 Listeria welshimeri Human feces Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
43550
s
112/130
Alternative method
Non Target Strain
Fin Altern Fina
ence res result
ult
(+/-) (+/-) (+/-) (+/-) (+/-)
strain
Q
ATCC
43551
s
-
Q - (no (no - (no - (no - (no
Aeromo ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
76 hydrophila Clinical Isolate Lab - - NA EA growth gro growth growth growth
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Aerococ QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
77 viridans Raw Milk Lab - - NA EA growth gro growth growth growth
cus 17041.8 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
78 Bacillus cereus Psyllium Lab - - NA EA growth gro growth growth growth
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Burkhold ATCC Plant-derived NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
79 cepacia Lab - - NA EA growth gro growth growth growth
eria* 25416 foodstuff growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Carnoba maltaromati ATCC Vacuum- NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
80 Lab - - NA EA growth gro growth growth growth
cterium cum 43224 packed beef growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Citrobact amolanaticu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
81 Feces Lab - - NA EA growth gro growth growth growth
er s 25407 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Enterob ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
82 aerogenes Not Available Lab - - NA EA growth gro growth growth growth
acter 35029 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Enteroco ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
83 faecium Dairy Product Lab - - NA EA growth gro growth growth growth
s ) wth ) ) )
)
113/130
Alternative method
Non Target Strain
Fin Altern Fina
ence res result
ult
(+/-) (+/-) (+/-) (+/-) (+/-)
strain
-
Q - (no (no - (no - (no - (no
Escheric QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
84 coli Feces Lab - - NA EA growth gro growth growth growth
hia 11010.2 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Klebsiell ATCC Clinical isolate. NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
85 oxytoca Lab - - NA EA growth gro growth growth growth
a 43165 Califonria growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
86 Kocuria rhizophila Soil Lab - - NA EA growth gro growth growth growth
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Lactoba ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
87 casei Oral cavity Lab - - NA EA growth gro growth growth growth
cillus 11578 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
QL Q - (no (no - (no - (no - (no
Leucono mesenteroi NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
88 071011- Sauerkraut Lab - - NA EA growth gro growth growth growth
stoc des growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
7 s ) wth ) ) )
)
-
ATCC Q (no
Lysiniba NA (no NA (no
89 sphaericus BAA- / Not Available Lab - - NA EA - NA EA gro - NA EA - NA EA - NA EA
cillus growth) growth)
378 s wth
)
-
Q - (no (no - (no - (no - (no
Pseudo extremorien QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
90 Raw milk Lab - - NA EA growth gro growth growth growth
monas tialis 17041.1 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Providen QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
91 stuartii Clinical Isolate Lab - - NA EA growth gro growth growth growth
cia 11007.5 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
114/130
Alternative method
Non Target Strain
Fin Altern Fina
ence res result
ult
(+/-) (+/-) (+/-) (+/-) (+/-)
strain
-
Q - (no (no - (no - (no - (no
Rhodoco ATCC Lung abscess NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
92 equi Lab - - NA EA growth gro growth growth growth
ccus 6939 of foal growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Staphylo Seasoning NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
93 aureus 030911. Lab - - NA EA growth gro growth growth growth
coccus Powder growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
4 s ) wth ) ) )
)
-
Staphylo chromogen NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
94 17041.1 Raw milk Lab - - NA EA growth gro growth growth growth
coccus es growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
0 s ) wth ) ) )
)
-
Q - (no (no - (no - (no
Staphylo haemolyticu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
95 Human skin Lab - - NA EA growth gro - NA EA growth growth
coccus s 29970 growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) )
)
-
Q - (no (no - (no - (no - (no
Streptoc pneumonia ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
96 Not Available Lab - - NA EA growth gro growth growth growth
occus e 6302 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Streptoc Pasteurized NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
97 salivarius 011509- Lab - - NA EA growth gro growth growth growth
occus Milk growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
7 s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
parahaemol CCUG NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
98 Vibrio Sea Food Lab - - NA EA growth gro growth growth growth
yticus 43364 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Seafood NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
99 Vibrio vulnificus 021111 Lab - - NA EA growth gro growth growth growth
Product growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
B s ) wth ) ) )
)
115/130
Alternative method
Non Target Strain
Fin Altern Fina
ence res result
ult
(+/-) (+/-) (+/-) (+/-) (+/-)
strain
-
Q - (no (no - (no - (no - (no
Yersinia enterolytica Lab - - NA EA growth gro growth growth growth
s ) wth ) ) )
)
N 100 100 100 100 100 100
PA 0 0 0 0 0 0
ND 0 0 0 0 0 0
NA 100 55 50 63 53 50
To
tal NA (no
0 45 50 35 47 50
growth)
PD 0 0 0 0 0 0
No
0 0 0 2 0 0
result
116/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ative Alternati l Alternati Alternati Alternati Alternati
metho ve res ve ve ve ve
Fin d result method method result method method method
ult
Sub Referen al confir confirma confirma confirma confirma confirma
N Species rDNA sequencing Origin Lab Final Final Final Final Final Final Final Final
species ce res mation tion tion tion tion tion
Interpr Interpret Interpret Interpret result Interpret result Interpret
ult compa compare compare compare compare compare
etation ation ation ation (+/-) ation (+/-) ation
(+/ red to d to d to d to d to d to
-) identit (+/-) identity (+/-) identity (+/-) identity identity identity
strain
Smoked Adri
1 Listeria grayi Ad1198 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
salmon a
- (no
Adri NA (no NA (no
2 Listeria grayi Ad1229 Chitterling - - NA EA - NA EA - NA EA - NA EA growth - NA EA
a growth) growth)
)
-
(no - (no
3 Listeria grayi Ad1295 Spinash - - NA EA - NA EA gro - NA EA growth - NA EA
wth )
)
Adri
4 Listeria grayi Ad1296 Vegetables - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
-
(no
Adri NA (no NA (no
5 Listeria grayi Ad2415 Rillette - - NA EA - NA EA gro - NA EA - NA EA - NA EA
a growth) growth)
wth
)
Seafood Adri
6 Listeria innocua Ad 1230 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
product a
Adri
7 Listeria innocua Ad 1675 Fish - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
8 Listeria innocua Ad 1676 RT reheat - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
9 Listeria innocua Ad 1771 Ewe milk - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Pasteurized Adri
10 Listeria innocua Ad2414 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
cheese a
Dairy
Adri
11 Listeria ivanovii Ad616 environmental - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
sample
Adri
12 Listeria ivanovii Ad1288 Ewe milk - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Raw milk Adri
13 Listeria ivanovii Ad1289 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
cheese a
Adri
14 Listeria ivanovii Ad1290 Milk powder - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
15 Listeria ivanovii Ad676 Pork cheek - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
a
117/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ult
strain
Adri
a
Environment Adri
18 Listeria seeligeri BR18 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
sample a
Adri
19 Listeria seeligeri Ad649 Cheese - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Environment Adri
20 Listeria seeligeri Ad651 - - NA EA - NA EA - NA EA + FP ED - NA EA - NA EA
sample a
Adri
21 Listeria welshimeri Ad1175 Fried rize - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
22 Listeria welshimeri Ad1204 Ham - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Frozen ground Adri
23 Listeria welshimeri Ad1211 - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
beef meat a
Adri
24 Listeria welshimeri Ad1223 Beef meat - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
Adri
25 Listeria welshimeri Ad1228 Turkey meat - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
a
- (no - (no - (no
) ) )
-
(no
Adri NA (no NA (no
27 Bacillus circulans Ad734 Dairy product - - NA EA - NA EA gro - NA EA - NA EA - NA EA
a growth) growth)
wth
)
-
(no
28 Bacillus Ad741 Dairy product - - NA EA - NA EA gro - NA EA - NA EA - NA EA
s a growth) growth)
wth
)
-
(no - (no
29 Bacillus pumilus Ad733 Dairy product - - NA EA - NA EA gro - NA EA growth - NA EA
wth )
)
-
- (no (no - (no - (no - (no
30 Bacillus subtilis Ad839 Chocolate - - NA EA growth gro growth growth growth
) wth ) ) )
)
118/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ult
strain
-
- (no (no - (no - (no - (no
Enteroco casseliflavu Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
31 Ad1346 Water - - NA EA growth gro growth growth growth
ccus s a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
-
- (no (no - (no
Enteroco Adri NA (no NA (no NA (no NA (no NA (no NA (no
32 faecalis Ad 602 Raw milk - - NA EA growth gro - NA EA - NA EA growth
ccus a growth) growth) growth) growth) growth) growth)
) wth )
)
-
- (no (no - (no - (no - (no
Enteroco Pasteurised Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
33 faecium Ad177 - - NA EA growth gro growth growth growth
ccus whole egg a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
-
Enterococcus - (no (no - (no - (no - (no
Enteroco Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
34 gallinarum Ad1145 casseliflavus / Guacamole - - NA EA growth gro growth growth growth
gallinarum ) wth ) ) )
)
-
- (no (no - (no - (no
Enteroco Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
35 hirae Ad1392 Sea water - - NA EA growth gro - NA EA growth growth
) wth ) )
)
-
- (no (no - (no - (no - (no
36 acidophilus 93L822 Raw milk - - NA EA growth gro growth growth growth
) wth ) ) )
)
-
- (no (no - (no - (no - (no
37 sakei Ad1150 Delicatessen - - NA EA growth gro growth growth growth
) wth ) ) )
)
-
- (no (no - (no - (no - (no
38 lactis Ad 425 Ferment - - NA EA growth gro growth growth growth
) wth ) ) )
)
119/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ult
strain
-
- (no (no - (no - (no - (no
39 plantarum Ad1147 Guacamole - - NA EA growth gro growth growth growth
) wth ) ) )
)
-
- (no (no - (no - (no - (no
Leucono Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
40 carnosus Ad386 Ham - - NA EA growth gro growth growth growth
stoc a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
-
- (no (no - (no - (no - (no
Leucono pseudomes Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
41 Ad835 Dairy desert - - NA EA growth gro growth growth growth
stoc enteroïdes a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
-
- (no (no - (no - (no
Lysiniba Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
42 sphaericus Ad872 Dairy product - - NA EA growth gro - NA EA growth growth
cillus a growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) )
)
-
- (no (no
Lysiniba Adri NA (no NA (no NA (no NA (no No No No No No
43 sphaericus Ad724 Lysinibacillus fusiformis Unknown - - NA EA growth gro - NA EA NOIP
cillus a growth) growth) growth) growth) peak result result result result
) wth
)
-
- (no (no - (no - (no - (no
Pseudo Whole liquid Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
44 fragi Ad1327 - - NA EA growth gro growth growth growth
monas egg a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
- (no - (no - (no
Staphylo Adri NA (no NA (no No No No NA (no NA (no NA (no NA (no
45 aureus Ad906 Sausage - - NA EA growth - NA EA growth growth
coccus a growth) growth) peak result result growth) growth) growth) growth)
) ) )
- (no - (no
Staphylo Adri NA (no NA (no NA (no NA (no
coccus a growth) growth) growth) growth)
) )
- (no
Staphylo haemolyticu Adri NA (no NA (no
coccus s a growth) growth)
)
120/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ult
strain
-
- (no (no - (no - (no
Staphylo Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
48 hominis Ad849 Unknow - - NA EA growth gro - NA EA growth growth
coccus a growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) )
)
- (no - (no - (no
Staphylo CIP81.5 Adri NA (no NA (no NA (no NA (no NA (no NA (no
coccus 8 a growth) growth) growth) growth) growth) growth)
) ) )
-
- (no (no - (no - (no - (no
Streptoc Adri NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
50 bovis 92L613 Cheese - - NA EA growth gro growth growth growth
occus a growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
) wth ) ) )
)
Q - (no
ATCC NA (no NA (no
51 Listeria grayi Not Available Lab - - NA EA - NA EA - NA EA - NA EA - NA EA growth
700545 growth) growth)
s )
-
UVM Q (no - (no
NA (no NA (no NA (no NA (no
52 Listeria grayi LGRA00 Not Available Lab - - NA EA - NA EA gro - NA EA - NA EA growth
growth) growth) growth) growth)
1 s wth )
)
QL Q - (no
NA (no NA (no
53 Listeria grayi 030911- Industry Lab - - NA EA - NA EA - NA EA - NA EA - NA EA growth
growth) growth)
12 s )
QL Q - (no
NA (no NA (no
growth) growth)
1 s )
QL Q - (no
NA (no NA (no
growth) growth)
2 s )
Q
UVM
167
s
Q
UVM
217
s
Q
UVM
658
s
121/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ult
strain
Q
UVM
661
s
Q
UVM
662
s
ATCC Q
61 Listeria ivanovii BAA- Washing water Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
139 s
ATCC Q
62 Listeria ivanovii BAA- Sheep fetus Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
678 s
Q
UVM
Liv004
s
Q
UVM
Liv005
s
Q
UVM
Liv006
s
Q
ATCC
66 Listeria seeligeri Human feces Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
11289
s
-
Q (no - (no
s wth )
)
-
Q (no - (no
s wth )
)
QL Q
69 Listeria seeligeri 031011- Creamer Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
2 s
Intesti'/l content of Q
ATCC
70 Listeria seeligeri Clethrionomys Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
51334
glareolus s
122/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ult
strain
QL Q
71 Listeria welshimeri 040713. Unknown Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
1 s
Q
ATCC
43548
s
Q
ATCC
73 Listeria welshimeri Soil Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
43549
s
Q
ATCC
74 Listeria welshimeri Human feces Lab - - NA EA - NA EA - NA EA - NA EA - NA EA - NA EA
43550
s
Q
ATCC
43551
s
-
Q - (no (no - (no - (no - (no
Aeromo ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
76 hydrophila Clinical Isolate Lab - - NA EA growth gro growth growth EA growth
nas 49140 growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Aerococ QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
77 viridans Raw Milk Lab - - NA EA growth gro growth growth growth
cus 17041.8 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
78 Bacillus cereus Psyllium Lab - - NA EA growth gro growth growth growth
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Burkhold ATCC Plant-derived NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
79 cepacia Lab - - NA EA growth gro growth growth growth
eria* 25416 foodstuff growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Carnoba maltaromati ATCC Vacuum- NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
80 Lab - - NA EA growth gro growth growth growth
cterium cum 43224 packed beef growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
123/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ult
strain
-
Q - (no (no - (no - (no - (no
Citrobact amolanaticu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
81 Feces Lab - - NA EA growth gro growth growth growth
er s 25407 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Enterob ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
82 aerogenes Not Available Lab - - NA EA growth gro growth growth growth
acter 35029 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Enteroco ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
83 faecium Dairy Product Lab - - NA EA growth gro growth growth growth
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Escheric QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
84 coli Feces Lab - - NA EA growth gro growth growth growth
hia 11010.2 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Klebsiell ATCC Clinical isolate. NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
85 oxytoca Lab - - NA EA growth gro growth growth growth
a 43165 Califonria growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
86 Kocuria rhizophila Soil Lab - - NA EA growth gro growth growth growth
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Lactoba ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
87 casei Oral cavity Lab - - NA EA growth gro growth growth growth
cillus 11578 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Leucono mesenteroi NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
88 071011- Sauerkraut Lab - - NA EA growth gro growth growth growth
stoc des growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
7 s ) wth ) ) )
)
124/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ult
strain
-
ATCC Q (no
Lysiniba NA (no NA (no
89 sphaericus BAA- / Not Available Lab - - NA EA - NA EA gro - NA EA - NA EA - NA EA
cillus growth) growth)
378 s wth
)
-
Q - (no (no - (no - (no - (no
Pseudo extremorien QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
90 Raw milk Lab - - NA EA growth gro growth growth growth
monas tialis 17041.1 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Providen QL NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
91 stuartii Clinical Isolate Lab - - NA EA growth gro growth growth growth
cia 11007.5 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Rhodoco ATCC Lung abscess NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
92 equi Lab - - NA EA growth gro growth growth growth
ccus 6939 of foal growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Staphylo Seasoning NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
93 aureus 030911. Lab - - NA EA growth gro growth growth growth
coccus Powder growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
4 s ) wth ) ) )
)
-
Staphylo chromogen NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
94 17041.1 Raw milk Lab - - NA EA growth gro growth growth growth
coccus es growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
0 s ) wth ) ) )
)
-
Q - (no (no - (no - (no
Staphylo haemolyticu ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
95 Human skin Lab - - NA EA growth gro - NA EA growth growth
coccus s 29970 growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) )
)
-
Q - (no (no - (no - (no - (no
Streptoc pneumonia ATCC NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
96 Not Available Lab - - NA EA growth gro growth growth growth
occus e 6302 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
125/130
Refer
Alternative method
ence
Non Target Strain
meth
od
Altern Fina
Final Final
ult
strain
-
Streptoc Pasteurized NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
97 salivarius 011509- Lab - - NA EA growth gro growth growth growth
occus Milk growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
7 s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
parahaemol CCUG NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
98 Vibrio Sea Food Lab - - NA EA growth gro growth growth growth
yticus 43364 growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
s ) wth ) ) )
)
-
Seafood NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no NA (no
99 Vibrio vulnificus 021111 Lab - - NA EA growth gro growth growth growth
Product growth) growth) growth) growth) growth) growth) growth) growth) growth) growth)
B s ) wth ) ) )
)
-
Q - (no (no - (no - (no - (no
Yersinia enterolytica Lab - - NA EA growth gro growth growth growth
s ) wth ) ) )
)
N 100 100 100 100 100 100
PA 0 0 0 0 0 0
ND 0 0 0 0 0 0
NA 100 55 50 63 52 49
To
tal NA (no
0 45 50 35 47 50
growth)
PD 0 0 0 1 0 0
No
0 0 0 1 1 1
result
126/130
Annex L - List of collaborators
Laboratory Street ZIP Town Country

AGROLAB-Labor GmbH - GmbH - Chem.
Moosstr. 6 a 82279 Eching Germany
Untersuchungs-laboratorium - Dr. Blasy - Dr. Busse
ALS Unit 7-8 Aspen Court S60 1FB Rotherham United-Kindom
CCFRA Station Road, Chipping Campden GL55 6LD Gloucestershire, United-Kindom
Eurofins 11 rue Pierre Adolphe Bobierre 44323 Nantes France
i54 Business Park,
Eurofins WV9 5GB Wolverhampton United-Kindom
Valiant Way, Coven,
Eurofins CLF Specialised Nutrition Testing Services
Professor-Wagner-Str. 11 61381 Friedrichsdorf Germany
GmbH
GD Animal Health Arnsbergstraat 7 7400AA Deventer Netherlands
4 avenue Jean Moulin
Groupe CARSO 69633 Vénissieux Cedex France
CS 30228
Institut Pasteur, CNR &
25 rue du Docteur Roux 75724 Paris cedex 15 France
CCOMS Listeria
Institut Scientifique
Juliette Wytsmanstraat 14 1050 Brussels Belgium
de Santé Publique
Intertek Food Services GmbH - Standort Bremen Olof-Palme-Straße 8 28719 Bremen Germany
1352 avenue de Paris
LABEO 50008 Saint Lô France
CS 33608
LABOCEA Avenue de la Plage des Gueux 29000 Quimper France
Plateau technique de
LASAT microbiologie des aliments 79220 Champdeniers France
ZI Montplaisir
Max Rubner-Institut - Bundesforschungsinstitut für
E.-C.-Baumann-Str. 20 95326 Kulmbach Germany
Ernährung und Lebensmittel - Standort Kulmbach
Nederlandse Voedsel en Akkermaalsbos 2
6708 WB Wageningen Netherlands
Waren Autoriteit (VWA) (PO box 144,6700AC)
127/130
Annex M - List of strains:
List of strains tested in the inter-laboratory study
Molecular
Strains
typing
Listeria monocytogenes 2407/3139 IV b
Listeria monocytogenes 32.183 II b
Listeria monocytogenes A00C042 IV b
Listeria monocytogenes Ad253 II b
Listeria monocytogenes Ad270 IV b
Listeria monocytogenes Ad285 La
Listeria monocytogenes Ad494 II a
Listeria monocytogenes Ad816 La
Target strains
Listeria grayi Ad1504 /
Listeria grayi Ad2064 /
Listeria innocua Ad1675 /
Listeria innocua Ad1676 /
Listeria ivanovii Ad1308 /
Listeria seeligeri Ad2634 /
Listeria seeligeri Ad2637 /
Listeria welshimeri Ad1175 /
Bacillus pumilus Ad733 /
Bacillus thuringiensis Ad2067 /
Enterococcus mundtii Ad1365 /
Non-target Enterococcus faecalis Ad547 /
strains Lactococcus lactis Ad425 /
Leuconostoc pseudomesenteroïdes Ad835 /
Staphylococcus hominis Ad849 /
Staphylococcus pasteurii Ad1717 /
128/130
Annex N - Listeria spp. and Listeria monocytogenes
testing at the genus level
Interlaboratory Study
Cryobead of already characterized strain
Expert Lab. 
TSA
Streaking onto TSYEA Streaking onto

O&A and Oxford
and/or RAPID’L.mono
Incubation for 24 h ± 2 h
at 37°C ± 1°C Incubation for 24 h to 48 h
at 37°C ± 1°C
Collaborator
One colony per plate
Confirmation by using
Confirmation by the tests
the Direct Transfer Procedure
described in the reference
of the Biotyper of BRUKER
method
on fresh culture
129/130
Annex O - Raw data inter-laboratory study
See Excel File: 2017LR75 Microval MCS AND ILS Listeria spp and mono (Version 1).xlsx; Tab «Raw data ILS- Lspp» and «Raw data
ILS- Lmono »
130/130

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MicroVal Study 2017LR75: Confirmation Method

Validation study of the MALDI Biotyper® Complete Solution as an Alternative

MCS ILS, Version 2

Report issued and authorized by:

Scope of validation: confirmation of Listeria spp. and Listeria monocytogenes

Certification organization: Lloyd's Register

1.1 Reference method 6

1.2 Alternative method 6

1.2.1 Instrumentation Workflow and Mechanism 7

1.2.2 Calculating a log (score) value and interpretation 8

1.2.3 Instrumentation Features 10

1.2.4 Software Features 11

1.2.5 Targets and biotargets 11

1.2.6 Associated devices 11

1.3 Study design 12

2 Method comparison study 12

2.1 Selection of Test Strains 12

2.2 Samples to be tested 13

2.3 Inclusivity and exclusivity 13

2.3.1 Data discrepancy 14

2.3.2 Listeria spp. testing at the genus level 14

2.3.3. Summary of the inclusivity and exclusivity testing 15

2.3.3 Expression of results 15

Protocol and Materials 29

Statistics and Results 30

6 Conclusions of Interlaboratory Study 40

Annex B - Flow diagram of the alternative method 44

Annex B - Kit inserts 46

Annex C – Certificate of equivalence 55

Annex D – Listeria spp. and Listeria monocytogenes testing 56

Annex E - Raw data inclusivity study 57

Annex F - Raw data exclusivity study 58

Annex G - Results inclusivity study: 59

Annex H - Results exclusivity study- Listeria spp. 79

Annex I - Results inclusivity study - Listeria monocytogenes 95

Annex K - Results exclusivity study - Listeria monocytogenes 107

Annex L - List of collaborators 127

Annex O - Raw data inter-laboratory study 130

The MBT utilizes Matrix Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-

Reference method: ISO 11290:2017

1.1 Reference method

See the flow diagram in Annex A.

1.2 Alternative method

See the flow diagram of the alternative method in Annex B.

See the link below corresponding to clinical applications.

1.2.1 Instrumentation Workflow and Mechanism

Strains were analyzed using MALDI-TOF MS. A laser in the MALDI-TOF MS

The MALDI Biotyper (MBT) software and library:

1.2.2 Calculating a log (score) value and interpretation

Isolated colonies on appropriate medium

Perform Direct Transfer (DT).

Release the results for

Repeat by running DT, Repeat by running DT,

Release the results for

Streak onto non selective Streak onto non selective

1.2.3 Instrumentation Features

Table 1. A Comparison of the laser technology and the vacuum system

MALDI Biotyper System MALDI Biotyper Smart System

Vacuum system High-performance vacuum system