This document outlines the topics to be covered in an Instrumental Methods of Analysis course, including various spectroscopic techniques like UV-Vis spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and atomic absorption spectroscopy. It also covers topics in chromatography, such as paper chromatography, gas chromatography, high performance liquid chromatography, ion exchange chromatography, gel chromatography, affinity chromatography, and electrophoretic techniques like gel electrophoresis. The document provides an overview of key concepts, principles, instrumentation and applications to be examined for each instrumental analysis technique.
This document outlines the topics to be covered in an Instrumental Methods of Analysis course, including various spectroscopic techniques like UV-Vis spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and atomic absorption spectroscopy. It also covers topics in chromatography, such as paper chromatography, gas chromatography, high performance liquid chromatography, ion exchange chromatography, gel chromatography, affinity chromatography, and electrophoretic techniques like gel electrophoresis. The document provides an overview of key concepts, principles, instrumentation and applications to be examined for each instrumental analysis technique.
This document outlines the topics to be covered in an Instrumental Methods of Analysis course, including various spectroscopic techniques like UV-Vis spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and atomic absorption spectroscopy. It also covers topics in chromatography, such as paper chromatography, gas chromatography, high performance liquid chromatography, ion exchange chromatography, gel chromatography, affinity chromatography, and electrophoretic techniques like gel electrophoresis. The document provides an overview of key concepts, principles, instrumentation and applications to be examined for each instrumental analysis technique.
This document outlines the topics to be covered in an Instrumental Methods of Analysis course, including various spectroscopic techniques like UV-Vis spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and atomic absorption spectroscopy. It also covers topics in chromatography, such as paper chromatography, gas chromatography, high performance liquid chromatography, ion exchange chromatography, gel chromatography, affinity chromatography, and electrophoretic techniques like gel electrophoresis. The document provides an overview of key concepts, principles, instrumentation and applications to be examined for each instrumental analysis technique.
1. Explain Lambert-Beer Law. Discuss Deviation of Lambert-Beer Law.
2. Explain: Chromophore, Auxochrome, Bathochromic shift and hyperchromic effect. 3. Explain analysis of binary mixtures of absorbing substances by simultaneous equation method. 4. Write down the theory of fluorescence and phosphorescence spectroscopy. 5. Explain various factors affecting fluorescence spectroscopy. 6. Explain the principle and different types of vibration in IR Spectroscopy. 7. Explain various factors affecting IR spectroscopy. 8. Explain various Sampling techniques of IR Spectroscopy. 9. Explain the principle, instrumentation and applications of Flame Photometry. 10. Explain in detail flame and nebulizer burner system in flame photometry. 11. Discuss about the interferences in AAS 12. Explain the basic principle of atomic spectroscopy. Give the difference between the atomic absorption and atomic emission spectroscopy. 13. Explain in detail theories of chromatography. 14. Write a detail note on Paper Chromatography. 15. Write a note on Gel Electrophoresis or Zone Electrophoresis. 16. Write a detail note on Iso-electric focusing technique. 17. Write a detail note on principle and instrumentation of Gas chromatography. 18. Explain GC-MS 19. Write a note on principle, instrumentation and application of HPLC. 20. Explain about Stationary phase and mobile phase used in HPLC. 21. Write a short note on Ion exchange chromatography. 22. Explain about separation mechanism and application of Ion exchange chromatography. 23. Write a short note on Gel chromatography or Size exclusion Chromatography. 24. Explain about different type of stationary phase or gel used in Gel Chromatography. 25. Write a short note on Affinity chromatography.