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Worksheet 15-ENZYMES

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Şişli

Terakki High School


2020 – 2021 Academic
Year IB - Diploma
Programme
st
1 term 9Pre- IB Biology –Worksheet 15-
Enzymes

Name-Surname Ekim Başaran Class 9H No 1080

1.Define the terms “catalyst” and “activation energy”.

1)a substance that speeds up the chemical reaction enzymes is biological catalysts.
2)is the amount of energy to make the reactions happen minimum required.

2. Distinguish between cofactors and coenzymes.

The key difference between coenzyme and cofactor is that the coenzymes are organic
molecules, whereas the cofactors can be either organic or inorganic molecules.

3. Many factors can affect the rate of enzyme action. Explain each factor listed here

a. initial concentration of substrate The more substrate molecules that are

available, the more frequently they access the active sites of the enzyme molecules

b. pH every enzyme works best at specific ph


c. Temperature Every enzyme works at different temperature for the human body.

4. Define and describe the effect of “denaturation” in reference to enzymes.

At high temperatures, most of the enzymes change their shape and become non-functional. It
is irascible.

5. Fill in the blanks for the following:

a. Reactions that involve breaking big molecules into smaller molecules release energy and
are called ________catabolic__________________
b. Reactions that involve making bigger molecules out of smaller ones molecules use up
energy and are called ______anobolic________________
c. The main source of energy for all life is the ____the sun_________________
d. Reactions are controlled by the presence or absence of particular
_____enzyme_____________.
e. Enzymes are biological ___catalyzs___________ made by all living cells.

f. A metabolic pathway begins with a particular ___substrate_______, terminates with an


end ____product__________, and has many minute steps in between. A reactant is a
substance that participates in a ___reaction______. A product is a substance that is
___produced_________ by the reaction. The reactants in an enzymatic reaction are
called the ___substrate__________ for that enzyme.

g. An ____enzyme_______ is a protein molecule that functions as an organic catalyst to


lower the ___abservation energy_________________ of a chemical reaction.
h. The energy that must be added to cause molecules to react with one another is called
_______activation_______________.
i. When an enzyme forms a complex with its substrate, the part of the enzyme that combine
with the substrate is called the ______abservation energy______________.

6.Label the parts on this diagram


7. Which graph below best represents a graph of the enzyme activity vs temperature?
Explain what is occurring at each portion of the graph.

2
8. An experiment was carried out to investigate the action of the enzyme catalase from liver
cells. Three test tubes were set up as shown under same and optimum environmental
factors:

The comparison of the reaction rate was found to be III > II > I.

State which factor (substrate concentration, enzyme concentration and surface of the
substrate) had an effect over reaction rate and explain how it affected.

9. Water, Oil X, and an enzyme that breaks down oil X are put in a test tube.
I. Fatty acids
II. Amino acids
III. Glycerol
IV. Glucose
V. Enzyme that breaks down oil X

After a while, which of the above compounds are found in the test tube?

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