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    4.8.03 AOAC of Fi Cial Method 927.02 Cal Cium in An I Mal Feed

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    estefania
    This document provides a dry ash method for determining the calcium content in animal feed using titration with potassium permanganate. The method involves: 1) Igniting a weighed sample to ash and dissolving the residue in hydrochloric and nitric acids. 2) Titrating an aliquot of the solution with ammonium hydroxide and hydrochloric acid to adjust the pH. 3) Precipitating calcium as calcium oxalate by adding ammonium oxalate. 4) Filtering and washing the precipitate, then redissolving and titrating with potassium permanganate to determine the calcium content.

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    4.8.03 AOAC of Fi Cial Method 927.02 Cal Cium in An I Mal Feed

    Uploaded by

    estefania
    523 views1 page
    This document provides a dry ash method for determining the calcium content in animal feed using titration with potassium permanganate. The method involves: 1) Igniting a weighed sample to ash and dissolving the residue in hydrochloric and nitric acids. 2) Titrating an aliquot of the solution with ammonium hydroxide and hydrochloric acid to adjust the pH. 3) Precipitating calcium as calcium oxalate by adding ammonium oxalate. 4) Filtering and washing the precipitate, then redissolving and titrating with potassium permanganate to determine the calcium content.

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    927.02

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    This document provides a dry ash method for determining the calcium content in animal feed using titration with potassium permanganate. The method involves: 1) Igniting a weighed sample to ash and dissolving the residue in hydrochloric and nitric acids. 2) Titrating an aliquot of the solution with ammonium hydroxide and hydrochloric acid to adjust the pH. 3) Precipitating calcium as calcium oxalate by adding ammonium oxalate. 4) Filtering and washing the precipitate, then redissolving and titrating with potassium permanganate to determine the calcium content.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    523 views1 page

    4.8.03 AOAC of Fi Cial Method 927.02 Cal Cium in An I Mal Feed

    Uploaded by

    estefania
    This document provides a dry ash method for determining the calcium content in animal feed using titration with potassium permanganate. The method involves: 1) Igniting a weighed sample to ash and dissolving the residue in hydrochloric and nitric acids. 2) Titrating an aliquot of the solution with ammonium hydroxide and hydrochloric acid to adjust the pH. 3) Precipitating calcium as calcium oxalate by adding ammonium oxalate. 4) Filtering and washing the precipitate, then redissolving and titrating with potassium permanganate to determine the calcium content.

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    © All Rights Reserved
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    of 1

    4.8.

    03
    AOAC Official Method 927.02
    Calcium in Animal Feed
    Dry Ash Method
    First Action 1927
    Final Action 1945
    (Applicable to mineral feeds only.)
    Weigh 2 g finely ground test portion into SiO2 or porcelain dish
    and ignite in furnace to C-free ash, but avoid fusing. Boil residue in
    40 mL HCl (1 + 3) and few drops HNO3. Transfer to 250 mL
    volumetric flask, cool, dilute to volume, and mix thoroughly. Pipet
    25 mL clear liquid into beaker, dilute to ca 100 mL, and add 2 drops
    methyl red, 984.13B(c) (see 4.2.09). Add NH4OH (1 + 1) dropwise
    to pH 5.6, as shown by in ter me di ate brown ish-orange. If
    overstepped, add HCl (1 + 3) with dropper to orange. Add 2 more
    drops HCl (1 + 3). Color should now be pink (pH 2.5–3.0), not
    orange. Dilute to ca 150 mL, bring to boil, and slowly add, with
    con stant stir ring, 10 mL hot sat u rated (4.2%) so lu tion of
    (NH4)2C2O4. If red changes to orange or yellow, add HCl (1 + 3)
    dropwise until color again changes to pink. Let stand overnight for
    precipitate to settle. Filter supernate through quantitative paper,
    Gooch, or fritted glass filter (fine Pyrex is preferable), and wash
    precipitate thoroughly with NH4OH (1 + 50). Place paper or crucible
    with precipitate in original beaker, and add mixture of 125 mL H2O
    and 5 mL H2SO4. Heat to 70°C and titrate with 0.02M KMnO4,
    940.35 (see A.1.10), to first slight pink. Presence of paper may cause
    color to fade in few s. Correct for blank and calculate percent Ca.

    1 mL 0.02M KMnO4 = 2.004 mg Ca


    References: JAOAC 10, 177(1927); 19, 93, 574(1936);
    28, 80(1945).

    CAS-7440-70-2 (calcium)

    ã 2008 AOAC INTERNATIONAL

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