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    Komar University of Science and Technology Practical General Molecular Biology Lab Section (L-3)

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    Rasan Qadr
    This document summarizes a student laboratory experiment on red blood cell fragility testing. It includes the names and codes of 4 students, an introduction on osmotic fragility testing, the aim to detect fragile red blood cells, a list of materials used, and the method of mixing blood samples with saline solutions of varying concentrations and examining under a microscope. The results show photos of red blood cells in hypertonic, isotonic, and hypotonic solutions. The discussion analyzes how cells appear shrunken, normal-shaped, or swollen in the different solutions based on water diffusion.

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    Komar University of Science and Technology Practical General Molecular Biology Lab Section (L-3)

    Uploaded by

    Rasan Qadr
    22 views5 pages
    This document summarizes a student laboratory experiment on red blood cell fragility testing. It includes the names and codes of 4 students, an introduction on osmotic fragility testing, the aim to detect fragile red blood cells, a list of materials used, and the method of mixing blood samples with saline solutions of varying concentrations and examining under a microscope. The results show photos of red blood cells in hypertonic, isotonic, and hypotonic solutions. The discussion analyzes how cells appear shrunken, normal-shaped, or swollen in the different solutions based on water diffusion.

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    This document summarizes a student laboratory experiment on red blood cell fragility testing. It includes the names and codes of 4 students, an introduction on osmotic fragility testing, the aim to detect fragile red blood cells, a list of materials used, and the method of mixing blood samples with saline solutions of varying concentrations and examining under a microscope. The results show photos of red blood cells in hypertonic, isotonic, and hypotonic solutions. The discussion analyzes how cells appear shrunken, normal-shaped, or swollen in the different solutions based on water diffusion.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    22 views5 pages

    Komar University of Science and Technology Practical General Molecular Biology Lab Section (L-3)

    Uploaded by

    Rasan Qadr
    This document summarizes a student laboratory experiment on red blood cell fragility testing. It includes the names and codes of 4 students, an introduction on osmotic fragility testing, the aim to detect fragile red blood cells, a list of materials used, and the method of mixing blood samples with saline solutions of varying concentrations and examining under a microscope. The results show photos of red blood cells in hypertonic, isotonic, and hypotonic solutions. The discussion analyzes how cells appear shrunken, normal-shaped, or swollen in the different solutions based on water diffusion.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 5

    Komar university of science and technology

    Practical General molecular biology

    Lab section (L-3)

    Red blood cell fragility test

    Name & code:


    1. Rasan Qadir F201475

    2. Nma Soran F201546

    3. Aya Saman F200540

    4. San Burhan F200931

    Submission date (8/11/2021)

    1 | Page
    Introduction

    The osmotic fragility test (OFT) is a method of determining erythrocyte

    resilience to hemolysis when they are exposed to varied amounts of

    saline solution dilution. There is no actual agreement among

    laboratories on a reference range of results for erythrocyte osmotic

    fragility.

    Aim

    Osmotic fragility is a blood test to detect whether red blood cells

    are more likely to break down. Erythrocyte fragility refers to the

    propensity of erythrocytes (red blood cells, RBC) to hemolyze

    (rupture) under stress.

    2 | Page
    Material
    1-Microscope

    2-Slide

    3- Humam blood

    4-oil

    5-Nacl

    6-tube

    7- centrifuge

    3 | Page
    Method
    First, We took out blood from our friends arm,. Following that, we mix the blood

    with two different rates of sodium chloride in two different tubs, then mix with

    distilled water (no salt) and wait a short time. It is important to be careful not to

    shake the tubs with our hands to avoid destroying red blood cells. The three tubs

    were then placed in a centrifuge to separate the blood components. After

    extracting the liquids from the tubs, we put the remaining tub parts into a sample.

    Finally, we examine a sample under a microscope to determine osmotic and

    environmental parameters at various levels of the cell membrane in a saline

    solution environment.

    Result

    hypertonic RBC 100x hypotonic RBC 100x Isotonic RBC 100x

    4 | Page
    Discussion

    In this discussion we want to talk about those picture that we can see it

    clearly. The first experience is hypertonic red blood cell, and light

    micrograph of red blood cells in a hypertonic solution. Hypertonic

    solutions contain a high concentration of solute relative to the cells'

    cytoplasm. When the cells are placed in a hypertonic solution, the water

    diffuses out of the cells, causing them to shrink and collapse. The

    second experience is isotonic solutions are those where the

    concentration of solute is the same as that found in the cells' cytoplasm.

    When the cells are placed in an isotonic solution, they retain their

    normal shape and water content. The third experience is hypotonic

    solution which contain a low concentration of solute relative to the cells'

    cytoplasm. When the cells are placed in a hypotonic solution, the water

    diffuses into the cells, causing them to become turgid.

    5 | Page

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