Mejmbps Vol11 No3 June2020 - 19
Mejmbps Vol11 No3 June2020 - 19
Mejmbps Vol11 No3 June2020 - 19
MEDITERRANEAN PUBLICATION
AND RESEARCH INTERNATIONAL
INTERNATIONAL
INTERNATIONALJOURNAL
MEDICAL,
VOL. 11 NO.3
JOURNALOF– MBP
BIOLOGICAL AND
JUN-2020 PHAR.
ISSN: SCI.
1999-5650
VOL. 11 NO.3 JUN-2020 ISSN: 1999-5650
E
rythrocytes are important in the transport (RBCs).Chicken's RBCs
of oxygen to the tissues in the body. The possessed highest level
need to preserve the integrity of of fragility, surpassing all.
erythrocytes played by their membranes is Conclusively, there are
enormous.This work studied erythrocytes varying levels of fragility
fragility in some vertebrates (normal human cell, among vertebrates
human sickle cell, cow, sheep, goat and chicken), (erythrocytes).
INTRODUCTION
E
rythrocytes are one of the vital components of blood in the biological
system. They are composed of the haemoglobin (I.e oxygen carrying
pigment that is parcel of the blood). Haemoglobin (situated in the
red cells) is responsible for distribution of oxygen ( the oxidizing agent in
227 EDITOR-IN-CHIEF
Prof. A.S. Hossein [Saskatchewan]
MEDITERRANEAN PUBLICATION INTERNATIONAL JOURNAL – MBP
AND RESEARCH INTERNATIONAL VOL. 11 NO.3 JUN-2020 ISSN: 1999-5650
228 EDITOR-IN-CHIEF
Prof. A.S. Hossein [Saskatchewan]
MEDITERRANEAN PUBLICATION INTERNATIONAL JOURNAL – MBP
AND RESEARCH INTERNATIONAL VOL. 11 NO.3 JUN-2020 ISSN: 1999-5650
be applicable to birds.While this test has been known since around 1920s, to
date the data measuring the fragility of red cells in vertebrates is very
limited [8]. This study will help in filling the knowledge gap.It main objective
was to measure the specific fragility of some vatebrate cells (normal human
cell, sickle human's cell, cow, sheep, goat and chicken .
Blood samples
The blood samples used include:
1) Sickle cell blood was collected from Sickle cell patient in Gwadabawa,
Sokoto state, Nigeria in EDTA container.
2) Normal human blood was collected in Gwadabawa, Sokoto state,
Nigeria by venipuncture in EDTA container.
3) Blood sample from goat, sheep and cow were collected in
Gwadabawa veterinary clinic (Sokoto state, Nigeria) through jugular
vein in EDTA container.
4) Blood sample from chicken was collected after slaughtering and
drived in EDTA container[9,10] .
Analytical Methods
Principle: Test and normal red cells were placed in a series of graded-
strength sodium chloride solutions and any resultant haemolysis is
compared with a 100%.
Procedure:
Twenty micro litre of fresh blood from normal human, sickler human , cow,
sheep, goat and chicken each were placed in a set of three test tubes
229 EDITOR-IN-CHIEF
Prof. A.S. Hossein [Saskatchewan]
MEDITERRANEAN PUBLICATION INTERNATIONAL JOURNAL – MBP
AND RESEARCH INTERNATIONAL VOL. 11 NO.3 JUN-2020 ISSN: 1999-5650
Statistical Analysis
The results obtained were analysed using one-way ANOVA by instat3
software (Version: San Diego, USA) and presented as mean ± Standard Error
of mean.Difference between means are considered significant at p<0.05.
RESULTS
Table1 : Fragility of the erythrocytes of some vertebrates
Percentage haemolysis in NaCl solution
S/No. Organism 0.30 NaCl 0.40 NaCl 0.50 NaCl
category Solution Solution Solution
1 Human normal 40.00±0.01% 36.00±0.05% 34.26±0.04
cell
2 Human sickle 78.00±1.28% 74.00±4.20% 72.00±0.80%
cell
3 Cow 96.00±0.68% 94.00±0.68% 88.00±0.02%
4 Sheep 89.29±0.02% 85.29±1.70% 79.29±2.87%
5 Goat 80.00±1.02% 74.00±4.20% 72.00±0.80%
6 Chicken 132.00±2.28% 124.00±2.40% 116.00±1.45%
All results are expressed as mean ± standard error of mean, % = percentage
haemolysis (fragility).
DISCUSSION
The erythrocyte is well-equipped with a specialized cytoskeleton providing
membrane with adequate support.The essence of fragility test is to see at
which concentration haemolysis start and at which it become
230 EDITOR-IN-CHIEF
Prof. A.S. Hossein [Saskatchewan]
MEDITERRANEAN PUBLICATION INTERNATIONAL JOURNAL – MBP
AND RESEARCH INTERNATIONAL VOL. 11 NO.3 JUN-2020 ISSN: 1999-5650
231 EDITOR-IN-CHIEF
Prof. A.S. Hossein [Saskatchewan]
MEDITERRANEAN PUBLICATION INTERNATIONAL JOURNAL – MBP
AND RESEARCH INTERNATIONAL VOL. 11 NO.3 JUN-2020 ISSN: 1999-5650
[18]
.Cow,sheep,and goat might have elevated % fragility because,they need
not to conserve much water compared to the camel ,who has much osmotic
resistance in a bid suitable for specific water interaction behaviour [19,20,21] .
CONCLUSION
The vertebrates (human normal red cells, human sickle cells, cow, sheep,
goat and chicken) examined in this study possessed varying degree of
erythrocytes fragility.
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232 EDITOR-IN-CHIEF
Prof. A.S. Hossein [Saskatchewan]
MEDITERRANEAN PUBLICATION INTERNATIONAL JOURNAL – MBP
AND RESEARCH INTERNATIONAL VOL. 11 NO.3 JUN-2020 ISSN: 1999-5650
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233 EDITOR-IN-CHIEF
Prof. A.S. Hossein [Saskatchewan]