ICAR DT Syllabus

NEW AND RESTRUCTURED
POST-GRADUATE CURRICULA & SYLLABI
Dairy Science & Technology

Dairy Technology
Dairy Microbiology
Dairy Chemistry
Dairy Engineering
Education Division
Indian Council of Agricultural Research
New Delhi
April 2009
Contents
Page(s)
Executive Summary 3-4

BSMAC Composition 5
Preamble 6-7
Organization of Course Contents & Credit Requirements 8
Dairy Technology 9-30

Course Structure at a Glance 9
Course Contents 10
List of Journals 28
Suggested Broad Topics for Master’s and Doctoral Research 29
Dairy Microbiology 31-52

Course Contents 32
List of Journals 47
Dairy Chemistry 53-67

Course Contents 54
List of Journals 66
Dairy Engineering 68-85

Course Contents 69
Pre-requisite courses 79
List of Journals 83
Compulsory Non-Credit Courses 86-90
2
EXECUTIVE SUMMARY
The salient features of these new syllabi of the four major Dairy Processing
disciplines of Dairy Chemistry, Dairy Engineering, Dairy Microbiology and Dairy
Technology developed by the Broad Subject Matter Area Committee on Dairy Science &
Technology are summarized hereunder.
Dairy Technology
• A new course introduced on “Traditional and value-added dairy products” thereby

elevating the traditional dairy products to the PG programs.
• Earlier course “Dairy Processing-III’ restructured into “Membrane technology in

Dairy Processing”, and “Dairy Processing-II” revised to two updated courses viz.,
“Alternative processes for the dairy & food industries” and “Technology of
Emulsions, foams and gels”, both having practical components.
• The doctoral-level course “Product-Process Monitoring” has been revamped with

respect to the title and contents. This “Product monitoring & process control”
course gives an insight into the novel analytical and processing dimensions of the
modern food industry.
• The new courses introduced at master’s level will require additional infrastructure
in terms of equipment for conducting the practical classes, without which it will
end up being the same as it was under the old syllabus.
• Also, HRD training in the areas of new technologies and new instrumental
techniques will be indispensable in implementation of the new curricula.
Dairy Microbiology
• Biotechnological applications in dairy processing with special reference to

molecular biology particularly focused in the revised courses.
• ‘Microbial diversity and physiology” and “Advanced microbial genetics” are the
re-designed courses meant to address the recent development in the rapidly
advancing field.
• Food-borne pathogens and Microbial quality & safety aspects are the re-structured
courses to take care of the emerging needs of the dairy & food industries.
• New biotechnological tools being essential in implementing the new syllabi,

substantial, additional funds will have to be made available.
Dairy Chemistry
• Recent advances in the area of chemistry of milk constituents have been

incorporated in the revised syllabi.
3
• Bioactive role of milk constituents in relation to dairy ingredients in health foods
has been the stressed in the new curricula.
• New analytical techniques, particularly rapid test kits and bio-sensors are among
the salient novel topics introduced in the syllabus.
• In order to effectively put the new curricula in teaching practice both the increased
HRD and financial/infrastructural support requirement will become unavoidable.
Dairy Engineering
• While all the courses have been extensively revised to include recent
developments, “Bio-thermal process engineering” represents a re-look at the
emerging processing needs of the dairy and food industries.
• Simulation in Dairy and Food Engineering addresses the fast developing areas in
the wake of the progress being made in the Computing Science.
• Newer developments in the area of electronics as applied to industrial

instrumentation are crucial to the all-important process control, and this has been
appropriately covered in the revised course contents.
• The Instrumental experiments will necessitate enhanced funds made available for
HRD training and procurement of new equipment.
4
BSMA Committee on Dairy Science & Technology
(Dairy Technology/Dairy Engineering/Dairy Microbiology/Dairy Chemistry)
(Constituted by ICAR vide Office order No. F. No. 13 (1)/2007- EQR

dated January 14, 2008)
Name & Designation Address Specialization

Dr. G. R. Patil National Dairy Research Dairy Sciences
Jt. Director (Acad.) Institute, Karnal
Convener
Dr. G. S. Bhat Dairy Science College, Dairy
Dean Karnataka Veterinary, Animal Chemistry
& Fishery Sciences University,
Hebbal, Bangalore
Dr. A. K. Misra Faculty of Dairy Technology, Dairy
Dean West Bengal University of Microbiology
Animal & Fishery Sciences,
Mohanpur
Dr. B. P. Shah Faculty of Dairy Science Dairy
Dean SMC College of Dairy Science, Engineering
Anand Agril. Univ., Anand
Prof. I. K. Sawhney Dairy Engineering Division Dairy
Principal Scientist National Dairy Research Engineering
Institute, Karnal
Dr. J.B. Prajapati Dairy Microbiology Division Dairy

Prof. & Head SMC College of Dairy Science, Microbiology
Dr. P.S. Prajapati Dairy Technology Division Dairy

Head SMC College of Dairy Science, Technology
Dr. A.A. Patel Dairy Technology Division Dairy
Head National Dairy Research Technology
Member Secretary Institute, Karnal
5
PREAMBLE
The concerted efforts made by different dairy development agencies in the country
over the past four decades have led India to emerge as the largest milk producing nation in
the world. About 14% of nearly 100 million tones of milk produced annually (i.e. approx.
38 million liters of milk everyday) is channeled through the organized dairy sector. Thus,
the ever increasing amount of milk handled by the organized sector requires that the
industry be continually provided with quality manpower to meet its growing needs.
Further, the increasing R & D requirement of the sector places a particular responsibility
on the academic institutions involved in manpower development at the post-graduate
level.
More and more institutions at national or state levels are offering post-graduate
programs on the four major disciplines of Dairy Processing viz., Dairy Technology, Dairy
Engineering, Dairy Chemistry and Dairy Microbiology. Therefore, a uniform approach in
various aspects of these programs including the syllabi would be highly desirable.
Further, since there are continuing developments in the area of Dairy Processing research,
it is necessary to update the PG curricula as frequently as necessary. The last revision of
the Dairy Processing syllabi at the ICAR level took place about a decade ago. Hence, a re-
look at the existing syllabi had become due.
The Indian Council of Agricultural Research, therefore, felt it necessary to
constitute a National Core Group (NCG) under the chairmanship of Dr. J.C. Katyal, Vice
Chancellor, CCSHAU, Hisar with the following objectives:
1. To define names and curricula of Master’s and Ph.D. programmes in different
disciplines, and
2. To re-write the syllabi of the PG courses offered in various disciplines.
On the recommendations of the NCG, the Council constituted 18 Broad Subject

Matter Area (BSMA) Committees for assisting the Group in restructuring the PG course
curricula. The BSMA Committee on Dairy Science & Technology (vide Appendix-I) was
constituted to have the nation’s major academic institutions offering PG courses in Dairy
Processing well represented.
This committee, like other BSMA committees, was required to fulfill its mandate
of re-writing the course curricula in Dairy Chemistry, Dairy Engineering, Dairy
Microbiology and Dairy Technology, through interactions at various levels and among
different groups including stakeholders. The inputs thus provided by the Committee to the
NCG would enable the Group to make appropriate recommendations to the ICAR for a
uniform system of PG education in the area of Dairy Processing.
In the process of achieving the NCG’s objectives, a meeting of the Conveners and
Member-Secretaries of various BSMA committees was held with Dr. J.C. Katyal in the
Chair, on February 14, 2008 in New Delhi, wherein various aspects of restructuring the
PG syllabi in different disciplines were discussed in depth and some broad guidelines were
developed for all the Committees to follow.
The Committee on DS&T made intense efforts to develop the revised curricula in
the four Dairy Processing disciplines. In order to fulfill its mandate the Committee
followed the under-mentioned course of action.
A. The Committee members (Convener, a Member and Member-Secretary) at NDRI

carried out the following preliminary exercise (January 7-11, 2008):
1. A preliminary appraisal of the current PG curricula in Dairy Processing disciplines
of the NDRI University.
6
2. Working out of a unified approach with regard to the course structure (broad
framework for course classification) and minimum credit hours based on the
deliberations that took place in the BSMA meeting held on 18th Nov, 2007.
3. Identified experts (Appendix-II) in the academics and industry who could be
invited to give their comments on the subject.
B. The PG curricula were sent to experts in various Dairy processing disciplines for their
comments (March, 2008).
C. The syllabi (individual course contents) were also sent to subject matter specialists in
Dairy Technology, Dairy Engineering, Dairy Chemistry and Dairy Microbiology
together with a copy of a specimen of the revised “Organization of Course contents”
for suitable revision. Feedback was received from most respondents following which a
2-day workshop was organized on 21st & 22nd April 2008 which was attended by the
Committee members and senior scientists (Appendix-III) in different dairy processing
disciplines at NDRI, Karnal.
D. The finalized syllabi were received by the Member-Secretary from the four discipline-
Heads and fine-tuned by the Committee members located at Karnal (May-June, 2008).
The recommendations thus emerging from this process were discussed in the
meeting of the Member-Secretaries of various BSMA committees held on 12th June 2008
at HAU Hisar. Subsequently, the syllabi were given final shape in meeting of the NCG
and the BSMA Conveners and Member-Secretaries held at NASC, New Delhi on June 23-
24, 2008.
The restructured curricula have several new features in terms of the organization of
course contents as well as the course contents itself. Emphasis has been laid on emerging
technologies such as molecular biology applications in dairy processing, new processing
technologies e.g., electric pulse treatment, microwave processing, etc. - a new course has
been introduced on these topics. Role of milk constituents as ingredients for health and
special foods has been focused in view of the recent developments in the fundamental and
applied aspects in this area.
It is hoped, when implemented, the revised curricula will prove meaningful in
meeting the requirement of the concerned institutions for a uniform pattern of post-
graduate teaching and research so that the objective of providing quality manpower for
industrial and institutional dairy establishments can be attained. It will certainly help the
Dairy Processing academic programs keep abreast of the frontier areas of research the
world over.
7
ORGANIZATION OF COURSE CONTENTS
&
CREDIT REQUIREMENTS
Code Numbers
• All courses are divided into two series: 500-series courses pertain to Master’s level,
and 600-series to Doctoral level. A Ph. D. student must take a minimum of two 600
series courses, but may also take 500-series courses if not studied during Master’s
programme.
• Credit seminar for Master’s level is designated by code no. 591, and the two seminars
for Doctoral level are coded as 691 and 692, respectively.
• Similarly, 599 and 699 codes have been given for Master’s research and Doctoral
research, respectively.
Course Contents
The contents of each course have been organized into:
• Objective – to elucidate the basic purpose.
• Theory units – to facilitate uniform coverage of syllabus for paper setting.
• Suggested Readings – to recommend some standard books as reference material. This
does not unequivocally exclude other such reference material that may be
recommended according to the advancements and local requirements.
• A list of journals pertaining to the discipline is provided at the end which may be
useful as study material for 600-series courses as well as research topics.
• E-Resources - for quick update on specific topics/events pertaining to the subject.
• Broad research topics provided at the end would facilitate the advisors for appropriate
research directions to the PG students.
Minimum Credit Requirements
Subject Master’s programme Doctoral programme

Major 20 15
Minor 09 08
Supporting 05 05
Seminar 01 02
Research 20 45
Total Credits 55 75
Compulsory Non Credit Courses See relevant section
Major subject: The subject (department) in which the students takes admission
Minor subject: The subject closely related to students major subject (e.g., if the major
subject is Entomology, the appropriate minor subjects should be Plant Pathology &
Nematology).
Supporting subject: The subject not related to the major subject. It could be any
subject considered relevant for student’s research work.
Non-Credit Compulsory Courses: Please see the relevant section for details. Six
courses (PGS 501-PGS 506) are of general nature and are compulsory for Master’s
programme. Ph. D. students may be exempted from these courses if already studied
during Master’s degree.
8
DAIRY TECHNOLOGY
Course Structure - at a Glance
CODE COURSE TITLE CREDITS

MAJOR COURSES
DT 511* ADVANCED DAIRY PROCESSING 3+1
DT 512* ADVANCED FOOD PROCESSING 3+1
DT 513 RHEOLOGY OF DAIRY AND FOOD PRODUCTS 2+1
DT 514* DAIRY PROCESS BIOTECHNOLOGY 2+1
DT 515 TRADITIONAL AND VALUE-ADDED DAIRY 2+1
PRODUCTS
DT 521 MEMBRANE TECHNOLOGY IN DAIRY PROCESSING 2+1
DT 522 ADVANCED DAIRY AND FOOD PACKAGING 2+1
DT 523 ALTERNATIVE PROCESSES FOR THE DAIRY & FOOD 2+1
INDUSTRIES
DT 524* FUNCTIONAL FOODS AND NEW PRODUCT 3+1
DEVELOPMENT
DT 525 TECHNOLOGY OF FOOD EMULSIONS, FOAMS & GELS 2+1
DT 591 MASTER’S SEMINAR 1+0
DT 599 MASTER’S RESEARCH 20
DT 611 ADVANCES IN LIPID TECHNOLOGY 3+0
DT 612 ADVANCES IN PROTEIN TECHNOLOGY 3+0
DT 621 PRODUCT MONITORING AND PROCESS CONTROL 3+0
DT 622# R & D MANAGEMENT IN DAIRY INDUSTRY 3+0
DT 691 DOCTORAL SEMINAR I 1+0
DT 692 DOCTORAL SEMINAR II 1+0
DT 699 DOCTORAL RESEARCH 45
SUGGESTED SUPPORTING COURSES
ES 525 DAIRY BUSINESS MANAGEMENT 2+1
ES 529 STATISTICS IN INDUSTRIAL APPLICATIONS 3+1
CS 521 COMPUTER SOFTWARE 2+1
#
DCRT 524 RESEARCH TECHNIQUES 2+1
DC 523 CHEMICAL QUALITY ASSURANCE 2+1
DM 523 MICROBIAL QUALITY & SAFETY IN DAIRY INDUSTRY 2+2
DE 524 ENVIRONMENTAL ENGINEERING 2+0
* Compulsory for Master’s programme; # Cross-listed
NOTE : Doctoral students shall take a minimum of two 600-level courses
9
DAIRY TECHNOLOGY
Course Contents
DT 511 ADVANCED DAIRY PROCESSING 3+1

Objective
To provide in-depth knowledge in various unit operations and basic
concepts in dairy processing.
Theory
UNIT I
Use of bio-protective factors for preservation of raw milk: effects on
physicochemical, microbial and nutritional properties of milk and milk
products, present status of preservation of raw milk by chemical
preservatives; thermal processing for preservation.
UNIT II
Methods of determining lethality of thermal processing, UHT processed
milk products, their properties and prospects, types of UHT plants, aseptic
fillers, heat stability and deposit formation aspects, effect on milk quality;
techno- economic considerations; retort processing.
UNIT III
Principles and equipment for bactofugation and Bactotherm processes,
Microfluidization of milk: Principle, equipment, effects and applications,
Homogenization and their applications in dairy industry.
UNIT IV
Dehydration: advances in drying of milk and milk products; freeze
concentration, freeze dehydration: physicochemical changes during freeze
drying and industrial developments.
UNIT V
Water activity; sorption behaviour of foods, energy of binding water,
control of water activity of different milk products in relation to their
chemical; microbiological and textural properties; hurdle technology and its
application in development of shelf-stable and intermediate-moisture foods,
Use of carbonation in extending the shelf life of dairy products.
UNIT VI
Current trends in cleaning and sanitization of dairy equipment: biological;
detergents; Automation; Ultrasonic techniques in cleaning; bio-detergents,
development of sanitizers- heat; chemical; radiation, mechanism of fouling
and soil removal; Bio-films, assessing the effectiveness of cleaning and
sanitization of dairy products.
Practical
LP system for extension of keeping quality raw milk, determination of pH;
HCT profile of milk systems, measurement of thiocyanate in milk system;
determination of water activity and sorption isotherms of milk products;
determination of thermal load during retort processing of milk and milk
products; heat classification of milk powders; functional properties of
powders: porosity, interstitial air content, occluded air content, flowability;
determination of degree of browning-chemical/physical methods; freeze
drying of milk/milk products, and heat sensitive products. Homogenization
efficiency; cleaning efficiency in dairy equipment; visit to a UHT
Processing plant. Thermal process calculations.
10
Suggested Readings
Burton H. 1998. Ultra-high Temperature Processing of Milk and Milk
Products. Elsevier.
Fellow P. 1988. Food Processing Technology. Elliss Horwood Ltd.
Gould GW. 1995. New Methods of Food Preservation. Blackie.
IDF Bulletin 1981. New Monograph on UHT Milk. Document No. 133,
Intern. Dairy Fed., Brussels.
Smit G. 2003. Dairy Processing – Improving Quality. CRC-Woodhead
Publ.
Troller JA & Christian HB. 1978. Water Activity and Food, Food Science
and Technology. A Series of Monograph Academic Press, London.
Walstra P, Geurts TJ, Noomen A, Jellema A & Van Boekel MAJS. 1999.
Dairy Technology – Principles of Milk Properties and Processes.
Marcel Dekker.
DT 512 ADVANCED FOOD PROCESSING 3+1

Objective
To provide in-depth understanding of advances in theoretical and practical
aspects of food processing.
Theory
UNIT I
Status of food processing industry in India and abroad; prospects and
constraints in development of Indian food industry.
UNIT II
Post harvest management of fruits and vegetables, Harvesting indices,
Biochemical and physical changes during ripening of fruits & vegetables,
respiration and factors affecting it, role of ethylene in accelerated ripening,
post harvest treatments for extension of shelf-life of fresh produce,
Strategic interventions to minimize post harvest losses including vapour
heat treatment, wax coating, chemicals, etc.
UNIT III
Principles of chilling & refrigeration storage of foods, quality changes in
cold stored products, controlled and modified atmospheric storage.
Freezing of foods, principle and equipments for freezing, defects in frozen
foods, re-crystallization, freezing of fruits and vegetables, freeze
concentration of fruit juices.
UNIT IV
Application of heat energy to foods for preservation and processing,
concept of drying rate of foods, industrial drying processes of foods;
changes during drying, advanced drying processes (Freeze drying, infra red
drying and microwave drying), Canning of fruits & vegetables, unit
processes involved in canning, types of cans for thermal processing of
foods.
UNIT V
Basic principles involved in fermentation, Technological aspects of pickled
vegetables like sauerkraut, cucumbers, Technology of wine, beer and
distilled alcoholic beverages, defects in alcoholic beverages.
11
UNIT VI
Conversion of muscle into meat, rigor mortis, freezing and canning of
meat, curing & smoking of meat, fermented sausages, cooking of poultry,
utilization of milk ingredients in processed meat and poultry products.
UNIT VII
Advances in milling of rice (solvent extractive milling) and Turbo milling
of wheat. Bakery products; role of ingredients, Developments in
manufacturing processes for bakery products such as breads; biscuits; pizza
bases, cake etc; changes during processing of bakery products. Utilization
and importance of dairy ingredients in bakery products.
UNIT VIII
Definition, classification and technologies of fabricated and formulated
foods and their nutritional aspects. Imitation dairy products and dairy
analogues. Principle of extrusion processing, design and working of
extruder, classification, application in food and dairy processing. Food
additives, including stabilizers, emulsifiers, antioxidants, preservatives, etc.
for formulated foods.
UNIT IX
Important group of enzymes involved in food processing; Application of
enzymes in food processes like enzymes juice extraction, juice clarification,
in bread manufacture, meat tenderization, ice cream manufacture, de-
sugaring of egg, etc.
UNIT X
Newer concepts in food processing including organic foods, processing of
organic raw material, genetically modified foods.
Practical
MAP and its effect on shelf-life of fresh fruits and vegetables, Preparation
of squash, cordial, nectar and whey beverages, whey based soups,
Manufacture of bread, pizza base, biscuits and cake, Application of milk
ingredients in caramel, egg-less cake, mayonnaise, canning of fruits &
vegetables, manufacture of chicken soup, comminuted meat products,
enzymatic extraction and clarification of fruit juices, Preparation of
soymilk and tofu, Drying of fruits & vegetables, efficacy of blanching
treatment. Manufacture of sauerkraut/fermented vegetables.
Suggested Readings
Fellows PJ. 2000. Food Processing Technology: Principles and Practices.
2nd Ed. CRC-Woodhead Publ.
Fennema CR. 1975. Principles of Food Science. Part II. Physical Principles
of Food Preservation. Marcel Dekker.
Guy R. 2001. Extrusion Cooking: Technologies and Applications. CRC-
Woodhead Publ.
Honseney RC. 1986. Cereal Science and Technology. American
Association of Cereal Chemists, St. Paul, Minnesota.
Hui YH, Meunix-Goddick L, Hansen AS, Josephsen J, Nip WK, Stanfield
PS & Toldra F. 2004. Handbook of Food and Beverage
Fermentation. Marcel Decker.
Hui YH, Nip WK, Rogers RW & Young DA. 2001. Meat Science and
Application. Marcel Decker.
12
Norman W & Desrosier IN. 1987. The Technology of Food Preservation.
4th Ed. CBS Publ.
Penfield MP & Campbell AM. 1990. Experimental Food Science. 3rd Ed.
Academic Press.
Ramaswamy H & Marcotte M. 2006. Food Processing: Principle and
Application. Taylor & Francis.
Vangarde JS & Woodburn M. 1994. Food Preservation and Safety:
Principles and Safety. Iowa State University Press, Iowa.
DT 513 RHEOLOGY OF DAIRY & FOOD PRODUCTS 2+1

Objective
To explain the basics of food rheology, and to familiarize the students with
rheological instruments and their use in relation to dairy and food products.
Theory
UNIT I
Introduction to rheology of foods: Definition of ‘texture’, ‘rheology’ and
‘psychophysics’ – their structural basis; physical considerations in study of
foods; salient definitions –Stress tensor and different kinds of stresses.
UNIT II
Rheological classification of Fluid Foods : Shear-rate dependence and time
dependence of the flow-curve; Non-Newtonian fluids; thixotropy;
Mechanisms and relevant models for non-Newtonian flow; Effect of
temperature; Compositional factors affecting flow behaviour; Viscosity of
food dispersions – dilute and semi-dilute systems, concentration effects.
UNIT III
Comparative assessment of different types of Viscometers, and their Merits
and Limitations: Co-axial cylinders, Spindle- or Impeller-type viscometers,
Cone-plate viscometer, Capillary viscometers, Falling-sphere viscometer,
Vibratory viscometers, Extrusion viscometer, Orifice viscometer.
UNIT IV
Rheology of semi-solid and solid food ; Rheological characterization of
foods in terms of stress-strain relationship; Viscoelasticity; Transient tests -
Creep Compliance and Stress Relaxation; mechanical models for
viscoelastic foods: Maxwell, Kelvin, Burgers and generalized models and
their application; Dynamic measurement of viscoelasticity.
UNIT V
Large Deformations and failure in foods: Definitions of fracture, rupture
and other related phenomena; Texture Profile Analysis; Instrumental
measurements – Empirical and Fundamental methods; Rheometers and
Texture Analyzers; Measurement of Extensional viscosity; Acoustic
measurements on crunchy foods.
UNIT VI
Rheological and textural properties of selected dairy products:
Measurement modes and techniques; Effect of processing and additives
(stabilizers and emulsifiers) on food product rheology; Relationship
between instrumental and sensory data.
Practical
Study of different types of viscometers viz., co-axial cylinder viscometer,
spindle viscometer, falling-ball viscometer, extrusion viscometer, impeller
viscometer, orifice viscometer; Flow behaviour of fluid dairy products;
13
Thixotropy in ice-cream mix; force-deformation study in selected dairy
products using Texture Analyzer; Back extrusion; Effect of test conditions
on the texture profile parameters of cheese and similar products; stress
relaxation studies in solid foods; Use of Cone Penetrometer and FIRA-
NIRD extruder for measurement of butter texture; Use of a
Viscoamylograph for study on the gelatinization behaviour of starch/cereals
flours.
Suggested Readings
Barbosa-Canovas GV, Kokini JL, Ma L & Ibarz A. 1997. Rheology of
Semi-liquid foods. Adv. Food & Nutr. Res., 39:1-69.
DeMann JM, Voisey PW, Rasper VF & Stanley DW. 1976. Rheology and
Texture in Food Quality. AVI Publ.
NDRI. 1996. Sensory Evaluation and Rheology of Milk and Milk Products.
Lecture Compendium, CAS/DT Short Course, Aug. 22-Sept. 13,
1996. Dairy Technology Division, NDRl, Karnal.
Sherman P. 1970. Industrial Rheology. Academic Press.
DT 514 DAIRY PROCESS BIOTECHNOLOGY 2+1

Objective
To project the importance of biotechnology in dairy processing and impart
knowledge on all aspects of dairy process biotechnology in production and
preservation of dairy products employing the principles of biotechnology.
Theory
UNIT I
Definition of biotechnology; development and impact of biotechnology on
food and dairy industry.
UNIT II
Microbial rennet and recombinant chymosin, characteristics and application
in cheese making; exogenous free and microencapsulated enzymes,
immobilized enzymes-their application in accelerated ripening of cheese;
enzymatically modified cheeses (EMC) their utilization in various food
formulations.
UNIT 3
Technological requirements of modified micro-organisms for production of
cheese and fermented milk products; technological innovations in the
development of functional dairy foods with improved nutritional
therapeutic and pro-biotic attributes; physiologically active bio-peptides/
nutraceuticals; protein hydrolysates – their physicochemical, therapeutic
properties, production and application in food formulations; production of
bio-yoghurt, pro-biotic cheese and fermented milks; bifidus factors in
infant food formulations.
UNIT IV
Protein hydrolysates-production, their physicochemical, therapeutic
properties, de-bittering and application in food formulations; Enzymatic
hydrolysis of lactose for preparation of whey and UF-permeate beverages.
UNIT V
Microbial polysaccharides their properties and applications in foods,
production of alcoholic beverages and industrial products from starch;
14
whey and other by-products; bio-sweeteners-types properties and their
applications in dairy and food industry.
UNIT VI
Bio-preservatives- characteristics and their application in enhancing the
shelf life of dairy and food products.
Practical
Effect of exogenous enzymes on hydrolysis of protein and fat in culture
containing milk systems; to study the various factors affecting the
coagulation of milk by microbial rennets. Manufacture and evaluation of
pro-biotic cheese and fermented milks; determination of glycolysis,
proteolysis and lipolysis in cheese and fermented milk; enzymatic process
for manufacture of low lactose milk whey products; preparation of casein
hydrolysates; visit to a bio-processing unit.
Suggested Readings
Chirikjian JG. 1995. Biotechnology-Theory and Techniques. Jones &
Bartlett Publ.
Goldberg I. 1994. Functional Foods. Chapman & Hall.
Gutierrez-Lopez GF & Barbosa-Conovas GV. 2003. Food Science and
Food Biotechnology. CRC Publ.
Lee Byong H.1996. Fundamentals of Food Biotechnology. VCH
Publ.
Roger A. 1989. Food Biotechnology. Elsevier.
Wiseman A. 1988. Principles of Biotechnology. Surrey Univ. Press, New
York.
DT 515 TRADITIONAL AND VALUE ADDED PRODUCTS 2+1

Objective
To project the significance and status of traditional and value added dairy
products in Indian dairy industry.
Theory
UNIT I
Present status of traditional dairy products; globalization of traditional dairy
products; plans and policies of the Government and developmental
agencies.
UNIT II
Process schedule of heat-desiccated, coagulated and fermented traditional
dairy products; process improvement in production of milk sweets.
UNIT III
New products based on fruits, vegetables and cereals; application of mem-
brane technology; microwave heating for industrial production of
traditional dairy products.
UNIT IV
Advances in industrial production of ghee, flavour and texture simulation.
UNIT V
Techno-economic aspects for establishing commercial units for traditional
products.
UNIT VI
Convenience traditional dairy products; use of natural and permitted
synthetic preservatives and new packaging systems.
15
Practical
Microwave heating of traditional milk delicacies for shelf life extension;
application of membrane technology for improving the quality of
traditional products from cow and buffalo milk; preparation of feasibility
report for establishing commercial units for traditional products.
Suggested Readings
Achaya KT & Rangappa KS. 1975. Indian Dairy Products. 2nd Ed. Asia
Publ. House.
Aneja RP, Mathur BN, Chandhan RC & Banerjee AK. 2002. Technology of
Indian Milk Products. Dairy India Publ., Delhi.
De S. 1980. Outlines of Dairy Technology. Oxford Univ. Press Publ., New
Delhi.
Gould GW. 1995. New Methods of Food Preservation. Blackie.
NDRI. 1998. Advances in Traditional Dairy Products. Lecture
Compendium, Dairy Technology Division, NDRI, Karnal.
NDRI. 2006. Developments in Traditional Dairy Products. Lecture
Compendium, Dairy Technology Division, NDRI, Karnal.
DT 521 MEMBRANE TECHNOLOGY IN DAIRY PROCESSING 2+1

Objective
To explain the basics of membrane technology and its applications in dairy
processing.
Theory
UNIT I
Membrane techniques: classification and characteristics of filtration pro-
cesses; types of membranes commercially available; membrane hardware,
design of membrane plants, modeling of ultrafiltration (UF) processes,
mass transfer model, resistance model; membrane flouling-problem and
treatment, cleaning and sanitization of different types of membranes.
UNIT II
Factors affecting permeate flux during ultrafiltration and reverse osmosis of
milk and sweet and sour whey, energy requirements for processing of milk
and whey.
UNIT III
Applications of ultrafiltration, reverse osmosis, nanofiltration and
microfiltration in the dairy industry. Developments in the manufacture and
utilization of food and pharmaceutical grade lactose from UF permeate.
Preparation of special foods like low lactose powder and dairy whiteners
using UF retentate, whey protein concentrates, casein and coprecipitates.
UNIT IV
Demineralisation: Importance of demineralisation, different processes
available for demineralization: their principle, plant and operation.
UNIT V
Functional properties of whey proteins (WPC & WPI), casein, co-
precipitates and UF milk retentate and their modifications. Evaluation of
functional properties of proteins.
16
Practical
Study of the effect of types of milk, temperature of milk and trans-
membrane pressure on the permeate flux during ultrafiltration process;
performance of ultrafiltration membrane with respect to permeate flux and
volume concentration ratio during processing of acid and sweet whey;
study the effect of types of milk, temperature and applied pressure on the
permeate flux during the reverse osmosis process; nanofiltration of milk,
whey and permeate; microfiltration of skim milk and whey; preparation of
whey protein concentrate and its utilization in dairy products; measurement
of different functional properties of casein and whey protein: whipping
ability; water binding; emulsification properties; gelling; viscosity and
solubility.
Suggested Readings
Cheryan M.1998. Ultrafiltration and Microfiltration Handbook.
Technomic Publ. House.
IDF.1996. Advances in Membrane Technology for Better Dairy Products.
Bull. No: JI1.
Renner E & Abd El-Salam MH. 1991. Application of Ultrafiltration in the
Dairy Industry. Elsevier.
Sienkiewizc T & Tiedel CL. 1990. Whey and Whey Utilization. Verlag Th.
Mann Gelsenkirchen-Buer, Germany.
Zadow JG. 1994. Whey and Lactose Processing. Elsevier.
DT 522 ADVANCED DAIRY AND FOOD PACKAGING 2+1

Objective
To impart basic and advanced knowledge of dairy and food packaging.
Theory
UNIT I
Status of current packaging; types of packaging materials; criteria for
selection of proper packaging; testing of packaging materials.
UNIT II
Adhesives; graphics; coding, and labeling used in food packaging.
UNIT III
Protective packaging of foods; packaging of food products sensitive to
oxygen, light, moisture; active packaging; special problems in canned
foods.
UNIT IV
Packaging of dairy products; packaging of convenience foods, packaging of
fruits, vegetables, and fruit juices.
UNIT V
Packaging of fats and oils; packaging of spices; packaging of meat and
poultry: packaging of fish and other seafoods.
UNIT VI
Modified atmosphere packaging, controlled atmosphere packaging, shrink
and stretch packaging.
UNIT VII
Retort pouch technology, microwavable, biodegradable, and edible
packages.
17
UNIT VIII
Industrial packaging: unitizing, palletizing, containerising, distribution
systems for packaged foods including prevention of shock damage to
articles during transportation.
UNIT IX
Safety aspects of packaging materials; sources of toxic materials and
migration of toxins into food materials.
Practical
Testing of packaging materials for quality assurance like determination of
thickness, GSM, grease resistance, bursting strength, tearing resistance,
WVTR, puncture resistance; estimation of shelf life of vegetables and
seasonal fresh fruits; packaging of turmeric powder and ground red chilli
powder, vacuum packaging of dairy products.
Suggested Readings
Carol F, Steinhart M, Ellin D, Barbara A & Cochrane. 1995. Food Safety.
Marcel Dekker.
Coles R, McDowell D & Kirwan M.J. 2003. Food Packaging Technology.
Oxford Blackwell.
Frank A, Paine H & Paine Y. 1983. A Handbook of Food Packaging.
Leonard Hill.
Gordon L Robertson. 2006. Food Packaging: Principles and Practice. 2nd
Ed. CRC Press.
Malhlouthi M. 1994. Food Packaging and Preservation, Blackie.
Raija A. 2006. Novel Food Packaging. Woodland Publ. Co.
DT 523 ALTERNATIVE PROCESSES FOR DAIRY 2+1

AND FOOD INDUSTRY
Objective
To develop an understanding of the basic principles underlying the
novel/non-conventional food processing techniques, equipment
required, features and actual and potential applications.
Theory
UNIT I
Irradiation: sources and properties of ionizing radiation; mechanism of
interaction with microorganisms and food components microbial
inactivation in dairy and food products, chemical effects, packaging,
industrial irradiation systems, benefits and limitations; safety aspects,
national and international regulations.
UNIT II
High frequency heating: Principles of dielectric heating and factors
affecting it, design and working of microwave oven, continuous microwave
heating units, applications in dairy and food processing, microwavable
packaging safety aspects of microwaves, merits and demerits of dielectric
heating.
UNIT III
Infra-red heating: Interaction of infra-red (IR) radiation with penetration
properties, equipment; dairy and food application, advantages and
disadvantages of IR heating.
18
UNIT IV
Ohmic heating: Principle of electric resistance heating, design of an ohmic
heater, operational variables, power considerations, factors affecting
heating efficiency, merits and limitations, food applications and future
scope.
UNIT V
Ultrasonic treatment of food: Mechanism of ultrasound induced cell
damage, generation of ultrasound equipment, design of power ultrasonic
system, types of ultrasonic reactors, application of power ultrasound in
food processing, effects on food constituents, ultrasound in consideration
with other process alternatives - thermosonication, advantages and future
prospects.
UNIT VI
High hydrostatic pressure processing: Principle of microbial inactivation,
barotolerance of microorganisms, effect on food constituents, equipment,
dairy and food application, merit and demerits.
UNIT VII
Pulsed electric field processing: Description/ mechanism and factors
affecting microbial inactivation effects on food components; present status
and future scope for food applications.
Practical
Study of a microwave oven; Determination of power output of a
microwave oven; Temperature profile in a microwave oven cavity;
Microwave absorption by various food packaging materials; Heating
behaviour of water, milk, cream and other milk products – effect of
composition; Shelf-life extension of pasteurized milk employing
microwave heating; Effect of shape and size of water/milk container on
microwave heating; Cooking of ‘instant’ products in a microwave oven;
Drying of casein, ‘instant’ wheat, ‘instant’ rice, etc. in a microwave oven;
Miscellaneous food processing/heating applications of microwaves; Visit
to a commercial food irradiation facility.
Suggested Readings
Gaonkar KG. (Ed.). 1995. Food Processing: Recent Developments.
Elsevier.
Gould GW. (Ed.). 1995. New Methods of Food Preservation. Blackie.
NDRI. 2001. Advances in Preservation of Dairy and Food Products.
Lecture Compendium, Dairy Technology, NDRI, Karnal.
Povey MJW & Mason TJ. (Eds.). 1998. Ultrasound in Food Processing.
Blackie.
Thorne S. 1991. Food Irradiation. Elsevier.
DT 524 FUNCTIONAL FOODS AND NEW PRODUCT 3+1

DEVELOPMENT
Objective
To impart the knowledge of functional ingredients, nutraceuticals and their
utilization in development of new food products including health foods,
functional foods and specialty foods.
19
Theory
UNIT I
Definition, classes of functional foods, status of functional foods in world
and India. Concept of new product development, classes of nutraceuticals
and functional foods. Safety; marketing strategy and consumer response;
economic analysis and costing of novel foods, recent advances in different
categories and type of dairy product.
UNIT II
Nutritional status and dietary requirement of different target group and
deficiency diseases, in special reference to micronutrients. Dietary and
therapeutic significance of dairy nutrients, bioactive components in dairy
products like lactose, whey proteins, milk minerals, CLA, fermented milks
etc.
UNIT III
Food fortification, techniques for fortifying dairy foods with minerals and
vitamins, High protein foods prospective nutraceuticals for fortification of
dairy foods. Nutritional significance of dietary fibers, classes of dietary
fibers, fortification techniques for fibers in dairy foods.
UNIT IV
Infant nutrition and dietary formulations for meeting normal and special
needs of infants, current status of infant foods, additives for infant foods.
Foods for aged persons, design consideration, ingredients for geriatric
foods.
UNIT V
Technological aspects of reduced calorie foods, alternatives for calorie
reduction, low calorie sweeteners, bulking agents and their application, fat
replacers and their utilization in low calorie dairy foods.
UNIT VI
Nutritional and health significance of sodium in foods, Alternatives for
sodium in foods, techniques for reducing sodium in processed dairy foods.
Bio-flavours and flavour enhancers.
UNIT VII
Sports foods, ingredients for sports foods, dairy components in sports
foods, sports drinks, design consideration, ergogenic aids in sports
nutrition.
UNIT VIII
Herbs, various classes of herbs, their therapeutic potential and application
in foods with special reference to dairy products like functional drinks,
herbal ghee etc.
UNIT IX
Prebiotic substances and their utilization in functional foods, symbiotic
foods, technological aspects and recent development in probiotics,
prebiotics and synbiotics.
UNIT X
Definition and various classes of phytochemicals, their role in CVD,
Cancer and immune system enhancer, utilization in functional foods,
phytosterol, phytoestrogens, glucosinolates, organosulphur compounds,
flavonoids, carotenoids, etc.
UNIT XI
Special foods/nutrients for CVD, cancer, IBD, diabetics, persons suffering
20
with milk allergy and lactose intolerance with special emphasis on dairy
nutrients and foods.
Practical
1. Determination of total fiber, neutral detergent fiber in foods
2. Manufacture of fiber enriched milk beverage
3. Manufacture of low calorie burfi/ice cream
4. Preparation of flavoured milk using artificial sweetener and its
estimation
5. Determination of antioxidant activity of food/food components
6. Determination of bioavailability of nutrients
7. Development of malted milk food and weaning food
8. Determination of β-galactosidase activity and application of lactases for
lactose free dairy products
9. Determination of prebiotic potential of certain plant/milk components
and their application in synbiotics dairy foods
10. Preparation of sports beverage, herbal dairy drinks
11. Preparation of high protein products
Suggested Readings
Chadwick R. 2003. Functional Foods. Springer.
Desai BB. 2000. Handbook of Nutrition and Diet. Marcel Dekker.
Gibson G & William C. 2000. Functional Foods. CRC Press.
Goldberg I. (Ed.). 1994. Functional Foods. Chapman & Hall.
Haberstroh CE. 1991. Fat and Cholesterol Reduced Foods. Gulf Publ. Co.
Mazza G. 1998. Functional Foods. Biochemical and Processing Aspects.
Technomic Publ. Co.
Mitchell H. 2006. Sweeteners and Sugar Alternatives in Food Technology.
Oxford’s Blackwell Publ.
Mitchell JR & Ledward DA. 1986. Functional Properties of Food
Macromolecules. Elsevier.
Mudambi SR & Rajagopla MV. 1981. Fundamentals of Foods and
Nutrition. Wiley Eastern.
Nabors Lyn O’Brien 2001. Alternative Sweeteners. Marcel Dekker.
Pomeranz Y. 1991. Functional Properties of Food Components. Academic
Press.
Sadler MJ & Saltmarch M. 1998. Functional Foods: The Consumer, The
Products and the Evidence. Royal Society of London.
Saltmarch M & Butriss J. (Ed.). 2000. Functional Foods II: Claims and
Evidence. Royal Society of Chemistry, London.
Schmidl MK & Labuza TP. 2000. Essentials of Functional Foods. Aspen
Publ.
Shi J, Mazza G & Maguer M Le. 2002. Functional Foods: Biochemical and
Processing Aspects. CRC Press.
Smith J. 1993. Technology of Reduced Additives. Blackie.
Watson RR. 2003. Functional Foods and Neutraceuticals in Cancer
Prevention. Iowa State Press, Iowa.
21
DT 525 TECHNOLOGY OF FOOD EMULSIONS, FOAMS AND GELS 2+1
Objective
To impart basic knowledge regarding food dispersion systems, their
formation, behaviour, and factors affecting their stability.
Theory
UNIT I
Food dispersions, their characteristics and factors affecting food
dispersions.
UNIT II
Food emulsions; emulsifiers and their functions in foods; the HLB concept
in food emulsifiers; emulsion formation and stability; polymers and
surfactants.
UNIT III
Milk foams and their applications, structure of foams, egg foams and uses,
foam formation and stability.
UNIT IV
Theory of gel formation; pectic substances and jellies; fruit pectin gels;
milk jellies.
UNIT V
Structure of dairy foods representing emulsions, foams and gels; physical
structure of fat rich, concentrated, fermented, coagulated and dried
products.
UNIT VI
Techniques for evaluation of structure for food emulsions, foams and gels.
Practical
Determination of the rate of formation and stability of emulsions;
Emulsifying properties of milk proteins and other food ingredients;
Properties of different types of emulsifiers and their role in food emulsions;
Examination of foam formation and determination of foam stability; Milk
proteins and other food ingredients in food foams; foaming in dairy
systems; Studies on gel formation and gel properties; Food gels – Gelatin-
based, pectin-based, etc.; Properties of various gelling agents for foods.
Suggested Readings
Blanshard JMV & Lillford P. 1987. Food Structure and Behaviour.
Academic Press.
Haseuhuetti GL.1977. Food Emulsifiers and their Application. Chapman &
Hall.
McClement DJ.1999. Food Emulsions - Principles, Practice and
Techniques. CRC Press.
Srinivas D & Alain P.1977. Food Proteins and their Applications. Marcel
Dekker.
DT 611 ADVANCES IN LIPID TECHNOLOGY 3+0

Objective
To explain the physicochemical and nutritional characteristics of fats and
oils, and their processing and utilization for food purposes.
Theory
UNIT I
Current Trends in the fats & oil Industry in India and abroad: Sources and
utilization of animal, vegetable and marine fats/oils - An overview.
22
UNIT II
Basic chemistry structure aspects of fats and oils in general, and milk-fat in
particular, in relation to their processing, properties, utilization and
significance in human diet.
UNIT III
The changing concepts in the role of edible fats and oils in human nutrition
and health: PUFA, MUFA, CLA, Omega fatty acids, Trans fatty acids,
Phytosterol, etc.
UNIT IV
Sources and classification of commercial edible fats and oils : Innovations
in the production and processing of oils and fats from different sources, e.g.
animal, plant, marine and microbial lipids for utilization in the dairy and
food products; Non-conventional fats/oils for edible purpose – rice bran oil,
microbial lipids.
UNIT V
Advances in refining including degumming, bleaching and deodorization
oils and fats – Existing technologies and new developments - application of
membrane techniques; Winterization.
UNIT VI
Physico-chemical properties of oils & fats; Polymorphism, crystallization
kinetics; New antioxidants.
UNIT VII
Modification of fats and oils: Physical modification – Fractionation;
Chemical modification – Hydrogenation and Interesterification;
enzymatic/microbial modification of fats/oils; Cholesterol reducing
treatments.
UNIT VIII
Applications of fats and oils: Margarine and low-fat table spreads; Bakery
and confectionery fats; Coatings; Shortenings; Salad dressings; Technology
of cooking oils, salad oils and oil based dressings; Frying process and
systems; Changes in fats and oils during frying; Snack foods - Processing
systems; Modified fats and oils for use in bakery and confectionery
products, shortenings and spreads.
UNIT IX
Fat replacers, technological developments in low calories spreads and other
fat-based products.
UNIT X
Advances in technologies for production of plasticisers, emulsifiers and
protective coatings.
Suggested Readings
Cambie RC. 1989. Fats for the Future. International Union of Pure and
Applied Chemistry. Ellis Horwood.
Gunstone FD & Padley FB. (Eds.).1997. Lipid Technologies and
Applications. Marcel Dekker.
Hamilton RJ.1995. Developments in Oils and Fats. Blackie.
Hoffman G.1989. Chemistry and Technology of Edible Oils and Fats and
their High-Fat Products. Academic Press.
Hui YH. (Ed.).1996. Wiley Bailey's Industrial Oil and Fat Products. Vols.
I-IV. Interscience Publ., John Wiley & Sons.
Kamal BS & Kakuda Y.1994. Technological Advances in Improved and
23
Alternative Sources of Lipids. Blackie.
Karleskind A. (Ed.). 1996. Oils and Fats Manual. Vol. II. Properties,
Production and Applications. Lavoisier Tec-Doc Publ.
Khan R.1993. Low-Calories Foods and Food Ingredients. Blackie.
DT 612 ADVANCES IN PROTEIN TECHNOLOGY 3+0

Objective
To explain the characteristics of food proteins and to familiarize students
with their implications in processing, their interactions in food systems and
their nutritional role.
Theory
UNIT I
Characteristics of proteins from plant, animal and microbial origins.
UNIT II
Denaturation of proteins: Effect of processing parameters on denaturation.
Effect of denaturation on the physicochemical and biological properties of
proteins in food systems.
UNIT III
Protein interactions with food constituents: protein-protein interactions.
Protein-lipid interactions, protein-polysaccharide interactions, and protein-
ion interactions. Significance of protein interactions: formation and
stabilization of casein micelle, stability of concentrated milk products, and
role of protein in food structure.
UNIT IV
Protein Nutrition: Recent concepts in protein nutrition in man: Enzyme
development and protein digestion. Effect of processing on nutritive value
of proteins. Mass and institutional feeding programs: Amino acid
fortification of foods and concepts in protein supplementation and
complementation.
UNIT V
Protein hydrolysates-- Production and processing; De-bittering of protein
products; Bioactive peptides: their production and properties.
UNIT VI
Recent Technologies: Augmentation of world resources for protein foods:
protein from plants, animals and microorganisms.
UNIT VII
Textured vegetable proteins and spun fibre technology: Extrusion cooking-
selection of ingredients and formulation, control of operational parameters,
microstructure of extrusion cooked foods.
Suggested Readings
Encyclopaedia of Food Science, Food Technology and Nutrition. 1993.
Vols. I-VII, Academic Press.
Fennema OR. 1985. Food Chemistry. Marcer Dekker.
Fox PF. 1983. Development in Dairy Chemistry. Vol. II. Applied Science
Publ.
Fox PF. 1992. Advanced Dairy Chemistry. Vol I. Proteins. Elsevier.
Macrac R, Robinson RK & Sadler MJ. 1993. IDF Special Issue 9303.
Walstra P & Jenness R. 1984. Dairy Chemistry and Physics. John Wiley &
Sons.
24
DT 621 PRODUCT MONITORING AND PROCESS CONTROL 3+0
Objective
To develop the understanding of the concept of monitoring and
optimization of food quality/characteristics and familiarize the students
with the techniques involved.
Theory
UNIT I
The concept of Product-Process Monitoring in dairy and food industries:
Definition of ‘quality’; Optimization paradigm; Quality-prediction model
based on quality kinetics and process state equations – Simulation
modeling. Process/Product Optimization: Optimization procedures – Search
methods, Response surface, Differentiation & Programming methods;
Neural Networks; Optimization software.
UNIT II
Process Control: Objectives; Control loop – Loop elements and their
functions; Modes of process control; Control techniques; control
equipment.
UNIT III
Real-time Instrumentation : Sensors; their classification based on
Proximity, working principle; examples of applications in process control;
Requirements of on-line sensors; Biosensors – Construction, types,
working principles, applications, merits and limitations; Time-temperature
indicators – partial-history & full-history indicators; Commercial devices;
Applications and limitations; E-Nose & E-Tongue – Simulation of natural
organs; Components & their functions; Applications.
UNIT IV
Flavour analysis: Flavour bioassays – Gas Chromatography-Olfactometry
techniques; Isolation, Separation and detection/Identification of flavour
compounds – GC-MS, LC-MS, NMR, FTIR; analysis of chiral compounds.
UNIT V
Formation of flavour compounds in milk and milk products during heat
processing (including UHT processing, caramelization and extrusion
cooking), fermentation and ripening (cultured. products and cheese flavour,
with special reference to bitteness) and storage (Maillard browning);
Aroma losses/retention during the drying process (in relation to milk
powder, cheese powder and dry cultured products); Industrial processes for
extraction of desirable and undesirable volatile components from fresh
and/or stored products by supercritical fluid (SCF) technique.
UNIT VI
Monitoring of Food Structure: (a) Application of Thermal Analysis (DTA
and DSC vis-a-vis dilatometry) and Pulse Nuclear Magnetic Resonance
(PNMR) spectroscopy in determination of solid-fat content (SFC) of butter
in relation to various processing and storage aspects; Glass transitions in
dairy products; Starch gelatinization.
UNIT VII
Monitoring of Food Structure: (b) Elucidation of crystal characteristics of
milk fat in ghee and other fat-rich products by means of X-ray
Crystallography with reference to the impact of cooling and
storage/handling conditions on the crystal nature and product texture;
25
Process-induced changes in sub-microscopic particulate properties of milk
products; structure-texture relationship.
UNIT VIII
Monitoring of Food Structure: (c) Influence of heat processing and freezing
treatments on protein denaturation and other conformational as well as
aggregation-disaggregation phenomena as monitored through
Spectropolarimetry, Circular Dichroism and related techniques.
UNIT IX
Monitoring of Food Structure: (d) Particle-size analysis: Image analysis;
Dynamic light scattering; Laser diffraction; Sieving, and other techniques.
UNIT X
Emerging Spectroscopic techniques in assessment of foods : Raman
Spectroscopy and Electron Spin Spectroscopy – Working principles and
applications - Monitoring of irradiated foods, detection of lipid auto-
oxidation, etc.; Microwave & NIR absorption/reflection methods for
Compositional analyses – Automated milk analysers; Proximate principles
in cheese and milk powder.
UNIT XI
Ultrasound in product monitoring: Speed- and Attenuation-based
measurements of liquid levels, density, mass flow, etc.
UNIT XII
Monitoring of Chemical Contaminants: Heavy metal quantification by
Atomic Absorption Spectrophotometer; Quantification of Agrochemicals
by HPLC; Spectrofluorimetric determination of mycotoxins; Detection and
quantification of Drug Residues.
UNIT XIII
Colour Characterization : Colour and appearance (gloss and translucence)
monitoring through visual colorimeter, tri-stimulus colorimeters and
reflectance spectrophotometer, CIE, Hunter-Lab, Munsel and other systems
of three-dimensional expression of colour; Colour-based Sorting of foods;
Computer Vision – Principles, applications and Benefits.
UNIT XIV
Objective Assessment of Subjective food-quality characteristics - Pitfalls
and Promises.
Suggested Readings
Acree TE & Teranishi R. 1993. Flavour Science: Sensible Principles and
Techniques. Amer. Chem. Soc., Washington.
Bartlett PN, Elliott JM & Gardner JW. 1997. Electronic Noses and their
Application in the Food Industry. Food Technology: 51 (12) 44-48.
Davenel A. 1996. On-line Control and Problems with Sensors. In: Quality
Control for Foods and Agricultural Products. (J.L. Mutton, Ed.).
VCH Publ., London.
Kress-Rogers E & Brimelow CJB. (Eds.). 2001. Instrumentation and
Sensors for the Food Industry. CRC Press, Woodhead Publ. Ltd.
Mann CMD & Jones AA. I994. Shelf-life Evaluation of Foods. Blackie.
Peleg M & Bagley EB. 1983. Physical Properties of Foods. AVI Publ.
Schartel BJ & Firstenberg ER. 1988. Biosensors in the Food Industry:
Present and Future. J. Food Protect. 51(10): 811-820.
26
DT 622 RESEARCH AND DEVELOPMENT MANAGEMENT 3+0
IN DAIRY INDUSTRY
Objective
To provide in-depth knowledge to research scholar in selection and
management of research project in the area of new product development
and in patenting and transfer of technology processes.
Theory
UNIT I
Current Status of R&D Efforts in Dairy Processing in India and abroad.
UNIT II
Resource Management: Management of financial and human resources in
dairy Industry: a) Structure and design of Research and Development
orgnisation; b) Analysis of organization behaviour – Transactional analysis;
and c) Personnel management – Typology analysis, individual and the
organization, team building, human behaviour at work, motivation.
UNIT III
Management of R&D functions: a) Criterion for the selection of R&D
projects; Technology Development Process and b) Techniques for
monitoring R & D functions.
UNIT IV
Patenting Laws; Indian Patenting Act/International Protocols for
technology transfer; Transfer of technology from Lab to Plant, HACCP,
GMP/GHP practices in dairy processing. ISO14001, Total Quality
Management (TQM), Six-Sigma concept.
UNIT V
Project proposal writing for research funding, Development of feasibility
and technical report for dairy plant establishment, evaluation and report
writing of projects.
Suggested Readings
Encyclopedia of Food Technology and Nutrition. 1993. Academic Press.
General Information on Patents. The Patent Office, Kolkatta.
IPR Bulletins (TIFAC) (www.tifac.org.in)
NAARM. 1990. Agricultural Research Management. National Academy of
Agricultural Research Management, Hyderabad.
27
DAIRY TECHNOLOGY
List of Journals
• American Journal of Clinical Nutrition

• Australian Journal of Dairy Technology
• Bioprocess and Biosystems Engineering
• Comprehensive Reviews in Food Science and Food Safety
• CRC Critical Reviews in Food Science and Nutrition
• Food Engineering
• Food Research International
• Food Science & Technology (LWT)
• Food Science and Technology International.
• Food Technology
• Indian Dairyman
• Indian Food Packer
• Indian Journal of Dairy Science
• International Dairy Journal
• International Journal of Dairy Technology
• International Journal of Food Properties
• International Journal of Food Science and Technology
• Journal of American Oil Chemists’ Society
• Journal of Applied Microbiology.
• Journal of Biotechnology
• Journal of Chemical Technology and Biotechnology
• Journal of Dairy Research
• Journal of Dairy Science
• Journal of Food Biotechnology
• Journal of Food Engineering
• Journal of Food Processing and Preservation
• Journal of Food Protection
• Journal of Food Science
• Journal of Food Science and Biotechnology
• Journal of Food Science and Technology
• Journal of Food Technology
• Journal of Human Nutrition and Dietetics
• Journal of Lipid Research
• Journal of Texture Studies
• Packaging Abstracts
• Packaging India
• Trends in Food Science and Technology
28
Suggested Broad Topics for Master’s and Doctoral Research
• Application of UF retentates in value added products and health foods.

• Application of reverse osmosis in dairy industry.
• Application of microfiltration in dairy industry.
• Application of nanofiltration in dairy industry.
• Application of modified microorganisms in the manufacture of improved quality
dairy products.
• Bio-processing of whey for production of alcoholic beverages.
• Characteristics of newly developed microbial rennet in relation to curd forming
behavior of ripened and unripened cheeses
• Crystallization behaviour of milk-fat and its impact on texture of butter and ghee.
• Development of kinetic data on physicochemical changes in different dairy/food
products during processing and storage.
• Development of low-cost quality food formulations with protein supplementation.
• Development of newer fermented milk products with enhanced health attributes.
• Development of newer variety of cheeses from microbial/recombinant rennet using
cow and buffalo milk systems.
• Development of pro-biotic cheese and fermented foods.
• Development of improved infant food formulations.
• Development of casein/ whey protein hydrolysates with enhanced functionality for
food application.
• Development of fortified foods meant for community nutrition
• Development of indigenous detergents for cleaning and sanitization of membranes.
• Development of mechanized systems for the production of heat desiccated milk
sweets/desserts, such as rabri, basundi, kheer/payasam, kalakand, milk cake, etc.
• Dielectric/microwave heating characteristics of milk and milk products – effect of
composition and degree of concentration.
• Effects of processing variables on the textural properties of certain indigenous milk
products.
• Enhancement of flavor development in cheeses.
• Enhancement of shelf life of food products through packaging.
• Effects of ultrasonic treatment of milk products on their properties and microbial
inactivation.
• Effect of bactofugation keeping quality of milk under Indian conditions.
• Extension of shelf life of dairy products by applying Hurdle Technology.
• Effect of season on the compositional profile and physicochemical properties of
milk-fat.
• Flavour characteristics of fats from cows, buffaloes, goats and sheep.
• Foaming properties of milk as influenced by its constituents
• Foam formation in cream-- Factors affecting the extent and stability of foam.
• Investigation on quality improvement of dairy foods using novel ingredients.
29
• Incorporation of fruits for value addition and improvement of nutritional value of
traditional milk products.
• Incorporation of cereals for value addition and improvement of nutritional value of
traditional milk products.
• Infra-red heating behaviour of milk and milk products.
• Investigation of prebiotic potential of milk molecules and their utilization in
development of synbiotic foods
• Isolation and purification of natural antibacterial substances of milk for
preservation of milk products
• Low-temperature irradiation of dairy products.
• Process modification of manufacture and storage of ghee to improve granulation
and flavour of ghee.
• Preservation of milk by LP system: study of safety aspects through clinical trials.
• Preservation of dairy foods by employing bio-preservatives.
• Process development for herbal dairy products
• Physicochemical properties of buffalo, goat and sheep milk-fats.
• Rheological and textural characterization of certain traditional dairy foods.
• Relationship between instrumental measurements of dairy/food product
characteristics and their sensory assessment.
• Shelf life extension of traditional milk sweets through use of bio preservatives.
• Structure-texture relationships in selected dairy products.
• Studies on extrusion cooking of high protein foods.
• Studies on UHT processing of concentrated milk.
• Studies on the effectiveness of bio-detergents.
• Studies on process development for health foods utilizing milk/milk by-products
• Studies on development of low calorie dairy foods
• Study of moisture sorption isotherms of traditional dairy products and milk sweets
• Study of functional properties of UF retentates.
• Study of fouling behaviour during UHT processing of buffalo milk.
• Technology development for manufacture of specialty foods for
aged/sports/diseased persons
• Technology for development of milk-cereal fermented foods
• Technology for production of bioactive milk components and their application in
functional foods
• Use of antimicrobial packaging material for shelf life extension of traditional and
value added dairy products
• Utilization of dairy by-products in formulated foods
• Utilization of dairy byproducts in production of value added products employing
biotechnology.
• Viscoelastic behaviour of buffalo-milk cheeses and other semi-solid products
• Viscometric characterization of flowable buffalo-milk products
30
DAIRY MICROBIOLOGY

DM 511 MICROBIAL MORPHOLOGY AND TAXONOMY 2+1
DM 512* MICROBIAL PHYSIOLOGY 2+1
DM 513 METHODS IN MICROBIOLOGY 2+2
DM 514 ENVIRONMENTAL MICROBIOLOGY 2+1
DM 515* MICROBIOLOGY OF PROCESSED DAIRY FOODS 3+1
DM 516 APPLICATION OF BIOTECHNOLOGY IN DAIRY INDUSTRY 2+1
DM 521* DAIRY STARTER CULTURES 2+1
DM 522 MICROBIAL GENETICS 2+1
DM 523* MICROBIAL QUALITY AND SAFETY IN DAIRY INDUSTRY 2+2
DM 524 FEED AND RUMEN MICROBIOLOGY 2+1
DM 525 PROBIOTICS AND FERMENTED DAIRY PRODUCTS 2+1
DM 526 MICROBIAL FERMENTATION AND TECHNOLOGY 2+1
DM 591 MASTER’S SEMINAR 1+0
DM 599 MASTER’S RESEARCH 20
DM 611 MICROBIAL DIVERSITY AND PHYSIOLOGY 3+0
DM 612 ADVANCES IN MICROBIAL GENETICS 3+0
DM 621 ADVANCES IN DAIRY AND FOOD MICROBIOLOGY 3+0
DM 622 MICROBIOLOGY OF FOOD-BORNE PATHOGENS 3+0
DM 691 DOCTORAL SEMINAR I 1+0
DM 692 DOCTORAL SEMINAR II 1+0
DM 699 DOCTORAL RESEARCH 45

* Compulsory for Master’s programme.
31
DAIRY MICROBIOLOGY
Course Contents
DM 511 MICROBIAL MORPHOLOGY AND TAXONOMY 2+1

Objective
To educate about the taxonomy and morphological features of the various
microorganisms, viz., bacteria, fungi and viruses.
Theory
UNIT I
Evolution of life on earth, history and diversity of microorganism
UNIT II
Principles of classification and taxonomy of Eubacteria (Bacteria and
Archaea): Major characteristics used in taxonomy: Cultural, Morphological,
Biochemical, Physiological, Genetic and Molecular Characteristics; Numerical
Taxonomy (Taxometrics) and Chemotaxonomy. Assessing Microbial
Phylogeny: Chronometers. Phylogenetic trees, r-RNA, DNA and proteins as
indicators of phylogeny.
UNIT III
Cell ultra-structure (prokaryotes and eukaryotes) cell wall: structure, chemical
composition, synthesis and inhibition, cell membrane, cytoplasmic inclusions,
cytoskeleton, cell appendages, capsule, flagella, pili, sporulation structure of
endospore, composition and function of spore constituents, induction and
germination
UNIT IV
Fungi: Distribution, Importance and recent Classification, Study of Yeasts and
Moulds in Dairy Foods
UNIT V
History, Development and Scope of Virology. Classification and
Nomenclature, General characteristics of Viruses (Acellular Organization and
Viral Genome), Viral Reproduction, Brief account of Viroids and Prions.
Practical
• Simple and differential staining; gram, spore, acid-fast staining, cell wall,
flagella, nucleoids, capsule, and inclusion/storage bodies
• Preparation of bacterial protoplasts and spheroplasts
• Measuring dimensions of microorganisms (bacteria) using micrometry,
• Study of morphology of fungi,
• Detection and enumeration of bacteriophages in Cheese whey
• Application of computer software in bacterial identification
• Electron microscopic observation of Ultra-structure of Microbial
Cell(Demonstration)
Suggested Readings
Alberts B, Johnson A, Lewis I, Raff M, Roberts K & Walter P. 1998.
Essentials of Cell Biology. Garland Publ.
Alexopoulos C. 2008. Introductory Mycology. Wiley Publ.
Black JG. 2005. Microbiology: Principles and Explorations. 6th Ed. John
Wiley & Sons.
Dimmock NJ & Primrose SB.1994. Introduction to Modern Virology. 4th Ed.,
Blackwell.
Holt JG, Krieg NR, Sneath PHA, Staley JT & Williams ST. 1997. Bergey's
32
Manual of Determinative Bacteriology. 9th Ed. Williams & Wilkins.
Krejer van-Rij NJW.1998. The Yeasts: A Taxonomic Study. 4th Ed. Elsevier.
Madigan MT, Martinko JM & Parker J. 2005. Brock Biology of
Microorganisms. 11th Ed. Prentice Hall. London.
Prescott LM, Harley JP & Klein DA. 2002. Microbiology. 5thth Ed. McGraw
Hill.
Salyers AA & Whitt DD. 2001. Microbiology - Diversity, Disease and the
Environment. Fitzgerald Science Press.
Tortora GJ, Berdell RF & Christine L Case. 2006. Microbiology: An
Introduction. 9th Ed. Benjamin Cummings.
DM 512 MICROBIAL PHYSIOLOGY 2+1

Objective
To familiarize the student with the various aspects of growth and
energy generating activities of bacteria for the betterment of human
life.
Theory
UNIT I
Bacterial growth: growth phases and kinetics, synchronous, continuous, and
associative growth, Diauxic phenomenon; factors affecting bacterial
growth; growth measurement.
UNIT II
Effect of environment on the growth of bacteria: temperature, air, osmotic
pressure, pH, hydrostatic pressure, surface tension, metals, electromagnetic
and other waves, sonics, various chemicals; their application in dairy
industry; mechanisms of action of antimicrobials such as antimicrobials.
UNIT III
Bacterial nutrition; nutrient media; nutritional groups of bacteria; role of
growth factors; active and passive transport.
UNIT IV
Electron transport chain: Electron transport chain; fermentation, respiration
and photosynthesis
Practical
Measurement of bacterial growth by direct methods (cell number, SPC,
DMC) and indirect methods (turbidometric methods, MPN, cell mass.
Preparation of growth curve; determination of generation time.
Determination of cell activity; carbohydrate fermentation; acid
production/pH alteration; starch, lipid, casein and gelatin hydrolysis.
Effect of different factors viz., physical (temperature, pH, osmotic
pressure, surface tension), chemical (dyes, antibiotics, phenol) and
nutritional (amino acid supplements, vitamin supplements, protein
hydrolysates, casamino acids) on bacterial growth.
Suggested Reading
Caldwell DR. 1995. Microbial Physiology and Metabolism. WMC Brown
Publ.
Koch L. 1995. Bacterial Growth and Form. Chapman & Hall.
Moat AG, Foster JW & Spector MP. 2002. Microbial Physiology. 4th Ed.
Wiley-Liss.
Nicoloi S Panikov. 1995. Microbial Growth Kinetics. Chapman & Hall.
33
Todar K. 2002. Todar's online Textbook of Bacteriology. University of
Wisconsin-Madison.
http://www.sciencedirect.com/science/bookseries/00652911
DM 513 METHODS IN MICROBIOLOGY 2+2

Objective
To impart knowledge and skills related to microbiological analytical
systems in microbiology and related sciences
Theory
UNIT I
Microscopy: principles, design and functions of bright field, dark field,
phase contrast and fluorescence microscope; principle, design and
application of transmission and scanning electron microscopes for the study
of sub-cellular organization and microstructure of dairy foods
UNIT II
Techniques for protein analysis and other molecular separation:
electrophoresis, chromatography, ultracentrifugation; Enzyme analyses and
substrate determination methods
UNIT III
Molecular Biology Techniques for nucleic acid analysis: amplification,
investigation of mutations and gene expression
UNIT IV
Aerobic and Anaerobic culturing techniques for isolation of obligate and
facultative organisms
UNIT V
Use of animal models in toxicity studies
Practical
• Familiarization with the construction and design of a compound
microscope; use of light microscope accessories; microscopic analysis
of different types of bacteria by bright field and dark field; phase
contrast and fluorescence microscopes
• Disruption of bacterial cells by ultra-sonification
• Demonstration of chromatographic techniques
• Demonstration of aerobic and anaerobic culturing techniques
• Demonstration of use of animal models in toxicity studies,
• Demonstration of PCR technique as a tool for identification and
characterization of microorganism
Suggested Readings
Gerhardt P, Murray RGE, Wood WA & Kreig NR. 1994. Methods for
General and Molecular Biology. ASM Press.
Hartley WG. 1993. The Light Microscope; It's Use and Development.
Science Publishing Co.
Herman B. 1998. Fluorescence Microscopy. Bios Scientific Publ.
Kaufman PB, Wu W, Kim D & Cseke LJ.1995. Molecular and Cellular
Methods in Biology and Medicine. CRC Press.
Singer S. 2001. Experiments in Applied Microbiology. Academic Press.
Wilson K & Walker J. 2008. Principles and Techniques of Biochemistry
and Molecular Biology. 6th Ed. Cambridge University Press.
34
DM 514 ENVIRONMENTAL MICROBIOLOGY 2+1
Objective
To understand the fundamentals of environmental microbiology for overall
effects of microorganisms in combating the pollution in the environment.
Theory
UNIT I
Microorganisms as components of the environment and their role in
nutrient cycling; extreme environments and microbial ecology.
UNIT II
Microbes in aquatic and terrestrial environment; aero-microbiology;
microorganisms as indicators of environment pollution; bio-organic
pollution.
UNIT III
Microbial toxicants and pollutants and their biodegradation; organic
pollutants and their degradation; biodegradation of plastics and polymers.
UNIT IV
Biofouling and biofilms; bioremediation and metabolic engineering; water
pollution and control.
UNIT V
Biological treatments of food industry wastes; Issues concerning release of
genetically engineered microorganisms in environment; environment laws.
Practical
Determination of BOD in industrial wastes; Determination of composite
micro-flora of selected environmental samples; Detection of low levels of
xenobiotics, microbial toxins and residual antibiotics in environmental
samples; Isolation of bacteria capable of degrading organic and microbial
pollutants from environmental samples; Isolation and characterization of
bio-indicators from environmental samples; Visit to a sewage and sludge
treatment plant.
Suggested Readings
Bitton G. 1994. Waste-water Microbiology. John Wiley & Sons.
Hurst CJ, Crawford RL, Garland JL, Lipson DA & Mills AL. 2007. Manual
of Environmental Microbiology. 3rd Ed. ASM Press.
Maier RM, Pepper IL & Gerba CP. 2000. Environmental Microbiology.
Elsevier.
Mitchell R. 1995. Introduction to Environmental Microbiology. 8th Ed.
Prentice-Hall of India.
Pepper IL & Gerba CP. 2004. Environmental Microbiology: A Laboratory
Manual. 2nd Ed. Elsevier.
DM 515 MICROBIOLOGY OF PROCESSED DAIRY FOODS 3+1

Objective
To understand microbiology of processed foods, significance of different
food microorganisms, their control and other related aspects.
Theory
UNIT I
Developments in food microbiology, microbial ecology of processed food;
factors that influence microbes in foods.
35
UNIT II
Thermal processes for shelf stable-products, low temperature food
preservation, current concepts in irradiation technology; Biopreservation.
UNIT III
Stress induced injury to microorganisms, enumerations of stressed cells,
predictive modeling for food spoilage; industrial strategies for ensuring safe
foods.
UNIT IV
New methods for controlling spoilage of foods; active packaging and
antimicrobial packaging (AMP), modified atmosphere packaging (MAP)
and shelf life of processed foods, Intermediate moisture foods and Hurdle
concept.
UNIT V
New prospects and problem in fermented foods, Nutraceuticals and
Bioactive foods, Genetically Modified (GM) foods and their acceptance.
Practical
• Productions of antimicrobial substances.
• Application of bacteriocins, Biopreservation of foods
• Application of hurdle concepts for enhanced shelf stability of processed
foods
• Induction of bacterial cell injury and recovery of injured cells
• Effect of MAP on quality and shelf life of processed foods.
Suggested Readings
Doyle MP, Benchat LR & Monteville TJ. 1997. Food Microbiology:
Fundamentals and Frontiers. ASM Press.
Ray B. 2003. Fundamental Food Microbiology. CRC Press.
Robinson RK. 1998. Developments in Food Microbiology. Vols. I-III.
Elseveir.
Susan KH & Theodore PL. 1986. Biotechnology in Food Processing.
Noyes Publ.
DM 516 APPLICATION OF BIOTECHNOLOGY IN DAIRY INDUSTRY 2+1

Objective
To impart knowledge in the application of Biotechnology in Dairy / Food
Industry to the students of Dairying at Master’s level
Theory
UNIT I
History and Development of Biotechnology; Status of Biotechnology
Industries in India to meet the demands of Dairy and Food Industry
UNIT II
Genetic improvement of lactic starters to enhance their technological
functions for industrial applications e.g. acid, flavour, EPS, probiotic
functions, Metabolic engineering of lactic acid bacteria, Production of
recombinant dairy / food enzymes / proteins e.g. Chymosin, lactoferrin,
lysozyme, lipases, proteases, immunoglobulins etc. Detection of GMOs
and GM foods and their safety from public health point of view
UNIT III
Dairy based Functional foods/Health foods and Nutraceuticals. Value
addition in dairy products through fortification/supplementation with
bioactive components and probiotic cultures, Nutrigenomics
36
UNIT IV
Application of molecular tools, biosensors, etc. for detection of food borne
and spoilage pathogens
UNIT V
Molecular tools for studying Biodiversity; Regulatory standards for GMOs
and GM foods.
Practical
• Plasmid isolation from E. coli
• Agarose gel electrophoresis
• Transformation of E. coli with plasmid (Ampr)
• Growth of Starter cultures on MRS for ‘lac’ marker
• Induction of ‘lac’ mutation using UV rays or ethidium bromide
• PCR assays for identification of LAB and food-pathogen detection
Suggested Readings
Bains W. 1993. Biotechnology from A to Z. Oxford Univ. Press, Oxford.
Crueger W & Crueger A. 1984. Biotechnology: A Textbook of Industrial
Microbiology. Science Tech.
Joshi VK & Pandey A. (Ed.). 1999. Biotechnology. Food Fermentation. (2
Vol. set). Education Publ.
Knorr D. 1982. Food Biotechnology. Marcel Dekker.
DM 521 DAIRY STARTER CULTURES 2+1

Objective
To familiarize the students with the starter organisms, their metabolism and
genetics; different types of starters, propagation, preservation and
applications of starters.
Theory
UNIT I
Introduction and annual utilization of starter cultures; History and
taxonomy of starter cultures; Classification of starter organisms: The genus
Lactococcus; The genus Leuconostoc; The genus Streptococcus; The genus
Pediococcus, The genus Lactobacillus.
UNIT II
Adjunct starter organisms; The genus Bifidobacterium; The genus
Enterococcus; The genus Propionibacterium; The genus Brevibacterium.
Miscellaneous microorganisms: Molds and yeasts.
UNIT III
Metabolism of starter organisms: biochemical characterization of lactic
acid bacteria; carbohydrate, citrate and protein metabolism of starter
cultures.
UNIT IV
Genetics of starter cultures: plasmids and plasmid instability; industrially
significant genes and systems; genetic modification of lactic acid bacteria
through transduction; conjugation; protoplast transformation;
electroporation and chromosomal integration, transposons and insertion
sequences.
UNIT V
Starter types: single, mixed and multiple strain starter cultures; propagation
and preservation of starter cultures; commercial starter preparations:
37
concentrated and super-concentrated starters;
UNIT VI
Growth inhibition of lactic acid bacteria by antibiotics, bacteriocins;
immunoglobulins and bacteriophage: sources, types and characteristics of
phages associated with starters, phage control during starter handling and
growth, mechanisms of phage resistance in LAB.
UNIT VII
Probiotic cultures, health and nutritional benefits, requirements for ability
to survive and grow in the intestine, control of intestinal infections. Role of
starter cultures in cheese making and ripening of different cheese varieties.
Practical
• Isolation of lactococcal cultures from fermented milks.
• Examination of purity and activity of starter cultures.
• Preservation of starter cultures by freeze drying and other methods.
• Preparation of concentrated starters and quality evaluation.
• Inhibition of starters by antibiotic residues and other inhibitors.
• Plasmid profiles of some lactococcal cultures.
• Identification of lactic starters by molecular biology techniques
(demonstration).
• Conjugal transfer of plasmids in lactococci (demonstration).
• Production of bacteriocins by LAB.
Suggested Readings
Cogan TM & Accolas JP. 1995. Dairy Starter Cultures. VCH Publ.
Law BA. 1997. Microbiology and Biochemistry of Cheese and Fermented
Milks. 2nd Ed. Blackie.
Marth EM & Steele JL.1998. Applied Dairy Microbiology. Marcel Dekker.
Robinson RK. 1998. Developments in Food Microbiology. Vol. IV.
Elsevier.
Salminen S & Wright AV. 1998. Lactic Acid Bacteria. Marcel Dekker.
Wood BJ & Warner PJ. (Eds.). 2003. Genetics of Lactic Acid Bacteria.
Springer- Verlag.
DM 522 MICROBIAL GENETICS 2+1

Objective
To understand the fundamentals of structure, functions and synthesis of
macromolecules and their genetic manipulation.
Theory
UNIT I
Macromolecules: DNA, RNA, their structure, types, organization, function
and their properties, DNA replication, damage and repair.
UNIT II
Gene Expression and its regulation in Prokaryotes and Eukaryotes:
Transcription; Genetic Code Translation and post translational
modifications; Negative and positive regulation of gene expression; Operon
Models (lac and trp operon).
UNIT III
Mutagenesis, mutation and mutants; Lamda phage and its gene
organization.
38
UNIT IV
Plasmids and their properties, transposable elements; Bacterial
Recombination- Transformation, Conjugation and Transduction.
UNIT V
Fundamental aspects of genetic engineering - Vectors, Restriction enzymes,
gene cloning, gene banks, expression of cloned genes. Applications of
Genetic Engineering- Restriction Mapping, site directed mutagenesis,
polymerase chain reaction and application of genetic engineering in dairy
and food industry.
Practical
• Isolation and quantitative estimation of chromosomal DNA from E.coli
and Lactobacillus by mini prep method.
• Isolation of plasmid DNA from E.coli and Lactobacillus by miniprep
method.
• Isolation of Eukaryotic (yeast) chromosomal DNA
• Calcium chloride induced transformation of E.coli hosts with plasmids
• Induction of random mutation in E.coli and Lactobacillus by UV
radiations and chemical mutagens
• Curing of plasmids with chemical mutagens (Ethidium Bromide) and
elevated temperature
• Preparation of chloroplast and their regeneration and uptake in bacteria
• Use of restriction enzymes cleavage and ligation of DNA fragments
• P.C.R. based detection of microorganisms.
Suggested Readings
Friedberg EC & Walker GC. 1995. DNA Repair and Mutagenesis. Jones &
Bartlett Publ.
Friefelder D. 1987. Microbial Genetics. Jones & Bartlett Publ.
Lewin B. 1980. Gene Expression. Vols. I, II. John Wiley & Sons.
Old EW & Primrose SB. 1981. Principles of Gene Manipulation.
Blackwell.
Strickberger MW. 1985. Genetics. Macmillan Publ.
Synder L & Champness W. 2003. Molecular Genetics of Bacteria. ASM
Publ.
Uldis N Streips & Ronald E Yasbin (Eds.). 2004. Modern Microbial
Genetics. John Wiley & Sons.
Watson JD. 2003. Molecular Biology of Genes. W.A. Benjamin.
Wilson WH. 1985. Genetic Recombination. Benjamin/Cummings Publ.
DM 523 MICROBIAL QUALITY AND SAFETY 2+2

IN THE DAIRY INDUSTRY
Objective
To impart current knowledge pertaining to quality and safety functions in
dairy processing unit
Theory
UNIT I
Principles of Quality and safety systems including QMS, HACCP,SAFE,
GMP, SSOP, personnel hygiene and food handling in dairy industry
39
UNIT II
Establishment of Microbiological standards, guidelines and specification
for foods; microbiological criteria; two and three class attributes plan for
sampling
UNIT III
Rapid detection methods including commercial detection kits; automatic
detection techniques for hygiene indicators, pathogenic organisms,
antibiotic/ pesticide residues and aflatoxin M1 in dairy foods
UNIT IV
Public health concern associated with milk and milk products; type of
microbial spoilage, defects and control measures
UNIT V
Trends in food borne diseases and implications; method of diseases
transmission; principles of safety in a food microbiological laboratory
Practical
Conventional and rapid techniques for Microbiological quality evaluation
of raw and pasteurized milk and other dairy products for hygiene indicators
i.e. aerobic plate count, Staph aureus, coliform, enterococci,
enterobacteriaceaea counts, yeast and molds count ; detection of common
dairy pathogens and other contaminants from milk and milk products i.e E.
coli , B. cereus , salmonella, Listeria, antibiotic residues and aflatoxin M1;
shelf life studies of dairy products; effect of storage condition and
packaging material on microflora of dairy foods.
Suggested Readings
Marshall RT. 1992. Standard Methods for the Examination of Dairy
Products. 16th Ed. APHA.
Morgan MRA, Smith CJ & William PA. 1992. Food Safety and Quality
Assurance. Elsevier.
Mortimore S & Wallace C. 1994. HACCP; A Practical Approach.
Chapman & Hall.
NRC. 1985. An Evaluation of Role of Microbiological Criteria for Foods
and Food Ingredients. Subcommittee on Microbiological Criteria
Committee on Food Protection. National Academic Press,
Washington.
Patel PD. 1994. Rapid Analysis Techniques in Food Microbiology. Blackie.
Robinson RK. 1990. Dairy Microbiology. Vols. I, II. Applied Science Publ.
Yadav JS, Grover S & Batish VK. 1993. Comprehensive Dairy
Microbiology. Metropolitan Publ.
DM 524 FEED AND RUMEN MICROBIOLOGY 2+1

Objective
To understand the basics of microbiology of feed and rumen ecosystem for
its outcome on dairy production, processing and management.
Theory
UNIT I
Biofertilizers; microbial inoculants for fodder crops; legume-rhizobium
symbiosis; production and application of biofertilizers.
UNIT II
Silage fermentation: microbial and chemical changes; use of additives and
inoculants; losses during ensiling.
40
UNIT III
Bioconversion of crop-residues by solid state fermentation; single-cell
protein production; advantages and disadvantages.
UNIT IV
Rumen microbial ecosystem; numbers, types, characteristics and functions
of rumen bacteria, fungi and protozoa.
UNIT V
Methods for isolation, enumeration and cultivation of rumen microbes;
their role in rumen metabolism and fiber degradation; exploitation of
fibrolytic microorganisms as direct-fed microbials/ microbial feed
additives.
Practical
Isolation of rhizobium bacteria from fodder legume plants; preparation of
rhizobium inoculant for fodder leguminous crops; estimation of moisture,
NH3-N and pH in silage; enumeration of silage microorganisms; estimation
of lactic, acetic and butyric acids in silage; sampling technique of rumen
liquor and direct microscopic count of bacteria and protozoa; anaerobic
cultivation techniques for bacterial and fungal counts in rumen liquor; use
of anaerobic jar, roll-tube technique, estimation of hydrolytic enzyme
activities of rumen liquor/ rumen microbes; SCP production from cellulose
in submerged fermentation; bioconversion of straw by solid state
fermentation.
Suggested Readings
Hobson PN & Stewart CS. 1997. The Rumen Microbial Ecosystem. 2nd Ed.
Chapman & Hall.
Makkar HPS & McSweeney CS. 2005. Methods in Gut Microbial Ecology
for Ruminants. Springer.
Pandey A. 1994. Solid-state Fermentation. Wiley Eastern.
Wallace RJ & Chesson A. 1995. Biotechnology in Animal Feeds and
Animal Feeding. VCH Publ.
Woolford MK. 1984. The Silage Fermentation. Marcel and Dekker.
DM 525 PROBIOTICS AND FERMENTED DAIRY PRODUCTS 2+1

Objective
To impart knowledge on basic and applied aspects of probiotics and
fermented dairy products.
Theory
UNIT I
Introduction and history of probioitcs and fermented dairy products.
UNIT II
Classification of fermented dairy products. Consumption statistics and
scope for fermented dairy products.
UNIT III
Microbiology of yoghurt and related products. Microbiology of acidophilus
products, dahi, misti dahi, bhapa dahi, lassi, cultured butter milk, shrikhand,
kefir, koumiss, yakult, villi, bifidus milk products, kishk, and milk based
products containing probiotic cultures.
UNIT IV
Factors influencing the quality and storage stability of fermented milks.
Methods used in their manufacture, evaluation and quality control.
41
UNIT V
Nutritional and therapeutic value of these products in human diet. Use of
fermented milks in diet and cancer control.
Practical
• Manufacture of different fermented milks and their microbiological and
chemical analysis.
• Study of shelf life of fermented milks using different methods of
preservation.
• Microbiological assay of vitamins or amino acids in fermented milk.
Suggested Readings
Cogan TM & Accolas JP. 1995. Dairy Starter Cultures. VCH Publ.
Milks. 2nd Ed. Blackie.
Marth EM & Steele JL.1998. Applied Dairy Microbiology. Marcel Dekker.
Robinson RK. 1998. Developments in Food Microbiology. Vol. IV.
Elsevier.
Salminen S & Wright AV. 1998. Lactic Acid Bacteria. Marcel Dekker.
Wood BJ & Warner PJ. (Eds.). 2003. Genetics of Lactic Acid Bacteria.
Springer-Verlag.
DM 526 MICROBIAL FERMENTATION TECHNOLOGY 2+1

Objective
To disseminate recent information on basic and applied aspects of
fermentation technology and its industrial application to the students along
with hands on training.
Theory
UNIT I
Introduction to fermentation. Historical perspective, Fermentation as a
means for enhancing shelf life of foods and pickles.
UNIT II
Rate of microbial growth and death, Fermentation kinetics, mass transfer
diffusion, membrane transport, dialysis, nutrient uptake.
UNIT III
Fermenter/ Bioreactor design, operation, measurement and control in
fermentation. Aeration and Agitation in fermentation; Oxygen requirement,
Adsorption coefficient bubble aeration, mechanical agitation, correlation
between mass transfer coefficients and operating variables.
UNIT IV
Types of fermentation, submerged / solid state, Batch / continuous
fermentation. In situ sterilization, Scale up in fermentation, Product
recovery, Role of Immobilization, Downstream processing for
Recombinant proteins and bio-assays
UNIT V
Industrial production of Lactic acid, Penicillin, Betagalactosidase, amino
acids, vitamins, ethanol.
Practical
• Follow up of bacterial growth in batch culture.
• Different methods of microbial cultivation
42
• Fermenter operation and measurement.
• Production of starters, baker yeast culture, alcohol, alcoholic beverages.
Suggested Readings
Crueger W & Crueger A. 1984. Biotechnology: A Textbook of Industrial
Microbiology. Science Tech.
Joshi VK & Pandey A Ed. 1999. Biotechnology. Food Fermentation. (2
Vol. set). Education Publ.
Stanburry PP & Whitker A. 1984. Principles of Fermentation Technology.
Pergamon Press.
Steinkraus KH. 1983. Handbook of Indigenous Fermented Foods. Marcel
Dekker.
DM 611 MICROBIAL DIVERSITY AND PHYSIOLOGY 3+0

Objective
To understand the advances in microbial diversity and physiology for its
interface with all other branches of microbiology.
Theory
UNIT I
Bacterial growth: cell division, phases of bacterial growth, factors affecting
microbial growth, kinetics of growth, continuous culture system, diauxic
and synchronous growth, advances in growth measurement.
UNIT II
Prokaryotic cellular structures, biosynthesis of bacterial cell-wall,
enterobacterial common antigens, role of membrane in regulation of cell-
wall and DNA synthesis, physiology and genetic aspects of sporulation.
UNIT III
Autotrophy, nutritional classification, chemolithotrophic electron transport
systems: hydrogen oxidizing bacteria, sulfate reducing hydrogen bacteria,
methanogenic hydrogen utilization, nitrifying bacteria, sulfur oxidizing
bacteria, carbon monoxide oxidizing organisms, economic importance of
autotrophs.
UNIT IV
Membrane transport systems: types of transport: ion transport, iron
transport, simple diffusion, facilitated diffusion, active transport,
mechanosensitive channel, ATP binding cassette transporter family,
chemiosmotic driven transport, phosphotransferase system.
UNIT V
Environmental selection and microbial stress response: osmotic stress,
aerobic to anaerobic transitions, oxidative stress, pH stress and acid
tolerance, thermal stress and nutritional stress, extremophiles.
Suggested Readings
Caldwell RD.1999. Microbial Physiology and Metabolism. WCB Publ.
Kim BH & Gadd G. 2008. Bacterial Physiology and Metabolism.
Cambridge Univ. Press.
Moat AG, Foster JW & Spector MP. 2004. Microbial Physiology. 4th Ed.
John Wiley & Sons.
Rhodes PM & Stanbury PF. 2008. Applied Microbial Physiology: A
Practical Approach. Oxford Univ. Press.
43
White D. 2006. The Physiology and Biochemistry of Prokaryotes. 3rd Ed.
Oxford Univ. Press.
DM 612 ADVANCES IN MICROBIAL GENETICS 3+0

Objective
To familiarize the students with basic concepts of Microbial Genetics and
impart them knowledge in advancements of Microbial Genetics and
Genetic Engineering
Theory
UNIT I
Essentials of Microbial Genetics – Introduction, Historical perspective and
principles; Nucleic Acids: Structure and Function of DNA and RNA; DNA
Replication –Recent models; Genetic Code
UNIT II
Mutations – Spontaneous and Induced mutations; Types of mutations;
Mutagenic agents - Physical and Chemical; Molecular basis of
Mutagenesis; DNA Damage and Repair – Molecular Mechanisms; Site
Directed Mutagenesis – Methods of directed mutagenesis, mechanism and
applications
UNIT III
Gene Expression – Transcription, Translation and Regulation of Gene
Expression - Operon models (Lac, Gal and Trp)
UNIT IV
Plasmids – Structure and replication; Transposable elements – IS and Tn
elements; molecular mechanism of transposition; Genetic Recombination:
Transformation, Transduction and Conjugation; Recombination methods as
a tool for Gene mapping
UNIT V
Genetic Engineering / rDNA – Principles of recombinant DNA technology;
Restriction Enzymes – Types, Mode of action and application as a tool for
gene manipulation, Vectors – Cloning and expression vectors; PCR
cloning, Microarray technology, Gene Silencing and Gene knock out
Suggested Readings
Bates AD & Maxwell A. 2006. DNA Topology. Univ. Press, Oxford.
Bergman NH. 2007. Comparative Genomics. Vols. I, II. Humana Press.
Hardin C, Edwards J, Ricll A & Prescotti D. 2001. Cloning, Gene
Expression and Purification. Univ. Press, Oxford.
Dale J & Schantz MV. 2002. From Gene to Genome: Concepts and
Application of DNA Technology. New John Wiley & Sons.
Dyson MR & Durocher Y. 2007. Expression Systems. Scion Publ.
Hartl D, Jones L & Elizabeth W. 2000. Genetic Analysis of Genes and
Genomes. Jones Bartkett Publ.
Keuzer H & Massey A. 2001. Recombinant DNA and Biotechnology. 2nd
Ed.. ASM Press.
Lewin B. 2004. Gene VIII. Pearson Edu.
Malacinski GM. 2003. Essentials of Molecular Biology. 4th Ed. Jones &
Bartlette Publ.
Streips UN & Yasbin RE. 2002. Modern Microbial Genetics. 2nd Ed. John
Wiley & Sons.
44
Synder L & Champness W. 2003. Molecular Genetics of Bacteria. 2nd Ed.
ASM Publ.
Watson JD. 2003. Molecular Biology of Gene. 5th Ed. The Benjamin/
Cummings.
Winnacker EL. 2003. From Gene to Clones. Panima Publ.
DM 621 ADVANCES IN DAIRY AND FOOD MICROBIOLOGY 3+0

Objective
To study and understand the current trends and recent concepts related
microbiology of dairy and other foods products.
Theory
UNIT I
Lactic acid bacteria and food fermentations important metabolic pathways
of microorganisms; current status of metabolism of starters cultures; current
trends in lactic starter for industrial applications, novel starter preservation
techniques.
UNIT II
Modern concepts in cheese ripening; Bacteriology and starter rotations,
improving starter cultures for food fermentation by genetic manipulation,
recombination technology.
UNIT III
Bacteriocins of lactic acid bacteria, structure, function transport and mode
of action; Application of bacteriocins in foods biopreservation.
UNIT IV
Current trends in food safety; newly emerging pathogens; Ecology and
survival strategy of pathogens in foods. Novel technology in control of
food based pathogens. Concepts in food toxicology; food borne toxins,
current concepts in food quality and safety management
Suggested Readings
Adams MR, Moss MO. 2002. Food Microbiology. 2nd Ed. Panima Publ.
Batt CA, Patel PA & Robinson RK. 1999. Encyclopedia of Food
Microbiology. (Set 1-3). Academic Press.
DeVuyst & Vandamme. 2000. Bacteriocins of Lactic Acid Bacteria.
Blackie.
Jay JM, Lossener MJ & Golden DA. 2005. Modern Food Microbiology.
Springer- Verlag.
Marth EH & Steele JL. 2001. Applied Dairy Microbiology. Marcel Dekker.
Ray B. 2003. Fundamental Food Microbiology. CRC Press.
Robinson RK. 2002. Dairy Microbiology Handbook: The Microbiology of
Milk and Milk Products. 3rd Ed. John Wiley & Sons.
Salminen S, Atte VW & Ouwehand A. 2004. Lactic Acid Bacteria. Marcel
Dekker.
Wood B & Warner PJ. 2003. Genetics of Lactic Acid Bacteria. Springer-
Verlag.
DM 622 MICROBIOLOGY OF FOOD-BORNE PATHOGENS 3+0

Objective
Upon satisfactory completion of this course, students should have
developed knowledge, understanding and application of Foodborne
pathogens at an advanced level.
45
Theory
UNIT I
Foodborne Pathogens: Host Invasion; Pathogenesis; Molecular approaches
for detection, identification, typing and analysis of foodborne pathogens;
Biosensor –based detection of foodborne pathogens.
UNIT II
Staphylococcal Gastroenteritis: Incidence in Foods, Staphylococcal
Enterotoxins: Types and Incidence, The Gastroenteritis Syndrome,
Prevention of Staphylococcal and other Food-Poisoning Syndromes
UNIT III
Botulism: Analysis of Dairy Products for C. botulinum and Botulinal
Toxin, Clinical Manifestations, Outbreaks, Prevention; Bacillus Cereus
food poisoning: Analysis of Dairy Products for B. cereus and Toxin,
clinical manifestation, outbreaks, prevention.
UNIT IV
Brucellosis: Clinical Manifestations, Outbreaks, Prevention; Foodborne
Listeriosis, Clinical Manifestations, Thermal Properties, Virulence
Properties, Animal Models and Infectious Dose, Listeriosis Syndromes,
Prevention.
UNIT V
Salmonellosis: Isolation and Detection Methods, Clinical Manifestations,
Outbreaks, Prevention. Foodborne Gastroenteritis Caused by Escherichia
coli, Enteropathogenic E. coli, Enterohemorrhagic E. coli O157:H7,
Clinical Manifestations, Outbreaks, Prevention. Campylobacteriosis:
Isolation and Identification, Clinical manifestations, Outbreaks, Prevention.
Yersiniosis: Isolation and Identification, Clinical manifestations,
Outbreaks, Prevention
Suggested Readings
Doyle MP, Beuchat LR & Montville TJ. 2001. Food Microbiology:
Fundamentals and Frontiers. 2nd Ed. ASM Press.
Food and Drug Administration. Food-borne Pathogenic Microorganisms
and Natural Toxins Handbook: The Bad Bug Book.
http://www.cfsan.fda.gov/cgi-bin/printall.cgi
Fratamico PM, Bhunia AK & Smith JL. 2005. Food-borne Pathogens:
Microbiology and Molecular Biology. Caister Academic Press.
Hocking AD. et al. 2003. Food-borne Microorganisms of Public Health
Significance. 6th Ed. AIFST (NSW Branch) Food Microbiology
Group, Sydney.
Jay JM. 2000. Modern Food Microbiology. 6th Ed. Aspen Publ.
Labbe RG & Garcia S. 2001. Guide to Food-borne Pathogens. John Wiley
& Sons.
Marth EH & Steele JM. 2001. Applied Dairy Microbiology. 2nd Ed. Marcel
Dekker.
Robinson RK, Batt CA & Patel PD. 2000. Encyclopedia of Food
Microbiology. Vols.I-III. Academic Press.
46
DAIRY MICROBIOLOGY
List of Journals
• Advances in Microbial Physiology
• Antimicrobial Agents and Chemotherapy
• Antonie van Leeuwenhoek
• Animal Feed Science and Technology
• Annals of Clinical Biochemistry
• Advances in Microbial Physiology
• Advances in Applied Microbiology
• Annals of Microbiology
• Annual Review of Microbiology
• Applied and Environmental Microbiology
• Applied and Environmental Microbiology
• Applied Biochemistry and Microbiology
• Applied Microbiology and Biotechnology
• Archives of Animal Nutrition
• Archives of Environmental Health
• Archives of Microbiology
• Asian-Australasian Journal of Animal Sciences
• Bioscience, Biotechnology and Biochemistry
• British Journal of Nutrition
• British Medical Journal.
• Cellular Microbiology
• Clinica Chimica Acta
• Clinical Chemistry.
• Clinical Chemistry and Laboratory Medicine
• Clinical Microbiology
• Critical Reviews in Environmental Science and Technology
• Critical Reviews in Microbiology
• Current Advances in Clinical Chemistry
• Current Contents
• Current Genetics
• Current Microbiology
• Dairy Science and Technology (Le Lait)
• Ecotoxicology and Environmental Safety
• Environment International
• Environmental Microbiology
• Environmental Monitoring and Assessment
• Environmental Research
• Environmental Science and Pollution Research
• Enzyme and Microbial Technology
• Eukaryotic Cell
• European Journal of Clinical Microbiology and Infectious Diseases
• FEMS Microbiology Ecology
• FEMS Microbiology Letters
• FEMS Microbiology Reviews
47
• Food Microbiology
• Food Microbiology and food safety journals
• Food Research International
• Foodborne Pathogens and Disease
• Fungal Genetics and Biology
• Gene
• Genome
• Indian Journal of Animal Nutrition
• Indian Journal of Animal Sciences
• Indian Journal of Microbiology
• Indian Journal of Veterinary Science
• Indian Journal of Dairy and Biosciences
• International Journal of Environment and Pollution
• International Journal of Food Microbiology
• International Journal of Food Science and Nutrition
• International Journal of General and Molecular Microbiology
• International Journal of Probiotics and Prebiotics
• International Journal of Systematic and Evolutionary Microbiology
• Investigation
• Journal of Animal Science
• Journal of Animal and Feed Sciences
• Journal of Applied Animal Research
• Journal of Applied Microbiology
• Journal of Bacteriology
• Journal of Basic Microbiology
• Journal of Biological Chemistry
• Journal of Biotechnology
• Journal of Chromatography A
• Journal of Experimental Animal Science
• Journal of Food Protection Letters in Applied Microbiology
• Journal of Food safety
• Journal of General and Applied Microbiology
• Journal in Genetics and Genomics
• Journal of Industrial Microbiology and Biotechnology
• Journal of Microbial Food Safety Standards.
• Journal of Microscopy
• Journal of Molecular Microbiology and Biotechnology
• Journal of Rapid Methods and Automation in Microbiology
• Journal of Virology
• Microbial Pathogenesis
• Microbial Ecology in Health and Disease
48
• Microbiological Research
• Microbiology: Bacteriology, Mycology, Parasitology and Virology
• Microbiology and Molecular Biology Reviews
• Molecular and Cellular Biology
• Molecular and Cell Biology
• Molecular Biology
• Molecular Genetics, Microbiology ,virology
• Nature
• Nature Biotechnology
• New England Journal of Medicine
• Plasmid
• PNAS
• Process Biochemistry
• Scandinavian Journal of Clinical and Laboratory
• Science
• Science of the Total Environment
• Symposium on Microbiological Food Safety Management 2007
• Systematic and Applied Microbiology
• The Journal of Biological chemistry
• The Lancet
• Trends in Food Science and Technology
• Trends in Microbiology
• Veterinary Microbiology
• Veterinary Research
• World Journal of Dairy and Food Sciences
• World Journal of Microbiology and Biotechnology
49
• Bioprospecting of dairy foods and related environment for identification,
characterization and classification of prevailing microbiota
• Study of ultra-structure of spore forming and non-spore forming dairy/food
microorganisms with the help of electron microscopy
• Detection of phages in dairy and food environment
• Study of mode of action of antibacterial substances on cellular organelles.
• Study of biofilms formation in milk handling and dairy processing environment.
• Alternative methods of microbial quantification
• Development of indicators and biosensors from microbial metabolites
• Energy metabolism
• Formulation of novel pharmaceuticals and neutraceuticals
• Microbial stress metabolism and ecosystem
• Harnessing the potential of microbial growth in environmental depollution
• Use of microorganisms in conversion of food wastes in preparation of newer foods
• Fermentation Studies for cultivation of lactic acid bacteria
• Study of probiotic organisms by growing them under anaerobic conditions and
their identification by PCR method
• Study of production of functional biomolecules by lactic acid bacteria
• Animal studies of functional attributes of dairy organisms
• Detection of pathogens by molecular biological methods.
• Role of extremophiles in microbial ecology and industry.
• Air micro-flora as spoilage and infectious agents in dairy industry.
• Microorganisms as indicators of environment pollution.
• Bio-organic pollution and its control measures.
• Biodegradation of pollutants and packaging of food materials in the environment.
• Biofilms in dairy industry.
• Bioremediation of food industry wastes and metabolic engineering.
• Novel bacteriocins of lactic acid bacteria
• Anti microbial packaging and MAP of foods
• Stress induced injury: mechanism and application in hurdle technology
• Genetic modification of food through the use of food grade vectors
• Rapid method for detection and identification of food pathogens
• Genetic manipulation of Lactic starter cultures
• Improving functionality of probiotics through metabolic engineering
• Recombinant proteins/enzymes for application in dairy industry
• Molecular diagnostics in dairy/food industry
• Biodiversity of LAB
• Bioactive peptides and Nutraceuticals
• GMO and GM foods and safety regulations.
• Regulation of metabolism for lactic acid and flavour production
• Genotypic heterogeneity and diversity of microorganisms in fermented dairy
foods.
• Phage resistance in lactic acid bacteria
• Defined strain cultures for indigenous fermented milks
• Plasmid borne genes, chromosomal integration and technological properties of
LAB.
50
• Study of Plasmid linked properties of dairy cultures.
• Transformation of gene of interest in the bacterial hosts
• PCR based identification of pathogens.
• PCR based identification of dairy cultures and probiotic cultures
• Genetic modification of dairy cultures by rDNA technology
• Principles of food safety control programme on HACCP, standard sanitary
operating procedures (SSOP) and GMP for dairy industry
• A process approach to quality management system
• Definitions, purpose and need for microbiological criteria. General principles for
the establishment and application of microbiological criteria for foods.
Consideration of sampling associated with a criterion
• Detection and enumeration of indicator organisms in dairy foods
• Detection and enumeration of conventional and emerging pathogenic organisms
and other contaminants in dairy foods
• Principles of bio-safety in establishment of pathogen testing laboratory in food
industry
• Trends in food borne diseases and implications; method of diseases transmission;
principles of safety in a food microbiological laboratory.
• Applications of bio-fertilizers in leguminous fodder crops
• Preservation of leguminous/ non-leguminous fodder crops by ensiling
• Enrichment of poor-quality roughages by solid-state fermentation
• Manipulation of rumen microbial ecosystem
• Development of direct-fed microbials for ruminants.
• Screening of prebiotics
• Isolation and screening of potential dairy probiotics
• Development of synbiotic products
• Evaluation lactic acid bacteria for production of functional biomolecules
• Nutritional and therapeutic value of probiotic products
• Enhancing shelf life of foods through microbial fermentation
• Industrial production of metabolites such as recombinant proteins/enzyme in a
bioreactor and down stream processing
• Production of microbial biomass as single cell protein.
• Effect of natural environment on microbial growth and production.
• Studies on bacterial growth kinetics in batch and continuous culture systems.
• The biochemical and genetic regulatory mechanism of sporulation.
• Effect of different nutrients on the growth and production of microorganisms.
• Nutrient transport systems through cell-membrane of yeast and bacteria.
• Metabolic engineering
• Cloning and Expression of prokaryotic and Eucaryotic genes in E. coli and yeast
systems
• Recombinant proteins / enzymes for application in food / dairy industry
• Genomics and Proteomics of lactic acid bacteria
• Biodiversity of Indian probiotic cultures
• Understanding probiotic functionality at molecular level and role as potential
probiotic markers
• Food grade vector systems
• Whole genome shuffling / DNA / Family shuffling
51
• Molecular diagnostics for detection and identification of food pathogens and dairy
micro-organisms.
• Novel bacteriocins of LAB
• Genetic modification of LAB
• Genetic improvement of starter cultures
• Newly emerging pathogens- rapid method of identification
• Food toxins- bioremediation
• Metabolic engineering of LAB.
• Emerging Foodborne pathogens
• Rapid methods for detection and identification of pathogens in milk and milk
products.
• Resistance of Foodborne pathogens to emerging food processing technologies.
• Molecular techniques for detection of Foodborne pathogens and their toxins.
52
DAIRY CHEMISTRY

DC 511 PHYSICOCHEMICAL ASPECT OF MILK CONSTITUENTS 2+1
AND MILK PRODUCTS
DC 512* MILK CARBOHYDRATES, MINERALS AND WATER 2+1
SOLUBLE VITAMINS
DC 513* CHEMISTRY OF MILK LIPIDS 2+1
DC 514 FOOD CHEMISTRY 3+1
DC 521* CHEMISTRY OF MILK PROTEINS 3+1
DC 522* CHEMISTRY OF MILK PRODUCTS 3+1
DC 591 MASTER’S SEMINAR 1+0
DC 599 MASTER’S RESEARCH 20
DC 611 ADVANCES IN CHEMISTRY OF MILK PROTEINS 3+0

DC 612 ADVANCES IN CHEMISTRY OF MILK LIPIDS 3+0
DC 621 ADVANCES IN THE CHEMISTRY OF MILK PROCESSING 3+0
DC 622 ADVANCES IN ANALYTICAL TECHNIQUES IN DAIRY 3+0
CHEMISTRY
DT 622# R & D MANAGEMENT IN DAIRY INDUSTRY 3+0
DC 691 DOCTORAL SEMINAR I 1+0

DC 692 DOCTORAL SEMINAR II 1+0
DC 699 DOCTORAL RESEARCH 45
* Compulsory for Master’s programme;

# Cross-listed with Dairy Technology
53
DAIRY CHEMISTRY
Course Contents
DC 511 PHYSICO-CHEMICAL ASPECTS OF MILK 2+1

CONSTITUENTS AND MILK PRODUCTS
Objective
To impart knowledge in understanding the physico-chemical aspects of
milk and milk products with special reference to their processing and
quality assurance.
Theory
UNIT I
Kinetics: Order and molecularity of a reaction; reactions obeying the
kinetics of zero, 1st, 2nd and 3rd order; kinetics of denaturation of whey
proteins; the role of enzymes as a biological catalyst; factors affecting the
rate of enzyme action- (i) concentration of substrate, (ii) concentration of
enzyme, (iii) concentration of reaction products, (iv) pH, (v) temperature,
(vi) time, (vii) activators, and (viii) inhibitors; concept of activation energy.
UNIT II
Electrochemistry: Electrolytic dissociation; activity, ionic strength; salt
equilibria in milk; dissociation constant of acids and bases; effect of ionic
strength on dissociation constant; buffers; limitation of buffers; Good's
buffer; buffer capacity and buffer index of milk; electrode potential;
concentration cells; ion-selective electrodes.
UNIT III
Surface chemistry: Adsorption at solid-vapour (gas) inter-phase; monolayer
and multilayer adsorption; capillary condensation; adsorption isotherms;
hysteresis; adsorption at solid-liquid and liquid-liquid interphase; Gibb's
equations. water activity: sorption of water on milk constituents and milk
products- its relation to stability of dairy products.
UNIT IV
Interfacial tension; micelles-- definition, critical micelle concentration -
formation and stability; emulsions-, foams- and gels--their formation, struc-
ture and stability; importance of these phenomena in milk and milk
products.
UNIT V
Properties of colloidal systems with special reference to electrical, optical,
hydrodynamic (shape and hydration) and rheological aspects; colloidal
stability of casein micelles; membrane phenomenon and Donnan membrane
effect.
Practical
Determination of the order of hydrolysis of an ester/carbohydrate and
measurement of activation energy; determination of the progress curve
obtained during the hydrolysis of P-nitrophenyl phosphate by milk alkaline
phosphatase; determination of the Michaelis constant for the digestion of
casein by trypsin; preparation of a Tris/phosphate/citrate buffer of a given
molarity/ionic strength and pH; determination of pH of the buffer;
estimation of calcium ions of milk using an ions selective electrode;
determination of viscosity of condensed milk using falling ball viscometer;
measuring the stability of an oil-in-water emulsion stabilised by milk
proteins; foaming capacity and foam stability of caseins/whey proteins;
54
drawing of an adsorption isotherm of water on casein.
Suggested Readings
Damodran S & Paraf A. 1997. Food Proteins and their Applications.
Marcel Dekker.
Fox PF & Mc Sweeney PLH. 1998. Dairy Chemistry and Biochemistry.
Blackie.
Freifelder D. 1982. Physical Biochemistry: Application to Biochemistry and
Molecular Biology. WH Freeman.
Mitchell JR & Ledward DA. 1986. Functional Properties of Food
Macromolecules. Elsevier.
Moore WJ. 1983. Basic Physical Chemistry. Prentice Hall.
Ronald J.1987. Physical Properties of Foods. Elsevier.
Walstra P & Jenness R. 1984. Dairy Chemistry and Physics. John
Wiley & Sons
Wong NP, Jenness R, Keeney M & Elmer HM. 1988. Fundamentals of
Dairy Chemistry. 3rd Ed. Van Nostrand Reinhold Co.
DC 512 MILK CARBOHYDRATES, MINERALS AND 2+1

WATER SOLUBLE VITAMINS
Objective
To impart basic knowledge on all aspects of milk carbohydrates, minerals
and water soluble vitamins and to project the importance of these milk
constituents on the quality of milk and milk products as well as in human
health.
Theory
UNIT I
Lactose: Occurrence, isomers, molecular structure.
UNIT II
Physical properties: crystalline habits, hydrates, lactose glass, equilibrium
of different isomers in solution, solubility, density, sweetness.
UNIT III
Chemical properties: Hydrolysis, pyrolysis, oxidation, reduction,
degradation with strong bases, derivatives, dehydration and fragmentation,
browning reactions and its mechanism.
UNIT IV
Minerals: Major and miner minerals, factors associated with variation in
salt composition.
UNIT V
Physical equilibrium amongst milk salts, partitioning of salt constituents
and factors affecting it. Effect of various treatments on salt equilibrium.
UNIT VI
Salt balance and its importance in the processing of milk, protein mineral
interaction, distribution and importance of trace elements in milk.
UNIT VII
Water soluble vitamins: Thiamin, riboflavin, niacin, pantothenic acid,
pyridoxine, biotin, folin and cyanocobalamin.
UNIT VIII
Molecular structures of these vitamins, levels in milk and milk products,
biological significance, factors affecting their levels.
55
UNIT IX
Ascorbic acid: structure and its relation to Eh of milk and milk products.
Practical
Estimation of lactose in milk by volumetric, gravimetric, ploarimetric and
colorimetric methods. Estimation of sodium and potassium by flame
photometry, calcium and magnesium by EDTA method, phosphorus by
colorimetric (Fiske and Subbba Rao) method. Citric acid and iron by
colorimetric methods, vitamin C in milk (volumetric method) and brown
colouring matter/burnt particles in milk powder.
Suggested Readings
Fox PF.1985. Developments in Dairy Chemistry. Vol. III. Applied Science
Publ.
Jennes RG.1995. Handbook of Milk Composition. Academic Press.
Jenness R & Patton S. 1969. Principles of Dairy Chemistry. John Wiley &
Sons.
Walstra P & Jenness R. 1984. Dairy Chemistry and Physics. John & Wiley.
Wong NP, Jenness R, Kenney M & Elmer MH. 1988. Fundamentals of
Dairy Chemistry. Van Norstand Reinhold Co.
DC 513 CHEMISTRY OF MILK LIPIDS 2+1

Objective
To impart the basic knowledge on all aspects of milk lipids and to project
the importance of milk lipids in the quality of milk products as well as in
human health.
Theory
UNIT I
Milk lipids: classification, gross composition and physical properties;
neutral and polar lipids and their role in milk and milk products.
UNIT II
Fatty acids profile: composition, properties and factors affecting them.
UNIT III
Unsaponifiable matter: composition with special reference to sterols and fat
soluble vitamins and caretenoids, chemistry, physiological functions and
levels of milk.
UNIT IV
Chemical properties: hydrolysis by alkali, water and enzymes;
hydrogenation and halogenation; transesterification and interesterification;
oxidation by chemical reagents.
UNIT V
Auto-oxidation: Definition, theories, induction period, secondary products
of auto oxidation, factors affecting, prevention and measurement;
antioxidants: Definition, types, reaction mechanism and estimation.
Practical
Determination of melting point/slip point, peroxide value, TBA value,
carbonyl value, unsaponifiable matter; estimation of total cholesterol by
direct and indirect method; vitamin A, total phospholipids and free fatty
acids in ghee; preparation of fatty acid esters and their analysis on GLC;
estimation of antioxidants such as BHA.
56
Suggested Readings
Akoh CC & Min DB. 1997. Food Lipids: Chemistry, Nutrition and
Biotechnology. Marcel Dekker.
Fox PF. 1983. Development in Dairy Chemistry. Vol. II. Applied Science
Publ.
Mathur MP, Datta Roy D & Dinakar P. 1999. Text Book of Dairy
Chemistry. ICAR.
Sons.
Wong NP, Jenness R, Keeney M & Elmer HM. 1988 Fundamentals of
Dairy Chemistry. Van Nostrand Reinhold Co.
DC 514 FOOD CHEMISTRY 3+1

Objective
To impart knowledge on different aspects of food components.
Theory
UNIT I
Forms of water in foods, water solute interactions and food stability, solute
mobility and food stability; role of ice in the stability of food at sub-
freezing temperatures.
UNIT II
Forms, swelling, gelatinization; food applications and role of starch in
bread making; modification of starches for industrial applications, physico-
chemical changes taking place during malting, mutual interactions of
hydrocolloids and interactions with proteins and lipids; role of
hydrocolloids in different food preparations.
UNIT III
Functional properties of food proteins; structure-function relationship and
their modifications, denaturation of food proteins; effect of pressure on
food proteins, enzymes & their application in food industry; physico-
chemical properties of food lipids and their modifications.
UNIT IV
Indigenous and synthetic food pigments; legal requirements for food
colourants; flavour compounds of different foods and flavour enhancers
UNIT V
Changes taking place during fermentation; drying and roasting of chocolate
and cocoa; chemistry of tea manufacture; composition of coffee beans;
physico-chemical changes during roasting of coffee beans.
Practical
Determination of level of artificial sweeteners and crude fiber in food
products; starch in flour by polarization method; total amino acids and
polyphenols in lemon juice; fat in grains; proteins in flour; tannins in
coffee/tea, caffeine content in coffee; HMF in honey, and visit to a food
industry.
Suggested Readings
Belitz HD & Grosch W. 1999. Food Chemistry. 2nd Ed. Spinger Verlag.
Fennema OR. 1985. Food Chemistry. Marcer Dekker.
Srinivas D & Alan Praf. 1997. Food Proteins and their Applications.
Marcel Dekker.
57
Turker GA & Woods LFJ. 1995. Enzymes in Food Processing. Blackie.
Williams PA & Phillips GO. 2000. Gums and Stabilizers for the Food
Industry. Royal Society of Chemistry.
DC 521 CHEMISTRY OF MILK PROTEINS 3+1

Objective
To impart knowledge on different aspects of milk proteins.
Theory
UNIT I
Distribution and fractionation of different nitrogen fractions, non-protein
nitrogen constituents, nomenclature of milk proteins.
UNIT II
Major milk proteins: caseins (acids and micellar), methods of isolation;
fractionation of casein and heterogeneity, physico-chemical properties,
glycosylation, phosphorylation, amino acid composition, primary and
secondary structure of different fractions; casein micelle models.
UNIT III
alpha-Lactalbumin and beta-lactoglobulin - distribution and methods of
isolation; whey protein concentrates and their functional properties.
UNIT IV
Denaturation of caseins and whey proteins as affected by change of
temperature, pH and additives; casein-whey protein interactions; genetic
polymorphism and biosynthesis of milk proteins.
UNIT V
Minor milk proteins: proteose-peptone, immunoglobulins, lactoferrin,
lipoprotein and fat globule membrane proteins.
UNIT VI
Milk enzymes: properties and their significance with particular reference to
lipases, phosphatases, catalase, peroxidase, xanthine oxidase, lysozyme,
lactoperoxidase and galactosyl transferase.
Practical
Estimation of different nitrogen fractions of milk by Kjeldahl method. Milk
protein estimation by Folin method. Isolation of acid and micellar casein;
urea fractionation of acid casein; separation of amino acids using thin
layer/paper chromatography; isolation of alpha-lactalbumin and beta-
lactoglobulin by ammonium sulphate precipitation; polyacrylamide gel
electrophoresis of milk proteins; estimation of milk enzymes like lipase,
alkaline phosphatase and lactoperoxidase; fractionation of milk proteins by
molecular sieving; estimation of hexoses and sialic acid in casein.
Suggested Readings
Fox PF. 1982. Developments in Dairy Chemistry. Vol. I. Proteins. Applied
Science Publ.
Fox PF & McSweeney PLH. 1998. Dairy Chemistry and Biochemistry.
Blackie Academic Professional, Champman & Hall.
Fox PF. 1992. Advanced Dairy Chemistry. Elsevier.
Mathur M., Datta Roy D & Dinakar P. 1999. Text Book of Dairy
Chemistry. ICAR.
Robert G Jensen 1991. Handbook of Milk Composition. Academic Press.
Walstra P & Jenness R.1984. Dairy Chemistry and Physics. John Wiley &
Sons.
58
Wong NP, Jenness R, Keeney M & Elmer HM. 1988 Fundamental of Dairy
Chemistry. 3rd Ed. Van Nostrand Reinhold Co.
DC 522 CHEMISTRY OF MILK PRODUCTS 3+1

Objective
To project the physico-chemical changes and effects of various milk
constituents of the milk products during manufacture and storage.
Theory
UNIT I
Physico-chemical changes taking place during manufacturing and storage
of concentrated milk; structure and physico-chemical properties of dried
milk as affected by different process treatments; stability of milk powder;
physical properties of instant milk powder.
UNIT II
Role and mechanism of action of stabilizers and emulsifiers in ice cream.
Physiological and biochemical response of infants to human milk and
formulated foods.
UNIT III
Heat stability of concentrated milk as affected by different process
variables; milk constituents and additives; milk clotting enzymes from
different sources (animal and plant); their isolation; purification and action.
UNIT IV
Changes taking place during manufacturing and ripening of cheese;
chemical defects in cheese. Theory and metabolic pathways of
fermentation.
UNIT V
Size distribution of fat globules and factors affecting it; creaming
phenomena; mechanism of churning; physico-chemical characteristics;
grading and standards of butter.
UNIT VI
Genesis of flavour; aroma and texture in ghee; adulteration of ghee and its
detection.
Practical
Determination of lactose and sucrose in condensed milk and ice-cream;
determination of heat stability of milk and its concentrate; determination of
moisture in skim milk powder/infant food by vacuum oven; determination
of fat in cream by Gerber and Rose-Gottlieb methods; determination of
moisture, fat (Gerber method), curd and salt in butter; determination of
diacetyl and acetyl methyl carbinol in butter/cultured products;
determination of RM, Polenske value, iodine value, saponification value of
ghee; determination of nitrite, nitrate, free amino acids and free fatty acids
in cheese; determination of rennet clotting time of milk.
Suggested Readings
Fox PF. 1985. Developments in Dairy Chemistry. Vol. III. Applied Science
Publ.
Gurr MI. 1981. Comparative Aspects of Feeding Human and Artificial Milk
for Infant Feeding. J. Dairy Res.: 48, 519.
ISI. 1981. Handbook of Food Analysis S.P. 18 (Part II) Dairy Products. ISI
Specifications, Manak Bhavan, New Delhi.
59
Milks. 2nd Ed. Blackie Academic and Professional, Chapman &
Hall.
Mathur MP, Roy DD & Dinakar P.1999. Textbook of Dairy Chemistry.
ICAR.
Official methods of A.O.A.C. 11th and 15th Eds.
Prevention of Food Adulteration Act (PFA) 1954 and PFA Rules1955.
Sons.
Wong NP, Jeness R, Keeney M & Elmer HM. 1988. Fundamentals of
Dairy Chemistry. Van Nostrand Reinhold Co.

Objective
To project the importance of chemical quality assurance and quality control
in relation to dairy industry and impart basic knowledge on all aspects of
chemical quality assurance.
Theory
UNIT I
Concept of quality assurance and quality control in relation to dairy
industry; quality management systems - ISO 9000; total quality
management (TQM); hazard analysis of critical control points (HACCP);
good manufacturing practices (GMP); role of international organisations
such as ISO; IDF; CAC; AOAC; WTO and national organisations like BIS;
CCFS.
UNIT II
PFA and Agmark; significance of milk and milk products order (MMPO)
and APEDA (Agricultural and Processed Foods Export Development
Authority) in dairy industry; guidelines for setting up quality control
laboratory; sampling of milk and milk products; dairy detergents and
sanitizers; calibration of milk testing glassware; preparation of standard
reagents.
UNIT III
Instrumentation in analysis of milk and milk products; detection of
adulterants in milk and milk products; packaging materials in relation to
quality assurance of dairy products.
UNIT IV
Chemical residues: pesticides; antibiotics; heavy metals; radionuclides etc;
in dairy products.
Practical
Preparation of standard solutions and buffers; testing of available chlorine
content in hypochlorites/bleaching powder; determination of purity of
common salt for butter and cheese making; detection of common
adulterants in milk and foreign fat/oil in ghee; checking the calibration of
lactometers; hydrometers; butyrometers; milk pipette and thermometer;
qualitative colour tests to distinguish between azo dyes and natural dyes in
butter; detection of pesticide residues and antibiotics in milk.
Suggested Readings
AOAC. 2000. Official Methods of Association of Official Analytical
Chemists, USA.
60
IDF 1997. Monograph on Residues and Contaminants in Milk and Milk
Products. Special Issue No. 9701.
IDF 1993. Quality Assurance (QA) and Good Lab. Practices (GLP) in
Dairy Laboratories. Special Issue No. 9302.
ISI 1981. Handbook of Food Analysis S.P. 18 (Part II) Dairy Products. ISI
Specifications, Manak Bhavan, New Delhi.
Prevention of Food Adulteration Act (PFA)1954 and PFA Rules 1955. 10th
Ed. Aug. 2006.
Ralph Early. 1995. Guide to Quality Management System for Food
Industry. Blackie..

Objective
To impart the advanced knowledge on the use of analytical techniques in
Dairy Chemistry.
Theory
UNIT I
Electrophoresis: principle and types, isoelectricfocussing.
UNIT II
Chromatography: column, TLC, GLC, HPLC, gel-permeation, ion-
exchange, affinity.
UNIT III
Spectrophotometry: UV, visible, IR and flame photometry; potentiometry:
principle, various electrodes; electrometric measurements of pH, buffers.
UNIT IV
Radiotracer technique: nuclear transformation, nuclear decay, measurement
of radioactivity and safety precautions for radioactive materials.
UNIT V
Membrane processes; ultracentrifugation.
Practical
Preparation of methyl esters of fatty acids of milk fat followed by their
GLC separation and estimation; TLC separation of amino acids; gel-
filtration of biomolecules; preparation of a buffer and measurement of its
pH electro-metrically and using indicators; SDS gel electrophoresis and
molecular weight determination; determination of absorption spectra of
BSA and demonstration of Beer's law; determination of sodium and
potassium by flame photometry; ultracentrifugal preparation of micellar
casein, separation of milk proteins using ion-exchange chromatography.
Suggested Readings
Clark JM & Switzer RL. 1977. Experimental Biochemistry. WH Freeman
& Co.
Cooper TG. 1977. The Tools of Biochemistry. John Wiley & Sons.
Frank A Settle.1997. Handbook of Instrumental Techniques for Analytical
Chemistry. Prentice Hall.
Nielsen S Suzanne 1994. Introduction to the Chemical Analysis of Foods.
Jones & Barlett Publ.
Sawhaney SK & Singh R. 1985. An Introduction to Practical Biochemistry.
Narosa Publ.
Stock R & Rice F. 1974. Chromatographic Methods. Chapman & Hall.
61
Wilson K & Walker J. 2000. Practical Biochemistry: Principles and
Techniques. Cambridge Univ. Press.
DC 611 ADVANCES IN CHEMISTRY OF MILK PROTEINS 3+0

Objective
To impart knowledge on different aspects of milk proteins
Theory
UNIT I
Biosynthesis of milk proteins, proteins and lipoproteins of milk fat globule
membrane (MFGM).
UNIT II
Amino acid sequence of caseins, structure - function relationship of casein
and whey protein; association - dissociation equilibria.
UNIT III
Physical, chemical and enzymatic modification of milk proteins and their
functional characteristics.
UNIT IV
Mechanism of action and biological role of specific and non-specific
antimicrobial factors in milk - immunoglobulins, lactoferrin,
lactoperoxidase and lysozyme.
UNIT V
Milk derived bioactive peptides - their properties; significance and
application; bitter peptides in cheese; growth factors in milk; transgenic
milk proteins and their significance.
UNIT VI
Therapeutic and allergy aspects of milk proteins; protein films and
coatings; their properties and applications.
Suggested Readings
Damodaran S & Paraf A. 1997. Food Proteins and their Applications.
Marcel Dekker.
Fields Cregg B. 1997. Solid Phase Peptide Synthesis: Methods in
Enzymology. Vol. 289. Academic Press.
Fox PF. 1995. Advanced Dairy Chemistry. Vol. 1. Proteins. Applied
Science Publ.
IDF. 1993. Proceedings of Seminar on Indigenous Antimicrobial Agents of
Milk - Recent Developments. Uppasala, Sweden.
Popay AI & Prosser CG.1997. Biotech in Agric. Series No. 18, CABI.
Visser Hans. 1992. Protein - Interactions. VCS.
Welch RAS, Burns DJW & Davis SR. 1997. Milk Composition, Production
and Biotechnology. CABI.
DC 612 ADVANCES IN CHEMISTRY OF MILK LIPIDS 3+0

Objective
To impart the advanced knowledge on different aspects of milk lipids with
special reference to their relation with human health.
62
Theory
UNIT I
Origin, composition, structure and physical chemistry of milk fat globule
membrane. Comparative aspects of milk lipids from different species such
as human, bovine, buffalo, sheep, goat, pig and camel.
UNIT II
Lipolytic enzymes from different species including human; bile salts;
stimulated lipase and esterases. Biosynthesis of fatty acids; glycerol;
neutral lipids; phospholipids; sphingolipids; cholesterol.
UNIT III
Essential fatty acids, prostaglandins and flavor compounds. Conjugated
linoleic acids – different isomers, factors affecting their levels in dairy
products and their significance.
UNIT IV
Chemistry of oxygen in relation to auto-oxidation of milk fat including
effect of milk components and environmental factors, thermal oxidation,
chemical and biological properties of heated and oxidized fats.
UNIT V
Significance of milk lipids in human health. Role of milk lipids in
consumer acceptance of dairy products. Polymorphism and milk fat
crystallization.
Suggested Readings
Bindra JS & Bindra R.1977. Prestoglandins Synthesis. Academic Press.
Fox PF. 1995. Advanced Dairy Chemistry. Vol. II. Lipids. 2nd Ed. Chapman
& Hall.
Blackie Academic Professional, Champman & Hall.
Gurr MI. 1992. Role of Fats in Food and Nutrition. Elsevier.
Sons.
Wong NP, Jenness R, Keeney M & Elmer HM. 1988. Fundamental of
Dairy Chemistry. 3rd Ed. Van Nostrand Reinhold Co.
DC 621 ADVANCES IN CHEMISTRY OF MILK PROCESSING 3+0

Objective
• To highlight the impact of processing parameters on the milk
constituents with special reference to chemical changes involved.
• To impart the knowledge on the status and chemistry of contaminants in
milk and milk products.
• To impart the basic knowledge on the chemistry and significance of
additives.
Theory
UNIT I
Heat induced changes and interactions between protein, lipids,
carbohydrate and minerals during processing of milk. Effect of heat on the
proteins of concentrated milk systems. Inactivation of milk indigenous
enzymes during processing.
UNIT II
Milk fat replacers, chemistry of artificial sweeteners and fortified milk.
63
UNIT III
Physical changes in the fat globules in unhomogenized and homogenized
milk; cold agglutination – its mechanisms and role.
UNIT IV
Specific and non – specific enzymatic coagulation of milk.
UNIT V
Status and formation of bioactive peptides in fermented milk products.
UNIT VI
Chemistry involved in high pressure processing of milk.
UNIT VII
Radio nuclides, drugs, pesticides, and polybiphenyls in milk.
Suggested Readings
Colette Shortt & John O’ Brien. 2004. Handbook of Functional Dairy
Products. CRC Press.
Blackie Academic Professional, Chapman & Hall.
IDF. 1989. Bulletin 238. Intern. Dairy Fed., Brussels.
IDF. 1997. Special issue 9701. Intern. Dairy Fed., Brussels.
IDF.1995. Special issue. Heat Induced Changes in Milk. Intern. Dairy Fed.,
Brussels.
Leo ML Nollet. 2004. Intense Sweeteners. Handbook of Food Analysis. 2nd
Ed. Marcel Dekker.
Mathur MP, Roy DD & Dinakar P. Textbook of Dairy Chemistry. ICAR.
Nabors Lyn O’Brien. Alternative Sweeteners. 3rd Ed. Marcel Dekker.
DC 622 ADVANCES IN ANALYTICAL TECHNIQUES IN 3+0

DAIRY CHEMISTRY
Objective
To highlight the importance of modern analytical techniques used for
analysis of milk and milk products.
Theory
UNIT I
Isoelectric focusing and 2-D polyacrylamide gel elctrophoresis (PAGE),
Capillary zone electrophoresis, Blotting technique.
UNIT II
Differential scanning calorimetry, Radio immuno assay (RIA), Enzyme
linked immunosorbent assay (ELISA).
UNIT III
High performance liquid chromatography (HPLC), Circular dichroism
(CD), Protein sequencing, X-ray crystallography.
UNIT IV
Atomic-, Mass-, Infrared-, Fluorescence- Spectroscopy.
Suggested Readings
Blundell TL & Johnson LN.1976. Protein Crystallography. Academic
Press.
Calter P. 2004. Methods in Molecular Biology. Vol. 244 2nd Ed. Protein
Purification Protocols. Humana Press.
FL Creighton T.1998. Protein Structure. 2nd Ed. Portland Press.
Nielsen SS. 1994. Introduction to Chemical Analysis of Foods. Part IV.
Jones & Bertlett Publ.
64
Oliver RWA.1989. HPLC of Macromolecules - A Practical Approach. IRL
Press.
Sawyer L. et al. 2002. Milk Protein Structure-What can it tell the dairy
industry? International Dairy Journal 12: 299-301.
Settle F.1997. Handbook of Instrumental Techniques for Analytical
Chemistry. Hall International.
Smith BJ. 1996. Protein Sequencing Protocols, Methods in Molecular
Biology. Vol. 32. Humanta Press.
Swadesh J.1997. HPLC - Practical and Industrial Applications. CRC Press.
65
DAIRY CHEMISTRY
List of Journals
• Analyst
• Analytical Biochemistry
• Australian Journal of Dairy Technology
• Bulletin of Environmental Contamination and Toxicology
• Dairy Science and Technology
• Environmental Science and Technology
• Food Additives and Contaminants
• Food Chemistry
• Indian Dairyman
• International Dairy Federation Bulletin
• International Journal of Food Composition
• International Journal of Food Science and Technology
• Journal of Agricultural and Food Chemistry
• Journal of American Oil Chemist Society
• Journal of Association of Official Agricultural Chemists
• Journal of Biochemistry
• Journal of Chromatography
• Journal of Dairy Technology
• Journal of Food Quality
• Journal of Food Safety
• Journal of Lipid Research
• Lipids
• LWT-Food Science and Technology
• Milchwissenschaft-Milk Science International

• Product/process improvement and quality assessment
• Development of new dairy based products
• Formulation of value added dairy products
• Optimization of processes for enhancing quality of dairy products
• Status of lactose in traditional dairy products including milk sweets.
• Status and role of minerals in Indian dairy products with particular reference to
buffalo milk products.
• Rapid methods for detection of animal body fats in ghee.
• Survey on the changes of physico - chemical parameters of milk fat.
• Structural aspects of milk lipids in relation to processing.
66
• Interactions of major milk constituents with different food ingredients during
manufacture of value added products.
• Analysis of artificial sweeteners and their stability in dairy products.
• Characterization of bioactive proteins and peptides in milk.
• Structural and kinetic studies on milk proteins and peptides.
• Establishing antioxidative potential of milk and milk protein products for
promoting them as health foods.
• Development of commercially viable separation technologies for fractionation of
colostrum/milk proteins for value addition.
• Characterization and evaluation of milk protein products as neutraceutical.
• Milk protein products for value addition in foods.
• Biotechnological applications of milk proteins
• Development of newer milk products by replacing partially or wholly some of the
milk constituents.
• Projects to enhance the shelf life of milk products through process modification,
packaging and antioxidants.
• Applications of instruments in the quality control of milk and milk products.
• Detection of adulterants in milk and milk products.
• Analysis of chemical residues: pesticides; antibiotics; heavy metals etc; in dairy
products.
• Isolation and purification of growth factors and bioactive peptides from colostrum,
milk and whey.
• Interaction of caseins and whey proteins during processing of milk
• Enzymatic modification of milk proteins and their incorporation in to food system
• Structure and functional properties of milk proteins during processing
• Use of antimicrobial factors of milk in formulation of value added products.
• Development of enzyme based new markers for assessing quality of thermally
processed milk.
• Development of biosensors for rapid and on-line detection of chemical constituents
including contaminants, adulterants and toxins in milk and milk products.
• Increasing CLA content in milk and milk products.
• Establishing authenticity of milk fat purity.
• Interaction between different components during processing
• Fractionation of milk fat
• Isolation, purification and characterization of bioactive peptides generated during
fermentation of milk
• Structural and functional stability of bioactive peptides.
• Chemistry of cheese made with recombinant chymosin.
• Analysis of artificial sweeteners and their stability in dairy products
• Analysis of contaminants viz. pesticide residues, antibiotics residues, heavy metal,
etc. in milk and milk products.
67
DAIRY ENGINEERING

DE 511* DAIRY AND FOOD ENGINEERING-I 3+0
DE 512* HEAT TRANSFER 3+0
DE 513 TRANSPORT PHENOMENA 2+0
DE 514 REFRIGERATION ENGINEERING 2+1
DE 515* DESIGN OF PROCESS EQUIPMENT 3+0
DE 521* DAIRY AND FOOD ENGINEERING-II 3+0
DE 522 BIO-THERMAL PROCESS ENGINEERING 3+0
DE 524 ENVIRONMENTAL ENGINEERING 2+0
DE 525* INSTRUMENTATION AND PROCESS CONTROL 2+1
DE 591 MASTER’S SEMINAR 1+0
DE 599 MASTER’S RESEARCH 20
DE 611 ADVANCES IN DAIRY PROCESS ENGINEERING 3+1

DE 612 ADVANCED HEAT TRANSFER 3+0
DE 621 COMPUTATIONAL METHODS AND SIMULATION IN 2+0
DAIRY & FOOD ENGINEERING
DE 622 PHYSICO-CHEMICAL PROCESSES 3+0
DE 690 SPECIAL PROBLEMS 0+2
DE 691 DOCTORAL SEMINAR I 1+0

DE 692 DOCTORAL SEMIANR II 1+0
DE 699 DOCTORAL RESEARCH 45
* Compulsory for Master’s programme

68
DAIRY ENGINEERING
Course Contents
DE 511 DAIRY AND FOOD ENGINEERING-I 3+0

Objective
To disseminate the knowledge of properties of products and unit operations
involved in dairy and food engineering
Theory
UNIT I
Engineering properties of dairy and food materials and their significance in
equipment design; processing and handling of dairy and food products.
UNIT II
Concept of rheology: ideal elastic, plastic and viscous behaviour,
viscoelasticity, rheological models and constitutive equations, Maxwell
model, Kelvin model and Burgers model, viscoelastic characteristion of
materials, stress-strain behavour, creep, stress relaxation, non-Newtonian
fluids and viscometry.
UNIT III
Rheology and texture of food materials: methods of texture evaluation,
subjective and objective measurements, mechanical tests, firmness,
hardness, dynamic hardness, objective methods of measuring texture,
rheological properties of dairy products, strength of food materials.
UNIT IV
Aerodynamic and hydrodynamic characteristics: drag coefficient, terminal
velocity and Reynold’s number, application of aerodynamic properties to
the separation, pneumatic handling and conveying of foods.
UNIT V
Design of single and multi-effect evaporators: design of spray dryer and its
components, separation and recovery of dried product, design of recovery
system, selection and design of auxiliary equipment.
Suggested Readings
Charm SE. 1971. The Fundamental of Food Engineering. AVI Publ.
Heldman DR & Singh R.P. 1984. Food Process Engineering. The AVI
Publ.
Mohsenin NN. 1986. Physical Properties of Plant and Animal Materials.
Gorden & Breach Science Publ.
Rao MA & Rizvi SSH. 1986 Engineering Properties of Foods. Marcel
Dekker.
Watson EL & Harper IC. 1989. Elements of Food Engineering. AVI Publ.
DE 512 HEAT TRANSFER 3+0

Objective
To develop competence in Heat Transfer Analysis.
Theory
UNIT I
One-dimensional steady state heat conduction through fins (Extended
surfaces): actual and approximate solution. Efficiency, effectiveness and
design of profile area of fins.
69
UNIT II
Two-dimensional steady state heat conduction: analytical and numerical
solution.
UNIT III
Unsteady state heat conduction: analytical solution.
UNIT IV
Forced convection heat transfer in flow over a flat surface: hydrodynamic
and thermal boundary layer, continuity equation, momentum equation and
energy equation, heat transfer coefficient/ Nusselt number in laminar and
turbulent region of boundary layer. Stanton number; Coulburn Analogy;
Empirical co-relations.
UNIT V
Forced convection heat transfer in flow through tubes: Nusselt number in
the entrance region and fully developed laminar and turbulent region.
UNIT VI
Condensation and Boiling Heat transfer: Film wise condensation on vertical
surface; Nusselt equation, Boiling liquids.
UNIT VII
Heat Exchangers: classification, overall heat transfer coefficient.
Performance analysis of parallel flows and counter flow heat exchangers:
LMTD and effectiveness-NTU approach. Use of computer software for
process heat transfer applications.
Suggested Readings
Domkundwar S & Arora SC. 1979. A Course in Heat and Mass Transfer.
Dhanpat Rai & Sons.
Gupta CP & Rajendra Parkash. 1986. Heat Transfer. Nem Chand & Bros.,
Roorkee.
Holman JP & Ganesan. 2002. Heat Transfer. Tata McGraw Hill.
Incropera FP & Dewitt DP. 1996. Fundamentals of Heat and Mass
Transfer. John Wiley & Sons.
Kreith F. 1965. Principles of Heat Transfer. International Text Book Co.
Kumar DS. 2002. Heat and Mass Transfer. S. K. Kataria & Sons, New
Delhi.
DE 513 TRANSPORT PHENOMENA 2+0

Objective
To develop competence in momentum, energy and mass transfer analysis.
Theory
UNIT I
Introduction to transport phenomena – Molecular transport mechanism,
transport properties and their proportionality constants in momentum,
energy and mass transfer.
UNIT II
Steady-state equations - Momentum transport equations for Newtonian and
non-Newtonian fluids, continuity equation in different co-ordinates.
UNIT III
Equations of motion - Navier–Stokes equations and their application in
viscous fluid flow between parallel plates and through pipes.
70
UNIT IV
Turbulent transport mechanism -- Mathematical analysis; eddy viscosity
and eddy diffusivity; velocity, temperature and concentration distribution;
time smoothing equations. Inter-phase transport in isothermal system -
friction factors for various geometries.
UNIT V
Mass transfer -- Fick’s law of diffusion, diffusion of gases and liquids
through solids, equimodal diffusion, isothermal evaporation of water into
air, mass transfer coefficients.
UNIT VI
Dimentional analysis – Buckingham Pi-theorem and matrix method,
application to transport phenomena, analysis among mass, heat and
momentum transfer, Reynolds’ analogy.
UNIT VII
Boundary layer concept - Theoretical and exact solutions for heat, mass
and momentum transfer.
Suggested Readings
Bird RB. 1994. Transport Phenomena. John Wiley & Sons.
Treybal RE. 1968. Mass Transfer Operations. McGraw Hill.
Yuan SW. 1969. Foundations of Fluid Mechanics. Prentice Hall of India.
DE 514 REFRIGERATION ENGINEERING 2+1

Objective
To impart knowledge of design, construction, operation, control and
maintenance of commercial refrigeration systems: cold storages and Air
conditioning plants.
Theory
UNIT I
Vapour compression refrigeration system: major components and their
different types; theoretical vapour compression cycle, theoretical COP;
Effect of operating parameters on COP; actual vapour compression cycle;
multi-pressure commercial refrigeration systems.
UNIT II
Vapour absorption refrigeration system: Ammonia-Water system, Vapour
absorption refrigeration cycle and its representation on Enthalpy-
composition diagram; Absorption system calculations.
UNIT III
Heat Pumps: different ‘heat pump circuits’; analysis of heat pump cycle;
Use of heat pumps in dairy plant for energy conservation.
UNIT IV
Non-conventional refrigeration systems: Thermo electric refrigeration,
vortex tube, cooling by adiabatic demagnetization.
UNIT V
Design elements of Refrigeration equipments: compressor condenser,
evaporator, cooling tower, spray pond etc. Balancing of different
components.
UNIT VI
Design of cold storage and air-conditioning systems: types of cooling loads
and their calculation, design of cold storage for food products, construction
71
of cold storage, equipment selection, insulating materials, vapour barriers,
Ice bank tank.
UNIT VII
Control and maintenance of a commercial refrigeration plant: Pressure
regulating valves, Thermostatic valves, LP/ HP cutouts, high to low side
bypass valve, condenser water regulating valve, capacity control devices,
pump down control, defrosting methods, liquid charging; General
preventive maintenance of refrigeration plant.
Practical
• To find and compare the theoretical and actual COP of a small
refrigeration unit on Refrigeration Tutor.
• Study and design of refrigeration components of a bulk milk chiller
• Visit to a commercial refrigeration plant for cold storage/ ice bank unit
and calculation of its theoretical COP by making cycle on P-h chart.
• Calculation of theoretical work and comparing it with actual work for
some specified cooling job in a commercial plant.
• Study of various control and safety devices in a commercial
refrigeration plant.
• Design problems on cold storage for different food/ dairy products.
• Use of Computer software specific to cold store AC design
• Study the working of an actual heat pump system.
Suggested Readings
Ananta Krishnan CP & Simha NN. 1987. Technology and Engineering of
Dairy Plant Operation. Luxmi Publ.
Andrew D Althhouse & Carl H. Turnquist 1958. Modern Refrigeration and
Air-conditioning. Good Heart Wilcox Co.
Arora CP. 2000. Refrigeration and Air-conditioning. Tata McGraw Hill.
Carrier Air-conditioning. 1965. Handbook of Air-conditioning System
Design. McGraw Hill.
Domkundwar S. 1980. A Course in Refrigeration and Air-conditioning.
Dhanpat Rai & Sons.
Gunther Raymond C. 1957. Refrigeration and Air-conditioning and Cold
Storage. Chilton Co.
Jordan RC & Priester GB. 1971. Refrigeration and Air conditioning.
Prentice Hall of India.
Langley BC. 1978. Refrigeration and Air-conditioning. Reston Publ.
New-Comer JL. 1981. Refrigeration and Air-conditioning. Venus Trading
Co.
DE 515 DESIGN OF PROCESS EQUIPMENT 3+0

Objective
To provide basic knowledge of various procedures for the design of dairy
equipment.
Theory
UNIT I
Design of vessels: codes and regulations, design for pressure and
temperature, loading; allowable stress, minimum thickness after forming,
corrosion mechanism, corrosion control, design for internal and external
pressure, cylindrical and spherical shells, formed heads, reinforcement
72
openings, fabrication requirements, inspection, tests and non-destructive
examination, pressure tests, design and stress evaluation.
UNIT II
Design of milk storage tank: horizontal and vertical silos, insulated and
uninsulated, nozzles and mountings.
UNIT III
High-pressure vessels: constructional features, material for high pressure,
multi shell construction, solid walled vessel.
UNIT IV
Supports for vessel: bracket support, leg support, skirt support, saddle
support.
UNIT V
Heat exchangers: shell and tube heat exchangers, construction codes,
general design considerations, U- tube heat exchangers, double pipe
exchanger, scraped surface exchanger, spiral tube exchangers, joints;
welded tube joints, baffles and tube bundles, tube sheet, double tube sheet
construction, plate type heat exchanger; air cooled heat exchangers.
Computer software for design of heat exchanger.
UNIT VI
Design of reactor vessel: material of construction, agitation, classification,
heating systems, design consideration tank coils.
Suggested Readings
Evans FL. 1974. Equipment Design Handbook. Vol II. Gulf Publ.
Foust AS. et. al.1960. Principles of Unit Operations. John Wiley & Sons.
Hesse CR & Ruston JH. 1964. Process Equipment Design. Affiliated East-
West Press.
Joshi MV & Mahajani VV. 1976. Process Equipment Design. Macmillan
India.
DE 521 DAIRY AND FOOD ENGINEERING-II 3+0

Objective
To develop competence in shelf life simulation of dairy products.
Theory
UNIT I
Water activity and states: a thermodynamic quantity, water sorption
isotherms, hysteresis, theories of sorption hysteresis, water activity
measurement methods, water binding, control of water activity and
moisture, principles of IMF and their application.
UNIT II
Permeability and shelf-life: theoretical considerations, permeability to
gases and vapours, measurement methods, permeability of multilayer
materials, permeability in relation to packaging requirements of food
products.
UNIT III
Calculation of shelf life and requirements for packaging, deteriorative
reactions, accelerated testing, relationship between transport properties of
the package and shelf life of packaged products, simulation of product-
package-environment interaction, shelf life simulation for moisture, oxygen
and light sensitive products.
73
UNIT IV
Theory of ultra filtration and reverse osmosis, selection and types of
membrane and properties concentration polarization, mathematical
description of flow through membrane, application and use in dairy
industry.
UNIT V
Microwave energy absorption, physical parameters in microwave heating
processes, heat transfer phenomena, equipment and application in dairy
food industry.
Suggested Readings
Cheryan M. 1998. Ultrafiltration and Microfiltration Handbook.
Technomic Publ.
Duckworth R. 1975. Water Relations in Foods. Academic Press.
Robertson GL. 1992. Food Packaging (Principles and Practices). Marcel
Dekker.
Rockland LB & George F Stewart. 1991. Water Activity: Influence on Food
Quality. Academic Press.
Toledo RT. 1997. Fundamentals of Food Process Engineering. CBS Publ.
DE 522 BIO-THERMAL PROCESS ENGINEERING 3+0

Objective
To teach the students on biological processes associated with food and
dairy industries.
Theory
UNIT I
Introduction to biochemical engineering: Biochemical kinetics, kinetics of
substrate utilization, enzyme reaction, growth of microorganisms,
fermentors, pasteurization and sterilization and thermal destruction.
UNIT II
Design and analysis of fermentation vessels: residence time distribution,
reactors in food processing, reactor types, analysis of reactor systems.
UNIT III
Mixing in reactors: mixing equipments, power consumption, gas-liquid
mixing, liquid-liquid dispersion, solids suspension and solid- liquid mass
transfer. Scale up of mixers and alternative mixing devices.
UNIT IV
UHT systems and recent advances: factors affecting spoilage of food,
Aseptic packaging systems and conditions.
UNIT V
Thermo-bacteriology: Survivor curve, thermal death curve, Arrheneous
curve, techniques for determination of heat resistance of micro organisms,
analysis of thermal resistance data, processing in containers, process time,
lethality, design of batch and continuous sterilisation cycles in vat.
Suggested Readings
Bailey JE & Ollis DF. 1977. Biochemical Engineering Fundamentals.
McGraw Hill.
Blanch HW & Clark DS. 1996. Biochemical Engineering. Marcel Dekker.
Das H. 2005. Food Processing Operations Analysis. Asian Books.
McNeil B & Harvey LM. 2007. Fermentation: A Practical Approach.
Oxford Univ. Press.
74
Pauline MD. 2005. Bioprocess Engineering Principles. Academic Press.
Rudra Pratap. 2006. Getting Started with MATLAB 7. Oxford Univ. Press.
Stumbo. 1965. Thermobacteriology in Food Processing. Academic Press.
DE 524 ENVIROMENTAL ENGINEERING 2+0

Objective
To disseminate the knowledge pertaining to waste treatment in dairy and
food processing plants.
Theory
UNIT 1
Waste water sources, characteristics, standards for disposal of dairy waste
water.
UNIT II
Physical, chemical and biological characteristics of waste water,
measurement of organic content in waste water.
UNIT III
Physical unit operations in waste water treatment: screening, racks, mixing,
flocculation, sedimentation, floatation, elutriation, vacuum filtration &
incineration.
UNIT IV
Chemical unit operations in waste water treatment: reaction kinetics,
chemical precipitation, aeration and gas transfer process, rate of gas
transfer, adsorption & disinfection.
UNIT V
Biological unit operations- aerobic and anaerobic cycles; kinetics of
biological growth, application of kinetics to treatment systems, aerobic
waste treatment, anaerobic waste treatment.
UNIT VI
Air conditioning systems: clean – room air conditioning; important
pollutants of air; properties of particulate matter and air pollution control
methods.
Suggested Readings
Conway R. & Ross RD. 1980. Handbook of Industrial Waste Disposal. Van
Nostrand Reinhold.
Lewis & Athony. 1976. Industrial Air Pollution Control Equipment for
Particulate. CRC Press.
Metcalf & Eddy. 2003. Waste Water Engineering Treatment Disposal and
Reuse. Tata McGraw Hill.
Threkeld. 1970. Thermal Environmental Engineering. Prentice-Hall of
India.
DE 525 INSTRUMENTATION AND PROCESS CONTROL 2+1

Objective
To impart basic knowledge on principles of measurements and process
control, understanding the working and selection of instruments and
devices for simple applications.
Theory
UNIT I
Instrument Terminology: Elements of generalized measurement system,
static and dynamic characteristics of instruments.
75
UNIT II
Transducers: Electrical, mechanical, magnetic and optical transducers for
measurement of process variables like temperature, pressure, flow, level,
consistency, pH and humidity.
UNIT III
Indicating and Recording Devices: Digital indicators, strip and circular
chart recorders.
UNIT IV
Principles of Automatic Process Control: Process characteristics, control
system parameters, discontinuous, continuous and composite control
modes. Final controlling elements, pneumatic and electric controllers.
UNIT V
Introduction to Computer Based Control: Computer based controller, data
logging, supervisory control, flow chart, control system networks, basic
structure and operation of programmable logic controllers (PLCs).
Practical
Study of various transducers for measurement of pressure, flow, level,
humidity; temperature; study the controller and recorder of pasteurizer; the
working of controllers in constant temperature water baths; to make ladder
diagrams and flow sheet diagrams for control logics; to programme a PLC;
computer interface of a PLC.
Suggested Readings
Eckman DP. 1972. Automatic Process Control. Wiley Eastern Pvt. Ltd.
Johnson CD. 2006. Process Control Instrumentation Technology. Prentice-
Hall of India.
Liplak BG. 1995. Process Measurement and Analysis. Butterworth-
Heinman.
Sawhney AK & Sawhney P. 2007. Electrical and Electronic Measurements
and Instrumentation. Dhanpat Rai & Sons.
DE 611 ADVANCES IN DAIRY PROCESS ENGINEERING 3+1

Objective
To learn process parameters estimations and designs of selected dairy
equipment.
Theory
UNIT I
Evaporation: Classification, design of multiple-effect evaporator,
temperature distribution, boiling point elevation, operation, feeding
methods, condensate and air removal, scale formation and removal, heat
and mass balance, vapor recompression.
UNIT II
Drying: Design data, performance and selection and design of dryer.
UNIT III
Mixing of materials: Factors in mixing, types, operation, mixing gas,
liquid and solid, heat transfer in mixers, power requirement, transmission,
scale-up of models.
UNIT IV
Material handling: System and devices, design of screw, belt, flight, apron
conveyors, bucket elevators, power requirements, and applications, feeders
and feeding mechanism.
76
Suggested Readings
Das H. 2005. Food Processing Operations and Analysis. Asian Books.
Fellows PJ. 1988. Food Processing Technology, Principle & Practices.
Ellis Horwood.
Toledo RT. 2007. Fundamentals of Food Process Engineering. Springer.
DE 612 ADVANCED HEAT TRANSFER 3+0

Objective
To develop analytical approach for heat transfer operation
Theory
UNIT I
Steady state one –dimensional problems, Bessel functions, composite
structures, Principal of superposition, Heterogeneous solids. Power series
solutions, Properties of Bessel functions. Extended surfaces. Approximate
solutions for extended surfaces.
UNIT II
Steady state two and three- dimensional problems. Separation of Variables.
Orthogonal functions. Boundary value problems. Characteristic value
problems, Orthogonality of characteristics functions, Fourier series.
Separation of variables; Steady two-dimensional spherical geometry;
selection of coordinates, steady two-dimensional spherical geometry-
Legendre polynomials. Steady three-dimensional geometry.
UNIT III
Unsteady problems- Separation pf Variables. Orthogonal functions.
Distributed systems having stepwise disturbances. Use of one-dimensional
chart. Time- dependent boundary conditions. Duhamel’s superposition
integral. Laplace transforms.
UNIT IV
Heat transfer in turbulent flow: turbulent flow, boundary layer, Prandtl
analogy, temperature distribution in turbulent flow, empirical and practical
correlation for convection heat transfer, heat transfer in packed beds.
Suggested Readings
Holman JP & Ganesan. 2002. Heat Transfer. Tata McGraw-Hill.
James. 1974. Engineering Heat Transfer. John Wiley & Sons.
Kaviany Massoud. 2002. Principles of Heat Transfer. John Wiley & Sons.
Kreith F & Bohn M. 1997. Principles of Heat Transfer. PWS Publ. Co.
DE 621 COMPUTATIONAL METHODS & SIMULATION IN 2+0

DAIRY AND FOOD ENGINEERING
Objective
To develop competence in developing statistical/theoretical models.
Theory
UNIT I
Taylor’s series expansion in development of numerical differentiation
numerical differentiation procedures, forward difference, backward
difference, central difference.
UNIT II
Numerical integration trapezoidal rule, Simpson’s rule, improper integrals,
Gauss-Legrendre Quadretuse method, numerical methods to solve ordinary
differential equations.
77
UNIT III
Euler method, improved Guier method, Runge-Kutta method, Adam’s P-c
method, initial value problems, numerical solution of partial differential
equation: explicit method, implicit method.
UNIT IV
Simulation concept, simulation methods and their limitations, statistical and
theoretical models.
UNIT V
Problem formulation and development of models; solution and validation
of models; data collection; processing and analysis; basic modeling
problems on unit operations involved in dairy and food processing.
Suggested Readings
Franks & Roger GE.1972. Modeling and Simulation in Chemical
Engineering. Willey-Interscience.
Kobayashi H. 1978. Modeling and Analysis: An Introduction to System
Performance Evaluation Methodology. Addision-Wesley Publ.
Krishnamurthy EV & Sen SK. 1976. Computer bases Numerical
Algorithms. Affiliated East-West Press.
Maisel H & Gougnoli G. 1972. Simulation of Discrete Stochastic Systems.
Science Research Assoc.
Reddy RN & Ziegler CA. 1992. FORTRAN-77 with Applications for
Scientists and Engineers. Jaico.
DE 622 PHYSICO-CHEMICAL PROCESSES 3+0

Objective
To develop understanding with advanced processes, their operations and
design
Theory
UNIT I
Adsorption process. Relationship between surface tension and adsorption,
adsorption equilibria and adsorption isotherm. Sorption kinetics in
continuous flow reactors, factors influencing adsorption.
UNIT II
Separation processes. Reverse osmosis. water and solute diffusion,
nanofiltration, microfiltration, membrane properties, concentration
polarization, mathematical description of flow through a membrance,
extractions, super critical fluid extraction.
UNIT III
Electrodialysis. Minimum energy requirements, selective iontransport,
design of an electrodialysis system, ion-exchange process, exchange
materials, kinetics of exchange, exchange isotherm, ion-selectivity.
UNIT IV
Aeration and gas transfer, gas transfer processes, rates of gas transfer, film
transfer, liquid-phase transport.
Suggested Readings
Aiba SH, Arthur E & Mills N. 1973. Bio-Chemical Engineering. University
Press.
Das H. 2005. Food Processing Operations and Analysis. Asian Books.
Rao MA & Rizvi SSH. 1986. Engineering Properties of Foods. Marcel
Dekker.
78
Pre-requisite Courses in Dairy Engineering for M. Tech. (DE) Students
with B. Tech. (Dairy Technology) background
DE 411 DAIRY PLANT MAINTENANCE 2+1

Objective
To develop confidence with the students in handling maintenance issues of
dairy/food plant
Theory
UNIT I
Introduction of course and its relevance, basic concepts of plant
maintenance.
UNIT II
Elements of preventive maintenance program: Equipment data collection,
reporting and recording, principles of lubrication, lubricants and
preparation of lubrication schedule.
UNIT III
Maintenance organization, development of optimum organization.
UNIT IV
Planned overhaul and PERT planning, engineering and general stores,
workshop facilities in relation to the size and types of dairy plants.
UNIT V
Care and maintenance of S.S. surfaces, and insulations, rubber and gasket
materials, properties, grade and their selection.
Practical
Pipe fittings ,belt and chain conveyors, mechanical seals for milk pumps,
method of determining plant performance ,calibration procedure and set-up
for gauges, performance evaluation of HTST and associated components,
upkeep of electric motors and starters, study of IBR, fire and explosives
safety regulations ,study of IS, British SMS and DIN standards for dairy
equipment, trouble shooting of few selected equipments, maintenance
organization of experimental dairy. Estimation of the maintenance cost,
PERT for overhauling – case study and with group discussion, various
records, equipment date card, card file, log books of a dairy plant.,
specification writing for some select machines & their components .
Suggested Readings
Higgins L & Morrow LC. 1977. Maintenance Engineering Hand-Book.
McGraw Hill.
Keating FH. 1959. Chromium-Nickel Austentic Steel. Butterworths
Scientific Publ.
Newcomer JL. 1981. Preventive Maintenance Manual for Dairy Industry.
Venus Trading Co., Anand.
Stanier W. 1959. Plant Engineering Hand-Book. McGraw Hill.
DE 412 STRENGTH OF MATERIALS 2+0

Objective
To develop competence in stress analysis of machine parts.
79
Theory
UNIT I
Review of stresses in machine parts, temperature stresses, principal planes
and stresses, Mohar’s circle of stress.
UNIT II
Bending of beams, stress analysis in beams of two different materials, shear
stresses in beams.
UNIT III
Strain energy in tension, compression, shear, bending and torsion. Impact
loads on tension members, strength of biomaterials.
Suggested Readings
Bose BN. 1973. Problems in Strength of Materials. Bharti Bhawan, Patna.
Divakaran S & Garg VK. 1969. Strength of Materials. Asia Publ.
Junnarkar SB. 1964. Key to Elements of Applied Mechanics. Charaotar
Book Stall, Anand.
Morley A. 1924. Strength of Materials. Longmans.
Popov EP. 1978. Mechanics of Materials. Prentice- Hall of India.
DE 421 MACHINE TOOL ENGINEERING 2+1

Objective
To provide basic knowledge of construction and operation of various
machine tools and metal cutting.
Theory
UNIT I
Principles of metal cutting: Geometry of single point cutting tools, drills
and milling cutters, normal and effective rake, chip formation, shear plane
and shear zone analysis. Cutting tool materials heating of tools and use of
cutting fluids.
UNIT II
Types of grinding machines, grinding wheels, their selection, speed, and
wheel materials, shaper and planer machines.
UNIT III
Types of lathe machines and lathe operations, Turret and Capstan lathes,
lathe tools, types of milling machines, milling cutters, cutting speed,
calculation of milling time.
UNIT IV
Types of drills and drilling machines, sawing machines, abrasive cutting,
power hacksaw, speed and feed for drilling, shaping, milling and lathe
operations.
Practical
Lathe operations such as facing, turning, taper turning and thread cutting,
Use of radial drilling machine. Study the construction and operation of
milling machine, plain milling on milling machine. Study the operation of
a shaper and prepare a square job from a given round bar.
Suggested Readings
Chapman WAJ. 1972. Workshop Technology. Part-III. Edward Arnold.
Gupta KN & Kaushish JP. 1978. Workshop Technology (Theory and
Practice). Vol. II. New Heights, New Delhi.
Raghuvanshi BS. 2003. A Course in Workshop Technology. Vol. II.
Dhanpat Rai & Sons, New Delhi.
80
Sen GC & Bhattacharya A. 1969. Principles of Metal Cutting. Central
Book.
DE 422 UNIT OPERATIONS 2+0

Objective
To impart engineering knowledge of various unit operations related to
agricultural and dairy processing.
Theory
UNIT I
Engineering properties of biological materials – Mechanical, electrical and
thermal properties; aerodynamic characteristics and frictional properties;
application of engineering properties in design and operation of processing
equipment.
UNIT II
Handling of food products – Mechanics of bulk solids, selection of bulk
handling equipment, operation and construction of conveyors and elevators.
UNIT III
Mechanical cleaning and sizing of food products – Size reduction, size
characteristics, particle geometry, energy for size reduction of granular
materials and dry powders, size- reduction equipment, performance
characteristics of size reducers.
UNIT IV
Crystallization – Material and energy balance in crystallizers, principles of
crystal growth, super saturation and nuclei formation, operation of batch
and continuous crystallizers.
UNIT V
Distillation – Flash distillation of binary mixtures, differential distillation,
steam distillation.
UNIT VI
Flow through porous media -- Absorption, extraction and extrusion
operations.
Suggested Readings
Henderson SM & Perry ME. 1976. Agricultural Process Engineering.
AVI Publ.
McCabe WL & Smith JC. 1967. Unit Operations in Chemical Engineering.
McGraw Hill.
Spivakovsky A & Dyachkov V. 1966. Conveyors & Related Equipments.
Peace Publisher, Moscow.
DE 515 DAIRY PROCESS ENGINEERING 2+1

Objective
A pre-requisite course for engineers admitted to masters program in dairy
engineering. To make up the basic knowledge of dairy plant and mechanics
used in dairy industry.
Theory
UNIT I
Hygienic design concepts, sanitary pipes and fittings, CIP system,
corrosion process and their control milking machine, bulk milk coolers,
milk collecting center milk chilling units.
81
UNIT II
Milk reception equipments, milk tanks, stirrers and mixers, pasteurizers,
sterilizers, centrifugation, homogenizer, packaging and filling machines.
UNIT III
Equipments for cheese, ice-cream, butter manufacture, special milk
products, casein, whey, evaporators, dryers, cyclone separators, fluidized
bed dryer.
UNIT IV
Ultra filtration and reverse osmosis, thermo compressor, MVR, drum
dryers, tray dryers, butter churn.
UNIT V
Equipment for indigenous milk product manufacturing. Small capacity milk
processing equipment, village level sterilization and ascetic packaging.
Practical
Identification of hygienic characteristics of pipes and fittings; technical
specifications of milking and storage equipment, equipment for chilling &
pasteurization; features of centrifuges; ice-cream freezers & packing
machine; cheese vat; milking machine; press & packing machine; butter
manufacturing equipment; different types of evaporators; different types of
dryers, design of dairy plants; design of milk collecting and chilling unit.
Suggested Readings
Ahmad T. 1995. Dairy Plant Engineering and Management. Kitab Mahal,
Allahabad.
Newcomer JL. 1981. Preventive Maintenance Manual for Dairy Industry.
Venus Trading Co., Anand.
Kessler HG. 1981. Food Engineering and Dairy Technology. Verlag.
82
DAIRY ENGINEERING
List of Journals
• American Institute of Chemical Engineering

• ASME Journal of Heat Transfer
• Accessories for Mechanical Engineering Shop.
• Biochemical Engineering Journal
• Chemical Age of India
• Chemical Engineer
• Chemical Engineering Progress
• Dairy Science Abstract.
• IDF Monographs
• Industrial Engineering Chemistry
• Institutions of Engineer’s Journal of Mechanical Engineering.
• International Journal of Heat and Mass Transfer
• International Journal of Machine Tools and Manufacture.
• International Journal of Refrigeration
• Journal of Agricultural Engineering
• Journal of American Society of Agricultural Engineers
• Journal of Dairy Research.
• Journal of Dairy Science.
• Journal of Energy, Heat and Mass Transfer
• Journal of Engineering Machines.
• Journal of Food Science & Technology
• Journal of the Institution of Engineers (India)
• Journal of Instrument Society of India
• Journal of Mechanical Engineers
• Journal of Milk Food Technology.
• Journal of Society of Dairy Technology
• Milchwissenschaft
• Science Direct-International Journal of Heat and Mass Transfer
• The Air conditioning and Refrigeration Journal of ISHRAE
83
• Engineering properties of dairy and food materials.

• Aerodynamic properties of food materials.
• Design of evaporators and dryers.
• Evaluation of major heat transfer resistances in dairy equipment and finding the
scope of improvement in design and control of process parameters to minimize
these resistances.
• To study the process of scale formation and finding the scale heat transfer
resistances in various food/ dairy-processing equipment for use in their research
and development.
• Establishment of empirical correlation for heat transfer coefficients in some critical
heat transfer situations where these are not available.
• Development of ‘computer software’ for the ‘performance analysis’ and design of
heat exchanging equipments.
• Identification of waste heat and check the feasibility and devising effective means
of reutilizing it in a Dairy plant
• Simultaneous momentum, energy and mass transfer analysis.
• Measurement of diffusivity
• Velocity profile measurement.
• Development of computer software helpful in design and balancing of refrigeration
components.
• Establishment of heat transfer data for different kinds of refrigerant condensers and
evaporators.
• Finding the least possible work or highest possible COP for a specific cooling
application
• Devising ways of minimizing the gap between theoretical and actual work required
in producing refrigeration.
• Finding least possible energy consumption for refrigeration requirement of a
variety of dairy products.
• Studies on the use of heat pumps in a dairy plant for energy conservation.
• Identification of waste heat and check the feasibility and devising effective means
of reutilizing it in a Dairy plant
• Application of computational methods for simulation of dairy process equipments
• Development of design manuals for various dairy process equipments
• Sanitary and hygienic design of jacketed kettle with agitator for process heat
transfer.
• Water activity characterization of Milk Products.
• Shelf life estimation of moisture sensitive products.
• Shelf life estimation of oxygen sensitive products.
• Design of fermentors
• Design of aeration systems
• Thermal efficiency analysis of UHT systems
• Application of MATLAB in designing bio thermal processes and equipments.
• Design of waste water treatment techniques
• Characterization of dairy waste
• Evaluation of waste disposal system.
84
• Microprocessor-based instrumentation for estimation of equilibrium moisture content in
dairy products
• Model-based fault-detection for process engineering instrumentation
• Food colour measurement, Instrumentation and techniques
• Instrumentation for measurement of engineering properties of dairy and food materials
• Design of pH controller used in process mechanization of dairy product
• Measurement and control of flow using control valve
• Controlled atmosphere storage for enhancing the shelf-life of foods.
• Dryer design for hygroscopic material
• High pressure processing equipments
• Scale-up designs and performance evaluation
• Agglomeration, instantization and particle size evaluation.
• Engineering properties of food materials.
• Heat & mass balancing for evaporators of special duct
• Development of software model for predicting thermal properties of various dairy
products
• Prediction of overall heat transfer coefficient for various dairy applications
• Analysis of heat transfer mechanism in heat exchangers for various dairy
applications
• Development of simulation models for various unit operations in dairy and food
processing.
• Super critical separation of lipids
• Two-phase gas-liquid exchange studies during oxidation
• Liquid-solid separation studies for acid-coagulated products
• Heat distribution on heat exchanger with different heating sources
• Thermal performance evaluation of processing equipment
• Energy optimization for unit production of milk and milk products
• Plant design for higher performance
• Non-destructive methods for quality identification
• Village level sterilization and ascetic packaging
• Small level milk processing equipments.
85
COMPULSORY NON-CREDIT COURSES
(Compulsory for Master’s programme in all disciplines; Optional for Ph.D. scholars)
PGS 501 LIBRARY AND INFORMATION SERVICES 0+1

PGS 502 TECHNICAL WRITING AND COMMUNICATIONS
0+1
SKILLS
PGS 503 INTELLECTUAL PROPERTY AND ITS
1+0
(e-Course) MANAGEMENT IN AGRICULTURE
PGS 504 BASIC CONCEPTS IN LABORATORY TECHNIQUES 0+1
PGS 505 AGRICULTURAL RESEARCH, RESEARCH ETHICS
1+0
(e-Course) AND RURAL DEVELOPMENT PROGRAMMES
PGS 506
DISASTER MANAGEMENT 1+0
(e-Course)
Course Contents
PGS 501 LIBRARY AND INFORMATION SERVICES 0+1
Objective
To equip the library users with skills to trace information from libraries
efficiently, to apprise them of information and knowledge resources, to
carry out literature survey, to formulate information search strategies, and
to use modern tools (Internet, OPAC, search engines, etc.) of information
search.
Practical
Introduction to library and its services; Role of libraries in education,
research and technology transfer; Classification systems and organization
of library; Sources of information- Primary Sources, Secondary Sources
and Tertiary Sources; Intricacies of abstracting and indexing services
(Science Citation Index, Biological Abstracts, Chemical Abstracts, CABI
Abstracts, etc.); Tracing information from reference sources; Literature
survey; Citation techniques/Preparation of bibliography; Use of CD-ROM
Databases, Online Public Access Catalogue and other computerized library
services; Use of Internet including search engines and its resources; e-
resources access methods.
PGS 502 TECHNICAL WRITING AND COMMUNICATIONS SKILLS 0+1

Objective
To equip the students/scholars with skills to write dissertations, research
papers, etc.
To equip the students/scholars with skills to communicate and articulate in
English (verbal as well as writing).
Practical
Technical Writing - Various forms of scientific writings- theses, technical
papers, reviews, manuals, etc; Various parts of thesis and research
communications (title page, authorship contents page, preface, introduction,
86
review of literature, material and methods, experimental results and
discussion); Writing of abstracts, summaries, précis, citations etc.;
commonly used abbreviations in the theses and research communications;
illustrations, photographs and drawings with suitable captions; pagination,
numbering of tables and illustrations; Writing of numbers and dates in
scientific write-ups; Editing and proof-reading; Writing of a review article.
Communication Skills - Grammar (Tenses, parts of speech, clauses,
punctuation marks); Error analysis (Common errors); Concord;
Collocation; Phonetic symbols and transcription; Accentual pattern: Weak
forms in connected speech: Participation in group discussion: Facing an
interview; presentation of scientific papers.
Suggested Readings
Chicago Manual of Style. 14th Ed. 1996. Prentice Hall of India.
Collins’ Cobuild English Dictionary. 1995. Harper Collins.
Gordon HM & Walter JA. 1970. Technical Writing. 3rd Ed. Holt, Rinehart
& Winston.
Hornby AS. 2000. Comp. Oxford Advanced Learner’s Dictionary of
Current English. 6th Ed. Oxford University Press.
James HS. 1994. Handbook for Technical Writing. NTC Business Books.
Joseph G. 2000. MLA Handbook for Writers of Research Papers. 5th Ed.
Affiliated East-West Press.
Mohan K. 2005. Speaking English Effectively. MacMillan India.
Richard WS. 1969. Technical Writing. Barnes & Noble.
Robert C. (Ed.). 2005. Spoken English: Flourish Your Language. Abhishek.
Sethi J & Dhamija PV. 2004. Course in Phonetics and Spoken English. 2nd
Ed. Prentice Hall of India.
Wren PC & Martin H. 2006. High School English Grammar and
Composition. S. Chand & Co.
PGS 503 INTELLECTUAL PROPERTY AND ITS 1+0

(e-Course) MANAGEMENT IN AGRICULTURE
Objective
The main objective of this course is to equip students and stakeholders with
knowledge of intellectual property rights (IPR) related protection systems,
their significance and use of IPR as a tool for wealth and value creation in a
knowledge-based economy.
Theory
Historical perspectives and need for the introduction of Intellectual
Property Right regime; TRIPs and various provisions in TRIPS Agreement;
Intellectual Property and Intellectual Property Rights (IPR), benefits of
securing IPRs; Indian Legislations for the protection of various types of
Intellectual Properties; Fundamentals of patents, copyrights, geographical
indications, designs and layout, trade secrets and traditional knowledge,
trademarks, protection of plant varieties and farmers’ rights and bio-
diversity protection; Protectable subject matters, protection in
biotechnology, protection of other biological materials, ownership and
period of protection; National Biodiversity protection initiatives;
Convention on Biological Diversity; International Treaty on Plant Genetic
Resources for Food and Agriculture; Licensing of technologies, Material
87
transfer agreements, Research collaboration Agreement, License
Agreement.
Suggested Readings
Erbisch FH & Maredia K.1998. Intellectual Property Rights in Agricultural
Biotechnology. CABI.
Ganguli P. 2001. Intellectual Property Rights: Unleashing Knowledge
Economy. McGraw-Hill.
Intellectual Property Rights: Key to New Wealth Generation. 2001. NRDC
& Aesthetic Technologies.
Ministry of Agriculture, Government of India. 2004. State of Indian
Farmer. Vol. V. Technology Generation and IPR Issues. Academic
Foundation.
Rothschild M & Scott N. (Ed.). 2003. Intellectual Property Rights in
Animal Breeding and Genetics. CABI.
Saha R. (Ed.). 2006. Intellectual Property Rights in NAM and Other
Developing Countries: A Compendium on Law and Policies. Daya
Publ. House.
The Indian Acts - Patents Act, 1970 and amendments; Design Act, 2000;
Trademarks Act, 1999; The Copyright Act, 1957 and amendments; Layout
Design Act, 2000; PPV and FR Act 2001, and Rules 2003; National
Biological Diversity Act, 2003.
PGS 504 BASIC CONCEPTS IN LABORATORY TECHNIQUES 0+1

Objective
To acquaint the students about the basics of commonly used techniques in
laboratory.
Practical
Safety measures while in Lab; Handling of chemical substances; Use of
burettes, pipettes, measuring cylinders, flasks, separatory funnel,
condensers, micropipettes and vaccupets; washing, drying and sterilization
of glassware; Drying of solvents/chemicals. Weighing and preparation of
solutions of different strengths and their dilution; Handling techniques of
solutions; Preparation of different agro-chemical doses in field and pot
applications; Preparation of solutions of acids; Neutralisation of acid and
bases; Preparation of buffers of different strengths and pH values. Use and
handling of microscope, laminar flow, vacuum pumps, viscometer,
thermometer, magnetic stirrer, micro-ovens, incubators, sandbath,
waterbath, oilbath; Electric wiring and earthing. Preparation of media and
methods of sterilization; Seed viability testing, testing of pollen viability;
Tissue culture of crop plants; Description of flowering plants in botanical
terms in relation to taxonomy
Suggested Readings
Furr AK. 2000. CRC Hand Book of Laboratory Safety. CRC Press.
Gabb MH & Latchem WE. 1968. A Handbook of Laboratory Solutions.
Chemical Publ. Co.
88
PGS 505 AGRICULTURAL RESEARCH, RESEARCH ETHICS 1+0
(e-Course) AND RURAL DEVELOPMENT PROGRAMMES
Objective
To enlighten the students about the organization and functioning of
agricultural research systems at national and international levels, research
ethics, and rural development programmes and policies of Government.
Theory
UNIT I
History of agriculture in brief; Global agricultural research system: need,
scope, opportunities; Role in promoting food security, reducing poverty and
protecting the environment; National Agricultural Research Systems
(NARS) and Regional Agricultural Research Institutions; Consultative
Group on International Agricultural Research (CGIAR): International
Agricultural Research Centres (IARC), partnership with NARS, role as a
partner in the global agricultural research system, strengthening capacities
at national and regional levels; International fellowships for scientific
mobility.
UNIT II
Research ethics: research integrity, research safety in laboratories, welfare
of animals used in research, computer ethics, standards and problems in
research ethics.
UNIT III
Concept and connotations of rural development, rural development policies
and strategies. Rural development programmes: Community Development
Programme, Intensive Agricultural District Programme, Special group –
Area Specific Programme, Integrated Rural Development Programme
(IRDP) Panchayati Raj Institutions, Co-operatives, Voluntary
Agencies/Non-Governmental Organisations. Critical evaluation of rural
development policies and programmes. Constraints in implementation of
rural policies and programmes.
Suggested Readings
Bhalla GS & Singh G. 2001. Indian Agriculture - Four Decades of
Development. Sage Publ.
Punia MS. Manual on International Research and Research Ethics. CCS,
Haryana Agricultural University, Hisar.
Rao BSV. 2007. Rural Development Strategies and Role of Institutions -
Issues, Innovations and Initiatives. Mittal Publ.
Singh K.. 1998. Rural Development - Principles, Policies and
Management. Sage Publ.
PGS 506 DISASTER MANAGEMENT 1+0

(e-Course)
Objective
To introduce learners to the key concepts and practices of natural disaster
management; to equip them to conduct thorough assessment of hazards,
and risks vulnerability; and capacity building.
Theory
UNIT I
Natural Disasters- Meaning and nature of natural disasters, their types and
effects. Floods, Drought, Cyclone, Earthquakes, Landslides, Avalanches,
89
Volcanic eruptions, Heat and cold Waves, Climatic Change: Global
warming, Sea Level rise, Ozone Depletion
UNIT II
Man Made Disasters- Nuclear disasters, chemical disasters, biological
disasters, building fire, coal fire, forest fire. Oil fire, air pollution, water
pollution, deforestation, Industrial wastewater pollution, road accidents, rail
accidents, air accidents, sea accidents.
UNIT III
Disaster Management- Efforts to mitigate natural disasters at national and
global levels. International Strategy for Disaster reduction. Concept of
disaster management, national disaster management framework; financial
arrangements; role of NGOs, Community-based organizations, and media.
Central, State, District and local Administration; Armed forces in Disaster
response; Disaster response: Police and other organizations.
Suggested Readings
Gupta HK. 2003. Disaster Management. Indian National Science
Academy. Orient Blackswan.
Hodgkinson PE & Stewart M. 1991. Coping with Catastrophe: A
Handbook of Disaster Management. Routledge.
Sharma VK. 2001. Disaster Management. National Centre for Disaster
Management, India.
90

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NEW AND RESTRUCTURED

POST-GRADUATE CURRICULA & SYLLABI

Dairy Science & Technology

Executive Summary 3-4

Dairy Technology 9-30

Dairy Microbiology 31-52

Dairy Chemistry 53-67

Dairy Engineering 68-85

Compulsory Non-Credit Courses 86-90

• A new course introduced on “Traditional and value-added dairy products” thereby

• Earlier course “Dairy Processing-III’ restructured into “Membrane technology in

• The doctoral-level course “Product-Process Monitoring” has been revamped with

• Biotechnological applications in dairy processing with special reference to

• New biotechnological tools being essential in implementing the new syllabi,

• Recent advances in the area of chemistry of milk constituents have been

• Newer developments in the area of electronics as applied to industrial

(Constituted by ICAR vide Office order No. F. No. 13 (1)/2007- EQR

Name & Designation Address Specialization

Dr. J.B. Prajapati Dairy Microbiology Division Dairy

Dr. P.S. Prajapati Dairy Technology Division Dairy

On the recommendations of the NCG, the Council constituted 18 Broad Subject

A. The Committee members (Convener, a Member and Member-Secretary) at NDRI

Subject Master’s programme Doctoral programme

CODE COURSE TITLE CREDITS

DT 511 ADVANCED DAIRY PROCESSING 3+1

DT 512 ADVANCED FOOD PROCESSING 3+1

DT 513 RHEOLOGY OF DAIRY & FOOD PRODUCTS 2+1

DT 514 DAIRY PROCESS BIOTECHNOLOGY 2+1

DT 515 TRADITIONAL AND VALUE ADDED PRODUCTS 2+1

DT 521 MEMBRANE TECHNOLOGY IN DAIRY PROCESSING 2+1

DT 522 ADVANCED DAIRY AND FOOD PACKAGING 2+1

DT 523 ALTERNATIVE PROCESSES FOR DAIRY 2+1

DT 524 FUNCTIONAL FOODS AND NEW PRODUCT 3+1

DT 611 ADVANCES IN LIPID TECHNOLOGY 3+0

DT 612 ADVANCES IN PROTEIN TECHNOLOGY 3+0

• American Journal of Clinical Nutrition

• Application of UF retentates in value added products and health foods.

CODE COURSE TITLE CREDITS

DM 512* MICROBIAL PHYSIOLOGY 2+1

DM 513 METHODS IN MICROBIOLOGY 2+2

DM 514 ENVIRONMENTAL MICROBIOLOGY 2+1

DM 515* MICROBIOLOGY OF PROCESSED DAIRY FOODS 3+1

DM 516 APPLICATION OF BIOTECHNOLOGY IN DAIRY INDUSTRY 2+1

DM 521* DAIRY STARTER CULTURES 2+1

DM 522 MICROBIAL GENETICS 2+1

DM 523* MICROBIAL QUALITY AND SAFETY IN DAIRY INDUSTRY 2+2

DM 524 FEED AND RUMEN MICROBIOLOGY 2+1

DM 525 PROBIOTICS AND FERMENTED DAIRY PRODUCTS 2+1

DM 526 MICROBIAL FERMENTATION AND TECHNOLOGY 2+1

DM 591 MASTER’S SEMINAR 1+0

DM 599 MASTER’S RESEARCH 20

DM 611 MICROBIAL DIVERSITY AND PHYSIOLOGY 3+0

DM 612 ADVANCES IN MICROBIAL GENETICS 3+0

DM 621 ADVANCES IN DAIRY AND FOOD MICROBIOLOGY 3+0

DM 622 MICROBIOLOGY OF FOOD-BORNE PATHOGENS 3+0

DM 691 DOCTORAL SEMINAR I 1+0

DM 692 DOCTORAL SEMINAR II 1+0

DM 699 DOCTORAL RESEARCH 45

DM 511 MICROBIAL MORPHOLOGY AND TAXONOMY 2+1

DM 512 MICROBIAL PHYSIOLOGY 2+1