ANALYTICAL CHEMISTRY INTERNATIONAL UNIVERSITY-VN HCM

SCHOOL OF BIOTECHNOLOGY

INTERNATIONAL UNIVERSITY- VNU HCM CITY

SCHOOL OF BIOTECHNOLOGY

EXPERIMENT 1: VOLUME MEASURING TECHNIQUE AND

STATISTIC ANALYSIS

Instructor: MSc. BUI XUAN ANH DAO

GROUP NUMBER: 2
STUDENT’S NAME STUDENT’S ID
TRẦN NGỌC KHÁNH BTBTIU19070
TRẦN HOÀNG CÁT TƯỜNG BTBTIU19137
ĐÕ HUỲNH LAN ANH BTBTIU19053

Distribution:

TASKS STUDENT’S NAME STUDENT’S ID Sign

Introduction and
Trần Ngọc Khánh BTBTIU19070
Conclusion

Result Đỗ Huỳnh Lan Anh BTBTIU19053

Discussion Trần Hoàng Cát Tường BTBTIU19137

1
ANALYTICAL CHEMISTRY INTERNATIONAL UNIVERSITY-VN HCM
SCHOOL OF BIOTECHNOLOGY

I. Introduction
The experiment was set up for students to practice volume measuring
techniques as well as analyze data in chemistry laboratories. In this
experiment, we examine data using both statistical analysis and calibration.
Statistical analysis is a statistical method for analyzing quantitative data.
Since experimentation typically leads to multiple measurements of the same
object being subject to error, it is used to summarize data using average
figures and to quantify the uncertainty in observed variables. By propagating
the measurement error through a mathematical model, statistical analysis
may be utilized to estimate the error in the resulting quantity. The analysis of
variance is another statistical study that is used to find variations between
measurements of two or more samples. In addition, if one variable is
measured in respect to the other, the relationship between the two variables
may be described statistically using regression analysis.
Experience 1: STATISTICAL ANALYSIS

No Mass of object 1(g): Mass of object 2(g):

Green Bean Black Bean

1 0.0721 0.2184

2 0.0666 0.1552

3 0.0612 0.2046

4 0.0594 0.1671

5 0.0603 0.1577

6 0.0669 0.1500

7 0.0538 0.1887

8 0.0562 0.1906

9 0.0524 0.1329

10 0.0600 0.1093

11 0.0620 0.1658

12 0.0796 0.1527

13 0.0682 0.1825

14 0.0635 0.1176

2
ANALYTICAL CHEMISTRY INTERNATIONAL UNIVERSITY-VN HCM
SCHOOL OF BIOTECHNOLOGY

15 0.0527 0.1210

Mean 0.0623 0.1609

Standard deviation 0.0075 0.0322

Table 1: The average masses of two types of beans
Report:
For Object 1 (Green Bean), N1 = 15 For Object 2 (Black Bean), N2 = 15

X1= 0.0623; s1=0.0075 X2=0.1609; s2=0.0322

2 2
( )
𝑠1 𝑁1−1 +𝑠2(𝑁2−1)
Spooled = 𝑁1+𝑁2−2

Null hypothesis Ho: µ1 = μ2

Alternative hypothesis Ha: µ1≠μ2
Degree of freedom: df = N1 + N2 – 2 = 15 + 15 – 2 = 28
Spooled = 0.0234
𝑋1−𝑋2
t=
𝑁1+𝑁2
𝑆𝑝𝑜𝑜𝑙𝑒𝑑 𝑁1𝑁2

⇨ t= -11.540
tcrit at 95% confidence interval and 28 degree of freedom is 2.09
The H0 cannot be rejected since t = -11.540 < t-crit. As a result, the mean of black
beans is the same as the mean of green beans.

Experience 2: Cool water, Warm water, Distilled water

Cool water Warm water Distilled water
Trial 1 9.96 9.87 9.94
Trial 2 9.96 9.80 9.86
Trial 3 9.95 9.83 10.03
Total 29.87 29.50 29.83
Mean 9.96 9.83 9.94
Standard 0.0058 0.0351 0.0850
deviation
Table 2: Pipette calibration at various water temperatures
Confidence intervals:
t value at 95% confidence interval of 2 degree of freedom is 4.30
- Cool water:
4.30*0.0058
95% CI= 9.96 ± = 9.96 ± 0.0144
3
- Warm water:
4.30*0.0351
95% CI= 9.83 ± = 9.83 ± 0.0871
3

3
ANALYTICAL CHEMISTRY INTERNATIONAL UNIVERSITY-VN HCM
SCHOOL OF BIOTECHNOLOGY

- Distilled water:
4.30*0.0850
95% CI= 9.94 ± = 9.94 ± 0.2110
3
Calculate and compare the density (kg/m3) of different solutions:
6
1 kg=10 mg
3 6
1 m =10 mL
6
1 kg=10 mg
3 6
1 m =10 mL
1 kg=106 mg
1 m3=106 mL
- The density of distilled water:
𝑚 µ 9.94
𝑑𝑤 = 𝑣
= 𝑣
= 10
= 0. 994 (𝑘𝑔/𝑚3)
- The density of cool water:
𝑚 µ 9.96
𝑑𝑤 = 𝑣
= 𝑣
= 10
= 0. 996 (𝑘𝑔/𝑚3)
- The density of hot water:
𝑚 µ 9.83
𝑑𝑤 = 𝑣
= 𝑣
= 10
= 0. 983 (𝑘𝑔/𝑚3)

Experience 3: Distilled water

Volumetric Cylinder Pasteur pipette
pipette
Trial 1 9.94 8.66 9.36
Trial 2 9.86 8.63 9.31
Trial 3 10.03 8.97 9.34
Total 29.83 26.26 28.01
Mean 9.94 8.75 9.34
Standard 0.0850 0.1882 0.0252
deviation
Table 3: Calibrate distilled water using a Volumetric pipette, a Cylinder, and a
Pasteur pipette.
Confidence intervals:
t value at 95% confidence interval of 2 degree of freedom is 4.30
- Volumetric pipette:
4.30*0.0850
95% CI= 9.94 ± = 9.94 ± 0.2110
3
- Cylinder:
4.30*0.1882
95% CI= 9.94 ± = 9.94 ± 0.4672
3
- Pasteur pipette:

4
ANALYTICAL CHEMISTRY INTERNATIONAL UNIVERSITY-VN HCM
SCHOOL OF BIOTECHNOLOGY
4.30*0.0252
95% CI= 9.94 ± = 9.94 ± 0.0626
3
Compare the volumetric pipette to the population mean:
t-critical at 95% confidence interval and 2 degree of freedom is 4.30
Null hypothesis: μ = µ0
Alternative hypothesis: μ≠µ0
t-critical at 95% confidence interval and 2 degree of freedom is 4.30
9.94−10
0.0850
𝑡𝑡𝑒𝑠𝑡 = = − 0. 4075
3
𝑡 <− 𝑡𝑐𝑟𝑖𝑡, The null hypothesis is rejected. The experimental value differs significantly
from the known value.
Compare the Pasteur pipette to the population mean:
t-critical at 95% confidence interval and 2 degree of freedom is 4.30
Null hypothesis: µ1 = µ0
Alternative hypothesis: µ1 ≠ µ0
t-critical at 95% confidence interval and 2 degree of freedom is 4.30
9.34−10
0.0252
𝑡𝑡𝑒𝑠𝑡 = = − 15. 1211
3
𝑡 <− 𝑡𝑐𝑟𝑖𝑡, The null hypothesis is rejected. The experimental value differs significantly
from the known value.
Compare the Cylinder to the population mean:
t-critical at 95% confidence interval and 2 degree of freedom is 4.30
Null hypothesis: µ2 = µ0
Alternative hypothesis: µ2 ≠ µ0
t-critical at 95% confidence interval and 2 degree of freedom is 4.30
8.75−10
0.1882
𝑡𝑡𝑒𝑠𝑡 = = − 3. 8347
3
𝑡 <− 𝑡𝑐𝑟𝑖𝑡, The null hypothesis is rejected. The experimental value differs significantly
from the known value.
II. Discussion

Experiment 1: STATISTIC ANALYSIS

Mean of beans have a similar average. Green beans have a higher standard deviation than
black beans. Green beans have a broader confidence interval than black beans.

5
ANALYTICAL CHEMISTRY INTERNATIONAL UNIVERSITY-VN HCM
SCHOOL OF BIOTECHNOLOGY

Experiment 2: Calibration

Based on the findings of the preceding studies, we may conclude that the volumetric pipette
provides more precise results than two other measuring equipment ( Cylinder & Pasteur
pipette ). Furthermore, the table of statistics after each experiment shows that there are errors
and do not give us the same results.

III. Conclusion
Briefly, through the three experiments in lab 1, the accuracy of volumetric glassware
are uncertain.To attain the accuracy results,researchers must apply correct
technique and take measurements at the temperature for which the glassware was
calibrated.