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    ICMR No. SUPRA001f: Real Time PCR

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    Subhash Ranjan
    The laboratory report is for a 22-year-old female, Mrs. Rajni Agarwal, who was tested for COVID-19. The nasopharyngeal and oropharyngeal swab sample collected on July 30th, 2021 tested negative for COVID-19 on real-time PCR testing. Both the N gene and Orf gene Ct values were negative, indicating no detectable SARS-CoV-2 virus. The report provides information on the methodology, interpretation, and limitations of the qualitative COVID-19 PCR test performed.

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    ICMR No. SUPRA001f: Real Time PCR

    Uploaded by

    Subhash Ranjan
    99 views1 page
    The laboratory report is for a 22-year-old female, Mrs. Rajni Agarwal, who was tested for COVID-19. The nasopharyngeal and oropharyngeal swab sample collected on July 30th, 2021 tested negative for COVID-19 on real-time PCR testing. Both the N gene and Orf gene Ct values were negative, indicating no detectable SARS-CoV-2 virus. The report provides information on the methodology, interpretation, and limitations of the qualitative COVID-19 PCR test performed.

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    The laboratory report is for a 22-year-old female, Mrs. Rajni Agarwal, who was tested for COVID-19. The nasopharyngeal and oropharyngeal swab sample collected on July 30th, 2021 tested negative for COVID-19 on real-time PCR testing. Both the N gene and Orf gene Ct values were negative, indicating no detectable SARS-CoV-2 virus. The report provides information on the methodology, interpretation, and limitations of the qualitative COVID-19 PCR test performed.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    99 views1 page

    ICMR No. SUPRA001f: Real Time PCR

    Uploaded by

    Subhash Ranjan
    The laboratory report is for a 22-year-old female, Mrs. Rajni Agarwal, who was tested for COVID-19. The nasopharyngeal and oropharyngeal swab sample collected on July 30th, 2021 tested negative for COVID-19 on real-time PCR testing. Both the N gene and Orf gene Ct values were negative, indicating no detectable SARS-CoV-2 virus. The report provides information on the methodology, interpretation, and limitations of the qualitative COVID-19 PCR test performed.

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    © All Rights Reserved
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    of 1

    LABORATORY REPORT

    Name : Mrs. Rajni Agarwal Sex/Age :Female / 22 Years Case ID : 10405805072


    Ref. By : Dis. At : Pt. ID : 774986
    Bill. Loc. : Pt. Loc :
    Reg Date and Time : 30-Jul-2021 12:03 Sample Type : Nasopharyangeal + Mobile No. : 7014291002
    Oropharyngeal Swab
    Sample Date and Time : 30-Jul-2021 12:03 Sample Coll. By : Ref Id1 :
    Report Date and Time : 31-Jul-2021 02:41 Acc. Remarks Ref Id2 :

    TEST RESULTS UNIT BIOLOGICAL REF RANGE REMARKS


    Genomics
    COVID19 Qualitative by Real time PCR (ICMR No. SUPRA001f)

    COVID19 Interpretation NEGATIVE


    Real time PCR

    N gene (Ct) Negative


    Orf gene (Ct) Negative

    Test: Qualitative test of COVID19 RNA by standard procedure on rt Real-time PCR.


    Methodology: Reverse transcriptase Real-time Polymerase chain reaction.
    Interpretations:
    Cycle threshold (Ct value) Value ranges from 15-40 cycle. Lower the Ct value higher is the viral load (Inversely proportional).
    Kindly correlate with the clinical presentation and findings.
    According to latest CDC guidelines, Ct cutoff of more than 33 is not considered as infective as it is extremely
    difficult to detect any live virus in a sample above the threshold of 33 cycles.
    Clinical Significance:
    a. Coronaviruses are a family of large RNA viruses with size ranging from 26 to 32 kb.
    b. As the coronavirus is anRNA virus it has a relatively high mutation rate resulting in rapid evolution.
    c. In December 2019,a new deadly coronavirus known as 2019-nCoV, which has a high sequence similarity to SARS-CoV, was
    identified and has caused a pneumonia outbreak in Wuhan, China and spread globally.
    Limitations:
    a. The results of this test are highly dependent on the sampling technique employed, sample type, cold-chain maintenance
    andclinical condition. There is poor standardization between commercially available PCR tests, and results from different
    institutions should not be directly compared. Results are best monitored using a single institution.
    b. Presence of PCR inhibitors (cannot be traced by technologist), specimen collected very early/late in infection or viral load lesser
    than the assay lower limit of detection as wellas presence of rare genotypes or mutations may result in false-negative report.
    c. False-positive report may be obtained in cases where there is possibility of background RNA contamination from pre
    analyticalor in lab environment.
    d. The assay performance characteristics for this test are determined by STMPL which is used for clinical diagnosis. This test
    isnot approved by FDA nor accredited by NABL or CAP.
    e. RT-PCR kits used for this assay are approved by ICMR (Supratech Micropath Laboratory & Research Institute Pvt. Ltd. ICMR
    No. SUPRA001f). Test performed on Quantstudio 5 Real-time PCR machine.

    End Of Report

    # For test performed on specimens received or collected from non-NSRL locations, it is presumed that the specimen belongs to the patient named
    or identified as labeled on the container/test request and such verification has been carried out at the point generation of the said specimen by the
    sender. NSRL will be responsible Only for the analytical part of test carried out. All other responsibility will be of referring Laboratory.

    Note:(LL-VeryLow,L-Low,H-High,HH-VeryHigh ,A-Abnormal)

    Page 1 of 1

    Dr. Shiva Murarka Dr. Sandip Shah Printed On: 31-Jul-2021 02:52

    Ph.D. (Scientist) M.D. (Path. & Bact.)


    Consultant Pathologist

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