VPT Agonist
VPT Agonist
VPT Agonist
Placebo is a vehicle for cure by suggestion and is surprisingly often successful though
only temporarily. It can be used as a control in scientific evaluation of drugs and to
benefit or please a patient not by pharmacological actions but by psychological means
(Latin: Placebo – I shall be pleasing or acceptable).
Posology is the study of the medicine dosages, which varies with the species of animals,
the intended effect of the drug and the individual tolerance or susceptibility.
Pro-drugs are drugs that are inactive or have a low order of activity in the form
administered and are metabolised to the active form in the body.
Empirical therapy is the use of certain agents that prove successful in a series of cases
of the same disease, although, it is not possible to explain their actions. Their value has
been demonstrated by experience.
Pharmacokinetics
• Oral route is safer and economical but several drugs are not effective by this
route because of high first pass metabolism in the liver and intestinal wall.
• Sublingual route avoids first pass metabolism as in the IV route, can be used in
emergencies, can be self administered Eg., Nitroglycerine, isosorbide dinitrate,
clonidine, nifedipine etc.
• Inhalational route is the route by which the rate of drug delivery can be
controlled like i.v. infusion. (Eg., Salbutamol for Asthma and inhalational
anaesthetic agents like nitrous oxide)
• Rectal route avoids first pass metabolism to 50% extent. Eg. Diazepam for
unconscious animals
Passage of drug across membrane
✓ Only the nonionized (uncharged) form of a drug crosses biomembranes; When
medium is same, drugs can cross the membrane because of high degree of
non-ionization. Thus, acidic drugs can cross the membranes in acidic medium;
Alkaline drugs can cross the membranes in acidic medium.
Different methods
.
• Passive transfer
✓ Simple diffusion
✓ Filtration
• Specialized transport
✓ Facilitated diffusion
✓ Active transport
✓ Endocytosis / Phagocytosis
First-Pass Effect
✓ With oral administration, drugs are absorbed into the portal circulation and
initially distributed to the liver. For some drugs, their rapid hepatic metabolism
decreases bioavailability, i.e., the “first-pass” effect. Examples include lidocaine (IV
vs. PO) and nitroglycerin (sublingual).
Bioequivalence
✓ Many different pharmaceutical companies can manufacture same compound
(with same dose as well as dosage form).
✓
In general
✓ The more lipid soluble (less polar) a drug is, the better it is absorbed from the
gut and the more widely it is distributed in the body.
✓ However, it is also true that most drug absorption from the gut occurs in the
proximal duodenum.
Distribution
✓ Drugs which are lipid soluble are more likely to cross the blood vessel wall and
thus have high volume of distribution.
✓ If a drug is highly bound to plasma proteins, (e.g., warfarin, benzodiazepines,
furosemide, calcium channel blockers, digitoxin etc.) it will behave like a large
molecule and more likely to stay in the plasma.
Drug reservoirs – Sites other than the site of action can act as reservoirs for the drug if
enough amount of the drug is sequestered there. For example
Metabolism
• Metabolism is the term used to denote normal anabolic and catabolic reactions
of the body.
• Biotransformation is probably the best term useful for describing the biological
fate of foreign compounds. Liver is the most important organ for
biotransformation, but athe lungs, kidney and gastrointestinal epithelium also
play a role.
• In general all of these reactions are grouped into two major categories as Phase I
and Phase II reactions.
Excretion
Tubular reabsorption:
✓ Tubular reabsorption depends on the lipid solubility.
✓ The reabsorption of drug from the lumen of the distal convoluted tubules into
plasma occurs either by simple diffusion or by active transport.
✓ When the urine is acidic, the degree of ionization of basic drug increase and their
reabsorption decreases. Conversely, when the urine is more alkaline, the degree
of ionization of acidic drug increases and the reabsorption decreases.
Tubular secretion:
✓ It does not depend on lipid solubility or plasma protein binding.
✓ Drugs utilizing the sametransporter may show drug interactions e.g. probenecid
decreases the excretion of penicillin.
Kinetics of Elimination
✓ Time to reach steady state depends on t½. It takes approximately 5 half lives.
Pharmacodynamics
✓ Drugs may act by physical mechanism (e.g. osmotic diuretics), chemical action
(e.g.antacids), stimulation or inhibition of enzymes (competitive and non-
competitive inhibition) or via receptors.
Receptors
These are the binding sites of the drug with functional correlate. Two important terms
related to the receptors are affinity and intrinsic activity (IA).
Affinity is the ability of a drug to combine with the receptor. Drugs with high affinity can
be used in low concentrations.
After binding to the receptor, the ability to activate the receptor is called its intrinsic
activity (IE) . It varies from –1 through zero to +1.
Antagonist
These may be physical, chemical, physiological or pharmacological.
✓ Physical antagonist binds to the drug and prevents its absorption like charcoal
binds to the alkaloids and prevents their absorption.
.
Intracellular Receptors
o These types of receptors are slowest acting. These may be present in the
cytoplasm (glucocorticoids, mineralocorticoids, and vit. D) or in the
nucleus (T3, T4, Retinoic acid, PPAR, estrogen, progesterone and
testosterone).
o Both type of receptors finally act by nuclear mechanisms (i.e. by affecting
transcription).
Quantal DRC
When the response is an ‘all or none’ phenomenon (e.g. antiemetic drug stopping
the vomiting or not), the y-axis (response axis) shows the number of person responding
and X-axis shows the plasma concentration. It is used to calculate ED50 and LD50.
Median Effective Dose (ED50): It is the dose that will produce the half of the maximum
(50%) response. More is ED50, lower is the potency and vice a versa.
Median Lethal Dose (LD50): It is the dose that will result in death of 50% of the animals
receiving the drug. More is LD50, safer is the drug.
Graded DRC
When the response can be graded (e.g. reduction in BP), the y-axis shows the magnitude
ofresponse.DRC is usually hyperbola in shape. As curved lines cannot give good
mathematical comparisons, so usually the dose is converted to log dose to form log
DRC, which gives a sigmoid shaped curve.
Three important parameters (potency, efficacy and slope of curve) can be determined
from DRC.
Potency
It is the measure of the amount of a drug needed to produce the response. Drugs
producing the same response at lower dose are more potent whereas those requiring large
dose are less potent.In DRC, more a drug is on left side of the graph, higher is its potency
and vice a versa. In Fig,drug A is more potent than drug B.
Slope
If the DRC is steeper, that means the response will increase dramatically with
slight increase in dose. Thus, drugs having steeper DRC have narrow therapeutic index
(like barbiturates) than those having less steep curves (e.g. benzodiazepines).
Therapeutic Index = LD50/ED50
Standard safety margin = (LD1 − ED99 / ED99) X100
• Morphine (a μ-opoid agonist) is more potent in cats than dogs. In dogs, the
dose is 1 mg/kg where it consistently produces sedation. In cats, the dose for
analgesia is 0.1 mg/kg. Higher doses in cats may produce excitement.
• Cats have a low level of glucuronyl transferase so that the t1/2 of many
drugs that are conjugated to glucuronide by the liver is much longer. The
classic example is aspirin where the t1/2 in cats is 25–35 hours compared to 8
hours in dogs and 1 hour in horses.
Mechanism of Action:
All LAs are weak bases. These drugs act by penetrating the axonal
membrane (in unionized form) and blocking the voltage gated sodium
channels from within (in ionized form).
Types:
Local anaesthetics (LAs) can be classified into amide and ester types.
Amides are usually long acting and chances of allergic reactions are less whereas
esters are short acting (due to metabolism by esterases present in the plasma).
Ester LAs can cause allergic reactions and also antagonize the action of
sulfonamides due to degradation of PABA.
✓ Lignocaine ✓ Cocaine
✓ Prilocaine ✓ Procaine
✓ Bupivacaine ( Second longest) ✓ Chlorprocaine (Shortest acting)
✓ Dibucaine (Longest acting) ✓ Tetracaine (Amethocaine)
✓ Mepivacaine ✓ Benzocaine
✓ Etidocaine
✓ Ropivacaine
(If the name of the local anaesthetic contains the 2 “i” in the name then it is an
amide type local anaesthetic and if only one “i” it is an ester type of drug)
GENERAL ANAESTHETICS
The stages of anaesthesia are divided into distinct packages, each correlating with a
particular set of physiological responses or reflexes. The organisational scheme includes
four stages of anaesthesia.
Stage I This stage is also known as the stage of analgesia or stage of voluntary
excitement or stage of analgesia and amnesia.
Stage II This stage is also known as the stage of delirium or stage of involuntary
excitement or stage of uninhibited action.
Stage III This stage is also known as the stage of surgical anaesthesia. It is divided
into four planes as Plane 1, Plane 2, Plane 3 and Plane 4.
Plane 1 – Light surgical anaesthesia – roving eye balls and plane ends with
eyes becoming fixed
Plane 3 – Deep surgical anaesthesia – Pupil starts dilating and light reflex is
lost
Preanaesthetic medication
1. The rate at which anaesthesia can be induced and wakefulness resumed focuses
attention on short term biochemical events
2. Wide variation in the structure of anaesthetic drugs
3. Ability of anaesthetics to cause superimposed selective and specific anaesthetic
side effects such as reductions in myocardial contractility.
Theories of Anaesthesia:
Colloidal theory: This theory states that, colloids of nerve protoplasm form a loose
compound with the anaesthetic.
Surface tension theory: According to this theory, a reduction in the surface tension of
neuronal membrane is brought about by the anaesthetics.
Pauling and Miller theory: Pauling and Miller theory states that microcrystals
(clatharates) are formed by the anaesthetic vapour. These in turn reduce the
conductance.
Reduced energy formation theory: This theory states that general anaesthetics depress
the formation of high energy bonds.
Cell membrane expansion theory: This theory states that inhalation anaesthetics are
hydrophobic and therefore distribute to sites in which they are removed from aqueous
environment and because of the close correlation between potency and lipophilicity, it is
theorized that these anaesthetics act on the cell membrane lipid layer.
Properties:
• Nitrous oxide is a gas with maximum MAC and thus least potency. Its MAC is
104% i.e. even with pure (100%) nitric oxide alone, we cannot get complete
anaesthesia. This is thus, not a complete anaesthetic agent.
• Desflurane is the fastest acting agent as it has minimum blood gas partition
coefficient.
• Ether also has a very high value of this coefficient; therefore it is also a slow
acting agent. Due to its slow onset of action, we can differentiate the four
stages of general anaesthesia whereas with modern anaesthetics like
desflurane, these stages are hardly discernible.
Drugs used as inhalation anaesthetics are classified as gases and volatile liquids.
Based on their use, they are also calssified as follows.
Halothane: Is the most widely used agent, highly lipid soluble, potent. It causes
arrhythmia, hangover and the risk of liver damage is high if used repeatedly.
Nitrous oxide: Oderless and colourless gas. It is rapid in action and also an effective
analgesic agent. Its potency is low, hence must be combined with other agents. It is a
relatively free of serious unwanted effects (Second gas effect)
Sevoflurane: has a low blood:gas partition coefficient; therefore, induction and recovery
are rapid.
INTRAVENOUS ANESTHETICS
Barbiturates
In USA ‘al’ is substituted in the name of barbiturates for ‘one’ (For example in
USA thiopentone is known as thiopental).
Classification of barbiturates
Pentobarbital (Nembutal) – This was the most widely used anaesthetic in small animal
surgery. After the introduction of methoxyflurane and halothane its use declined.
• Opium is the air-dried milky exudates obtained from the incised unripe seed
capsules of the poppy plant Papaver somniferum. Opioid analgesics can be used
for general anesthesia, in patients undergoing cardiac surgery and fentanyl and
its derivates are commonly used for these purposes.
• The pharmacological effects result from interactions with one or more of the four
opioid receptors (mu, sigma, kappa and delta).
Dissociative anaesthetics
The drugs that cause the patient to feel dissociated from or unaware of the
surroundings during induction are called dissociative anaesthetics. Dissociative
anaeasthetics depress the cerebral cortex before causing medullary depression.
Chloralose – This drug is metabolised to chloral hydrate and chloral and this chloral
hydrate is in turn metabolised to trichloroethanol. Hypnosis and anaesthesia produced
by chloral hydrate and chloralose are similar.
Concentration-dependent killing
Time-dependent killing
Additive or indifferent effect – the combined effect of the drugs equals the sum of
their independent activities measured separately
Synergistic effect – the combined effects are significantly greater than the independent
effects
Antagonistic effect – the combined effects are significantly less than their independent
effects.
Drug resistance
Antimicrobial susceptibility testing: Agar disc diffusion method, Kirby Bauer method
✓ Bactericidal effect is one that when initiated almost invariably results in cell
death. Cidal drugs preferred in immunocompromised patients and in serious, life
threatening infections.
• Two bactericidal agents are synergistic if the organism is sensitive to both e.g.
isoniazid and rifampicin in tuberculosis.
4. Aminoglycosides
1. PENICILLINS
• Amidase cleavage of the amide bond side chain yields the 6-amino-
penicillanic acid (6-APA) nucleus used in producing semisynthetic
penicillins.
Mechanism of action: Penicillins bind to and inhibit the transpeptidase involved in the
cross-linking of the bacterial cell wall, the third and final step in cell-wall synthesis
3. Broad-spectrum penicillins
a. Aminopenicillins. Ampicillin and amoxicillin are active against many Gram(–) aerobes
(E. coli, Proteus, Haemophilus spp.) as well as Gram(+) pathogens. They are used in all
species for the treatment of susceptible infections. They are acid stable but are not
penicillinase stable. GI absorption of amoxicillin is better than ampicillin.
b. Anti-pseudomonal penicillins
Administration:
✓ Penicillins are generally administered IM. The acid-stable penicillins are
administered orally 2–3 times a day.
✓ Procaine penicillin G is slowly absorbed from IM sites and may provide
therapeutic levels for 24 hours with a single dose.
✓ Benzathine penicillin G is even more slowly absorbed over 48–72 hours but blood
levels attained are relatively low.
Adverse effects.
✓ Allergic reactions to penicillin may occur in animals, especially cattle.
✓ Procaine salts of penicillin should not be used in birds, snakes, turtles, guinea
pigs, or chinchillas because these species are sensitive to procaine.
✓ Procaine penicillin G should not be used in race horses 30 days before racing.
2. CEPHALOSPORINS
3. CARBAPENAMS
• Meropenem is a more recent derivative that is more DHP-1 stable that does not
need cilastatin to inhibit kidney metabolism.
• Aztreonem is used replace aminoglycosides, which are more toxic when used
with macrolides and lincosamides.
5. VANCOMYCIN
• Vancomycin blocks the second step of bacterial cell wall synthesis by inhibiting
polymer release from the cell membrane via bactoprenol by hindering (Second
step of cell wall synthesis ) the transglycosylation reactions (and indirectly
preventing transpeptidation). It is bactericidal for Gram(+) organisms.
SULPHONAMIDES
• Synthetic organic chemicals. - first chemotherapeutic agents used
systemically for prevention and treatment bacterial infections - first
discovered from prontosil dye by Domagk in 1935.
Classification
I. Systemically acting Sulphonamides
1. Short acting sulphonamides (duration <12 hours): sulphadiazine,
sulphafurazole, sulphameraaine,.sulphachlorpyridazine, sulphathiazole and
sulphamlamlde.
2. Intermediate acting sulphonamides (duration 12-24 hours): sulphadimidine,
sulphamethoxazole, sulphamoxole and sulphaphenazole.
3. Long-acting sulphonamides (duration 24 48 hours): sulphadimethoxine,
sulphaethoxypyridazine, sulphamethoxypyridazine and sulphabromomethazine.
Therapeutic uses:
• Sulfamethazine is used in cattle, sheep, and swine. It is slowly excreted and
therapeutic levels are maintained in plasma for 24 hours with a single dose.
• Sulfadimethoxine is a long-acting sulfonamide. It is more soluble and less
toxic than sulfamethazine. The plasma t 1/2 is 10–15 hours.
• Sulfachlorpyridazine is a rapidly absorbed and rapidly excreted sulfonamide
used orally in calves under 1 month of age and in swine for the treatment of
respiratory and enteric infections, especially colibacillosis. Peak levels occur in
1–2 hours in nonruminants and in preruminant calves. The plasma t 1/2 is 1.2
hours.
Adverse effects
• Renal crystalluria due to precipitation of sulfonamides in neutral or acidic urine
may occur with large or prolonged doses or inadequate water intake, especially
with the older, less soluble sulfonamides such as sulfathiazole.
• Therapeutic regimens generally do not extend beyond 5 days and renal
crystalluria is rare.
• Keratoconjunctivitis sicca (KCS) may be observed in dogs treated with
sulfonamides, such as sulfadiazine
• Hypoprothrombinemia, thrombocytopenia, and anemia occur rarely and are
probably immune-mediated reactions.
• Sulfonamides should not be used in animals with preexisting bleeding disorders.
FLUROQUINOLONES
Classification:
• First-generation quinolones: Nalidixic acid, oxolinic acid, flumequine, cinoxacin,
pipemidic acid, piromidic acid and rosoxacin
• Second-generation quinolones: Enrofloxacin, ciprofloxacin, danofloxacin,
marbofloxacin, norfloxacin, ofloxacin, pefloxacin, sarafloxacin
• Third-generation quinolones: levofloxacin, sparfloxacin,
• Fourth-generation quinolones: Moxifloxacin, gatifloxacin, besifloxacin,
clinafloxacin, garenofloxacin, gemifloxacin, sitafloxacin, trovafloxacin and
prulifloxacin
Therapeutic uses
• Enrofloxacin is used in the treatment of dermal, respiratory, and urinary tract
infections (including prostatitis) in dogs, cats, and birds and in respiratory
infections in cattle.
Clinical uses:
• infections of skin, urinary tract and soft tissues in dogs and cats
• Respiratory disease in cattle, enzootic pneumonia in pigs
• Recommended in infections in exotic species
• Dose: 2.5 to 5 mg/kg PO- to most of the species
Moxifloxacin
• Spectrum against – Gr+ve, Gr-ve and anaerobes
Adverse effects.
• Erosion of articular cartilage in young dogs and foals, particularly, if they are
used at high doses for longer than 14 days in rapid growth phase.
• Phototoxicity - Retinal degeneration has been reported due to acute and diffuse
retinal damage in cats.
• Very polar and basic in character. These are not absorbed orally and do not cross
blood brain barrier.
Therapeutic uses.
The aminoglycosides are used in the treatment of Gram(–) infections in all species.
• Neomycin is used orally for the treatment of enteric infections and topically for
treating skin, ear, and eye infections. Topically neomycin sulfate is used as
solution or ointment for dressing wounds and eye infections
• Should not be used with other ototoxic or nephrotoxic drugs such as furosemide
or amphotericin B.
TETRACYCLINES
• The tetracyclines are polycyclic compounds that are amphoteric and that
fluoresce when exposed to ultraviolet light. Most are prepared as the
hydrochloride salt.
• They form insoluble chelates with cations such as Ca2+, Mg2+, Fe3+, and Al3+
and They accumulate in growing teeth and bones.
• A recent derivative of the glycylcyclines, tigecycline, has been developed that has
an effect against methicillin-resistant S. aureus (MRSA).
CHLORAMPHENICOL
MACROLIDES
Uses
• Chlamydial infections
• Mycoplasma and Legionella
Adverse effects:
• Cholestatic jaundice
• Gastro intestinal motilin agonist – diarrhea (Erythromycin have prokinetic
activity also)
• Interactions : Erythromycin inhibits CYP-450; Azithromycin not.
• Not a macrolide, but has the same mechanisms of action and resistance.
• Major adverse effect is resp. for pseudomembranus colitis (by Cl. difficile) and
Super-infection.
METRONIDAZOLE
• They complex with Na+ in the cell membrane to produce passive extracellular
transport of K+
and intracellular influx of H+, which kills bacteria and coccidian by lowering
intracellular pH.
Polymixins are products of Bacillis polymyxa. Originally these antibiotics are used for
their action against Pseudomonas aeuroginosa.
ANTIFUNGAL AGENTS
• Systemic Drugs for Superficial Fungal Infections: Griseofulvin (for ring worm
infection), Allylamines, Ketoconazole, fluconazole and itraconazole
• Topical: Terbinafine, Benzoic acid (6%) with salicylic acid (3%) - Whitfield’s
ointment
ANTIVIRAL DRUGS
Anti herpes virus drugs - Acyclovir is a guanosine derivative with selectivity for
particular herpes.
Anti HIV virus drug - Zidovudine is frequently used NRTI in the treatment of HIV
infections. It can also be used for the prophylaxis of needle stick injury patients and for
the prevention of vertical transmission of HIV from mother to fetus.
ANITI-CANCER DRUGS
• Rapidly multiplying and growing cells are most susceptible to drug effects. These
include the normal cells of the hair follicles, gastrointestinal tract, and bone
marrow.
• Thus, hair loss, vomiting and diarrhea, and leucopenia and thrombocytopenia are
common side effects of therapy
K is constant with value of 10.0 and 10.1 for cats and dogs respectively
Vincristine
✓ Vincristine is the most commonly used mitotic inhibitor in veterinary medicine. It
is employed in the treatment of lymphoreticular neoplasms, carcinomas, and
sarcomas in dogs and cats and transmissible venereal tumors (TVT) in dogs.
• Inhibitors of glycolysis
o Clorsulon and thiacetarsamide.
3. Disruption of tegument
Affecting permeability of cells and vacuolation of tegument - Praziquantel
Vinca rosea
Pharmacological Anticancer drug – common used for the treatment of transveneral tumour
action in dogs.
Withania somnifera
Laptadenia reticulate
Pharmacological Galactagogue
action
Gingiber officinalis
Ricinus communis
Pharmacological Purgative
action
• Paracelsus is often quoted for his statement ‘All substances are poisons; there is
none which is not a poison. The right dose differentiates a poison and a remedy’.
• The amount of toxicant in food and water, which can be consumed daily over a
life time without any significant health risk, is known as ACCEPTABLE DAILY
INTAKE (ADI).
• LD refers to the ‘lowest dose’ of the toxicant administered directly to the animal
in any route. LC refers to the ‘lowest concentration’ of toxicant present in feed
and water. (In LD, the compound is administered directly to the animal on mg/kg
bd wt basis, whereas, in LC the toxicant is present in feed or water (mg/kg of feed
or Liter of water). (LD > LC)
• NOEL (No observed effect level) is the highest dose, which will not produce any
effect, whereas, NOAEL (No observed adverse effect level) is the highest
concentration, which will not produce any adverse effect. (NOAEL > NOEL)
LD > LC > MTD > NOAEL > NOEL > ADI > MRL
• DDT (Dichloro diphenyl trichloro ethane), which is used to control malaria and
typhus was discovered by PAUL MULLER.
Poisons - Classification
4. Poisons, which affect the nerve cells and fibres e.g. Hypnotics,
narcotics, anesthetics, alcohol, some alkaloids and glycosides.
• The phenomenon in which toxic substances elicits beneficial effects at low doses
is known as HORMESIS.
• In oral route of exposure, and liver are responsible for elimination prior to entry
into circulation and hence Pre-systemic elimination is possible
• The common process involved in the absorption of xenobiotics across the cell
membrane is PASSIVE DIFFUSION.
• Arsenic tends to accumulate in HAIR and SKIN (organ of the body). (Hence,
bacterial decomposition of carcass is absent resulting in preserved carcass)
• Rodents are preferred for oral toxicity testing as they lack VOMITION reflex.
• The species of animal that is resistant to the toxic effects of consuming Atropa
belladonna (Belladonna) leaves is RABBIT. (Rabbits contain the enzyme
atropinase, which destroys atropine).
• The breed of dog that is more susceptible to the toxic effects of ivermectin is
COOLIE BREED. (In coolies, ivermectin easily crosses blood brain barrier and
causes neurological symptoms).
• The most common feed contaminant that can be expected during improper
storage is MYCOTOXINS (Aflatoxin).
• The time versus concentration curve of toxicant in the body is BELL OR INVERTED
‘U’ shaped.
• The most commonly used adsorbing agent for binding toxicants in GIT is
ACTIVATED CHARCOAL.
• The type of diuretics or purgatives that are preferred in cases of poisoning are
OSMOTIC/SALINE TYPE. However, for prompt action, furosemide, which is a loop
diuretic is also used.
• The mechanism involved in the enhanced elimination of acidic agents in alkalized
urine and basic agents in acidified urine is ION TRAPPING. (Ion trapping for basic
drugs occurs in the acidic pH of rumen - due to the production of volatile fatty
acids).
• The agents that are used during CNS depression and respiratory arrest are called
as ANALEPTICS. (Eg. Doxapram).
Chelating agents:
Chelating agents are used in the treatment of poisoning with heavy metals. They
incorporate the metal ion into an inner ring structure in the molecule by means of
chemical groups called ligands (Greek word chele = claw, Latin word ligare = to bind).
METALS
• Lead is ubiquitous in nature .Most of the animals live close to ground level and
hence gets more exposure.
• In Pica condition, animals tends to lick walls, chew on dry peelings of paint, eat
wall posters etc. Since, paints are lead based, the animals are affected with lead
poisoning.
• The species that are most susceptible to lead poisoning are DOG, CATTLE and
HORSES.
• The species that is considered as indicator for lead in the environment is DOG,
Since dogs live close to soil are have the habit of frequent digging of soil.
• The species that is very resistant to lead poisoning is SWINE.
• As >90% of ingested lead is eliminated from GIT without absorption and even
after absorption, as only <1% of lead is in free form. Hence, acute lead toxicosis is
not common.
• The organ that is considered as sink for lead is BONE (95% of body’s lead burden
is found in bone).
• Lead toxicity is
✓ result of binding with –SH groups of proteins and enzymes.
✓ decreases ‘haeme’ synthesis through the inhibition of the enzyme δ-
AMINO LEVULINIC ACID SYNTHETASE (ALA-D synthase), Haem synthatase
etc.,
• lead poisoning, basophilic stipplings (BS) are commonly seen in DOGS.
• Lesions are MICROCYTIC HYPOCHROMIC ANAEMIA, BLUE LINES IN GUM
• The characteristic histological picture of lead poisoning in tubular cells of kidney
is INTRANUCLEAR INCLUSION BODIES. (Eosinophilic)
• Neurological symptoms accompanied by GIT symptoms possibly indicate LEAD
poisoning.
Mercury (Hydrargyrism)
ARSENIC
• Arsenites (As3+ or trivalent) are 5-10 times more toxic than Arsenates (As5+ or
pentavalent) due to higher solubility.
• Most toxic gaseous form of arsenic which is released during charging of storage
batteries is ARSINE.
Copper Vs Molybdenum
• Hence, when the animal is associated with copper deficiency, the same animal
always having the chances to be in Molybdenum Toxicity condition. (Vice Versa)
Copper
• The breed of dog that is highly susceptible to copper toxicosis due to genetic
predisposition is BEDLINGTON TERRIER. (Autosomal recessive gene causes
copper retention in liver as a result of failure of excretion).
• In copper poisoning, elevation of liver marker enzymes (AST, SDH, and LDH)
occurs prior to HEMOLYTIC CRISIS (about 3-6 weeks before). Hence, though
marker enzymes are elevated, the level of Cu in blood is not increased until a day
or two before development of hemolysis.
IRON POISONING
• Toxicity is usually noticed in pigs. Toxicity is more in piglets born to selenium and
vitamin E deficient pigs.
• In baby pigs pale skin, corrosion of mucosa, dark faeces, diarrhea and
tachycardia.
• Yellowinsh brown discolouration and oedema of the tissues especially near the
injection site.
• In horses, loss of hair from the mane is the primary symptom of SELENIUM
poisoning.
Common salt
• The most susceptible species for salt poisoning are POULTRY and PIGS. (Poultry
have poor sense of taste and indiscriminate feeding behavior).
• There is no specific treatment. Slat free fresh water must be given initially in small
quantities at more frequent intervals. Isotonic or hypertonic saline
intraperitoneally.
Urea
• Urea is permitted in the diets of ruminants and horses due to the presence of
UREASE microbial enzyme in rumen and caecum respectively.
• Calf age group (Young ruminants) is more resistant to urea toxicity, since rumen
microbial is not well developed.
• The recommended ratio of urea to molasses for straws and other high fiber diets
is 1:5.
Fluoride
• The level of fluoride in drinking water that can cause fluorosis in animals is >2
PPM.
• Fluoride accumulates in BONE and TEETH in the body. (Bone acts as sink for
fluoride similar to lead. However, accumulation of fluoride in teeth occurs only
during formative stages i.e., young age only).
• White and yellow (most toxic) phosphorus are soluble and readily absorbed.
Hence cause toxicity. Whereas red phosphorus is insoluble, hence is non-toxic.
• Excess feeding of wheat bran rich in phosphorus causes BRAN DISEASE in horses.
PHYTOTOXINS
• Toxicity of nitrate is due to its conversion into NITRITE by rumen micro flora.
(Nitrites [NO2-] are 10 times more toxic than nitrates [NO3-]).
• The species that are more susceptible to nitrate poisoning are RUMINANTS.
Cyanide
• Plant enzyme responsible for the release of hydrocyanic acid from cyanogenic
glycosides is β-GLYCOSIDASE. (β-glycosidase is present in mesochymal cells).
• Young and immature plants, plants growing rapidly after drought, wittled
and frost bitten plants are more toxic.
• Drying the plant or making silage reduces the toxic potential of the plant.
Metabolism
• The metabolite of cyanide, which is excreted through urine is
THIOCYANATE.
• The common site for urinary obstruction in bulls and rams due to oxalate stones
is SIGMOID FLEXURE. (In rams, the oxalate crystals also deposit in urethral
process).
• The toxic principle in the seeds of Abrus precatorius is ABRIN and Castor is Ricin -
a type of toxalbumin /lectin (inhibit protein synthesis)
• The species that is more susceptible to abrin and ricin poisoning is HORSE.
• The species that is resistant to Atropa belladonna is RABBIT. (Rabbits contains the
enzyme atropinase enzyme, which destroys atropine)
Cotton seed
• Ruminants are not usually affected. Young calves, swine and poultry are affected.
• Gossypol binds with IRON mineral inhibiting heame synthesis and causing
anemia.
PHOTOSENSITIZATION
TOXICOLOGY OF AGROCHEMICALS
• OC insecticides are not degradable and are persistent in the environment. And
due to high lipid solubility, they accumulate in the food chain and enter human
and animal bodies. Hence, OC compounds are being discouraged.
• The only OC insecticide that does not accumulate in adipose tissue and bio-
degradable by microbes is ENDOSULFAN.
• The species of animal that is highly sensitive for delayed neuropathy caused by
OP compounds (OPIDN) is CHICKEN. (Chicken brain has higher specific activity of
NTE, hence is more susceptible).
• The agents, which are used to reactivate AChE inhibited by OP compounds are
called OXIME REACTIVATORS (Eg. 2-PAM – Pralidoxamine).
Carbamates
• Carbamates bind with AChE at the both sites., Anionic and Esteratic.
• Type II pyrethroids contain α-CYANO group in their structure and are more
active and toxic. (Eg. Deltamethrin, Cypermenthrin, Fenvalarate etc - CS
SYNDROME (Choreoathetosis and Salivation).
• The roots of plants belonging to Derris genus are the source of natural insecticide
ROTENONE. Rotenone is highly toxic for FISH species.
RODENTICIDES
• Toxicity of Zinc phosphide (Zn3P2) is due to the release of PHOSPHINE (PH3) gas.
• The characteristic smell of phosphine gas, which can be used for diagnosis of zinc
phosphide toxicity, is FISH LIKE or ACETYLENE.
Anticoagulant rodenticides
• Warfarin inhibits clotting factors that are dependent on VITAMIN K for synthesis.
(Vitamin K dependent clotting factors are II (Prothrombin), VII, IX and X).
Fluoroacetate
• Fluoroacetate lowers energy production in the body by inhibiting TCA or KREBS
CYCLE.
• Fluoroacetate develops toxicity after a lag period. Hence, the rodent forgets the
exposure. Hence, Fluoroacetate rodenticide is devoid of bait shyness.
• The prominent symptoms in fluoroacetate poisoning are NEUROLOGICAL.
VITAMIN D (cholecalciferol)
• The mechanism of toxicity of vitamin D compounds (cholecalciferol) rodenticides
through the induction of HYPERCALCEMIA.
• Hypercalcemia causes calcification of visceral organs like kidney, blood vessels,
heart and lungs.
• Treatment for vitamin D rodenticide poisoning is CORTICOSTEROIDS and
CALCITONIN.
• Bait shyness and tolerance develops very rapidly for ANTU rodenticide.
Red squill
• Red squill, which is used as a rodenticide is obtained from the plant URGENIA
MARITIMA. (In south India, toxicity due to Indian squill (Urgenia indica) is more
common)
• Toxic glycosidic principle present in red squill is SCILLIROSIDE.
• The most potent neurotoxin in the venom of black widow spider (Lactrodectus
mactans) is α-LACROTOXIN.
• The most susceptible species for tick paralysis is DOG and the type of paralysis
seen induced by ticks is ASCENDING FLACCID.
• The type of toxins present in the venom of elapidae snakes (Cobra, Krait, Mamba,
Coral snakes) are NEUROTOXINS.
• The type of toxins present in the venom of viperidae snakes (Viper, Rattle snake,
Adder) are HAEMOTOXINS.
• The most potent among all marine toxins is TETRADOTOXIN (TTX). In snakes, the
venomous glands are homologues to PAROTID glands in other animals.
MYCOTOXINS
• Molds generally grow in stored fed stuffs containing a moisture content more
than 15%. (The other factors being a relative humidity of >85% and optimum
temperature of25-30oC i.e., without refrigeration.
Rubratoxin
Ochratoxin
• The most susceptible species for ochratoxicosis are BIRDS and PIG.
Zearalenone
Trichothecene
Ergotoxins
BACTERIAL TOXINS
• The ionizing radiation that has least penetrating capacity is ALPHA (α)-
PARTICLES.
• The ionizing radiation that has highest penetrating capacity is GAMMA (γ) RAYS.
• The SI unit of radiation is GRAY (Gy) and the CGS unit is RAD (rad).
• Antibiotics approved for use as growth promoters in food producing animals are
IONOPHORE ANTIBIOTICS. (Monensin, lasalocid and salinomycin are the
commonly used ionophore antibiotics). The most sensitive species for ionophores
toxicity is HORSE – Cardiomyopathy
• The species that is highly sensitive for polychlorinated biphenyls (PCB) toxicity is
MINK.
INDICATION ANTIDOTE
Acetaminophen (Paracetamol) poisoning N-Acetylcysteine
Copper poisoning D – Penicillamine;
Ammonium molybdate
and tetrathiomolybdate
Reversal agent for Yohimbine, Atipamezole HCl
medetomidine, xylazine, and
potentially amitraz
Organophosphate poisoning Atropine sulfate, 2 PAM
Antemortem
Blood 5-10 ml Useful for detecting exposure to most metals, trace elements, cholinesterase, pesticides and
ethylene glycol and also for evaluating erythrocyte and leucocyte morphology
Serum 5-10 ml Useful for evaluation of electrolytes, urea nitrogen, ammonia nitrogen and organ function;
exposure to metals, drugs and vitamins. Serum should be removed from clotted blood, taking
care to avoid hemolysis
Urine 50 ml Useful for detecting exposure to alkaloids, metals, electrolytes, antibiotics, drugs,
sulphonamides and oxalates
Faeces 250 g Useful for detecting recent oral exposures or drugs or toxicants excreted primarily in bile
Vomitus 250 g Useful for detecting ingested poisons of all types, especially those that cannot be measured in
tissue ( e.g. organophosphorous compounds, ionophores)
Hair 5-10 g Useful for detecting dermal exposure to pesticides, chronic accumulation of some metals like
arsenic, selenium.
Postmortem
Liver 100 g Major oragn of biotransformation. Accumulates metals, pesticides, alkaloids, phenols and
some mycotoxins. Bile may be usesul for detecting toxicants concentrated by bile like lead.
Usually frozen till analysis
Kidney 100 g Major organ of excretion for antibiotics and other drugs, metabolized toxicants, alkaloids,
herbicides, some metals, phenolic compounds and oxalates
Rumen 500 g Confirmation of recent oral toxicant exposure. Rumen may degrade some toxicants like
contents nitrates and mycotoxins. Quantitative analysis is difficult as a result of variability of
concentrations and lack of correlation with toxic levels in the tissues. Samples should be
collected from several locations in the rumen and kept frozen till analysis.
Fat 250 g Useful for detection of cumulative fat-soluble toxicants like chlorinated pesticides and dioxins
Ocular fluids Entire Useful for evaluating electrolytes like sodium, calcium, potassium and magnesium, ammonia
eye nitrogen, nitrates and urea nitrogen. Ocular potassium and urea have been used to estimate
time since death. Both aqueous and vitreous humor are useful, but should be collected
separately
Brain Entire Useful for detecting some neurotoxins like chlorinated pesticides, pyrethrins, sodium, mercury.
Environmental
Feeds 2 kg Multiple representative samples should be taken and then either combined and mixed for a
composite sample or retained as individual samples to detect variability in the source or both
Forages (e.g. 5 kg Samples should be taken from multiple locations in a pasture or storage facility using a forage
pasture, sampler for baled hay or stacks. Silage should be frozen to prevent mold and deterioration
hay,silage)
Baits All Entire suspected bait and label should be submitted if available
Water 0.5 – 1 L Useful for detection of nitrates, sulphates, total solids, metals, algae and pesticides. Water
should be allowed to run to clear pipes before collecting from well or storage tank. Bodies of
water from the probable site of exposure should be sampled. Water should be kept
refrigerated until analysis.
Soil /mud/ 1 kg Soil should be collected from root zone depth if plant contamination is suspected. Sampling
sediment from multiple sites may be appropriate. A soil scientist or agronomist may be contacted, if
possible.
REFERENCES