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    Hba1c Wo Lysis A25 2012 11 28

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    Eng-Hussein Mohamed
    This document provides instructions for using the Biosystems A25 Hemoglobin A1c Direct immunoturbidimetric latex test. It includes information on the reagents required, sample preparation by hemolysis, and suggested instrument settings for calibration, procedure, and options when running the test without an onboard lyse application. The test uses two reagents and a hemolysis reagent to prepare hemolyzed samples from whole blood for measurement on a clinical chemistry analyzer.

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    Hba1c Wo Lysis A25 2012 11 28

    Uploaded by

    Eng-Hussein Mohamed
    40 views1 page
    This document provides instructions for using the Biosystems A25 Hemoglobin A1c Direct immunoturbidimetric latex test. It includes information on the reagents required, sample preparation by hemolysis, and suggested instrument settings for calibration, procedure, and options when running the test without an onboard lyse application. The test uses two reagents and a hemolysis reagent to prepare hemolyzed samples from whole blood for measurement on a clinical chemistry analyzer.

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    hba1c_wo_lysis_a25_2012_11_28_

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    This document provides instructions for using the Biosystems A25 Hemoglobin A1c Direct immunoturbidimetric latex test. It includes information on the reagents required, sample preparation by hemolysis, and suggested instrument settings for calibration, procedure, and options when running the test without an onboard lyse application. The test uses two reagents and a hemolysis reagent to prepare hemolyzed samples from whole blood for measurement on a clinical chemistry analyzer.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    40 views1 page

    Hba1c Wo Lysis A25 2012 11 28

    Uploaded by

    Eng-Hussein Mohamed
    This document provides instructions for using the Biosystems A25 Hemoglobin A1c Direct immunoturbidimetric latex test. It includes information on the reagents required, sample preparation by hemolysis, and suggested instrument settings for calibration, procedure, and options when running the test without an onboard lyse application. The test uses two reagents and a hemolysis reagent to prepare hemolyzed samples from whole blood for measurement on a clinical chemistry analyzer.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    APPLICATION PROCEDURE

    Biosystems A25
    HEMOGLOBIN A1c Direct
    Immunoturbidimetric latex test
    2 Reagents

    Y04602 4 x 22.5 ml Reagent 1


    1 x 28.5 ml Reagent 2A
    1 x 1.5 ml Reagent 2B
    Y04606 4 x 7.5 ml Reagent 1
    1 x 9.5 ml Reagent 2A
    1 x 0.5 ml Reagent 2B
    Y04605 5 x 100 ml Hemolysis Reagent
    Y04603 4 x 0.5 ml HbA1c Direct Calibrator Set
    Y04604 4 x 0.5 ml HbA1c Direct Control Set
    1. Reagent preparation:
    Reagent 1(R) is ready to use.
    Reagent 2 (SR1) is prepared by pouring the entire contents of the R2B vial into the R2A vial. Mix gently.
    Lyse Reagent is ready to use
    2. Sample preparation
    To determine HbA1c, a hemolysate must be prepared for each sample:
    1. Dispense 1 ml Hemolysis Reagent into a tube.
    2. Add 20 µl of well mixed whole blood and mix.
    3. Allow to stand for 5 minutes or until complete lysis is evident.
    Follow the same procedure with calibrators and controls.
    3. Instrument settings:
    Without onboard Lyse Application

    GENERAL Test Name HbA1c


    Analysis Mode Endpoint bir
    Sample Type Haem
    Units %
    Reaction Type Increasing
    Decimals 1
    Replicates 1
    Name of assoc. constituent -
    PROCEDURE Type of reading monoch.
    Volumes Sample 5
    Reagent 1 240
    Reagent 2 120
    Washing 1.2
    Predilution factor -
    Filters Main 670
    Reference -
    Times Reading 1 600 s
    Reading 2 -
    Reagent 2 300 s
    Postdilution factor 3
    CALIBRATION Type of calibration Specific
    Calibrator replicates 3
    Blank replicates 3
    Calibration curve -
    OPTIONS Blank absorbance limit 1.000
    Kinetic blank limit -
    Linearitylimit 16.0

    Note: These suggested instructions and instrument parameters are to be used in conjunction with the reagent package insert and the
    instrument operation manual. Refer to these documents for complete instructions before performing tests.

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