Untitled

© 2010 Sakura Finetek USA, Inc.
All Rights Reserved

Printed in U.S.A.
Manufactured for:
Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Japan Co., Ltd., Tokyo, 135-0007, Japan
Sakura Finetek Europe B.V., 2804 AV Alphen aan den Rijn, NL
Made in U.S.A.
1320109-01 Rev C
TABLE OF CONTENTS
Section Page
1. INTRODUCTION 1.1
Safety Instructions ........................................................................................................................................ 1.1
Safety Precautions ....................................................................................................................................... 1.1
General Description ...................................................................................................................................... 1.2
Physical Characteristics................................................................................................................................ 1.3
User Interface ............................................................................................................................................... 1.5
Safety Standards .......................................................................................................................................... 1.7
2. INSTALLATION 2.1
General Information ...................................................................................................................................... 2.1
Environmental Factors .................................................................................................................................. 2.1
Unpacking..................................................................................................................................................... 2.1
Positioning the Instrument ............................................................................................................................ 2.3
3. CUSTOMIZATION OF SETTINGS 3.1

Accessing the Menu ..................................................................................................................................... 3.1
Configuring System Utilities .......................................................................................................................... 3.2
Customizing System Operating Parameters ............................................................................................... 3.11
4. OPERATING INSTRUCTIONS 4.1

Initial Setup ................................................................................................................................................... 4.1
Understanding the Control Panel Display ..................................................................................................... 4.1
Preparation ................................................................................................................................................... 4.1
Accessing the Main Menu............................................................................................................................. 4.2
Stain Process Menu ..................................................................................................................................... 4.2
Starting a Stain Process ............................................................................................................................... 4.6
Monitoring a Staining Process ...................................................................................................................... 4.7
5. ACCESSORIES 5.1
Standard Accessories ................................................................................................................................... 5.1
Optional Accessories .................................................................................................................................... 5.3
6. CARE OF THE INSTRUMENT 6.1

General Maintenance ................................................................................................................................... 6.1
7. TROUBLESHOOTING 7.1
8. SERVICE AND REPLACEMENT PARTS 8.1

Service Information....................................................................................................................................... 8.1
Replacement Accessory Items ..................................................................................................................... 8.1
Optional Accessory Items ............................................................................................................................. 8.1
Revised 3/30/2010 i
TABLE OF CONTENTS
ii Revised 3/30/2010
Section 1
INTRODUCTION
Safety Instructions Safety Precautions
The Tissue-Tek® Prisma® Automated Slide Stainer CAUTIONS and NOTES are provided throughout this
operating manual includes important instructions and manual to indicate levels of potential hazards or helpful
information related to the operating safety and information as defined below.
maintenance of the instrument. It is important to read the
CAUTION: Indicates a potential hazard in which failure
operating manual carefully prior to the operation of the
to follow instructions may result in damage to the
instrument for the first time and to keep the manual with
Prisma and/or other property.
the instrument at all times.
This instrument has been built and tested with the NOTE: Indicates a reminder or other helpful information.
following safety regulations on electrical measuring
control, regulating and laboratory devices: Safety Features
nd
IEC 61010-1 2 Ed.
nd
The Tissue-Tek Prisma Slide Stainer is equipped with
CAN/CSA C22.2 No. 61010-1 2 Ed. several safety features, which help to keep the operator
nd
UL 61010-1 2 Ed. and the instrument safe from harm.
In order to ensure safe operation, the operator must • The robotic arm will stop if the cover is opened.
always comply with the instructions and warnings • The robotic arm does not make access to the stations
contained in the operating manual. in the front row when the door is opened.
• Heater circuit is blocked off when the drying unit
CAUTION: The use of iodine on this instrument may
reaches an abnormal temperature (85ºC).
cause oxidation on some parts. It is recommended
• Heater circuit is blocked when the heating stations
that if iodine must be used in some staining methods,
reach an abnormal temperature (90ºC).
that it be removed immediately upon completion of
• Heater circuit is blocked off when the fan of the drying
the stain. Failure to remove the iodine may cause
unit abnormally stops.
oxidation.
• Heater circuit of the drying unit is blocked off when
CAUTION: Power supply plug is used as mains power the fan of the fume control system abnormally stops.
disconnecting device. Locate unit where power • Electrical circuit is blocked off when excessive current
supply is easily accessible. flows into the instrument.
NOTE: The protective devices installed on both the
instrument and accessories may neither be removed nor
modified. Only authorized and qualified persons may
access and repair the internal components of the
instrument.
The safety devices installed in this instrument by the
manufacturer only establish the basis for accident
prevention. Primary responsibility for accident-free
operation lies, above all, with the institution that owns the
instrument and, in addition, the designated personnel that
operate, service, or repair the instrument.
Revised 3/30/2010 1.1

INTRODUCTION
General Description Solution Reservoir - Includes start/end stations,
drying stations, wash stations, heating stations, solution
Tissue-Tek® Prisma® Automated Slide Stainer (Figure stations, and PE (programmed end) station.
1-A) is an automated, random-access stainer which Robotic Arm - A 3-axis transport mechanism for the
carries out multiple staining protocols simultaneously. The slide baskets.
Prisma features an open single-level design that efficiently
moves slides to various solutions for optimal performance. Fume Control System - Uses activated carbon filters,
for effectively removing harmful vapors from the inside of
The Prisma has a 10.4 inch color touch screen display the instrument.
for programming ease and stain monitoring. It is
programmable for up to 50 different staining methods, Link System - (optional) - Used to link the instrument
accommodating routine H&E, PAP, Non-GYN, and a with the Tissue-Tek Film Coverslipper.
variety of special stains or any other user defined protocol. To begin a staining process, the operator places up to
The instrument can be programmed to be started in either three baskets into a designated start station located in the
a batch or continuous mode. The batch mode allows front right of the instrument. The robotic arm picks up the
another process to start only when the previous run is basket set and sequentially moves the baskets to the
completed. In continuous mode, the user may add basket programmed stations. In the continuous mode, as new
sets in a random-access mode, maximizing efficiency and basket sets are added, the robotic arm will pick up these
throughput. To further maximize throughput, the Tissue- baskets and move them to their programmed stations
Tek Prisma can be linked to the Tissue-Tek® Film® accordingly. The basket sets will finish and be placed in
Coverslipper. This allows for a complete walk away the end stations for removal. If the optional link station and
system. the Tissue-Tek Film Coverslipper are attached to the
The Prisma consists of the following major Prisma, the stained slides will be moved over to the
components: coverslipper to be coverslipped. The closed system
design, along with the ventilation system, ensures an
Control and Monitoring System - Consists of a 10.4 essentially fume-free environment.
inch color touch screen display where all operations are
controlled.
Figure 1-A
1.2 Revised 3/30/2010

Section 1
Physical Characteristics The special stain reservoirs can be combined with the
standard or expanded configurations. The special stain
configuration (Figure 1-D) includes eight solution
Solution Reservoir Area reservoirs in a special removable reservoir tray and two
heated solution reservoirs (only available on product #
The solution reservoirs are arranged in a single-level 6131). The special stain reservoirs accommodate a
configuration and are positioned in reservoir trays. These solution volume of 160 to 180 ml.
trays can hold multiple solution reservoirs to allow for easy
placement into and removal from the instrument. The trays
are designed to ensure proper orientation when reservoirs
are placed into and, when the tray is set into the
instrument.
There are three different solution reservoirs: standard,
expanded, and special stain.
The standard configuration (Figure 1-B) contains a total
of 30 stations with six stations in the front row and eight Figure 1-D
stations each in three other rows. The standard reservoir
Also included in the solution reservoirs are the
accommodates 680-820 ml of solution.
start/end stations, wash stations, and dryer stations.
Start/End Stations - the start/end stations are located
in the front row of the reagent reservoirs. These reservoirs
have a handle for easy removal and can be programmed
as 1 to 3 start stations and up to 5 end stations. The
reservoir volume is 830 ml.
Wash Stations - the wash stations are located in the
right rear of the instrument. A maximum of four and a
Figure 1-B minimum of zero can be used at one time, depending on
The expanded configuration (Figure 1-C) contains a the setup. The reservoir volume is 850 ml.
total of 54 stations and is arranged with 6 stations in the Drying Stations - There are a total of two drying
front row and eight stations in the other six rows. The stations programmable from 30º C (86 º F) to 65º C
expanded reservoir can accommodate 255 to 285 ml of (149ºF)
solution.
Figure 1-C
Revised 3/30/2010 1.3

INTRODUCTION
Robotic Arm Water Supply
The robotic arm (Figure 1-E) can accommodate up to
One water flow line is provided to supply water to the
three baskets of twenty slides each. Each basket has a
water supply manifold for all of the wash stations. The
basket adapter that attaches to the basket holder on the
water flow rate is adjustable through an adjustment valve
robotic arm. The basket holder can be modified to
located inside the instrument (Figure 1-G) and is used to
accommodate one to three baskets using either the 20-
increase or decrease the pressure of the water flowing to
slide basket or the 10-slide basket. The function of the
the instrument.
robotic arm is to move slide baskets to specific stations on
the user-defined programs. The robotic arm moves in an
x, y, and z-axis and efficiently carries baskets to the
programmed stations.
Figure 1-G
Figure 1-E
Fume Control
The activated carbon filters (Figure 1-F) are part of an
effective fume control system. As air circulates throughout
the inside of the instrument, fumes generated by various
solutions are captured and extracted by the carbon filter.
The fume control housing can be accessed at the back,
inside of the instrument. It is recommended that the
carbon filters be replaced after two weeks with routine
operation of the stainer.
Figure 1-F
1.4 Revised 3/30/2010

Section 1
User Interface Compact Flash

The compact flash (Figure 1-J) is located on the left
side of the display screen. It is used to store programs,
Power Switch solution configurations, solution names, etc.
The power switch (Figure 1-H) is located on the lower
right side of the instrument. The power switch also acts as
an emergency stop switch.
Figure 1-J
Figure 1-H External Interface

The external interface (Figure 1-K) is located on the
right side of the instrument and includes the following:
Display Screen
Power outage detection
The display screen (Figure 1-I) is a 10.4 inch VGA
External Alarm output
(Video Graphics Array) TFT color touch screen. It is
located on the right side of the instrument on an Printer output (RS232)
adjustable arm. All operating functions are controlled Ethernet (10-BaseT) port
through the display screen (e.g. displays predicted end
time, run numbers, and staining methods, etc.)
Figure 1-I
Figure 1-K
Revised 3/30/2010 1.5

INTRODUCTION
Power Cord
The power cord (Figure 1-L) is located on the
instrument right side panel and is used to connect the
instrument to a power source. The power cord must be
plugged into an appropriately grounded AC electrical
outlet.
Figure 1-L
Drain Hose Port

The drain hose port (Figure 1-M) is located on the rear
instrument panel and allows for sufficient draining of the
Prisma.
Figure 1-M
1.6 Revised 3/30/2010

Section 1
Specifications Dimensions
Models Centimeters: 125 (W) x 71.3 (D) x 63 (H) cm
Inches: 49.3 (W) x 28 (D) x 24.8 (H) in.
Model Name/Description
Weight: 150 kg (330 lbs.)
Tissue-Tek® Prisma® Automated Slide Stainer (115 VAC)
6130
(USA)
Operating Conditions
Tissue-Tek® Prisma® Automated Slide Stainer with Special
6131
Stains (115 VAC) (USA) Operating Temperature: 10º C to 40º C (50º F to 104º F)
Tissue-Tek® Prisma® Automated Slide Stainer (230 VAC) Relative Humidity: 30-85% (non-condensing)
6132
(Europe)
Atmospheric Pressure: 80 to 106 kPa
Tissue-Tek® Prisma® Automated Slide Stainer with Special
6133
Stains (230 VAC) (Europe)
Storage Conditions
Tissue-Tek® Prisma® Automated Slide Stainer (100 VAC)
6120 Storage Temperature: -20º C to 65º C (-4ºF to 149º F)
(Asia)
Tissue-Tek® Prisma®
Automated Slide Stainer with Special Relative Humidity: 20-90% (non-condensing)
6121
Stains (100 VAC) (Asia)
Atmospheric Pressure: 50 to 106 kPa
Power Ratings and Requirements

Model Voltage Frequency Amps
6130 115 VAC ± 10% @ <15A 60 Hz 9.0A
6131 115 VAC ± 10% @ <15A 60 Hz 10.0A
6132 230 VAC ± 10% @ < 7A 50/60 Hz 4.5A
6133 230 VAC ± 10% @ < 7A 50/60 Hz 5.0A
6120 100 VAC ±10% @ <15A 50/60 Hz 8.0A
6121 100 VAC ±10% @ <15A 50/60 Hz 9.0A
Safety Standards
Complies with IEC 61010-1 2nd ed., CAN/CSA C22.2 No.
61010-1, UL 61010-1
CE registered in compliance with the requirements of
EMC Directive, LV Directive in Europe, In Vitro Diagnostic
Device Directive.
Revised 3/30/2010 1.7

INTRODUCTION
Water Supply Performance Data:
Water Pressure: Batch Operation:
Maximum Static Pressure: 0.83 Mpa
Up to 60 glass slides in three baskets. (3 baskets = 1
Dynamical Pressure: 0.098 to 0.441 Mpa basket set)
Water Temperature: Lower than 30º C (86 º F),
no freezing Continuous Operation:
Diameter of Plumbing: More than 1.27cm (1/2”) Basket sets can be continually loaded on demand as
the start station(s) become available for loading. Up to 60
Drain glass slides in three baskets for each loading station.
Drain: Gravity Programs/Methods

Drain Capability: More than 15 liters per minute, Up to 50 programs can be stored in memory.
anti-backflow pressure
Up to 50 steps can be programmed for each method.
Diameter of Plumbing: More than 40A (1 1/2”)
Reservoir Capacity/Fill Volumes

Type:
Standard Reservoirs: (total) - 30
Expanded Reservoirs: (total) - 54
Special Stain Reservoirs* - 8
Wash Reservoirs - 4
Dryer Stations - 2
Heater Stations *- 2
Capacity:
Standard Reservoirs: 680-820 ml
Expanded Reservoirs: 255-285 ml
Wash Reservoirs: 850 ml (volume up to the
overflow level)
Start/End Reservoirs: 690-830 ml
Special Stain Reservoir: 160-180 ml
Heater Solution reservoir*: 240-250 ml
*-Only for model # 6131
1.8 Revised 3/30/2010

Section 1
Solution Names
Up to 100 solution names (20 characters per name)
can be stored in memory.
Staining Method Names

Up to 50 staining method names can be stored in
memory.
Solution Configurations
Up to 50 solution configurations can be stored in
memory.
Revised 3/30/2010 1.9

INTRODUCTION
1.10 Revised 3/30/2010

Section 2
INSTALLATION
General Information
This section provides detailed installation and setup
instructions for the Tissue-Tek® Prisma® Automated Slide
Stainer. The installation steps must be followed correctly
to ensure proper operation and service. Read this
operating manual carefully before attempting to operate
the instrument. Follow all instructions carefully.
The Tissue-Tek Prisma is a precision instrument and
must be handled accordingly. Rough handling or dropping
the instrument will disturb or damage internal components.
Always handle the instrument with care.
Select a place where sufficient clearance can be
provided around the instrument. Ample working space is
necessary in front and on top of the instrument. Figure 2-A
2. Remove the lid of the box (Figure 2-B).
Environmental Factors
As with all sensitive electronic instruments, prolonged
exposure to excessive humidity and temperature should
be avoided. Temperature and humidity should be held
relatively constant. The ambient temperature for operating
the instrument is 10º C to 40º C (50º F to 104º F). The
ambient operating humidity range is 30-85% relative
humidity.
Unpacking
Removing the Outer Shipper

Figure 2-B
Inspect the carton and make sure there are no visible
signs of damage. If visible signs of damage are evident, 3. Remove the Styrofoam pieces surrounding the
immediately file a complaint with the carrier and notify instrument. The outer cardboard box may now be
Sakura Finetek USA Technical Support Department. In lifted up and away from the instrument.
countries other than the USA, contact the nearest 4. The instrument will be positioned on the pallet and
authorized Sakura distributor or representative. covered by a protective wrapping and Styrofoam
1. If no visible signs of damage are evident, remove the packing material. If the Styrofoam has not already
protective tie wraps (Figure 2-A) securing the outer been removed, remove it from the corners of the unit.
shipper box to the pallet. Remove the protective wrapping exposing the
instrument.
5. Four L-shaped brackets anchor the pallet to the
instrument. Remove all four bolts that fasten the
bracket to the instrument.
Revised 3/30/2010 2.1

INSTALLATION
6. Remove the nut located on the underneath portion of 11. Carefully lift the instrument from the pallet and place it
the pallet using a wrench to hold the bolt (Figure 2-C). on a sturdy, level surface capable of supporting
150 Kg (330 lbs.)
CAUTION: The instrument is very heavy and large,
therefore, it is strongly recommended that it always
be lifted and transported by at least four people, one
positioned on each corner of the instrument.
CAUTION: Always transport the instrument in an
upright position.
12. Remove any adhesive tape from the outside of the
instrument.
13. Open the cover and remove all tape and packing
material (Figure 2-E).
Figure 2-C
7. Remove all tape and packing foam from the outside
of the instrument.
8. Open the main cover on the instrument and locate the
accessory box positioned inside.
9. Cut the tie-wraps holding the accessory box. Remove
the tie-wraps carefully from around the support post.
10. Remove the accessory box from inside the instrument
(Figure 2-D).
Figure 2-E
14. Remove the packing and adhesive tape from the
display.
15. Remove the power cord from the plastic bag.
NOTE: Be certain that all tape and foam packing material
is removed from the instrument prior to operation.
Figure 2-D
2.2 Revised 3/30/2010

Section 2
Unpacking Check List Optional Accessories (Link System)-For use with the
When opening the accessory box, confirm that all Tissue-Tek Film Automated Coverslipper. Tissue-Tek Link
accessories have been included with the instrument. System (1)-6134 consists of:
r Operating Manual (1) r Installation Bar (1)
r Warranty Card (1)-located inside the operating r Bolts, SW, W (5 Each)
manual (USA only) r Communication Data Cable (1)
r Slide Baskets (10) r Compact Flash (program for connection) (1)
r Slide Basket Adapter (10) If any of these items are missing, contact the Technical
r 820 ml Solution Reservoir (22) Support Department at Sakura Finetek USA. In countries
r 830 ml Load/Unload Reservoir (6) outside the USA, contact the nearest Sakura distributor or
representative.
r 850 ml Wash Reservoir (4)
r 3-position Reservoir Lid (3)
r 4-position Reservoir Lid (3) Positioning the Instrument
r 3-position Reservoir Tray (3) Locate the instrument in a well-ventilated area,
r 4-position Reservoir Tray (3) avoiding exposure to corrosive vapors and extreme
r Activated Carbon Filters (2/cs) variations in temperature or humidity. The area should be
clean, dust free, and have a firm, level surface capable of
r Compact Flash Card (1) holding at least 150 kg (330 lbs.) of weight. Be sure it is
r Control Panel Protection Sheet (installed) (1) near a power source that meets the electrical
r Drain Hose (1) requirements specified on the rating label located on the
r Drain Hose Clamp (1) right side of the instrument. The power receptacle must be
grounded and should be a dedicated line. Avoid proximity
r Drain Elbow (1)
to direct sunlight, open windows, ovens, hot plates, open
r Water Supply Hose Set (1) burners, or radiators.
r Station Labels (1)
r Wash Station Plug (4) (4 installed) Leveling the Instrument
r Single Reservoir Lid (6)
Once the instrument is placed on a sturdy, level
surface, it is important that it is leveled.
Optional Accessories –Special Stain Set (If Product #
Using a wrench, adjust the feet by turning the nut on
6131 was ordered)
each foot. Turning the nut clockwise will raise the
r 10-slide Basket (10)-6137 instrument, counter-clockwise will lower the instrument.
r 10-slide Basket Adapter (10)-6138 Place a level on the inside, middle of the instrument
r 10-slide Basket Load/Unload Adapter (3)-6139 and adjust the feet until the instrument is level.
r 160 ml Special Stain Solution Reservoir (8)-6140 NOTE: Being certain that the instrument is level will
r Heated Solution Reservoir (2)-6141 ensure proper water drainage and overall instrument
r Special Stain Reservoir Lid (8)-6144 operation.
r Special Stain Reservoir Tray (1)-6156
Revised 3/30/2010 2.3

INSTALLATION
Positioning the Accessories 7. Push firmly against the filter cover to close and latch
the hook.
Installing the Activated Carbon Filters
NOTE: It is recommended that the carbon filters be
CAUTION: Make sure that the instrument is switched replaced periodically. See Section 6, Care of the
off and the power cord is unplugged before Instrument for maintenance schedule.
beginning.
1. Open the main cover of the instrument. Installing the Water Drain Hose
2. Locate the filter cover located on the rear panel inside CAUTION: The drain hose should be installed before
of the instrument. the water is supplied to the instrument.
3. Open the filter cover by pressing down on the button 1. Locate the drain hose port located on the lower, rear
located at the top center of the filter cover (Figure of the instrument (Figure 2-H).
2-F).
Figure 2-H
2. Insert the elbow fitting.
3. Place the drain hose assembly onto the elbow fitting
Figure 2-F and tighten the connecting hose clamp to ensure that
4. Lower the filter cover. it is properly seated.
5. Remove the plastic wrapping from both carbon filters. 4. Attach the drain hose to the drain hose assembly. Be
sure that the clamped end of the hose attaches to the
6. Place the carbon filters side by side in a horizontal elbow. Tighten the clamp by using a Phillips
position in the appropriate location (Figure 2-G).
screwdriver. The other end of the drain hose should
be routed to a floor or sink drain that is capable of
draining at a rate of 15 liters (4 gallons) per minute.
CAUTION: Make sure that the entire length of the
drain hose is lower than the drain port. It should be
straight and not twisted or looped. Do not extend the
drain hose by coupling it with another hose. The end
of the drain hose should be at least 4 inches (100 mm)
or higher from the water surface in the floor or sink
drain.
Figure 2-G
2.4 Revised 3/30/2010

Section 2
Installing the Water Supply Hose 3. Grasp the reservoir by the handle and insert it into
one of six positions located in the front row of the
1. Connect the pre-assembled water supply hose to the
solution reservoirs (Figure 2-J).
water supply port located at the bottom, right of the
instrument (Figure 2-I).
Figure 2-J
4. Close the front door.
Dryer Stations
Figure 2-I
CAUTION: Make sure that the instrument is switched
2. Connect the other end of the hose to a faucet or off and the power cord is unplugged before
water source. beginning.
NOTE: Use plumbers tape to be certain that the water 1. Remove the 2-piece drying station assembly from the
supply hose is securely connected to the instrument. accessory box.
CAUTION: When installing the water supply hose, 2. Place the large piece inside the drying station (Figure
make sure it is securely connected to the instrument 2-K) using the diagram provided near the drying
and to the faucet. Failure to do so may allow the water station as a guide.
pressure to loosen the connection and cause an
overflow.
CAUTION: It is recommended that the water supply to
the instrument be turned off when the laboratory is
left unattended, i.e. overnight or weekends.
Installing Solution Reservoirs

Start/End Stations
1. Open the door located in the front of the instrument
by pressing on the magnetic latch.
2. Remove the solution reservoirs with handles from the Figure 2-K
accessory box.
Revised 3/30/2010 2.5

INSTALLATION
3. Place the slotted piece over the top of the larger piece To transform a wash station to a solution station:
(Figure 2-L).
1. Remove the wash reservoir. Turn the wash cap
counter-clockwise and remove the wash nozzle and
gasket from the instrument (Figure 2-N).
Figure 2-L
4. Repeat the procedure in the second drying station.
Figure 2-N
Wash Stations
2. Replace the wash nozzle with a wash station plug
The wash stations are positioned in the right rear of the and secure it in place using the wash cap.
solution reservoirs. A maximum of four and a minimum of
3. Place a solution reservoir at that position.
zero wash stations may be used at one time. The factory
default setting is set at four wash stations. The solution To transform a solution station to a wash station:
configuration may need to be set up before a decision is
made to make changes to remove the factory set wash 1. Turn the wash cap counter-clockwise and remove the
stations. wash cap, and gasket.
1. Position the wash stations with the opening in the rear 2. Replace the wash station nozzle and secure all items
of the reservoir toward the back (Figure 2-M). This will in place.
allow for the water to overflow from the reservoir into 3. Place a wash station at this position.
the drain. NOTE: Verify that the station configuration reflects the
proper wash positions.
Figure 2-M
2.6 Revised 3/30/2010

Section 2
Heating Stations NOTE: The solution reservoir trays are configured to be

positioned only one way in the instrument. This ensures
The heating stations are positioned to the right of the proper orientation.
solution reservoirs at the front of the instrument.
2. Position the tray on a level surface and place the
NOTE: Heating stations are only available on product solution reservoir in the tray.
#6131.
NOTE: The solution reservoirs are designed to fit into the
1. Position the heating stations as shown in Figure 2-O. reservoir trays in one direction to ensure proper
orientation.
NOTE: There are two different solution reservoir trays: a
three-position tray and a four-position tray. Appropriately
place solution reservoirs.
3. The solution reservoirs can be filled with solutions
now or placed back into the instrument (Figure 2-Q)
for filling at a later time.
Figure 2-O
Solution Reservoirs
The Prisma comes with six solution reservoir trays. The
trays can be easily removed to fill and position the solution
reservoirs.
1. Grasp a reservoir tray by the handles located on each Figure 2-Q
side and remove the tray by lifting it straight out
(Figure 2-P).
Figure 2-P
Revised 3/30/2010 2.7

INSTALLATION
Installing Compact Flash Installing 20-Slide Load/Unload Adapter
The compact flash card is used to store programs, If using the expanded solution configuration, an
solution configurations, solution names, etc. It is to be adapter must be added to the start stations to alert the
positioned on the left upper corner of the color touch instrument that only 20 slides will be run.
screen. 1. Remove the three-arm basket adapter to
1. Turn the instrument off using the power switch. accommodate only one basket (Figure 2-S).
2. Position the compact flash card into the designated NOTE: If the three-arm basket is not removed, the basket
area (Figure 2-R). holder may become misaligned resulting in a robotic arm
malfunction.
Figure 2-R
Figure 2-S
3. Press flash card in fully.
2. Place insert into start stations (Figure 2-T).
4. Power instrument on with the power switch.
Figure 2-T
NOTE: The instrument will automatically detect that only
one 20-slide holder is being placed into the start station.
2.8 Revised 3/30/2010

Section 2
Electric and Link Connections

Connecting the Power Cord
1. Make sure the power switch is in the “O” position.
2. Locate the power cord positioned on the rear of the
instrument.
3. Plug the other end of the power cord into an
appropriate AC electrical outlet. The outlet should be
a dedicated line and it must be grounded.
CAUTION: Do not connect or disconnect power
supply with wet hands. Doing so may cause electrical
shock.
Figure 2-U
CAUTION: Connection/Disconnection of the power
cord by holding any other part but the plug body may 3. Fill the reservoirs with the desired solutions.
cause fire or electrical shock. 4. Remove the three-arm basket adapter to
4. Switch the power switch to the on position labeled “I”. accommodate only one basket (Figure 2-V).
Setting up the Link Connection (Optional) NOTE: If three-arm basked adapter is not removed, the
basket holder may become misaligned resulting in a
A data cable is part of the optional link connection that robotic arm malfunction.
connects the Prisma to the Tissue-Tek Film Coverslipper.
1. Connect one end of the data cable to the link inlet
located on the lower left of the instrument, and the
other end to the rear of the Film Coverslipper.
NOTE: Optional link station must be installed by an
authorized Sakura Representative.
Installation of Special Stain Accessories

for Product #6131 (Prisma with Special
Stains)
If product #6131 is being used, a reservoir tray
including eight special stain reservoirs comes as a
standard accessory.
Figure 2-V
1. Remove the standard reagent reservoir tray from the
instrument (the row directly underneath the wash
stations).
2. Replace the reservoir tray with the special stain
reservoir tray (Figure 2-U).
Revised 3/30/2010 2.9

INSTALLATION
5. Place insert into start stations (Figure 2-W) that
indicate the use of the special stain 10-slide basket.
Figure 2-W
NOTE: The insert must be placed into the start stations if
a 10-slide basket is used. The instrument will
automatically sense that a 10-slide basket is being placed
into the start station.
NOTE: It is important to remove the special stain
reservoir tray if running other configurations that do not
include those reservoirs. If left in place, the other
configurations will not show up.
2.10 Revised 3/30/2010

Section 3
CUSTOMIZATION OF SETTINGS
General Information
The Tissue-Tek® Prisma® Automated Slide Stainer
allows for the customization of a number of system
options and the configuration of certain operating
parameters to suit the specific requirements of a given
installation. The Prisma Menu provides the means of
viewing and modifying current system settings.
This section outlines the procedures to perform initial
setup of Tissue-Tek Prisma system settings. Factory
default settings for each parameter are listed where
applicable.
Perform the following setup procedures prior to initial
use of the system. In addition, perform these procedures
as needed to reset system settings, add or remove system
users, etc. 3. Touch the Menu button to display the main menu.
Accessing the Menu

Note: You must login as an Administrator in order to
modify system settings and operating parameters.
To access the Menu:
1. From the main system screen (Stain Process Monitor
screen), touch the Log On button. The Enter
Password dialog box displays.
2. Use the touch keypad to enter your password and
then touch the Enter button.
• The default password for the system is set to
“100000”.
Note: For security reasons, your password will display on The Tissue-Tek Prisma Menu consists of three tabbed
the screen, as entered, as a series of asterisks. pages that provide access to the following submenus
• If your login is unsuccessful: Re-enter your Utility Menu, Edit Menu, and Stain Process Menu.
password and then press the Enter button to System configuration and setup are performed from the
resubmit your login request. Utility and Edit menus. The Stain Process Menu is used
for system operation and is described in Section 4,
• If your login is successful: The Stain Process
Operating Instructions.
Monitor screen displays.
Revised 3/30/2010 3.1

Configuring System Utilities System Setup
The Utilities Menu is used to configure and setup The System Setup option from the Utility Menu lets you
Tissue-Tek Prisma system options. make changes to “user-selectable” and “user-specified”
system settings and parameters.
To access the Utility Menu: 1. From the main menu, touch the Utility Menu tab, and
1. Log on. then touch the System Setup button.
2. From the main Menu, touch the Menu button and • The System Setup screen displays.
then touch the Utility Menu tab.
The System Setup screen allows users to adjust

The following options are available from the Utilities system date and time, set user passwords and access
Menu. authorizations, manage fume filters and change the
institution name that appears on each screen.
Utility Menu 2. Make changes to the following system
System Setup – allows for the configuration of settings/parameters, as desired:
user-selectable options and operating • Date Format – sets the format for the date
parameters (see System Setup on page 3-2) display shown at the top of the instrument display
Language Selection – allows the user to screens. Touch the option button until the desired
select the system display language. Seven format displays. Available choices are
language options are available. Year/Month/Day, Month/Day/Year and
Export Data – provides options for exporting Day/Month/Year.
Tissue-Tek Prisma data to a compact flash The factory default setting is Year/Month/Day.
card or to a printer. • Time Format – sets the format for the time
Water Flow – allows the user to set the run-up display shown at the top of all instrument display
time in the water stations. screens. Touch the option button until the desired
format displays. Available choices are 12 hour
Arm Movement – provides the user with a format and 24 hour format.
means to verify function of the robotic arm The factory default setting is 12 hour format.
• Key Sound – sets the sound off or on. If the
Service Operations – this option is only sound is on, an audible tone is heard each time a
available to certified Sakura service personnel. key or button is touched on the screen.
The factory default setting is ON.
3.2 Revised 3/30/2010

Section 3
• Alarm Tone and Volume - sets the tone and

volume for system alarms. Available choices are
Soft and High, Loud and Low, Loud and High and
Soft and Low.
The factory default setting is Loud and High.
• Export Data – sets the destination path for data
export. Available choices are Off, Memory Card
or Printer.
The factory default setting is Memory Card.
• Coverslipper Link – establishes a link between
the Tissue-Tek Prisma and the Tissue-Tek Film
Coverslipper for those institutions using both
instruments. Available choices are Not in Use or
In Use. This option is only available if the
3. Use the up and down arrows to change the current
Coverslipper link is installed.
month.
The factory default setting is Not in Use.
4. Use the touch screen to select the current date.
Changing Date and Time 5. Under Time Setup, touch the Modify button beneath
the current hour to modify hour.
1. From the System Setup screen, touch the Date and
6. Touch the Modify button beneath the current minutes
Time button.
to modify minutes.
• The Date and Time Screen displays.
7. Touch the am/pm button to change from am to pm.
8. Touch Save and then Exit to save your settings or
• Touch Exit to leave the screen without saving
your changes.
Setting User Names and Passwords

1. From the System Setup screen touch the Password
Input button.
• The Password Setup screen displays. This
screen allows you to create user ids (user id is
the name that appears on all screens when a
given user is logged in) and passwords, grant or
deny screen access and modify existing users.
The system allows for four Administrators and
twenty Users.
2. Under Date Setup, touch the Modify button to
change the Year setting. An input screen displays.
Touch the Clear button to clear the year being
displayed and then use the keypad to touch the
appropriate numbers to represent the current year. If
you make a mistake, use the clear button to clear the
selection and start over. Touch Enter to save your
changes or Cancel to exit the screen without saving.
Revised 3/30/2010 3.3

To add or change user passwords:
1. From the Administrator/User list on the left of the
Password Setup screen, select the user or
administrator for whom you want to add or change
passwords and then touch the Password Setup
button.
• The Password Setup screen displays.
To change User ID
screen, select the user or administrator for whom you
want to create a user id then touch the User’s ID
button.
• The Create User’s ID screen displays.
2. Use the keypad to enter a numeric password between

1 and 6 digits and then touch the Enter button. The
Password Confirmation screen displays.
3. Use the keypad to re-enter the new password and
touch Enter.
• Touch Save to save the new password and
return to the Password Setup screen or
• Touch Exit to return without saving.
To grant screen access:

Users can be allowed or denied access to certain
system screens and functions.
2. Touch the Clear button to erase the existing name Password Setup screen, select the user or
and then use the keypad to enter the new ID. administrator for whom you want to change screen
• Touch the Save button to save your changes and access permissions and then touch the Screen
return to the Password Setup screen or Access button.
• Touch Cancel to return without saving your • The Password Setup>Screen Access screen
changes. displays. This screen displays three tabbed
pages; Stain Process, Utility and Edit. Each
tabbed page enables the administrator to grant or
deny a user access to specific functions.
3.4 Revised 3/30/2010

Section 3
Stain Process – sets permissions for Staining Edit – sets permissions for Edit Program,
Mode Selection, Abort Stain Process, Reset Program Check, Edit Solution Configurations and
Solution Usage Information, and Solutions Solutions Name and Delete Data. The default
Configuration Selection. The default setting for setting for each function is Allowed. Touch each
each function is Allowed. Touch each function as function as necessary to change permissions
necessary to change permissions from Allowed from allowed to Not Allowed. Touch Save to save
to Not Allowed. Touch Save to save settings settings and return to the Password Setup screen
and return to the Password Setup screen or or touch exit to return without saving changes.
touch exit to return without saving changes.
Utility – sets permissions for System Setup,
Export Data, Language Selection, Water Flow.
The default setting for each function is Allowed.
Touch each function as necessary to change
permissions from allowed to Not Allowed. Touch
Save to save settings and return to the Password
Setup screen or touch exit to return without
saving changes.
Revised 3/30/2010 3.5

Changing Fume Filter Management
Settings
1. From the System Setup screen touch the Fume Filter
Management button.
• The Fume Filter Management screen displays.
2. Touch Clear to clear the current setting and then use

the touch keypad to enter the new setting.
• Touch Enter to save the new setting and return
to the Fume Filter Management Setup screen or
• Touch Cancel to return without saving new
settings.
Fume Filter Management can be enabled or disabled. To reset actual time
To use fume filter management: 1. To reset the actual usage time for the current filters,
touch the Reset Actual Time button.
• Toggle the button at the top-left of the screen
until it reads In Use. • A confirmation dialog box appears.
• To disable fume filter management, toggle the
button until it reads Not in Use.
To adjust fume filter usage time limit:
1. Touch the Set Time Limit button.
• An input dialog box appears.
The default Usage Time Limit is 240 hours.
• Touch Yes to confirm that actual time is to be

reset to 0 or
• No to abort the action.
3.6 Revised 3/30/2010

Section 3
Creating or Changing Institution Name

The name of the institution that appears on the top of
each of the instrument display screens can be modified as
follows:
1. From the System Setup screen touch the Institution
Name button.
• The Create Institution Name screen displays.
2. Use the touch screen to select the language to be

used for system display. Options are English
(default), Japanese, German, French, Italian, Spanish
and Korean.
• Touch Save to save the selection and return to
the Utility menu or
• Touch Exit to return without saving changes.
Note: If Korean is selected, a special notice appears
2. Touch Clear to clear the current display and then use regarding copyright of the font used. Touch Exit to close
the touch keypad to enter a new name up to twenty the Notice screen.
characters long.
• Touch Save to save changes and return to the
system setup screen or
• Touch Cancel to return without saving changes.
Selecting System Language

1. From the main menu, touch the Utility Menu tab, and
then touch the System Language button.
• The Language Selection screen displays.
Revised 3/30/2010 3.7

Exporting System Setup Data 2. Touch to select the program to be exported and then
Once system setup procedures have been completed, touch the Export button.
data can be exported to either a compact flash card or a • To export all programs, touch the All Data button
printer. It is recommended that data be exported into one and then touch the Export button.
of these formats so that a record of system setup settings • An export confirmation dialog box displays, touch
is maintained. This data can be used to recreate system the Exit button to return to the Export Data
settings in the event of system failure or can be used to screen.
set up similar systems.
To export a process report
To export data:
1. From the Export Data screen, touch the Process
1. From the main menu, touch the Utility Menu tab, and Report button and then touch Export.
then touch the Export Data button.
• An export confirmation dialog box displays, touch
• The Export Data screen displays. the Exit button to return to the Export Data
screen.
To export a solution name list
1. From the Export Data screen, touch the Solution
Name List button.
the Exit button to return to the Export Data
screen.
To export a solution configuration name list
Name List button.
screen.
To export Solution Configuration(s)
To export a program name list
Configuration button. 1. From the Export Data screen, touch the Program
• A Solution Configuration List displays. Name List button.
2. Touch to select the solution configuration to be • An export confirmation dialog box displays, touch
exported and then touch the Export button. the Exit button to return to the Export Data
screen.
• To export all configurations, touch the All Data
button and then touch the Export button. To export a system setup data
• An export confirmation dialog box displays, touch 1. From the Export Data screen, touch the System
the Exit button to return to the Export Data Setup button.
screen.
To export program(s) the Exit button to return to the Export Data
screen.
1. From the Export Data screen, touch the Program
button. • Solution Usage Status
• A Program List displays.
3.8 Revised 3/30/2010

Section 3
To export solution usage status

Usage Status button.
screen.
Adjusting Water Flow

The Water Flow option allows the user to set the run-up
time for water flow during staining.
Note: Ensure that all water has been completely drained
from all wash stations in use prior to beginning the water
flow test. 3. Once the water starts to flow, watch the water level
1. From the main menu, touch the Utility Menu tab, and until it reaches the opening in the back of the wash
then touch the Water Flow button. reservoir. To verify flow rate, wait until the wash
• The Water Flow screen displays. reservoirs start to overflow and then touch the Stop
button.
• A message dialog box appears.
2. Touch the Start button.

• The following screen displays:
• Touch Save to use the displayed run-up time or
• Touch Exit to return to the Water Flow screen.
4. If necessary, repeat steps 2 and 3 or touch Exit to
return to the Utility Menu.
Revised 3/30/2010 3.9

Checking Robotic Arm Movement 2. Touch Start to perform arm movement check or Exit
1. From the main menu, touch the Utility Menu tab, and to return to the Utility Menu.
then touch the Arm Movement Check button. • Once the arm movement check is in progress,
• The Arm Movement Check message box screen touch Stop to cancel the check.
displays.
3.10 Revised 3/30/2010

Section 3
Customizing System Operating Creating or Changing Solution Names

Parameters The Edit Solution Name option from the Edit Menu tab
lets the user add, edit, copy or export solution names. The
The Edit Menu provides access to several options that system stores up to 100 solution names.
allow the user to configure the system operating
definitions, settings and programs that should be To add a new solution name:
established prior to system use.
1. From the main menu, touch the Edit Menu tab, and
To access the Edit Menu: then touch Edit Solution Name.
• The Edit Solution Name screen appears.
1. Log on.
2. From the Main Menu, touch the Menu button and
then touch the Edit Menu tab.
2. Touch Add Solution Name.

• The Create Solution Name screen displays.
The following options are available from the Edit Menu.
Edit Menu
Edit Solution Name – allows the user to
modify the name of the solution.
Edit Solution Configuration – provides a

means to add or edit solution configuration
Edit Program – allows the user to add or edit

existing programs for routine operation
Program Check – enables the user to select

a solution configuration and program to
ensure that they are compatible.
Delete Data – Allows the user to delete 3. Use the touch keyboard to enter a new solution name
unwanted information (solution names, up to twenty characters long.
programs and solution configurations) from • Touch Save to save the new solution name and
the system. return to the Edit Menu or
Revised 3/30/2010 3.11

• Touch Cancel to return without saving the new 2. On the Solution List, touch to highlight the solution
name. name to be edited, and then touch the Edit button.
• The Create Abbreviated Solution Name screen • The Create Solution Name screen displays.
displays.
3. Use the touch keyboard to modify the solution name

4. Use the touch keyboard to enter an abbreviated name using up to twenty characters.
for the new solution up to eight characters long. This • Touch Save to save the solution name and return
name is used for on-screen display. to the Edit Menu or
• Touch Save to save the abbreviated solution • Touch Cancel to return without saving the new
name and return to the Edit Menu or name.
• Touch Cancel to return without saving the new • The Create Abbreviated Solution Name screen
name. displays.
To edit solution name:
1. From the main menu, touch the Edit Menu tab, and
then touch Edit Solution Name.
• The Edit Solution Name screen appears.
3.12 Revised 3/30/2010

Section 3
4. Use the touch keyboard to enter an abbreviated name 2. Touch the Add Configuration button.
for the new solution up to eight characters long. This • The Edit Solution Configuration Screen displays.
name is use for on-screen display.
• Touch Save to save the abbreviated solution
name and return to the Edit Menu or
• Touch Cancel to return without saving the new
name.
Creating or Changing Solution

Configurations
The Edit Solution Configuration option from the Edit
Menu tab provides the means to add or edit solution
configurations. The system can store up to 50 solution
configurations.
To add a new solution configuration:
1. From the Edit Menu, touch the Edit Solution 3. Set the following parameters for the solution
Configuration button. configuration:
• The Edit Solution Configuration screen displays. Solution Configuration Type Standard or Expansion
Number of Start Stations 1 to 3 Stations
Number of End Stations 1 to 3 Stations
Program End (PE) Stations In Use or Not in Use
Number of Wash Stations 0 to 4 Stations
Special Solution Stations In Use or Not in Use
Heating Stations In Use or Not in Use
Drying Stations In Use or Not in Use
Note: The programmed end stations will automatically be
positioned in the end station located on the far right.
Revised 3/30/2010 3.13

4. Touch Save. • Each square represents a station.
• The Create Solution Configuration Name screen Note: Start/End, drying and wash stations are blocked.
displays.
8. Select an available station to assign the configuration
• Create solution configuration to.
• The Edit Solution Configuration> “Selected
Station” screen Displays.
5. Use the touch keyboard to enter a solution

configuration name, up to 20 characters long and
then touch Save. 9. Under Solution Name, use the Solution List to select
• Touch Cancel to exit without saving. and highlight a solution to attribute to the selected
The Create Abbreviated Solution Configuration Name station.
Screen Displays. • If your reagent is not listed or is listed incorrectly
6. Use the touch keyboard to enter an abbreviated use the Add Solution Name or Edit Name
solution name (to display on the station button), up to buttons.
5 characters long. 10. Touch the Management Method button to select a
7. Touch Save to save the name. Management Method of either Days or Runs
• The Edit Configuration 早Solution Configuration
Name旨 screen displays
3.14 Revised 3/30/2010

Section 3
11. Under Set Limit Value, touch Modify. Note: The programmed end stations will automatically be
• A numeric keypad displays. positioned in the end station located on the far right.
5. Touch Save.
• The Create Solution Configuration Name screen
displays.
6. If necessary, use the touch keypad to modify the
solution configuration name, up to 20 characters long
and then touch Save.
• Touch Cancel to exit without saving.
• The Create Abbreviated Solution Name Screen
Displays.
7. If necessary, use the touch keyboard to enter an
abbreviated solution name (to display on the station
button), up to 8 characters long.
8. Touch Save to save the name.
• The Edit Configuration 早Solution Configuration
12. Using the touch keypad, enter a number value to set Name旨 screen displays
the limit for either runs or days • Each square represents a station.
13. Touch Save. 9. Select the station to assign the configuration to.
• A message dialog box displays.
Note: Start/End, drying and wash stations are blocked.
14. Touch Save and Exit to save the new solution
• The Edit Solution Configuration> Selected
configuration.
Station screen Displays.
To edit a solution configuration: 10. Under Solution Name, use the Solution List to select
1. From the Edit Menu, touch the Edit Solution and highlight a solution to attribute to the selected
Configuration button. station.
• The Edit Solution Configuration screen displays. 11. Select a Management Method days or runs
2. From the Solution Configuration List, select to 12. Under Set Limit Value, touch Modify.
highlight the solution configuration to be modified and • A numeric keypad displays.
then touch the Edit button. 13. Using the touch keypad, enter a number value to set
3. The Edit Solution Configuration Screen displays. the limit for either runs or days
4. Set the following parameters for the solution 14. Touch Save.
configuration: • A message box displays.
Solution Configuration Type Standard or 15. Touch Save and Exit to save the new solution
Expansion configuration.
Number of Start Stations 1 to 3 Stations
Number of End Stations 1 to 3 Stations
Program End (PE) Stations In Use or Not in Use
Number of Wash Stations 0 to 4 Stations
Special Solution Stations In Use or Not in Use
Heating Stations In Use or Not in Use
Drying Stations In Use or Not in Use
Revised 3/30/2010 3.15

To copy a solution configuration:
1. From the Edit Menu, touch the Edit Solution
Configuration button.
• The Edit Solution Configuration screen displays.
2. From the Solution Configuration List, select to
highlight the configuration to be copied, and then
touch the Copy button.
• The Copy Solution Configuration screen displays.
3. Touch Exit to return to the Edit Solution Configuration

screen.
Editing Station Properties

The Station Properties option allows the user to change
station colors as displayed on-screen and to modify
station parameters.
To access station properties options:
3. From the Copy To list, select to highlight the solution Configuration button.
configuration to copy configuration information to and 2. Select a solution configuration.
then touch the Copy button. 3. Touch the Station Properties button.
• To create a new solution configuration to copy • The Station Properties screen displays.
the configuration data to, touch the Add
Configuration button and follow the add a new
configuration procedure on page 3-13 to add
the new configuration.
To view solution configurations:
This option allows the user to view a list of current
solution configurations.
Configuration button.
2. Touch the Detailed View button.
• Solutions configurations display in list format.
3.16 Revised 3/30/2010

Section 3
To change station colors:

1. From the Station Properties screen, touch the Station
Colors button.
• The following screen displays.
From this screen you can setup Mix Parameters, setup

Enhanced Wash, change Basket lifting speed, change
water wash cycle time and adjust temperature settings.
To adjust mix parameter settings.
1. From the Station Parameters screen, select to
2. For each station, select between eight different colors highlight the station to be adjusted and then touch the
to differentiate station type, by touching each station Mix button.
button until the desired color appears. 2. Using the sliding scales, adjust the following settings
• Touch View to view station colors as necessary:
• Touch Reset Colors to reset to original colors. Mix Amplitude 10 to 30 mm
3. Touch Save to save color changes and exit. Mix Frequency 1 to 10 times
Mix Speed 1 to 7 (7 being the fastest)
To edit station parameters: 3. Touch Save to save changes.
1. From the Station Properties screen, touch Station • Touch Restore Defaults to reset.
Parameters.
NOTE: This will restore all default system parameters.
• The Station Parameters screen displays.
• Touch Exit to exit without saving any changes.
Revised 3/30/2010 3.17

To change enhanced wash settings To adjust basket lifting speed and water wash cycle
time:
1. From the Station Parameters screen, touch the
Enhanced Wash button. 1. From the Station Parameters screen, touch the
• The following screen displays: Basket Lifting Speed button.
2. Using the sliding scales, adjust the following settings

as necessary: 2. Using the sliding scale, adjust basket lifting speed
Enhanced Wash Frequency 1 to 10 times from 1 to 6 (6 being fastest).
Enhanced Wash Speed 1 to 5 (5 being the • This screen also lists Water Wash Cycle Time.
fastest) To adjust:
3. Touch Save to save changes. 3. Touch the Modify button and set water wash cycle
time from 30 to 90 seconds.
• Touch Restore Defaults to reset.
4. Touch Save to save changes.
NOTE: This will restore all default system settings for the
• Touch Restore Defaults to reset.
station parameters.
• Touch Exit to exit without saving any changes. NOTE: This will restore all default system parameters.
• Touch Exit to exit without saving any changes.
3.18 Revised 3/30/2010

Section 3
2. Adjust the following Drying and Heating Station

To adjust temperature settings:
parameters as necessary:
1. From the Station Parameters screen, touch the Drying Station Temperature:
Temperature Settings button.
1. Touch Modify and change the temperature with the
• The following screen displays: range of 30 to 65˚ C.
2. Touch Save.
Heating Station 1 Temperature
1. Touch Modify and change the temperature within the
range of 30 to 70˚ C
2. Touch Save.
Heating Station 2 Temperature
1. Touch Modify and change the temperature within the
range of 30 to 70 C.
2. Touch Save.
Revised 3/30/2010 3.19

Programming the Tissue-Tek Prisma
Programs are used to instruct the Tissue-Tek Prisma
which solution configurations should run in which order.
Programs can be added, copied, and exported. Up to 50
programs can be stored on the system.
Adding New Programs

1. From the Edit Menu, touch the Edit Program button.
• The Edit Program screen displays.
3. From the Solution Configuration List, touch to

highlight the solution configuration to be used for this
program and then touch Select.
• The Create Program Name screen displays.
The solution configuration may need to be changed to

bring up a new program list. Touch Change Solution
Configuration and select the desired configuration.
2. To add From the Edit Program screen, touch the Add
Program button.
• The Select Solution Configuration dialog box
displays.
4. Using the touch keyboard, enter a program name, up

to 20 characters long and then touch Save.
• The Create Abbreviated Program Name screen
displays.
3.20 Revised 3/30/2010

Section 3
Copying Existing Programs

Existing programs can be used as a template to create
a new program. When a program is copied, program steps
and settings are duplicated to create a new program.
1. From the Edit Menu, touch the Edit Program button.
• The Edit Program screen displays.
2. From the Program List, select to highlight the program
to be copied and then touch the Copy button.
• The Copy Program screen displays.
3. From the Copy To list, select to highlight the program
to copy the information to or touch Add Program to
add a new program to the list.
5. Using the touch keyboard, enter an abbreviated Modifying Program Steps

program name, up to 5 characters long, and then
touch Save. This is the name that displays in the Program steps are modified using the Edit Program
overview monitor screen (stain in process). 早Program Name旨 screen.
• The Edit Program “Program Name” Screen This screen displays immediately after a new program
displays. This screen allows the authorized user is added or can be accessed as follows:
to insert, delete, copy, paste and replace all 1. From the Edit Menu, touch the Edit Program button.
steps within a given program. • The Edit Program screen displays.
to be modified.
• The Edit Program “Program Name” screen
displays.
6. Create program steps, see paragraph Modifying

Program Steps on page 3.21.
Revised 3/30/2010 3.21

• The Enter Time dialog box displays.
Setting Solution Station
1. Touch to highlight the Station field in the step to be
modified and then touch Select Station.
Note: If the wrong step was selected use the up and

down arrows to position the cursor to the desired field.
3. Use the touch keypad to select hours, minutes and
seconds and adjust accordingly.
2. Touch the solution station to be assigned to this step
4. Touch Enter.
and then touch Select.
• Touch Clear to erase the settings.
Setting Process Time • Touch Exit to exit without saving.
Note: At least one step must have a programmed Setting Delay Time
process time.
1. Touch to highlight the Delay field in the step to be
1. Touch to highlight Time field in the step to be
modified.
modified.
2. Select delay time of Exact, Limited 20%, Limited

2. Touch the Modify button.
50% or Unlimited using the appropriate button.
3.22 Revised 3/30/2010

Section 3
Note: If the wrong step was selected use the up and To delete a step
down arrows to position the cursor to the desired field.
• Touch the screen to select and highlight the step
3. Touch Save. to be deleted and then touch Delete Step.
Setting Mix Function Confirming Program Changes
1. Touch to highlight the Mix field in the step to be When all changes have been made to the selected
modified. program:
• The following screen displays: 1. Touch Save.
• A Confirmation dialog box displays.
2. Select Mix functionality of Mix (ON), Enhanced

Wash, or OFF using the appropriate button.
2. Touch Save and Exit to save program changes and
3. Touch Save. return to the Edit Menu.
To copy a program step • Touch Exit to exit without saving changes
1. Touch to highlight the Step field of the step to be • Touch Continue to continue editing the selected
copied. program.
2. Touch Copy.
3. Touch to highlight the Step field below where the
copied step is to be pasted and touch Paste.
4. Touch Save.
To insert a new step:
1. Touch the screen to select the step below where the
new step is to be inserted and touch Insert Step.
2. Step numbers are added automatically.
Revised 3/30/2010 3.23

Deleting Data
Solution names, solution configurations, programs and
process reports can be deleted from the system.
1. From the Edit Menu, touch the Delete Data button.
• The Delete Data screen displays
3. Touch Yes to confirm deletion or No to cancel the

delete procedure.
To delete a preset program:
4. From the Delete Data screen, touch Program.
• A Deletion Procedure screen displays.
To delete a solution configuration:
1. From the Delete Data screen, touch Solution
Configuration.
• A Deletion Procedure screen displays.

to be deleted, or touch the All Data button to delete
all programs.
2. From the Solution Configuration List, select to 6. Touch Yes to confirm deletion or No to cancel the
highlight the configuration to be deleted, or touch the delete procedure.
All Data button to delete all solution configurations.
3.24 Revised 3/30/2010

Section 3
To delete a solution name: Checking Program Validity

1. From the Delete Data screen, touch Program. The Check Program button on the Edit Menu allows the
user to check the compatibility of a staining program with
• A Deletion Procedure screen displays. a solution configuration.
To check program compatibility:
1. From the Edit Menu, touch the Program Check
button.
• The Program Check screen displays.
2. From the Solution List, select to highlight the solution

to be deleted, or touch the All Data button to delete
all programs.
delete procedure. 2. From the Program List, select to highlight the program
To delete a process report to check.
3. From the Solution Configuration List, select to
1. From the Delete Data screen, touch Process Report. highlight the configuration to check against the
• A Confirmation dialog box displays. selected program.
4. Touch the Check button.
• The Program Check Results screen displays.
• If the program is compatible a list of stations and
solutions appears.

delete procedure.
Revised 3/30/2010 3.25

5. Press Exit to return to the Program Check screen.
3.26 Revised 3/30/2010

Section 4
OPERATING INSTRUCTIONS
Initial Setup Preparation
Ensure that the initial setup procedures have been CAUTION: The use of iodine on this instrument may
performed in accordance with the requirements of Section cause oxidation on some parts. It is recommended
3, Customization of Settings, prior to operating the Tissue- that if iodine must be used in some staining methods,
Tek® Prisma®. that it be removed immediately upon completion of
the stain. Failure to remove the iodine may cause
oxidation.
Understanding the Control Panel
Display Loading of slides
The control panel provides the controls and indicators
Slides must be correctly inserted in the slide baskets
necessary to initiate a program, monitor the status of
prior to loading them on the instrument. The following
processes currently running, and configure system
precautions must be followed:
settings.
• The specimen side of the slide must face forward
(towards the words “upside” on the basket).
Process Monitor Screen • The label end of the slide must be outside (top)
The Process Monitor Screen (Figure 4-A) is displayed of the basket.
when the system is ready for operation. It shows the • Each slide must be in parallel grooves
current solution configuration and allows for log on and to
• Each basket holds 20 slides; the empty grooves
monitor any processes currently running.
in a partially filled basket may be anywhere in the
basket.
Before starting a program, verify the following:
• The water supply and drain hose are securely
connected (see Section 2, Installation) and the
water run up time is set according to the
procedure in Section 3.
• All solution reservoirs are installed and filled with
the appropriate solutions.
• Dryer stations, wash stations, and start/end
stations are properly installed (see Section 2)
• Carbon filters have been installed (see section 2)
Figure 4-A
Revised 3/30/2010 4.1

Accessing the Main Menu
Note: You must login as an Administrator in order to
modify system settings and operating parameters.
To access the Menu:
1. From the main system (Process Monitor) screen,
touch the Log On button. The Enter Password dialog
box displays.
2. Use the touch keypad to enter your password, then
touch the Enter button.
• The default password is set to “100000”.
Note: For security reasons, your password will display on
the screen, as entered, as a series of asterisks.
The Tissue-Tek Prisma Menu consists of three tabbed
• If your login is unsuccessful: Re-enter your pages that provide access to the following submenus
password and then press the Enter button to Utility Menu, Edit Menu, and Stain Process Menu.
resubmit your login request. System configuration and setup are performed from the
• If your login is successful: The Stain Process Utility and Edit menus and are described in Section 3,
Monitor screen displays. Customization of Settings. The Stain Process Menu is
used for system operation and is described below.
Stain Process Menu

The following options are available from the Stain
Process Menu.
Utility Menu
Solution Configuration Selection –
allows the user to choose and view
programmed solution configurations.
Staining Mode Selection – allows the

user to change and view programmed
staining modes.
3. Touch the Menu button to display the main menu. Reset Solution Usage Information –
allows the user to reset usage information
Error Log – allows the user to view a log

of system errors.
4.2 Revised 3/30/2010

Section 4
Solution Configuration Selection

This option allows the user to choose and view
programmed solution configurations. (See solution
configurations setup in Section 3.)
1. From the Stain Process Menu, touch the Solution
Configuration Selection button.
• The Solution Configuration Selection screen
displays.
• Touch No to cancel the selection and return to

the Solution Configuration Selection screen.
4. Touch Yes to accept the new solution configuration
and display the New Solutions Configuration screen.
2. Touch to select a solution configuration from the list

and then select from the following options or proceed
to step 3.
• Touch View to view the selected program
• Touch Check Program to view all compatible
programs.
3. Touch Select to complete selection. The following
confirmation dialog box displays:
• Touch Change View to toggle between left and

right to view all stations.
• Touch Export Data to export solution
configuration selection data to a compact flash or
printer.
• Touch Exit to return to the previous screen.
5. Touch Select to accept the selected solution
configuration.
NOTE: A solution configuration change may require the
3-basket arm holder to be replaced with the 1-basket arm
holder. Change the arm if necessary and answer Yes if
changed.
Revised 3/30/2010 4.3

Staining Mode Selection
The Stain Mode Selection option from the Stain
Process Menu allows the user to predetermine start
options and process mode.
1. From the Stain Process Menu, touch the Staining
Mode Selection button.
• The Staining Mode Selection screen displays.
To change the basket holder

NOTE: The three-arm basket holder will need to be
removed to accommodate the expansion or special stain
configurations.
NOTE: If the three-arm basket adapter is not removed,
the basket may become misaligned resulting in a robotic
arm malfunction.
• Press in the black lever at the top of the basket The Staining Mode Selection screen presents two
holder. Grasp the top of the 3-arm basket holder tabbed pages, Staining Mode and System Setup that
and gently slide upwards to remove. The basket allow the user to set staining mode options and
adapter may need to be modified depending on determine certain system options.
the type of solution configuration used. The 3-
2. Touch the Staining Mode tab and make changes to
arm basket holder will need to be removed to
the following settings as necessary.
accommodate the expansion or special stain
configurations. • Staining Mode Selection – Select either Batch
Mode or Continuous Mode. Batch Mode moves
one basket set through the entire staining
process before beginning another basket set.
Continuous mode allows the continuous addition
of basket sets as long as a start station is open
• Initialization of Start Staining Screen - This
option is used for initiating the start screen. There
are two options:
Touching START button after closing the door
Closing the door
• Initialization of Staining Process Screen - This
function refers to the physical action of starting
6. Touch Exit to return to the Stain Process Menu. the stain process. There are three options:
Touching START button
Touching START button after closing the door.
Closing the door
4.4 Revised 3/30/2010

Section 4
• Start Confirmation - Confirms the start action. Reset Solution Usage Information
There are two options: This function allows users to reset the solution stations
In Use that have reached their usage limit. (See section 3 for
Not In Use setting reagent management features)
• Process Start Notice - Reconfirmation that the 1. From the Stain Process Menu, touch the Reset
process has started. There are two options: Solutions Usage Information button.
In Use • The Reset Solution Usage Information screen
Not in Use displays.
3. Touch the System Setup Tab and make changes to
the following settings as necessary.
• Heating Station Always Heated - When the unit
is powered on, the heating stations are always
on. There are two options:
Yes
No
NOTE: This option is only available on product # 6131-
Tissue-Tek Prisma with Special Stains.
• Solution Name Display - This allows the user to
view the solution names on the Stain Process
Monitor Screen. The options are:
Yes
No
• Link with Coverslipper - If the Prisma® is
• To select a specific station touch the station
connected to the Film® Coverslipper there is a
number.
choice to have the instrument transfer the slides
automatically or the instrument can be used • To select the stations that have reached their
independently. Options are: usage limit, touch Overused Stations.
Yes • To select all stations, touch All Stations.
No 2. Once stations have been selected, touch Reset to
Note: This option is only available if the Prisma is reset values to zero.
connected with the Tissue-Tek® Film® Coverslipper. • A confirmation dialog box appears, answer Yes
4. Touch Exit to return to the Stain Process Menu. to reset the solution usage information.
3. Touch Exit to return to the Stain Process Menu.
Revised 3/30/2010 4.5

Error Log Starting a Stain Process
This function allows the user to view the error log.
1. Log on to access the main, Stain Process Monitor,
1. From the Stain Process Menu, touch the Error Log
menu.
button.
• The Error Log screen displays.
2. Touch Start to initiate the Start screen.

•Touch Error Description for information about
the all errors that have occurred.
• Touch Clear to clear all errors listed.
A confirmation window will appear, answer Yes
to delete the Error Log.
2. Touch Exit to return to the Stain Process Menu.
NOTE: Start confirmation may be different depending on

the settings programmed under Staining Mode Selection,
refer to page 4.3.
4.6 Revised 3/30/2010

Section 4
3. From the list, select a desired program. Monitoring a Staining Process

4. Place appropriately loaded baskets into any available
start stations. Baskets are loaded with the “upside” on During the staining process, the Stain Process Monitor
the basket adapter facing the right side of the Screen is displayed. The Stain Process Monitor is a visual
instrument. display that identifies where all staining runs are located at
any given time. The abbreviated name of the staining
NOTE: If starting more than one basket set, place process will be displayed in the solution station that is
baskets in an available start station and touch another of currently in progress. From the Stain Process Monitor
the unoccupied start stations on the screen. A basket screen the operator can make various selections.
mark will appear in the loaded start stations. Additional baskets may be added, detailed monitor
5. Close the door or touch Start to initiate the start checked, end station status checked, baskets removed,
process. and runs aborted.
NOTE: Initiation of the Start is dependent on the settings
programmed under Staining Mode Selection. If the touch
Start option is used, the Start can be touched only after
the door is opened and closed. It is otherwise grayed out
and cannot be selected.
• If the Start confirmation option is programmed,
touch Start when the window appears.
• Touch Cancel if desired start needs to be
cancelled. Remove baskets if necessary and
touch Exit.
The Stain Process Confirmation will appear if programmed
to do so. Touch Exit to remove the window otherwise it
will automatically disappear after a certain period of time.
Revised 3/30/2010 4.7

Adding Baskets • This displays the Detailed Monitor screen. The
Additional baskets may be added during the staining status of the current stain process and the
process. predicted end times are displayed.
1. From the Stain Process Monitor screen, touch the
Add Baskets button.
2. Select a desired program from the program list.
3. Place appropriately loaded baskets into any available
start stations. Baskets are loaded with the “upside” on
the basket adapter facing the right side of the
instrument.
NOTE: If starting more than one basket set, place baskets
in an available start station and touch another of the
unoccupied start stations on the screen. A basket mark
will appear in the loaded start stations.
4. Close the door or touch Start to initiate the start
process.
• Touch Overview Monitor to return to the Stain

NOTE: Initiation of the Start is dependent on the settings
Process Monitor Screen.
programmed under Staining Mode Selection. If the touch
[Start] option is used, the [Start] can be touched only after
the door is opened and closed. It is otherwise grayed out Monitoring the End Station Status
and cannot be selected.
At any time during a staining run, the end station status
• If the Start confirmation option is programmed, can be monitored.
touch Start when the window appears.
• Touch Cancel if desired start needs to be Detailed Monitor button.
cancelled. Remove baskets if necessary and
2. Touch the End Station Status. The status of each
touch Exit.
end station is displayed.
The Stain Process Confirmation will appear if programmed
to do so. Touch Exit to remove the window otherwise it
will automatically disappear after a certain period of time.
Detailed Monitor
While a staining run is in progress, the operator can
check the time left for a particular run and get an
estimated time for completion. The Detailed Monitor
Screen will display the run number for each program
currently running, the status of that run, the step and
station currently in, the time left in that station, and the
predicted end time for that run.
Detailed Monitor button.
3. Touch the Exit button to return to the Stain Process
Monitor Screen.
4.8 Revised 3/30/2010

Section 4
Removing Baskets To interrupt a staining process:

After a run has been completed and the baskets are 1. From the Stain Process Monitor Screen touch Pause.
positioned in the end stations or Programmed End station, (If the robotic arm is moving, the message, ‘Please
the baskets can be removed. The Remove Basket button Wait’, will appear. When the robotic arm has stopped,
will be grayed out and will not function if there are no the On Hold window will be displayed). From this
baskets to be removed. window the operator has two options: touch Resume
1. From the Stain Process Monitor screen, touch the to return to the Stain Process Monitor Screen or touch
Detailed Monitor button. Abort to cancel a staining run.
2. Touch the Remove Basket button.
2. From the Abort Stain Process Screen, touch one or

3. Open the door and remove baskets from the indicated more runs to be aborted, or touch All Runs to abort
end stations. all runs.
4. If the baskets have been removed from the indicated • Touch Abort to abort.
end stations, touch Yes to confirm their removal. • Touch Resume to resume the staining process.
• Touch Yes to confirm the aborted staining
Interrupting or Canceling a Staining process.
Process
From the Stain Process Monitor Screen, the operator
may choose to interrupt or cancel a staining run. During
process interruption, the robotic arm will not move. The
interruption should be as short as possible to avoid
affecting stain quality if multiple staining programs are in
progress.
Revised 3/30/2010 4.9

Finishing the Stain Process
When the stain process is completed the alarm will
sound to inform the operator that the stain process has
been completed. The Stain Process Completed Window
will be displayed.
3. Open the cover and remove all baskets from the

indicated stations.
1. Remove all baskets from the indicated End Stations.

2. Touch the Exit button
NOTE: The Exit button will not become active until the
cover is opened and then closed.
3. Touch Yes to confirm that the baskets have been
removed
NOTE: If the Tissue-Tek® Prisma® is connected to the
Tissue-Tek® Film® Coverslipper, the baskets will be
automatically transferred for coverslipping.
4. Touch Exit when baskets have been removed.

NOTE: The Exit button will not become active until the
cover is opened and then closed.
4.10 Revised 3/30/2010

Section 5
ACCESSORIES
Standard Accessories
Basic Unit
Product Product
Code Description Code Description
4768 20-Slide Basket

10-Slide Basket
6139 Load/Unload
Adapter*
20-Slide Basket
6135 Load/Unload
Adapter Special Stain
6140 Solution Reservoir,
160 ml*
20-Slide Basket Special Stain

6136 6144
Adapter Reservoir Lid*
Standard Solution
6147 Reservoir,
680 ml
6137 10-Slide Basket*
6138 10-Slide Basket Load/Unload

Adapter* 6148 Reservoir with
Handle
Revised 3/30/2010 5.1

ACCESSORIES
Product Product
Code Description Code Description
Wash Reservoirs Activated Carbon

6149 6160
850 ml Filter
Station Labels (Start,

6161 No image available
End, PE)
6162 Basket Adapter Label No image available
Standard Solution Control Panel
6151 6163 No image available
Reservoir Lid Protection Sheet
6164 Compact Flash Card No image available
* for product 6131 only
3-Position
6152
Reservoir Tray
3-Position Reservoir
6153
Lid
6154
Tray
6155
Lid
Special Stain
6156
Reservoir Tray*
5.2 Revised 3/30/2010

Section 5
Optional Accessories
Product
Code Description
Small Solution
6145 Reservoir,
260 ml
Small Solution
6146
Reservoir Lid
Duct Connection
6165 Adapter,
38mm dia.
Duct Connection
6166 Adapter,
75mm dia
Revised 3/30/2010 5.3

ACCESSORIES
5.4 Revised 3/30/2010

Section 6
CARE OF THE INSTRUMENT

General Maintenance
Keep the exterior of the Tissue-Tek® Prisma®
Automated Slide Stainer free from dust. Do not use
solvents of any kind on the control panel. The plastic
cover may be cleaned with a glass cleaner and a soft
cloth.
Daily Maintenance
Exterior/Interior Surfaces
Figure 6-A
Daily clean the plastic and painted areas by wiping with
a clean cloth. Clean the interior of the solution area by 3. Lower the filter cover.
wiping with a clean cloth. 4. Remove the plastic wrapping from both carbon filters.
5. Remove the activated carbon filters from the
Bi-Monthly Maintenance instrument and dispose of them properly.
6. Place the carbon filters side by side in a horizontal
Replacement of the Carbon Filter position in the appropriate location (Figure 6-B).
It is recommended that the carbon filters be changed
after two weeks or sooner if fume monitoring reveals
elevated reagent vapors.
Keep the filters in place even if there is an internal duct
system attached.
To replace the carbon filters:
CAUTION: Make sure that the instrument is switched
off and the power cord is unplugged before
beginning.
1. Open the main cover of the instrument and locate the
Figure 6-B
filter cover located on the rear panel inside of the
instrument. 7. Push firmly against the filter cover to close and latch
2. Open the filter covers by pressing down on the button the hook.
located at the top center of the filter cover (Figure 8. Lower the main cover.
6-A).
Revised 3/30/2010 6.1

CARE OF THE INSTRUMENT
Monthly Maintenance Reservoir Trays
Drying Station The reservoir trays should be cleaned once a month.
1. Turn the power off by pressing the power switch to
The drying stations should be cleaned once a month or
the “O” position.
more often as needed.
2. Open the Main Cover
1. Turn the power off by pressing the power switch to
the “O” position. 3. Remove the reservoir trays from the instrument by
grasping on to the handles located on each side of
2. Open the main cover.
the tray (Figure 6-C).
3. Wait until the temperature of the drying stations has
reached a comfortable level, and remove the inner
components of the drying stations.
4. Remove the residue and any debris from the
components by washing with water and air-drying.
Return components to the inside of the drying stations
and verify proper insertion.
Solution Reservoirs and Slide Baskets

The solution reservoirs and slide baskets should be
washed with tap water or a mild detergent once a month.
If stains and residue are difficult to remove from dried Figure 6-C
reagents, soak first in a commercially available chemical 4. Remove the solution reservoirs from the reservoir
cleaning detergent or bleach solution and then rinse with a trays.
mild detergent and water. Cleaning reservoirs and slide
5. Remove the residue and any debris from the trays by
baskets monthly will help to keep these items free from
washing with water or mild detergent and let air-dry.
permanent stains. Always use the same reservoir for a
given solution to avoid contamination. 6. Return the solution reservoirs to the original positions
and place the reservoir trays back into the instrument.
7. Close the Main Cover.
6.2 Revised 3/30/2010

Section 7
TROUBLESHOOTING
General Information If addition assistance is required concerning an
instrument problem, or if the problem cannot be isolated or
The following Troubleshooting Chart contains general is beyond the scope of this manual, please contact the
problems that could occur during operation of the Tissue- Sakura Finetek USA, Technical Support Department. If
Tek® Prisma® Automated Slide Stainer. Probably causes outside the USA, call the nearest Sakura representative.
and recommended remedies are included for each
problem listed, to aid in diagnosis and correction.
Revised 3/30/2010 7.1

TROUBLESHOOTING
Message Description Function Recovery Action

System Error A system error has occurred. Stop by pressing the On/Off Switch. Turn instrument power off and contact
(Emergency Stop). The staining process will Sakura Technical Support.
be interrupted.
Power Outage A Power outage has occurred Arm will return to the home position after the Perform recovery process (continue or
power is restored abort run).
Power Outage Recovery Detected inconsistent or abnormal Power failure recovery of staining process is Repeat staining process.
Failure data at the time of memory check aborted
during power on.
Cover Open Hood was open during staining Emergency stop condition Close hood to continue process.
process.
Cover Open (not during Hood was open when arm was Emergency stop condition. Close hood to continue process.
staining moving but not during staining
process.
Processing Halt A temporary stop by the user. Although this is handled as an error, it is not Restart operation.
an abnormal condition. Error is logged into
the history.
Door Open Door was open when the robotic Emergency stop condition. Close door to continue process.
arm was moving to front row
reservoirs.
Printer Error A printer error has occurred. Message display only. Change printer settings to “print” and
restart the printing operation.
Exhaust Fan Stop The exhaust fan has stopped. Message display only. Turn instrument power off and on.
Check to see if fan has restarted. If not
contact Sakura Technical Support
Department.
Battery Voltage Low Voltage of back-up battery is low. Message display only. When the battery voltage becomes low
the memory cannot be backed up and
recorded information will be lost.
Contact Sakura Technical Support
Department.
Motor Unit Communication Serial communication failure Abort staining process. Turn instrument power off and on.
Error between operation panel CPU Check robotic arm function. If error
board and control board CPU2. reoccurs, contact Sakura Technical
Support Department.
I/O Unit Communication Serial communication failure Abort staining process. Turn instrument power off and on. If
Error between operation panel CPU error reoccurs during startup, contact
board and control board CPU1. Sakura Technical Support Department.
Vacuum Breaker Error A vacuum breaker error has Abort staining process. Contact Sakura Technical Support
occurred. Department.
Robotic Arm Error A robotic arm error has occurred. Emergency stop condition. Perform recovery process (continue or
abort run).
Home Position Sensor X axis home position return sensor Abort staining process. Turn instrument power off and on.
Error (X axis) detected error. Check robotic arm function. If error
reoccurs, contact Sakura Technical
Support Department.
Wire Slack (X axis) Detected X axis wire slack. Abort staining process. Turn instrument power off and on.
Check robotic arm function. If error
Support Department.
7.2 Revised 3/30/2010

Section 7

Deviation Error (X axis) Detected misalignment (misstep) Emergency stop condition. Perform recovery process (continue or
of X axis robotic arm. abort run).
Wire Cut Off (X axis) Detected X axis wire derailment. Emergency stop condition. Contact Sakura Technical Support
Department.
Home Position Sensor Y axis home position return sensor Abort staining process. Turn instrument power off and on.
Error (Y axis) detected error. Check robotic arm function. If error
Support Department.
Wire Slack (Y axis) Detected Y axis wire slack. Abort staining process. Turn instrument power off and on.
Support Department.
Deviation Error (Y axis) Detected misalignment (misstep) Emergency stop condition. Perform recovery process (continue or
of Y axis robotic arm. abort run).
Wire Cut Off (Y axis) Detected Y axis wire derailment. Emergency stop condition. Contact Sakura Technical Support
Department.
Home Position Sensor Z axis home position return sensor Abort staining process. Turn instrument power off and on.
Error (Z axis) detected error. Check robotic arm function. If error
Support Department.
Wire Slack (Z axis) Detected Z axis wire slack. Abort staining process. Turn instrument power off and on.
Support Department.
Deviation Error (Z axis) Detected misalignment (misstep) Emergency stop condition. Perform recovery process (continue or
of Z axis robotic arm. abort run).
Wire Cut Off (Z axis) Detected Z axis wire derailment. Emergency stop condition. Contact Sakura Technical Support
Department.
Sensor Opened (Drying Drying Station A sensor is Temperature control at drying station A is Stop processes using drying stations.
Station A) disconnected. stopped. All other functions are running Contact Sakura Technical Support
normally. Department.
Sensor Shorted (Drying Drying Station A sensor has short- Temperature control at drying station A is Stop processes using drying stations.
Station A) circuited. stopped. All other functions are running Contact Sakura Technical Support
Temperature Low (Drying Temperature has not reached the Temperature control at drying station A is Stop processes using drying stations.
Station A) set temperature at Drying Station stopped. All other functions are running Contact Sakura Technical Support
A in the allotted time. normally. Department.
Thermal Fuse (Drying Internal temperature of Drying Temperature control at drying station A is Stop processes using drying stations.
Station A) Station A has reached 85º C. The stopped. All other functions are running Contact Sakura Technical Support
over-temperature prevention normally. Department.
function is running.
Fan Stop (Drying Station Drying Station A fan has stopped Temperature control at drying station A is Stop processes using drying stations.
A) functioning. stopped. All other functions are running Contact Sakura Technical Support
Sensor Opened (Drying Drying Station B sensor is Temperature control at drying station B is Stop processes using drying stations.
Station B) disconnected. stopped. All other functions are running Contact Sakura Technical Support
Sensor Shorted (Drying Drying Station B sensor has short- Temperature control at drying station B is Stop processes using drying stations.
Station B) circuited. stopped. All other functions are running Contact Sakura Technical Support
Revised 3/30/2010 7.3

TROUBLESHOOTING
Temperature Low (Drying Temperature has not reached the Temperature control at drying station B is Stop processes using drying stations.
Station B) set temperature at Drying Station stopped. All other functions are running Contact Sakura Technical Support
B in the allotted time. normally. Department.
Thermal Fuse (Drying Internal temperature of Drying Temperature control at drying station B is Stop processes using drying stations.
Station B) Station B has reached 85º C. The stopped. All other functions are running Contact Sakura Technical Support
over-temperature prevention normally. Department.
Fan Stop (Drying Station Drying Station B fan has stopped Temperature control at drying station B is Stop processes using drying stations.
B) functioning. stopped. All other functions are running Contact Sakura Technical Support
Sensor Opened (Heating Heating Station A sensor is Temperature control at Heating Station A is Stop processes using Heating
Station A) disconnected. stopped. All other functions are running Stations. Contact Sakura Technical
normally. Support Department.
Sensor Shorted (Heating Heating Station A sensor has Temperature control at Heating Station A is Stop processes using Heating
Station A) short-circuited. stopped. All other functions are running Stations. Contact Sakura Technical
Temperature Low (Heating Temperature has not reached the Temperature control at Heating Station A is Stop processes using Heating
Station A) set temperature at Heating Station stopped. All other functions are running Stations. Contact Sakura Technical
A in the allotted time. normally. Support Department.
Thermal Fuse (Heating Internal temperature of Heating Temperature control at Heating Station A is Stop processes using Heating
Station A) Station A has reached 85º C. The stopped. All other functions are running Stations. Contact Sakura Technical
over-temperature prevention normally. Support Department.
Sensor Opened (Heating Heating Station B sensor is Temperature control at Heating Station B is Stop processes using Heating
Station B) disconnected. stopped. All other functions are running Stations. Contact Sakura Technical
Sensor Shorted (Heating Heating Station B sensor has Temperature control at Heating Station B is Stop processes using Heating
Station A) short-circuited. stopped. All other functions are running Stations. Contact Sakura Technical
Temperature Low (Heating Temperature has not reached the Temperature control at Heating Station B is Stop processes using Heating
Station A) set temperature at Heating Station stopped. All other functions are running Stations. Contact Sakura Technical
B in the allotted time. normally. Support Department.
Thermal Fuse (Heating Internal temperature of Heating Temperature control at Heating Station B is Stop processes using Heating
Station A) Station B has reached 85º C. The stopped. All other functions are running Stations. Contact Sakura Technical
over-temperature prevention normally. Support Department.
No Communication With Settings show that the Film and Communication between the Prisma and Check to see if the Film is ready for
Film Coverslipper Prisma should be connected but Film cannot be established. use or communication cable between
communication cannot be reached. the Prisma and Film is correctly
connected.
Film Operation Stop Hood was open during operation Slides will be transferred to and End Station Close hood and perform recovery
and could not transfer the Film until the Film loading station is available. process; reestablish communication
loading station to the Prisma. with Film.
File System Error (A Drive) Memory card in the A Drive is Data cannot be transferred into memory Use another readable/writeable
unrecognizable. card in the A Drive. All functions including memory card.
staining processes are usable.
File Not Found (A Drive) Failed to detect file in A Drive. Data cannot be transferred into memory Insert memory card in the A Drive. If
card in the A Drive. All functions including error still occurs, use another
staining processes are usable. readable/writeable memory card.
No Space in Memory Card Shortage of memory card capacity Data cannot be transferred into memory Replace the memory card in A Drive.
7.4 Revised 3/30/2010

Section 7

(A Drive) in A Drive. card in the A Drive. All functions including
Write-protect Error (A Detected a write-protection error Data cannot be transferred into memory Release the write-protection under A
Drive) with the memory card in A Drive. card in the A Drive. All functions including Drive.
File System Error (B Drive) Memory card in the B Drive is Instrument functions with control information Change settings to use the
unrecognizable. that was initially stored. All functions instrument's initial values for
including staining processes are usable. controlling.
File Not Found (B Drive) Error detected with memory for Instrument functions based on control Restore information settings.
control during its read/write of file information of memory. All functions
in B Drive. including staining processes are usable.
Wrong password. Reenter Password entered does not match Suspends password input if password is Reenter (restore) information under
password with the one registered in the entered 3 times. setting operation.
instrument.
No program to perform. There is not a usable staining Message display only. Change Station Configuration to the
program with the current station desired program. Remove or insert
configuration when pressing the Special Stain Reservoir Tray
"Start Staining" button. When accordingly.
"Start Staining button is pressed,
the current station configuration is
set for special stains without
having the special stain reservoir
tray set. When "Start Staining"
button is pressed, the current
station configuration in NOT set for
special stains with the special stain
reservoir tray set.
Please set 10-Slide Basket Staining program requires the 10- Message display only. Set the 10-Slide Basket Adapter in the
Adapter. Slilde Basket; the 10-slide Basket Load Stations and use the 10-Slide
Adapter is not placed into the Basket.
instrument.
Door is Open Staining process cannot be started Message display only. Close the door and start the staining
because the door is open. process.
Cover (Hood) is Open Staining process cannot be started Message display only. Close the Hood and start the staining
because the Hood is open. process.
Station Configuration When "Start Staining" button is Message display only. Set Special Stain Reservoir Tray in the
shows Special Stains, but pressed, the current station instrument.
the special stain solution configuration is not set for special
reservoir tray is not set. stains but the special stain
reservoir tray is not set.
Station Configuration does When "Start Staining" button is Message display only. Remove the Special Stain Reservoir
not show special stains but pressed, the current station Tray and replace it with the Standard
the special stain solution configuration is not for special Solution Reservoir Tray.
reservoir is set. stains but the special stain solution
reservoir is set.
Cover (Hood) is Open. Cover (Hood) was opened during Emergency stop condition. Close the cover and perform recovery
Staining process is the staining process and operation.
stopped. processes have stopped.
Cover (Hood) is Open. Cover (Hood) was opened during Emergency stop condition. Close the cover and perform recovery
Robotic arm has stopped. the staining process and the operation.
robotic arm has stopped.
Door is Open. Staining Door was opened when the robotic Emergency stop condition. Close the door and perform recovery
Revised 3/30/2010 7.5

TROUBLESHOOTING
process is stopped. arm was moving to the front row operation.
reservoirs.
Robotic Arm cannot be Due to an emergency stop during Emergency stop condition. Perform recovery operation.
moved due to an a basket transfer by the robotic
emergency stop. arm, the instrument could not
judge if the robotic arm could be
moved during the recovery
process.
End Station is full. End Station or P/E station is/are Processes that cannot continue will stop. Remove basket(s) promptly from the
full and the processes in progress Processes that can will continue. end stations and/or P/E Station.
cannot be continued.
The reagent or filter has Solution under management All functions are usable including staining Replace reagents or filters exceeding
exceeded its usage limit. method has exceeded the set time process. the usage limit.
(Days or Runs). When managing
filter usage, set time has been
exceeded.
Name is duplicated. Solution Name already exists. Message display only. Select other unused name for solution.
Station Configuration Name Select other unused name for station
already exists. Staining Program configuration. Select other unused
Name already exists. name for staining program.
Programs cannot be There is not step to process when Message display only. Revise staining program.
registered. There is no executing staining process and
step with designated staining program was not
processing time. registered.
Programs cannot be There is a step that cannot be Message display only. Revise staining program.
registered. There is no performed when executing staining
step with designated process and staining program was
processing time. not registered.
Password duplication Password entered has already Message display only. Input a number that is not already
(password already exists) been registered as a password. registered.
7.6 Revised 3/30/2010

Section 8
SERVICE AND REPLACEMENT PARTS

Service Information Replacement Accessory Items
For All Models
When a Problem Occurs Product Number Description
4768 20-Slide Basket
When a problem occurs during operation of the Tissue-
6135 20-Slide Basket Load/Unload Adapter
Tek® Prisma® Automated Slide Stainer, refer to Section 7,
Troubleshooting, to determine the most likely cause of the 6136 20-Slide Basket Adapter
malfunction and to obtain recommended corrective 6137* 10-Slide Basket
actions. (Avoid problems by carefully following the proper 6138* 10-Slide Basket Adapter
operation and maintenance procedures described in this 6139* 10-Slide Basket Load/Unload Adapter
manual). If the problem cannot be solved and an 6140* Special Stain Solution Reservoir, 160 ml
instrument failure is apparent, our Technical Support 6141* Heated Solution Reservoir
Department is available to assist you. 6144* Special Stain Reservoir Lid
Before calling for instrument service, please have 6147 Standard Solution Reservoir,
model number, installation date, and warranty period 680 ml
ready for our Technical Support Department 6148 Load/Unload Reservoirs w/ handle
Representative. For your convenience and reference, 6149 Wash Reservoirs, 850 ml
record this information in the blanks below. 6152 3-Position Reservoir Tray
Slide Stainer Model Number _______________________ 6153 3-Position Reservoir Lid
Slide Stainer Serial Number ________________________ 6154 4-Position Reservoir Tray
Installation Date _________________________________ 6155 4-Position Reservoir Lid
6156* Special Stain Reservoir Tray
Warranty Period _________________________________
6160 Activated Carbon Filter
6161 Station Labels (Start, End, PE)
Where to Call for Service 6162 Basket Adapter Label
If located within the United States, contact the 6163 Control Panel Protection Sheet
Technical Support Department of Sakura Finetek U.S.A., 6164 Compact Flash Card
Inc. by calling toll free: * Available for product #6131 only
(800) 725-8723 - Menu Option 2
In countries other than the United States, contact the
Optional Accessory Items
nearest authorized Sakura instrument distributor or For All Models
representative for service information and assistance. Product Number Description
6134 Tissue-Tek Film Link System
6145 Small Solution Reservoir, 260 ml
6165 Duct Connection Adapter,
38 mm dia.
6166 Duct Connection Adapter,
75 mm dia.
Revised 3/30/2010 8.1

SERVICE AND REPLACEMENT PARTS
8.2 Revised 3/30/2010

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© 2010 Sakura Finetek USA, Inc.

All Rights Reserved

3. CUSTOMIZATION OF SETTINGS 3.1

4. OPERATING INSTRUCTIONS 4.1

6. CARE OF THE INSTRUMENT 6.1

8. SERVICE AND REPLACEMENT PARTS 8.1

Revised 3/30/2010 1.1

1.2 Revised 3/30/2010

Revised 3/30/2010 1.3

1.4 Revised 3/30/2010

User Interface Compact Flash

Figure 1-H External Interface

Revised 3/30/2010 1.5

Drain Hose Port

1.6 Revised 3/30/2010

Power Ratings and Requirements

Revised 3/30/2010 1.7

Drain: Gravity Programs/Methods

Reservoir Capacity/Fill Volumes

*-Only for model # 6131

1.8 Revised 3/30/2010

Staining Method Names

Revised 3/30/2010 1.9

1.10 Revised 3/30/2010

Removing the Outer Shipper

Revised 3/30/2010 2.1

2.2 Revised 3/30/2010

Revised 3/30/2010 2.3

2.4 Revised 3/30/2010

Installing Solution Reservoirs

Revised 3/30/2010 2.5

2.6 Revised 3/30/2010

Heating Stations NOTE: The solution reservoir trays are configured to be

Revised 3/30/2010 2.7

2.8 Revised 3/30/2010

Electric and Link Connections

Installation of Special Stain Accessories

Revised 3/30/2010 2.9

2.10 Revised 3/30/2010

Accessing the Menu

Revised 3/30/2010 3.1

The System Setup screen allows users to adjust

3.2 Revised 3/30/2010

• Alarm Tone and Volume - sets the tone and

Setting User Names and Passwords

Revised 3/30/2010 3.3

2. Use the keypad to enter a numeric password between

To grant screen access:

3.4 Revised 3/30/2010

Revised 3/30/2010 3.5

2. Touch Clear to clear the current setting and then use

• Touch Yes to confirm that actual time is to be

3.6 Revised 3/30/2010

Creating or Changing Institution Name

2. Use the touch screen to select the language to be

Selecting System Language

Revised 3/30/2010 3.7

3.8 Revised 3/30/2010

To export solution usage status

Adjusting Water Flow