IBDP Biology SL Labs

Diffusion In Agar Cubes

Introduction:
Diffusion is one of the very important processes by which substances such as nutrients, water, oxygen, and
cellular wasted are transported between living cells and their environment. This activity will help you
explore the relationship between diffusion and cell size by experimenting with model “cells.”

In this experiment, you will use agar cubes to which the indicator phenolphthalein has been added.
Phenolphthalein is an acid/base indicator that turns pink in the presence of a base such as NaOH. Thus the
surface of the agar cubes will turn pink immediately when put into a NaOH solution. The NaOH will
continue to diffuse through the cube and gradually turn the inside of the cube pink. The guiding question
for this lab is thus:

What determines the efficiency of diffusion throughout the model “cells”? Use this question to help
formulate a hypothesis:

Your hypothesis:

Materials:
Agar infused with phenolphthalein 500ml beaker
Ruler Spoon/Tongs
0.1M NaOH Graduated cylinder
Stopwatch Paper towel
Knife

Procedure:
1. Each group will cut three agar cubes: A 3cm cube, a 2cm cube, and a 1cm cube. CUT AS
ACCURATELY AS POSSIBLE.
2. Pour 200mL of 0.1M sodium hydroxide solution into your 500ml beaker.
3. Immerse your 3 cubes in the sodium hydroxide solution, noting the start time.
4. Let the cubes soak for 10 minutes.
5. Periodically, gently stir the solution, or turn the cubes over.
6. After 10 minutes, use a spoon or tongs to remove the cubes from the sodium hydroxide solution.
 IBDP Biology SL Labs
7. Blot the with a paper towel.
8. Promptly cut each cube in half and measure the depth to which the pink color has penetrated.
Sketch each block’s cross-section. (coloured pencils are available upon request)
9. Record your measurements and sketch each cube in the table found on the next page.
10. Do the following calculations for each cube and complete the following data table:
ANALYSIS
Calculating % diffusion in each cube:
 Calculate total volume of each cube (volume = L x W x H)
 Calculate volume that did not turn pink. (That is, calculate total volume of the small portion
of the cube that did not turn pink – use the same formula L x W x H)
 Calculate volume diffused = total volume – volume not pink.
 Calculate % diffusion = Volume diffused /total volume x 100

Calculate the surface area of each cube and the surface area to volume ratio:
 Calculate the surface area of a cube = L x W x # of sides
 Calculate surface area/volume ratio.
EVALUATION
Conclusion Questions: Use these questions as the basis of your conclusion; try to use them as a
framework for your conclusion rather than actual questions. Use research and textbooks to support
your conclusion and remember to cite sources in MLA format.
Be sure to submit data table with your evaluation
1. In terms of maximizing diffusion, what was the most effective size cube that you tested?
2. Why was that size most effective at maximizing diffusion? What are the important factors that
affect how materials diffuse into cells or tissues?
3. If a large surface area is helpful to cells, why do cells not grow to be very large?
4. You have three cubes, A, B, and C. They have surface to volume ratios of 3:1, 5:2, and 4:1
respectively. Which of these cubes is going to be the most effective at maximizing diffusion, how do
you know this?
5. How does your body adapt surface area-to-volume ratios to help exchange gases?
6. Why can’t certain cells, like bacteria, get to be the size of a small fish?
7. What are the advantages of large organisms being multicellular?
 IBDP Biology SL Labs

DATA TABLE 1: Diffusion In Agar Cubes (sample table; copy into your own report)
Cube Total cube Total Sketch of each Volume of the Percent Surface area of Surface
Size volume volume Cube diffused cube Diffusion cube (cm2) area to
(cm3) that was (total volume – volume
cm
not pink volume that was ratio
(cm3) not pink)
1

Evaluation
Discuss the method by suggesting strengths and weaknesses of the investigation and suggest realistic,
relevant improvements and extensions of the investigation.

You will be assessed on Criteria for ANALYSIS and EVALUATION

Preparation of agar
1. Mix 20g of agar with one liter of distilled or deionized water. Recipe based on 15 groups of
students working in pairs.
2. Heat almost to a boil. Stir frequently until solution is clear
3. Remove from heat. AS the agar mixture cools add 10 mL of 1% phenolphthalein solution (1g
phenolphthalein in 100 mL 95% ethyl alcohol) and stir. If mixture is pink, add a few drops of
dilute hydrochloric acid until the pink color disappears.
4. Pour agar into a shallow tray to a depth of 3cm and allow it to set (over night). A tray
measuring 12cm x 2cm that is at least 3cm deep will accommodate one liter of agar mixture.