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ACT 10 - Group 4 (48 Hours Chick Embryo)

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ACTIVITY 10

The Chick Embryonic


Development in 48 Hours
Group 4 – CORUNA, GUNHURAN, HERBOLINGO, SANTIAGO
NS Embryology - Laboratory
24 Hour Chick Embryo 33 Hour Chick Embryo
ACTIVITY 10
The Chick Embryonic Development 33 to 48 Hours

Figure 1. Whole Mount of a 33 Figure 2. Whole Mount of a 48


Hour Chick Embryo Hour Chick Embryo
ACTIVITY 10
Structures of a 48 Hour Chick Embryo 1. Amnion – fluid-filled sac that encloses and
provides protection of the embryo.
2. Metencephalon – hindbrain, grows into pons
and cerebellum
3. Mesencephalon – midbrain, gives rise to
midbrain structures such as colliculi, cerebral
peduncles, and cranial nerves.
4. Optic Cup and Lens – development of the
eye structure
5. Prosencephalon – forebrain, gives rise to
endbrain (telencephalon) and diencephalon,
grows into cerebral lobes, basal ganglia,
epithalamus, hypothalamus, subthalamus, and
thalamus.
6. Otic Vesicle – auditory vesicles, two-sac like
invaginations formed and closed off during the
embryonic development specifically on the ear
structure.
Figure 3. Stained Whole Mount of a 48 Hour Chick Embryo
ACTIVITY 10 7. Branchial Arches – it consist of four essential
Structures of a 48 Hour Chick Embryo tissues such as cartilage, aortic arch artery,
nerves, and muscles.
8. Atrium – heart chamber that receives blood
from veins.
9. Ventricle – heart chamber that pumps blood out
10.Lateral Fold – skin fold on the lateral part of
the middle abdominal region, cranial to pelvic
limb.
11.Lateral Mesoderm – tissue that gives rise to
limbs and body wall.
12.Vitelline Arteria / Vein – important role in
circulation of blood to and from the yolk sac of a
fetus.
13.Somite – gives rise to cells that forms vertebral,
rib structures. Dermis and skeletal muscles.
14.Spine – back bone, vertebral column structure
15.Tail fold – bending of the caudal aspect of
embryo ventrally that repositions cloaca and
Figure 3. Stained Whole Mount of a 48 Hour Chick Embryo
allantois.
ACTIVITY 10
Cross Sections of a 48 Hour Chick Embryo

*Differentiation and divisions of the different structure of the brain; mesencephalon, metencephalon, and myelencephalon
ACTIVITY 10
Cross Sections of a 48 Hour Chick Embryo

*appearance of the cardinal veins, notochord, diencephalon, optic cup + lens and vesicle, branchial arches.
ACTIVITY 10
Cross Sections of a 48 Hour Chick Embryo

*appearance of the rathke’s pocket and oral plate, otic capsule and Pharynx area are now more wider.
ACTIVITY 10
Cross Sections of a 48 Hour Chick Embryo

*appearance of arteries and tissues; endocardium and developing mesodermal layer; epimyocardium .
Telencephalon reduced in its size.
ACTIVITY 10
Cross Sections of a 48 Hour Chick Embryo

*appearance of the spinal cord, laryngotracheal groove, foregut, ventricle, and somites
ACTIVITY 10
Cross Sections of a 48 Hour Chick Embryo

*ventricle is reduced in its size. appearance of liver diverticula and coelom, disappearance of ventricle and sinus venosus
ACTIVITY 10
Cross Sections of a 48 Hour Chick Embryo

*appearance of chorion, amniotic cavity, and intestinal portal


ACTIVITY 10
Question No. 1 Name and describe the structures developed in the 48 hour
embryo; what is the most prominent structures and its importance?

✓ Area opaca and pellucida are not visible


✓ The embryonic area has grown in size
✓ Primitive streak disappeared
✓ The mesoderm in front of Hensen’s node has given rise to 16 to 28 pairs of somites
✓ The brain has differentiated into telencephalon, prosencephalon, mesencephalon,
metencephalon, and myelencephalon
✓ The heart has been differentiated into ventricle and atrium. Sinus venosus and truncus
arteriosus has already started developing.
✓ The eye has been differentiated into optic cup, lens, optic vesicle has also developed sufficiently
✓ The head region has curved on right side due to cranial flexion
✓ 3 pharyngeal gill slits have also been differentiated
✓ Behind Hensen’s node, a tail bud has also developed
✓ Lateral amniotic folds, anterior omphalomesenteric vein and vitelline artery have appeared
ACTIVITY 10
Generalization
As a summary of the activity, a 48 hour chick
embryo was observed, identified and learned its
different structures and parts during the
development and growth of the embryo after
fertilization. The difference of the embryonic
development in 48 hours can be clearly seen in
the figures presented previously. We can see the
size of the embryo doubled, it has turned its head
right towards the yolk, its left side of its head rest
on the yolk sac (head fold of an amnion), vitelline
veins are spreading and already has 26 to 28
pairs of somites.
Thank you for listening!
ACTIVITY 10
The Chick Embryonic
Development in 48 Hours
Group 4 – CORUNA, GUNHURAN, HERBOLINGO, SANTIAGO
NS Embryology - Laboratory

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